CN108192854A - A kind of richness manganese yeast product and its production method - Google Patents
A kind of richness manganese yeast product and its production method Download PDFInfo
- Publication number
- CN108192854A CN108192854A CN201810097815.4A CN201810097815A CN108192854A CN 108192854 A CN108192854 A CN 108192854A CN 201810097815 A CN201810097815 A CN 201810097815A CN 108192854 A CN108192854 A CN 108192854A
- Authority
- CN
- China
- Prior art keywords
- manganese
- culture
- medium
- content
- yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/36—Adaptation or attenuation of cells
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of rich manganese yeast product and its production methods.The richness manganese yeast product manganese content is every kilogram of 2000~5000 milligrams of yeast.Production method is to prepare the barms of resistance to Gao Meng first, then carries out slant strains culture successively, level liquid seed culture, secondary liquid seed culture, fermentation tank culture, separation drying, crushes, rich manganese yeast product.Richness manganese yeast product of the invention is deposited without influencing, and can significantly drop manganese element excretion in Broiler chicks excrement on manganese element in broiler growth performance and tissue, so as to prove validity of the rich manganese yeast product for Broiler chicks healthy growth and environmental protection.
Description
Technical field
The invention belongs to field of feed additive technology, and in particular to a kind of rich manganese yeast product for animal feed and
Its production method.
Background technology
Manganese is the essential element of life entity, and closely related with carbohydrate and fat metabolism, manganese can promote bone
Growth and development, protect the complete of particulate body in cell, keep normal brain function, maintain normal glycometabolism and fatty generation
It thanks, the hematopoiesis function of human body can be improved.During animal manganese deficiency, feed intake declines；Growth and development is obstructed；Skeleton deformity, arthrocele,
Osteoporosis.It is not normal that jenny manganese deficiency mainly shows out of heat or sexual cycle, is not easy to become pregnant, and Early placental phase is miscarried or produced weak
Tire, stillborn foetus, monster.The main path of the supplement manganese of animal feeding at present is that required manganese is additionally added in feed, it is most of with
Based on inorganic manganese.Inorganic manganese absorption rate is relatively low, the manganese of excessive addition in feed, not only increase production cost but also
The deposition of manganese in excrement can be increased, environmental pollution is caused to ecological environment.In livestock and poultry cultivation production, the row of the trace element such as manganese
Put one of urgent problem to be solved for being increasingly becoming puzzlement agricultural sustainable development.
At present, it is domestic mostly using inorganic manganeses such as high dose manganese oxide, manganese sulfates as manganese nutrition additive, but inorganic manganese
Biological value is low, additive amount is big, has easily caused serious environmental pollution；And the amino acid that stability is good, biological value is higher
Complexing manganese salt (such as manganese methionine) is since its production technology is complicated, higher price limits its application.And rich manganese yeast not only may be used
To meet needs of the animal body for manganese, and there is very abundant nutritional ingredient in yeast, synergistic effect can be played.
Invention content
The object of the present invention is to provide a kind of rich manganese yeast product and its production methods, are provided for animal safe and efficient
Manganese-supplementing agent.
In order to achieve the above objectives, inventor must solve the problems, such as two it is critical, first, preparing the saccharomycete of resistance to Gao Meng
Kind；Second is that the production technology of high Mn content richness manganese yeast.
The production method of richness manganese yeast product of the invention includes the following steps：
1) barms of resistance to Gao Meng are prepared
1.1) the malt sugar culture-medium of different manganese concentration is respectively configured, it is dense that brewer's yeast strain is inoculated with different manganese respectively
It is cultivated in the malt sugar culture-medium of degree；The highest malt sugar culture-medium of solubility containing manganese of strain part survival is selected after culture；
Strain is survived in the malt sugar culture-medium as mutagenic species；
1.2) mutagenic species are inoculated on Induced medium and carry out 24~48h of mutagenesis culture；
1.3) bacterial strain after mutagenesis is subjected to separation screening, is inoculated into the malt sugar culture-medium containing manganese and cultivates, filter out
The strong strain of anti-manganese ability, as resistance to high manganese richness manganese barms；
2) production technology of high Mn content richness manganese yeast
2.1) slant strains culture：Resistance to high manganese richness manganese barms are inoculated in sugared content 5~20%, 60~180 μ of manganese content
G/ml, on the brewer's wort solid medium of pH value 4.5~5.5,30 DEG C of cultivation temperature cultivates 24~48h, obtains inclined-plane saccharomycete
Kind；
2.2) level liquid seed culture：Inclined-plane barms are diluted to bacterial strain 4,960,000/ml of number, 2ml dilutions is taken to connect
Kind in equipped with 250ml sugared contents 5~20%, manganese content 60~180 μ g/ml, the three of the malt extract medium of pH value 4.5~5.5
In the bottle of angle, 30 DEG C of cultivation temperature cultivates 24~48h, obtains level liquid seed culture fluid；
2.3) secondary liquid seed culture：100ml level liquid seed culture fluids are inoculated in and are contained equipped with 50~100L sugar
Amount 5~20%, 60~180 μ g/ml of manganese content, in the fermentation tank of the malt extract medium of pH value 4.5~5.5, cultivation temperature 30
DEG C, 24~48h is cultivated, obtains secondary liquid seed culture fluid；
2.4) fermentation tank culture：1000ml secondary liquid seed culture fluids are inoculated in equipped with 1000~2000L sugared contents 5
~20%, 60~180 μ g/ml of manganese content, in the fermentation tank of the malt extract medium of pH value 4.5~5.5,30 DEG C of cultivation temperature,
24~48h is cultivated, obtains rich manganese Yeast Cultivation liquid；
2.5) drying is detached：Seperator detaches, 60~90 DEG C of drying；
2.6) it crushes：Pulverizer had both obtained rich manganese yeast product after crushing, manganese content is every kilogram of yeast 2000~5000
Milligram.
The total sugar content 5~20% of the malt sugar culture-medium containing manganese in the step 1.1) and step 1.3), containing manganese
60~180 μ g/ml, pH value 4.5~5.5 are measured, 30 DEG C of cultivation temperature cultivates 24~48h.
The manganese content of the malt sugar culture-medium containing manganese in the step 1.3) is higher than the wheat containing manganese in step 1.1)
Bud sugar culture-medium.
Mutagens in Induced medium in the step 1.2) are N-methyl-N-nitro-N-nitrosoguanidine, concentration
1.8~3.2mg/mL, total sugar content 5~20%, pH value 4.0~7.0.
Manganese contained by the malt sugar culture-medium containing manganese be inorganic manganese, the inorganic manganese include manganese oxide, sulfate or
Chloride salt；The nitrogen source of the malt sugar culture-medium and Induced medium is in urea, ammonium nitrate, ammonium sulfate and phosphoric acid hydrogen ammonia
It is one or more of；The phosphorus source of the malt sugar culture-medium and Induced medium is phytic acid calcium, phosphoric acid hydrogen ammonia, dihydrogen phosphate, phosphorus
One or more of sour hydrogen dipotassium.
Specific embodiment
Embodiment 1
1) barms according to different biochemical characteristics are inoculated into the culture medium of different manganese concentration and cultivate, and filter out anti-manganese
The strong barms of ability, as mutagenic species.
2) the strong barms of anti-manganese ability are inoculated on the nitroso culture medium of various dose and carry out mutagenesis；
3) bacterial strain of mutagenesis is subjected to separation screening, is inoculated into the culture medium of different manganese concentration and cultivates, filter out biology
The strong strain of the higher and anti-manganese ability of amount, as resistance to high manganese richness manganese barms；
4) screening and culturing medium and condition of culture
Malt sugar culture-medium, total sugar content 5~20%, containing manganese (inorganic manganese salt) measure 60~180 μ g/ml, pH value 4.5~
5.5,30 DEG C of cultivation temperature cultivates 24~48h.
Embodiment 2
Rich manganese yeast production technique：Slant strains culture → level liquid seed culture → secondary liquid seed culture → hair
Fermentation tank culture → separation drying → crushing → richness manganese yeast product.
1) slant strains culture：Rich manganese barms are inoculated in sugared content 5~20%, and 60 μ g/ are measured containing manganese (inorganic manganese salt)
Ml, on the brewer's wort solid medium of pH value 4.5~5.5,30 DEG C of cultivation temperature cultivates 24~48h.
2) level liquid seed culture：Inclined-plane barms are inoculated in 50 equipped with 250ml sugared contents 5~20%, are contained
Manganese (inorganic manganese salt) measures 60 μ g/ml, in the triangular flask of the malt extract medium of pH value 4.5~5.5,30 DEG C of cultivation temperature, and culture
24~48h.
3) secondary liquid seed culture：Level liquid seed culture fluid is inoculated in equipped with 50~100L sugared contents 5~
20%, measure 60 μ g/ml, in the fermentation tank of the malt extract medium of pH value 4.5~5.5, cultivation temperature 30 containing manganese (inorganic manganese salt)
DEG C, cultivate 24~48h.
4) fermentation tank culture：Secondary liquid seed culture fluid is inoculated in equipped with 1000~2000L sugared contents 5~20%,
60 μ g/ml are measured containing manganese (inorganic manganese salt), in the fermentation tank of the malt extract medium of pH value 4.5~5.5,30 DEG C of cultivation temperature, manganese
It adds in batches, cultivates 24~48h.
5) drying is detached：Seperator detaches, 60~90 DEG C of drying.
6) it crushes：Pulverizer had both obtained high Mn content richness manganese yeast after crushing, manganese content is every kilogram of 2000 milligrams of yeast
Left and right.
Embodiment 3
Rich manganese yeast production technique：Slant strains culture → level liquid seed culture → secondary liquid seed culture → hair
Fermentation tank culture → separation drying → crushing → richness manganese yeast product.
1) slant strains culture：Rich manganese barms are inoculated in sugared content 5~20%, and 100 μ g/ are measured containing manganese (inorganic manganese salt)
Ml, on the brewer's wort solid medium of pH value 4.5~5.5,30 DEG C of cultivation temperature cultivates 24~48h.
2) level liquid seed culture：Inclined-plane barms are inoculated in 50 equipped with 250ml sugared contents 5~20%, are contained
Manganese (inorganic manganese salt) measures 100 μ g/ml, in the triangular flask of the malt extract medium of pH value 4.5~5.5,30 DEG C of cultivation temperature, and culture
24~48h.
3) secondary liquid seed culture：Level liquid seed culture fluid is inoculated in equipped with 50~100L sugared contents 5~
20%, measure 100 μ g/ml, in the fermentation tank of the malt extract medium of pH value 4.5~5.5, cultivation temperature 30 containing manganese (inorganic manganese salt)
DEG C, cultivate 24~48h.
4) fermentation tank culture：Secondary liquid seed culture fluid is inoculated in equipped with 1000~2000L sugared contents 5~20%,
100 μ g/ml are measured containing manganese (inorganic manganese salt), in the fermentation tank of the malt extract medium of pH value 4.5~5.5,30 DEG C of cultivation temperature, manganese
It adds in batches, cultivates 24~48h.
5) drying is detached：Seperator detaches, 60~90 DEG C of drying.
6) it crushes：Pulverizer had both obtained high Mn content richness manganese yeast after crushing, manganese content is every kilogram of 3000 milligrams of yeast.
Embodiment 4
Rich manganese yeast production technique：Slant strains culture → level liquid seed culture → secondary liquid seed culture → hair
Fermentation tank culture → separation drying → crushing → richness manganese yeast product.
1) slant strains culture：Rich manganese barms are inoculated in sugared content 5~20%, and 140 μ g/ are measured containing manganese (inorganic manganese salt)
Ml, on the brewer's wort solid medium of pH value 4.5~5.5,30 DEG C of cultivation temperature cultivates 24~48h.
2) level liquid seed culture：Inclined-plane barms are inoculated in 50 equipped with 250ml sugared contents 5~20%, are contained
Manganese (inorganic manganese salt) measures 140 μ g/ml, in the triangular flask of the malt extract medium of pH value 4.4~5.5,30 DEG C of cultivation temperature, and culture
24~48h.
3) secondary liquid seed culture：Level liquid seed culture fluid is inoculated in equipped with 50~100L sugared contents 5~
20%, measure 140 μ g/ml, in the fermentation tank of the malt extract medium of pH value 4.4~5.5, cultivation temperature 30 containing manganese (inorganic manganese salt)
DEG C, cultivate 24~48h.
4) fermentation tank culture：Secondary liquid seed culture fluid is inoculated in equipped with 1000~2000L sugared contents 5~20%,
140 μ g/ml are measured containing manganese (inorganic manganese salt), in the fermentation tank of the malt extract medium of pH value 4.5~5.5,30 DEG C of cultivation temperature, manganese
It adds in batches, cultivates 24~48h.
5) drying is detached：Seperator detaches, 60~90 DEG C of drying.
6) it crushes：Pulverizer had both obtained high Mn content richness manganese yeast after crushing, manganese content is every kilogram of 4000 milligrams of yeast.
Embodiment 5
Rich manganese yeast production technique：Slant strains culture → level liquid seed culture → secondary liquid seed culture → hair
Fermentation tank culture → separation drying → crushing → richness manganese yeast product.
1) slant strains culture：Rich manganese barms are inoculated in sugared content 5~20%, and 180 μ g/ are measured containing manganese (inorganic manganese salt)
Ml, on the brewer's wort solid medium of pH value 4.5~5.5,30 DEG C of cultivation temperature cultivates 24~48h.
2) level liquid seed culture：Inclined-plane barms are inoculated in 50 equipped with 250ml sugared contents 5~20%, are contained
Manganese (inorganic manganese salt) measures 180 μ g/ml, in the triangular flask of the malt extract medium of pH value 4.5~5.5,30 DEG C of cultivation temperature, and culture
24~48h.
3) secondary liquid seed culture：Level liquid seed culture fluid is inoculated in equipped with 50~100L sugared contents 5~
20%, measure 180 μ g/ml, in the fermentation tank of the malt extract medium of pH value 4.5~5.5, cultivation temperature 30 containing manganese (inorganic manganese salt)
DEG C, cultivate 24~48h.
4) fermentation tank culture：Secondary liquid seed culture fluid is inoculated in equipped with 1000~2000L sugared contents 5~20%,
180 μ g/ml are measured containing manganese (inorganic manganese salt), in the fermentation tank of the malt extract medium of pH value 4.5~5.5,30 DEG C of cultivation temperature, manganese
It adds in batches, cultivates 24~48h.
5) drying is detached：Seperator detaches, 60~90 DEG C of drying.
6) it crushes：Pulverizer had both obtained high Mn content richness manganese yeast after crushing, manganese content is every kilogram of 5000 milligrams of yeast.
Embodiment 6
1 experimental animal
Experimental animal：Using 1 age in days AA Broiler chicks 200, according to genetic background is identical, weight is close, nest is not identical
Principle is randomly divided into 10 groups, and every group of 4 repetitions each repeat 5 chickens.
2 test materials
2.1 rich manganese yeast products
Rich manganese yeast product, manganese content 5000mg/Kg are prepared by 5 method of embodiment.
2.2 experiment daily rations
Test group daily ration is prepared according to inventive formulation, and feed formula is specially：Corn 65%；Dregs of beans 26%；
Fish meal 5.99%；Methionine 0.16%；Salt 0.25%；Mountain flour 1.14%；In the premix also comprising vitamins and
Trace element, wherein the additive capacity of Mn elements is respectively in every kilogram of daily ration：180mg/Kg.Manganese element in test group daily ration
Addition form is yeast manganese, and the addition form of Mn elements is manganese sulfate and manganese acetate in control group daily ration.
3 test methods
Using 3 × 3 liang of factor completely randomized designs, 3 kinds of manganese sources are respectively manganese sulfate, manganese acetate and yeast manganese, 3 manganese water
It is flat be respectively 60,120,180mg/kg, form 10 groups (1 control group, 9 processing groups) altogether.
200 1 age in days healthy AA Broiler chicks are randomly divided into 10 groups, every group of 4 repetitions each repeat 5 chickens.Raising
In in a stainless steel plating modeling broiler chicken cage, test three weeks, carried out disinfection, press to henhouse, chicken coop, sink and hopper before on-test
Immune programme is immunized.It is freely eaten and drinking-water, the 3rd weekend claims chicken empty body weight and feed consumption rate to repeat cage as unit.
The acquisition and preparation of 1.3 samples
21 age in days mornings next day weighed on an empty stomach, and chicken Culling heart blood, anticoagulant heparin prepares blood plasma.Taken out immediately after butchering the heart,
Liver, kidney, with normal saline flushing for several times, after filter paper blots, be put into label self-sealing plastic bag, myocardium -80 DEG C of liquid nitrogen cryopreservations, with
The myocardium manganese content of back-up analysis.- 20 DEG C of its hetero-organization and blood plasma freeze, in case analysis.
The analysis of 1.4 samples
Diet and the measure for organizing manganese content:Diet, cardiac muscle and other histoorgans are done with concentrated nitric acid and concentrated hydrochloric acid
Method digests, and manganese content therein is measured with WFX-IE2 types atomic absorption spectrophotometer.
1.5 measure project
To organize as unit weight during on-test, at the end of by only weighing, and weigh residual feed, it is average to calculate with this
Daily gain, average daily gain, feed efficiency etc. evaluate the shadow of different manganese sources and manganese level to broiler growth performance
It rings.
Sampling is butchered during off-test, measures content of hemoglobin, liver, cardiac muscle, kidney manganese concentration.
1.6 data preparations and statistical analysis
All test datas are carried out with variance analysis with GLM programs in SAS softwares, significant difference person is relatively more flat with LSD methods
The significance of difference between mean；Biology of the Organic Mn source relative to manganese sulfate is calculated with existing multiple linear regression slope ratio method
Learn utilization rate.
2 results and discussion
The influence of 2.1 daily ration manganese sources and manganese Levels Upon Performance of Broilers
The result of the test of Broiler chicks production performance is shown in Table 2.Different manganese sources are equal to Broiler chicks items production performance Index Influence
Not significantly (P>0.05)；But it takes temperature from feeding, rich manganese yeast group is higher than manganese sulfate group and manganese acetate group.In terms of daily gain, control
Group is substantially less than the processing group (P that 180mg/kg manganese is added in daily ration with other two test groups<0.05), other each processing
Not notable (the P of difference between group>0.05).From the point of view of feed intake, control group significant difference (P compared with other processing groups<0.05),
And not notable (the P of comparing difference between processing group>0.05)；In terms of feed efficiency, between control group and each processing group
Difference not significantly (P>0.05).This experiment production performance the result shows that：Feed the growth performance of different manganese source Broiler chicks
Influence not significantly (P>0.05).
The influence of 2 daily ration manganese source of table and manganese Levels Upon Performance of Broilers
1. data are M ± SD in table, similarly hereinafter；2. manganese source conspicuousness compares between referring to 3 kinds of manganese sources, similarly hereinafter；It is 3. horizontal notable
Property compares between referring to 3 Mn sources, similarly hereinafter；4. alphabetical identical person's difference is not notable, the capitalization level of signifiance is 0.01,
Lowercase is 0.05, similarly hereinafter.
2.2 daily ration manganese sources and manganese level are to the shadow of MnSOD activity in Broiler chicks cardiac muscle manganese content, cardiac muscle cell's mitochondria
Ring
From table 3, different manganese sources influence Broiler chicks cardiac muscle manganese concentration not significantly (P>0.05), different manganese levels are to meat
Chick cardiac muscle manganese has pole to significantly affect (P<0.01), myocardium manganese concentration increases with the increase of Mn Levels in Diet；
The influence of 3 daily ration manganese source of table and manganese level to Broiler chicks tissue manganese concentration
It is shown as from 3,3 manganese source groups of table, increased with Mn Levels in Diet, liver, cardiac muscle, kidney manganese concentration increase
Add.Different manganese sources influence Broiler chicks liver manganese concentration not significantly (P>0.05), different manganese levels have pole significant difference (P<
0.01)。
The influence that 4 Zinc-rich saccharomyces cerevisiae of the present invention of table drains store pig Zn-ef ficiency
By table 4 as it can be seen that 3 manganese source groups are shown as, increase with Mn Levels in Diet, excrement manganese concentration increases.Manganese acetate phase
The manganese concentration in excrement, and significant difference (P are reduced for manganese sulfate<0.05)；Rich manganese yeast is reduced relative to manganese sulfate
Manganese concentration in excrement, and difference extremely significantly (P<0.01).
5. conclusion
Richness manganese yeast product of the invention is deposited without significantly affecting, and can on manganese element in broiler growth performance and tissue
To significantly reduce manganese element excretion in Broiler chicks chicken manure, so as to prove rich manganese yeast product for Broiler chicks healthy growth and guarantor
The validity in retaining ring border.
Claims (7)
1. a kind of production method of richness manganese yeast product, it is characterised in that include the following steps：
1) barms of resistance to Gao Meng are prepared
1.1) the malt sugar culture-medium of different manganese concentration is respectively configured, brewer's yeast strain is inoculated with different manganese concentration respectively
Malt sugar culture-medium in cultivate；The highest malt sugar culture-medium of solubility containing manganese of strain part survival is selected after culture；The wheat
Strain is survived in bud sugar culture-medium as mutagenic species；
1.2) mutagenic species are inoculated on Induced medium and carry out 24~48h of mutagenesis culture；
1.3) bacterial strain after mutagenesis is subjected to separation screening, is inoculated into the malt sugar culture-medium containing manganese and cultivates, filter out anti-manganese
The strong strain of ability, as resistance to high manganese richness manganese barms；
2) production technology of high Mn content richness manganese yeast
2.1) slant strains culture：Resistance to high manganese richness manganese barms are inoculated in sugared content 5~20%, 60~180 μ g/ of manganese content
Ml, on the brewer's wort solid medium of pH value 4.5~5.5,30 DEG C of cultivation temperature cultivates 24~48h, obtains inclined-plane saccharomycete
Kind；
2.2) level liquid seed culture：Inclined-plane barms are diluted to bacterial strain 4,960,000/ml of number, 2ml dilutions is taken to be inoculated in
Equipped with 250ml sugared contents 5~20%, 60~180 μ g/ml of manganese content, the triangular flask of the malt extract medium of pH value 4.5~5.5
In, 30 DEG C of cultivation temperature cultivates 24~48h, obtains level liquid seed culture fluid；
2.3) secondary liquid seed culture：100ml level liquid seed culture fluids are inoculated in equipped with 50~100L sugared contents 5~
20%, 60~180 μ g/ml of manganese content, in the fermentation tank of the malt extract medium of pH value 4.5~5.5,30 DEG C of cultivation temperature, training
24~48h is supported, obtains secondary liquid seed culture fluid；
2.4) fermentation tank culture：1000ml secondary liquid seed culture fluids are inoculated in equipped with 1000~2000L sugared contents 5~
20%, 60~180 μ g/ml of manganese content, in the fermentation tank of the malt extract medium of pH value 4.5~5.5,30 DEG C of cultivation temperature, training
24~48h is supported, obtains rich manganese Yeast Cultivation liquid；
2.5) drying is detached：Seperator detaches, 60~90 DEG C of drying；
2.6) it crushes：Pulverizer had both obtained rich manganese yeast product after crushing, manganese content is every kilogram of 2000~5000 milligrams of yeast.
2. production method as described in claim 1, it is characterised in that in the step 1.1) and step 1.3) containing manganese
The total sugar content 5~20% of malt sugar culture-medium, 60~180 μ g/ml of manganese content, pH value 4.5~5.5,30 DEG C of cultivation temperature, training
Support 24~48h.
3. production method as described in claim 1, it is characterised in that the maltose culture containing manganese in the step 1.3)
The manganese content of base is higher than the malt sugar culture-medium containing manganese in step 1.1).
4. production method as described in claim 1, it is characterised in that luring in the Induced medium in the step 1.2)
Change agent is N-methyl-N-nitro-N-nitrosoguanidine, 1.8~3.2mg/mL of concentration, total sugar content 5~20%, and pH value 4.0~
7.0。
5. production method as described in claim 1, it is characterised in that manganese contained by the malt sugar culture-medium containing manganese is inorganic
Manganese, the inorganic manganese include manganese oxide, sulfate or chloride salt；The nitrogen source of the malt sugar culture-medium and Induced medium
For one or more of urea, ammonium nitrate, ammonium sulfate and phosphoric acid hydrogen ammonia；The phosphorus of the malt sugar culture-medium and Induced medium
Source is one or more of phytic acid calcium, phosphoric acid hydrogen ammonia, dihydrogen phosphate, dipotassium hydrogen phosphate.
6. a kind of rich manganese yeast product that method as described in claim 1 is prepared.
7. application of the rich manganese yeast product in animal feed is prepared described in claim 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810097815.4A CN108192854A (en) | 2018-01-31 | 2018-01-31 | A kind of richness manganese yeast product and its production method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810097815.4A CN108192854A (en) | 2018-01-31 | 2018-01-31 | A kind of richness manganese yeast product and its production method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108192854A true CN108192854A (en) | 2018-06-22 |
Family
ID=62592169
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810097815.4A Pending CN108192854A (en) | 2018-01-31 | 2018-01-31 | A kind of richness manganese yeast product and its production method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108192854A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1141375C (en) * | 2001-01-17 | 2004-03-10 | 中国科学院微生物研究所 | Se-enriched high-biomass yeast and its preparing process |
| CN1142266C (en) * | 2001-01-17 | 2004-03-17 | 中国科学院微生物研究所 | Cr-enriched yeast containing high biomass and its preparing process |
| CN101508961A (en) * | 2009-03-16 | 2009-08-19 | 浙江大学 | Method of preparing yeast rich in copper |
| CN101629146B (en) * | 2009-08-07 | 2011-07-20 | 中国农业科学院兰州畜牧与兽药研究所 | Zinc-rich yeast with high biomass, breeding selection method and application thereof |
| CN101575579B (en) * | 2008-05-06 | 2012-01-04 | 安琪酵母股份有限公司 | Ferrum-rich saccharomyces cerevisiae and production method thereof |
| CN101575580B (en) * | 2008-05-06 | 2012-01-18 | 安琪酵母股份有限公司 | Zinc-rich saccharomyces cerevisiae and production method thereof |
| CN101611135B (en) * | 2007-02-16 | 2012-06-20 | 雪印惠乳业株式会社 | Agent for improving viability of lactic acid bacterium |
| CN103667086A (en) * | 2013-12-04 | 2014-03-26 | 北京大北农科技集团股份有限公司 | Selenium-enriched yeast with high biomass, and additive and premix containing the same |
| CN107119038A (en) * | 2017-05-05 | 2017-09-01 | 浙江大学 | A kind of Zinc-rich saccharomyces cerevisiae product and its production method |
| CN107236677A (en) * | 2017-05-15 | 2017-10-10 | 浙江大学 | A kind of ferrum-rich saccharomyces cerevisiae product and its production method |
-
2018
- 2018-01-31 CN CN201810097815.4A patent/CN108192854A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1141375C (en) * | 2001-01-17 | 2004-03-10 | 中国科学院微生物研究所 | Se-enriched high-biomass yeast and its preparing process |
| CN1142266C (en) * | 2001-01-17 | 2004-03-17 | 中国科学院微生物研究所 | Cr-enriched yeast containing high biomass and its preparing process |
| CN101611135B (en) * | 2007-02-16 | 2012-06-20 | 雪印惠乳业株式会社 | Agent for improving viability of lactic acid bacterium |
| CN101575579B (en) * | 2008-05-06 | 2012-01-04 | 安琪酵母股份有限公司 | Ferrum-rich saccharomyces cerevisiae and production method thereof |
| CN101575580B (en) * | 2008-05-06 | 2012-01-18 | 安琪酵母股份有限公司 | Zinc-rich saccharomyces cerevisiae and production method thereof |
| CN101508961A (en) * | 2009-03-16 | 2009-08-19 | 浙江大学 | Method of preparing yeast rich in copper |
| CN101629146B (en) * | 2009-08-07 | 2011-07-20 | 中国农业科学院兰州畜牧与兽药研究所 | Zinc-rich yeast with high biomass, breeding selection method and application thereof |
| CN103667086A (en) * | 2013-12-04 | 2014-03-26 | 北京大北农科技集团股份有限公司 | Selenium-enriched yeast with high biomass, and additive and premix containing the same |
| CN107119038A (en) * | 2017-05-05 | 2017-09-01 | 浙江大学 | A kind of Zinc-rich saccharomyces cerevisiae product and its production method |
| CN107236677A (en) * | 2017-05-15 | 2017-10-10 | 浙江大学 | A kind of ferrum-rich saccharomyces cerevisiae product and its production method |
Non-Patent Citations (9)
| Title |
|---|
| M. R. CALOMME 等: "Selenium and Lactobacillus species", 《JOURNAL OF APPLIED BACTERIOLOGY》 * |
| 全国饲料添加剂信息站: "矿物元素饲料添加剂-酵母锰", 《中国饲料添加剂》 * |
| 凌明亮 等编著: "《饲料添加剂开发与应用技术》", 30 April 2006, 科学技术文献出版社 * |
| 刘杰 等: "富硒酵母的研究进展", 《饲料工业》 * |
| 张文学 主编: "《生态食品工程学》", 31 October 2006, 四川大学出版社 * |
| 杨振海 等主编: "《饲料添加剂安全使用规范》", 30 September 2003, 中国农业出版社 * |
| 王旭 等: "富微量元素酵母及培养条件的研究", 《中国卫生检验杂志》 * |
| 甘孟侯: "《现代兽医兽药大全》", 30 November 2014, 中国农业大学出版社 * |
| 马娅娅: "有机锰对正常和感染球虫肉仔鸡生产性能、免疫功能及抗氧化能力的影响", 《中国优秀硕士学位论文全文数据库（电子期刊）农业科技辑》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103960547B (en) | A kind of low albumen ablactational baby pig feedstuff and preparation method thereof | |
| CN104187085B (en) | A kind of channel catfish immunity enhancement type feedstuff | |
| CN103284017B (en) | Application of yucca in compound feed for megalobrama amblycephala and formula of feed | |
| CN103027187A (en) | Anti-stress fermentation protein feed and producing method thereof | |
| CN102138650A (en) | Wheat type complete feed for gosling | |
| CN102669427B (en) | Paecilomyces militaris Liang sp.nov. fermented feed additive as well as preparation method and application thereof | |
| CN104872384B (en) | Needle mushroom foot feed addictive | |
| CN103289907A (en) | A candida utilis protein product and applications thereof in breeding industry | |
| CN103392917A (en) | Composite feed additive preparation technology based on gynostemma pentaphylla | |
| CN106190912A (en) | Photosynthetic bacteria microbial inoculum and preparation method and application | |
| CN107865217A (en) | A kind of sheep feed and preparation method thereof | |
| CN102090544B (en) | Selenium-rich goose feed and method for improving organic selenium in goose bodies | |
| CN106306582A (en) | Zinc-enriched feed for adult lobsters | |
| CN105851658A (en) | Functional layer feed | |
| CN102669426B (en) | Penicillium paecilomyces cicadae (miquel) samson fermentation product used as feed additive as well as preparation method and application thereof | |
| CN108048386A (en) | A kind of yeast rich in copper product and its production method | |
| CN107119038A (en) | A kind of Zinc-rich saccharomyces cerevisiae product and its production method | |
| CN107236677A (en) | A kind of ferrum-rich saccharomyces cerevisiae product and its production method | |
| CN107348222A (en) | A kind of preparation method of livestock culture feed | |
| CN103734497B (en) | A kind of feed addictive of anti-cow heat stress | |
| CN103202386A (en) | Application of Lactobacillus casei fermentation liquor to feeds | |
| CN105394454A (en) | Feed additive for trionyx sinensis | |
| CN105941914A (en) | Fattening pig feed | |
| CN102726603A (en) | Method for preparing feed additive by fermentation waste of trollflower dregs | |
| CN108192854A (en) | A kind of richness manganese yeast product and its production method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180622 |
|
| RJ01 | Rejection of invention patent application after publication |