CN108192825A - Suitable for single celled Tissue Culture Dish - Google Patents

Suitable for single celled Tissue Culture Dish Download PDF

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Publication number
CN108192825A
CN108192825A CN201810234439.9A CN201810234439A CN108192825A CN 108192825 A CN108192825 A CN 108192825A CN 201810234439 A CN201810234439 A CN 201810234439A CN 108192825 A CN108192825 A CN 108192825A
Authority
CN
China
Prior art keywords
ware
lid
hole
buckle
rubber pad
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201810234439.9A
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Chinese (zh)
Inventor
吴东颖
董红宇
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Weifang Xia Wei Tested Biological Technology Co Ltd
Original Assignee
Weifang Xia Wei Tested Biological Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Weifang Xia Wei Tested Biological Technology Co Ltd filed Critical Weifang Xia Wei Tested Biological Technology Co Ltd
Priority to CN201810234439.9A priority Critical patent/CN108192825A/en
Publication of CN108192825A publication Critical patent/CN108192825A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/38Caps; Covers; Plugs; Pouring means
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/46Means for fastening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04Filters; Permeable or porous membranes or plates, e.g. dialysis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/02Filters

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to technical field of cell culture, it is especially a kind of to be suitable for single celled Tissue Culture Dish, ware body including ware lid and with accommodating chamber, there is step surface positioned at the top of accommodating chamber on ware body, matched ring-shaped rubber pad is fixed on step surface, buckle is fixed on the top end face of ware body, the peripheral surface of ware lid is outward extended with flank, ware lid is placed on ring-shaped rubber pad, ware covers the through-hole for running through and having and being connected with accommodating chamber, matched filter membrane is provided in through-hole, the flank of ware lid is blocked by buckle, and so that the flank of ware lid is tight against on buckle under the elastic force effect of ring-shaped rubber pad, it is slid from ware body so as to effectively prevent ware lid;The size of through-hole breathing area is adjusted by rotating port lid, so as to adapt to that the oxygen demand of cell is cultivated in ware body, in addition filter membrane may filter that the dirt in air, and air is avoided to pollute the culture solution in accommodating chamber in ware body.

Description

Suitable for single celled Tissue Culture Dish
Technical field
The present invention relates to technical field of cell culture, especially a kind of to be suitable for single celled Tissue Culture Dish.
Background technology
It is aerobic-type to have more type in unicellular, and the ware lid of existing Tissue Culture Dish is generally direct placement On ware body, and be directly entered between ware lid and ware body there are gap for outside air, the easy to carry dirt of outside air into Enter in culture dish, culture solution is polluted, and when moving, ware lid easily slides from ware body.
Invention content
The technical problem to be solved by the present invention is to:Enter training to solve the easy to carry dirt of outside air in the prior art It supports in ware, culture solution is polluted, and when moving, the problem of ware lid easily slides from ware body now provides a kind of be applicable in In single celled Tissue Culture Dish.
The technical solution adopted by the present invention to solve the technical problems is:One kind is suitable for single celled Tissue Culture Dish, Ware body including ware lid and with accommodating chamber, the top of accommodating chamber is located on the ware body has a step surface, on the step surface Matched ring-shaped rubber pad is fixed with, buckle, the buckle and annular rubber are fixed on the top end face of the ware body There is gap, the peripheral surface of the ware lid is outward extended with flank, and the ware lid is placed on ring-shaped rubber pad, institute between rubber cushion Flank is stated between buckle and ring-shaped rubber pad, the ware is covered through there is the through-hole that is connected with accommodating chamber, in the through-hole Matched filter membrane is provided with, the upper surface of the ware lid is provided with to cover the hole for setting through-hole by shaft rotation Lid, the shaft and through-hole eccentric setting, the axis of the shaft are parallel with the axis of through-hole.
The flank of ware lid is blocked in this programme by buckle, and so that the side of ware lid under the elastic force effect of ring-shaped rubber pad The wing slides tight against on buckle so as to effectively prevent ware lid from ware body;Through-hole ventilation face is adjusted by rotating port lid Long-pending size, so as to adapt to that the oxygen demand of cell is cultivated in ware body, in addition filter membrane may filter that the dirt in air, avoid sky Gas pollutes the culture solution in accommodating chamber in ware body.
Since ware body easily slides from operation console, further, the bottom surface of the ware body is fixed with annular non-slip mat, institute The material of annular non-slip mat is stated using rubber, the annular non-slip mat of rubber material can increase the friction between ware body and operation console Power can effectively prevent ware body from being slid from operation console.
Further, the lower surface of the annular non-slip mat is fixed with several anti-skid bulges, and several anti-skid bulges can be effective The annular non-slip mat of increase and operation console between frictional force.
Preferably, the filter membrane is ventilated membrane.
The beneficial effects of the invention are as follows:The present invention's blocks ware lid suitable for single celled Tissue Culture Dish by buckle Flank, and so that the flank of ware lid is tight against on buckle, so as to effectively prevent ware under the elastic force effect of ring-shaped rubber pad Lid slides from ware body;The size of through-hole breathing area is adjusted by rotating port lid, so as to adapt to cultivate cell in ware body Oxygen demand, in addition filter membrane may filter that the dirt in air, air is avoided to cause dirt to the culture solution in accommodating chamber in ware body Dye.
Description of the drawings
The present invention is further described with reference to the accompanying drawings and examples.
Fig. 1 is the side schematic three dimensional views that the present invention is suitable for single celled Tissue Culture Dish;
Fig. 2 is the opposite side schematic three dimensional views that the present invention is suitable for single celled Tissue Culture Dish;
Fig. 3 is the exploded perspective view that the present invention is suitable for single celled Tissue Culture Dish;
Fig. 4 is the schematic top plan view that the present invention is suitable for single celled Tissue Culture Dish;
Fig. 5 be in Fig. 4 A-A to schematic cross-sectional view.
In figure:1st, ware lid, 1-1, flank, 1-2, through-hole, 2, ware body, 2-1, accommodating chamber, 2-2, step surface, 2-3, buckle, 3rd, ring-shaped rubber pad, 4, filter membrane, 5, port lid, 5-1, shaft, 6, annular non-slip mat, 6-1, anti-skid bulge.
Specific embodiment
In conjunction with the accompanying drawings, the present invention is further explained in detail.These attached drawings are simplified schematic diagram, only with Illustration illustrates the basic structure of the present invention, therefore it only shows composition related to the present invention, direction and with reference to (for example, Upper and lower, left and right, etc.) can be only used for helping the description to the feature in attached drawing.Therefore, it is not adopted in restrictive, sense With detailed description below, and claimed theme is only limited by appended claims and its equivalent form Range.
Embodiment 1
As shown in Figs. 1-5, it is a kind of to be suitable for single celled Tissue Culture Dish, the ware including ware lid 1 and with accommodating chamber 2-1 Body 2 has on the ware body 2 positioned at the top of accommodating chamber 2-1 and is fixed with and its phase on step surface 2-2, the step surface 2-2 The ring-shaped rubber pad 3 matched, be fixed on the top end face of the ware body 2 buckle 2-3, the buckle 2-3 and ring-shaped rubber pad 3 it Between there is gap, the peripheral surface of the ware lid 1 is outward extended with flank 1-1, and the ware lid 1 is placed on ring-shaped rubber pad 3, institute Flank 1-1 is stated between buckle 2-3 and ring-shaped rubber pad 3, the through-hole for having and being connected with accommodating chamber 2-1 is run through on the ware lid 1 Matched filter membrane 4 is provided in 1-2, the through-hole 1-2, the upper surface of the ware lid 1 is set by shaft 5-1 rotations It is equipped with for covering the port lid 5 for setting through-hole 1-2, the shaft 5-1 and through-hole 1-2 eccentric settings, the axis of the shaft 5-1 is with leading to The axis of hole 1-2 is parallel.
Buckle 2-3 and flank 1-1 is respectively provided with two and corresponds, and buckle 2-3 is located at the surface of step surface 2-2.
The bottom surface of the ware body 2 is fixed with annular non-slip mat 6, and the material of the annular non-slip mat 6 is using rubber, rubber material The annular non-slip mat 6 of matter can increase the frictional force between ware body 2 and operation console, can effectively prevent ware body 2 sliding from operation console It falls.
The lower surface of the annular non-slip mat 6 is fixed with several anti-skid bulge 6-1, and several anti-skid bulge 6-1 can be effective Increase the frictional force between annular non-slip mat 6 and operation console.
The filter membrane 4 is ventilated membrane.
When in use, the flank 1-1 of ware lid 1 and the buckle 2-3 on ware body 2 are staggered first, then press down on ware lid 1, ring-shaped rubber pad 3 is compressed downwardly, and then rotates ware lid 1, the lower section that flank 1-1 is made to be located at buckle 2-3, flank 1-1 is located at In gap between buckle 2-3 and ring-shaped rubber pad 3, after unclamping ware lid 1, since the elastic reaction of ring-shaped rubber pad 3 can be by ware The flank 1-1 of lid 1 is tight against on buckle 2-3, so as to fulfill the fixation of ware lid 1;
The present embodiment blocks the flank 1-1 of ware lid 1 by buckle 2-3, and causes under the elastic force effect of ring-shaped rubber pad 3 The flank 1-1 of ware lid 1 slides tight against on buckle 2-3 so as to effectively prevent ware lid 1 from ware body 2;By rotating port lid The size of 5 adjustable through-hole 1-2 breathing areas, so as to adapt to that the oxygen demand of cell is cultivated in ware body 2, in addition filter membrane 4 can The dirt in air filtering is crossed, dust subsidiary in air, dust, fine impurities, miscellaneous bacteria and the entrance of germ is substantially prevented from, keeps away Exempt from air to pollute the culture solution in accommodating chamber 2-1 in ware body 2.
Above-mentioned desirable embodiment according to the present invention is enlightenment, and by above-mentioned description, relevant staff is complete Various changes and amendments can be carried out in the range of without departing from technical thought of the invention.The technical scope of the present invention The content being not limited on specification, it is necessary to which its technical scope is determined according to right.

Claims (4)

1. one kind is suitable for single celled Tissue Culture Dish, it is characterised in that:Including ware lid (1) and with accommodating chamber (2-1) Ware body (2), the top of accommodating chamber (2-1) is located on the ware body (2) has a step surface (2-2), solid on the step surface (2-2) Surely there is matched ring-shaped rubber pad (3), buckle (2-3), the buckle are fixed on the top end face of the ware body (2) There is gap, the peripheral surface of the ware lid (1) is outward extended with flank (1-1), described between (2-3) and ring-shaped rubber pad (3) Ware lid (1) is placed on ring-shaped rubber pad (3), and the flank (1-1) is between buckle (2-3) and ring-shaped rubber pad (3), institute The through-hole (1-2) for running through on ware lid (1) and having and being connected with accommodating chamber (2-1) is stated, is provided in the through-hole (1-2) and matches with it Filter membrane (4), the upper surface of the ware lid (1) is provided with to cover the port lid for setting through-hole (1-2) by shaft (5-1) rotation (5), the shaft (5-1) and through-hole (1-2) eccentric setting, the axis of the axis and through-hole (1-2) of the shaft (5-1) are put down Row.
2. according to claim 1 be suitable for single celled Tissue Culture Dish, it is characterised in that:The bottom of the ware body (2) Face is fixed with annular non-slip mat (6), and the material of the annular non-slip mat (6) uses rubber.
3. according to claim 2 be suitable for single celled Tissue Culture Dish, it is characterised in that:The annular non-slip mat (6) lower surface is fixed with several anti-skid bulges (6-1).
4. according to claim 2 be suitable for single celled Tissue Culture Dish, it is characterised in that:The filter membrane (4) is Ventilated membrane.
CN201810234439.9A 2018-03-21 2018-03-21 Suitable for single celled Tissue Culture Dish Pending CN108192825A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810234439.9A CN108192825A (en) 2018-03-21 2018-03-21 Suitable for single celled Tissue Culture Dish

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201810234439.9A CN108192825A (en) 2018-03-21 2018-03-21 Suitable for single celled Tissue Culture Dish

Publications (1)

Publication Number Publication Date
CN108192825A true CN108192825A (en) 2018-06-22

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CN201810234439.9A Pending CN108192825A (en) 2018-03-21 2018-03-21 Suitable for single celled Tissue Culture Dish

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Country Link
CN (1) CN108192825A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113025494A (en) * 2021-04-25 2021-06-25 河南亚都实业有限公司 Artificial skin independent culture dish and protection structure thereof
CN113755329A (en) * 2021-09-23 2021-12-07 赵宝玉 Cell culture dish

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113025494A (en) * 2021-04-25 2021-06-25 河南亚都实业有限公司 Artificial skin independent culture dish and protection structure thereof
CN113755329A (en) * 2021-09-23 2021-12-07 赵宝玉 Cell culture dish

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