CN108187026A - A kind of product that can promote wound healing - Google Patents
A kind of product that can promote wound healing Download PDFInfo
- Publication number
- CN108187026A CN108187026A CN201810217715.0A CN201810217715A CN108187026A CN 108187026 A CN108187026 A CN 108187026A CN 201810217715 A CN201810217715 A CN 201810217715A CN 108187026 A CN108187026 A CN 108187026A
- Authority
- CN
- China
- Prior art keywords
- content
- soybean protein
- wound healing
- product
- promote wound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000029663 wound healing Effects 0.000 title claims abstract description 24
- 108010073771 Soybean Proteins Proteins 0.000 claims abstract description 55
- 235000019710 soybean protein Nutrition 0.000 claims abstract description 54
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 24
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 9
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229940046009 vitamin E Drugs 0.000 claims abstract description 9
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 9
- 239000011709 vitamin E Substances 0.000 claims abstract description 9
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 claims abstract description 6
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 claims description 16
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 claims description 8
- 229960000458 allantoin Drugs 0.000 claims description 8
- 239000008367 deionised water Substances 0.000 claims description 7
- 229910021641 deionized water Inorganic materials 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 244000068988 Glycine max Species 0.000 claims description 4
- 230000004913 activation Effects 0.000 claims description 3
- 238000004806 packaging method and process Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000003595 mist Substances 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 239000002245 particle Substances 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims 1
- 102000002322 Egg Proteins Human genes 0.000 claims 1
- 108010000912 Egg Proteins Proteins 0.000 claims 1
- 235000014103 egg white Nutrition 0.000 claims 1
- 210000000969 egg white Anatomy 0.000 claims 1
- 208000027418 Wounds and injury Diseases 0.000 abstract description 15
- 206010052428 Wound Diseases 0.000 abstract description 14
- 230000000694 effects Effects 0.000 abstract description 12
- 210000001339 epidermal cell Anatomy 0.000 abstract description 8
- 235000011187 glycerol Nutrition 0.000 abstract description 8
- 238000005507 spraying Methods 0.000 abstract description 7
- 230000035876 healing Effects 0.000 abstract description 6
- 206010072170 Skin wound Diseases 0.000 abstract description 4
- 230000035755 proliferation Effects 0.000 abstract description 4
- 239000002904 solvent Substances 0.000 abstract description 2
- 230000003328 fibroblastic effect Effects 0.000 abstract 1
- 239000004615 ingredient Substances 0.000 abstract 1
- 230000005012 migration Effects 0.000 abstract 1
- 238000013508 migration Methods 0.000 abstract 1
- 229940001941 soy protein Drugs 0.000 abstract 1
- 238000002474 experimental method Methods 0.000 description 9
- 241000699666 Mus <mouse, genus> Species 0.000 description 7
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 230000004663 cell proliferation Effects 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 230000012292 cell migration Effects 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
- 239000011734 sodium Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000011740 C57BL/6 mouse Methods 0.000 description 1
- QXNVGIXVLWOKEQ-UHFFFAOYSA-N Disodium Chemical compound [Na][Na] QXNVGIXVLWOKEQ-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 101710094902 Legumin Proteins 0.000 description 1
- 241000256856 Vespidae Species 0.000 description 1
- 230000001133 acceleration Effects 0.000 description 1
- 210000001643 allantois Anatomy 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- NEKNNCABDXGBEN-UHFFFAOYSA-L disodium;4-(4-chloro-2-methylphenoxy)butanoate;4-(2,4-dichlorophenoxy)butanoate Chemical compound [Na+].[Na+].CC1=CC(Cl)=CC=C1OCCCC([O-])=O.[O-]C(=O)CCCOC1=CC=C(Cl)C=C1Cl NEKNNCABDXGBEN-UHFFFAOYSA-L 0.000 description 1
- 230000002500 effect on skin Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003195 fascia Anatomy 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 210000000630 fibrocyte Anatomy 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 210000001626 skin fibroblast Anatomy 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/02—Peptides of undefined number of amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/047—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates having two or more hydroxy groups, e.g. sorbitol
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/185—Acids; Anhydrides, halides or salts thereof, e.g. sulfur acids, imidic, hydrazonic or hydroximic acids
- A61K31/19—Carboxylic acids, e.g. valproic acid
- A61K31/195—Carboxylic acids, e.g. valproic acid having an amino group
- A61K31/197—Carboxylic acids, e.g. valproic acid having an amino group the amino and the carboxyl groups being attached to the same acyclic carbon chain, e.g. gamma-aminobutyric acid [GABA], beta-alanine, epsilon-aminocaproic acid or pantothenic acid
- A61K31/198—Alpha-amino acids, e.g. alanine or edetic acid [EDTA]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/335—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin
- A61K31/35—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom
- A61K31/352—Heterocyclic compounds having oxygen as the only ring hetero atom, e.g. fungichromin having six-membered rings with one oxygen as the only ring hetero atom condensed with carbocyclic rings, e.g. methantheline
- A61K31/353—3,4-Dihydrobenzopyrans, e.g. chroman, catechin
- A61K31/355—Tocopherols, e.g. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/33—Heterocyclic compounds
- A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
- A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
- A61K31/4164—1,3-Diazoles
- A61K31/4166—1,3-Diazoles having oxo groups directly attached to the heterocyclic ring, e.g. phenytoin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0014—Skin, i.e. galenical aspects of topical compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/12—Aerosols; Foams
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Dispersion Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of products that can promote wound healing, it is proposed that soybean protein can apply to the effect in terms of promotion human skin wound healing, and soy protein concentration is 0.01% ~ 1.0% in the present invention.The present invention can promote the migration of human epidermal cell, fibroblastic proliferation and human epidermal cell, and so as to accelerate the healing of application on human skin superficial cut, and soybean protein is without any side effects to human body.Present invention relates particularly to a kind of spraying of soybean protein, using water as solvent, including ingredients such as glycerine, EDETATE SODIUM, vitamin E, allantoins.Soybean protein spraying can be preferably permeated in wound made of the present invention, promote wound healing.
Description
Technical field
The invention belongs to biomedicine fields, and in particular to one kind can promote wound healing products.
Background technology
China is the big country of a soybean planting and consumption, and up to 38% protein is contained in soybean, is cereal
4~5 times, this provides abundant raw material sources for further develop and use of soybean protein, and protein structure and function are ground
The development for studying carefully technology and modification techniques of protein also provides technology for soybean protein functionalization and its high value added product exploitation
Ensure.
People inevitably can accidentally cause the scuffing of human body surface skin in daily routines and work.In human body certainly
In my wound repair mechanisms, mainly by the repair ability of human body autologous tissue, but its self-regeneration speed is slow, influences ours
Normal life.Nowadays it is all chemical extraction agent that market circulation, which promotes the product of human body surface wound healing, right to some extent
Human body has certain injury.
Invention content
The present invention relates to a kind of products that can promote wound healing, and where it is proposed soybean proteins to promote human body surface wound
The application of mouth healing.
A kind of product that can promote wound healing of the present invention, it is excellent including the soybean protein that content is 0.01% ~ 1%
It is selected as 0.7%.
A kind of product that can promote wound healing of the present invention, using soybean protein as the main base promoted in wound healing
Bottom additionally includes：Glycerol content is 3 ~ 27%, and the content of EDETATE SODIUM is 0.05 ~ 0.2%, the content of vitamin E for 0.1 ~
1%, the content of allantoin is 0.01 ~ 0.2%, and surplus is deionized water.
Preferably, it is 8% to further include glycerol content, and the content of EDETATE SODIUM is 1%, and the content of vitamin E is 0.5%, allantois
The content of element is 0.1%, and surplus is deionized water.
The invention also discloses the preparation methods of the product, and first, each component is mixed in proportion；Then, will
Mixture ultrasonic activation, three times, vibration 20 minutes, each vibration frequency are 40KHz every time, and power is for continuous shaking
400W makes its abundant mixing；Mixture is pressed into high-pressure aerial fog flask for medicinal preparations, sealing packaging.
The present invention carries out the experiment of application on human skin horn cell according to the situation of human body wound healing, fibroblast is tested and small
A kind of product for the soybean protein spraying for promoting human body wound healing is studied and produced to mouse superficial cut Healing Experiments.It is real
It tests the result shows that soybean protein contributes to skin surface wound healing, promotes the proliferation of human epidermal cell and fibrocyte and move
It moves, accelerates the healing of human body surface wound.
Description of the drawings
Fig. 1 is the absorbance value average value of the embodiment of the present invention 2；
Fig. 2 is the absorbance value average value of the embodiment of the present invention 4.
Specific embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail, and of the invention protects content not limit to
In following embodiment.
1 zoopery of embodiment
Experiment mice 48（C57BL/6 mouse are purchased from Nanjing University of Traditional Chinese Medicine's Animal Research Center）, 220 ~ 280g of weight.It will be small
Mouse divides 8 groups, every group 6 at random：A：Blank control group；B：0.01% soybean protein experimental group；C：0.05% soybean protein experimental group；
D：0.1% soybean protein experimental group；E：0.3% soybean protein experimental group；F：0.5% soybean protein experimental group；G：0.7% soybean protein
Experimental group；H：1.0% soybean protein experimental group.
It is anaesthetized with 0.3% yellow Jackets 1mL/100g intraperitoneal injections, mouse back is lost hair or feathers with vulcanized sodium, uses diameter
After showing boundary for 1.0cm self-control seals, it can not be licked using mouse and lick wound as principle, cut with the card punch of diameter 1.0cm diameters
Except a round full thickness skin, as deep as the wound of deep fascia.Wound is dry and retraction measures wound area after 2 hours, as former wound
Open area.Mouse wound stops blooding naturally, and daily medication is primary and in regular time, until wound healing.Control group painting is erased
150 μ L of ionized water, the daily wound of soybean protein experimental group, which applies, contains 0.01%, 0.05%, 0.1%, 0.3%, 0.5%, 0.7%,
The 150 μ L of solvent of 1.0% soybean protein.From the daystart of experiment process, wound size size and wound face are recorded daily
Product, shown in table 1 specific as follows.
The measurement result of 1 each group mouse compromised skin average diameter of table（cm）
Note：Compared with the control group, *P<0.05,** P<0.01
The experimental results showed that：For control group compared with soybean protein experimental group, soybean protein experimental mice is obviously promoted wound
The effect of healing, third day after the test, soybean protein experiment D groups have significant facilitation with blank control group.From reality
It tests result and can also be seen that there is apparent difference, and the soybean protein of different content between experimental group and control group the 7th day
There is certain difference to mouse skin wound healing.It can be obtained from experimental group data, with the increasing of soybean protein content
Add, facilitation also certain raising.G groups and experiment H groups are tested, healing effect is best in experimental group, and the two difference is not
Significantly.
2 soybean protein of embodiment promotes human epidermal cell proliferation experiment
Using 96 orifice plate culture HaCaT cells, it is separately added into the soybean protein of various concentration（0.01%, 0.05%, 0.1%, 0.3%,
0.5%, 0.7%, 1.0%）, blank control group adds in same amount of culture solution, and after processing for 24 hours, cell Proliferation is detected using mtt assay
It is as shown in Figure 1 to measure absorbance value average value for activity.The experimental results showed that soybean protein, which has, promotes human epidermal cell proliferation
Effect has an apparent facilitation when soybean protein content is 0.3%, and with the significant difference of control group（P<0.05）.Greatly
When legumin content is 0.7% and 1.0%, function and effect do not have notable difference.Under the conditions of function and effect do not have notable difference, take
The few experimental group soybean protein 0.7% of dosage is best experimental group.
3 soybean protein of embodiment promotes human epidermal cell migration experiment
Using 12 orifice plate culture HaCaT cells, at 37 DEG C, 5%CO2Under the conditions of cultivate for 24 hours, human epidermal cell is covered in culture dish
Afterwards, one of cut is gently scratched in culture dish bottom cellular layer with 10 μ L pipette tips, bottom culture solution is sucked out, add in 500 μ L
PBS is rinsed twice, this rinses out floating cells in the process.Control group adds in blank cultures, and experimental group is then added in containing 0.7%
The culture medium of soybean protein.Record the cell growth state after control group and experimental group original state and 12h respectively with microscope,
And check analysis is carried out to it.The experimental results showed that with soybean protein handle human epidermal cell after 12h compared with the control group
Cell migration has apparent acceleration.
4 soybean protein of embodiment promotes proliferation of human dermal fibroblasts experiment
Using human skin fibroblasts 2522 are cultivated in 96 orifice plates, the soybean protein 0.01% of different content is separately added into,
0.05%, 0.1%, 0.3%, 0.5%, 0.7%, after 1.0% processing for 24 hours, cell-proliferation activity is detected using mtt assay, measures absorbance
Average value is as shown in Figure 2.The experimental results showed that soybean protein has the function of to promote fibroblast proliferation, and from soybean protein
When content is 0.3%, there is apparent facilitation to cell growth, soybean protein content 0.7% is real with soybean protein content 1.0%
Test facilitation effect difference unobvious between group.Under the conditions of function and effect do not have notable difference, the few experimental group soybean of the amount of taking
Albumen 0.7% is best experimental group.
5 soybean protein of embodiment is to the effect assessment of human skin wound healing
Product of the present invention is prepared into spraying, take nanometer level soybean protein particle, glycerine, EDETATE SODIUM, vitamin E content,
Allantoin, surplus are water, and by mixture ultrasonic activation, three times, vibration 20 minutes, are sufficiently stirred every time for continuous shaking
Even, press-in has in high-pressure aerial fog flask for medicinal preparations, sealing packaging, you can.
Four groups of sprayings, one group of medicinal spraying for not soybean protein-containing, per 100mL containing 8% glycerine, 1%EDTA is respectively configured
Disodium, 0.5% vitamin E, 0.1% allantoin, 90.4% deionized water.Two groups are sprayed for 0.01% soybean protein, are contained per 100mL
0.01% nanometer level soybean protein, 3% glycerine, 0.05%EDTA disodiums, 0.1% vitamin E, 0.01% allantoin, 96.83% go from
Sub- water.Three groups are sprayed for 0.7% soybean protein, per 100mL containing 0.7% nanometer level soybean protein, 8% glycerine, 1%EDTA bis-
Sodium, 0.5% vitamin E, 0.1% allantoin, 89.7% deionized water.Four groups are sprayed for 1.0% soybean protein, are contained per 100mL
1.0% nanometer level soybean protein, 27% glycerine, 0.2%EDTA disodiums, 1% vitamin E, 0.2% allantoin, 70.6% deionized water.
It selects 40 people with skin wound at random from volunteer, is divided into 4 groups, every group of 10 people.Above-mentioned spray is used respectively
Mist handles wound, twice daily.After 7 days by a definite date, volunteer's feedback, soybean protein spraying can directly act on skin surface,
It is non-stimulated to skin surface, there is facilitation for the wound healing of human body surface.Specific phenomenon shows to contain in soybean protein
It measures and promotes when being 0.7% wound healing effect more notable, preferably best soybean protein optimum content.
Claims (5)
1. a kind of product that can promote wound healing, which is characterized in that contain 0.01% ~ 1% soybean protein.
2. a kind of product that can promote wound healing according to claims 1, which is characterized in that by following component group
Into：Soybean protein granule content is 0.01% ~ 1%, and glycerol content is 3 ~ 27%, and the content of EDETATE SODIUM is 0.05 ~ 0.2%, dimension life
The content of plain E is 0.1 ~ 1%, and the content of allantoin is 0.01 ~ 0.2%, and surplus is deionized water.
3. a kind of product that can promote wound healing according to claims 2, which is characterized in that by following component group
Into：Soybean protein granule content is 0.7%, glycerol content 8%, and the content of EDETATE SODIUM is 1%, and the content of vitamin E is
0.5%, the content of allantoin is 0.1%, and surplus is deionized water.
A kind of 4. product that can promote wound healing according to claims 2, which is characterized in that the soybean egg
White particle is nanoscale.
A kind of 5. preparation method of the product that can promote wound healing described in claims 2 or 3, which is characterized in that packet
Include following steps：First choice mixes in proportion each component；Then, by mixture ultrasonic activation, continuous shaking three
Secondary, vibration 20 minutes, each vibration frequency are 40KHz, power 400W every time, make its abundant mixing；Mixture is pressed into high
It calms the anger in mist flask for medicinal preparations, sealing packaging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810217715.0A CN108187026A (en) | 2018-03-16 | 2018-03-16 | A kind of product that can promote wound healing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810217715.0A CN108187026A (en) | 2018-03-16 | 2018-03-16 | A kind of product that can promote wound healing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108187026A true CN108187026A (en) | 2018-06-22 |
Family
ID=62595398
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810217715.0A Pending CN108187026A (en) | 2018-03-16 | 2018-03-16 | A kind of product that can promote wound healing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108187026A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114470301A (en) * | 2021-12-22 | 2022-05-13 | 南京财经大学 | Preparation method of soybean polypeptide absorbable medical suture line |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110074116A (en) * | 2009-12-24 | 2011-06-30 | 애경산업(주) | Composition for wound healing and method for preparation thereof |
| CN104740677A (en) * | 2015-04-23 | 2015-07-01 | 广西达庆生物科技有限公司 | Medical repairing surgical dressing for skins and preparation method thereof |
| US9248160B1 (en) * | 2015-07-28 | 2016-02-02 | Zo Skin Health, Inc. | Post-procedure skin care systems, compositions, and methods of use thereof |
| CN105944139A (en) * | 2016-07-05 | 2016-09-21 | 四川大学 | Soybean protein isolate-based biomedical adhesive and preparation method thereof |
| CN106176837A (en) * | 2016-08-15 | 2016-12-07 | 江苏红豆杉药业有限公司 | A kind of pharmaceutical composition, its preparation method and application |
-
2018
- 2018-03-16 CN CN201810217715.0A patent/CN108187026A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20110074116A (en) * | 2009-12-24 | 2011-06-30 | 애경산업(주) | Composition for wound healing and method for preparation thereof |
| CN104740677A (en) * | 2015-04-23 | 2015-07-01 | 广西达庆生物科技有限公司 | Medical repairing surgical dressing for skins and preparation method thereof |
| US9248160B1 (en) * | 2015-07-28 | 2016-02-02 | Zo Skin Health, Inc. | Post-procedure skin care systems, compositions, and methods of use thereof |
| CN105944139A (en) * | 2016-07-05 | 2016-09-21 | 四川大学 | Soybean protein isolate-based biomedical adhesive and preparation method thereof |
| CN106176837A (en) * | 2016-08-15 | 2016-12-07 | 江苏红豆杉药业有限公司 | A kind of pharmaceutical composition, its preparation method and application |
Non-Patent Citations (3)
| Title |
|---|
| MATTEO SANTIN ET AL.: ""Soybean-based biomaterials:preparation,properties and tissue regeneration potential"", 《EXPERT REV.MED.DEVICES》 * |
| 刘杰等: ""大豆分离蛋白/琼脂糖复合水凝胶"", 《高等学校化学学报》 * |
| 表面活性剂和洗涤剂行业生产力促进中心: "《中国表面活性剂行业年鉴》", 31 October 2017, 中国轻工业出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114470301A (en) * | 2021-12-22 | 2022-05-13 | 南京财经大学 | Preparation method of soybean polypeptide absorbable medical suture line |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2021045159A (en) | Engineered three-dimensional skin tissues, arrays thereof, and methods of making the same | |
| Mohammadi et al. | Skin diseases modeling using combined tissue engineering and microfluidic technologies | |
| Unanue et al. | Regulation of immunity and inflammation by mediators from macrophages. | |
| CN103845272B (en) | Earth-worm extractive as raw material with resisting age of skin effect and its preparation method and application | |
| Vinante et al. | Heparin-binding epidermal growth factor-like growth factor/diphtheria toxin receptor in normal and neoplastic hematopoiesis | |
| CN107567494A (en) | The cell culture processes of mescenchymal stem cell | |
| JP2009519971A5 (en) | ||
| CN105078777A (en) | Mesenchymal stem cell excreted factor essence, and preparation method and application thereof | |
| CN103393585B (en) | Fibroblast liquid for beauty treatment and preparation method thereof | |
| CN105228590A (en) | To comprise the functional cosmetic composition that somatomedin and aminoacid are feature | |
| KR101800681B1 (en) | Composition for hair care treatment | |
| TW201502832A (en) | Method of obtaining stem cells and their data | |
| CN105722974A (en) | Method for inducing pluripotent stem cells and pluripotent stem cells prepared by said method | |
| CN104736135B (en) | Cosmetic composition | |
| CN100393300C (en) | Nanometer corpuscle comprising fatsoluble vitamin C and di palm hydroxyproline and its cosmetic composition | |
| CN108187026A (en) | A kind of product that can promote wound healing | |
| CN106568911A (en) | In vitro simulated skin model | |
| CN110526957B (en) | Cell permeation enhancing peptides, compositions and uses thereof | |
| CN110368347A (en) | Anti-ageing reparation double-layer bi-color essence of one kind and preparation method thereof | |
| Ayaz et al. | First report of Nitzschia navis-varingica in the Mediterranean Sea and growth stimulatory effects of Nitzschia navis-varingica, Chrysochromulina alifera and Heterocapsa pygmaea on different mammalian cell types | |
| CN110613628B (en) | Anti-aging composition, freeze-dried powder containing anti-aging composition and preparation method of freeze-dried powder | |
| Lee et al. | Hair growth promoting effect of 4HGF encapsulated with PGA nanoparticles (PGA-4HGF) by β-catenin activation and its related cell cycle molecules | |
| Hwang et al. | Collagen hydrolysate from the scales of Mozambique tilapia (Oreochromis mossambicus) improve hair and skin health by alleviating oxidative stress and inflammation and promoting hair growth and extracellular matrix factors | |
| WO2016194760A1 (en) | Cosmetic method and dermatological topical agent for use therein, migration imparting agent, and method for screening components for use in cosmetic method for improving skin state | |
| CN108794574B (en) | Antioxidant active peptide and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180622 |