CN108186456A - A kind of preparation method of freckle removing and whitening emblic extract - Google Patents
A kind of preparation method of freckle removing and whitening emblic extract Download PDFInfo
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- CN108186456A CN108186456A CN201810191017.8A CN201810191017A CN108186456A CN 108186456 A CN108186456 A CN 108186456A CN 201810191017 A CN201810191017 A CN 201810191017A CN 108186456 A CN108186456 A CN 108186456A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/84—Products or compounds obtained by lyophilisation, freeze-drying
Abstract
The present invention provides a kind of preparation methods of the emblic extract with freckle removing and whitening activity.Using emblic fruit as raw material, extracted, filtered by low temperature, the concentration of extracting solution low temperature, separation of solid and liquid, purification with macroreticular resin, eluent concentration, low temperature drying, obtain emblic extract.After testing, for the extraction chemical constituent based on total tannin, impurity component general flavone content is less than 5%.Through melanin inhibit test evaluation, the extract freckle removing and whitening activity significantly, without skin irritation.
Description
Technical field
The invention belongs to cosmetic fields, are related to the preparation method of emblic extract and with the remaining of freckle removing and whitening activity
The preparation method of sweet seed extract.
Background technology
Emblic (Phyllanthus emblica L.) is Euphorbiaceae (Euphorbiaceae) Leafflower
(Phyllanthus) fruit, alias Yunnan olive, Phyllanthus embical fruit, mountain oil are sweet, emblic leafflower fruit, Niuganguo, pyhllanth usemhlica L., poplar are sweet, Hui Ganzi
Deng.Emblic originates in India, Thailand, Philippine, Malaysia, Pakistan etc., maximum with the yield of India and China.It is remaining
Sweet son is distributed mainly on the provinces and regions such as Yunnan, Guangxi, Guangdong, Fujian, Guizhou, Sichuan, Taiwan, Hainan in China, with Yunnan, Fujian
Yield is maximum.Emblic light is shone, can be drought-resistant well, therefore, is had very well to the torrid zone, subtropical zone dry-hot valley ecological environment
Adaptability.Column condition, natural community's large area of emblic are preferably met in China adaptability ofgrowth area
It is distributed in this.The root system of plant is flourishing, and sand prevention is solid native well for energy, and to preventing erosion, improving the ecological environment, there have to be positive
Effect.Therefore, emblic is resourceful, and growth or cultivation are conducive to ecological environment, suitable for developing and using.
Emblic Clinical practice is with a long history and safety is good.Record in《Chinese Pharmacopoeia》, belong to Tibetan and commonly use medicine, have
The effect of " clearing heat and cooling blood, food digesting stomach fortifying, cough-relieving of promoting the production of body fluid ", " removes, indigestion, abdominal distension , gai cough, laryngalgia, mouth for blood-head blood
It is dry " etc. illnesss.Emblic medicine-food two-purpose records what is announced within 2002 in the Ministry of Public Health《It is both food and the item name of drug
It is single》, in the civil custom for having and eating fresh fruit of fructus phyllanthi raw.
Modern plants chemical research shows that emblic mainly contains following chemical composition：Polyphenols, organic acid, carbohydrate,
The substances such as vitamin, amino acid and trace elements zn, Mn, Fe, Ca and Se etc., wherein, it is noteworthy characterized by containing a large amount of
Polyphenol and abundant vitamin C.Polyphenols mainly has：Glucose alkaloid (glucogallin), gallic acid
(gallic acid), benzoaric acid (e11agic acid), corilagin (corlagin), terchebin
(terchebin), chebulinic acid (chebulinc acid), 3,6- digalloylglucose glycosides (3,6-
Digalloylglucose), 3- ethyls gallic acid (3-ethoxy-4,5-dihydroxy-bezoic acid), glactaric acid
(mucic acid) and phyllemblic acid (phyllemblic acid) etc..It is anti-that modern pharmacology research shows that emblic mainly has
The effects that oxidation, resisting pathogenic microbes, anti-mutagenesis, teratogenesis, antitumor, lipid-loweringing, blood pressure lowering.
At present, having foreign patent report phyllemblic tannin constituents has preferable freckle removing and whitening activity.China's emblic
It is resourceful, and the plant medicine-food two-purpose, it is safe and non-toxic, provide good condition for freckle removing and whitening class product development.
Invention content
The object of the present invention is to provide it is a kind of can industrialized production, there is the emblic extract of freckle removing and whitening activity
Preparation method, emblic extract active ingredient total tannin content height, the impurity component general flavone content of gained are low, appearance color
It is shallow, it is safe and non-toxic, it is well positioned to meet the market demand of freckle removing and whitening.
To achieve the above object, the present invention provides a kind of preparation sides of the emblic extraction with freckle removing and whitening activity
Method, the step of progress including order below：
A kind of preparation method of freckle removing and whitening emblic extract, which is characterized in that the system of the emblic extract
Preparation Method includes the following steps：
Emblic fruit, low temperature extraction, filtering, the concentration of filtrate low temperature, concentrate are dissolved in water, and separation of solid and liquid clarifies aqueous
Upper purification with macroreticular resin, elutes through different concentration ethanol, collects the ethanol eluate of specific alcoholic degree, concentrates, dry, detection
Qualification, packaging, obtains emblic extract.
The preparation method of a kind of freckle removing and whitening emblic extract, which is characterized in that low temperature extraction process includes：
1. extracting mode is emblic fruit, crushes or do not crush, low temperature extraction, especially with cold soaking, temperature leaching, ultrasonic one kind or number
The combination of kind method extracts；2. extraction solvent is acetone, the arbitrary composition of one or several kinds of solvents of methanol, ethyl alcohol, water
The aqueous acetone solution of ratio, particularly 40%~70%, 40%~70% ethanol water, 40%~70% methanol are water-soluble
Liquid；3. solvent dosage is 3~100 times/time of emblic weight, particularly the 5~30 of emblic weight times；4. extraction time is
0.5~for 24 hours/secondary；5. extraction time is 1~5 time, particularly 1~3 time.
The preparation method of a kind of freckle removing and whitening emblic extract, which is characterized in that low-temperature concentration process includes：
1. thickening temperature is 30~70 DEG C, particularly 40~60 DEG C；2. condensing mode is the equipment of low temperature concentration, to extract liquid dense
The equipment that contracting, particularly falling film concentration, micro-filtration membrane concentration, ultrafiltration membrane concentration, NF membrane concentration, reverse osmosis membrane concentrate；3. it concentrates
Terminal is be concentrated into organic solvent-free or volume about emblic weight 1~3 times.
The preparation method of a kind of freckle removing and whitening emblic extract, which is characterized in that solid-liquid separation process includes：
Concentrate adds purified water, and to 2~5 times of emblic weight, mixing makes the precipitation in concentrate be completely dissolved in water, Ran Houjin
Row separation of solid and liquid operate, centrifuged using centrifugation apparatus, plate-frame filtering, micro-filtration film device filtering, filter-cloth filtering mode, particularly
Centrifugation apparatus centrifuges, and collects the aqueous of no precipitation.
The preparation method of a kind of freckle removing and whitening emblic extract, which is characterized in that purifying process includes：It is 1. big
Macroporous adsorbent resin type：Using the macroporous absorbent resin of big polarity or medium and small polarity, as HPD826, LS305, HPD100, LX~
68M, JKSB-1 etc.；2. macroporous absorbent resin pre-treatment：It is clean using high concentration ethanol or through processing such as alkali, acid, ethyl alcohol；On 3.
Sample amount：Applied sample amount is 0.1~5 times of obtained clarification aqueous of (w/v) emblic of macroporous absorbent resin；4. it elutes：After loading
Large pore resin absorption column, purified different concentration ethanol elution successively collects 10%~80% ethanol eluate, particularly
10%~40% ethanol eluate.
The preparation method of a kind of freckle removing and whitening emblic extract, which is characterized in that ethanol eluate concentrates work
Skill includes：1. the ethanol eluate low temperature concentration of upper prop elution gained；2. thickening temperature：To prevent high temperature extracting solution chemical
Ingredient, low temperature range are 10~70 DEG C, particularly 20~60 DEG C；3. condensing mode：The equipment concentrated using energy low temperature is carried out
Extracting solution concentrates, and particularly falling film concentration, micro-filtration membrane concentration, ultrafiltration membrane concentration, NF membrane concentration, reverse osmosis membrane concentration etc. is set
It is standby；4. striking point：It is concentrated into relative density 1.0~2.0, particularly 1.05~1.20.
The preparation method of a kind of freckle removing and whitening emblic extract, which is characterized in that drying process is using micro-
Wave drying, the combination one or several kinds of drying modes such as vacuum freeze drying, be dried under reduced pressure, be spray-dried carry out the dry of concentrate
Dry, the combination especially with one or several kinds of modes such as vacuum freeze drying, spray drying, microwave drying carries out concentrate
It is dry.
The preparation method of a kind of freckle removing and whitening emblic extract, which is characterized in that emblic fruit and liquid are straight
The water of contact is deionized water or purified water.
The preparation method of a kind of freckle removing and whitening emblic extract, which is characterized in that emblic extract is most
End form formula is powder or dry cream.
A kind of preparation method of freckle removing and whitening emblic extract, which is characterized in that the total tan of emblic extract
Matter content is more than 30%, and particularly 40~80%, general flavone content is less than 5%, especially less than 1%.
The preparation method of a kind of freckle removing and whitening emblic extract, which is characterized in that emblic extract has
The effect of freckle removing and whitening and without skin irritation, skin lightening, whitening or the makeup of nti-freckle can be used in as main active
In product.
The present invention has the following advantages：
(1) stable processing technique, feasible, is suitable for industrialized large-scaled production.
(2) the emblic extract freckle removing and whitening activity prepared by above-mentioned technique is notable.
(3) product is originated from the emblic fruit of medicine-food two-purpose, is not added with any exogenous components, safe to use.
(4) quality standard is scientific and reasonable, to having effective control in effect ingredient (total tannin), impurity component (general flavone)
And detection.
(5) production technology is advanced, has used and has been concentrated and dried method suitable for the extraction of heat sensitive material, such as ultrasonic wave or microwave
Extraction, film concentration, falling film concentration, vacuum freeze drying, microwave drying, spray drying etc..
Description of the drawings：
Fig. 1 emblic extraction production technology general flow charts
Specific embodiment
The present invention is further described with reference to example.Although above-mentioned elaborate the present invention, this hair
Bright example without being limited thereto, those skilled in the art can be modified with principle according to the present invention, therefore, all according to this
The various modifications that the principle of invention carries out all should be understood to fall into protection scope of the present invention.
Embodiment 1
Fresh fruit of fructus phyllanthi 100kg, drinking water are cleaned, coarse crushing, add extraction 1 time in 40% EtOH Sonicate extraction equipment, extraction
Time 120min, 40% ethanol consumption are 30 times of amounts (V/W), and the filtration of 100 mesh filter cloth of extracting solution, filtrate is in falling film concentration plants
In, 60 DEG C are concentrated under reduced pressure into no alcohol, and concentrate adds purified water to be dissolved to 3 times of amounts of emblic weight, tube centrifuge centrifugation, from
Heart liquid is added on the LX-68M macroporous absorption columns balanced through soda acid, 95% alcohol treatment and purified water that (emblic weight is big
Hole absorption 2 times of column volume), upper column flow rate is 1BV/h, then purified water, 10% ethyl alcohol, each 3BV of 80% ethanol elution with
1BV/h flow velocitys elute, and collect 80% ethanol eluate, and 60 DEG C of decompressions, which are protected from light, in falling film condenser is concentrated into 1.10 (40 DEG C),
Concentrate is spray-dried (inlet air temperature：170 DEG C, it is atomized frequency：300Hz, leaving air temp：100~110 DEG C), it obtains emblic and carries
Object dried cream powder is taken, density polyethylene bag is set low and is packaged into 500g/ bags, then puts shady and cool, dry, ventilation and preserves.The total tan of dried cream powder
Matter content 75.3%, general flavone content 4.5%.
Embodiment 2
Emblic dry fruit 800kg, coarse crushing add 70% acetone to take 2 times in 50 DEG C of temperature extractions in refluxing extraction tank, every time
60min, every time 15 times of amounts (V/W), the filtration of 100 mesh filter cloth of extracting solution, in falling film concentration plants, 40 DEG C are concentrated under reduced pressure into filtrate
Acetone, concentrate add purified water to be dissolved to 2 times of emblic weight amounts, and 200 mesh filter-cloth filterings, filtrate is added in through at 95% ethyl alcohol
(emblic weight is 1 times of macroporous absorption column volume), upper column flow rate on the LS305 macroporous absorption columns that reason and purified water have balanced
For 1.5BV/h, then eluted through 20% ethyl alcohol, each 3BV of 70% ethanol elution with 1BV/h flow velocitys, collect 70% ethanol eluate,
It is protected from light in the 60 DEG C of decompressions of economic benefits and social benefits contracting device and is concentrated into 1.20 (50 DEG C), concentrate vacuum freeze drying (pre-freezing temperature：40 DEG C, pre-freeze
Time：60min；Then it is stepped up temperature to distil, obtains emblic extract dry cream, crush, sieve with 100 mesh sieve, get dry extract
Powder sets low density polyethylene bag and is packaged into 5kg/ bags, then puts shady and cool, dry, ventilation and preserve.Dried cream powder total tannin content
55.9%%, general flavone content 1.5%.
Embodiment 3
Emblic dry fruit 700kg, coarse crushing, add 50% methanol in extractor cold soaking extract 3 times, every time for 24 hours, 20 times every time
It measures (V/W), extracting solution filtration, for filtrate in dual-effect concentrator, 50 DEG C are concentrated under reduced pressure into no methanol (alcohol meter measure condensate liquid meter
Number is 0), concentrate adds purified water to be dissolved to 5 times of amounts of emblic weight, and centrifugation, centrifugate, which is added in, to be handled and purified through soda acid
On the good HPD826 macroporous absorption columns of water balance (emblic weight is 0.5 times of macroporous absorption column volume), upper column flow rate is
Then 0.5BV/h is eluted with 1BV/h flow velocitys through 20%, 50% each 3BV of ethanol elution, 50% ethanol eluate is collected, in reverse osmosis
1.20 (20 DEG C) are reduced in permeable membrane, 50 DEG C of vacuum decompression dryings of concentrate obtain emblic extract dry cream, crush, cross 200 mesh
Sieve, get dry extract powder, puts Brown Glass Brown glass bottles and jars only inner packing into 5kg/ bottles, then puts shady and cool, dry, ventilation and preserve.Dried cream powder total tannin
Content 70.3%, general flavone content 4.1%.
Embodiment 4
Emblic dry fruit 500kg, coarse crushing add purified water ultrasonic extraction 3 times, and each 60min, every time 50 times of amounts (V/W) carry
Liquid is taken to centrifuge, centrifugate is concentrated into 3 times of emblic weight amounts in nano-filtration membrane equipment, be then added in through 95% alcohol treatment and
On the JKSB-1 macroporous absorption columns that purified water has balanced (emblic weight is 1 times of macroporous absorption column volume), upper column flow rate is
Then 1BV/h is eluted with 1BV/h flow velocitys through 10% ethyl alcohol, each 3BV of 40% ethanol elution, collects 40% ethanol eluate, Yu Na
1.10 (25 DEG C) are reduced in filter membrane, concentrate microwave drying obtains emblic extract dry cream, crushes, and crosses 80 mesh sieve, gets dry extract
Powder puts rustless steel container inner packing into 10kg/ bottles, then puts shady and cool, dry, ventilation and preserve.Dried cream powder total tannin content
40.7%, general flavone content 0.9%.
Embodiment 5
Fresh fruit of fructus phyllanthi 1000kg, coarse crushing add in 50% EtOH Sonicate extraction equipment and extract 2 times, extraction time 60min,
40% ethanol consumption is 15 times of amount/time (V/W), and the filtration of 100 mesh filter cloth of extracting solution, in falling film concentration plants, 50 DEG C subtract filtrate
Pressure is concentrated into no alcohol, and concentrate adds purified water to be dissolved to 3 times of amounts of emblic weight, tube centrifuge centrifugation, and centrifugate is added in
(emblic weight is macroporous absorption column on the LX-68M macroporous absorption columns balanced through soda acid, 95% alcohol treatment and purified water
2 times of volume), upper column flow rate is 1BV/h, is then eluted, received with 1BV/h flow velocitys through 10% ethyl alcohol, each 3BV of 50% ethanol elution
Collecting 50% ethanol eluate, 50 DEG C of decompressions, which are protected from light, in falling film condenser is concentrated into 1.15 (40 DEG C), concentrate spray drying (into
Air temperature：170 DEG C, it is atomized frequency：300Hz, leaving air temp：100~110 DEG C), emblic extract dried cream powder is obtained, is set low close
Degree Polythene Bag is packaged into 500g/ bags, then puts shady and cool, dry, ventilation and preserve.Dried cream powder total tannin content 65.3%, it is total yellow
Ketone content 1.2%.
6 freckle removing and whitening activity rating of embodiment (melanin inhibits experiment)
The emblic extract whitening active that this experiment prepares embodiment 5 is evaluated.Operation is as follows：1. cell is trained
It supports：The B16-F10 cells of selection tradition selection secondary culture, digest through 0.25% membrane proteolytic enzyme, with containing 8% fetal calf serum
DMEM high sugar culture solution passages, are placed in 37 DEG C, 5%CO2It is cultivated in the incubator of saturated humidity environment.2d is changed the liquid once,
Experiment is derived from same passage cell each time.2. given the test agent tests the inhibition of melanin in B16~F10 cells：Treat B16-
When F10 cell growths are to 80%~90%, cell dissociation is adjusted into cell concentration into individual cells suspension with 0.25% film enzyme
For 1.0 × 105CellsmL-1.Per hole 2mL, inoculation is planted in 6 well culture plates, and culture plate is placed in 37 DEG C, 5%CO2Incubator
Middle culture is for 24 hours.Reject cell conditioned medium culture solution after for 24 hours, experiment are divided into blank group, vehicle control group, positive controls and emblic
Sub- test sample group.Blank group:3mLDMEM culture solutions are added in per hole；Positive controls and emblic test sample group:Emblic is supplied
Test product and kojic acid are diluted to 1,5,20 μ gmL with DMEM culture solutions-1, emblic test sample is diluted to 0.2 with DMEM culture solutions,
1、5μg·mL-1, 3mL is added in per hole；Vehicle control group:The DMEM culture solutions of 3mL DMSO containing respective volume are added in per hole.Each
Parallel 3 multiple holes of concentration.Supernatant is abandoned after effect B16-F10 cells 48h, cell is cleaned 2 times with PBS, per hole 0.5mL concentration
For 1molL-1NaOH dissolve intracellular melanin, treat that melanin is completely dissolved and absorption photometric value measured after 450nm at.
Melanin relative amount is calculated by following equation.Tested group of mean OD value of melanin content/%=/blank group mean OD value ×
100.3. result：With blank group ratio, positive control (kojic acid) is in 1-20 μ gmL-1The table of melanin in the interior cell to B16-F10
Up to significantly inhibiting, difference has statistical significance (P ＜ 0.01)；Emblic extract is in 0.2-5 μ gmL-1It is right
The expression of melanin significantly inhibits in B16-F10 cells, and difference has statistical significance (P ＜ 0.01).
7 skin irritation test of embodiment
The emblic extract toxicity that this experiment prepares embodiment 5 is evaluated：With reference to the 2007 Nian Ban Ministry of Public Health《Makeup
Product hygienic practice》In skin irritation/corrosion test specification, evaluation emblic extract to rabbit local skin whether spinosity
Swash property and its stimulus intensity.Method：Emblic extract powder end is configured to suspension, is then applied to rabbit intact skin
On, once a day, 14d is continuously smeared, observe the symptoms performance daily.As a result：Emblic extract multiple skin irritation to rabbit
Every animal integral mean value is 0.05 daily for the dermoreaction of experiment, judges emblic extract to family's rabbit skin according to criterion
It is nonirritant.Conclusion：Emblic extract sample is to rabbit no skin irritation.
Claims (11)
- A kind of 1. preparation method of freckle removing and whitening emblic extract, which is characterized in that the preparation of the emblic extract Method includes the following steps：Emblic fruit, low temperature extraction, filtering, the concentration of filtrate low temperature, concentrate are dissolved in water, separation of solid and liquid, Purification with macroreticular resin in aqueous is clarified, is eluted through different concentration ethanol, collects the ethanol eluate of specific alcoholic degree, is concentrated, Dry, detection is qualified, and packaging obtains emblic extract.
- 2. the preparation method of a kind of freckle removing and whitening emblic extract according to claim 1, which is characterized in that low temperature carries Taking technique includes：1. extracting mode is emblic fruit, crushes or do not crush, low temperature extraction especially with cold soaking, temperature leaching, surpasses The combination of one or several kinds of methods of sound extracts；2. extraction solvent is acetone, the one or several kinds of methanol, ethyl alcohol, water are molten The arbitrary composition ratio of matchmaker, particularly 40%~70% aqueous acetone solution, 40%~70% ethanol water, 40%~70% first Alcohol solution；3. solvent dosage is 3~100 times/time of emblic weight, particularly the 5~30 of emblic weight times；4. it extracts Time is 0.5~24 h/ time；5. extraction time is 1~5 time, particularly 1~3 time.
- 3. the preparation method of a kind of freckle removing and whitening emblic extract according to claim 1, which is characterized in that low temperature is dense Contracting technique includes：1. thickening temperature is 30~70 DEG C, particularly 40~60 DEG C；2. condensing mode is the equipment of low temperature concentration It extracts liquid to concentrate, particularly falling film concentration, micro-filtration membrane concentration, ultrafiltration membrane concentration, NF membrane concentration, reverse osmosis membrane concentrate Equipment；3. striking point is be concentrated into organic solvent-free or volume about emblic weight 1~3 times.
- A kind of 4. preparation method of freckle removing and whitening emblic extract according to claim 1, which is characterized in that solid-liquid point Separating process includes：Concentrate adds purified water, and to 2~5 times of emblic weight, mixing is completely dissolved in the precipitation in concentrate In water, then carry out separation of solid and liquid operation, centrifuged using centrifugation apparatus, the filtering of plate-frame filtering, micro-filtration film device, filter-cloth filtering Mode, particularly centrifugation apparatus centrifuge, and collect the aqueous of no precipitation.
- A kind of 5. preparation method of freckle removing and whitening emblic extract according to claim 1, which is characterized in that purifying work Skill includes：1. macroporous absorbent resin type：Using the macroporous absorbent resin of big polarity or medium and small polarity, as HPD826, LS305, HPD100, LX-68M, JKSB-1 etc.；2. macroporous absorbent resin pre-treatment：Using high concentration ethanol or through alkali, acid, ethyl alcohol etc. Reason is clean；3. applied sample amount：Applied sample amount is 0.1~5 times of macroporous absorbent resin（w/v）The obtained clarification aqueous of emblic；④ Elution：Large pore resin absorption column after loading, purified different concentration ethanol elution, collects 10%~80% ethanol elution successively The ethanol eluate of liquid, particularly 10%~40%.
- 6. the preparation method of a kind of freckle removing and whitening emblic extract according to claim 1, which is characterized in that ethyl alcohol is washed De- liquid concentration technique includes：1. the ethanol eluate low temperature concentration of upper prop elution gained；2. thickening temperature：To prevent high temperature from breaking Bad extracting solution chemical composition, low temperature range are 10~70 DEG C, particularly 20~60 DEG C；3. condensing mode：It is dense using energy low temperature The equipment of contracting extracts liquid and concentrates, and particularly falling film concentration, micro-filtration membrane concentration, ultrafiltration membrane concentration, NF membrane concentrate, are reverse osmosis The equipment such as film concentration；4. striking point：It is concentrated into relative density 1.0~2.0, particularly 1.05~1.20.
- A kind of 7. preparation method of freckle removing and whitening emblic extract according to claim 1, which is characterized in that back tender Skill is using microwave drying, the combination progress for vacuum freeze drying, being dried under reduced pressure, one or several kinds of drying modes such as be spray-dried The drying of concentrate, especially with one or several kinds of modes such as vacuum freeze drying, spray drying, microwave drying combination into The drying of row concentrate.
- A kind of 8. preparation method of freckle removing and whitening emblic extract according to claim 1, which is characterized in that emblic The water that fruit and liquid are in direct contact is deionized water or purified water.
- A kind of 9. preparation method of freckle removing and whitening emblic extract according to claim 1, which is characterized in that emblic The final form of extract is powder or dry cream.
- 10. the preparation method of a kind of freckle removing and whitening emblic extract according to claim 1-9, which is characterized in that remaining Sweet seed extract total tannin content is more than 30%, and particularly 40~80%, general flavone content is less than 5%, especially less than 1%.
- 11. the preparation method of a kind of freckle removing and whitening emblic extract according to claim 1-10, which is characterized in that remaining Sweet seed extract has effects that freckle removing and whitening and without skin irritation, main active can be used as to be used in skin lightening, U.S. In the white or cosmetics of nti-freckle.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108935904A (en) * | 2018-09-26 | 2018-12-07 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of processing method of persimmon piece preserved fruit |
| CN109867704A (en) * | 2019-04-21 | 2019-06-11 | 北京中医药大学 | A kind of method that ethyl alcohol temperature leaching method prepares Chebulagic acid |
| CN109876031A (en) * | 2019-04-21 | 2019-06-14 | 北京中医药大学 | A kind of enrichment method at phyllemblic tannin position |
| CN111772227A (en) * | 2020-08-10 | 2020-10-16 | 四川中烟工业有限责任公司 | Phyllanthus emblica targeted refined substance, preparation method and application thereof in cigarettes |
| CN113940430A (en) * | 2021-11-22 | 2022-01-18 | 广东青云山药业有限公司 | Preparation method of emblic leafflower fruit powder |
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