CN108184658B - Method for measuring, matching and picking up spike of new hybrid rice combination - Google Patents
Method for measuring, matching and picking up spike of new hybrid rice combination Download PDFInfo
- Publication number
- CN108184658B CN108184658B CN201810165354.XA CN201810165354A CN108184658B CN 108184658 B CN108184658 B CN 108184658B CN 201810165354 A CN201810165354 A CN 201810165354A CN 108184658 B CN108184658 B CN 108184658B
- Authority
- CN
- China
- Prior art keywords
- ears
- rice
- line
- restorer
- hybridization
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention belongs to the technical field of hybrid rice, and discloses a method for measuring, matching and picking up new combinations of hybrid rice, wherein a processed sterile line is bagged, a pencil is used for marking the sterile line and a restorer line number on the hybrid bag, and a pollination date is marked above the hybrid bag; sequentially taking ears according to the sequence of the restorer marked on the hybridization bag and the sequence number; and soaking the obtained male parent rice ears in field water for wetting, and taking 30 single plant numbers each time. The invention improves the efficiency of measurement and preparation, and the number of the test and preparation can reach 60 to 90 per day; the invention improves the pollination accuracy rate, and the accuracy rate can reach 100%; the rice ears of the invention are convenient to control water: the accuracy and the efficiency of pollination are improved, scientific research personnel provide a technology of clamping the ears by hands, and the efficiency and the accuracy of measurement and matching are greatly improved.
Description
Technical Field
The invention belongs to the technical field of hybrid rice, and particularly relates to a method for measuring, matching and picking up ears of a new hybrid rice combination.
Background
The testing and matching of the new hybrid rice combination is a key link of hybrid rice breeding, and a great amount of new hybrid rice combinations are tested and matched by using a parent sterile line and a restorer line, and target combinations are screened from the new hybrid rice combinations. Due to the restrictive factors such as short flowering phase, the new combination has short measuring time, large workload and high required accuracy. The current technical scheme mainly utilizes page turning to take the ears, and straw caps are also used for taking the ears and the like. Both methods generally require 2 individuals to engage in tassel removal. The page-turning and spike-taking method also needs page-by-page marking, inconvenient water control and the like, and each 1 strain number needs to be separately soaked, so that the page is easy to absorb water and the spike of rice is easy to lose water when the water is less soaked, and the normal flowering is not facilitated; the excessive soaking causes the pages of the book to be wetted, which is not favorable for marking page by page. The straw hat spike picking method has great operational technical risk under the condition of strong wind in the Hainan field and the like, and has the problems of being difficult to clear due to errors and the like.
In summary, the problems of the prior art are as follows: the prior technical scheme has the defects of complex operation procedure, high labor intensity, low matching efficiency and high error rate.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for measuring, matching and picking up ears of a new hybrid rice combination.
The invention is realized in such a way that the method for measuring, matching and picking up the ears of the new hybrid rice combination comprises the following steps:
step one, bagging the treated sterile line, marking the serial numbers of the sterile line and the restorer line on the hybridization bag by a pencil, and marking the pollination date above the hybridization bag;
step two, picking the ears in sequence according to the sequence of the restorer marked on the hybridization bag;
and step three, soaking the selected rice ears of the male parent in field water for wetting, and selecting 30 single-plant numbers each time.
Further, the step one needs to be preceded by:
(1) observing the rice restoring line material according to the heading stage of the sterile line, selecting the number of the line which has undergone heading and flowering, registering on paper, and arranging four numbers in a row;
(2) the sterile line is processed by rice glume cutting or glume cutting-free processing technology before flowering in the afternoon or the morning to promote the exposure of the stigma of the sterile line.
Further, the second step specifically includes: selecting spikes with flowers on the top, shearing 2 spikes of each restorer line strain, and shearing off the flowers on the restorer lines; clamping the 1 st numbered rice ear between the index finger and the middle finger, clamping the second numbered rice ear between the middle finger and the ring finger, clamping the third numbered rice ear between the ring finger and the little finger, and enabling the rice ears to face outwards; the fourth is clamped between the thumb and the forefinger, the rice ears are close to the front, and the stalks and the first three are crossed to form an interlayer; corresponding to a row of four numbers on the paper, the No. 1 is clamped between the index finger and the middle finger, and the No. 4 is transversely arranged and is a layer number; the second layer is the second row number, and the ears are cut in sequence.
Further, the third step specifically includes: before putting the rice ears into the hybridization bags, throwing away redundant water from the rice ears, and putting the single plant numbers into the hybridization bags in sequence according to the reversed sequence of the mark numbers from the last number-taking restoring line; the powder feeding is started after 10 minutes, and the distance is opened and the bag is gently shaken when the powder feeding is carried out on the adjacent hybridization bags.
The invention improves the efficiency of measurement and matching: in the past, page-turning book clamps are generally used, in order to improve the accuracy, each material needs to be registered on each page, two persons are generally matched when ears are taken, one ear of material needs to be wetted once, page turning and registration are carried out, the procedure is complex, the powder taking speed is low, and the efficiency is low. The powder taking by the hand-clamping method greatly improves the ear taking efficiency, and the ear taking speed is 2-3 times of the original ear taking speed. The ear is taken too early or too late, so that the ear does not bloom and loose powder, the workload of each person in the traditional technology is about 30, and the prior art can achieve 60-90 ears per day.
The invention improves the pollination accuracy: the conventional method has high error rate when a plurality of materials are powdered. Often, a material error and a plurality of material errors occur easily, and the error correction difficulty is large. The invention can easily find errors according to the positions of the ears on hands when the ears are taken and placed, and can correct the errors in time, and the accuracy can reach 100 percent only by correct operation.
The rice ears of the invention are convenient to control water: the influence of water on the blooming and the pollen scattering of the ears is great. When the water is less, the ears are dehydrated and dried, so that the ears do not bloom and loose powder, and when the water is more, the normal powder scattering is directly influenced. When the powder is taken by the book end method, the difficulty of water control is high, more water is used, pages can be wetted, and the pencil is inconvenient to register; when water is less, the pages can quickly absorb the water, so that the pages are dehydrated and do not bloom. The hand-clamping method can obviously and effectively overcome the defect, one hand-clamping method is not needed, one-time wetting water can be cut off, and the excess water is thrown away before the hand-clamping method is put into the hybridization bag, so that the operation is convenient and fast. In order to overcome the technical defects of the traditional technology and improve the accuracy and efficiency of pollination, scientific research personnel provide a technology of clamping ears by hands, and the efficiency and accuracy of measurement and matching are greatly improved.
Drawings
FIG. 1 is a flow chart of the method for measuring matched ears of a new combination of hybrid rice provided by the embodiment of the invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
The following detailed description of the principles of the invention is provided in connection with the accompanying drawings.
As shown in FIG. 1, the method for measuring matched ears of a new hybrid rice combination provided by the embodiment of the invention comprises the following steps:
s101: observing the rice restoring line material according to the heading stage of the sterile line, selecting the number of the line which has already taken out ears and bloomed, registering on paper, arranging in a row of four numbers, and conveniently taking the ears according to the number when taking the ears;
s102: the rice glume cutting or glume cutting-free treatment technology is utilized, the sterile line is treated before flowering in the afternoon or the morning, so that the exposure of the stigma of the sterile line is promoted, and the cross pollination is facilitated;
s103: bagging the treated sterile line, marking the serial numbers of the sterile line and the restorer line on the hybridization bag by a pencil, and marking the pollination date above the hybridization bag, so as to be convenient for observing and determining the seed receiving date; sequentially bagging according to the strain number of the restorer line when bagging is carried out, so that ears can be conveniently put into the hybridization bags in sequence;
s104: sequentially taking ears according to the sequence of the restorer marked on the hybridization bag and the sequence number; selecting spikes with flowers on the top, shearing 2 spikes of each restorer line strain, and shearing off the flowers on the restorer lines; the palm is opened with the palm facing upwards, the No. 1 rice ear is clamped between the index finger and the middle finger, the second is clamped between the middle finger and the ring finger, the third is clamped between the ring finger and the little finger, and the rice ear faces outwards; the fourth is clamped between the thumb and the forefinger, the rice ears are close to the front, and the stalks and the first three are crossed to form an interlayer; corresponding to a row of four numbers on the paper, the No. 1 is clamped between the index finger and the middle finger, and the No. 4 is transversely arranged and is a layer number; the second layer is a second row number, and the ears are sequentially cut;
s105: the well-taken rice ears of the male parent are soaked in field water for wetting, about 30 single plant numbers are generally taken each time, and if the weather is high in temperature and low in humidity, the rice ears are soaked in water in advance, so that the influence on the normal flowering of the male parent caused by water loss is avoided. And (3) throwing away excessive water from the rice ears before putting the rice ears into the hybridization bags, and putting the single plant numbers into the hybridization bags in reverse order according to the mark numbers from the last number-taking restorer line. Powder feeding is started after 10 minutes, and distance is opened for shaking lightly when adjacent hybridization bags are fed with powder, so that powder stringing is avoided;
s106: bag sealing: the hybridization bag after powder feeding is sealed, a piece of rice leaves is pulled to be folded on the bag and is clamped well by a clip, so that the rice leaves are prevented from being blown and folded by wind.
The application of the principles of the present invention will now be described in further detail with reference to specific embodiments.
Example 1: the Chongqing season tests and matches Yu 802A, the sterile line 4/1 is sown, the Yu superior series restorer line 3/15 is sown, the sterile line 7/1 starts ear, and 72 Yu superior series restorer lines of the ear picking are selected in 7/3 am and are sequentially registered on paper, wherein 18 rows are provided in 4 rows. The hybridization bags are written, labeled 802A/1, … …, and are labeled sequentially by restorer line number and the pollination period 7/4 is labeled above the hybridization bags. 7/3 ear selection, spike cutting and bagging are carried out on 802A in the afternoon or 7/4 am, ears are taken in 2 times, and 36 ears are taken in each time. Sequentially taking the spikes according to the sequence of the selected restorer line by a hand-clamping method, soaking the rice field with water to wet the whole spikes, throwing away excessive water before throwing the spikes, rapidly putting the spikes from the last in a hybridization bag in reverse sequence, standing for 10 minutes, observing the flowering condition, sequentially throwing powder and sealing the bags.
Example 2: the Chongqing season is characterized in that Yu 805A is measured and matched, a sterile line 4/1 is sown, a Yu superior line restorer line 3/15 is sown, the sterile line 7/3 starts ear, and 64 Yu superior line restorers with ear picking are selected in 7/5 am and are sequentially registered on paper, wherein 16 rows are provided in a row with 4 numbers. The hybridization bags are written, labeled 805A/3, … …, and are labeled sequentially by restorer line number and the pollination period 7/6 is labeled above the hybridization bags. 7/5 selecting the ears of 805A in the afternoon or 7/6 am, cutting the ears of grass, bagging, and picking up the ears in 2 times, 32 ears at each time. Sequentially taking the spikes according to the sequence of the selected restorer line by a hand-clamping method, soaking the rice field with water to wet the whole spikes, throwing away excessive water before throwing the spikes, rapidly putting the spikes from the last in a hybridization bag in reverse sequence, standing for 10 minutes, observing the flowering condition, sequentially throwing powder and sealing the bags.
Example 3: the method comprises the steps of measuring and matching Yu 92S in Hainan autumn breeding, sowing a sterile line 9/1, sowing a Yu superior series restorer line 8/28, starting the ear of the sterile line 11/15, selecting 80 Yu superior series restorers for ear extraction in 11/17 afternoon, and sequentially registering the 80 Yu superior series restorers on paper, wherein 20 rows are provided in 4 rows. The hybridization bags are written, labeled 92S/1, … …, and are labeled sequentially by restorer line number and the pollination period 11/18 is labeled above the hybridization bags. 11/17 selecting ears at 92S in the afternoon or 11/18 am, cutting off flowering parts, bagging, spraying pesticide on the bagged rice ears at 8:30 am to promote the rice ears to avoid cutting and flowering, and taking ears at 2 times, 40 ears each time. Sequentially taking the spikes according to the sequence of the selected restorer line by a hand-clamping method, soaking the rice field with water to wet the whole spikes, throwing away excessive water before throwing the spikes, rapidly putting the spikes from the last in a hybridization bag in reverse sequence, standing for 10 minutes, observing the flowering condition, sequentially throwing powder and sealing the bags.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents and improvements made within the spirit and principle of the present invention are intended to be included within the scope of the present invention.
Claims (1)
1. A method for measuring matched ears of a new hybrid rice combination is characterized by comprising the following steps:
step one, bagging the treated sterile line, marking the serial numbers of the sterile line and the restorer line on the hybridization bag by using a pencil, and marking the pollination date above the hybridization bag;
step two, picking the ears in sequence according to the sequence of the restorer marked on the hybridization bag;
step three, soaking the selected rice ears of the male parent in field water for wetting, and selecting 30 single-plant numbers each time;
the first step needs to be preceded by:
(1) observing the rice restoring line material according to the heading stage of the sterile line, selecting the number of the line which has undergone heading and flowering, registering on paper, and arranging four numbers in a row;
(2) processing the sterile line by utilizing a rice glume cutting or glume cutting-free processing technology before flowering in the afternoon or the morning to promote the exposure of the stigma of the sterile line;
the second step specifically comprises: selecting spikes with flowers on the top, shearing 2 spikes from each restorer line, and shearing off the flowers on the restorer lines; clamping the 1 st numbered rice ear between the index finger and the middle finger, clamping the second numbered rice ear between the middle finger and the ring finger, clamping the third numbered rice ear between the ring finger and the little finger, and enabling the rice ears to face outwards; the fourth is clamped between the thumb and the forefinger, the rice ears are close to the front, and the stalks and the first three are crossed to form an interlayer; corresponding to a row of four numbers on the paper, the No. 1 is clamped between the index finger and the middle finger, and the No. 4 is transversely arranged and is a layer number; the second layer is a second row number, and the ears are sequentially cut;
the third step specifically comprises: before putting the rice ears into the hybridization bags, throwing away redundant water from the rice ears, and putting the single plant numbers into the hybridization bags in sequence according to the reversed sequence of the mark numbers from the last number-taking restoring line; the powder feeding is started after 10 minutes, and the distance is opened and the bag is gently shaken when the powder feeding is carried out on the adjacent hybridization bags.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810165354.XA CN108184658B (en) | 2018-02-28 | 2018-02-28 | Method for measuring, matching and picking up spike of new hybrid rice combination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810165354.XA CN108184658B (en) | 2018-02-28 | 2018-02-28 | Method for measuring, matching and picking up spike of new hybrid rice combination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108184658A CN108184658A (en) | 2018-06-22 |
| CN108184658B true CN108184658B (en) | 2021-04-30 |
Family
ID=62594270
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810165354.XA Active CN108184658B (en) | 2018-02-28 | 2018-02-28 | Method for measuring, matching and picking up spike of new hybrid rice combination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108184658B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001136854A (en) * | 1999-11-18 | 2001-05-22 | Mitsui Chemicals Inc | Method for artificial mating of rice using hot water emasculation method |
| CN103053409A (en) * | 2013-01-17 | 2013-04-24 | 西南科技大学 | Method for improving test-crossing and back-crossing efficiency during breeding process of hybrid rice |
| CN105028187A (en) * | 2015-09-07 | 2015-11-11 | 重庆市农业科学院 | Rice sterile line glume-shearing-free efficient breeding and pollination method |
| CN107333648A (en) * | 2017-09-12 | 2017-11-10 | 玉林市农业科学院 | A kind of test cross method for improving test cross efficiency during breeding of hybridized rice |
-
2018
- 2018-02-28 CN CN201810165354.XA patent/CN108184658B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001136854A (en) * | 1999-11-18 | 2001-05-22 | Mitsui Chemicals Inc | Method for artificial mating of rice using hot water emasculation method |
| CN103053409A (en) * | 2013-01-17 | 2013-04-24 | 西南科技大学 | Method for improving test-crossing and back-crossing efficiency during breeding process of hybrid rice |
| CN105028187A (en) * | 2015-09-07 | 2015-11-11 | 重庆市农业科学院 | Rice sterile line glume-shearing-free efficient breeding and pollination method |
| CN107333648A (en) * | 2017-09-12 | 2017-11-10 | 玉林市农业科学院 | A kind of test cross method for improving test cross efficiency during breeding of hybridized rice |
Non-Patent Citations (1)
| Title |
|---|
| CO2在籼稻组合测配中的应用初探;李小丽等;《农业开发与装备》;20171231(第12期);104 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108184658A (en) | 2018-06-22 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zhao et al. | Mapping quantitative trait loci for heat tolerance at anthesis in rice using chromosomal segment substitution lines | |
| Jonsson et al. | Flowering in a seed orchard of Pinus sylvestris L. | |
| Chauhan et al. | Plants foraged by bees for honey production in northern India: The diverse flora of India and its implications for apiculture | |
| CN103392586A (en) | Breeding method of drought resistant and cold tolerant Saccharum varieties/lines containing Erianthus fulvus Ness consanguinity | |
| Hofmann et al. | Scanning electron microscopy investigations of middle to late Eocene pollen from the Changchang Basin (Hainan Island, South China)–insights into the paleobiogeography and fossil history of Juglans, Fagus, Lagerstroemia, Mortoniodendron, Cornus, Nyssa, Symplocos and some Icacinaceae in SE Asia | |
| Kudo et al. | Relationships between flowering phenology and fruit-set of dwarf shrubs in alpine fellfields in northern Japan: a comparison with a subarctic heathland in northern Sweden | |
| Khadi et al. | Cotton: an introduction | |
| Pedron et al. | Molecular phylogenetics and taxonomic revision of Habenaria section Pentadactylae (Orchidaceae, Orchidinae) | |
| Shukla et al. | Sugarcane varieties identified by AICRP (S) in India | |
| Macaulay et al. | Effects of simulated acid rain on the morphology, phenology and dry biomass of a local variety of maize (Suwan-1) in Southwestern Nigeria | |
| Ye et al. | Marker-assisted pyramiding of QTLs for heat tolerance and escape upgrades heat resilience in rice (Oryza sativa L.) | |
| CN108184658B (en) | Method for measuring, matching and picking up spike of new hybrid rice combination | |
| CN102144538A (en) | Method for breeding high-oil-content rape variety | |
| Ng et al. | Application of tissue culture to tuber crops at IITA | |
| Tamaio et al. | Origin of successive cambia on stem in three species of Menispermaceae | |
| Vila-Viçosa et al. | New Annotated Checklist of the Portuguese Oaks (Quercus L., Fagaceae) | |
| Okeke et al. | Comparative systematic leaf and petiole anatomical studies of the genus Stachytarpheta found in Awka Nigeria | |
| Qin et al. | Aphyllorchis yachangensis (Orchidaceae), a new holomycotrophic orchid from China | |
| CN107593435B (en) | The identification of strong cold-resistant Chinese cabbage type winter rape study on temperature sensitive male sterility mutant and screening technique | |
| Hong et al. | Status of groundnut bacterial wilt research in Vietnam | |
| Robbrecht et al. | Four flowering plant species described from Katanga (Democratic Republic of the Congo) are based on specimens collected in Guangxi, China | |
| CN103704124A (en) | Seed selection method of oryza glaberrima cytoplasmic male sterile line | |
| Mehndi et al. | Analysis of pollen in honey samples in the district of Prayagraj, India | |
| Akshay et al. | DUS characterization of traditional varieties and advanced cultivars of rice using 29 essential characters. | |
| CN112586341B (en) | Preparation method of recovery line japonica rice in late flowering |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |