CN108182859A - A kind of easy insect specimen production method - Google Patents

A kind of easy insect specimen production method Download PDF

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Publication number
CN108182859A
CN108182859A CN201810124196.3A CN201810124196A CN108182859A CN 108182859 A CN108182859 A CN 108182859A CN 201810124196 A CN201810124196 A CN 201810124196A CN 108182859 A CN108182859 A CN 108182859A
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test tube
insect
sample
flame
easy
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CN201810124196.3A
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杨春琳
许秀兰
刘应高
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Sichuan Agricultural University
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Sichuan Agricultural University
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    • GPHYSICS
    • G09EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
    • G09BEDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
    • G09B23/00Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
    • G09B23/36Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for zoology

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  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Mathematical Optimization (AREA)
  • General Health & Medical Sciences (AREA)
  • Algebra (AREA)
  • Computational Mathematics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Mathematical Analysis (AREA)
  • Health & Medical Sciences (AREA)
  • Mathematical Physics (AREA)
  • Pure & Applied Mathematics (AREA)
  • Business, Economics & Management (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Educational Administration (AREA)
  • Educational Technology (AREA)
  • Theoretical Computer Science (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The present invention provides a kind of easy insect specimen production method, the early-stage preparations including S1 insect test tube samples, the firing of S2 insect specimen test tubes, the maintenance and preservation of S3 insect test tube samples;Its high temperature by using alcohol blast burner softens simple glass test tube, recycles vice that test tube is tightened closing, makes simple test tube easy to make.The present invention makes simply, of low cost, easily obtains, and sample is fully sealed, can long-term persistence, Conservation environment is simple, without particular/special requirement, room temperature shady place is stored in, is managed and protected at low cost, once being made, Saving specimen is complete, not easily damaged, convenient transport is shifted, sample is survivable, and production process is easy, simple operation easy to get started is suitble to promote and apply in agriculture and forestry correlation enterprises and institutions, colleges and universities and scientific research institutions, be used more suitable for base agriculture and forestry practitioner.

Description

A kind of easy insect specimen production method
Technical field
The invention belongs to experimental instrument technology more particularly to a kind of easy insect specimen production methods.
Background technology
Insect specimen making is always one of important work of agriculture and forestry practitioner, is particularly engaged in insect genealogical classification The colleges and universities or scientific research institutions of research.At present, common insect preparation of specimen fado is infusion method.
Sample in kind is packed into test tube sample one kind owned by France in infusion method, i.e. test tube with preserving liquid, then test tube is close Envelope, common test tube are mostly glass system test tube.The encapsulating method of test tube selects appropriate cotton using plug envelope or lid envelope Sealings, the sealing parts such as plug, stopper, rubber stopper or pairing bottle cap select the adhesives such as silica gel further to seal, although this method operates It is convenient, but the Saving specimen time do not grow, the easy gas leakage of sealing part.In addition it is sealed individually, but its is complicated for operation, safeguarded using sealing machine It is expensive, it is not easy to promote.
Invention content
It is an object of the invention to solve the problems of the above-mentioned prior art, a kind of easy insect specimen is provided and is made Method is fired various specifications glass system test tube using alcohol blast burner, and sealing effect is good, and manufactured sample can be protected for a long time It deposits, and simple operation, it is of low cost, it is suitable for large-scale promotion with applying.
The present invention adopts the following technical scheme that:
A kind of easy insect specimen production method, in turn includes the following steps:
The early-stage preparations of S1, insect test tube sample
The early-stage preparations of insect test tube include:
(1), sample in kind prepares and handles;
Wherein, the sample in kind preparation includes with processing
Step a, according to the test tube of the form of insect, length selection different model specification;
Step b, the insect specimen made is put into test tube bottom;
Step c, preservation liquid is poured into, liquid level is made to be located at the 1/3 of test tube and is stopped;
Step d, residual liquid more than test tube liquid level is made to be heated repeatedly on alcolhol burner, until residual liquid is evaporated Entirely;
Step e, the test tube in step d is put into rack for test tube, cooling for use;
(2), alcohol blast burner prepares and adjusts;
Step a, alcohol blast burner is taken out, absolute ethyl alcohol step b is fitted into, lights a fire to alcohol blast burner in step a;
Step c, after flame stabilization, the flame of alcohol blast burner is adjusted, its firepower is made to reach maximum;
(3), the adjustment of state before test tube is fired
Step a, it will wait that firing (1) sample in kind prepares and handle, the test tube in step e takes out;
Step b, the test tube in step a is clamped with vice;
Step c, vice is held, by test tube at bottom 1/2~1/3, is placed in flame and is fired;
The firing of S2, insect specimen test tube
(1) heating and softening of test tube;
Step a, test tube is placed in calcination in flame, and rotated repeatedly;
Step b, the test tube in step a is made to continue 1~3min of calcination on flame, makes tube wall homogeneous heating;
Step c, examination is twisted, until test tube can turn easily;
(2) rotation and sintering of test tube;
Step a, certain stretching is carried out while rotation, hand-held test tube bottom end is rotated, and nozzle is fixed at vice end It is stretched, slow stretching, until test tube is closed completely;
Step b, again twist 3~5 circle, stop rotating, calcination point be slowly moved away from flame, while stretch test tube, make top with Lower part completely disengages, until calcination point a bit of fiberglass column formed above, a diameter of 0.2~0.5cm, length for 1~ 1.5cm;
Step c, the glass column in step b is put into flame and carries out calcination, bent and fitted with vice after softening Work as extruding;
(3) cooling and inspection of test tube;
Step a, by the rotation and sintering of (2) test tube, the test tube that is baked in step c is put into rack for test tube and is cooled down, and puts 10~15min is put, test tube is taken out after cooling and is inverted, is made at sintering downward;
Step b, the test tube in up-down vibration step a repeatedly, checks whether leakage;
The maintenance and preservation of S3, insect test tube sample
Step a, it by the firing of S2 insect test tube sample test tubes, the cooling and inspection of (3) test tube, in step b, bakes Test tube sample is put into the Riker mount for containing cotton;
Step b, by the Riker mount in step a be placed in room temperature, drying environment.
S1 (1) of the present invention sample in kind prepares and processing, the test tube specification in step a is 18mm × 180mm, 15mm × 150mm, 12mm × 120mm, tetra- kinds of 10mm × 100mm.
The adjustment of state before S1 (3) test tube of the present invention is fired, vice clip position is apart from test tube in step b At mouth 1/3~1/6, up at 1/6, test tube tilts 30 °~50 ° angles for holding test tube bottom.
S2 alcohol blast burners of the present invention prepare and adjustment, step a, step b, step in the heating and softening of (1) test tube Suddenly step a, step b are operated on flame in the rotation and sintering of c, (2) test tube.
S2 alcohol blast burners of the present invention prepare and adjustment, in the heating and softening of (1) test tube, test tube four in step c Week need to continue rotary heating, be screwed again after its completely softening.
S2 alcohol blast burners of the present invention prepare and adjustment, in the rotation and sintering of (2) test tube, in step a rotation with Stretching is carried out at the same time, and dynamics should not be too large, and when circumgyration stretch makes test tube caliber be less than former caliber 1/2, rotation is with stretching dynamics It can suitably increase, be calcination point rapid closing, form the nearly taper sealing of spiral that is wide and becoming narrow gradually under one.
Spiral number of the present invention is 5~10 circles, and the interval between every spiral coil is unsuitable overstocked, between spiral coil It is spaced 1.5~3mm.
Nearly taper of the present invention seals high 10~15mm.
In the firing of S2 insects test tube sample test tube of the present invention, test tube is placed in calcination on flame, liquid level in step a It needs far from flame, 10~15mm of liquid level positional distance flame location on test tube slant.
Beneficial effects of the present invention:
(1) for the present invention using common test tube as making material, tools are simple, of low cost, easily obtain.
(2) after the present invention is softened test tube using alcohol blast burner, test tube is sealed by vice, realizes insect specimen Be fully sealed, can long-term persistence.
(3) Conservation environment of sample of the present invention is simple, and no particular/special requirement is stored in room temperature shady place.
(4) cost that specimen test tube of the present invention is safeguarded is low, and once being made, Saving specimen is complete, not easily damaged.
(5) sample transfer convenient transport of the present invention, sample are survivable.
(6) production process of the present invention is easy, and simple operation easy to get started is suitble in agriculture and forestry correlation enterprises and institutions, colleges and universities It promotes and applies with scientific research institutions, used more suitable for base agriculture and forestry practitioner.
Description of the drawings
Fig. 1 is fires rear screw enclosuring structure schematic shapes.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, the technical solution below in the present invention carries out clear Chu is fully described by, it is clear that described embodiment is part of the embodiment of the present invention, instead of all the embodiments.It is based on Embodiment in the present invention, those of ordinary skill in the art are obtained every other without creative efforts Embodiment shall fall within the protection scope of the present invention.
As shown in Figure 1:
Embodiment 1
1. the early-stage preparations of insect test tube sample
(1) sample in kind and processing
Different size model is selected according to sample in kind (ovum, larva, the pupa of such as insect, the smaller adult of some bodily forms) Test tube.
Because the flame flame of alcohol blast burner is trapped among 20mm or so, therefore diameter≤18mm of test tube is selected, it is typically chosen 10~ 15mm, diameter is excessive, and it is uneven that tube wall will appear heating.
Wall thickness≤1.5mm of test tube, tube wall is blocked up to lead to rotation and difficulty during stretching, and blocked up at firing calcination point, Calcination point in cooling procedure is easy to cause to crack;The wall thickness of test tube preferably 1.0~1.5mm is advisable, this is because tube wall is excessively thin, examination Tube capacity is easily damaged, it is not easy to preserve.
Diameter 10~13mm test tubes, pipe range are not less than 110mm, 14~16mm of diameter, and pipe range is not less than 140mm, diameter 17 ~18mm, pipe range are not less than 170mm, pipe range longest≤180mm.
Currently used test tube model has 18mm × 180mm, 15mm × 150mm, 12mm × 120mm, 10mm × 100mm tetra- Kind.
The sample in kind of select is put into test tube bottom, pours into preservation liquid, liquid level is at test tube 1/3.
Residual liquid heats repeatedly on alcolhol burner on test tube liquid level, and rack for test tube cooling is put into after making Liquid Residue evaporation completely For use.
(2) alcohol blast burner prepares and adjusts
Alcohol blast burner can be selected according to actual conditions, and existing alcohol blast burner has block-type alcohol blowtorch, hanging alcohol blast burner.
Alcohol blast burner is taken out, is packed into absolute ethyl alcohol, igniting treats that flame stabilization is adjusted to firepower maximum.
Because common test tube is made of simple glass on the market, general temperature can soften at 600 DEG C or so, alcohol spray Lamp maximum temperature selects alcohol blast burner to seal test tube up to 800~1000 DEG C.
During firing, the firepower of alcohol blast burner is only adjusted to maximum, and firing could carry out, remaining firing can fail, Such as hear that as firepower reaches maximum during deafening " he ... ..he ... " sound.
(3) adjustment of state before test tube is fired
Test tube to be fired is taken out, on the other hand by vice, on the other hand by test tube, the positions of vice holding test tubes for nozzle to It at lower 1/3~1/6, holds at the positional distance test tube bottom upward 1/6 of test tube, this position is to be conducive to hand to make power, will be tried Pipe tilts 30 °~50 ° angles, and upward 1/2~1/3 disposition in test tube bottom is fired in flame.
2. the firing of insect specimen test tube
(1) heating and softening of test tube
Test tube is placed in flame and is rotated repeatedly clockwise or counterclockwise for 360 °, makes test tube tube wall homogeneous heating, during heating Between continue 1~3min.
Examination is twisted, if can turn easily, it was demonstrated that test tube softens completely, you can is entered in next step, is otherwise continued to soften.
When examination is twisted, test tube mouth is fixed with vice, remains stationary as test tube, the other end grasps test tube bottom, control Dynamics processed should not be too large, if can turn easily, illustrates that test tube softens completely, can carry out next step operation.
It is uniform that test tube surrounding need to continue rotary heating, is screwed again after its completely softening;Screwing need to coordinate simultaneously with stretching It carries out, and dynamics should not be too large, dynamics is excessive, and test tube can then crack.
When circumgyration stretch test tube caliber is less than former caliber 1/2, rotation can suitably increase with stretching dynamics, make weld point fast Speed is closed, and eventually forms spiral shape sealing that is wide under one and becoming narrow gradually.
The spiral number of turns formed are rotated generally in 5~10 circles;And overstocked, entire spiral shape sealing height is not easy per spiral spacer 10~15mm, long to easily snap off damage, too short to be not easy to fire, and sealing part is often thicker, when cooling, is easy to crack.
In addition, in sintering procedure, liquid level is needed far from flame, and liquid level end is away from 10~15mm of flame on general test tube slant.
(2) rotation and sintering of test tube;
Certain stretching is carried out while rotation, hand-held test tube bottom end is rotated, and rotation is toward the clockwise direction or the inverse time Needle direction, rotation are carried out at the same time with stretching, and vice end is fixed nozzle and stretched, and is slowly stretched, final to draw Distance is stretched for 0.5~1.5cm, and the length according to sealing determines, convenient for the rotation and sealing of test tube.Long sealing part wall is relatively thin, It is not suitable for, shortwall is thicker excessively is also not suitable for, until test tube is closed completely.
Test tube twists 3~5 circles again after being closed, it is ensured that and it completely encloses, is stopped rotating by calcination point slowly far from flame with the external world, Stretching test tube simultaneously makes calcination point top be completely disengaged with lower part, after disengaging, calcination point a bit of very thin shape glass formed above Column, fiberglass column diameter are 0.2~0.5cm, and length is 1~1.5cm, and it is meticulous that diameter is not easy, meticulous easily bad, excessive It is not easy to operate and fire successfully.
Glass column is put into flame to soften, is bent after softening with vice, and is suitably squeezed.
The heating of step (1) test tube and the rotation of softening to step (2) test tube make calcination point top with sintering, stretching test tube In all stage being detached from lower part, flame cannot be left at the calcination of test tube, it is ensured that test tube is heated evenly everywhere, so that simple Easy test tube is successful.
(3) cooling and inspection of test tube
The test tube baked is put on rack for test tube and is cooled down, places 10~15min, takes out and is inverted after cooling, make calcination point Downward, up-down vibration repeatedly, checks whether leakage, if firing success without if, otherwise fires failure.
3. the maintenance and preservation of insect test tube sample
The test tube sample baked is put into the interior Riker mount for containing cotton, is positioned over room temperature, dry environment.
Embodiment 2
The difference lies in try during twisting and screwing, heat-insulated safety gloves in hand strap embodiment 2 with embodiment 1;And Spiral shape sealing shape can be other shapes, can be nearly taper, elongated cylindrical.
Embodiment 3
Embodiment 3 and embodiment 1 the difference lies in, vice is replaced with heat-resisting clamper, including nipper plier, Iron fixture;Heating apparatus (alcohol blast burner) is changed to other heating units that can reach equal emollescence, is sprayed including oxygen Rifle, butane spray gun, liquefaction gas spray gun.
Finally it should be noted that:The above embodiments are merely illustrative of the technical solutions of the present invention, rather than its limitations;Although The present invention is described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that:It still may be used To modify to the technical solution recorded in foregoing embodiments or carry out equivalent replacement to which part technical characteristic; And these modification or replace, various embodiments of the present invention technical solution that it does not separate the essence of the corresponding technical solution spirit and Range.

Claims (9)

1. a kind of easy insect test tube sample production method, which is characterized in that it in turn includes the following steps:
The early-stage preparations of S1, insect test tube sample
The early-stage preparations of insect test tube include:
(1), sample in kind prepares and handles;
Wherein, the sample in kind preparation includes with processing
Step a, according to the test tube of the form of insect, length selection different model specification;
Step b, the insect specimen made is put into test tube bottom;
Step c, preservation liquid is poured into, liquid level is made to be located at the 1/3 of test tube and is stopped;
Step d, residual liquid more than test tube liquid level is made to be heated repeatedly on alcolhol burner, until residual liquid evaporation is complete;
Step e, the test tube in step d is put into rack for test tube, cooling for use;
(2), alcohol blast burner prepares and adjusts;
Step a, it takes out alcohol blast burner, be packed into absolute ethyl alcohol;
Step b, it lights a fire to alcohol blast burner in step a;
Step c, after flame stabilization, the flame of alcohol blast burner is adjusted, its firepower is made to reach maximum;
(3), the adjustment of state before test tube is fired;
Step a, it will wait that firing (1) sample in kind prepares and handle, the test tube in step e takes out;
Step b, the test tube in step a is clamped with vice;
Step c, vice is held, by test tube at bottom 1/2~1/3, is placed in flame and is fired;
The firing of S2, insect specimen test tube
(1) heating and softening of test tube;
Step a, test tube is placed in calcination in flame, and rotated repeatedly;
Step b, the test tube in step a is made to continue 1~3min of calcination on flame, makes tube wall homogeneous heating;
Step c, examination is twisted, until test tube can turn easily;
(2) rotation and sintering of test tube;
Step a, certain stretching is carried out while rotation, hand-held test tube bottom end is rotated, and vice end is fixed nozzle and carried out It stretches, slow stretching, until test tube is closed completely;
Step b, 3~5 circles are twisted again, stops rotating, calcination point is slowly moved away from flame, while stretch test tube, make weld point to examination Nozzle is completely disengaged with weld point to test tube bottom, until calcination point a bit of fiberglass column formed above;
Step c, the glass column in step b is put into flame and carries out calcination, bent after softening with vice and suitably squeezed Pressure;
(3) cooling and inspection of test tube;
Step a, by the rotation and sintering of (2) test tube, the test tube that is baked in step c is put into rack for test tube and is cooled down, after cooling It takes out test tube to be inverted, make at sintering downward;
Step b, the test tube in up-down vibration step a repeatedly, checks whether leakage;
The maintenance and preservation of S3, insect test tube sample
Step a, by the firing of S2 insect test tube sample test tubes, the cooling and inspection of (3) test tube, in step b, the test tube that bakes Sample is put into the Riker mount for containing cotton;
Step b, by the Riker mount in step a be placed in room temperature, drying environment in preserve.
A kind of 2. easy insect test tube sample production method according to claim 1, which is characterized in that the S1 (1) sample in kind prepares and processing, the test tube specification in step a is 18mm × 180mm, 15mm × 150mm, 12mm × 120mm, Tetra- kinds of 10mm × 100mm.
A kind of 3. easy insect test tube sample production method according to claim 1, which is characterized in that the S1 (3) adjustment of state before test tube is fired, vice clip position is at test tube mouth 1/3~1/6 in step b, holds test tube Up at 1/6, test tube tilts 30 °~50 ° angles for bottom.
A kind of 4. easy insect test tube sample production method according to claim 1, which is characterized in that the S2 wine Smart blowtorch prepares and adjustment, in the heating and softening of (1) test tube in the rotation and sintering of step a, step b, step c, (2) test tube Step a, step b is operated on alcohol blast burner flame.
A kind of 5. easy insect test tube sample production method according to claim 1, which is characterized in that the S2 wine Smart blowtorch prepares and adjustment, the heating and softening of (1) test tube, and test tube surrounding need to continue rotary heating in step c, treat that it is completely soft It is screwed again after change.
A kind of 6. easy insect test tube sample production method according to claim 1, which is characterized in that the S2 wine Smart blowtorch prepares and adjustment, rotates in the rotation and sintering of (2) test tube, in step a and is carried out at the same time with stretching, dynamics should not mistake Greatly, when circumgyration stretch makes test tube caliber be less than former caliber 1/2, rotation can suitably increase with stretching dynamics, make calcination point rapid It is closed, forms the nearly taper sealing of spiral that is wide and becoming narrow gradually under one.
A kind of 7. easy insect test tube sample production method according to claim 6, which is characterized in that the spiral Number is 5~10 circles, and unsuitable overstocked per the interval between spiral coil, is divided into 1.5~3mm.
A kind of 8. easy insect test tube sample production method according to claim 6, which is characterized in that the nearly cone Shape seals high 10~15mm.
A kind of 9. easy insect test tube sample production method according to claim 1, which is characterized in that the S2 elder brothers In the firing of worm test tube sample test tube, test tube is placed in calcination on flame in step a, and liquid level is needed far from flame, test tube slant upper liquid Face 10~15mm of positional distance flame location.
CN201810124196.3A 2018-02-07 2018-02-07 A kind of easy insect specimen production method Pending CN108182859A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201810124196.3A CN108182859A (en) 2018-02-07 2018-02-07 A kind of easy insect specimen production method

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Application Number Priority Date Filing Date Title
CN201810124196.3A CN108182859A (en) 2018-02-07 2018-02-07 A kind of easy insect specimen production method

Publications (1)

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CN108182859A true CN108182859A (en) 2018-06-19

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Publication number Priority date Publication date Assignee Title
US20030030677A1 (en) * 2001-07-10 2003-02-13 Norbert Hirneisen Method and device for identifying species of animals and/or plants, and/or types of minerals, as well as of parts of them or products made from them, as well as the location of their occurrence
CN101218909A (en) * 2008-01-28 2008-07-16 河南中医学院 Original plant humid preparation producing method
US7465535B2 (en) * 2004-06-11 2008-12-16 National Institute Of Agrobiological Sciences Dehydration procedures for induction of cryptobiosis in insect larvae
CN101831183A (en) * 2010-05-20 2010-09-15 蒙昭芳 Sealant of dipping specimen and technology thereof
CN203652254U (en) * 2013-11-19 2014-06-18 遵义市林业科学研究所 Insect specimen box

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030030677A1 (en) * 2001-07-10 2003-02-13 Norbert Hirneisen Method and device for identifying species of animals and/or plants, and/or types of minerals, as well as of parts of them or products made from them, as well as the location of their occurrence
US7465535B2 (en) * 2004-06-11 2008-12-16 National Institute Of Agrobiological Sciences Dehydration procedures for induction of cryptobiosis in insect larvae
CN101218909A (en) * 2008-01-28 2008-07-16 河南中医学院 Original plant humid preparation producing method
CN101831183A (en) * 2010-05-20 2010-09-15 蒙昭芳 Sealant of dipping specimen and technology thereof
CN203652254U (en) * 2013-11-19 2014-06-18 遵义市林业科学研究所 Insect specimen box

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Title
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