CN108181474B - Continuous detection analyzer - Google Patents

Continuous detection analyzer Download PDF

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Publication number
CN108181474B
CN108181474B CN201711446714.5A CN201711446714A CN108181474B CN 108181474 B CN108181474 B CN 108181474B CN 201711446714 A CN201711446714 A CN 201711446714A CN 108181474 B CN108181474 B CN 108181474B
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content detector
transfer arm
suction
head
suction nozzle
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CN108181474A (en
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储建华
高霞
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Shanghai Heshang Scientific Instrument Co., Ltd
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Shanghai Heshang Scientific Instrument Co Ltd
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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/92Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving lipids, e.g. cholesterol, lipoproteins, or their receptors

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
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  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)

Abstract

The invention discloses a continuous detection analyzer, comprising: the loading platform is provided with a protein content detector and a lipopolysaccharide content detector; the device comprises a first transverse shaft conveying arm, a second transverse shaft conveying arm and a flushing device, wherein the first transverse shaft conveying arm is synchronously connected with the first longitudinal shaft conveying arm and the second longitudinal shaft conveying arm; the second transverse conveying arm is provided with a reagent kit, and a plurality of liquid samples to be detected are arranged in the reagent kit; the device comprises a liquid sample to be detected, a protein content detector, a lipopolysaccharide content detector, a washing device and a flushing head, wherein a suction nozzle is arranged at the lower end of the pipettor and moves among the liquid sample to be detected, the protein content detector and the lipopolysaccharide content detector, washing liquid is arranged in the flushing device, and the flushing head moves between the protein content detector and the lipopolysaccharide content detector. The invention effectively solves the problems of single detection function and low detection efficiency of the protein analyzer.

Description

Continuous detection analyzer
Technical Field
The invention relates to an analysis and detection device, in particular to a continuous detection analyzer.
Background
The protein analyzer is used for rapidly determining the content of protein in a sample, is one of common devices for medical detection tests, and has the characteristics of rapidness, simplicity, convenience, sensitivity, accuracy, standardization, trace and the like. However, at present, a general protein analyzer on the market has a complex structure, is expensive, has low measurement efficiency, can only measure samples to be measured one by one, cannot realize continuous automatic detection, increases the workload of workers, and is easy to increase the influence of thought factors on measurement results. On the other hand, the conventional protein analyzer has a single function, only has a function of measuring protein content, and does not measure the content of lipopolysaccharide in a sample to be measured, which further increases the detection cost.
Disclosure of Invention
Aiming at the technical problems, the invention discloses a continuous detection analyzer, which effectively solves the problems of single detection function and low detection efficiency of a protein analyzer.
To achieve these objects and other advantages in accordance with the purpose of the invention, there is provided a continuous detection analyzer comprising:
the loading platform is erected on the analyzer base, and a protein content detector and a lipopolysaccharide content detector are arranged on the loading platform;
the first transverse transfer arm is horizontally erected on the loading platform, a first longitudinal transfer arm and a second longitudinal transfer arm are synchronously connected to the first transverse transfer arm, a liquid transfer device is arranged at the lower end of the first longitudinal transfer arm, and a flusher is arranged at the lower end of the second longitudinal transfer arm;
the second transverse conveying arm is arranged on the object carrying platform at the lower end of the first transverse conveying arm, a reagent box is placed on the second transverse conveying arm, and a plurality of liquid samples to be detected are arranged in the reagent box;
the device comprises a liquid sample to be detected, a protein content detector, a lipopolysaccharide content detector, a washing device and a flushing head, wherein a suction nozzle is arranged at the lower end of the pipettor and moves among the liquid sample to be detected, the protein content detector and the lipopolysaccharide content detector, washing liquid is arranged in the flushing device, and the flushing head moves between the protein content detector and the lipopolysaccharide content detector.
Preferably, the first transverse transfer arm is arranged on the loading platform through a support frame, and the first longitudinal transfer arm and the second longitudinal transfer arm are vertically connected to the moving end of the first transverse transfer arm.
Preferably, the pipette is arranged at the moving end of the first longitudinal transfer arm, the flusher is arranged at the moving end of the second longitudinal axis conveyor, and the suction nozzle and the flushing head move up and down in the same plane.
Preferably, the loading platform is further provided with a sample sucking head disc, and the sample sucking head disc, the protein content detector, the lipopolysaccharide content detector, the suction nozzle and the flushing head are in the same plane.
Preferably, the sample suction head disc is provided with sample suction head loading ports and sample suction head unloading ports at intervals, a plurality of sample suction heads are arranged in the sample suction head loading ports, the suction nozzles are in butt joint with the sample suction heads one by one in the sample suction head loading ports, and the sample suction heads loaded by the suction nozzles are unloaded in the sample suction head unloading ports.
Preferably, the second transverse conveying arm is perpendicular to the first transverse conveying arm, the second transverse conveying arm penetrates through the support, a carrying disc is arranged on the second transverse conveying arm in a sliding mode, a positioning groove is formed in the carrying disc, the reagent box is arranged in the positioning groove, a fixing arm is arranged on the carrying disc outside the positioning groove in a rotating mode, and the fixing arm is clamped on the side wall of the reagent box in a clamping mode.
Preferably, a first reflective optical coupler and a second reflective optical coupler are arranged on the support, the first reflective optical coupler is aligned with the reagent kit, and the second reflective optical coupler is aligned with the lower end space of the suction nozzle.
Preferably, the lower end of the liquid transfer device is also provided with a heater for heating the suction nozzle.
The invention at least comprises the following beneficial effects:
1. the provided continuous detection analyzer adopts the three-dimensional moving mechanism, realizes automatic continuous detection on a sample to be detected, improves the detection efficiency of the analyzer, reduces the workload of operators, reduces the interference of human factors and improves the detection precision;
2. the space occupied by the sample adding device is reduced, so that the fluorescent device system is more miniaturized, the moving range of sample adding is wider, the movement in the sample adding process is quicker, the moving precision is high, and the working efficiency of the analyzer is obviously improved;
3. the white content detector and the lipopolysaccharide content detector are arranged at the same time, so that the dual detection functions are integrated, the application range of the analyzer is wider, and the detection cost is low;
4. the flusher is arranged, the white content detector and the lipopolysaccharide content detector are flushed before each detection, the next detection precision is not influenced, and therefore continuous detection of samples is achieved.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Drawings
FIG. 1 is a schematic view of the structure of an analyzer according to the present invention.
Detailed Description
The present invention is further described in detail below with reference to the attached drawings so that those skilled in the art can implement the invention by referring to the description text.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Fig. 1 shows an implementation form of the present invention, which includes:
the loading platform 10 is erected on the analyzer base, and a protein content detector 18 and a lipopolysaccharide content detector 19 are arranged on the loading platform side by side;
a first transverse transfer arm 11 horizontally erected on the loading platform 10 through a support 20, a first longitudinal transfer arm 13 and a second longitudinal transfer arm 14 connected to a moving end of the first transverse transfer arm respectively, the first longitudinal transfer arm and the second longitudinal transfer arm being perpendicular to the loading platform, a liquid transfer device 131 arranged on the moving end of the first longitudinal transfer arm, a flushing device 141 arranged on a moving end of the second longitudinal transfer arm, and the first longitudinal transfer arm and the second longitudinal transfer arm operating independently;
the second transverse transfer arm 12 is arranged on the object platform at the lower end of the first transverse transfer arm, a reagent box 122 is placed on the moving end of the second transverse transfer arm, a plurality of liquid samples to be detected are arranged in the reagent box, and the reagent box 123 is movably arranged at the lower end of the pipettor;
the lower end of the liquid transfer device is provided with a suction nozzle 132, the liquid transfer device is used for continuously sucking the sample to be detected through the suction nozzle, the suction nozzle moves among the sample to be detected, the protein content detector and the lipopolysaccharide content detector, a washing liquid is arranged in the flusher, the lower end of the flusher is provided with a flushing head 142, the washing liquid is sprayed out from the flushing head, and the flushing head moves between the protein content detector and the lipopolysaccharide content detector.
The specific detection process is as follows:
the method comprises the steps that a kit containing a plurality of liquid samples to be detected is arranged at the moving end of a second transverse conveying arm, a first transverse conveying arm 11, a second transverse conveying arm 12 and a first longitudinal conveying arm 13 are controlled at the same time, a suction nozzle moves among the liquid samples to be detected, a protein content detector and a lipopolysaccharide content detector, so that the liquid samples are taken by a liquid transfer machine, after the liquid samples are taken, the same liquid to be detected is partially moved into the protein content detector, and partially moved into the lipopolysaccharide content detector to measure the protein content and the lipopolysaccharide content, so that the liquid transfer machine can automatically move in the X, Y, Z axis direction to finish the sample adding process, and the protein content and the lipopolysaccharide content can be measured simultaneously, the double detection functions are integrated, the application range of an analyzer is wider, and the detection cost is low; meanwhile, after the protein content detection and the lipopolysaccharide content detection of a single liquid sample to be detected are finished, the first transverse transmission arm 11 and the second longitudinal transmission arm 14 are controlled, the washing head is respectively aligned to the protein content detector and the lipopolysaccharide content detector, the protein content detector and the lipopolysaccharide content detector are washed by using the dish washing liquid, so that the next detection precision is not influenced, and after the washing is finished, the pipettor performs the sampling and detecting processes of the next liquid sample to be detected, so that the continuous detection of the samples is realized, and the detection efficiency is improved.
In another embodiment, the suction nozzle 132 and the rinsing head 142 move up and down in the same plane, and the sample sucking head disk 17 is further disposed on the stage, and the sample sucking head disk, the protein content detector, the lipopolysaccharide content detector, the suction nozzle and the rinsing head are in the same plane, so that the suction nozzle can perform an accurate pipetting process, and the rinsing head can perform accurate cleaning on the protein content detector and the lipopolysaccharide content detector, thereby improving the control accuracy of the analyzer and further improving the detection efficiency.
In another embodiment, the sample suction head tray 17 is provided with a sample suction head loading port 171 and a sample suction head unloading port 172 at intervals, the sample suction head loading port is internally provided with a plurality of sample suction heads, the sample suction heads are continuously ejected from the sample suction head loading port 171, when the pipettor is about to perform pipetting, the suction nozzle is aligned with the sample suction head loading port 171, the suction nozzle is controlled to descend, the sample suction head is sleeved on the suction nozzle, then the pipetting process is performed, after the pipetting is completed, the suction nozzle is aligned with the sample suction head unloading port 172, the sample suction head unloading port 172 is provided with a tripping mechanism, when the suction nozzle sleeved with the sample suction head extends into the sample suction head unloading port 172, the tripping mechanism locks the sample suction head, the sample suction head and the suction nozzle are separated along with the ascending of the suction nozzle, so that the used sample suction head is discarded in the sample suction head unloading port 172, and when the suction nozzle performs the next sampling of the liquid to be measured, a new sample suction head is loaded in the sample suction head loading port 171, the suction nozzles are in butt joint with the sample suction heads one by one in the sample suction head loading port 171, the sample suction heads loaded by the suction nozzles are unloaded in the sample suction head unloading ports, after each liquid to be detected is detected, the flusher respectively cleans the protein content detector and the lipopolysaccharide content detector, and after the cleaning is completed, the next liquid to be detected is detected, so that continuous sampling and detection processes are realized, the detection processes are complemented and interfered each time, and the detection precision is improved.
In another embodiment, the second transverse conveying arm is perpendicular to the first transverse conveying arm, the second transverse conveying arm penetrates through the support to ensure that the suction nozzle can sample the whole reagent kit, a carrying disc 121 is slidably arranged on the second transverse conveying arm, a positioning groove 123 is formed in the carrying disc, the reagent kit 122 is arranged in the positioning groove, a fixing arm 124 is rotatably arranged on the carrying disc outside the positioning groove, and after the reagent kit is arranged in the positioning groove, the fixing arm is tightly clamped on the side wall of the reagent kit, so that the reagent kit is fixed with the carrying disc, and the situation that the suction nozzle cannot enter the reagent kit to align with a liquid sample to be detected due to shaking of the reagent kit is avoided.
In another embodiment, the support 20 is provided with a first reflective optical coupler 15 and a second reflective optical coupler 16, the first reflective optical coupler is aligned with the reagent kit and is used for detecting whether a liquid sample to be detected is installed in the reagent kit, determining the position of the liquid sample to be detected on the reagent kit, after the determination, controlling the suction nozzles to sample and detect one by one so as to improve sampling and detecting speed, and the second reflective optical coupler is aligned with the space at the lower end of the suction nozzle to detect whether the suction nozzle carries a sample suction head or not so as to ensure the reliability of the sampling and detecting processes and avoid mutual interference between the detection results caused by the repeated use of the sample suction head, sampling when the sample suction head is not installed, and the like.
In another embodiment, the lower end of the pipettor is further provided with a heater for heating the suction nozzle, and the heater is used for heating the liquid to be detected of the sample suction head so as to keep the liquid to be detected at an ideal temperature, improve the activity of the liquid to be detected and improve the detection precision.
From the above, the continuous detection analyzer provided by the invention adopts the three-dimensional moving mechanism, realizes the automatic continuous detection of the sample to be detected, improves the detection efficiency of the analyzer, reduces the workload of operators, reduces the interference of human factors, and improves the detection precision; meanwhile, the space occupied by the sample adding device is reduced, so that the fluorescent device system is more miniaturized, the moving range of sample adding is wider, the movement in the sample adding process is quicker, the moving precision is high, and the working efficiency of the analyzer is obviously improved; furthermore, a white content detector and a lipopolysaccharide content detector are arranged, so that the dual detection functions are integrated, the application range of the analyzer is wider, and the detection cost is low; and finally, a flusher is arranged, and the white content detector and the lipopolysaccharide content detector are flushed before each detection, so that the next detection precision is not influenced, and the continuous detection of the sample is realized.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the invention pertains, and further modifications may readily be made by those skilled in the art, it being understood that the invention is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.

Claims (6)

1. A continuous detection analyzer, comprising:
the loading platform is erected on the analyzer base, and a protein content detector and a lipopolysaccharide content detector are arranged on the loading platform;
the first transverse transfer arm is horizontally erected on the loading platform, a first longitudinal transfer arm and a second longitudinal transfer arm are synchronously connected to the first transverse transfer arm, a liquid transfer device is arranged at the lower end of the first longitudinal transfer arm, and a flusher is arranged at the lower end of the second longitudinal transfer arm;
the second transverse conveying arm is arranged on the object carrying platform at the lower end of the first transverse conveying arm, a reagent box is placed on the second transverse conveying arm, and a plurality of liquid samples to be detected are arranged in the reagent box;
the lower end of the liquid transferring device is provided with a suction nozzle, the suction nozzle moves among the liquid sample to be detected, the protein content detector and the lipopolysaccharide content detector, a washing liquid is arranged in the flusher, the lower end of the flusher is provided with a flushing head, and the flushing head moves between the protein content detector and the lipopolysaccharide content detector;
still be provided with the suction appearance head dish on the objective platform, suction appearance head dish, albumen content detector, lipopolysaccharide content detector, suction nozzle and flushing head are in the coplanar, the interval is provided with suction appearance head load port and suction appearance head uninstallation mouth on the suction appearance head dish, be provided with a plurality of suction appearance heads in the suction appearance head load port, the suction nozzle is in dock with the suction appearance head one by one in the suction appearance head load port, the suction appearance head that the suction nozzle loaded is in unload in the suction appearance head uninstallation mouth.
2. The continuous inspection analyzer of claim 1, wherein said first lateral transfer arm is mounted to said carrier platform by a support bracket, and said first and second longitudinal transfer arms are vertically connected to a moving end of said first lateral transfer arm.
3. The continuous detection analyzer of claim 2, wherein the pipette is disposed at a moving end of a first longitudinal transfer arm, the flusher is disposed at a moving end of a second longitudinal transfer arm, and the suction nozzle and the flushing head move up and down in the same plane.
4. The continuous detection analyzer of claim 3, wherein the second lateral transfer arm is perpendicular to the first lateral transfer arm and penetrates through the support, a tray is slidably disposed on the second lateral transfer arm, a positioning slot is disposed on the tray, the reagent cartridge is disposed in the positioning slot, and a fixing arm is rotatably disposed on the tray outside the positioning slot and clamped on the side wall of the reagent cartridge.
5. The continuous detection analyzer of claim 4, wherein a first reflective optical coupler and a second reflective optical coupler are disposed on the support, the first reflective optical coupler is aligned with the reagent cartridge, and the second reflective optical coupler is aligned with the lower end space of the suction nozzle.
6. The continuous detection analyzer of claim 5, wherein the pipette lower end is further provided with a heater for heating the suction nozzle.
CN201711446714.5A 2017-12-27 2017-12-27 Continuous detection analyzer Active CN108181474B (en)

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CN108181474B true CN108181474B (en) 2020-07-03

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106053864A (en) * 2015-04-07 2016-10-26 豪夫迈·罗氏有限公司 Reagent management system
CN106574935A (en) * 2014-08-06 2017-04-19 烟台澳斯邦生物工程有限公司 Reagent carrier unit, adapter and method for handling reagent carrier unit
CN206396189U (en) * 2017-01-13 2017-08-11 珠海市银科医学工程股份有限公司 Small size automatic liquid relief platform
CN206531862U (en) * 2017-01-09 2017-09-29 深圳市美侨医疗科技有限公司 Protein analyzer

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016675A1 (en) * 1990-04-06 1991-10-31 Applied Biosystems, Inc. Automated molecular biology laboratory
EP0479448A3 (en) * 1990-10-02 1992-12-23 Beckman Instruments, Inc. Magnetic separation device
JP2014508034A (en) * 2011-01-28 2014-04-03 インテグラ バイオサイエンシズ コープ. Multi-channel well plate filling system

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106574935A (en) * 2014-08-06 2017-04-19 烟台澳斯邦生物工程有限公司 Reagent carrier unit, adapter and method for handling reagent carrier unit
CN106053864A (en) * 2015-04-07 2016-10-26 豪夫迈·罗氏有限公司 Reagent management system
CN206531862U (en) * 2017-01-09 2017-09-29 深圳市美侨医疗科技有限公司 Protein analyzer
CN206396189U (en) * 2017-01-13 2017-08-11 珠海市银科医学工程股份有限公司 Small size automatic liquid relief platform

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