JP2841914B2 - Biochemical automatic analyzer - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to an automatic analyzer for performing a biochemical test on a sample such as blood or urine, and more particularly to a single-multi type automatic analyzer.

[0002]

2. Description of the Related Art In a biochemical automatic analyzer, a sample such as blood is dispensed into a reaction container, and a reagent is added to the sample for each measurement item, and a reaction between the sample and the reagent is detected. In a reagent dispensing mechanism for dispensing a reagent into a reaction container, a plurality of reagents are dispensed by a common reagent dispenser in order to simplify an apparatus configuration. At this time, in order to reduce the influence of contamination between reagents, the relationship between items that are susceptible to contamination among reagents is set in the apparatus in advance, and when analyzing reagents that are highly affected by contamination between reagents, A method has been proposed in which the analysis order is controlled so that a plurality of items are analyzed in such an order that the dispenser is thoroughly cleaned and the influence of contamination is reduced to a negligible level (Japanese Patent Publication No. 1-15422,
Japanese Patent Publication No. 47746/1994). Some recent automatic analyzers can avoid contamination between reagents, automatically detect contamination over time in a reaction vessel, and automatically perform washing with a detergent.

[0003]

The present inventor examined the relationship between a large number of measurement items and detergents. As a result, the specific measurement items were affected by the specific detergent, and the measured values increased or decreased. And found that some of them were contaminated by detergents. However, in the conventional automatic biochemical analyzer, contamination between reagents is taken into consideration, but contamination by detergent is not taken into consideration. An object of the present invention is to provide an automatic biochemical analyzer that can avoid not only the effects of contamination between reagents but also the effects of detergent contamination.

In the present invention, the relationship between the type of detergent and the analysis items that are easily affected by contamination by each detergent is set in the control unit in advance, and the control unit determines whether or not there is an interference between the next analysis item and the detergent. Is checked, and a type of detergent that does not affect the next analysis item is selected, or an analysis item that does not interfere with the selected detergent is controlled for the next analysis. As for measurement items that may be affected by contamination between reagents, the effect of contamination between reagents can be reduced by washing the reagent dispenser and reaction vessel well and changing the order of the measurement items as before. I do. In addition, when performing cleaning with a detergent, a check is performed based on data relating to the contamination relationship between the type of detergent and the measurement item set in the control unit in advance, and when it is determined that the detergent is contaminated, In order to avoid the influence, for example, processing such as changing the order of analysis or using another detergent is performed.

[0005]

FIG. 1 shows an example of an automatic biochemical analyzer to which the present invention is applied. A sample such as blood is placed in a sample container, and a sample rack 2 in which a plurality of sample containers are arranged.
Is moved along the conveyor path 4 of the belt conveyor type. The transport path 4 includes a forward path 4a for transferring the sample rack 2 from left to right in the figure and a return path 4b for transferring the sample rack from right to left. In the figure, a sample rack supply section 6 for sending the sample rack 2 to the forward path 4a is provided at the left end of the forward path 4a, and a storage section 8 for storing the sample rack 2 after the measurement is completed is provided at the left end of the return path 4b. Have been. In the figure, a rack standby section 10 is provided at the right end of the transport path 4 for temporarily storing the sample rack 2 sent on the outward path 4a and sending it to the return path 4b. The sample rack 2 is stopped at the sample suctioning <br/> position of analysis units 12 a, 12 b during transfer of the forward path 4a, dispensed into the reaction tube of analysis units 12 a, 12 b. In the return path 4b, a sample that needs to be retested is redispensed according to the measurement result of the sample dispensed and measured in the outward path 4a.

The two analysis units 12 along the transport path 4
a and 12b are arranged. Both have the same structure. In each analysis unit, a reaction disk 14 in which a reaction vessel 15 also serving as a cuvette is disposed is disposed near the transport path 4, and the reaction disks 1 are disposed in the transport paths 4a and 4b.
A stop device (not shown) for stopping the sample rack 2 at the sample suction position near the sample suction position 4 is provided. Conveyance path 4a or 4
In order to dispense a sample from the sample rack 2 stopped on b into the reaction container 15, a pipetter 16 having a nozzle is arranged. Each analysis unit 12a, 12b is provided with two turntable type reagent storages 18a, 18b for dispensing reagents into the reaction container 15, and the reagent storages 18a, 18b store reagents in the reaction container 15. Dispensers 20a and 20b for dispensing are provided. A washing mechanism 22 is provided for washing the reaction vessel after the analysis with the reaction disk 14. The reaction disk 14 is provided with an optical analyzer for measuring the reaction of the reaction container 15 containing the sample and the reagent, but is not shown.

The sample rack supply unit 6 and the storage unit 8 are provided with an interface and a CPU 24. Each of the analysis units 12a and 12b has an interface and a CPU.
PUs 26 a and 26 b are provided, and the standby unit 10 is also provided with an interface and a CPU 28. The CPUs 24, 26a, 26b and 28 are main CPUs.
30. The main CPU 30 further has C
The RT 32, the keyboard 34, and the printer 36 are connected.

[0008] Data relating to the items affected by contamination between reagents and data relating to the relationship between the detergent and the items contaminated by the detergent have been input to the main CPU 30 in advance, and these data are stored in the CPU 26 of each analysis unit.
a and 26b, respectively, and the order of analysis in each analysis unit 12a, 12b and the type of detergent used in the reaction vessel 15 and the dispensers 20a, 20b are determined and determined.

[0009] Contamination between reagents is well known, and means for avoiding contamination between reagents have already been taken, so here are examples of items affected by detergents. For example, the item alkaline phosphatase is affected by detergent Contaminone LA or Contaminone L (these are alkaline detergents and a product of Wako Pure Chemical Industries, Ltd.). The value appears to be smaller, such as 110 due to the influence, and similarly, the measurement value was correctly 200 to 180 due to the detergent. Further, for example, the item PL
(Phospholipids) were affected by Detergent Green 99L-100T (a product of Clean Chemical Co., Ltd.), for example, the measurement value was correctly 182, but now 215, and the measurement value was correctly 252. Things 2
In this case, the numerical value appears larger, such as 75.

Next, the operation of this embodiment will be described.
Reagents and detergents necessary for the measurement of each item are set in the reagent storages 18a and 18b of the analysis unit. C of each analysis unit
In the PUs 26a and 26b, data relating to contamination between reagents and data relating to contamination by a detergent are downloaded from the main CPU 30 for each item.

When the sample racks 2 are arranged in the supply unit 6 and the operation is started from the keyboard 34, the sample rack 2 is washed by the washing mechanism 22 with washing water. Water is put into the washed reaction vessel 15 and a blank measurement is performed. When the reaction container 15 to which the blank measurement has been performed and the water has been sucked moves to the sample dispensing position, the sample rack 2 is sent along the forward path 4a of the transport path and stopped at the sample dispensing position. For the measurement item to be measured, the sample is dispensed into the reaction container 15 by the pipetter 16 by the sample amount determined for each measurement item. After dispensing the sample, the nozzle of the pipetter 16 moves to a washing pot not shown in the figure, and the inside and outside of the nozzle are washed with pure water. Thereafter, the dispensing of the next item or following another sample order Tsugido one analyte takes place to another reaction vessel 15.

In the automatic analyzer according to the present invention,
In the sample aspiration by the pipettor being performed continuously, in the dispensing operation, to determine the next measurement item to be aspirated, when checking whether it interferes with the immediately preceding dispensation,
If the immediately preceding dispensing is a detergent dispensing, the data on the contamination by the detergent also considers whether or not the measurement item is sufficient. Here, if you say "just before dispensing"
This means contamination with the reagent probe, but the same judgment applies to the reaction container to be dispensed, and also considers the contamination with the measurement item or detergent used immediately before (one lap before) in the reaction container. Can be. Contamination data is set for each probe and reaction vessel.

When the reaction container 15 in which the sample has been dispensed from the reaction disk 14 moves to the reagent dispensing position, a predetermined amount of the reagent is aspirated by the dispenser 20a or 20b and dispensed into the reaction container 15. . After dispensing, the probes of the dispensers 20a and 20b are moved to a washing position not shown in the drawing, and the inside and outside of the probes are washed with pure water. Thereafter, the dispensers 20a and 20b shift to the next reagent dispensing operation.

The sample rack 2 travels on the outward path and waits in the standby section 10, waits for the analysis result of the sample, is sent out to the return path 4 b, and is stored in the storage section 8. While moving on the return path 4b, the sample that needs to be retested is stopped at the sample dispensing position, and the sample is dispensed again. In the reaction disk 14, the measurement unit measures the absorbance of the reaction solution in which the sample and the reagent are mixed. At the washing position, the reaction solution which has been analyzed is suctioned and discharged at the washing position, washed with water, drained from the reaction container, and prepared as a new sample reaction container.

The present invention is not limited to a biochemical automatic analyzer of a type in which a sample rack moves by a belt conveyor as shown in FIG. 1, but various other types of biochemical automatic analyzers conventionally used. Can be applied to

[0016]

According to the present invention, not only contamination between reagents can be avoided, but also contamination with a detergent can be avoided, so that the automatic washing function can be fully utilized. Although having a cleaning capacity superior is as a detergent, for the particular detergents such as causing pollution for items, with respect to items that do not affect it becomes possible to effectively utilize the detergent.

[Brief description of the drawings]

FIG. 1 is a schematic perspective view showing an example of an automatic biochemical analyzer to which the present invention is applied.

[Explanation of symbols]

2 sample rack 4 rack transport path 6 rack supply unit 8 storage unit 10 standby unit 12a, 12b analysis unit 14 reaction disk 15 reaction container 16 pipettor 18a, 18b reagent storage 20a, 20b dispenser 22 cleaning mechanism 24, 26a, 26b, 28 interface And C
PU 30 Main CPU

Claims (1)

(57) [Claims] 1. A sample dispenser for dispensing a sample into a reaction container, a reagent dispenser for dispensing a reagent with a reagent dispenser common to a plurality of types of reagents, a reaction container and a reagent dispenser after the reaction. A cleaning mechanism that can be used for cleaning by selectively using a reagent dispenser after injection from a plurality of types of detergents, and a control unit in which a relationship between various detergents and analysis items that are easily affected by contamination by the detergents is provided. The control unit checks whether there is interference between the next analysis item and the detergent, and selects a type of detergent that does not affect the next analysis item, or selects an analysis item that does not interfere with the selected detergent for the next analysis. Biochemical automatic analyzer characterized by such control.