CN108179141A - A kind of plant rennet extracting method and plant rennet obtained - Google Patents
A kind of plant rennet extracting method and plant rennet obtained Download PDFInfo
- Publication number
- CN108179141A CN108179141A CN201810265151.8A CN201810265151A CN108179141A CN 108179141 A CN108179141 A CN 108179141A CN 201810265151 A CN201810265151 A CN 201810265151A CN 108179141 A CN108179141 A CN 108179141A
- Authority
- CN
- China
- Prior art keywords
- plant rennet
- plant
- solid
- rennet
- extracting
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/63—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from plants
Abstract
The present invention relates to a kind of plant rennet extracting method and plant rennet obtained, the method includes：The slurries of plant or crushed material are pressed into solid-liquid ratio 1 with water:Water extraction is carried out after 5~20 mixing, collects extracting solution；Gained extracting solution passes sequentially through plate and frame filter press and ceramic membrane filter, and the filter cloth pore size of plate and frame filter press is 100~150 mesh, and the pore size of ceramic membrane is 50~200nm；First time hyperfiltration treatment is carried out to filtrate using the ultrafiltration membrane that molecular cut off is 30~100KDa, collects filtrate, second of hyperfiltration treatment is carried out to filtrate using the ultrafiltration membrane that molecular cut off is 5~15KDa, collects trapped fluid；The concentrated acquisition concentrate of trapped fluid, concentrate are freeze-dried up to plant rennet.The method of the invention have many advantages, such as it is simple for process, environmentally safe, can scale industrial production, the enzyme activity of protease and purity it is good.The advantages of plant rennet is active good, purity is high, no solvent residue reaches the requirement of food, daily use chemicals and medical industry.
Description
Technical field
The present invention relates to protease to extract field, in particular to a kind of plant rennet extracting method and obtained
Plant rennet.
Background technology
Plant rennet has protein efficiently single-minded resolution characteristic, available for the hydrolysis of protein, tenderization,
Beer clarification, marine products processing prepare detergent and cosmetics, and certain plants protease such as papain has pharmacology in itself
Effect, available for treatment inflammation and indigestion.Therefore, plant rennet exists extensive in food, daily use chemicals and field of medicaments
Application.The improvement of plant rennet extraction process, especially industrial extraction method, is of great significance.
At present, the method for extraction and purification of plant rennet is more, and the plant rennet that can carry out industrialized production carries
It takes there are mainly three types of methods：Kaolin absorption method, the tannin precipitation method and flocculence.
Wherein, the technical process of kaolin absorption method is as shown in Figure of description Fig. 1.As shown in Figure 1, kaolin is inhaled
The production technology of attached method and operation are all more complicated, and raw materials consumption is more, and required equipment is also more, kaolin with saltout technique to ring
Border pollutes, and enzyme activity overall recovery is low, but product quality is preferable, and purity is higher, and the enzyme activity of product such as papain exists
4.0×106More than U/g can reach pharmaceutical grade.Due to adding kaolin and use is saltoutd technique, ring is polluted the environment
It is larger to protect investment.
The technical process of the tannin precipitation method is as shown in Figure of description Fig. 2.Tannin precipitation method technique and operation are than high
Ridge local method is simple, and raw materials consumption is few, and required equipment is few, but the enzyme activity rate of recovery is relatively low, the enzyme activity of product such as papain
3.5×106~4.0 × 106Between U/g, portioned product can reach food-grade.Simultaneously as tannin is added in production, to ring
Border pollutes, and environmental protection investments are larger.
Flocculence is to add certain compound in papaya latex glue extracting solution, which can generate with papain
The compound of slightly solubility, so as to make enzyme Precipitation from solution.
He Jiqin etc. is had studied with flocculation technique Purification of Papain.The experimental results showed that under appropriate conditions into
Row flocculation treatment can make the yield that papain is walked at this up to 95~97%.But this method extraction specificity is not strong, is made
Purity of papain it is not high, impurity content is higher.
It can be seen that existing plant rennet extracting method there are complex process, cause serious pollution to the environment, enzyme activity and purity
The defects of not high.This field is urgently improved existing plant rennet extracting method, to obtain it is a kind of it is simple for process,
Pollution-free, enzyme activity and purity height, plant rennet extracting method capable of being industrialized.
In view of this, it is special to propose the present invention.
Invention content
The first object of the present invention is to provide a kind of plant rennet extracting method, the method have it is simple for process,
It is environmentally safe, can scale industrial production, the advantages that enzyme activity of protease and purity are good.
The second object of the present invention is to provide a kind of plant rennet as made from aforementioned extracting method, the plant egg
The advantages of enzyme is active good, purity is high, no solvent residue in vain reaches the requirement of food, daily use chemicals and medical industry.
In order to realize the above-mentioned purpose of the present invention, spy uses following technical scheme：
A kind of plant rennet extracting method, the described method comprises the following steps：
(1) slurries of plant or crushed material are pressed into solid-liquid ratio 1 with water:Water extraction is carried out after 5~20 mixing, collects extraction
Liquid；
(2) gained extracting solution passes sequentially through plate and frame filter press and ceramic membrane filter, the filter cloth aperture of the plate and frame filter press
Size is 100~150 mesh, and the pore size of the ceramic membrane is 50~200nm；
(3) first time hyperfiltration treatment is carried out to the filtrate using the ultrafiltration membrane that molecular cut off is 30~100KDa, received
Collect filtrate, second of hyperfiltration treatment is carried out to the filtrate using the ultrafiltration membrane that molecular cut off is 5~15KDa, collects retention
Liquid；
(4) the concentrated acquisition concentrate of the trapped fluid, the concentrate are freeze-dried up to plant rennet.
Different from conventional plant rennet extracting method as shown in Figure of description Fig. 3, the method for the invention is whole
Using film process, including plate and frame filter press is used to carry out separation of solid and liquid, clarified using ceramic membrane and bacteria removing, with
And carry out hyperfiltration treatment using ultrafiltration membrane.Film process mode is mild to the processing mode of albumen, will not during isolating and purifying
Plant rennet is caused to inactivate, the loss of protease is small, and the enzyme activity of extract is high.Meanwhile film process mode was isolating and purifying
Organic solvent or other impurities will not be introduced in journey, therefore, plant rennet organic solvent-free residual obtained, quality is high, can
It is directly used in food, daily use chemicals and field of medicaments.In addition, the method for the invention is without using kaolin, tannin, it will not be to environment
It pollutes, belongs to environment-friendly type extracting method.Furthermore the method for the invention is successively using the super of two kinds of different molecular weight cut off
Filter membrane carries out hyperfiltration treatment, can all remove the macromolecular foreign protein in extracting solution and small molecular weight impurity, purification effect is good.
Preferably, in plant rennet extracting method as described above, when carrying out water extraction, packet is added in mixed liquor
Include sodium benzoate and the enzymatic protective reagent of EDTA-2Na, the quality of the sodium benzoate and the EDTA-2Na in the mixed liquor
Score is 0.1%~0.2%.
Preferably, in plant rennet extracting method as described above, the sodium benzoate and the EDTA-2Na exist
Mass fraction in the mixed liquor is 0.15%.
Preferably, in plant rennet extracting method as described above, in step (3), first time hyperfiltration treatment uses
Be molecular cut off be 40~60KDa ultrafiltration membrane, second of hyperfiltration treatment use molecular cut off be 7~12KDa
Ultrafiltration membrane.
Preferably, in plant rennet extracting method as described above, in step (3), first time hyperfiltration treatment uses
Be molecular cut off be 50KDa ultrafiltration membrane, second of hyperfiltration treatment use molecular cut off be 10KDa ultrafiltration
Film.
Preferably, in plant rennet extracting method as described above, the plant rennet be selected from papain,
Actinidin, ficin, momordica grosvenori proteolytic or bromelain.
Preferably, in plant rennet extracting method as described above, the plant rennet is papain, is walked
Suddenly the material mixed in (1) with water is pawpaw slurries, and the solid-liquid ratio of the pawpaw slurries and water is 1:7~12 preferably 1:10, water
Extracting mode is preferably stirred to react 1.5h to be stirred to react 1~2h.
Preferably, in plant rennet extracting method as described above, the plant rennet is momordica grosvenori proteolytic,
In the step (1), the solid-liquid ratio of arhat juice and water is 1:5~10, preferably 1:7, water extracting mode for be stirred to react 1~
2h is preferably stirred to react 1.5h.
Preferably, in plant rennet extracting method as described above, the plant rennet is ficin,
In the step (1), fig crushed material presses feed liquid with pH=4~5, the NaCl aqueous solutions that mass fraction is 0.5%~1.2%
Than 1:10~20 mixing；
Preferably, fig crushed material presses solid-liquid ratio 1 with pH=4.5, the NaCl aqueous solutions that mass fraction is 0.9%:15
Mixing.
Preferably, in plant rennet extracting method as described above, the plant rennet is Kiwi berry plant egg
White enzyme, the step (1) specifically include：By kiwi fruit crushed material and pH=4~5, the NaCl that mass fraction is 22~25%
Aqueous solution presses solid-liquid ratio 1:4~7 mixing, are stirred to react 1~2h, and separation of solid and liquid after 4~8h of low-temperature precipitation, gained solid matter is used
The buffer solution of pH=6~8 is redissolved to get extracting solution；
Preferably, in plant rennet extracting method as described above, the step (1) specifically includes：By Kiwi berry
Fruit powder mince with pH=4~5, mass fraction be 24% NaCl aqueous solutions by solid-liquid ratio 1:5 mixing, are stirred to react 1.5h, low
Separation of solid and liquid after temperature precipitation 6h, the gained solid matter buffer solution of pH=7 are redissolved to get extracting solution.
Preferably, in plant rennet extracting method as described above, the plant rennet is pineapple plant albumen
Enzyme, in step (1), pineapple crushed material presses solid-liquid ratio 1 with water:7~12 preferably 1:10 mixing, water extracting mode is is stirred to react 1
~2h, is preferably stirred to react 1.5h.
The method of the invention also to papain, momordica grosvenori proteolytic, ficin, Actinidin and
The specific extracting method of bromelain is advanced optimized, wherein, in extraction ficin and Actinidin
The NaCl aqueous solutions of Shi Caiyong acidity are handled, and can significantly improve the extraction effect of protease, wherein, extract fig egg
During white enzyme, the NaCl of low concentration primarily serves the effect for keeping enzyme activity in aqueous solution, and when extracting Actinidin, aqueous solution
The NaCl of middle and high concentration primarily serves the effect for promoting Actinidin low-temperature precipitation after water carries.In conclusion this
The invention above method optimizes the specific extraction process of plant rennet, and the extracting method after optimization has recovery rate and enzyme
The characteristics of higher living.
The invention further relates to the plant rennets made of preceding method.Plant rennet of the present invention has the plant
The advantages of object protease is active good, purity is high, no solvent residue reaches the requirement of food, daily use chemicals and medical industry.
Compared with prior art, beneficial effects of the present invention are：
(1) plant rennet extracting method of the present invention have it is simple for process, environmentally safe, can large-scale industrial
Production, the advantages that enzyme activity of protease and purity are good.
(2) the method for the invention optimizes specific extraction process, and the extracting method after optimization has recovery rate
The characteristics of with enzyme activity higher.
(3) the advantages of plant rennet of the present invention is active good, purity is high, no solvent residue, reaches food, day
Change the requirement with medical industry
Description of the drawings
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution of the prior art
Embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, in being described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, can also be obtained according to these attached drawings other attached drawings.
Fig. 1 is the process flow chart that kaolin absorption method produces plant rennet；
Fig. 2 is the process flow chart that the tannin precipitation method produce plant rennet；
Fig. 3 is the process flow chart that the method for the invention produces plant rennet.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person, the condition suggested according to normal condition or manufacturer carry out.Reagents or instruments used without specified manufacturer is
The conventional products obtained can be bought by city.
Embodiment 1
A kind of extracting method of papain, the method comprise the steps of：
Papaya puree and process water are pressed into solid-liquid ratio 1:10 mixing add in enzymatic protective reagent sodium benzoate and EDTA-2Na, described
The mass fraction of sodium benzoate and the EDTA-2Na in the mixed liquor is 0.1%~0.2%, is stirred to react 1.5h,
Up to extracting solution；
Gained extracting solution passes sequentially through plate and frame filter press and ceramic membrane filter, the filter cloth pore size of the plate and frame filter press
For 150 mesh, the pore size of the ceramic membrane is 50nm；
First time hyperfiltration treatment is carried out to the filtrate using the ultrafiltration membrane that molecular cut off is 50KDa, filtrate is collected, makes
Second of hyperfiltration treatment is carried out to the filtrate with the ultrafiltration membrane that molecular cut off is 10KDa, collects trapped fluid；
The concentrated acquisition concentrate of trapped fluid, the concentrate are freeze-dried up to papain.
Embodiment 2
A kind of extracting method of momordica grosvenori proteolytic, the method comprise the steps of：
New fresh fructus momordicae is cleaned, and is crushed, and centrifugation obtains arhat juice after removing slag, and arhat juice and process water are pressed solid-liquid ratio
1:10 mixing, are stirred to react 1.5h to get extracting solution；
Gained extracting solution passes sequentially through plate and frame filter press and ceramic membrane filter, the filter cloth pore size of the plate and frame filter press
For 150 mesh, the pore size of the ceramic membrane is 200nm；
First time hyperfiltration treatment is carried out to the filtrate using the ultrafiltration membrane that molecular cut off is 50KDa, filtrate is collected, makes
Second of hyperfiltration treatment is carried out to the filtrate with the ultrafiltration membrane that molecular cut off is 10KDa, collects trapped fluid；
The concentrated acquisition concentrate of trapped fluid, the concentrate are freeze-dried up to momordica grosvenori proteolytic.
Embodiment 3
A kind of extracting method of Actinidin, the method comprise the steps of：
Kiwi fruit through crushing and pH=4.5, the NaCl aqueous solutions that mass fraction is 24% are pressed into solid-liquid ratio 1:5 is mixed
It closes, is stirred to react 1.5h, separation of solid and liquid after low-temperature precipitation 6h, the gained solid matter buffer solution of pH=7 is redissolved to get extraction
Liquid；
Gained extracting solution passes sequentially through plate and frame filter press and ceramic membrane filter, the filter cloth pore size of the plate and frame filter press
For 100 mesh, the pore size of the ceramic membrane is 100nm；
First time hyperfiltration treatment is carried out to the filtrate using the ultrafiltration membrane that molecular cut off is 50KDa, filtrate is collected, makes
Second of hyperfiltration treatment is carried out to the filtrate with the ultrafiltration membrane that molecular cut off is 10KDa, collects trapped fluid；
The concentrated acquisition concentrate of trapped fluid, the concentrate are freeze-dried up to Actinidin.
Embodiment 4
A kind of extracting method of ficin, the method comprise the steps of：
By the fig Chinese olive of crushing with pressing solid-liquid ratio 1 with pH=4.5, the NaCl aqueous solutions that mass fraction is 0.9%:15
Mixing, is stirred to react 1.5h to get extracting solution；
Gained extracting solution passes sequentially through plate and frame filter press and ceramic membrane filter, the filter cloth pore size of the plate and frame filter press
For 100 mesh, the pore size of the ceramic membrane is 100nm；
First time hyperfiltration treatment is carried out to the filtrate using the ultrafiltration membrane that molecular cut off is 50KDa, filtrate is collected, makes
Second of hyperfiltration treatment is carried out to the filtrate with the ultrafiltration membrane that molecular cut off is 10KDa, collects trapped fluid；
The concentrated acquisition concentrate of trapped fluid, the concentrate are freeze-dried up to ficin.
Embodiment 5
A kind of extracting method of bromelain, the method comprise the steps of：
Pineapple rhizome after crushing and process water water are pressed into solid-liquid ratio 1:10 mixing, add in enzymatic protective reagent sodium benzoate and
EDTA-2Na, the mass fraction of the sodium benzoate and the EDTA-2Na in the mixed liquor is 0.1%~0.2%,
1.5h is stirred to react to get extracting solution；
Gained extracting solution passes sequentially through plate and frame filter press and ceramic membrane filter, the filter cloth pore size of the plate and frame filter press
For 100 mesh, the pore size of the ceramic membrane is 200nm；
First time hyperfiltration treatment is carried out to the filtrate using the ultrafiltration membrane that molecular cut off is 50KDa, filtrate is collected, makes
Second of hyperfiltration treatment is carried out to the filtrate with the ultrafiltration membrane that molecular cut off is 10KDa, collects trapped fluid；
The concentrated acquisition concentrate of trapped fluid, the concentrate are freeze-dried up to bromelain.
Embodiment 6
With reference to 3 the method for embodiment extraction Actinidin, differ only in, extracting solution obtains in the following manner：
Kiwi fruit through crushing and process water are pressed into solid-liquid ratio 1:5 mixing, are stirred to react 1.5h to get extracting solution.
Embodiment 7
With reference to 4 the method for embodiment extraction ficin, differ only in, extracting solution obtains in the following manner：
Fig Chinese olive through crushing and process water are pressed into solid-liquid ratio 1:15 mixing, are stirred to react 1.5h to get extracting solution.
Experimental example 1
Detect the enzyme activity of protease and the enzyme activity rate of recovery described in Examples 1 to 7, specific detection method and testing result
It is as follows：
Enzyme activity assay method：Using casein as substrate, measure the activity of papain, detection method referring to " Su S N,
Nie H L,Zhu L M et al.Optimization of adsorption conditions of papain on dye
affinity membrane using response surface methodology[J].Bioresource
technology,2009,100(8):2336-2340”。
Enzyme activity determination of recovery rates method：In unit mass raw material plant rennet enzyme activity content and from unit mass original
The plant rennet enzyme activity content extracted in material, the latter and the former ratio are the enzyme activity rate of recovery.
The enzyme activity of 1 plant rennet of table and the enzyme activity rate of recovery
Finally it should be noted that：The above embodiments are only used to illustrate the technical solution of the present invention., rather than its limitations；To the greatest extent
Pipe is described in detail the present invention with reference to foregoing embodiments, but it will be understood by those of ordinary skill in the art that：Its
It can still modify to the technical solution recorded in foregoing embodiments either to which part or all technical features
Carry out equivalent replacement；And these modifications or replacement, various embodiments of the present invention skill that it does not separate the essence of the corresponding technical solution
The range of art scheme.
Claims (10)
1. a kind of plant rennet extracting method, which is characterized in that the described method comprises the following steps：
(1) slurries of plant or crushed material are pressed into solid-liquid ratio 1 with water:Water extraction is carried out after 5~20 mixing, collects extracting solution；
(2) gained extracting solution passes sequentially through plate and frame filter press and ceramic membrane filter, the filter cloth pore size of the plate and frame filter press
For 100~150 mesh, the pore size of the ceramic membrane is 50~200nm；
(3) first time hyperfiltration treatment is carried out to filtrate using the ultrafiltration membrane that molecular cut off is 30~100KDa, collects filtrate, make
Second of hyperfiltration treatment is carried out to the filtrate with the ultrafiltration membrane that molecular cut off is 5~15KDa, collects trapped fluid；
(4) the concentrated acquisition concentrate of the trapped fluid, the concentrate are freeze-dried up to plant rennet.
2. plant rennet extracting method according to claim 1, which is characterized in that when carrying out water extraction, mixed liquor
Middle addition includes sodium benzoate and the enzymatic protective reagent of EDTA-2Na, and the sodium benzoate and the EDTA-2Na are in the mixed liquor
In mass fraction be 0.1%~0.2%.
3. plant rennet extracting method according to claim 1, which is characterized in that in step (3), at first time ultrafiltration
Reason uses the ultrafiltration membrane that molecular cut off is the preferred 50KDa of 40~60KDa, and second of hyperfiltration treatment uses retention point
Ultrafiltration membrane of the son amount for the preferred 10KDa of 7~12KDa.
4. plant rennet extracting method according to claim 1, which is characterized in that the plant rennet is selected from pawpaw
Protease, Actinidin, ficin, momordica grosvenori proteolytic or bromelain.
5. plant rennet extracting method as claimed in claim 2, which is characterized in that the plant rennet is Papain
Enzyme, the material mixed with water in step (1) are pawpaw slurries, and the solid-liquid ratio of the pawpaw slurries and water is 1:7~12 preferably 1:
10, water extracting mode is preferably stirred to react 1.5h to be stirred to react 1~2h.
6. plant rennet extracting method according to claim 1, which is characterized in that the plant rennet is Siraitia grosvenorii
Protease, in the step (1), the solid-liquid ratio of arhat juice and water is 1:5~10, preferably 1:7, water extracting mode is stirring
1~2h is reacted, is preferably stirred to react 1.5h.
7. plant rennet extracting method according to claim 1, which is characterized in that the plant rennet is fig
Protease, in the step (1), fig crushed material and pH=4~5, the NaCl that mass fraction is 0.5%~1.2% are water-soluble
Liquid presses solid-liquid ratio 1:10~20 mixing；
Preferably, fig crushed material presses solid-liquid ratio 1 with pH=4.5, the NaCl aqueous solutions that mass fraction is 0.9%:15 mixing.
8. plant rennet extracting method according to claim 1, which is characterized in that the plant rennet is Kiwi berry
Plant rennet, the step (1) specifically include：It is 22~25% by kiwi fruit crushed material and pH=4~5, mass fraction
NaCl aqueous solutions press solid-liquid ratio 1:4~7 mixing, are stirred to react 1~2h, separation of solid and liquid after 4~8h of low-temperature precipitation, gained solid phase
The buffer solution of substance pH=6~8 is redissolved to get extracting solution；
Preferably, the step (1) specifically includes：By kiwi fruit crushed material and pH=4.5, the NaCl that mass fraction is 24%
Aqueous solution presses solid-liquid ratio 1:5 mixing, are stirred to react 1.5h, and separation of solid and liquid after low-temperature precipitation 6h, gained solid matter is with pH=7's
Buffer solution is redissolved to get extracting solution.
9. plant rennet extracting method according to claim 2, which is characterized in that the plant rennet is planted for pineapple
Object protease, in step (1), pineapple crushed material presses solid-liquid ratio 1 with water:7~12 preferably 1:10 mixing, water extracting mode are stirring
1~2h is reacted, is preferably stirred to react 1.5h.
10. the plant rennet made of any one of claim 1~9 the method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810265151.8A CN108179141A (en) | 2018-03-28 | 2018-03-28 | A kind of plant rennet extracting method and plant rennet obtained |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810265151.8A CN108179141A (en) | 2018-03-28 | 2018-03-28 | A kind of plant rennet extracting method and plant rennet obtained |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108179141A true CN108179141A (en) | 2018-06-19 |
Family
ID=62553870
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810265151.8A Pending CN108179141A (en) | 2018-03-28 | 2018-03-28 | A kind of plant rennet extracting method and plant rennet obtained |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108179141A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110089664A (en) * | 2019-05-31 | 2019-08-06 | 广东省广窑记餐饮管理服务有限公司 | The production method of kiln chicken |
| CN110438110A (en) * | 2019-08-14 | 2019-11-12 | 重庆骄王天然产物股份有限公司 | Bromelain, its production method and its processing system |
| CN110747189A (en) * | 2019-10-12 | 2020-02-04 | 南京立顶生物科技有限公司 | Method for rapidly extracting matrix metalloproteinase |
| CN113215137A (en) * | 2021-06-11 | 2021-08-06 | 江苏格局生物医药科技有限公司 | Preparation method of enzyme for pharmaceutical industry |
| CN114052152A (en) * | 2021-09-28 | 2022-02-18 | 海南黎草纪新生物科技有限公司 | Extraction method of plant micromolecular water |
| CN114350644A (en) * | 2021-11-26 | 2022-04-15 | 清大国杰生物科技有限公司 | Plant protein polypeptidase and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3272743B2 (en) * | 1991-04-26 | 2002-04-08 | 三省製薬株式会社 | Placenta or liver extract having stable SOD activity and external preparation composition containing the same |
| CN103602649A (en) * | 2013-11-07 | 2014-02-26 | 陆祥 | Purification method for papain |
| CN104293750A (en) * | 2014-10-15 | 2015-01-21 | 重庆骄王天然产物股份有限公司 | Method for efficiently extracting sterile papain |
| CN106916801A (en) * | 2017-01-20 | 2017-07-04 | 东莞市华琪生物科技有限公司 | A kind of industrialization preparation method of pawpaw enzyme preparation and its application |
-
2018
- 2018-03-28 CN CN201810265151.8A patent/CN108179141A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3272743B2 (en) * | 1991-04-26 | 2002-04-08 | 三省製薬株式会社 | Placenta or liver extract having stable SOD activity and external preparation composition containing the same |
| CN103602649A (en) * | 2013-11-07 | 2014-02-26 | 陆祥 | Purification method for papain |
| CN104293750A (en) * | 2014-10-15 | 2015-01-21 | 重庆骄王天然产物股份有限公司 | Method for efficiently extracting sterile papain |
| CN106916801A (en) * | 2017-01-20 | 2017-07-04 | 东莞市华琪生物科技有限公司 | A kind of industrialization preparation method of pawpaw enzyme preparation and its application |
Non-Patent Citations (3)
| Title |
|---|
| 方瑾: "《生物学科知识与教学能力》", 31 August 2015, 光明日报出版社 * |
| 曹尚银: "《无花果高效栽培与加工利用》", 30 April 2002, 中国农业出版社 * |
| 许英一 等: "《食品化学与分析》", 31 July 2014, 哈尔滨工程大学出版社 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110089664A (en) * | 2019-05-31 | 2019-08-06 | 广东省广窑记餐饮管理服务有限公司 | The production method of kiln chicken |
| CN110438110A (en) * | 2019-08-14 | 2019-11-12 | 重庆骄王天然产物股份有限公司 | Bromelain, its production method and its processing system |
| CN110438110B (en) * | 2019-08-14 | 2021-04-23 | 重庆骄王天然产物股份有限公司 | Bromelain, production method thereof and treatment system thereof |
| CN110747189A (en) * | 2019-10-12 | 2020-02-04 | 南京立顶生物科技有限公司 | Method for rapidly extracting matrix metalloproteinase |
| CN113215137A (en) * | 2021-06-11 | 2021-08-06 | 江苏格局生物医药科技有限公司 | Preparation method of enzyme for pharmaceutical industry |
| CN114052152A (en) * | 2021-09-28 | 2022-02-18 | 海南黎草纪新生物科技有限公司 | Extraction method of plant micromolecular water |
| CN114052152B (en) * | 2021-09-28 | 2023-05-09 | 海南黎草纪新生物科技有限公司 | Extraction method of plant small molecular water |
| CN114350644A (en) * | 2021-11-26 | 2022-04-15 | 清大国杰生物科技有限公司 | Plant protein polypeptidase and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108179141A (en) | A kind of plant rennet extracting method and plant rennet obtained | |
| CN102150737B (en) | Method for preparing high-purity rice protein and acquired products by using same | |
| CN107858393A (en) | A kind of method that polypeptide is extracted from walnut dregs | |
| CN102907558A (en) | Processing method of sea cucumber polypeptide | |
| CN106244658B (en) | Preparation method of sweet potato protein polypeptide | |
| CN103073652B (en) | A kind of extracting method of spirulina polysaccharide | |
| CN109824796A (en) | The extracting and developing purification process of beta-glucosan from oat bran | |
| CN106754834A (en) | A kind of preparation technology of high activity papain | |
| CN103750258A (en) | Method for preparing flavored yeast extracts and polysaccharides from waste beer yeasts | |
| CN110627898B (en) | Extraction process of potato trypsin inhibitor | |
| CN101434981B (en) | Method for preparing vegetable seed peptide with single bioactivity by microbial solid state fermentation | |
| CN109385414B (en) | Purification method of bromelain | |
| CN103789286B (en) | A kind of preparation method of amylase | |
| CN110777173B (en) | Method for preparing momordica grosvenori amino acid by using momordica grosvenori centrifugal waste residues | |
| CN103602649B (en) | A kind of method of purification of papoid | |
| CN106148467B (en) | A kind of preparation method of mussel active peptide | |
| CN107011423A (en) | Protein product and preparation method thereof | |
| CN109021095A (en) | A kind of high-purity is without fishy smell algae blue pigment and the preparation method and application thereof | |
| CN103289968B (en) | From the water-soluble extracting liquid of sweet potato, be separated and prepare the method for polyphenoloxidase, beta-amylase and storage protein | |
| CN109112121B (en) | Method for preparing soybean β -amylase by using soybean protein bean green water | |
| CN113913409A (en) | Compound protease for extracting ginseng extract, preparation method and application process thereof | |
| CN108440635B (en) | Separation method for improving yield of tea saponin | |
| CN107011422A (en) | Protein product and preparation method thereof | |
| CN105831390A (en) | Anthocyanin-rich black rice protein, anthocyanin-rich black rice syrup, and production method of protein and syrup | |
| CN1037698C (en) | Refined enzyme purification process for papain |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180619 |