CN108175797B - A topical Chinese medicinal preparation for treating ocular surface diseases, and its preparation method - Google Patents
A topical Chinese medicinal preparation for treating ocular surface diseases, and its preparation method Download PDFInfo
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Abstract
The invention belongs to the technical field of eye external medicines, and particularly relates to an external traditional Chinese medicine preparation for treating eye surface diseases and a preparation method thereof. The external traditional Chinese medicine preparation for treating the ocular surface diseases is prepared from the following raw materials in parts by weight: 8-10 parts of angelica sinensis, 15-20 parts of long-noded pit viper, 10-15 parts of semen cassiae, 20-30 parts of radix isatidis and 15-20 parts of houttuynia cordata. The invention develops a traditional Chinese medicine formula capable of treating bacterial eye diseases and viral eye diseases simultaneously by using the compatibility principle of traditional Chinese medicines and only using 5 medicinal materials in a matching way, and the effective rate of the medicine reaches more than 90 percent. The invention also provides a preparation method for treating the ocular surface diseases, which adopts sodium bicarbonate solution to soak the medicinal materials, modifies the medicinal materials and accelerates the dissolution of active substances in the medicinal materials.
Description
Technical Field
The invention belongs to the technical field of eye external medicines, and particularly relates to an external traditional Chinese medicine preparation for treating eye surface diseases and a preparation method thereof.
Background
The ocular surface infectious diseases are common diseases and frequently encountered diseases in an eye clinic, wherein bacterial and viral conjunctivitis and keratitis are the most common diseases, such as staphylococcus aureus conjunctivitis, gonococcal conjunctivitis, adenovirus keratoconjunctivitis, herpes simplex virus keratitis and the like. Bacterial keratitis is keratitis caused by sensitive pathogenic bacteria, and in the prior art, once bacterial conjunctivitis keratitis is definitely diagnosed, firstly, antibacterial eye drops sensitive to local frequency points are adopted, secondly, inflammation is inhibited, and operation treatment is needed if necessary. Clinically common antibacterial eye drops comprise quinolones, cephalosporins, aminoglycosides, macrolides and the like, and the medicines have the characteristics of broad spectrum, high sensitivity and strong tissue penetration. However, long-term use of antibiotic drugs such as the quinolones causes drug resistance in bacteria, and antibiotics have poor inhibitory activity against viruses. Viral conjunctivitis keratitis is caused by one or more pathogenic viruses, and the commonly used drugs at present are acyclovir eye drops and ganciclovir eye drops, but the two drugs achieve antiviral effect by inhibiting virus DNA synthesis, but long-term application can cause keratoconjunctival epithelial damage.
Traditional Chinese medicine is a traditional medical treatment mode in China, can condition the whole body of a human body due to small side effect, achieves the effect of treating both principal and secondary aspect of disease, and is still a common medicine up to now. Therefore, if a traditional Chinese medicine preparation capable of treating bacterial and viral conjunctival and corneal inflammation is developed, the use of antibiotics and western medicines can be reduced, and the occurrence of microbial drug resistance can be avoided.
Disclosure of Invention
The invention aims to provide an external traditional Chinese medicine preparation for treating ocular surface diseases and a preparation method thereof, the traditional Chinese medicine preparation can treat bacterial and viral ocular surface diseases simultaneously, the use of antibiotics is reduced, and the occurrence of microbial drug resistance is avoided.
The invention provides an external traditional Chinese medicine preparation for treating ocular surface diseases, which is prepared from the following raw materials in parts by weight: 8-10 parts of angelica sinensis, 15-20 parts of long-noded pit viper, 10-15 parts of semen cassiae, 20-30 parts of radix isatidis and 15-20 parts of houttuynia cordata.
Preferably, the external traditional Chinese medicine preparation for treating ocular surface diseases comprises the following raw materials in parts by weight: 8 parts of angelica, 15 parts of long-noded pit viper, 15 parts of cassia seed, 20 parts of isatis root and 20 parts of cordate houttuynia.
The invention also provides a preparation method of the external traditional Chinese medicine preparation for treating ocular surface diseases, which comprises the following steps:
s1, weighing the following raw materials in parts by weight: 8-10 parts of angelica sinensis, 15-20 parts of long-noded pit viper, 10-15 parts of semen cassiae, 20-30 parts of radix isatidis and 15-20 parts of houttuynia cordata;
s2, mixing the raw materials weighed in the S1, adding 0.6g/100ml of sodium bicarbonate solution which is 10 times of the total weight of the raw materials into the obtained mixture, soaking for 2d, boiling for 10-15 min, filtering out medicine residues, and collecting filtrate;
s3, adding 0.05mol/l hydrochloric acid solution into the filtrate of S2, and adjusting the pH to 7.0 to obtain neutral liquid medicine;
s4, boiling the neutral liquid medicine of S3 for 5-10 min, and performing decolorization treatment to obtain decolorized liquid medicine;
s5, rotationally evaporating the decolored liquid medicine of S4 to 1/10 of the volume of the sodium bicarbonate solution to obtain the external traditional Chinese medicine preparation for treating the ocular surface diseases.
Preferably, in the above method for preparing a topical Chinese medicinal preparation for treating ocular surface diseases, the decolorizing treatment is performed by using a polar adsorbent and a non-polar adsorbent, wherein the polar adsorbent is silica gel, and the non-polar adsorbent is activated carbon or diatomaceous earth.
Preferably, in the preparation method of the external traditional Chinese medicine for treating ocular surface diseases, in S4, after the neutral liquid medicine is boiled for 5-10 min, the neutral liquid medicine sequentially flows through a non-polar adsorbent column and a polar adsorbent column for decolorization, wherein the diameter of the non-polar adsorbent column is 50cm, and the height of the non-polar adsorbent column is 50 cm; the polar adsorbent column had a diameter of 40cm and a height of 60 cm.
Compared with the prior art, the external traditional Chinese medicine preparation for treating the ocular surface diseases and the preparation method thereof provided by the invention have the following beneficial effects:
1. the main functions of angelica sinensis recorded in the Chinese pharmacopoeia are to promote blood circulation and relieve pain; the long-noded pit viper has the main functions of clearing away heat and toxic materials, resisting bacteria and diminishing inflammation; the cassia seed has the main functions of reducing blood pressure, clearing liver and improving vision, the isatis root has the main functions of resisting virus, clearing heat and removing toxicity, and the houttuynia cordata has the main functions of clearing heat and removing toxicity. The invention develops a traditional Chinese medicine formula capable of treating bacterial eye diseases and viral eye diseases simultaneously by utilizing the compatibility principle of traditional Chinese medicines and only using 5 medicinal materials in a matching way, the effective rate of the medicine reaches more than 90 percent, the use of antibiotics is reduced, and the occurrence of microbial drug resistance is avoided.
2. The preparation method comprises the steps of soaking the medicinal materials in a sodium bicarbonate solution, modifying the medicinal materials, accelerating the dissolution of active substances in the medicinal materials, neutralizing the medicinal materials to be neutral by a hydrochloric acid solution, reacting the sodium bicarbonate solution with the hydrochloric acid to generate sodium chloride, water and carbon dioxide, wherein the carbon dioxide overflows through a subsequent boiling step and is reserved in the medicament as sodium ions necessary for an organism and absorbed by the organism when the medicament is taken. In the preparation method, the polar adsorbent and the non-polar adsorbent are used together to adsorb polar pigment and non-polar pigment, and meanwhile, the silica gel, the activated carbon and the diatomite can also be used as filter aids.
Detailed Description
The present invention will be described in detail with reference to specific embodiments, but it should be understood that the scope of the present invention is not limited by the specific embodiments. The test methods not specified in the following examples are generally conducted under conventional conditions, and the materials not specified are commercially available as sources, and the steps thereof will not be described in detail since they do not relate to the invention.
The invention provides an external traditional Chinese medicine preparation for treating ocular surface diseases, which is prepared from the following raw materials in parts by weight: 8-10 parts of angelica sinensis, 15-20 parts of long-noded pit viper, 10-15 parts of semen cassiae, 20-30 parts of radix isatidis and 15-20 parts of houttuynia cordata.
Based on the same inventive concept, the invention also provides a preparation method of the externally applied traditional Chinese medicine for treating ocular surface diseases, which comprises the following steps:
s1, weighing the following raw materials in parts by weight: 8-10 parts of angelica sinensis, 15-20 parts of long-noded pit viper, 10-15 parts of semen cassiae, 20-30 parts of radix isatidis and 15-20 parts of houttuynia cordata;
s2, mixing the raw materials weighed in the S1, adding 0.6g/100ml of sodium bicarbonate solution which is 10 times of the total weight of the raw materials into the obtained mixture, soaking for 2d, boiling for 10-15 min, filtering out medicine residues, and collecting filtrate;
s3, adding 0.05mol/l hydrochloric acid solution into the filtrate of S2, and adjusting the pH to 7.0 to obtain neutral liquid medicine;
s4, boiling the neutral liquid medicine of S3 for 5-10 min, and performing decolorization treatment to obtain decolorized liquid medicine;
s5, rotationally evaporating the decolored liquid medicine of S4 to 1/10 of the volume of the sodium bicarbonate solution to obtain the external traditional Chinese medicine preparation for treating the ocular surface diseases.
The decolorizing treatment is carried out by adopting a polar adsorbent and a non-polar adsorbent, wherein the polar adsorbent is silica gel, and the non-polar adsorbent is activated carbon or diatomite. And (3) after the neutral liquid medicine is boiled for 5-10 min, sequentially flowing through a non-polar adsorbent column and a polar adsorbent column for decolorization.
It should be noted that, in order to prolong the shelf life, 1 ‰ preservative may be added after the preparation of the Chinese medicinal preparation.
Some examples of the externally applied Chinese medicinal preparation for treating ocular surface diseases of the present invention are listed below.
Example 1
An external traditional Chinese medicine preparation for treating eye surface diseases is composed of the following raw materials in parts by weight: 8 parts of angelica, 15 parts of long-noded pit viper, 15 parts of cassia seed, 20 parts of isatis root and 20 parts of cordate houttuynia.
Example 2
An external traditional Chinese medicine preparation for treating eye surface diseases is composed of the following raw materials in parts by weight: 10 parts of angelica, 20 parts of long-noded pit viper, 10 parts of cassia seed, 30 parts of isatis root and 15 parts of cordate houttuynia.
Example 3
An external traditional Chinese medicine preparation for treating eye surface diseases is composed of the following raw materials in parts by weight: 9 parts of angelica, 18 parts of long-noded pit viper, 12 parts of cassia seed, 25 parts of isatis root and 16 parts of cordate houttuynia.
Example 4
An external traditional Chinese medicine preparation for treating eye surface diseases is composed of the following raw materials in parts by weight: 8 parts of angelica, 19 parts of long-noded pit viper, 11 parts of cassia seed, 22 parts of isatis root and 18 parts of cordate houttuynia.
Example 5
An external traditional Chinese medicine preparation for treating eye surface diseases is composed of the following raw materials in parts by weight: 10 parts of angelica, 15 parts of long-noded pit viper, 14 parts of cassia seed, 28 parts of isatis root and 16 parts of houttuynia cordata.
Some examples of the preparation method of the external traditional Chinese medicine for treating ocular surface diseases of the present invention are given below by taking the formulation of example 1 as an example.
Example 6
A preparation method of an external traditional Chinese medicine preparation for treating ocular surface diseases comprises the following steps:
s1, weighing the following raw materials in parts by weight: 8 parts of angelica, 15 parts of long-noded pit viper, 15 parts of cassia seed, 20 parts of isatis root and 20 parts of cordate houttuynia;
s2, mixing the raw materials weighed in the S1, adding 0.6g/100ml of sodium bicarbonate solution which is 10 times of the total weight of the raw materials into the obtained mixture, soaking for 2d, stirring for 3 times during the soaking, boiling for 10min, filtering out dregs, and collecting filtrate;
s3, adding 0.05mol/l hydrochloric acid solution into the filtrate of S2, and adjusting the pH to 7.0 to obtain neutral liquid medicine;
s4, boiling the neutral medicinal liquid of S3 for 5min, and decoloring while the solution is hot to obtain a decolored medicinal liquid;
the decoloring treatment adopts a polar adsorbent and a non-polar adsorbent to decolor, wherein the polar adsorbent is silica gel, and the non-polar adsorbent is activated carbon; boiling neutral medicinal liquid for 5min, sequentially passing through nonpolar adsorbent column and polar adsorbent column for decolorizing, wherein the diameter of the nonpolar adsorbent column is 50cm, the height is 50cm, and the flow rate is 10L/min; the diameter of the polar adsorbent column is 40cm, the height is 60cm, and the flow rate is 10L/min;
s5, rotationally evaporating the decolored liquid medicine of S4 to 1/10 of the volume of the sodium bicarbonate solution to obtain the external traditional Chinese medicine preparation for treating the ocular surface diseases.
Example 7
A preparation method of an external traditional Chinese medicine preparation for treating ocular surface diseases comprises the following steps:
s1, weighing the following raw materials in parts by weight: 8 parts of angelica, 15 parts of long-noded pit viper, 15 parts of cassia seed, 20 parts of isatis root and 20 parts of cordate houttuynia;
s2, mixing the raw materials weighed in the S1, adding 0.6g/100ml of sodium bicarbonate solution which is 10 times of the total weight of the raw materials into the obtained mixture, soaking for 2d, stirring for 4 times during the soaking, boiling for 15min, filtering out dregs, and collecting filtrate;
s3, adding 0.05mol/l hydrochloric acid solution into the filtrate of S2, and adjusting the pH to 7.0 to obtain neutral liquid medicine;
s4, boiling the neutral medicinal liquid of S3 for 10min, and decoloring while the solution is hot to obtain a decolored medicinal liquid;
the decoloring treatment adopts a polar adsorbent and a non-polar adsorbent to decolor, wherein the polar adsorbent is silica gel, and the non-polar adsorbent is activated carbon; boiling neutral medicinal liquid for 10min, sequentially passing through nonpolar adsorbent column and polar adsorbent column for decolorizing, wherein the diameter of the nonpolar adsorbent column is 50cm, the height is 50cm, and the flow rate is 10L/min; the diameter of the polar adsorbent column is 40cm, the height is 60cm, and the flow rate is 10L/min;
s5, rotationally evaporating the decolored liquid medicine of S4 to 1/10 of the volume of the sodium bicarbonate solution to obtain the external traditional Chinese medicine preparation for treating the ocular surface diseases.
Example 8
A preparation method of an external traditional Chinese medicine preparation for treating ocular surface diseases comprises the following steps:
s1, weighing the following raw materials in parts by weight: 8 parts of angelica, 15 parts of long-noded pit viper, 15 parts of cassia seed, 20 parts of isatis root and 20 parts of cordate houttuynia;
s2, mixing the raw materials weighed in the S1, adding 0.6g/100ml of sodium bicarbonate solution which is 10 times of the total weight of the raw materials into the obtained mixture, soaking for 2 days, stirring for 2 times during the soaking period, boiling for 12min, filtering out dregs, and collecting filtrate;
s3, adding 0.05mol/l hydrochloric acid solution into the filtrate of S2, and adjusting the pH to 7.0 to obtain neutral liquid medicine;
s4, boiling the neutral medicinal liquid of S3 for 8min, and decoloring while the solution is hot to obtain a decolored medicinal liquid;
decolorizing with a polar adsorbent and a nonpolar adsorbent, wherein the polar adsorbent is silica gel, and the nonpolar adsorbent is diatomite; boiling the neutral medicinal liquid for 8min, and filtering with plate-and-frame filter filled with diatomite and silica gel as both decolorizing agent and filter aid.
S5, rotationally evaporating the decolored liquid medicine of S4 to 1/10 of the volume of the sodium bicarbonate solution to obtain the external traditional Chinese medicine preparation for treating the ocular surface diseases.
Example 9
A method for preparing a topical Chinese medicinal formulation for treating ocular surface diseases (formulation of example 1), comprising the steps of:
s (1), weighing the following raw materials in parts by weight: 8 parts of angelica, 15 parts of long-noded pit viper, 15 parts of cassia seed, 20 parts of isatis root and 20 parts of cordate houttuynia;
s (2), mixing the raw materials weighed in the step S (1), adding water which is 10 times of the total weight of the raw materials into the obtained mixture, soaking for 2d, stirring for 3 times during soaking, boiling for 10min, filtering out dregs, and collecting filtrate;
s (3), boiling the neutral liquor obtained in the step S (2) for 5min, and decoloring while the liquor is hot to obtain a decolored liquor;
the decoloring treatment adopts a polar adsorbent and a non-polar adsorbent to decolor, wherein the polar adsorbent is silica gel, and the non-polar adsorbent is activated carbon; boiling neutral medicinal liquid for 5min, sequentially passing through nonpolar adsorbent column and polar adsorbent column for decolorizing, wherein the diameter of the nonpolar adsorbent column is 50cm, the height is 50cm, and the flow rate is 10L/min; the diameter of the polar adsorbent column is 40cm, the height is 60cm, and the flow rate is 10L/min;
s5, rotationally evaporating the decolored liquid medicine of S4 to 1/10 of the volume of the water in the S (2) to obtain the external traditional Chinese medicine preparation for treating the ocular surface diseases.
The effects of the external preparation of the present invention will be described below by taking the formulations of examples 1 to 5 as examples.
First, bacteriostasis experiment
Staphylococcus aureus, staphylococcus epidermidis and diphtheroid bacillus are used as experimental strains, and the strains are all from a microorganism preservation center CGMCC. Respectively preparing culture solution of Staphylococcus aureus, Staphylococcus epidermidis and diphtheroid bacillus, and diluting thallus concentration to 106cfu/ml, soaking filter paper disc with diameter of 0.5cm in Chinese medicinal preparation (different Chinese medicinal preparation for each group) for 10 min; preparing beef extract peptone plates, coating culture solutions for respectively preparing staphylococcus aureus, staphylococcus epidermidis and diphtheroid bacillus on the beef extract peptone plates under the condition of no bacteria, then placing the soaked filter paper in the center of the plates, culturing for 14h at 37 ℃, and measuring the size of a bacteriostatic zone.
Wherein, the traditional Chinese medicine preparation of the group of the examples 1 to 5 adopts the formula of the example 1 to 5 and the preparation method of the example 6, the traditional Chinese medicine preparation of the group of the comparison 1 adopts the formula of the example 1 and the preparation method of the example 9, and the traditional Chinese medicine preparation of the group of the comparison 2 adopts the penicillin solution of 35mg/100 ml. The results are shown in Table 1.
TABLE 1 size of zone of inhibition for different Chinese medicinal preparations
The results in table 1 show that the bacteriostatic effect of the traditional Chinese medicine prepared by the formula of examples 1-5 and the preparation method of example 6 on staphylococcus aureus, staphylococcus epidermidis and diphtheroids is equivalent to that of a 35mg/100ml penicillin solution, but the bacteriostatic effect of the control group 1 is poor. The reason is that the preparation method of the embodiment 6 firstly adopts the sodium bicarbonate solution to soak the medicinal materials, modifies the medicinal materials, accelerates the dissolution of active substances in the medicinal materials, and then neutralizes the medicinal materials by the hydrochloric acid solution. The control group 1 had no soaking step with sodium bicarbonate solution, so the bacteriostatic effect was poor.
Second, the influence on the microcirculation of the eye conjunctiva of the rabbit
30 rabbits are selected by a rabbit eye conjunctiva microvascular fluorometry, the male and female clapboards weigh 1.8-2.5kg and are randomly divided into 3 groups, wherein in the gastric perfusion traditional Chinese medicine in the example 1 group, the traditional Chinese medicine is prepared by weighing each medicinal material in the formula of the example 1, grinding the medicinal materials into 100 meshes of medicinal powder, and the gastric perfusion amount is 1.5g/kg, and continuously administering for 7 days; example 2 group of gavage traditional Chinese medicine, the traditional Chinese medicine is prepared by weighing the medicinal materials of the formula of example 2, grinding into 100-mesh powder, the gavage amount is 1.5g/kg, and continuously administering for 7 days; the control group was administered with saline of equal quality by gavage for 7 days. 1h after the last gastric lavage, 10% sodium fluorescein normal saline solution is injected into the ear vein of the rabbit at 1ml/kg (equivalent to that of fluorescein being injected at 100mg/kg), and the time of fluorescence appearing in eye conjunctiva of the rabbit is recorded, so that the result shows that the fluorescence appearing time of the group in example 1 is 7.58s (the average value of 10 rabbits), the fluorescence appearing time of the group in example 2 is 7.70s (the average value of 10 rabbits), the fluorescence appearing time of the control group is 12.79s (the average value of 10 rabbits), and the result shows that the microcirculation blood flow of eye conjunctiva of the rabbit is accelerated remarkably and the blood flow supply is improved remarkably in the groups in example 1 and example 2, and the difference from the control group is remarkable (P is less than 0.05).
Third, clinical cases
Shen Shi, male, 32 years old, clinically diagnosed as Staphylococcus aureus conjunctivitis with symptoms of staphylococcal blepharitis etc., the external traditional Chinese medicine preparation of example 1 was used 4 times a day, 3 drops each time, after 1 week the symptoms disappeared, recovered to health, without side effect.
Plum, female, 29 years old, clinically diagnosed as gonococcal conjunctivitis, the external traditional Chinese medicine preparation of example 1 was used once an hour and 4 drops each time, and symptoms disappeared after 4 days, so that the health was recovered without side effects.
The externally-applied traditional Chinese medicine preparation of the example 1 is used for 4 times a day, 3 drops each time, and the symptoms disappear after 10 days, so that the health is recovered, and no side effect is caused.
The externally applied traditional Chinese medicine preparation in the example 1 is used for 4 times a day and 3 drops each time, meanwhile, the formula in the example 1 (each weight part represents 1 g, for example 8 parts of angelica are weighed) is decocted and filtered according to a conventional traditional Chinese medicine method, the traditional Chinese medicine liquid is taken 3 times a day and 100ml each time, and after 6 days, symptoms disappear, and the health is recovered without side effects.
The clinical cases show that the traditional Chinese medicine prepared by the medicine formula and the preparation method provided by the invention can effectively treat viral or bacterial ocular surface diseases. In addition, 100 cases of herpes simplex keratitis patients are selected, the patients are administrated by referring to the administration mode of the above-mentioned scholar, the curative effect is observed after 1 week, wherein 80 patients are cured, 10 patients are effectively relieved (the eye irritation symptom is basically completely disappeared, the fluorescein staining is punctiform or the dendritic corneal epithelium pathological change part is slightly spotted), and the effective rate of the traditional Chinese medicine preparation is 90%.
It is to be understood that where the claims and specification refer to a range of values, such ranges are to be considered as being within the scope of the present invention, including the endpoints, and that even though preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (5)
1. The application of the combination of angelica, long-noded pit viper and cassia seed in preparing the external traditional Chinese medicine for treating eye surface diseases is characterized in that the external traditional Chinese medicine is prepared from the following raw materials in parts by weight: 8-10 parts of angelica sinensis, 15-20 parts of long-noded pit viper, 10-15 parts of semen cassiae, 20-30 parts of radix isatidis and 15-20 parts of houttuynia cordata; the ocular surface disease is caused by bacteria or viruses.
2. The use of the combination of angelica, agkistrodon, cassia tora according to claim 1 in the preparation of a topical traditional Chinese medicine for the treatment of ocular surface diseases is characterized by consisting of the following raw materials in parts by weight: 8 parts of angelica, 15 parts of long-noded pit viper, 15 parts of cassia seed, 20 parts of isatis root and 20 parts of cordate houttuynia.
3. The use of the combination of angelica, agkistrodon and cassia seed according to claim 1 in the preparation of an external traditional Chinese medicine for treating ocular surface diseases, wherein the preparation method of the external traditional Chinese medicine for treating ocular surface diseases comprises the following steps:
s1, weighing the following raw materials in parts by weight: 8-10 parts of angelica sinensis, 15-20 parts of long-noded pit viper, 10-15 parts of semen cassiae, 20-30 parts of radix isatidis and 15-20 parts of houttuynia cordata;
s2, mixing the raw materials weighed in the S1, adding 0.6g/100ml of sodium bicarbonate solution which is 10 times of the total weight of the raw materials into the obtained mixture, soaking for 2d, boiling for 10-15 min, filtering out medicine residues, and collecting filtrate;
s3, adding 0.05mol/l hydrochloric acid solution into the filtrate of S2, and adjusting the pH to 7.0 to obtain neutral liquid medicine;
s4, boiling the neutral liquid medicine of S3 for 5-10 min, and performing decolorization treatment to obtain decolorized liquid medicine;
s5, rotationally evaporating the decolored liquid medicine of S4 to 1/10 of the volume of the sodium bicarbonate solution to obtain the external traditional Chinese medicine preparation for treating the ocular surface diseases.
4. The use of the combination of angelica, agkistrodon and cassia seed as claimed in claim 3, wherein the decolorizing treatment is carried out by using polar adsorbent and non-polar adsorbent, the polar adsorbent is silica gel, and the non-polar adsorbent is activated carbon or diatomite.
5. The use of the combination of angelica, agkistrodon and cassia seed as claimed in claim 4, in the preparation of a topical Chinese medicinal preparation for treating ocular surface diseases, wherein in S4, after the neutral medicinal liquid is boiled for 5-10 min, the neutral medicinal liquid is passed through a non-polar adsorbent column and a polar adsorbent column in sequence for decolorization, wherein the diameter of the non-polar adsorbent column is 50cm, and the height of the non-polar adsorbent column is 50 cm; the polar adsorbent column had a diameter of 40cm and a height of 60 cm.
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| CN1965877A (en) * | 2006-11-06 | 2007-05-23 | 赵因 | Chinese medicinal eye drop for treating bacteria and virus infected conjunctivitis |
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| CN1965877A (en) * | 2006-11-06 | 2007-05-23 | 赵因 | Chinese medicinal eye drop for treating bacteria and virus infected conjunctivitis |
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| Title |
|---|
| 角膜炎中医文献及证治规律研究;朱晓林;《中国博士学位论文全文数据库 卫生医药科技辑》;20091115;68-69 * |
| 鱼腥草注射液与板蓝根冲剂治疗流行性角结膜炎;戴丽彬等;《中国民间疗法》;20010531;第9卷(第05期);47-48 * |
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