CN108175427A - Rodent anxiety level test experiments track display method and device - Google Patents
Rodent anxiety level test experiments track display method and device Download PDFInfo
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- CN108175427A CN108175427A CN201711433633.1A CN201711433633A CN108175427A CN 108175427 A CN108175427 A CN 108175427A CN 201711433633 A CN201711433633 A CN 201711433633A CN 108175427 A CN108175427 A CN 108175427A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/16—Devices for psychotechnics; Testing reaction times ; Devices for evaluating the psychological state
- A61B5/165—Evaluating the state of mind, e.g. depression, anxiety
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/0002—Inspection of images, e.g. flaw detection
- G06T7/0012—Biomedical image inspection
- G06T7/0014—Biomedical image inspection using an image reference approach
- G06T7/0016—Biomedical image inspection using an image reference approach involving temporal comparison
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/20—Analysis of motion
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- G—PHYSICS
- G06—COMPUTING; CALCULATING OR COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
- G06T2207/10016—Video; Image sequence
Abstract
The invention discloses a kind of rodent anxiety level test experiments track display method and devices.This method includes：Obtain rodent experiment video,Wherein,Experiment video includes rodent the action trail in anxiety level test experiments,Rodent experiment video is analyzed according to preset time interval,Obtain experiment picture and corresponding experiment moment,Obtain the position of rodent pixel residing in picture is tested,And the variable quantity of the position of the pixel according to residing for rodent in the experiment picture at adjacent experiment moment,Determine instantaneous relative velocity of the rodent in pixel,According to the position of rodent pixel residing in picture is tested,Draw movement locus of the rodent in video is tested,And instantaneous relative velocity of the rodent at the position of pixel is shown in movement locus,Improve the analysis efficiency and precision of analysis of rodent anxiety level test experiments video.
Description
Technical field
The present embodiments relate to analysis of experimental data technology more particularly to a kind of test of rodent anxiety level are real
Test track display method and device.
Background technology
Anxiety disorder is one group of disease to feel anxiety and fear is the experience of main mental state, is had to personal and society huge
Negative effect.The clinical effective treatment means lacked for anxiety disorder at present, the complete mechanism of neural level also do not illustrate.It is burnt
The research and development of worry disease Mechanism Study and treatment technology are all strongly dependent on the foundation of effective animal model.It is made by animal stress
After Anxiety Model, spacious field experiment and elevated plus-maze test are the classical experiments for the detection of animal anxiety level, quilt
It is widely used in frightened, anxiety and depressed corelation behaviour research, investigates the phobic anxiety state of animal.Fig. 1 is tested for spacious field
Ideograph.As shown in Figure 1, by rodent, for example, mouse, is put into the chest 11 that bottom surface size is 50*50cm, and advise
Determine in chest bottom surface, region 12 centered on intermediate 20*20cm, other regions are fringe region 13, are observed small in a period of time
Behavior of the mouse in the chest.Fig. 2 is elevated plus-maze test ideograph.As shown in Fig. 2, the experimental model includes distance
Ground 60cm and open arms 21 parallel to the ground and closure arm 22, open arms 21 and closure arm 22 are mutually perpendicular to.Mouse is put
In any region for entering the two arms, behavior of the mouse in open arms 21 and closure arm 22 in a period of time is observed.To upper
The process for stating two kinds of experiments is recorded a video, and obtains experiment video.
At present, can be by the experiment video input Anymaze softwares in two kinds of experiments, Anymaze softwares can be to reality
It tests video to be analyzed, determines the behavior of rodent：It determines in spacious field experiment, rodent enters central area and side
The number in edge region, the time and, average speed of distance track and walking etc.；It determines in elevated plus-maze test, grinding tooth
Class animal enter open arms residence time and explore distance, into closure arm residence time and explore distance and, walking
Average speed etc..Anymaze is after the behavior of rodent is determined, with the output data of table or figure.
But for current anxiety level behaviouristics data analysing method, Anymaze can only show grinding tooth in the graphic
The action trail of class animal during the experiment shows other data in experimentation in the table.Experimenter according to
When experimental data is analyzed, data obtained in combination with figure and table, cause workload larger, analysis efficiency is relatively low.
Invention content
The present invention provides a kind of rodent anxiety level test experiments track display method and device, current to solve
Anxiety level test experiments track display method caused by experimenter's heavy workload and anxiety level test experiments video point
Analyse the technical issues of efficiency is low.
In a first aspect, an embodiment of the present invention provides a kind of rodent anxiety level test experiments track display sides
Method, including：
Rodent experiment video is obtained, it is real in anxiety level test that the experiment video includes the rodent
Action trail in testing；
Rodent experiment video is analyzed according to preset time interval, obtains experiment picture and correspondence
The experiment moment；
Obtain the position of rodent pixel residing in the experiment picture, and during according to adjacent experiment
The variable quantity of the position of pixel described in the experiment picture at quarter residing for rodent, determines the rodent in institute
State the instantaneous relative velocity of pixel；
According to the position of rodent pixel residing in the experiment picture, draw the rodent and move
Movement locus of the object in the experiment video, and show the rodent in the pixel in the movement locus
Position at instantaneous relative velocity..
Second aspect, the embodiment of the present invention additionally provide a kind of rodent anxiety level test experiments track display dress
It puts, which includes：
Acquisition module, for obtaining rodent experiment video, the experiment video exists including the rodent
Action trail in anxiety level test experiments；
First determining module, for being analyzed according to preset time interval rodent experiment video,
Obtain experiment picture and corresponding experiment moment；
Second determining module, for obtaining the position of rodent pixel residing in the experiment picture
It puts, and the variable quantity of the position of the pixel according to the experiment picture at adjacent experiment moment residing for rodent, really
The fixed rodent is in the instantaneous relative velocity of the pixel；
Drafting module for the position according to rodent pixel residing in the experiment picture, is painted
Movement locus of the rodent in the experiment video is made, and shows that the rodent moves in the movement locus
Instantaneous relative velocity of the object at the position of the pixel.
The third aspect, the embodiment of the present invention additionally provide a kind of equipment, and the equipment includes：
One or more processors；
Memory, for storing one or more programs,
When one or more of programs are performed by one or more of processors so that one or more of processing
Device realizes rodent anxiety level test experiments track display method as described in relation to the first aspect.
Fourth aspect, the embodiment of the present invention additionally provide a kind of computer readable storage medium, are stored thereon with computer
Program realizes that rodent anxiety level test experiments track as described in relation to the first aspect is shown when the program is executed by processor
Show method.
Rodent anxiety level test experiments track display method and device provided in an embodiment of the present invention, by obtaining
Rodent is taken to test video, wherein, experiment video includes rodent the behavior rail in anxiety level test experiments
Mark is analyzed rodent experiment video according to preset time interval, when obtaining experiment picture and corresponding experiment
It carves, obtains the position of rodent pixel residing in picture is tested, and according to the experiment picture at adjacent experiment moment
The variable quantity of the position of pixel residing for middle rodent determines instantaneous relative velocity of the rodent in pixel,
According to the position of rodent pixel residing in picture is tested, movement of the rodent in video is tested is drawn
Track, and instantaneous relative velocity of the rodent at the position of pixel is shown in movement locus, realizing can be
The instantaneous phase at the position and the position of rodent pixel residing in picture is tested is shown in movement locus simultaneously
To speed, experimental result is intuitively analyzed convenient for experimenter, it will be in combination with figure and table compared to current experimenter
The mode of data is obtained, reduces the workload of experimenter, and then, it improves rodent anxiety level test experiments and regards
The analysis efficiency and precision of analysis of frequency.
Description of the drawings
Fig. 1 is spacious field experiment model figure；
Fig. 2 is elevated plus-maze test ideograph；
Fig. 3 is rodent anxiety level test experiments track display method embodiment one provided in an embodiment of the present invention
Flow diagram；
Fig. 4 is the schematic diagram of a kind of movement locus shown in embodiment illustrated in fig. 3；
Fig. 5 is rodent anxiety level test experiments track display method embodiment two provided in an embodiment of the present invention
Flow diagram；
Fig. 6 is rodent anxiety level test experiments track display device embodiment one provided in an embodiment of the present invention
Structure diagram；
Fig. 7 is rodent anxiety level test experiments track display device embodiment two provided in an embodiment of the present invention
Structure diagram；
Fig. 8 is a kind of structure diagram of terminal provided in an embodiment of the present invention.
Specific embodiment
The present invention is described in further detail with reference to the accompanying drawings and examples.It is understood that this place is retouched
The specific embodiment stated is used only for explaining the present invention rather than limitation of the invention.It also should be noted that in order to just
Part related to the present invention rather than entire infrastructure are illustrated only in description, attached drawing.
Fig. 3 is rodent anxiety level test experiments track display method embodiment one provided in an embodiment of the present invention
Flow diagram.This method can be performed by computer.As shown in figure 3, rodent provided in an embodiment of the present invention
Anxiety level test experiments track display method specifically comprises the following steps：
Step 301：Obtain rodent experiment video.
Wherein, experiment video includes rodent the action trail in anxiety level test experiments.
Specifically, the experiment in the embodiment of the present invention can be spacious field experiment and elevated plus-maze test.The two realities
Test the phobic anxiety state that can be used for investigating animal.
Referring to Fig.1 and 2, carrying out spacious field experiment, camera can be placed in the front of chest 11 or top, with
Acquire activity of the rodent in spacious field experiment.It, can be in closure arm and opening when carrying out elevated plus-maze test
Camera is placed in the top of arm crosspoint, to acquire activity of the rodent in elevated plus-maze test.It is of the invention real
It can be mouse to apply the rodent in example.
In step 301, can be computer from other equipment, for example, in video camera, obtain rodent experiment and regard
Frequency or computer receive the rodent experiment video that other equipment is sent.
The action trail of the rodent that rodent experiment video includes in an experiment, refers to that rodent moves
Object whithin a period of time, the behavior rail in the open arms and closure arm of the chest middle and high frame plus maze experiment of spacious field experiment
Mark.
Step 302：According to preset time interval to rodent experiment video analyze, obtain experiment picture and
The corresponding experiment moment.
Specifically, video player may be used in the embodiment of the present invention and play rodent experiment video.It is optional
Ground, the player in the embodiment of the present invention can be Adobe Premiere or Potplayer etc..
After analyzing rodent experiment video, multiple experiment pictures and each experiment picture can be obtained
The corresponding experiment moment.Involved " analysis " refers to that obtaining rodent at predetermined intervals moves in the embodiment of the present invention
Object tests the multi-picture of video.
Due to being that the video is analyzed at predetermined intervals, then in the multiple experiment pictures finally got,
The corresponding difference tested between the moment of multiple adjacent experiment pictures is equal.In other words, m-th of experiment picture is corresponding
The difference between the experiment moment corresponding with the m-1 experiment picture at moment is tested, when testing the corresponding experiment of picture with m+1
The difference carved between the experiment moment corresponding with m-th of experiment picture is equal.M is the integer more than 1.
It should be noted that the experiment moment in the embodiment of the present invention can be relative instant, that is, define rodent
Experiment video is 0 at the time of starting to play, at the time of the experiment moment is relative to 0 moment.
Step 303：The position of pixel of the rodent residing in picture test is obtained, and during according to adjacent experiment
The variable quantity of the position of pixel in the experiment picture at quarter residing for rodent, determines wink of the rodent in pixel
When relative velocity.
Specifically, after multiple experiment pictures are got, each experiment picture can be determined by image recognition technology
In face, the position of the pixel where rodent.For example, in a certain experiment picture, it is real to determine that rodent is located at
It tests in picture on the 8th pixel of the 5th row.It is alternatively possible to it is the position work with the pixel where rodent foreleg
For the pixel where rodent position or using the position of the pixel where rodent back leg as
The position of pixel where rodent can also be using the position of the pixel where rodent head as nibbling
The position of pixel where tooth class animal, can also be using the position of the pixel where rodent geometric center of gravity as
The position of pixel where rodent.
It, can be according to adjacent experiment after the position for determining rodent pixel residing in picture is tested
The variable quantity of the position of pixel in the experiment picture at moment residing for rodent, determines that rodent is each where it
The instantaneous relative velocity of a pixel.Since experiment picture is to test video to rodent with identical prefixed time interval
It is analyzed and is got, under the premise of the time determines, trail change amount is directly proportional to speed.In the embodiment of the present invention,
Rodent is characterized with the variable quantity of the position of the pixel residing for rodent in the experiment picture according to the adjacent experiment moment
The instantaneous relative velocity of animal pixel residing in picture is tested.
For example, the first experiment picture corresponded to for the first experiment moment, and the second experiment picture corresponded to for the second experiment moment, the
Three experiment pictures correspond to the third experiment moment, and the first experiment moment is adjacent with the second experiment moment, the second experiment moment and third
It is adjacent to test the moment.
In a kind of realization method：It is instantaneous opposite at the position of pixel in first experiment picture residing for rodent
Speed is the position of the pixel in the second experiment picture residing for rodent and rodent institute in the first experiment picture
The difference of the position of the pixel at place；It is instantaneous relatively fast at the position of pixel in second experiment picture residing for rodent
It spends and is, the position of the pixel in third experiment picture residing for rodent is tested in picture with second residing for rodent
Pixel position difference；Instantaneous relative velocity at the position of pixel in third experiment picture residing for rodent
For the position of the pixel in third experiment picture residing for rodent is tested in picture with second residing for rodent
The difference of the position of pixel.
In another realization method：Instantaneous phase at the position of pixel in first experiment picture residing for rodent
It is the position of the pixel in the second experiment picture residing for rodent and rodent in the first experiment picture to speed
The difference of the position of residing pixel；It is instantaneous opposite at the position of pixel in second experiment picture residing for rodent
Speed is the position of the pixel in third experiment picture residing for rodent and rodent institute in the first experiment picture
The difference of the position of the pixel at place divided by 2 value；At the position of pixel in third experiment picture residing for rodent
Instantaneous relative velocity is the position of the pixel in third experiment picture residing for rodent and grinding tooth in the second experiment picture
The difference of the position of pixel residing for class animal.The specific implementation process of the realization method will carry out specifically in embodiment two
It is bright.
In step 303, for each experiment picture, rodent institute in the experiment picture can be got
The position of the pixel at place, meanwhile, it gets in the experiment picture, during the position of pixel of the rodent residing for it
Instantaneous relative velocity.
Step 304：According to the position of rodent pixel residing in picture is tested, draw rodent and exist
The movement locus in video is tested, and shows that rodent is instantaneous relatively fast at the position of pixel in movement locus
Degree.
It specifically, can basis after the position for determining rodent pixel residing in picture is tested
Draw movement locus of the rodent in video is tested in these positions.In addition, in the embodiment of the present invention, for the ease of experiment
Intuitively observation experiment shows that rodent is instantaneous at the position of pixel to personnel in movement locus simultaneously as a result, going back
Relative velocity.
In embodiments of the present invention, after step 303, can also include the following steps：It determines multiple instantaneous relatively fast
Maximum instantaneous relative velocity in degree；By the quotient of the instantaneous relative velocity of each pixel and maximum instantaneous relative velocity, as
Instantaneous relative velocity after the normalization of each pixel.Instantaneous relative velocity is normalized that is, being equivalent to.Then phase
Ying Di, step 304 are specially according to the position of rodent pixel residing in picture is tested, and draw rodent
Movement locus in video is tested, and show rodent after the normalization at the position of pixel in movement locus
Instantaneous relative velocity.To instantaneous relative velocity normalize after show, instantaneous relative velocity can be adjusted to 0-1 it
Between, convenient for quickly being shown.
Rodent can be shown in pixel in movement locus by following several ways in the embodiment of the present invention
Position at instantaneous relative velocity：
The first realization method in a manner that text is shown, marks rodent in pixel in movement locus
Instantaneous relative velocity at position.It, can be by the instantaneous relative velocity of rodent in digital form in the realization method
It is labeled on movement locus.
Second of realization method shows identical shape, with shape at the position of the pixel residing for rodent
Instantaneous relative velocity of the different colours mark rodent at the position of pixel.It can be with not in the realization method
Different instantaneous relative velocities of the same characterization rodent at the position of pixel.For example, red orange malachite is blue
The instantaneous relative velocity that purple represents is less and less, i.e., the instantaneous relative velocity of red expression is maximum, the instantaneous relative velocity that purple represents
It is minimum.Fig. 4 is the schematic diagram of a kind of movement locus shown in embodiment illustrated in fig. 3.In Fig. 4, with different color (notes：
Fig. 4 adjustment is red, orange, yellow, green, indigo, azure, purple for gray-scale map, the in practice color of the color bar on the right of the every width figures of Fig. 4 from top to bottom,
The speed of expression is less and less) represent different instantaneous relative velocities.Instantaneous relative velocity in Fig. 4 has been carried out at normalization
It manages, between the ranging from 0-1 of the instantaneous relative velocity after normalization.
The movement locus of rodent in two groups of spacious field experiments and elevated plus-maze test is shown in Fig. 4.In Fig. 4
The first row be rodent in control group movement locus.The second row in Fig. 4 is the modeling carried out after anxiety modeling
The movement locus of rodent in group.Anxiety modeling refers to before the experiments were performed, making the rodent in modeling group
In anxiety state.Rodent it can be seen from Fig. 4 after anxiety modeling in spacious field experiment movement locus mostly on side
Edge region, central area movement is less, and speed is higher when central area moves.Compared with control group, show apparent
Anxiety-like behavior.In Elevated plus-maze, the rodent after anxiety modeling is living only in the closure arm for having barrier to block
It is dynamic, open arms activity is not almost arrived, and the rodent in control group goes to open arms exploratory behaviour.After control group
Rodent is compared, in modeling group after rodent show apparent anxiety-like behavior.
The third realization method shows identical color, with difference at the position of the pixel residing for rodent
Instantaneous relative velocity of the shape mark rodent at the position of pixel.It, can be with movement in the realization method
Different instantaneous relative velocities of the different shape characterization rodent of tracing point at the position of pixel in track.Example
Such as, it represents that instantaneous relative velocity is minimum with triangle, represents that instantaneous relative velocity is maximum with circle, between triangle and circle
Between shape in, the number of edges of shape is more, represents that instantaneous relative velocity is bigger.
Rodent anxiety level test experiments track display method provided in an embodiment of the present invention, by obtaining grinding tooth
Animal experimental video, wherein, experiment video includes rodent the action trail in anxiety level test experiments, according to
Preset time interval analyzes rodent experiment video, obtains experiment picture and corresponding experiment moment, obtains
The position of rodent pixel residing in picture is tested, and according to rodent in the adjacent experiment picture for testing the moment
The variable quantity of the position of pixel residing for animal determines instantaneous relative velocity of the rodent in pixel, according to grinding tooth
Movement locus of the rodent in video is tested is drawn in the position of class animal pixel residing in picture is tested, and
Instantaneous relative velocity of the rodent at the position of pixel is shown in movement locus, realizing can be in movement locus
In show instantaneous relative velocity at the position and the position of rodent pixel residing in picture test simultaneously, just
Experimental result is intuitively analyzed in experimenter, data are obtained in combination with figure and table compared to current experimenter
Mode reduces the workload of experimenter, and then, the analysis for improving rodent anxiety level test experiments video is imitated
Rate and precision of analysis.
Fig. 5 is rodent anxiety level test experiments track display method embodiment two provided in an embodiment of the present invention
Flow diagram.On the basis of embodiment shown in Fig. 3 of the embodiment of the present invention, to how to determine rodent in pixel
Instantaneous relative velocity be described in detail.In the embodiment of the present invention, the position of the pixel where rodent includes x-axis
Coordinate and y-axis coordinate.As shown in figure 5, the rodent anxiety level test experiments track that the present invention implements to provide is shown
Show that method includes the following steps：
Step 501：Obtain rodent experiment video.
Wherein, experiment video includes rodent the action trail in anxiety level test experiments.
Step 502：According to preset time interval to rodent experiment video analyze, obtain experiment picture and
The corresponding experiment moment.
Step 501 is similar with the realization process and technical principle of step 302 with step 301, step 502, no longer superfluous herein
It states.
Step 503：The position of rodent pixel residing in picture is tested is obtained, by multiple experiment pictures
In, the coordinate of the x-axis of rodent pixel residing in picture is tested, according to corresponding experiment moment from small to large
Sequence is arranged, and the set of the coordinate of the x-axis after arrangement is determined as the first matrix that size is N*1.
Wherein, the corresponding experiment picture of each element in the first matrix, N are the quantity of experiment picture obtained.
Specifically, the first matrix is column vector, and each row element corresponds to an experiment picture.Also, line number where element
Bigger, the experiment moment of corresponding experiment picture is bigger.
Step 504：It is tested multiple in pictures, the seat of the y-axis of rodent pixel residing in picture is tested
Mark is arranged, and the set of the coordinate of the y-axis after arrangement is determined as according to the sequence of corresponding experiment moment from small to large
Size is the second matrix of N*1.
Wherein, the corresponding experiment picture of each element in the second matrix.
Specifically, the second matrix is column vector, and each row element corresponds to an experiment picture.Also, line number where element
Bigger, the experiment moment of corresponding experiment picture is bigger.
It is understood that corresponding to the element of m rows in the element of the m rows in the first matrix, with the second matrix
Experiment picture the experiment moment it is identical.
Step 505：The gradient function of matrix labotstory MATLAB is called, determines the gradient matrix and the second square of the first matrix
The gradient matrix of battle array.
Wherein, the position in the gradient matrix of the first matrix be the value of the element of (1,1) be in the first matrix position for (2,
1) value of element and difference of the position for the value of the element of (1,1), the position in the gradient matrix of the first matrix are the member of (N, 1)
The value of element is that position is the value of element of (N, 1) and the difference of the value of element that position is (N-1,1), the first matrix in the first matrix
Gradient matrix in position be (i, 1) element value be in the first matrix position be (i-1,1) element value and position
The difference divided by 2 value of the value of element for (i+1,1), i are more than 1 and less than N.
Position in the gradient matrix of second matrix is that the value of the element of (1,1) is that position is (2,1) in the second matrix
The value of element and difference of the position for the value of the element of (1,1), the position in the gradient matrix of the second matrix is the element of (N, 1)
It is worth the value of the element for position in the second matrix is (N, 1) and the difference of the value of element that position is (N-1,1), the ladder of the second matrix
Degree matrix in position be (i, 1) element value be in the second matrix position be (i-1,1) element value and position be (i+
1,1) difference of the value of element divided by 2 value, i are more than 1 and less than N.
Specifically, the gradient matrix of the gradient matrix of the first matrix and the second matrix is the column vector that size is N*1.The
In the gradient matrix of the gradient matrix of one matrix and the second matrix in addition to the element of the first row and last column, position is (i, 1)
The value of element be that position is value and the position of element of (i-1,1) in original matrix (referring to the first matrix or the second matrix) be (i
+ 1,1) difference of the value of element divided by 2 value.The first row member in the gradient matrix of the gradient matrix of first matrix and the second matrix
The value of element is the value of the second row in original matrix and the difference of the value of the first row.The gradient of the gradient matrix of first matrix and the second matrix
The value of last column element is the value of last column in original matrix and the difference of the value of row second from the bottom in matrix.
Step 506：According to the gradient matrix of the first matrix and the gradient matrix of the second matrix, determine that rodent exists
The instantaneous relative velocity of all pixels point.
Specifically, it is the value of the element of (j, 1) and the gradient square of the second matrix by position in the gradient matrix of the first matrix
Average value of the position for the value of the element of (j, 1) in battle array is determined as the corresponding experiment of element that position in the first matrix is (j, 1)
In picture, the instantaneous relative velocity of the pixel residing for rodent.J is more than or equal to the 1, integer less than or equal to N.It will
Rodent of the average value of the instantaneous relative velocity of the x-axis of a certain pixel and the instantaneous relative velocity of y-axis as the pixel
The instantaneous relative velocity of animal.
Step 507：According to the position of rodent pixel residing in picture is tested, draw rodent and exist
The movement locus in video is tested, and shows that rodent is instantaneous relatively fast at the position of pixel in movement locus
Degree.
Step 507 is similar with the realization process and technical principle of step 304, and details are not described herein again.
The process of step 503- steps 507 is illustrated below in conjunction with matlab programs：
In above procedure, by the value of the position of the rodent determined pixel residing in picture is tested by
According to the facts test moment being sequentially written in the file of .slk or .csv forms from small to large.Pass through xlsread functions in matlab
Call this document.XPos is the first matrix in step 503, and yPos is the second matrix in step 504.Need what is illustrated
It is that smoothing processing has been carried out to initial data in the embodiment of the present invention.Again by the way that gradient functions is called to determine the first matrix
Gradient matrix xSpeed and the second matrix gradient matrix ySpeed.Mean functions are recalled, according to the gradient of the first matrix
The gradient matrix of matrix and the second matrix determines instantaneous relative velocity.SpeedMean is the matrix of instantaneous relative velocity composition.
After the instantaneous relative velocity of pixel where rodent is determined, according to the position of pixel and instantaneous phase
It maps to speed.Jet functions are the function for providing chromatographic-type.Abs functions act as taking absolute value.plot(xPos
(i),yPos(i),'*','Color',colcol(speedVal,:), ' MarkerSize', 3) in function, " * " represents final
The shape at movement locus midpoint drawn.By the end of plot functions, it is possible to show rodent in video is tested
Movement locus, and instantaneous relative velocity of the rodent at the position of pixel is shown in movement locus, if two
The instantaneous relative velocity of rodent is different at tracing point, then the color of the two tracing points is different.
The purpose of set (gca, ' xdir', ' reverse') function is overturning coordinate system.The purpose of overturning coordinate system is
In elevated plus-maze test, the placement location of open arms and closure arm is not always fixed, it is possible in certain experiment
Closure arm is located at the upper right corner and the lower left corner, and open arms are located at the upper left corner and the lower right corner.In in addition primary experiment, open arms are located at
The upper right corner and the lower left corner, closure arm are located at the upper left corner and the lower right corner.It, can be in placement location difference in order to keep seeking unity of standard
When, overturn coordinate system.
set(gca,'ytick',0:100000:6550)；set(gca,'yticklabel',
{″,″,″,″,″,″,″,″,″,″,″,″})；set(gca,'xtick',0:100104:9400)；set(gca,'
xticklabel',{″,″,″,″,″,″,″,″,″,″,″,″})；The purpose of this four sentences is removes in matlab in coordinate system
Scale value, the aesthetics for the movement locus drawn in the embodiment of the present invention can be increased by removing the scale value in coordinate system.
Rodent anxiety level test experiments track display method provided in an embodiment of the present invention, by obtaining grinding tooth
Animal experimental video, wherein, experiment video includes rodent the action trail in anxiety level test experiments, according to
Preset time interval analyzes rodent experiment video, obtains experiment picture and corresponding experiment moment, obtains
The position of rodent pixel residing in picture is tested, by multiple experiment pictures, rodent is in experiment picture
The coordinate of the x-axis of residing pixel in face is arranged, and will arrangement according to the sequence of corresponding experiment moment from small to large
The set of the coordinate of x-axis afterwards is determined as the first matrix that size is N*1, and by multiple experiment pictures, rodent is in reality
The coordinate of the y-axis of pixel residing in picture is tested, is arranged according to the sequence of corresponding experiment moment from small to large, and will
The set of the coordinate of y-axis after arrangement is determined as the second matrix that size is N*1, calls the gradient letter of matrix labotstory MATLAB
Number, determines the gradient matrix of the first matrix and the gradient matrix of the second matrix, according to the gradient matrix of the first matrix and second
The gradient matrix of matrix determines instantaneous relative velocity of the rodent in all pixels point, is being tested according to rodent
Movement locus of the rodent in video is tested is drawn, and in movement locus in the position of residing pixel in picture
Show instantaneous relative velocity of the rodent at the position of pixel, realizing can be determined instantaneously by matlab programs
Relative velocity, so as to further improve the efficiency and accuracy of anxiety level test experiments video analysis.
Fig. 6 is rodent anxiety level test experiments track display device embodiment one provided in an embodiment of the present invention
Structure diagram.As shown in fig. 6, display dress in rodent anxiety level test experiments track provided in an embodiment of the present invention
It puts including following module：Acquisition module 61, the first determining module 62, the second determining module 63 and drafting module 64.
Acquisition module 61, for obtaining rodent experiment video.
Wherein, experiment video includes rodent the action trail in anxiety level test experiments.
First determining module 62 for being analyzed according to preset time interval rodent experiment video, obtains
To experiment picture and corresponding experiment moment.
Second determining module 63, for obtaining the position of rodent pixel residing in picture is tested, and root
According to the variable quantity of the position of the pixel residing for rodent in the experiment picture at adjacent experiment moment, rodent is determined
In the instantaneous relative velocity of pixel.
Drafting module 64 for the position according to rodent pixel residing in picture is tested, draws grinding tooth
Movement locus of the class animal in video is tested, and wink of the rodent at the position of pixel is shown in movement locus
When relative velocity.
Optionally, the side of instantaneous relative velocity of the rodent at the position of pixel is shown in movement locus
Face, drafting module 64 are specifically used for：In a manner that text is shown, rodent is marked in movement locus in the position of pixel
Put the instantaneous relative velocity at place；Alternatively, identical shape is shown at the position of the pixel residing for rodent, with shape
Instantaneous relative velocity of the different colours mark rodent at the position of pixel；Alternatively, residing for rodent
Pixel position at show identical color, mark wink of the rodent at the position of pixel in different shapes
When relative velocity.
Optionally, in the embodiment of the present invention, which further includes third determining module (not shown).Third determines mould
Block is used for：Determine the maximum instantaneous relative velocity in multiple instantaneous relative velocities；By the instantaneous relative velocity of each pixel with
The quotient of maximum instantaneous relative velocity, as the instantaneous relative velocity after the normalization of each pixel.Correspondingly, drafting module
64 are specifically used for：Show that rodent is instantaneous relatively fast after the normalization at the position of pixel in movement locus
Degree.
The rodent anxiety level test experiments track display device that the embodiment of the present invention is provided can perform Fig. 3 institutes
The rodent anxiety level test experiments track display method provided in embodiment is provided, has the corresponding function of execution method
Module and advantageous effect.
Fig. 7 is rodent anxiety level test experiments track display device embodiment two provided in an embodiment of the present invention
Structure diagram.On the basis of embodiment shown in Fig. 6 of the embodiment of the present invention, one is made to the concrete composition of the second determining module
It is described in detail.As shown in fig. 7, in the embodiment of the present invention, the second determining module 63 includes：First determination sub-module 631, second is really
Stator modules 632,633 and the 4th determination sub-module 634 of third determination sub-module.
First determination sub-module 631, for being tested multiple in pictures, rodent picture residing in picture is tested
The coordinate of the x-axis of vegetarian refreshments is arranged according to corresponding experiment moment sequence from small to large, and by the seat of the x-axis after arrangement
Target set is determined as the first matrix that size is N*1.
Wherein, the corresponding experiment picture of each element in the first matrix, N are the quantity of experiment picture obtained.
Second determination sub-module 632, for being tested multiple in pictures, rodent picture residing in picture is tested
The coordinate of the y-axis of vegetarian refreshments is arranged according to corresponding experiment moment sequence from small to large, and by the seat of the y-axis after arrangement
Target set is determined as the second matrix that size is N*1.
Wherein, the corresponding experiment picture of each element in the second matrix.
Third determination sub-module 633 for calling the gradient function of matrix labotstory MATLAB, determines the ladder of the first matrix
Spend the gradient matrix of matrix and the second matrix.
Wherein, the position in the gradient matrix of the first matrix be the value of the element of (1,1) be in the first matrix position for (2,
1) value of element and difference of the position for the value of the element of (1,1), the position in the gradient matrix of the first matrix are the member of (N, 1)
The value of element is that position is the value of element of (N, 1) and the difference of the value of element that position is (N-1,1), the first matrix in the first matrix
Gradient matrix in position be (i, 1) element value be in the first matrix position be (i-1,1) element value and position
The difference divided by 2 value of the value of element for (i+1,1), i are more than 1 and less than N.
Position in the gradient matrix of second matrix is that the value of the element of (1,1) is that position is (2,1) in the second matrix
The value of element and difference of the position for the value of the element of (1,1), the position in the gradient matrix of the second matrix is the element of (N, 1)
It is worth the value of the element for position in the second matrix is (N, 1) and the difference of the value of element that position is (N-1,1), the ladder of the second matrix
Degree matrix in position be (i, 1) element value be in the second matrix position be (i-1,1) element value and position be (i+
1,1) difference of the value of element divided by 2 value.
4th determination sub-module 634, for the gradient matrix according to the first matrix and the gradient matrix of the second matrix, really
Determine instantaneous relative velocity of the rodent in all pixels point.
In a kind of realization method, the 4th determination sub-module 634 specifically for being by position in the gradient matrix of the first matrix
The value of the element of (j, 1) and average value of the position in the gradient matrix of the second matrix for the value of the element of (j, 1), are determined as first
Position is the instantaneous relative velocity of the pixel residing for rodent in the corresponding experiment picture of element of (j, 1) in matrix,
J is more than or equal to the 1, integer less than or equal to N.
The rodent anxiety level test experiments track display device that the embodiment of the present invention is provided can perform Fig. 5 institutes
The rodent anxiety level test experiments track display method provided in embodiment is provided, has the corresponding function of execution method
Module and advantageous effect.
Fig. 8 is a kind of structure diagram of terminal provided in an embodiment of the present invention.As shown in figure 8, the equipment includes processing
Device 70, memory 71 and output device 73.Optionally, input unit 72 can also be included.The quantity of processor 70 can in equipment
To be one or more, in Fig. 8 by taking a processor 70 as an example；Processor 70, memory 71,72 and of input unit in equipment
Output device 73 can be connected by bus or other modes, in Fig. 8 for being connected by bus.
Memory 71 is used as a kind of computer readable storage medium, and journey is can perform available for storage software program, computer
Sequence and module, such as the corresponding module of rodent anxiety level test experiments track display method in the embodiment of the present invention
(for example, acquisition module 61, the first determining module 62 in rodent anxiety level test experiments track display device,
Two determining modules 63 and drafting module 64).Processor 70 by operation be stored in software program in memory 71, instruction and
Module so as to perform the various function application of equipment and data processing, that is, realizes that above-mentioned rodent anxiety level is surveyed
Try experimental traces display methods.
Memory 71 can mainly include storing program area and storage data field, wherein, storing program area can store operation system
Application program needed for system, at least one function；Storage data field can be stored uses created data etc. according to terminal.This
Outside, memory 71 can include high-speed random access memory, can also include nonvolatile memory, for example, at least a magnetic
Disk storage device, flush memory device or other non-volatile solid state memory parts.In some instances, memory 71 can be further
Including relative to the remotely located memory of processor 70, these remote memories can pass through network connection to equipment.It is above-mentioned
The example of network includes but not limited to internet, intranet, LAN, mobile radio communication and combinations thereof.
Input unit 72 can be used for receiving input number or character information and generate with the user setting of equipment and
The related key signals input of function control.Output device 73 may include that display screen etc. shows equipment.
The embodiment of the present invention also provides a kind of storage medium for including computer executable instructions, computer executable instructions
It is used to perform a kind of rodent anxiety level test experiments track display method, the party when being performed by computer processor
Method includes：
Rodent experiment video is obtained, experiment video includes rodent the row in anxiety level test experiments
For track；
Rodent experiment video is analyzed according to preset time interval, obtains experiment picture and corresponding reality
Test the moment；
The position of rodent pixel residing in picture is tested is obtained, and according to the experiment at adjacent experiment moment
The variable quantity of the position of pixel in picture residing for rodent determines rodent in the instantaneous relatively fast of pixel
Degree；
According to the position of rodent pixel residing in picture is tested, rodent is drawn in experiment video
In movement locus, and instantaneous relative velocity of the rodent at the position of pixel is shown in movement locus.
Certainly, a kind of storage medium for including computer executable instructions that the embodiment of the present invention is provided, computer
Executable instruction is not limited to method as above operation, and it is burnt that the rodent that any embodiment of the present invention is provided can also be performed
Consider the relevant operation in horizontal checkout experimental traces display methods
By the description above with respect to embodiment, it is apparent to those skilled in the art that, the present invention
It can be realized by software and required common hardware, naturally it is also possible to which by hardware realization, but the former is more in many cases
Good embodiment.Based on such understanding, what technical scheme of the present invention substantially in other words contributed to the prior art
Part can be embodied in the form of software product, which can be stored in computer readable storage medium
In, floppy disk, read-only memory (Read-Only Memory, ROM), random access memory (Random such as computer
Access Memory, RAM), flash memory (FLASH), hard disk or CD etc., including some instructions with so that a computer is set
The method that standby (can be personal computer, server or the network equipment etc.) performs each embodiment of the present invention.
It is worth noting that, in the embodiment of above-mentioned searcher, included each unit and module are only according to work(
Energy logic is divided, but be not limited to above-mentioned division, as long as corresponding function can be realized；In addition, each work(
The specific name of energy unit is also only to facilitate mutually distinguish, the protection domain being not intended to restrict the invention.
Note that it above are only presently preferred embodiments of the present invention and institute's application technology principle.It will be appreciated by those skilled in the art that
The present invention is not limited to specific embodiment here, various apparent variations, again can be carried out for a person skilled in the art
Adjustment and replacement are without departing from protection scope of the present invention.Therefore, although the present invention has been carried out by above example compared with
For detailed description, but the present invention is not limited only to above example, without departing from the inventive concept, can be with
Including other more equivalent embodiments, and the scope of the present invention is determined by scope of the appended claims.
Claims (10)
1. a kind of rodent anxiety level test experiments track display method, which is characterized in that including：
Rodent experiment video is obtained, the experiment video includes the rodent in anxiety level test experiments
Action trail；
Rodent experiment video is analyzed according to preset time interval, obtains experiment picture and corresponding reality
Test the moment；
The position of rodent pixel residing in the experiment picture is obtained, and according to the adjacent experiment moment
The variable quantity of the position of the pixel described in picture residing for rodent is tested, determines the rodent in the picture
The instantaneous relative velocity of vegetarian refreshments；
According to the position of rodent pixel residing in the experiment picture, draw the rodent and exist
Movement locus in the experiment video, and show the rodent in the position of the pixel in the movement locus
Put the instantaneous relative velocity at place.
2. according to the method described in claim 1, it is characterized in that, described show that the rodent moves in the movement locus
Instantaneous relative velocity of the object at the position of the pixel, including：
In a manner that text is shown, the rodent is marked in the movement locus at the position of the pixel
Instantaneous relative velocity；Alternatively,
Identical shape is shown at the position of the pixel residing for the rodent, with the different colours mark of the shape
Note instantaneous relative velocity of the rodent at the position of the pixel；Alternatively,
Identical color is shown at the position of the pixel residing for the rodent, is nibbled described in mark in different shapes
Instantaneous relative velocity of the tooth class animal at the position of the pixel.
3. method according to claim 1 or 2, which is characterized in that the position of the pixel includes the coordinate and y of x-axis
The coordinate of axis；
The variable quantity of the position of pixel described in the experiment picture according to adjacent moment residing for rodent, determines
The rodent the pixel instantaneous relative velocity, including：
By in multiple experiment pictures, the coordinate of the x-axis of rodent pixel residing in the experiment picture is pressed
It is arranged, and the set of the coordinate of the x-axis after arrangement is determined as size according to the sequence of corresponding experiment moment from small to large
The first matrix for N*1；Wherein, the corresponding experiment picture of each element in first matrix, N is the experiment obtained
The quantity of picture；
By in the multiple experiment picture, the rodent is in the seat of the y-axis for testing pixel residing in picture
Mark is arranged, and the set of the coordinate of the y-axis after arrangement is determined as according to the sequence of corresponding experiment moment from small to large
Size is the second matrix of N*1；Wherein, the corresponding experiment picture of each element in second matrix；
It calls the gradient function of matrix labotstory MATLAB, determines the gradient matrix of first matrix and second matrix
Gradient matrix；Wherein, the position in the gradient matrix of first matrix is the value of the element of (1,1) in first matrix
The difference of the value for the element that position is the value of the element of (2,1) and position is (1,1), the position in the gradient matrix of first matrix
The value for being set to the element of (N, 1) be in first matrix position be (N, 1) element value and position be (N-1,1) element
Value difference, the position in the gradient matrix of first matrix is that the value of the element of (i, 1) is position in first matrix
The value of element for (i-1,1) and difference divided by 2 value of the position for the value of the element of (i+1,1), i are more than 1 and less than N；It is described
Position in the gradient matrix of second matrix is that the value of the element of (1,1) is the element that position is (2,1) in second matrix
Difference for the value of the element of (1,1) of value and position, the position in the gradient matrix of second matrix is the element of (N, 1)
It is worth the value for element that position in second matrix is (N, 1) and the difference of the value of element that position is (N-1,1), described second
Position in the gradient matrix of matrix is that the value of the element of (i, 1) is the element that position is (i-1,1) in second matrix
The difference divided by 2 value of value and position for the value of the element of (i+1,1)；
According to the gradient matrix of first matrix and the gradient matrix of second matrix, determine that the rodent exists
The instantaneous relative velocity of all pixels.
4. the according to the method described in claim 3, it is characterized in that, gradient matrix and institute according to first matrix
The gradient matrix of the second matrix is stated, determines instantaneous relative velocity of the rodent in all pixels, including：
By position in the gradient matrix of first matrix in the value of element of (j, 1) and the gradient matrix of second matrix
Average value of the position for the value of the element of (j, 1) is determined as the corresponding experiment picture of element that position in the first matrix is (j, 1)
In, the instantaneous relative velocity of the pixel residing for the rodent, j is more than or equal to the 1, integer less than or equal to N.
5. the according to the method described in claim 3, it is characterized in that, gradient matrix and institute according to first matrix
The gradient matrix of the second matrix is stated, determines the rodent after the instantaneous relative velocity of all pixels, institute
The method of stating further includes：
Determine the maximum instantaneous relative velocity in the multiple instantaneous relative velocity；
By the quotient of the instantaneous relative velocity of each pixel and the maximum instantaneous relative velocity, as each pixel
Instantaneous relative velocity after the normalization of point；
The instantaneous relative velocity that the rodent is shown in the movement locus at the position of the pixel,
Including：
Instantaneous phase of the rodent after the normalization at the position of the pixel is shown in the movement locus
To speed.
6. a kind of rodent anxiety level test experiments track display device, which is characterized in that including：
Acquisition module, for obtaining rodent experiment video, the experiment video includes the rodent in anxiety
Action trail in horizontal checkout experiment；
First determining module for being analyzed according to preset time interval rodent experiment video, obtains
Test picture and corresponding experiment moment；
Second determining module, for obtaining the position of rodent pixel residing in the experiment picture, and
The variable quantity of the position of pixel according to the experiment picture at adjacent experiment moment residing for rodent determines described
Rodent is in the instantaneous relative velocity of the pixel；
Drafting module for the position according to rodent pixel residing in the experiment picture, draws institute
Movement locus of the rodent in the experiment video is stated, and shows that the rodent exists in the movement locus
Instantaneous relative velocity at the position of the pixel.
7. device according to claim 6, which is characterized in that the drafting module is specifically used for：
In a manner that text is shown, the rodent is marked in the movement locus at the position of the pixel
Instantaneous relative velocity；Alternatively,
Identical shape is shown at the position of the pixel residing for the rodent, with the different colours mark of the shape
Note instantaneous relative velocity of the rodent at the position of the pixel；Alternatively,
Identical color is shown at the position of the pixel residing for the rodent, is nibbled described in mark in different shapes
Instantaneous relative velocity of the tooth class animal at the position of the pixel.
8. the device described according to claim 6 or 7, which is characterized in that the position of the pixel includes the coordinate and y of x-axis
The coordinate of axis；
Second determining module specifically includes：
First determination sub-module, for by multiple experiment pictures, the rodent is residing in the experiment picture
The coordinate of the x-axis of pixel is arranged according to corresponding experiment moment sequence from small to large, and by the x-axis after arrangement
The set of coordinate is determined as the first matrix that size is N*1；Wherein, each element in first matrix corresponds to a reality
Picture is tested, N is the quantity of experiment picture obtained；
Second determination sub-module, for by the multiple experiment picture, the rodent to be in the institute in testing picture
The coordinate of the y-axis of the pixel at place is arranged according to corresponding experiment moment sequence from small to large, and by the y after arrangement
The set of the coordinate of axis is determined as the second matrix that size is N*1；Wherein, each element in second matrix corresponding one
A experiment picture；
Third determination sub-module for calling the gradient function of matrix labotstory MATLAB, determines the gradient of first matrix
The gradient matrix of matrix and second matrix；Wherein, the position in the gradient matrix of first matrix is the element of (1,1)
Value be in first matrix position be (2,1) element value and position be (1,1) element value difference, described first
Position in the gradient matrix of matrix be the value of the element of (N, 1) be in first matrix position be (N, 1) element value
With difference of the position for the value of the element of (N-1,1), value of the position for the element of (i, 1) in the gradient matrix of first matrix
For the value of element that position in first matrix is (i-1,1) and the difference of the value of element that position is (i+1,1) divided by 2
Value, i are more than 1 and less than N；Position in the gradient matrix of second matrix is that the value of the element of (1,1) is second square
The difference of the value for the element that position be the value of the element of (2,1) in battle array and position is (1,1), in the gradient matrix of second matrix
Position be the value of element of (N, 1) be that position is value and the position of element of (N, 1) in second matrix be (N-1,1)
The difference of the value of element, the position in the gradient matrix of second matrix are that the value of the element of (i, 1) is in second matrix
The difference divided by 2 value of the value of element that position is the value of the element of (i-1,1) and position is (i+1,1)；
4th determination sub-module, for the gradient matrix according to first matrix and the gradient matrix of second matrix,
Determine instantaneous relative velocity of the rodent in all pixels.
9. a kind of equipment, which is characterized in that the equipment includes：
One or more processors；
Memory, for storing one or more programs,
When one or more of programs are performed by one or more of processors so that one or more of processors are real
The now rodent anxiety level test experiments track display method as described in any in claim 1-5.
10. a kind of computer readable storage medium, is stored thereon with computer program, which is characterized in that the program is by processor
The rodent anxiety level test experiments track display method as described in any in claim 1-5 is realized during execution.
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