CN108159455B - Paper money biological sterilization device and sterilization method thereof - Google Patents
Paper money biological sterilization device and sterilization method thereof Download PDFInfo
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- CN108159455B CN108159455B CN201810181316.3A CN201810181316A CN108159455B CN 108159455 B CN108159455 B CN 108159455B CN 201810181316 A CN201810181316 A CN 201810181316A CN 108159455 B CN108159455 B CN 108159455B
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- 230000001954 sterilising effect Effects 0.000 title claims abstract description 196
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 112
- 238000000034 method Methods 0.000 title claims abstract description 22
- 239000012528 membrane Substances 0.000 claims abstract description 107
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 105
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 105
- 238000000018 DNA microarray Methods 0.000 claims abstract description 101
- 241000894006 Bacteria Species 0.000 claims abstract description 62
- 239000000523 sample Substances 0.000 claims description 58
- 241000700605 Viruses Species 0.000 claims description 21
- 230000001580 bacterial effect Effects 0.000 claims description 21
- 238000010438 heat treatment Methods 0.000 claims description 9
- 230000009172 bursting Effects 0.000 claims description 8
- 238000005054 agglomeration Methods 0.000 claims description 7
- 230000002776 aggregation Effects 0.000 claims description 7
- 241000700721 Hepatitis B virus Species 0.000 claims description 6
- 229910052573 porcelain Inorganic materials 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 4
- 238000005096 rolling process Methods 0.000 claims description 4
- 230000009471 action Effects 0.000 claims description 3
- 238000007886 magnetic bead extraction Methods 0.000 claims description 3
- 238000013518 transcription Methods 0.000 claims description 3
- 230000035897 transcription Effects 0.000 claims description 3
- 201000008827 tuberculosis Diseases 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 abstract description 15
- 230000009286 beneficial effect Effects 0.000 abstract description 4
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- 108090000623 proteins and genes Proteins 0.000 description 20
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- 239000011248 coating agent Substances 0.000 description 6
- 238000000576 coating method Methods 0.000 description 6
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- 241000193830 Bacillus <bacterium> Species 0.000 description 4
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- 108010052285 Membrane Proteins Proteins 0.000 description 2
- 101710163270 Nuclease Proteins 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
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- ZJYYHGLJYGJLLN-UHFFFAOYSA-N guanidinium thiocyanate Chemical compound SC#N.NC(N)=N ZJYYHGLJYGJLLN-UHFFFAOYSA-N 0.000 description 2
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/04—Heat
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- Health & Medical Sciences (AREA)
- Epidemiology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Filtering Materials (AREA)
Abstract
The invention provides a paper currency biological sterilization device and a sterilization method thereof. The biological sterilization device comprises a die body, a convex lens, a first sterilization lamp, a second sterilization lamp, a first nitrocellulose membrane DNA chip cover and a second nitrocellulose membrane DNA chip cover. The sterilizing device adopts three technologies of a DNA biotechnology, an electrothermal technology and a photoelectric technology to realize the full sterilization coverage of paper money in a centralized way, improves the sterilization performance of the paper money, is beneficial to the environmental protection of the paper money and the health protection of people; the method can reduce the back cage and destruction of incomplete and stained paper money in the circulation process; the method is beneficial to the financial institutions to maintain the authority of the paper currency by mainly sterilizing the bacteria of the paper currency.
Description
Technical Field
The invention belongs to the technical field of biology, and relates to a paper currency biological sterilization device and a sterilization method thereof.
Background
At present, the circulation speed of paper money is increased, 70 hundred million people worldwide are in contact with the paper money every day, and the paper money has more contact people in the circulation process and complicated layers, so that the paper money becomes an important path for pathogenic transmission. According to statistics, the average bacterial content of each RMB is 17.8 ten thousands, wherein: the positive detection rate of colibacillus, staphylococcus aureus and bacillus cereus in colibacillus group is 65.6%, 45.9%, 41.7% and 19.8%, especially a large number of virus bacteria are attached to the anti-counterfeiting point of paper money, so that the sterilization of the existing financial institution machine is difficult. At present, no sterilizing device is available for ATM machines, banknote counting machines, banknote bundling machines and other direct contact banknote machines, and part of machines are sterilized by spraying sterilizing water regularly. The paper money sterilizing process is too simple, and because virus and bacteria like high temperature and humidity, and virus and bacteria are gathered and rotated in opposite directions between individuals, the temperature of the closed space can rise, and the sterilizing water is liquid which can not completely kill the virus and bacteria on the front and the back sides of a single paper money on a machine.
Disclosure of Invention
In order to overcome the defect that effective sterilization equipment and technology are not available in the aspect of banknote sterilization at present, the invention aims to provide a banknote biological sterilization device and a banknote biological sterilization method so as to improve the banknote sterilization performance and inhibit virus transmission.
The aim of the invention is achieved by the following technical scheme:
in one aspect, the present invention provides a banknote biological sterilization apparatus, the biological sterilization apparatus comprising a mold body, a convex lens, a first sterilization lamp, a second sterilization lamp, a first nitrocellulose membrane DNA chip cover, and a second nitrocellulose membrane DNA chip cover;
the die body is of a rectangular box structure, and one convex lens is respectively distributed on the four inner side walls of the box body;
two symmetrical horizontal slide ways are fixedly arranged on the two symmetrical convex lenses, and are parallel to the inner side walls of the box bodies corresponding to the two symmetrical convex lenses; the first sterilizing lamp and the second sterilizing lamp are long-tube-shaped, the length direction of the first sterilizing lamp and the second sterilizing lamp are respectively perpendicular to the slide ways, two ends of the first sterilizing lamp and two ends of the second sterilizing lamp are respectively and movably connected with two horizontal slide ways, and the slide ways, the first sterilizing lamp and the second sterilizing lamp are respectively and electrically connected with a photoelectric driving device; when the paper money biological sterilization device operates, the photoelectric driving device is started to drive the first sterilization lamp and the second sterilization lamp to roll towards the middle part of the slideway along the two ends of the slideway to gather together oppositely and separate oppositely;
a straight-strip-shaped lamp post door frame is arranged in the middle of a slideway where the first sterilizing lamp and the second sterilizing lamp are gathered in opposite directions, the lamp post door frame is perpendicular to the slideway and parallel to the first sterilizing lamp and the second sterilizing lamp, a gap perpendicular to the slideway and parallel to the first sterilizing lamp and the second sterilizing lamp is arranged in the middle of the lamp post door frame, and the lower end of the gap is communicated with a bacterial hopper; when the paper money biological sterilization device operates, the first sterilization lamp and the second sterilization lamp gather virus bacteria to the lamp post door frame for sterilization, and the virus bacteria are sent to the bacteria hopper for collection through a gap of the lamp post door frame;
the first nitrocellulose membrane DNA chip cover and the second nitrocellulose membrane DNA chip cover are respectively sleeved on the outer sides of the first sterilizing lamp and the second sterilizing lamp.
In the banknote biological sterilization device, the photoelectric driving device is used for starting the first sterilization lamp and the second sterilization lamp to form photocurrent and ultraviolet rays; meanwhile, the slide way is driven to realize the back and forth rolling of the first sterilizing lamp and the second sterilizing lamp on the slide way.
In the banknote biological sterilization device, preferably, the lampwicks of the first sterilization lamp and the second sterilization lamp are LED lampwicks, ultraviolet light is filled in the lamp tube, and two ends of the lamp tube are sealed by a porcelain lamp cap and are electrically connected with a photoelectric driving device;
more preferably, the ultraviolet light has a wavelength of 250-260nm.
In the banknote biological sterilization apparatus, preferably, the first nitrocellulose membrane DNA chip cover and/or the second nitrocellulose membrane DNA chip cover are/is fixedly sleeved outside the first sterilization lamp and the second sterilization lamp by bolts, and the annular gap distance is 0.2cm, but the banknote biological sterilization apparatus is not limited thereto; the "annular space" refers to the distance from the outer side wall of the germicidal lamp to the inner side wall of the nitrocellulose membrane DNA chip cover.
In the banknote biological sterilization apparatus, preferably, the first nitrocellulose membrane DNA chip cover or the second nitrocellulose membrane DNA chip cover is prepared by:
extracting purified DNA from bursting bacteria by using a nano magnetic bead extraction method, preparing a rotary probe according to a bacteria rotation agglomeration rule, fixing the rotary probe on a nitrocellulose membrane by transcription, and rotating the obtained product into a cylinder shape to prepare a first nitrocellulose membrane DNA chip cover or a second nitrocellulose membrane DNA chip cover, wherein the probe is positioned on the outer side wall of the first nitrocellulose membrane DNA chip cover or the second nitrocellulose membrane DNA chip cover.
In the banknote biological sterilization apparatus, preferably, the outer side wall of the first nitrocellulose membrane DNA chip cover or the second nitrocellulose membrane DNA chip cover has a rugged structure, the raised portion is provided with a probe, and the recessed portion is provided with a light lamp hole (illumination hole); the probe is a balanced spike rod type probe.
In the banknote biological sterilization apparatus, the number of probes on the first nitrocellulose membrane DNA chip cover or the second nitrocellulose membrane DNA chip cover is preferably 120, but not limited thereto. The gene microarray formed by the probe arrangement is a DNA biological gene chip.
In the above-described banknote biological sterilization apparatus, preferably, the pop-up bacteria may include tuberculosis bacteria and/or hepatitis b virus bacteria, and the like. The tubercle bacillus and hepatitis B virus bacteria are commercially available.
In the banknote biological sterilization apparatus, preferably, a banknote inlet and outlet is provided on a side wall of the mold body, and the banknote inlet and outlet is opened upward at an inclined plane of 30 degrees (not limited thereto) with respect to a lower end surface of the mold body.
In the banknote biological sterilization device, preferably, the lower end opening of the die body is communicated with and connected with a banknote handling box with banknote twisting function; the banknote enters the banknote handling box through the banknote inlet and outlet.
In the banknote biological sterilization apparatus described above, the banknote handling machine may be an ATM banknote handling machine, a banknote counter, a banknote bundling machine, or the like, as required.
In the banknote biological sterilization apparatus, it is preferable that the banknote distance between the banknote inlet and outlet and the first nitrocellulose membrane DNA chip cover and the second nitrocellulose membrane DNA chip cover is 2cm when the banknote operation box twists banknotes, but the banknote biological sterilization apparatus is not limited thereto.
In the banknote biological sterilization apparatus, preferably, a U-disk socket is provided on a side wall of the mold body, and is used for electrically connecting the banknote operation box. The paper money operation box can automatically supply power to operate, and the paper money operation box can also be electrically connected with the USB flash disk socket by connecting the USB flash disk socket through the photoelectric driving device to supply power to the paper money operation box.
In the banknote biological sterilization apparatus, preferably, the upper end of the die body is electrically connected with an operating system box. The method is used for realizing the integration of ATM sterilization and money taking operation.
In the banknote biological sterilization apparatus, the mold body is preferably 17cm long, 5cm wide, and 4cm high, but is not limited thereto.
In the banknote biological sterilization apparatus, the convex curvature of the convex lens is preferably 15 degrees, but not limited thereto.
The manufacturing method of the paper currency biological sterilization device comprises the following steps:
first, a germicidal lamp (LED lamp core and ultraviolet light)
In the banknote biological sterilization device, a tube of a sterilization lamp is made of a quartz glass material into a hollow tube; the lamp core in the lamp tube adopts an LED lamp core filament, fluorescent powder is arranged on the inner wall of the lamp tube, the lamp tube uses a hot cathode to discharge mercury vapor under low pressure, ultraviolet light is injected into the lamp tube, the lamp tube is sealed by a porcelain lamp cap, and the wavelength of the ultraviolet light is 250-260 nm; the diameter of the lamp tube was set to 0.5cm.
Second step, preparing nitrocellulose membrane DNA chip cover
The nitrocellulose membrane is adopted to prepare a nitrocellulose membrane DNA chip cover, and 120 balanced convex spike needle bar probes are arranged outside the chip cover; the chip cover and the sterilizing lamp are sleeved and fixed by bolts, and the distance between the chip cover and the sterilizing lamp is 0.2cm.
And (3) preparing a DNA biological gene chip. Extracting molecular cells of pop bacteria such as tubercle bacillus and hepatitis B virus bacteria. Decomposing bacterial gene protein molecules and extracting bursting bacterial molecule genes. The probe made of virus carrying burst gene protein is solidified on nitrocellulose membrane to make DNA chip, and in situ hybridization is carried out. After signal detection and result analysis, DNA is cloned and duplicated after heating and drying to prepare a DNA biological gene chip probe, and the probe is transcribed on a nitrocellulose membrane lampshade. The gene microarray formed by the probe arrangement is a DNA biological gene chip.
The specific steps for manufacturing the probe are as follows:
1. 0.5-1. Mu.L of fresh protein was extracted from the burster bacteria and DNA samples sufficient for N-fold successful amplification were extracted.
2. Firstly, preparing 5-8nm magnetic nano particles, so that the magnetic nano particles have very high magnetic field response capability and superparamagnetic property, and nano regulation and control can be realized by changing an external magnetic field;
3. coating the magnetic nano particles by using a coating technology;
4. by utilizing a surface chemical modification technology, functional groups which can specifically act on DNA are connected, and the modified functional groups control the reversible adsorption property of the DNA;
5. the accurate amount of DNA is obtained by surface modification and control of the conditions such as the ionic strength, the pH value and the like in the solution.
6. Extracting and purifying DNA, using strong protein denaturants such as guanidine thiocyanate, destroying cell membrane and nuclear membrane proteins, releasing DNA, and inactivating nuclease;
7. adding magnetic beads, and carrying out specific adsorption on DNA through chemical groups on the surface;
8. separating the magnetic particles from the liquid and recovering the particles;
9. the adsorbed DNA was eluted with pure water or TE, and the DNA was dissociated from the magnetic beads in the solution to re-elute the DNA.
10. And (3) preparing a new probe, coating the DNA magnetic nano particles for the second time, preparing a rotary probe according to a bacterial rotation agglomeration rule by using an ink-jet printer or an artificial robot mode, and transferring and fixing the rotary probe on a nitrocellulose membrane cover.
Third, manufacturing a convex lens reflecting device
The periphery of the inner wall of the die is provided with reflective convex lenses, the four-sided convex lenses of the die and the inner wall of the die form an oblique angle (convex radian) of 15 degrees, and bacteria are accelerated to form clusters through photocurrent pairs in a photoelectric driving device. Meanwhile, the inner side wall of the nitrocellulose membrane DNA chip cover is heated to 80 ℃ through photocurrent in the photoelectric driving device, the irradiated convex lens is reflected to the nitrocellulose membrane DNA chip cover, and a plurality of convex spike needle bar probes and concave pinholes are arranged on the outer side wall of the nitrocellulose membrane DNA chip cover.
Fourth step, convex wolf tooth needle bar probe and concave needle hole
The current heating device and the lens reflecting device are used for heating, illuminating and pushing the probe to release the trapping and killing rotary cluster bursting factors to kill different bacteria on paper money.
In the banknote biological sterilization device, the diameter of an opening of the probe on the surface of banknote virus is 0.01-0.02mm.
In the banknote biological sterilization device, a convex-concave part is arranged on a nitrocellulose membrane DNA chip cover, and the probe is arranged on the convex part. For example, a plurality of probes may be uniformly or unevenly provided in a predetermined position area of the convex portion, and a light hole may be provided in the concave portion.
On the other hand, the invention also provides a banknote biological sterilization method, which adopts the banknote biological sterilization device to sterilize banknotes, and comprises the following steps:
initializing: the working mode of the device is determined. Such as voltage output of a photoelectric driving device, fixation of ultraviolet lamps and the like, preparation for putting money and the like. Placing paper money (for example, 100 paper money) into a paper money operation box through a paper money inlet and outlet, starting the paper money operation box and a photoelectric driving device, forming photocurrent and ultraviolet rays in lamp tubes of a first sterilizing lamp and a second sterilizing lamp, irradiating the photocurrent and the ultraviolet rays onto a die tetrahedral convex lens through a light lamp hole, and reflecting and activating bacterial clusters on the paper money; simultaneously heating the inner side walls of the first nitrocellulose membrane DNA chip cover and the second nitrocellulose membrane DNA chip cover, pushing probes on the outer side walls of the first nitrocellulose membrane DNA chip cover and the second nitrocellulose membrane DNA chip cover to release a cluster burst factor which induces and kills rotation, and enabling bacterial clusters to start to rotate in opposite directions; the photoelectric driving device drives the first sterilizing lamp and the second sterilizing lamp to gather towards the lamp post door frame in opposite directions and separate in opposite directions to move back and forth through rolling along the slideway, and respectively drives the probes on the first nitrocellulose membrane DNA chip cover and the second nitrocellulose membrane DNA chip cover to operate (the operation speed is synchronous with the single banknote discharging speed of the banknote operation box, the catching and killing operation is carried out for 14 times per second, one banknote discharging and killing operation is synchronous with the speed of the twisting banknote wheel, the banknote operation box banknote twisting part moves synchronously with the upper driving wheel and the lower driving wheel left and right, the front and back sides of the banknote are caught and killed, the virus bacteria are extruded in opposite directions and left and right in a concave-convex manner (the secondary catching and killing of the bacteria is realized), the virus bacteria are rapidly gathered in the lamp post door frame area in the middle of the slideway, and the bacteria are killed under the combined action of heat reflected by the convex lens, ultraviolet irradiation and the phage burst factors released by the probes; after the banknote is put once, the banknote is put next time and circularly carried out; the accumulated killed bacteria were collected by a bacteria funnel.
The paper money biological sterilization device and the method are combined by an optical principle and a molecular imprinting technology principle to manufacture the device. Firstly, an optical theory is applied to fuse an LED lamp wick and ultraviolet light, ultraviolet light is placed in an LED lamp tube to store light energy, a light column lamp is manufactured, and bacterial biomolecules on paper money are activated to promote bacterial agglomeration through a light irradiation function. Secondly, bacterial cells are extracted by using a molecular imprinting technology theory to prepare a DNA chip, the DNA chip is transcribed on a nitrocellulose membrane chip membrane to prepare a nitrocellulose membrane DNA chip cover, and a probe on the DNA chip cover releases a phage burst factor to sterilize bacteria.
The invention has the beneficial effects that:
the invention provides a high-tech paper currency biological sterilization device, wherein the paper currency biological sterilization device realizes the full coverage of paper currency sterilization by adopting three technologies of DNA biotechnology, electric heating technology and photoelectric technology in a centralized way, and has the following advantages:
1. the sterilizing performance of the paper currency is improved, the paper currency is environment-friendly, and the physical health of people is protected.
2. Can reduce the back cage and destroy of incomplete and stained paper money in the circulation process.
3. The method is beneficial to the financial institutions to maintain the authority of the paper currency by mainly sterilizing the bacteria of the paper currency.
Drawings
The drawings described herein are for illustration purposes only and are not intended to limit the scope of the present disclosure in any way. In addition, the shapes, proportional sizes, and the like of the respective components in the drawings are merely illustrative for aiding in understanding the present invention, and are not particularly limited. Those skilled in the art with access to the teachings of the present invention can select a variety of possible shapes and scale sizes to practice the present invention as the case may be.
FIG. 1 is a top view of a banknote biological sterilization apparatus according to the present invention;
FIG. 2 is a side view of the banknote biological sterilization apparatus of the present invention;
FIG. 3 is a schematic representation of the unordered arrangement of banknote bacteria into clusters according to the present invention;
FIG. 4 is a schematic diagram showing rapid accumulation of viral bacteria in the frame region of a lamppost and release of a phage burst factor in accordance with the present invention;
FIG. 5 is a schematic diagram of a nitrocellulose membrane DNA chip cover of a banknote biological sterilization device according to the present invention;
FIG. 6 is a schematic diagram showing the rotation of bacteria clusters on a reflection excited banknote by the light of the banknote biological sterilization device of the present invention irradiated onto the four-sided convex lens of the mold through the light lamp hole;
FIG. 7 is a schematic diagram showing the development of a DNA biochip on a nitrocellulose membrane DNA chip cover according to the present invention;
FIG. 8 is a schematic development of a DNA biochip on a nitrocellulose membrane DNA chip cover according to the present invention;
description of the drawings:
the die comprises a die body 1, a convex lens 2, a first sterilizing lamp 3, a second sterilizing lamp 4, a first nitrocellulose membrane DNA chip cover 5, a second nitrocellulose membrane DNA chip cover 6, a slide way 7, a lamp post door frame 8, a bacterial funnel 9, a paper money inlet and outlet 10 and a paper money operation box 11.
Detailed Description
The technical solution of the present invention will be described in detail below for a clearer understanding of technical features, objects and advantageous effects of the present invention, but should not be construed as limiting the scope of the present invention.
The embodiment provides a paper currency biological sterilization device. As shown in fig. 1 and 2, the biological sterilization device includes: the die comprises a die body 1, a convex lens 2, a first sterilizing lamp 3, a second sterilizing lamp 4, a first nitrocellulose membrane DNA chip cover 5 and a second nitrocellulose membrane DNA chip cover 6;
the die body 1 is of a rectangular box structure, and four inner side walls of the box body are respectively provided with one convex lens 2 (the radian of the convex surface is 15 degrees, but the die is not limited to the convex lens); two symmetrical horizontal slide ways 7 are fixedly arranged on the two symmetrical convex lenses, and the slide ways 7 are parallel to the inner side walls of the box bodies corresponding to the two symmetrical convex lenses; the first sterilizing lamp 3 and the second sterilizing lamp 4 are long tubular, the length direction is perpendicular to the slide ways 7 respectively, two ends of the first sterilizing lamp 3 and the second sterilizing lamp 4 are movably connected with the two horizontal slide ways 7 respectively, and the slide ways 7, the first sterilizing lamp 3 and the second sterilizing lamp 4 are electrically connected with a photoelectric driving device respectively; when the banknote biological sterilization device operates, the photoelectric driving device is started to drive the first sterilization lamp 3 and the second sterilization lamp 4 to roll towards the middle part of the slideway 7 along the two ends of the slideway 7 to gather together oppositely and separate from each other;
a straight-strip-shaped lamp post door frame 8 is arranged in the middle of a slideway 7 in which the first sterilizing lamp 3 and the second sterilizing lamp 4 are gathered in opposite directions, the lamp post door frame 8 is perpendicular to the slideway 7 and parallel to the first sterilizing lamp 3 and the second sterilizing lamp 4, a gap which is perpendicular to the slideway 7 and parallel to the first sterilizing lamp 3 and the second sterilizing lamp 4 is arranged in the middle of the lamp post door frame 8, and the lower end of the gap is communicated with a bacterial hopper 9; when the banknote biological sterilization device operates, the first sterilization lamp 3 and the second sterilization lamp 4 gather virus bacteria to the lamp post door frame 8 for sterilization, and the virus bacteria are sent to the bacteria hopper 9 for collection through the gap of the lamp post door frame 8;
the lampwicks of the first sterilizing lamp 3 and the second sterilizing lamp 4 are LED lampwicks, ultraviolet light (the wavelength of the ultraviolet light is 250-260 nm) is filled in the lamp tube, and two ends of the lamp tube are sealed by a porcelain lamp cap and are electrically connected with a photoelectric driving device.
The first nitrocellulose membrane DNA chip cover 5 and the second nitrocellulose membrane DNA chip cover 6 are respectively sleeved outside the first sterilizing lamp 3 and the second sterilizing lamp 4. The first nitrocellulose membrane DNA chip cover 5 and the second nitrocellulose membrane DNA chip cover 6 are fixedly sleeved outside the first sterilizing lamp 3 and the second sterilizing lamp 4 through bolts, and the annular gap distance is 0.2cm.
The first nitrocellulose membrane DNA chip cover 5 or the second nitrocellulose membrane DNA chip cover 6 is prepared by:
extracting purified DNA from bursting bacteria (tubercle bacillus, hepatitis B virus and the like, but not limited to the bacteria) by utilizing a nano magnetic bead extraction method, preparing a rotary probe according to a bacteria rotary agglomeration rule, fixing the rotary probe on a nitrocellulose membrane through transcription, and rotating the obtained product into a cylinder shape to prepare the first nitrocellulose membrane DNA chip cover 5 or the second nitrocellulose membrane DNA chip cover 6, wherein the probe is positioned on the outer side wall of the first nitrocellulose membrane DNA chip cover 5 or the second nitrocellulose membrane DNA chip cover 6. The outer side wall of the first nitrocellulose membrane DNA chip cover 5 or the second nitrocellulose membrane DNA chip cover 6 is of a rugged structure, the convex part is provided with a probe, and the concave part is provided with a light lamp hole (illumination hole) (as shown in figure 5); the number of probes is 120 (but not limited thereto). The gene microarray formed by the probe arrangement is a DNA biological gene chip (shown in FIG. 7 and FIG. 8).
The side wall of the die body 1 is provided with a bill inlet and outlet 10, and the bill inlet and outlet 10 is opened upwards at an inclined plane of 30 degrees (without limitation) with the lower end surface of the die body 1. The lower end opening of the die body 1 is communicated with and connected with a paper money operation box 11 with a paper money twisting function; the banknote passes through the banknote inlet and outlet 10 into the banknote handling box 11. When the banknote handling box 11 performs banknote twisting, the banknote distance between the banknote inlet and outlet 10 and the first nitrocellulose membrane DNA chip cover 5 and the second nitrocellulose membrane DNA chip cover 6 is 2cm (but not limited thereto). The side wall of the die body 1 is provided with a USB flash disk socket for electrically connecting the banknote handling box 11. The mold body 1 may be provided in a size of 17cm long, 5cm wide, and 4cm high, but is not limited thereto.
In a preferred embodiment, the banknote handling machine is configured as an ATM banknote handling machine, a banknote counter or a banknote bundling machine, as required. When the Automatic Teller Machine (ATM) preparation operation machine is arranged, the upper end of the die body 1 is electrically connected with an operation system box, so that the integration of sterilization and money taking operation is realized.
In a preferred embodiment, a method for manufacturing a banknote biological sterilization apparatus is provided, comprising:
first, a germicidal lamp (LED lamp core and ultraviolet light)
In the banknote biological sterilization device, a tube of a sterilization lamp is made of quartz glass material into a hollow tube; the lamp core in the lamp tube adopts an LED lamp core filament, fluorescent powder is arranged on the inner wall of the lamp tube, the lamp tube uses a hot cathode to discharge mercury vapor under low pressure, ultraviolet light is injected into the lamp tube, the lamp tube is sealed by a porcelain lamp cap, and the wavelength of the ultraviolet light is 250-260 nm; the diameter of the lamp tube was set to 0.5cm.
Second step, preparing nitrocellulose membrane DNA chip cover
The nitrocellulose membrane is adopted to prepare a nitrocellulose membrane DNA chip cover, and 120 balanced convex spike needle bar probes are arranged outside the chip cover; the chip cover and the sterilizing lamp are sleeved and fixed by bolts, and the distance between the chip cover and the sterilizing lamp is 0.2cm.
And (3) preparing a DNA biological gene chip. Extracting molecular cells of pop bacteria such as tubercle bacillus and hepatitis B virus bacteria. Decomposing bacterial gene protein molecules and extracting bursting bacterial molecule genes. The probe made of virus carrying burst gene protein is solidified on nitrocellulose membrane to make DNA chip, and in situ hybridization is carried out. After signal detection and result analysis, DNA is cloned and duplicated after heating and drying to prepare a DNA biological gene chip probe, and the probe is transcribed on a nitrocellulose membrane lampshade. The gene microarray formed by the probe arrangement is a DNA biological gene chip.
The specific steps for manufacturing the probe are as follows:
1. 0.5-1. Mu.L of fresh protein was extracted from the burster bacteria and DNA samples sufficient for N-fold successful amplification were extracted.
2. Firstly, preparing 5-8nm magnetic nano particles, so that the magnetic nano particles have very high magnetic field response capability and superparamagnetic property, and nano regulation and control can be realized by changing an external magnetic field;
3. coating the magnetic nano particles by using a coating technology;
4. by utilizing a surface chemical modification technology, functional groups which can specifically act on DNA are connected, and the modified functional groups control the reversible adsorption property of the DNA;
5. the accurate amount of DNA is obtained by surface modification and control of the conditions such as the ionic strength, the pH value and the like in the solution.
6. Extracting and purifying DNA, using strong protein denaturants such as guanidine thiocyanate, destroying cell membrane and nuclear membrane proteins, releasing DNA, and inactivating nuclease;
7. adding magnetic beads, and carrying out specific adsorption on DNA through chemical groups on the surface;
8. separating the magnetic particles from the liquid and recovering the particles;
9. the adsorbed DNA was eluted with pure water or TE, and the DNA was dissociated from the magnetic beads in the solution to re-elute the DNA.
10. And (3) preparing a new probe, coating the DNA magnetic nano particles for the second time, preparing a rotary probe according to a bacterial rotation agglomeration rule by using an ink-jet printer or an artificial robot mode, and transferring and fixing the rotary probe on a nitrocellulose membrane cover.
Third, manufacturing a convex lens reflecting device
The periphery of the inner wall of the die is provided with reflective convex lenses, the four-sided convex lenses of the die and the inner wall of the die form an oblique angle (convex radian) of 15 degrees, and bacteria are accelerated to form clusters through photocurrent pairs in a photoelectric driving device. Meanwhile, the inner side wall of the nitrocellulose membrane DNA chip cover is heated to 80 ℃ through photocurrent in the photoelectric driving device, the irradiated convex lens is reflected to the nitrocellulose membrane DNA chip cover, and a plurality of convex spike needle bar probes and concave pinholes are arranged on the outer side wall of the nitrocellulose membrane DNA chip cover.
Fourth step, convex wolf tooth needle bar probe and concave needle hole
The current heating device and the lens reflecting device are used for heating, illuminating and pushing the probe to release the trapping and killing rotary cluster bursting factors to kill different bacteria on paper money.
In a preferred embodiment, there is provided a banknote sterilization method using the banknote sterilization apparatus, comprising the steps of:
initializing: the working mode of the device is determined. Such as voltage output of a photoelectric driving device, fixation of ultraviolet lamps and the like, preparation for putting money and the like. Placing paper money (for example, 100 paper money) in a paper money operation box 11 through a paper money inlet and outlet, starting the paper money operation box 11 and a photoelectric driving device, forming photocurrent and ultraviolet rays in the lamp tubes of the first sterilizing lamp 3 and the second sterilizing lamp 4, irradiating the photocurrent and the ultraviolet rays onto the die tetrahedron lens 2 through a light lamp hole, and reflecting and activating bacterial clusters on the paper money; simultaneously heating the inner side walls of the first nitrocellulose membrane DNA chip cover 5 and the second nitrocellulose membrane DNA chip cover 6, pushing the probes of the outer side walls of the first nitrocellulose membrane DNA chip cover 5 and the second nitrocellulose membrane DNA chip cover 6 to release a decoy rotation cluster burst factor, and pushing the bacterial clusters to start to rotate in opposite directions (as shown in a, b and c in FIG. 3 and FIG. 4); the photoelectric driving device drives the first sterilizing lamp 3 and the second sterilizing lamp 4 to gather towards the lamp post door frame 8 in opposite directions and separate from each other to move back and forth rapidly through rolling along the slideway 7, respectively drives probes on the first nitrocellulose membrane DNA chip cover 5 and the second nitrocellulose membrane DNA chip cover 6 to operate (the operation speed is synchronous with the single banknote discharging speed of the banknote operation box, the catching and killing operation is 14 times per second, one banknote discharging and killing operation is synchronous with the speed of the twisting banknote, the banknote twisting part of the banknote operation box 11 moves synchronously with the upper driving wheel and the lower driving wheel to catch and kill the front and the back sides of the banknote, the virus bacteria are extruded in a way of being concave and convex towards the left side and the right side (realizing secondary catching and killing of the bacteria), and the virus bacteria are gathered in the lamp post door frame 8 area in the middle of the slideway rapidly, and the bacteria are caught and killed through the combined action of the heat (as shown in fig. 6) reflected by the convex lens 2, ultraviolet irradiation and the phagocytic factor released by the probes (the diameter of the opening of the probe on the surface of the banknote virus bacteria is 0.01-0.02 mm); after the banknote is put once, the banknote is put next time and circularly carried out; the accumulated killed bacteria were collected by a bacteria funnel.
The paper money biological sterilization device and the method are combined by an optical principle and a molecular imprinting technology principle to manufacture the device. Firstly, an optical theory is applied to fuse an LED lamp wick and ultraviolet light, ultraviolet light is placed in an LED lamp tube to store light energy, a light column lamp is manufactured, and bacterial biomolecules on paper money are activated to promote bacterial agglomeration through a light irradiation function. Secondly, bacterial cells are extracted by using a molecular imprinting technology theory to prepare a DNA chip, the DNA chip is transcribed on a nitrocellulose membrane chip membrane to prepare a nitrocellulose membrane DNA chip cover, and a probe on the nitrocellulose membrane DNA chip cover releases a phage burst factor to sterilize bacteria.
The above embodiments are provided to illustrate the technical concept and features of the present invention and are intended to enable those skilled in the art to understand the content of the present invention and implement the same, and are not intended to limit the scope of the present invention. All equivalent changes or modifications made in accordance with the spirit of the present invention should be construed to be included in the scope of the present invention.
Claims (16)
1. A banknote biological sterilization device, which is characterized in that: the biological sterilization device comprises a die body, a convex lens, a first sterilization lamp, a second sterilization lamp, a first nitrocellulose membrane DNA chip cover and a second nitrocellulose membrane DNA chip cover;
the die body is of a rectangular box structure, and one convex lens is respectively distributed on the four inner side walls of the box body;
two symmetrical horizontal slide ways are fixedly arranged on the two symmetrical convex lenses, and are parallel to the inner side walls of the box bodies corresponding to the two symmetrical convex lenses; the first sterilizing lamp and the second sterilizing lamp are long-tube-shaped, the length direction of the first sterilizing lamp and the second sterilizing lamp are respectively perpendicular to the slide ways, two ends of the first sterilizing lamp and two ends of the second sterilizing lamp are respectively and movably connected with two horizontal slide ways, and the slide ways, the first sterilizing lamp and the second sterilizing lamp are respectively and electrically connected with a photoelectric driving device; when the paper money biological sterilization device operates, the photoelectric driving device is started to drive the first sterilization lamp and the second sterilization lamp to roll towards the middle part of the slideway along the two ends of the slideway to gather together oppositely and separate oppositely;
a straight-strip-shaped lamp post door frame is arranged in the middle of a slideway where the first sterilizing lamp and the second sterilizing lamp are gathered in opposite directions, the lamp post door frame is perpendicular to the slideway and parallel to the first sterilizing lamp and the second sterilizing lamp, a gap perpendicular to the slideway and parallel to the first sterilizing lamp and the second sterilizing lamp is arranged in the middle of the lamp post door frame, and the lower end of the gap is communicated with a bacterial hopper; when the paper money biological sterilization device operates, the first sterilization lamp and the second sterilization lamp gather virus bacteria to the lamp post door frame for sterilization, and the virus bacteria are sent to the bacteria hopper for collection through a gap of the lamp post door frame;
the first nitrocellulose membrane DNA chip cover and the second nitrocellulose membrane DNA chip cover are respectively sleeved on the outer sides of the first sterilizing lamp and the second sterilizing lamp;
the preparation method of the first nitrocellulose membrane DNA chip cover or the second nitrocellulose membrane DNA chip cover comprises the following steps:
extracting purified DNA from bursting bacteria by using a nano magnetic bead extraction method, preparing a rotary probe according to a bacteria rotation agglomeration rule, fixing the rotary probe on a nitrocellulose membrane by transcription, and rotating the obtained product into a cylinder shape to prepare a first nitrocellulose membrane DNA chip cover or a second nitrocellulose membrane DNA chip cover, wherein the probe is positioned on the outer side wall of the first nitrocellulose membrane DNA chip cover or the second nitrocellulose membrane DNA chip cover.
2. The banknote biological sterilization apparatus of claim 1, wherein: the lamp cores of the first sterilizing lamp and the second sterilizing lamp are LED lamp cores, ultraviolet light is filled in the lamp tube, and two ends of the lamp tube are sealed through the porcelain lamp cap and are electrically connected with the photoelectric driving device.
3. The banknote biological sterilization apparatus of claim 2, wherein: the wavelength of the ultraviolet light is 250-260nm.
4. The banknote biological sterilization apparatus of claim 1, wherein: the first nitrocellulose membrane DNA chip cover and the second nitrocellulose membrane DNA chip cover are fixedly sleeved on the outer sides of the first sterilizing lamp and the second sterilizing lamp through bolts, and the annular gap distance is 0.2cm.
5. The banknote biological sterilization apparatus of claim 1, wherein: the outer side walls of the first nitrocellulose membrane DNA chip cover and/or the second nitrocellulose membrane DNA chip cover are of uneven structures, the protruding parts are provided with probes, and the recessed parts are provided with light lamp holes.
6. The banknote biological sterilization apparatus of claim 1, wherein: the number of probes on the first nitrocellulose membrane DNA chip cover or the second nitrocellulose membrane DNA chip cover is 120.
7. The banknote biological sterilization apparatus of claim 1, wherein: the bursting bacteria comprise tuberculosis bacteria and/or hepatitis B virus bacteria.
8. The banknote biological sterilization apparatus of claim 1, wherein: the side wall of the die body is provided with a paper money inlet and a paper money outlet, and the paper money inlet and the paper money outlet are opened upwards at an inclined plane of 30 degrees with the lower end surface of the die body.
9. The banknote biological sterilization apparatus of claim 8, wherein: the lower end opening of the die body is communicated with a banknote handling box with banknote twisting function; the banknote enters the banknote handling box through the banknote inlet and outlet.
10. The banknote biological sterilization apparatus of claim 9, wherein: the paper money operation box is set as an ATM paper money operation machine, a paper money counter or a paper money bundling machine according to the requirement.
11. The banknote biological sterilization apparatus of claim 9, wherein: when the banknote handling box twists banknote, the distance between the banknote at the banknote inlet and outlet and the plane of the first nitrocellulose membrane DNA chip cover and the second nitrocellulose membrane DNA chip cover is 2cm.
12. The banknote biological sterilization apparatus of claim 9, wherein: the side wall of the die body is provided with a USB flash disk socket for electrically connecting the paper currency operation box.
13. The banknote biological sterilization apparatus of claim 1, wherein: the upper end of the die body is electrically connected with an operating system box.
14. The banknote biological sterilization apparatus of claim 1, wherein: the length of the die body is 17cm, the width of the die body is 5cm, and the height of the die body is 4cm.
15. The banknote biological sterilization apparatus of claim 1, wherein: the convex radian of the convex lens is 15 degrees.
16. A banknote sterilization method using the banknote sterilization apparatus according to any one of claims 1 to 15, comprising the steps of:
the paper money is placed in the paper money operation box through the paper money inlet and outlet, the paper money operation box and the photoelectric driving device are started, photocurrent and ultraviolet rays are formed in the lamp tubes of the first sterilizing lamp and the second sterilizing lamp, and are irradiated onto the four-sided convex lens of the die through the light lamp holes to reflect and activate bacterial clusters on the paper money; simultaneously heating the inner side walls of the first nitrocellulose membrane DNA chip cover and the second nitrocellulose membrane DNA chip cover, pushing probes on the outer side walls of the first nitrocellulose membrane DNA chip cover and the second nitrocellulose membrane DNA chip cover to release a cluster burst factor which induces and kills rotation, and enabling bacterial clusters to start to rotate in opposite directions; the photoelectric driving device drives the first sterilizing lamp and the second sterilizing lamp to gather towards the lamp post door frame oppositely and separate from each other to move back and forth rapidly through rolling along the slideway, and respectively drives the probes on the first nitrocellulose membrane DNA chip cover and the second nitrocellulose membrane DNA chip cover to run, so that virus bacteria are extruded to the left and right in a concave-convex manner, the virus bacteria are gathered to the lamp post door frame area in the middle of the slideway rapidly, and the bacteria are killed under the combined action of heat reflected by the convex lens, ultraviolet irradiation and phage burst factors released by the probes; the accumulated killed bacteria were collected by a bacteria funnel.
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| CN2038006U (en) * | 1988-08-04 | 1989-05-24 | 浙江省新安江水泥制品厂 | Sterilization device for paper money |
| CN107648631A (en) * | 2017-10-16 | 2018-02-02 | 中科镭盾（北京）科技有限公司 | Pulsed xenon lamp ultraviolet sterilization instrument |
| CN208426446U (en) * | 2018-03-06 | 2019-01-25 | 中国工商银行股份有限公司 | A kind of bank note biological antibiotic device |
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| US20130045133A1 (en) * | 2011-08-20 | 2013-02-21 | Vialux Systems, Inc. | Methods and apparatus for commerce media sterilization |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2038006U (en) * | 1988-08-04 | 1989-05-24 | 浙江省新安江水泥制品厂 | Sterilization device for paper money |
| CN107648631A (en) * | 2017-10-16 | 2018-02-02 | 中科镭盾（北京）科技有限公司 | Pulsed xenon lamp ultraviolet sterilization instrument |
| CN208426446U (en) * | 2018-03-06 | 2019-01-25 | 中国工商银行股份有限公司 | A kind of bank note biological antibiotic device |
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