CN108159258A - Treatment is with Chinese medicine for treating diabetes composition of lumbar vertebra disease and preparation method thereof - Google Patents
Treatment is with Chinese medicine for treating diabetes composition of lumbar vertebra disease and preparation method thereof Download PDFInfo
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- A61K36/88—Liliopsida (monocotyledons)
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Abstract
The present invention provides a kind for the treatment of with Chinese medicine for treating diabetes composition of lumbar vertebra disease and preparation method thereof, which is made of the raw material of following weight parts：10~20 parts of 120~130 parts of lycopodium calvatum, 110~125 parts of rhizoma atractylodis, 100~110 parts of Radix Glycyrrhizae, 80~95 parts of Radix Ophiopogonis, 75~90 parts of Schisandra chinensis, 60~75 parts of Rhizoma Chuanxiong, 40~55 parts of pueraria lobata, 40~50 parts of Radix Paeoniae Alba, 35~45 parts of Poria cocos, 30~40 parts of rhizoma alismatis, 20~30 parts of Chinese cassia tree, 10~20 parts of Chinese yam and ginsenoside.The Chinese medicine composition of the present invention can effectively control blood glucose, treat the diabetes with lumbar vertebra disease intercurrent disease, patient is made to get well, with obvious effects, treatment cycle is short and recurrence rate is low, will not generate the dependence to drug.
Description
Technical field
The invention belongs to diabetes medicament fields, and in particular to a kind of Chinese medicine for treating diabetes composition treated with lumbar vertebra disease
And preparation method thereof.
Background technology
Diabetes be a kind of complex disease because syndrome, be the hormone due to internal insulin deficit or antagonism insulin
Increase or cannot bring into normal play in target cell glucose caused by physiological action, protein and lipid-metabolism of insulin is disorderly
A kind of random syndrome.It is characterized in that occur when concentration of glucose raising and glucose in urine, blood glucose extremely are excessively high in blood circulation typical
" three-many-one-little " symptom, i.e., more drinks, diuresis are eaten and weight loss, and with fatigue and weak more.Ketosis acid can occur for severe patient
Poisoning, hypertonicity diabetic coma, and easily merge multi-infection.With the extension of the course of disease, metabolic disorder can cause eye, kidney,
The chronic disease of the histoorgans such as nerve, blood vessel and heart.If heart disease, brain occur for treatment that cannot be timely, appropriate
Vascular lesion, renal failure, lose the sight of both eyes, lower limbs necrosis situations such as, become the main reason for disabling, is lethal.And diabetes
Incidence is high, and either developed country or developing country, the incidence of diabetes are all rising year by year.
Some patientss take drug or the insulin injection of a large amount of hypoglycemic to treat diabetes, the state of an illness as a result occur
Tolerance, the antidiabetic drug for only taking a large amount of dosage can just have hypoglycemic effect, and can also be generated to medicine using insulin
The dependence of object, causes blood glucose to be difficult to control.The main reason for such case is caused to occur, is is that people " control diabetes in time
Treat " misunderstanding, the traditional Chinese medical science is more correct to the cause of disease view of diabetes, it is believed that mainly overfeeding delicious food, five excessive emotional activities, sexual overstrain,
The insufficient several factors of the pyreticosis dry and native endowment of fire.In fact for the treatment of diabetes, patient answers reasonable meals in daily life
Food, controls and forms good eating habit, is then treated by appropriate Chinese medicine, could effectively control blood glucose, stablizes disease
Feelings are got well.However diabetes, also often with hyperglycemia, the intercurrent diseases such as hyperlipidemia and lumbar vertebra disease, this is controlled to Chinese medicine
Treating increases its difficulty, therefore researches and develops to have to treat and be had a very important significance with the Chinese medicine for treating diabetes of lumbar vertebra disease.
Invention content
The object of the present invention is to provide a kind of effective treatment with the Chinese medicine for treating diabetes composition of lumbar vertebra disease.
In order to achieve the above objectives, Chinese medicine composition of the present invention is made of the raw material of following weight parts：Lycopodium calvatum 120~
130 parts, 110~125 parts of rhizoma atractylodis, 100~110 parts of Radix Glycyrrhizae, 80~95 parts of Radix Ophiopogonis, 75~90 parts of Schisandra chinensis, 60~75 parts of Rhizoma Chuanxiong,
40~55 parts of pueraria lobata, 40~50 parts of Radix Paeoniae Alba, 35~45 parts of Poria cocos, 30~40 parts of rhizoma alismatis, 20~30 parts of Chinese cassia tree, 10~20 parts of Chinese yam
With 10~20 parts of ginsenoside.
In the present invention, the extracting mode step of the Rhizoma Chuanxiong active ingredient is as follows：
A) Rhizoma Chuanxiong is taken to shred, 60% ethanol wet 8~15 minutes；
B) 65% ethyl alcohol is added in into the medicinal material after wetting to impregnate 6~10 hours；
C) medicinal material after immersion is placed in percolate pot to 75% alcohol solvent for adding in 5~6 times of medicinal material amounts, with 160~
The speed of 180ml/min/kg medicinal materials carries out diacolation；
D) percolate is collected, removal solvent is up to Rhizoma Chuanxiong extract.
In the present invention, the extracting mode step of the effective component of paeonia lactiflora is as follows：
A) Radix Paeoniae Alba is taken to shred, 55% ethanol wet 10~15 minutes；
B) 70% ethyl alcohol is added in into the medicinal material after wetting to impregnate 10~15 hours；
C) medicinal material after immersion is placed in percolate pot to 75% alcohol solvent for adding in 5~6 times of medicinal material amounts, with 160~
The speed of 180ml/min/kg medicinal materials carries out diacolation；
D) percolate is collected, removal solvent is up to radix paeoniae alba extraction.
In the present invention, 50~60% ethanol percolations of the Schisandra chinensis collect percolate, recycle ethyl alcohol, are concentrated into thick paste.
Preferably, Chinese medicine composition of the present invention is made of the raw material of following weight parts：125 parts of lycopodium calvatum, rhizoma atractylodis 115
Part, 105 parts of Radix Glycyrrhizae, 90 parts of Radix Ophiopogonis, 80 parts of Schisandra chinensis, 70 parts of Rhizoma Chuanxiong, 45 parts of pueraria lobata, 45 parts of Radix Paeoniae Alba, 40 parts of Poria cocos, rhizoma alismatis 35
15 parts of part, 25 parts of Chinese cassia tree, 15 parts of Chinese yam and ginsenoside.
Another object of the present invention provides the preparation method of the Chinese medicine composition, which is：
(1) medicinal material of parts by weight is spare needed for weighing；
(2) Rhizoma Chuanxiong is taken to shred, 60% ethanol wet 8~15 minutes adds in 65% ethyl alcohol into the medicinal material after wetting and impregnates 6
~10 hours, the medicinal material after immersion is placed in percolate pot to 75% alcohol solvent for adding in 5~6 times of medicinal material amounts, with 160~
The speed of 180ml/min/kg medicinal materials carries out diacolation, collects percolate, removal solvent is up to Rhizoma Chuanxiong extract；
(3) Radix Paeoniae Alba is taken to shred, 55% ethanol wet 10~15 minutes adds in the immersion of 70% ethyl alcohol into the medicinal material after wetting
10~15 hours, the medicinal material after immersion is placed in percolate pot to 75% alcohol solvent for adding in 5~6 times of medicinal material amounts, with 160~
The speed of 180ml/min/kg medicinal materials carries out diacolation, collects percolate, removal solvent is up to radix paeoniae alba extraction；
(4) 35~45 degrees Celsius of flooding 2 times of Radix Ophiopogonis, 1~1.5 hour every time, merges leaching liquor, filtration, filtrate
It is concentrated into the thick paste that relative density is 1.2~1.3；
(5) 50~60% ethanol percolations of Schisandra chinensis collect percolate, recycle ethyl alcohol, are concentrated into thick paste；
(6) after Chinese cassia tree is crushed, with the extraction of 70%~85% ethanol solution twice, merge extracting solution, extracting solution is concentrated
To no alcohol taste, the water of doubling dose is added to dilute and is stirred evenly, stands and removes sediment, obtain cinnamomum cassia extract；
(7) lycopodium calvatum, rhizoma atractylodis, Radix Glycyrrhizae, pueraria lobata, Poria cocos, rhizoma alismatis water decoct 3~4 times, 2.5~3.5 hours every time, close
And decoction liquor, filtering, filtrate are concentrated into the thick paste that relative density is 1.15~1.25, cooling, adding in ethyl alcohol makes the alcohol content be
40%~60%, stand, take supernatant, recycle ethyl alcohol, concentration and gained thick paste in Chinese yam fine powder, ginsenoside, (2)-(6) and
Extract merges, and dry, adding pharmaceutic adjuvant, routinely dosage form needed for clinic is made in preparation process.
Chinese medicine composition of the present invention, can be prepared by conventional preparation process becomes clinically-acceptable various dosage forms
Such as：Granule, tablet, pill, capsule, injection or oral liquid, tincture, suppository, mixture, powder, lotion, film, dripping pill
Deng.
In the present invention, ethylhydroxyethylcellulose, chitin, methyl can also be added in preparation made from Chinese medicine composition
Cellulose, sodium alginate, potassium alginate, calcium alginate, hydroxyethyl cellulose, hydroxypropyl cellulose, dextrin etc..
Preferably, the dosage form of Chinese medicine composition of the present invention is granule, every gram of the granule containing ginsenoside Re 3~
6mg。
In the present invention, the assay method of the ginsenoside Re can be：
The preparation of reference substance solution：It is appropriate that precision weighs ginsenoside Re's reference substance, and methanol is added to be respectively prepared in every 1ml and is contained
The solution of ginsenoside Re 0.4mg, as reference substance solution；
The preparation of test solution：This product is taken, is ground well, precision weighs 1 gram, and add methylene chloride 15ml, and water-bath reflux 0.5 is small
When, filtration discards dichloromethane, and residue volatilizes adds the water 2ml of n-butanol saturation soak after solvent together with filter paper, and precision adds in use
Water-saturated n-butanol 40ml ultrasonic extraction 30min, precision measure supernatant, and twice times of amount of ammonification test solution shakes up, take upper strata molten
Liquid is evaporated, and residue adds ethyl alcohol to dissolve, and quantitatively moves in 2ml measuring bottles, ethyl alcohol is added to be diluted to scale, shake up to get；
Chromatographic condition：It is filler with octadecylsilane chemically bonded silica；
- 0.05% phosphoric acid solution of acetonitrile that volume ratio is 50: 80 is mobile phase；Detection wavelength 203nm；Measuring method：Respectively
Precision draws reference substance solution and each 10 μ l of test solution, injects hplc determination.
The Chinese medicine composition of the present invention can effectively control blood glucose, treat the diabetes with lumbar vertebra disease intercurrent disease, make disease
People gets well, with obvious effects, and treatment cycle is short and recurrence rate is low, will not generate the dependence to drug, blood glucose is caused to be difficult to
Control.
Specific embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.As long as in addition, technical characteristic involved in the various embodiments of the present invention described below each other it
Between do not form conflict and can be combined with each other.
Embodiment 1：
The present embodiment Chinese medicine composition is made of the raw material of following weight parts：It is 125 parts of lycopodium calvatum, 115 parts of rhizoma atractylodis, sweet
105 parts of grass, 90 parts of Radix Ophiopogonis, 80 parts of Schisandra chinensis, 70 parts of Rhizoma Chuanxiong, 45 parts of pueraria lobata, 45 parts of Radix Paeoniae Alba, 40 parts of Poria cocos, 35 parts of rhizoma alismatis, Chinese cassia tree
15 parts of 25 parts, 15 parts of Chinese yam and ginsenoside.
The Chinese medicine composition of the present embodiment is prepared by following preparation methods：
(1) medicinal material of parts by weight is spare needed for weighing；
(2) Rhizoma Chuanxiong is taken to shred, 60% ethanol wet 8~15 minutes adds in 65% ethyl alcohol into the medicinal material after wetting and impregnates 6
~10 hours, the medicinal material after immersion is placed in percolate pot to 75% alcohol solvent for adding in 5~6 times of medicinal material amounts, with 160~
The speed of 180ml/min/kg medicinal materials carries out diacolation, collects percolate, removal solvent is up to Rhizoma Chuanxiong extract；
(3) Radix Paeoniae Alba is taken to shred, 55% ethanol wet 10~15 minutes adds in the immersion of 70% ethyl alcohol into the medicinal material after wetting
10~15 hours, the medicinal material after immersion is placed in percolate pot to 75% alcohol solvent for adding in 5~6 times of medicinal material amounts, with 160~
The speed of 180ml/min/kg medicinal materials carries out diacolation, collects percolate, removal solvent is up to radix paeoniae alba extraction；
(4) 35~45 degrees Celsius of flooding 2 times of Radix Ophiopogonis, 1~1.5 hour every time, merges leaching liquor, filtration, filtrate
It is concentrated into the thick paste that relative density is 1.2~1.3；
(5) 50~60% ethanol percolations of Schisandra chinensis collect percolate, recycle ethyl alcohol, are concentrated into thick paste；
(6) after Chinese cassia tree is crushed, with the extraction of 70%~85% ethanol solution twice, merge extracting solution, extracting solution is concentrated
To no alcohol taste, the water of doubling dose is added to dilute and is stirred evenly, stands and removes sediment, obtain cinnamomum cassia extract；
(7) lycopodium calvatum, rhizoma atractylodis, Radix Glycyrrhizae, pueraria lobata, Poria cocos, rhizoma alismatis water decoct 3~4 times, 2.5~3.5 hours every time, close
And decoction liquor, filtering, filtrate are concentrated into the thick paste that relative density is 1.15~1.25, cooling, adding in ethyl alcohol makes the alcohol content be
40%~60%, stand, take supernatant, recycle ethyl alcohol, concentration and gained thick paste in Chinese yam fine powder, ginsenoside, (2)-(6) and
Extract merges, dry, and adding pharmaceutic adjuvant, routinely granule is made in preparation process, and every gram of particle contains ginsenoside Re
4.5mg。
Wherein, the assay method of the ginsenoside Re is：
The preparation of reference substance solution：It is appropriate that precision weighs ginsenoside Re's reference substance, and methanol is added to be respectively prepared in every 1ml and is contained
The solution of ginsenoside Re 0.4mg, as reference substance solution；
The preparation of test solution：This product is taken, is ground well, precision weighs 1 gram, and add methylene chloride 15ml, and water-bath reflux 0.5 is small
When, filtration discards dichloromethane, and residue volatilizes adds the water 2ml of n-butanol saturation soak after solvent together with filter paper, and precision adds in use
Water-saturated n-butanol 40ml ultrasonic extraction 30min, precision measure supernatant, and twice times of amount of ammonification test solution shakes up, take upper strata molten
Liquid is evaporated, and residue adds ethyl alcohol to dissolve, and quantitatively moves in 2ml measuring bottles, ethyl alcohol is added to be diluted to scale, shake up to get；
Chromatographic condition：It is filler with octadecylsilane chemically bonded silica；
- 0.05% phosphoric acid solution of acetonitrile that volume ratio is 50: 80 is mobile phase；Detection wavelength 203nm；Measuring method：Respectively
Precision draws reference substance solution and each 10 μ l of test solution, injects hplc determination.
Embodiment 2：
The present invention is to causing the influence of adult rat adrenal tissue element hyperglycemia model.
Type-2 diabetes mellitus is common complaint among the elderly, frequently-occurring disease.Through clinical efficacy and it is experimentally confirmed that Chinese traditional medicine composition of the present invention
Object has hypoglycemic effect.This experiment is usually to accelerate glycogen and lipolysis in rat body with adrenal gland, makes Portugal in rat serum
Grape sugar concentration increases, and adrenaline can also inhibit the insulin releasing in rat body.Simultaneously doses grape is filled to rat again
Sugar accelerates to cause hyperglycemia model.
Material is as follows：
Animal：Wistar rats, half male and half female, weight are 220 ± 25g
Drug：1. original powder of the present invention, effective dose is respectively 20 times of clinical application amount.
2. GUBEN JIANGTANG JIAONANG.
Method and result：
Rat 30 is taken, is randomly divided into 3 groups, every group 10.First group is negative control group, gives distilled water.Second group
For positive controls, to GUBEN JIANGTANG JIAONANG, third group gives the Chinese medicine of the present invention, and gastric infusion, medicine journey one month, last is given
1 hour after medicine, by the dosage Injection of Adrenaline of 500 μ g/kg, cause adrenal gland hyperglycemia model, at the same with glucose 1g/kg
Dosage gavage, establish to acceleration model, measure empty stomach, half an hour, 1 hour, 2 hours, 3 respectively using glucose oxidase method
The change of blood sugar of hour, the result is shown in tables 1.
Influence of 1 Chinese medicine of the present invention of table to adrenaline moulding rat blood sugar content
As a result it and discusses：
Animal hyperglycemia model caused by adrenaline is a kind of method increased with simulation irritability blood glucose, is equivalent to adult
Morbidity state after the Psychomotor test Battrey sexual stimulus of type 2 diabetes patient, therefore with certain clinical meaning.And consolidate hypoglycemic
It has been that past research institute confirms, therefore be selected to this experiment as Chinese medicine that capsule, which can be substantially reduced adrenal gland disposition hyperglycemia,
Positive control.Originally the experimental results showed that, the present invention can preferably inhibit the rat blood sugar caused by adrenaline to increase reaction,
There is apparent hypoglycemic effect, can be used for treating diabetes.
Specific case 1：
There is polydipsia in patient Mr. Zhang 47 years old before 3 years, fatigue and weak, frequent micturition urine is more, and body is gradually thin, fasting blood-glucose
10.5mmol/l, and with the symptom of lumbar vertebra disease, per hypoglycemic agent is taken before the meal, through multiple hospitals, a variety of method treatments are imitated
Fruit unobvious.Chinese medicine of the present invention is taken after 1 month, diversion is reduced, and urine is reduced, and fasting blood-glucose is maintained at 6mmol/l or so.
Specific case 2：
48 Mr. Sun due to fighting for a long time in market, has formed very bad eating habit and has been accustomed to work and rest, with sugar
Urine disease, blood glucose is unstable always, and with the symptom of lumbar vertebra disease, in order to solve this problem, grandson first ate it raw antidiabetic drug, beat
Insulin is crossed, but blood glucose still can drop, however Mr.'s Sun rational diet and work and rest, taking prescription the latter of the present invention
Month, the state of an illness is clearly better.
Specific case 3：
57 Miss Wu, with diabetes for many years, blood glucose is unstable always, and with the symptom of lumbar vertebra disease, is
Solve the problems, such as this, Miss Wu ate antidiabetic drug, beat insulin, but blood glucose still can drop, however Miss Wu closes
Diet and work and rest are managed, after persistently having taken Chinese medicine of the present invention, the state of an illness has obtained no small change, per blood glucose will after a period of time
There is steady and regular decline, persistently take two after half a month, glucostasis, fasting blood-glucose is maintained at 5.7mmol/l or so.
As it will be easily appreciated by one skilled in the art that the foregoing is merely illustrative of the preferred embodiments of the present invention, not to
The limitation present invention, all any modification, equivalent and improvement made all within the spirits and principles of the present invention etc., should all include
Within protection scope of the present invention.
Claims (10)
1. a kind of Chinese medicine for treating diabetes composition treated with lumbar vertebra disease, which is characterized in that the Chinese medicine composition includes following heavy
Measure the component of number：120~130 parts of lycopodium calvatum, 110~125 parts of rhizoma atractylodis, 100~110 parts of Radix Glycyrrhizae, 80~95 parts of Radix Ophiopogonis, the five tastes
Son 75~90 parts, 60~75 parts of Rhizoma Chuanxiong, 40~55 parts of pueraria lobata, 40~50 parts of Radix Paeoniae Alba, 35~45 parts of Poria cocos, 30~40 parts of rhizoma alismatis,
10~20 parts of 20~30 parts of Chinese cassia tree, 10~20 parts of Chinese yam and ginsenoside.
2. treatment according to claim 1 is with the Chinese medicine for treating diabetes composition of lumbar vertebra disease, which is characterized in that the Rhizoma Chuanxiong
The extracting mode step of active ingredient is as follows：
A) Rhizoma Chuanxiong is taken to shred, with 60% ethanol wet 8~15 minutes；
B) 65% ethyl alcohol is added in into the medicinal material after wetting to impregnate 6~10 hours；
C) medicinal material after immersion is placed in percolate pot to 75% alcohol solvent for adding in 5~6 times of medicinal material amounts, with 160~180ml/
The speed of min/kg medicinal materials carries out diacolation；
D) percolate is collected, removal solvent is up to Rhizoma Chuanxiong extract.
3. treatment according to claim 1 is with the Chinese medicine for treating diabetes composition of lumbar vertebra disease, which is characterized in that the Radix Paeoniae Alba
The extracting mode step of active ingredient is as follows：
A) Radix Paeoniae Alba is taken to shred, 55% ethanol wet 10~15 minutes；
B) 70% ethyl alcohol is added in into the medicinal material after wetting to impregnate 10~15 hours；
C) medicinal material after immersion is placed in percolate pot to 75% alcohol solvent for adding in 5~6 times of medicinal material amounts, with 160~180ml/
The speed of min/kg medicinal materials carries out diacolation；
D) percolate is collected, removal solvent is up to radix paeoniae alba extraction.
4. treatment according to claim 1 is with the Chinese medicine for treating diabetes composition of lumbar vertebra disease, which is characterized in that the five tastes
Sub 50~60% ethanol percolations, collect percolate, recycle ethyl alcohol, are concentrated into thick paste.
5. treatment according to claim 1 is with the Chinese medicine for treating diabetes composition of lumbar vertebra disease, which is characterized in that the Chinese medicine group
Close the component that object includes following portions by weight：125 parts of lycopodium calvatum, 115 parts of rhizoma atractylodis, 105 parts of Radix Glycyrrhizae, 90 parts of Radix Ophiopogonis, Schisandra chinensis 80
Part, 70 parts of Rhizoma Chuanxiong, 45 parts of pueraria lobata, 45 parts of Radix Paeoniae Alba, 40 parts of Poria cocos, 35 parts of rhizoma alismatis, 25 parts of Chinese cassia tree, 15 parts of Chinese yam and ginsenoside 15
Part.
6. the preparation method of the Chinese medicine for treating diabetes composition with lumbar vertebra disease is treated according to claim 1-5 any one of them,
It is characterized in that, the preparation method includes the following steps：
(1) medicinal material of parts by weight is spare needed for weighing；
(2) Rhizoma Chuanxiong is taken to shred, 60% ethanol wet 8~15 minutes adds in 65% ethyl alcohol into the medicinal material after wetting and impregnates 6~10
Hour, the medicinal material after immersion is placed in percolate pot to 75% alcohol solvent for adding in 5~6 times of medicinal material amounts, with 160~180ml/
The speed of min/kg medicinal materials carries out diacolation, collects percolate, removal solvent is up to Rhizoma Chuanxiong extract；
(3) Radix Paeoniae Alba is taken to shred, 55% ethanol wet 10~15 minutes, added in into the medicinal material after wetting 70% ethyl alcohol impregnate 10~
15 hours, the medicinal material after immersion is placed in percolate pot to 75% alcohol solvent for adding in 5~6 times of medicinal material amounts, with 160~180ml/
The speed of min/kg medicinal materials carries out diacolation, collects percolate, removal solvent is up to radix paeoniae alba extraction；
(4) Radix Ophiopogonis with 35~45 degrees Celsius of flooding twice, 1~1.5 hour every time, merge leaching liquor, filtration, filtrate concentration
To the thick paste that relative density is 1.2~1.3；
(5) 50~60% ethanol percolations of Schisandra chinensis collect percolate, recycle ethyl alcohol, are concentrated into thick paste；
(6) after Chinese cassia tree is crushed, with the extraction of 70%~85% ethanol solution twice, merge extracting solution, extracting solution is concentrated into nothing
Alcohol taste adds the water of doubling dose to dilute and stirs evenly, stands and remove sediment, obtain cinnamomum cassia extract；
(7) lycopodium calvatum, rhizoma atractylodis, Radix Glycyrrhizae, pueraria lobata, Poria cocos, rhizoma alismatis water decoct 3~4 times, 2.5~3.5 hours every time, merge and decoct
Boil liquid, filtering, filtrate are concentrated into the thick paste that relative density is 1.15~1.25, cooling, add in ethyl alcohol make alcohol content for 40%~
60%, it stands, takes supernatant, recycle ethyl alcohol, concentration and gained thick paste and extract in Chinese yam fine powder, ginsenoside, (2)-(6)
Merge, dry, adding pharmaceutic adjuvant, routinely dosage form needed for clinic is made in preparation process.
7. treatment according to claim 6 exists with the preparation method of the Chinese medicine for treating diabetes composition of lumbar vertebra disease, feature
In, the dosage form be granule, tablet, pill, capsule, injection or oral liquid, tincture, suppository, mixture, powder, lotion,
Film or dripping pill.
8. treatment according to claim 6 exists with the preparation method of the Chinese medicine for treating diabetes composition of lumbar vertebra disease, feature
In, can also be added in the dosage form ethylhydroxyethylcellulose, chitin, methylcellulose, sodium alginate, potassium alginate,
Calcium alginate, hydroxyethyl cellulose, hydroxypropyl cellulose or dextrin.
9. treatment according to claim 6 exists with the preparation method of the Chinese medicine for treating diabetes composition of lumbar vertebra disease, feature
In, the dosage form be granule, every gram of 3~6mg containing ginsenoside Re of the granule.
10. treatment according to claim 6 exists with the preparation method of the Chinese medicine for treating diabetes composition of lumbar vertebra disease, feature
In the assay method of the ginsenoside Re is：
The preparation of reference substance solution：It is appropriate that precision weighs ginsenoside Re's reference substance, and methanol is added to be respectively prepared in every 1ml and contains ginseng
The solution of saponin(e Re 0.4mg, as reference substance solution；
The preparation of test solution：This product is taken, is ground well, precision weighs 1 gram, and add methylene chloride 15ml, and water-bath flows back 0.5 hour,
Filtration, discards dichloromethane, and residue volatilizes adds the water 2ml of n-butanol saturation soak after solvent together with filter paper, precision addition water
Saturation n-butanol 40ml ultrasonic extraction 30min, precision measure supernatant, and twice times of amount of ammonification test solution shakes up, take upper solution,
Be evaporated, residue adds ethyl alcohol to dissolve, and quantitatively moves in 2ml measuring bottles, ethyl alcohol is added to be diluted to scale, shake up to get；
Chromatographic condition：It is filler with octadecylsilane chemically bonded silica；
- 0.05% phosphoric acid solution of acetonitrile that volume ratio is 50: 80 is mobile phase；Detection wavelength 203nm；Measuring method：It is accurate respectively
Reference substance solution and each 10 μ l of test solution are drawn, injects hplc determination.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108567958A (en) * | 2018-07-25 | 2018-09-25 | 杭州光启医疗科技发展有限公司 | A kind of pharmaceutical composition and preparation method thereof for treating gynaecological imflammation |
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| EP1399171A1 (en) * | 2001-05-03 | 2004-03-24 | Holomed Pharmaceuticals Ltd. | Medicinal herbal compounds for the prevention and treatment of diabetes |
| CN1616014A (en) * | 2004-09-17 | 2005-05-18 | 鲁南制药股份有限公司 | Chinese medicine composition for treating diabetes and its preparing method |
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2018
- 2018-02-27 CN CN201810163406.XA patent/CN108159258A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1399171A1 (en) * | 2001-05-03 | 2004-03-24 | Holomed Pharmaceuticals Ltd. | Medicinal herbal compounds for the prevention and treatment of diabetes |
| CN1616014A (en) * | 2004-09-17 | 2005-05-18 | 鲁南制药股份有限公司 | Chinese medicine composition for treating diabetes and its preparing method |
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| 刘文广等: "《临床骨科学新进展》", 28 February 2017, 上海世界图书出版公司 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108567958A (en) * | 2018-07-25 | 2018-09-25 | 杭州光启医疗科技发展有限公司 | A kind of pharmaceutical composition and preparation method thereof for treating gynaecological imflammation |
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Application publication date: 20180615 |
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