CN108142442A - A kind of wintergreen extract and its application as Genes For Plant Tolerance fungus - Google Patents
A kind of wintergreen extract and its application as Genes For Plant Tolerance fungus Download PDFInfo
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- CN108142442A CN108142442A CN201711324938.9A CN201711324938A CN108142442A CN 108142442 A CN108142442 A CN 108142442A CN 201711324938 A CN201711324938 A CN 201711324938A CN 108142442 A CN108142442 A CN 108142442A
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- extract
- carbendazim
- wintergreen
- bacteria
- grape
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- 239000000284 extract Substances 0.000 title claims abstract description 44
- 235000007297 Gaultheria procumbens Nutrition 0.000 title claims abstract description 27
- OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 title claims abstract description 27
- 241000196324 Embryophyta Species 0.000 title claims abstract description 15
- 241000233866 Fungi Species 0.000 title claims abstract description 8
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 7
- 241000333569 Pyrola minor Species 0.000 title 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 33
- 235000019441 ethanol Nutrition 0.000 claims abstract description 31
- 241000736246 Pyrola Species 0.000 claims abstract description 28
- 238000000034 method Methods 0.000 claims abstract description 10
- 239000000706 filtrate Substances 0.000 claims abstract description 4
- 238000002137 ultrasound extraction Methods 0.000 claims abstract description 4
- 125000003158 alcohol group Chemical group 0.000 claims abstract 2
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 28
- 239000006013 carbendazim Substances 0.000 claims description 28
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 28
- 230000002401 inhibitory effect Effects 0.000 claims description 24
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 22
- 241000894006 Bacteria Species 0.000 claims description 21
- 241000219095 Vitis Species 0.000 claims description 21
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 21
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 21
- 230000001580 bacterial effect Effects 0.000 claims description 19
- 241000193738 Bacillus anthracis Species 0.000 claims description 14
- 230000035945 sensitivity Effects 0.000 claims description 7
- 241000228212 Aspergillus Species 0.000 claims description 6
- 201000010099 disease Diseases 0.000 claims description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 6
- 241000123650 Botrytis cinerea Species 0.000 claims description 3
- 230000003385 bacteriostatic effect Effects 0.000 claims description 3
- 235000013399 edible fruits Nutrition 0.000 claims description 3
- 244000052769 pathogen Species 0.000 claims description 2
- 230000001717 pathogenic effect Effects 0.000 claims description 2
- 239000000575 pesticide Substances 0.000 abstract description 8
- 240000000560 Citrus x paradisi Species 0.000 abstract description 3
- 230000006837 decompression Effects 0.000 abstract description 3
- 241001465754 Metazoa Species 0.000 abstract description 2
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 2
- 230000000845 anti-microbial effect Effects 0.000 abstract description 2
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 238000001228 spectrum Methods 0.000 abstract description 2
- 239000003814 drug Substances 0.000 description 4
- 230000002265 prevention Effects 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000208421 Ericaceae Species 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 244000061520 Angelica archangelica Species 0.000 description 1
- 241000125121 Aspergillus carbonarius Species 0.000 description 1
- 241001465180 Botrytis Species 0.000 description 1
- 241000688200 Cingulata Species 0.000 description 1
- 241000326334 Coniella diplodiella Species 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241001620302 Glomerella <beetle> Species 0.000 description 1
- 235000001287 Guettarda speciosa Nutrition 0.000 description 1
- STECJAGHUSJQJN-GAUPFVANSA-N Hyoscine Natural products C1([C@H](CO)C(=O)OC2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-GAUPFVANSA-N 0.000 description 1
- 231100000678 Mycotoxin Toxicity 0.000 description 1
- STECJAGHUSJQJN-UHFFFAOYSA-N N-Methyl-scopolamin Natural products C1C(C2C3O2)N(C)C3CC1OC(=O)C(CO)C1=CC=CC=C1 STECJAGHUSJQJN-UHFFFAOYSA-N 0.000 description 1
- 241001486560 Pyrola calliantha Species 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 240000006365 Vitis vinifera Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960000074 biopharmaceutical Drugs 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000006229 carbon black Substances 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000001408 fungistatic effect Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 239000002636 mycotoxin Substances 0.000 description 1
- 210000003739 neck Anatomy 0.000 description 1
- 244000039328 opportunistic pathogen Species 0.000 description 1
- 235000017807 phytochemicals Nutrition 0.000 description 1
- 229930000223 plant secondary metabolite Natural products 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- STECJAGHUSJQJN-FWXGHANASA-N scopolamine Chemical compound C1([C@@H](CO)C(=O)O[C@H]2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-FWXGHANASA-N 0.000 description 1
- 229960002646 scopolamine Drugs 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000007711 solidification Methods 0.000 description 1
- 230000008023 solidification Effects 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000007920 subcutaneous administration Methods 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Plant Pathology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
Abstract
The present invention relates to a kind of wintergreen extract, the wintergreen extract is alcohol extract, and wherein alcohol extract extracting method is：It is to weigh to crush sample 800g, impregnates plant drymeal with the ethyl alcohol of 5 times of amounts, stand 12h at room temperature；Ultrasonic extraction 2h；Decompression filters, and continuous to extract 3 times, merging filtrate is concentrated at 45 DEG C with Rotary Evaporators, after dry, preserved in 4 DEG C, is spare；Further include application of the wintergreen extract as Genes For Plant Tolerance fungus.Wintergreen extract used in the present invention is a kind of botanical biological pesticide, has the characteristics that antibacterial activity is high, antimicrobial spectrum is wide, is not easy to produce resistance, is nontoxic to humans and animals, can improve grape fruit foodsafety.
Description
Technical field
The present invention relates to field of biological pesticide, and in particular to botanical biological pesticide is more particularly to a kind of wintergreen and carries
Take object and its application as Genes For Plant Tolerance fungus.
Background technology
Grape grey mould, white rot, anthracnose etc. seriously affect grape quality and yield, not still in the production of China's grape
The difficulty that must be solved, and be also the problem in worldwide grape production.In agricultural production, it is useization to commonly use preventing control method
It learns to farm the chemical prevention method of medicine, but chemical prevention has drug resistance, again residual contamination, the wind such as wildness for causing grape pathogen
Danger.Using a variety of methods, alternately cooperation Synthetical prevention is pest and disease damage prevention and control and prevents the developing direction that mycotoxin pollutes and avoid
The approach of risk.Therefore other approaches and methods of non-chemical pesticide are found, are that the vital task of industry development and science are ground
The important topic studied carefully.Plant source method is excavated from plant, is found first including exploitation botanical pesticide, from phytochemical
The direct utilization of compound, even plant leaching liquor immersion is led, is the important content of this developing direction.
Wintergreen (Pyrola calliantha H.Andr.) is Pyrolaceae (Pyrolaceae) pyrola
(Pyrola) plant, production Shaanxi, Qinghai, Gansu, Shanxi, Shandong, Hebei, Henan, Anhui, Jiangsu, Zhejiang, Fujian, Hubei, lake
South, Jiangxi, Sichuan, Guizhou, Yunnan, Tibet.It is born in 700-4100 meters of needle leaf forests of height above sea level, mixed coniferous broad leaved forest or broad-leaf forest
Under.This kind of herb hyoscine makees astringent, civil to be used as tonic, controls consumptive disease, cough-relieving, strengthening the muscles and bones.But it has no as life
Report and research of the object pesticides application to preventing and treating grapevine diseases.
Invention content
Answering the invention mainly solves the technical problem of providing a kind of wintergreen extract and its as Genes For Plant Tolerance fungus
With grape foodsafety can be improved.
In order to solve the above technical problems, the technical solution adopted by the present invention is：
A kind of wintergreen extract, the wintergreen extract are alcohol extract, and the alcohol extract extracting method is：It is
It weighs and crushes sample 800g, impregnate plant drymeal with the ethyl alcohol of 5 times of amounts, stand 12h at room temperature；Ultrasonic extraction 2h；Decompression filters,
Continuous to extract 3 times, merging filtrate is concentrated at 45 DEG C with Rotary Evaporators, after dry, preserved in 4 DEG C, is spare.
A kind of application of wintergreen extract as Genes For Plant Tolerance fungus is further related to, the wintergreen extract is to following disease
Opportunistic pathogen of falling ill has significant bacteriostatic activity, mainly including Botrytis cinerea, fruit white rot of grape bacterium, grape anthracnose and carbon black
Aspergillus germ.
Further, wherein the grape anthracnose is included to carbendazim highly resistance anthrax bacteria bacterial strain, the grey mold
Germ is included to carbendazim highly resistance ash arrhizus bacteria bacterial strain.
Further, a concentration of 0.3125mg/mL-5mg/mL of the alcohol extract.
Further, as a concentration of 5mg/mL of alcohol extract, to anthrax bacteria, white rot germ, ash arrhizus bacteria and Aspergillus carbonerius
Germ and inhibiting rate are respectively 45.99%, 36.75%, 24.59% and 30.63%, to carbendazim sensitivity anthrax bacteria bacterial strain
The inhibiting rate of FJZZ-62, coll-9 and coll-20 are respectively 66.67%, 68.55% and 57.81%, to carbendazim highly resistance charcoal
The inhibiting rate of subcutaneous ulcer germ bacterial strain FJND-9, FJND-40 and GZSD-89 are respectively 56.09%, 76.55% and 54.71%, to more
The inhibiting rate of sensitive sense grape grey mould bacteria strain GXGL-7, GXNN-6 and the GXNN-7 of bacterium is respectively 42.28%, 44.79% and
55.22%, the inhibiting rate to carbendazim highly resistance grape grey mould bacteria strain HBLF-10, GXZY-4 and GXZY-7 is respectively
44.83%th, 61.00% and 57.38%.
The beneficial effects of the invention are as follows：Wintergreen extract of the present invention is one as the germ killing drugs of active ingredient
Kind botanical biological pesticide, belongs to one kind of bio-pharmaceutical, meets requirement of the modern society to novel pesticide.Secondly, wintergreen carries
Object is taken to have the characteristics that antibacterial activity is high, antimicrobial spectrum is wide, be not easy to produce resistance, be nontoxic to humans and animals.In addition, it is to grape fruit
Real encountered pathogenic bacteria has inhibition, can improve grape fruit foodsafety.
Specific embodiment
It is described in detail with reference to preferred embodiment, so that advantages and features of the invention can be easier to by this field
Technical staff's understanding, so as to make a clearer definition of the protection scope of the present invention.
Embodiment 1：
First, the preparation of alcohol extract
The wintergreen of the present invention is purchased from Hui nationality's medicinal material market.Lay-by material is dried, crushes, is placed at aeration-drying
It saves backup.It weighs and crushes sample 800g, impregnate plant drymeal with the ethyl alcohol of 5 times of amounts, stand 12h at room temperature；Ultrasonic extraction 2h；
Decompression filters, and continuous to extract 3 times, merging filtrate is concentrated at 45 DEG C with Rotary Evaporators, after dry, preserved in 4 DEG C, is spare.
2nd, the bacteriostasis of alcohol extract
Root of Dahurain angelica water extract, alcohol extract are measured to Botrytis cinerea (Botrytis using mycelial growth rate method
Cinerea), fruit white rot of grape bacterium (Coniothyrium diplodiella), grape anthracnose (Glomerella
) and the bacteriostatic activity of Aspergillus carbonerius (Aspergillus carbonarius) cingulata.It is as follows：
Strain will be frozen to recover at room temperature, with a small amount of bacterium colony picking of transfer needle picking to the culture dish containing PDA culture medium
Central area, sealing, no light inversion are incubated in the incubator of 25 DEG C of temperature, humidity 73%.
A certain amount of extract is weighed, alcohol extract is configured to 50mg/mL, 25mg/mL, 12.5mg/ respectively with sterile water
The solution of mL, 6.25mg/mL, 3.125mg/mL.Sterile water is as blank control, and the carbendazim of 5 μ g/mL is as positive control.
In aseptic workbench, by prepared liquid respectively with PDA culture medium according to 1:9 ratio mixing, makes band medicine culture
A concentration of 5mg/mL, 2.5mg/mL, 1.25mg/mL, 0.625mg/mL, 0.3125mg/mL of base, pour into a diameter of 90mm's
Its solidification is treated in culture dish, placement.
After actication of culture, vigorous place is grown in mycelia with aseptic card punch (Ф=5mm) and punched, bacteria cake, inoculation is made
Onto band medicine culture medium, each culture dish center connects a bacteria cake, is 25-28 DEG C in temperature, humidity is in 73% incubator
After being inverted culture 2-7d, colony diameter is surveyed with crossing method, calculates inhibiting rate.Each processing sets 3 repetitions.
The result shows that：Wintergreen alcohol extract shows different degrees of fungistatic effect to 4 kinds of grape disease fungus, works as alcohol extracting
During a concentration of 5mg/mL of object, it is respectively to anthrax bacteria, white rot germ, ash arrhizus bacteria and Aspergillus carbonerius germ and inhibiting rate
45.99%th, 36.75%, 24.59% and 30.63%.
3rd, alcohol extract is to the inhibiting effect of carbendazim sensitivity anthrax bacteria bacterial strain
As a concentration of 5mg/mL of wintergreen alcohol extract, to carbendazim sensitivity anthrax bacteria bacterial strain FJZZ-62, coll-9 and
The inhibiting rate of coll-20 is respectively 66.67%, 68.55% and 57.81%.
4th, alcohol extract is to the inhibiting effect of carbendazim highly resistance anthrax bacteria bacterial strain
100 μ g/mL carbendazim are respectively 9.80% to the inhibiting rate of bacterial strain FJND-9, FJND-40 and GZSD-89,
21.50% and 10.01%.Wintergreen alcohol extract has stronger inhibition to carbendazim highly resistance anthrax bacteria bacterial strain.When wintergreen alcohol
During a concentration of 5mg/mL of extract, to the inhibiting rate point of carbendazim highly resistance anthrax bacteria bacterial strain FJND-9, FJND-40 and GZSD-89
Not Wei 56.09%, 76.55% and 54.71% (table 3), effect be substantially better than 100 μ g/mL carbendazim.0.625mg/mL alcohol extractings
Object is suitable with the effect of 100 μ g/mL carbendazim to the inhibiting effect of carbendazim highly resistance anthrax bacteria bacterial strain.
5th, alcohol extract is to the inhibiting effect of carbendazim sensitivity ash arrhizus bacteria bacterial strain
As a concentration of 5mg/mL of wintergreen alcohol extract, to carbendazim sensitivity grape grey mould bacteria strain GXGL-7, GXNN-
The inhibiting rate of 6 and GXNN-7 is respectively 42.28%, 44.79% and 55.22%.
6th, alcohol extract is to the inhibiting effect of carbendazim highly resistance ash arrhizus bacteria bacterial strain
100 μ g/mL carbendazim are respectively 53.45% to the inhibiting rate of bacterial strain HBLF-10, GXZY-4 and GXZY-7,
55.11% and 14.76%.As a concentration of 5mg/mL of wintergreen alcohol extract, to carbendazim highly resistance grape grey mould bacteria strain
The inhibiting rate of HBLF-10, GXZY-4 and GXZY-7 are respectively 44.83%, 61.00% and 57.38%.Wintergreen alcohol extract
(5mg/mL) is less than 100 μ g/mL carbendazim to the inhibiting effect of bacterial strain HBLF-10, and the inhibition of GXZY-4 and GXZY-7 is made
With being better than 100 μ g/mL carbendazim.
The foregoing is merely the embodiment of the present invention, are not intended to limit the scope of the invention, every to utilize this hair
The equivalent structure or equivalent flow shift that bright description is made directly or indirectly is used in other relevant technology necks
Domain is included within the scope of the present invention.
Claims (5)
1. a kind of wintergreen extract, it is characterised in that：The wintergreen extract is alcohol extract, and the alcohol extract extracts
Method is：It weighs and crushes sample 800g, impregnate plant drymeal with the ethyl alcohol of 5 times of amounts, stand 12h at room temperature；Ultrasonic extraction 2h；Subtract
Pressure filters, and continuous to extract 3 times, merging filtrate is concentrated at 45 DEG C with Rotary Evaporators, after dry, preserved in 4 DEG C, is spare.
2. application of a kind of wintergreen extract as described in claim 1 as Genes For Plant Tolerance fungus, which is characterized in that described
Wintergreen extract has significant bacteriostatic activity to following disease pathogen, mainly including Botrytis cinerea, fruit white rot of grape
Bacterium, grape anthracnose and Aspergillus carbonerius germ.
3. application as claimed in claim 2, which is characterized in that the wherein described grape anthracnose is included to carbendazim highly resistance
Anthrax bacteria bacterial strain, the ash arrhizus bacteria are included to carbendazim highly resistance ash arrhizus bacteria bacterial strain.
4. application as claimed in claim 2 or claim 3, which is characterized in that a concentration of 0.3125mg/mL-5mg/ of the alcohol extract
mL。
5. application as claimed in claim 4, which is characterized in that as a concentration of 5mg/mL of alcohol extract, to anthrax bacteria, white rot
Germ, ash arrhizus bacteria and Aspergillus carbonerius germ and inhibiting rate are respectively 45.99%, 36.75%, 24.59% and 30.63%, right
The inhibiting rate of carbendazim sensitivity anthrax bacteria bacterial strain FJZZ-62, coll-9 and coll-20 are respectively 66.67%, 68.55% and
57.81%, the inhibiting rate to carbendazim highly resistance anthrax bacteria bacterial strain FJND-9, FJND-40 and GZSD-89 is respectively 56.09%,
76.55% and 54.71%, to the inhibiting rate point of carbendazim sensitivity grape grey mould bacteria strain GXGL-7, GXNN-6 and GXNN-7
Not Wei 42.28%, 44.79% and 55.22%, to carbendazim highly resistance grape grey mould bacteria strain HBLF-10, GXZY-4 and
The inhibiting rate of GXZY-7 is respectively 44.83%, 61.00% and 57.38%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711324938.9A CN108142442B (en) | 2017-12-13 | 2017-12-13 | Wintergreen extract and application thereof as plant antibacterial material |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711324938.9A CN108142442B (en) | 2017-12-13 | 2017-12-13 | Wintergreen extract and application thereof as plant antibacterial material |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108142442A true CN108142442A (en) | 2018-06-12 |
| CN108142442B CN108142442B (en) | 2020-10-30 |
Family
ID=62467064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711324938.9A Expired - Fee Related CN108142442B (en) | 2017-12-13 | 2017-12-13 | Wintergreen extract and application thereof as plant antibacterial material |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108142442B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113773970A (en) * | 2021-10-14 | 2021-12-10 | 四川农业大学 | Aspergillus niger BZZJ-17 with plant growth promoting effect |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101401591A (en) * | 2008-11-24 | 2009-04-08 | 王占斌 | Method for extracting bactericide from wintergreen |
| CN101862028A (en) * | 2010-06-09 | 2010-10-20 | 广东神农烟科技术有限公司 | Wintergreen extraction method, cigarette comprising same and preparation method thereof |
-
2017
- 2017-12-13 CN CN201711324938.9A patent/CN108142442B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101401591A (en) * | 2008-11-24 | 2009-04-08 | 王占斌 | Method for extracting bactericide from wintergreen |
| CN101862028A (en) * | 2010-06-09 | 2010-10-20 | 广东神农烟科技术有限公司 | Wintergreen extraction method, cigarette comprising same and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 王亚飞 等: "鹿蹄草等4种中草药提取液对桃褐腐菌抑制作用研究", 《安徽农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113773970A (en) * | 2021-10-14 | 2021-12-10 | 四川农业大学 | Aspergillus niger BZZJ-17 with plant growth promoting effect |
| CN113773970B (en) * | 2021-10-14 | 2023-05-23 | 四川农业大学 | Aspergillus niger BZZJ-17 with plant growth promoting effect |
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| Publication number | Publication date |
|---|---|
| CN108142442B (en) | 2020-10-30 |
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