CN108142285B - In-vitro rapid propagation culture method of Lujing - Google Patents
In-vitro rapid propagation culture method of Lujing Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
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Abstract
The invention relates to an in vitro rapid propagation culture method of exposed lens, belonging to the technical field of plant tissue culture, selecting a stem crop explant with a lateral bud or a terminal bud, sterilizing the surface with alcohol and mercury bichloride to obtain a sterile plant, and obtaining a complete plant of exposed lens through induction, proliferation, rooting culture and hardening seedling transplantation; the invention obtains the seedling of the bare glass by carrying out tissue culture on the stem section of the bare glass, and the method has the advantages of easily obtained materials, convenient disinfection, high survival rate and short production period, and greatly improves the propagation coefficient and the propagation speed of the bare glass; secondly, the invention firstly realizes the in vitro rapid propagation culture of the plant Lusciola of the family nettle, and lays a foundation for the tissue culture industrialization and large-scale production system of the plant Lusciola.
Description
Technical Field
The invention belongs to the technical field of plant tissue culture, and particularly relates to an in-vitro rapid propagation culture method of bare glass.
Background
Exposed mirror (Pilea serpyllacea'Globosa'), cold water flower of nettle, dioecious male, very small female flower, 3-split crown, purplish red; the male flower is 3.5mm bigger, the crown is 4 fissures, and is white. The green branches have fleshy and most of the branches, and the fine leaves are scattered on the branches and generally form a group at the tops of the branches. The main point of view of the open mirror is also at its natantide leafThe ellipsoidal leaves have green spherical upper surface and semi-spherical lower surface, and are like a drop of crystal-clear dew. Under strong light irradiation, the stem and leaf surface become beautiful reddish purple with glittering and translucent luster. The purple upper cuticle of the leaf and the stalactite in the mesophyll tissue protect the leaf from the intense ultraviolet rays, while the translucent lower surface helps absorb some of the light reflected from the ground. High altitude areas (venezuela, borlivia, columbia, ecuador, peru) native to the west of south america like humus soil with sufficient moisture, open-mirror maintenance is difficult, on the one hand, it likes an environment with large humidity, even can be semi-aquatic; on the other hand, it is not resistant to hot and humid weather, so an environment with sufficient illumination and good ventilation is created in summer.
At present, the open lens maintenance is more in Japan, and people who are maintained in China can have a certain number of fingers. The bare-lens propagation generally adopts a cutting mode, and the propagation speed is low; the seeds are difficult to propagate, the male and female plants are different, the flowers are very small, and the pollination is difficult. The exposed lens leaves have unique ornamental value, are small and exquisite, are deeply loved by broad enthusiasts and suffer from market scarcity, so the exposed lens leaves have potential market prospect and economic value.
Disclosure of Invention
In order to overcome the problems in the background art, the invention provides an in-vitro rapid propagation and culture method of the bare glass, a good bare glass group culture and regeneration system is established, compared with the traditional propagation mode, the method is not limited by seasons, materials and environments, the environment required by plants is manually controlled, the method can be produced all the year round, the problems of single bare glass propagation mode, low speed, scarce market and the like are solved, and the technical support is provided for industrialization and marketization of bare glass seedlings.
In order to realize the purpose, the invention is realized by the following technical scheme:
the in-vitro rapid propagation culture method of the bare lens specifically comprises the following steps:
(1) and (3) disinfection treatment of explants: selecting explants, washing the surfaces of the explants with running tap water, soaking the explants for 30s with 75% alcohol on an ultraclean workbench, washing the explants with sterile water for 3-4 times, then adding 0.1% mercuric chloride, dripping 2 drops of Tween, and disinfecting for 5-8min, oscillating at intervals, washing the explants with sterile water for 4-5 times, sucking water with sterile filter paper, and cutting off the cut to disinfect the injured parts;
(2) and (3) induction culture: inoculating the sterile explant obtained in the step (1) into an induction culture medium MS +0.6-0.8mg/L6-BA +0.05-0.2mg/L LNAA +20-30g/L sugar +7g/L agar, wherein the pH value is 5.8-6.0, and after induction culture is carried out for 14-21d, the base part of the explant grows out cluster buds;
(3) and (3) proliferation culture: 2-3 cluster buds formed in the step (2) are used as a cluster, inoculated into a proliferation culture medium MS +0.4-0.6mg/L6-BA +0.01-0.1mg/L LNAA +0.1-0.2mg/L paclobutrazol +20-30g/L sugar +7g/L agar, the pH value is 5.8-6.0, after proliferation culture for 4-6 weeks, proliferation is carried out for 3-4 times, and the plant height of the cluster buds is up to 1.5-2 cm;
(4) rooting culture: cutting the cluster seedlings obtained in the step (3) into 2-3 plants, inoculating the 2-3 plants into a rooting culture medium MS +0.05-0.1mg/L6-BA +0.5-1.0mg/L LNAA +0.1-0.2mg/L paclobutrazol +20-30g/L sugar +7g/L agar, controlling the pH value to be 5.8-6.0, and germinating after rooting culture for 4-6 weeks;
(5) hardening and transplanting seedlings: and (3) transplanting the rooted seedlings obtained in the step (4) to a greenhouse for acclimatization for 5-7d, lightly taking out tissue culture bottle seedlings by using forceps, soaking the whole plants in 800-1000 times of 50% carbendazim wettable powder for 10min, taking out, airing water, planting the seedlings in a matrix, shading 75-85%, and reducing humidity by 60-80%, culturing for 10-14d, and then gradually increasing illumination and reducing humidity to obtain the complete plants with exposed lenses.
Further, in the step (1), the explant is a 1cm young shoot with lateral buds or terminal buds.
Further, in the steps (2) to (4), the culture conditions are that the temperature is 20 +/-2 ℃, the illumination intensity is 3000-5000 lx, the illumination is 12h/d, and the relative humidity is 50% -60%.
Further, in the step (5), after the tissue culture bottle seedlings are gently taken out by using tweezers, roots of the rooted seedlings are cut off by using sterilized scissors.
Further, in the step (5), the matrix comprises vermiculite, humus soil and perlite, and the ratio of the vermiculite to the humus soil to the perlite is 1:1: 1.
The invention has the beneficial effects that:
according to the invention, by establishing the tissue culture rapid propagation system of the bare glass, the production is not limited by regions, seasons and climates, the disinfection survival rate of explants can reach more than 65%, the inductivity reaches more than 95%, the propagation coefficient reaches 3-4 times, the rooting rate is 100%, and the transplanting survival rate is more than 90%, so that the industrial seedling raising is facilitated, a foundation is laid for the tissue culture industrialization and large-scale production system of bare glass plants, and a theoretical basis is provided for the tissue culture rapid propagation system of other plant varieties.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, preferred embodiments of the present invention will be described in detail below to facilitate understanding of the skilled person.
Example 1
(1) And (3) disinfection treatment of explants: selecting young twigs with exposed lens, cutting into 1cm long stem segments with lateral buds or terminal buds as explants, washing the surface with running tap water, soaking in 75% alcohol for 30s on a superclean bench, washing with sterile water for 3-4 times, adding 0.1% mercuric chloride (mass ratio), dripping 2 drops of Tween for disinfection for 5-8min, oscillating at intervals, washing with sterile water for 4-5 times, sucking water with sterile filter paper, cutting off the cut, disinfecting and injuring, and inoculating into an induction culture medium. After 1 week, the number of the survival seedlings is counted, the pollution rate is 10.5%, and the survival rate reaches more than 65%.
(2) And (3) induction culture: inoculating the sterile explant obtained in the step (1) into an induction culture medium MS +0.6-0.8mg/L6-BA +0.05-0.2mg/L LNAA +20-30g/L sugar +7g/L agar, carrying out induction culture at the pH value of 5.8-6.0 at the temperature of 20 +/-2 ℃, under the conditions of illumination intensity of 3000-5000 lx, illumination of 12h/d and relative humidity of 50-60%, wherein the base part starts to bud and expand after being cultured for 5-7d, and after being cultured for 14-21d, the base part of the explant grows out cluster buds. After 3 weeks, the statistical induction rate reaches more than 95%.
(3) And (3) proliferation culture: and (3) taking 2-3 cluster buds formed in the step (2) as a cluster, inoculating the cluster buds into a proliferation culture medium MS +0.4-0.6mg/L6-BA +0.01-0.1mg/L LNAA +0.1-0.2mg/L paclobutrazol +20-30g/L sugar +7g/L agar, carrying out proliferation culture at the pH value of 5.8-6.0 under the conditions of the temperature of 20 +/-2 ℃, the illumination intensity of 3000-5000 lx, the illumination of 12h/d and the relative humidity of 50-60%, and after culturing for 4-6 weeks, proliferating to 3-4 times, wherein the plant height of the cluster buds is 1.5-2 cm.
(4) Rooting culture: cutting the cluster seedlings obtained in the step (3) into 2-3 plants, inoculating the 2-3 plants into a rooting culture medium MS +0.05-0.1mg/L6-BA +0.5-1.0mg/L LNAA +0.1-0.2mg/L paclobutrazol +20-30g/L sugar +7g/L agar, carrying out rooting culture under the conditions that the pH value is 5.8-6.0, the temperature is 20 +/-2 ℃, the illumination intensity is 3000-5000 lx, the illumination is 12h/d and the relative humidity is 50-60%, rooting is started after 1-2 weeks of culture, seedlings can emerge after 4-6 weeks of culture, the rooting rate is 100%, the root length is 1.5-2cm, and the plant height is 3-4 cm;
(5) hardening and transplanting seedlings: and (3) transplanting the rooted seedlings obtained in the step (4) to a greenhouse for acclimatization for 5-7d, lightly taking out tissue culture bottle seedlings by using forceps, taking out the seedlings, shearing off roots by using sterilized scissors, soaking the seedlings in 800-1000 times of 50% carbendazim wettable powder for 10min, taking out the seedlings, drying the seedlings in the air, planting the seedlings in a matrix (humus soil: perlite =1: 1), shading light by 75-85% and humidity by 60-80%, culturing for 10-14d, gradually increasing the light and reducing the humidity to obtain mirror-exposed complete plants, and culturing in a normal greenhouse. After 4 weeks, the survival rate reaches more than 90 percent.
Example 2
(1) And (3) disinfection treatment of explants: selecting young twigs with exposed lens, cutting into 1cm long stem segments with lateral buds or terminal buds as explants, washing the surface with running tap water, soaking in 75% alcohol for 30s on a superclean bench, washing with sterile water for 3-4 times, sterilizing with 5% by volume sodium hypochlorite for 8-10min, shaking occasionally, washing with sterile water for 4-5 times, sucking water with sterile filter paper, cutting off the cut to sterilize the injured part, and inoculating into an induction culture medium. After 1 week, the number of the survival seedlings is counted, the pollution rate is 82.6 percent, and the survival rate reaches 70.4 percent.
(2) And (3) induction culture: inoculating the sterile explant obtained in the step (1) into an induction culture medium MS +0.6-0.8mg/L6-BA +0.05-0.2mg/L LNAA +20-30g/L sugar +7g/L agar, carrying out induction culture at the pH value of 5.8-6.0 at the temperature of 20 +/-2 ℃, under the conditions of illumination intensity of 3000-5000 lx, illumination of 12h/d and relative humidity of 50-60%, wherein the base part starts to bud and expand after being cultured for 5-7d, and after being cultured for 14-21d, the base part of the explant grows out cluster buds. After 3 weeks, the induction rate reaches 95%.
(3) And (3) proliferation culture: and (3) taking 2-3 cluster buds formed in the step (2) as a cluster, inoculating the cluster buds into a proliferation culture medium MS +0.4-0.6mg/L6-BA +0.01-0.1mg/L LNAA +0.05-0.1mg/L paclobutrazol +20-30g/L sugar +7g/L agar, carrying out proliferation culture at the pH value of 5.8-6.0 under the conditions of the temperature of 20 +/-2 ℃, the illumination intensity of 2000-3000 lx, illumination of 12h/d and the relative humidity of 50-60%, and after culturing for 4-6 weeks, proliferating to 2-3 times, and growing the plant height of the cluster buds to 3-5.5 cm.
(4) Rooting culture: cutting the cluster seedlings obtained in the step (3) into 2-3 plants, inoculating the 2-3 plants into a rooting culture medium MS +0.05-0.1mg/L6-BA +0.5-1.0mg/L LNAA +0.05-0.1mg/L paclobutrazol +20-30g/L sugar +7g/L agar, carrying out rooting culture under the conditions that the pH value is 5.8-6.0, the temperature is 20 +/-2 ℃, the illumination intensity is 3000-5000 lx, the illumination is 12h/d and the relative humidity is 50-60%, rooting is started after 1-2 weeks of culture, seedlings can emerge after 4-6 weeks of culture, the rooting rate is 100%, the root length is 1.5-2cm, and the plant height is 4-5 cm;
(5) hardening and transplanting seedlings: transplanting the rooted seedlings obtained in the step (4) to a greenhouse for acclimatization for 5-7d, lightly taking out tissue culture bottle seedlings by using forceps, taking out the seedlings, shearing off roots by using sterilized scissors, soaking the seedlings in 800-1000 times of 50% carbendazim wettable powder for 10min, taking out, drying the seedlings in the air, planting the seedlings in a matrix (vermiculite: humus soil: perlite =1:1: 1), shading the light by 75-85% and the humidity by 60-80%, culturing for 20d, gradually increasing the light and reducing the humidity to obtain the complete plants with exposed lenses, and culturing in the normal greenhouse. After 4 weeks, the survival rate reaches more than 95 percent.
Example 3
(1) And (3) disinfection treatment of explants: selecting young twigs with exposed lens, cutting into 1cm long stem segments with lateral buds or terminal buds as explants, washing the surface with running tap water, soaking in 75% alcohol for 30s on a superclean bench, washing with sterile water for 3-4 times, adding 0.1% mercuric chloride (mass ratio), dripping 2 drops of Tween for disinfection for 5-8min, oscillating at intervals, washing with sterile water for 4-5 times, sucking water with sterile filter paper, cutting off the cut, disinfecting and injuring, and inoculating into an induction culture medium. After 1 week, the number of the survival seedlings is counted, the pollution rate is 10.5%, and the survival rate reaches more than 65%.
(2) And (3) induction culture: inoculating the sterile explant obtained in the step (1) into an induction culture medium MS +0.6-0.8mg/L6-BA +0.05-0.2mg/L LNAA +20-30g/L sugar +7g/L agar, carrying out induction culture at the pH value of 5.8-6.0 at the temperature of 20 +/-2 ℃, the illumination intensity of 2000-3000 lx, illumination for 12h/d and the relative humidity of 50-60%, wherein the base part of the explant starts to bud and expand after being cultured for 5-7d, and the base part of the explant grows out cluster buds after being cultured for 14-21 d. After 3 weeks, the statistical induction rate reaches more than 95%.
(3) And (3) proliferation culture: and (3) taking 2-3 cluster buds formed in the step (2) as a cluster, inoculating the cluster buds into a proliferation culture medium MS +0.4-0.6mg/L6-BA +0.01-0.1mg/L LNAA +0.1-0.2mg/L paclobutrazol +20-30g/L sugar +7g/L agar, carrying out proliferation culture at the pH value of 5.8-6.0 under the conditions of the temperature of 20 +/-2 ℃, the illumination intensity of 2000-3000 lx, illumination of 12h/d and the relative humidity of 50-60%, and after culturing for 5-6 weeks, proliferating to 2 times, wherein the plant height of the cluster buds is 2-4 cm.
(4) Rooting culture: cutting the cluster seedlings obtained in the step (3) into 2-3 plants, inoculating the 2-3 plants into a rooting culture medium MS +0.05-0.1mg/L6-BA +0.5-1.0mg/LNAA +0.1-0.2mg/L paclobutrazol +20-30g/L sugar +7g/L agar, carrying out rooting culture under the conditions that the pH value is 5.8-6.0, the temperature is 20 +/-2 ℃, the illumination intensity is 2000-3000 lx, the illumination is 12h/d and the relative humidity is 50-60%, starting rooting after 1-2 weeks of culture, and emerging seedlings can emerge after 4-6 weeks of culture, the rooting rate is 100%, the root length is 2-3cm, and the plant height is 4-5 cm;
(5) hardening and transplanting seedlings: and (3) transplanting the rooted seedlings obtained in the step (4) to a greenhouse for acclimatization for 5-7d, lightly taking out tissue culture bottle seedlings by using forceps, cleaning a culture medium on the surface of the plants by using clean water after taking out, soaking the whole plants by using 50% carbendazim wettable powder 800-1000 times for 10min, taking out, drying the plants in the air, planting the plants in a matrix (humus soil: perlite =1: 1), shading 75-85% and humidity 60-80%, culturing for 10-14d, gradually increasing light and reducing humidity to obtain the complete plants with exposed lenses, and culturing in a normal greenhouse. After 4 weeks, the survival rate reaches more than 70 percent.
Example 4
(1) And (3) disinfection treatment of explants: selecting young twigs with exposed lens, cutting into 1cm long stem segments with lateral buds or terminal buds as explants, washing the surface with running tap water, soaking in 75% alcohol for 30s on a superclean bench, washing with sterile water for 3-4 times, adding 0.1% mercuric chloride (mass ratio), dripping 2 drops of Tween for disinfection for 5-8min, oscillating at intervals, washing with sterile water for 4-5 times, sucking water with sterile filter paper, cutting off the cut, disinfecting and injuring, and inoculating into an induction culture medium. After 1 week, the number of the survival seedlings is counted, the pollution rate is 10.5%, and the survival rate reaches more than 65%.
(2) And (3) induction culture: inoculating the sterile explant obtained in the step (1) into an induction culture medium MS +0.6-0.8mg/L6-BA +0.05-0.2mg/L LNAA +20-30g/L sugar +7g/L agar, carrying out induction culture at the pH value of 5.8-6.0 at the temperature of 20 +/-2 ℃, under the conditions of illumination intensity of 3000-5000 lx, illumination of 12h/d and relative humidity of 50-60%, wherein the base part starts to bud and expand after being cultured for 5-7d, and after being cultured for 14-21d, the base part of the explant grows out cluster buds. After 3 weeks, the statistical induction rate reaches more than 95%.
(3) And (3) proliferation culture: and (3) taking 2-3 cluster buds formed in the step (2) as a cluster, inoculating the cluster buds into a proliferation culture medium MS +0.4-0.6mg/L6-BA +0.01-0.1mg/L LNAA +0.1-0.2mg/L paclobutrazol +20-30g/L sugar +7g/L agar, carrying out proliferation culture at the pH value of 5.8-6.0 under the conditions of the temperature of 20 +/-2 ℃, the illumination intensity of 3000-5000 lx, the illumination of 12h/d and the relative humidity of 50-60%, and after culturing for 4-6 weeks, proliferating to 3-4 times, wherein the plant height of the cluster buds is 1.5-2 cm.
(4) Rooting culture: cutting the cluster seedlings obtained in the step (3) into 2-3 plants, inoculating the 2-3 plants into a rooting culture medium MS +0.05-0.1mg/L6-BA +0.5-1.0mg/L LNAA +0.1-0.2mg/L paclobutrazol +20-30g/L sugar +7g/L agar, carrying out rooting culture under the conditions that the pH value is 5.8-6.0, the temperature is 20 +/-2 ℃, the illumination intensity is 3000-5000 lx, the illumination is 12h/d and the relative humidity is 50-60%, rooting is started after 1-2 weeks of culture, seedlings can emerge after 4-6 weeks of culture, the rooting rate is 100%, the root length is 1.5-2cm, and the plant height is 3-4 cm;
(5) hardening and transplanting seedlings: transplanting the rooted seedlings obtained in the step (4) to a greenhouse for acclimatization for 5-7d, lightly taking out tissue culture bottle seedlings by using forceps, taking out the seedlings, shearing off roots by using sterilized scissors, soaking the seedlings in 800-1000 times of 50% carbendazim wettable powder for 10min, taking out, drying the seedlings in the air, planting the seedlings in a matrix (vermiculite: humus soil: perlite =1:1: 1), shading the light by 75-85% and the humidity by 60-80%, culturing for 20d, gradually increasing the light and reducing the humidity to obtain the complete plants with exposed lenses, and culturing in the normal greenhouse. After 4 weeks, the survival rate reaches more than 95 percent.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (4)
1. An in vitro rapid propagation culture method of bare lens is characterized in that: the method specifically comprises the following steps:
(1) and (3) disinfection treatment of explants: the explant is 1cm of young stem with lateral bud or terminal bud, the explant is selected to be washed with running tap water on the surface, soaked in 75% alcohol for 30s on a super clean bench, washed with sterile water for 3-4 times, then 0.1% mercuric chloride is added, 2 drops of tween is dropped for disinfection for 5-8min, the explant is oscillated from time to time, washed with sterile water for 4-5 times, the water is absorbed by sterile filter paper, and the part of the cut which is injured by disinfection is cut off;
(2) and (3) induction culture: inoculating the sterile explant obtained in the step (1) into an induction culture medium MS +0.6-0.8mg/L6-BA +0.05-0.2mg/L LNAA +20-30g/L sugar +7g/L agar, wherein the pH value is 5.8-6.0, and after induction culture is carried out for 14-21d, the base part of the explant grows out cluster buds;
(3) and (3) proliferation culture: 2-3 cluster buds formed in the step (2) are used as a cluster, inoculated into a proliferation culture medium MS +0.4-0.6mg/L6-BA +0.01-0.1mg/L LNAA +0.1-0.2mg/L paclobutrazol +20-30g/L sugar +7g/L agar, the pH value is 5.8-6.0, after proliferation culture for 4-6 weeks, proliferation is carried out for 3-4 times, and the plant height of the cluster buds is up to 1.5-2 cm;
(4) rooting culture: cutting the cluster seedlings obtained in the step (3) into 2-3 plants, inoculating the 2-3 plants into a rooting culture medium MS +0.05-0.1mg/L6-BA +0.5-1.0mg/L LNAA +0.1-0.2mg/L paclobutrazol +20-30g/L sugar +7g/L agar, controlling the pH value to be 5.8-6.0, and germinating after rooting culture for 4-6 weeks;
(5) hardening and transplanting seedlings: and (3) transplanting the rooted seedlings obtained in the step (4) to a greenhouse for acclimatization for 5-7d, gently taking out tissue culture bottle seedlings by using forceps, soaking the whole plants in a diluent of 800-1000 times of 50% carbendazim wettable powder for 10min, taking out, airing water, planting the seedlings in a matrix, shading the light by 75-85% and keeping the humidity by 60-80%, and gradually increasing the light and reducing the humidity after culturing for 10-14d to obtain the complete plants with exposed lenses.
2. The culture method for in vitro rapid propagation of bare lens according to claim 1, which is characterized in that: in the steps (2) - (4), the culture conditions are that the temperature is 20 +/-2 ℃, the illumination intensity is 3000-5000 lx, the illumination is 12h/d, and the relative humidity is 50% -60%.
3. The culture method for in vitro rapid propagation of bare lens according to claim 1, which is characterized in that: and (5) lightly taking out the tissue culture bottle seedlings by using tweezers, and shearing off the roots of the rooted seedlings by using sterilized scissors.
4. The culture method for in vitro rapid propagation of bare lens according to claim 1, which is characterized in that: in the step (5), the matrix comprises vermiculite, humus soil and perlite in a ratio of 1:1: 1.
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Non-Patent Citations (4)
| Title |
|---|
| Response of Pilea Plant Pilea cadieri for Micropropagation using Tissue Culture Techniques;Layous L .;《Jordan Journal of Agricultural ences》;20131231;第9卷(第4期);第620页左栏第3段，第621页以及表1-4 * |
| 异株荨麻(Urtica dioica L.)研究及开发利用进展;朱涛涛等;《作物杂志》;20151231(第05期);第18-24页 * |
| 粗齿冷水花的组织培养;蒋泽平等;《植物生理学通讯》;19911231(第01期);摘要以及第42页左栏第3段 * |
| 苎麻遗传转化再生体系的建立;孔华等;《分子植物育种》;20061231;第4卷(第02期);第233-237页 * |
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