CN108129551A - Peptide with skin whitening effects and application thereof - Google Patents
Peptide with skin whitening effects and application thereof Download PDFInfo
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- CN108129551A CN108129551A CN201710194162.7A CN201710194162A CN108129551A CN 108129551 A CN108129551 A CN 108129551A CN 201710194162 A CN201710194162 A CN 201710194162A CN 108129551 A CN108129551 A CN 108129551A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
- C07K7/04—Linear peptides containing only normal peptide links
- C07K7/06—Linear peptides containing only normal peptide links having 5 to 11 amino acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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- Mycology (AREA)
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- Cosmetics (AREA)
Abstract
The present invention relates to peptides with skin whitening effects and application thereof.Present invention is specifically related to a kind of Essences with skin whitening effects, and it includes following peptides：Val‑Lys‑Ser‑Arg‑Glu‑Arg‑Asp‑Gln(VKSRERDQ)(SEQ ID NO:And/or His Gly His Pro Asp Gln Ala Pro Phe (HGHPDQAPF) (SEQ ID NO 1):2).The Essence of the present invention also includes following component：Glutathione, 1,3 propylene glycol, rose extract, glycyrrhiza glabra extract, mulberry-leaf extract, dried tremella extract, glucan, Sodium Hyaluronate, aloe extract, plant polyose and lauroyl arginine ethyl ester.The Essence of the present invention has apparent whitening effect.
Description
Technical field
The present invention relates to the Essence with skin whitening effects, the Essence includes the non-natural with white-skinned face function
Peptide.The invention further relates to application of the non-natural peptide in cosmetics or personal care composition.
Background of invention
In cosmetic field, a variety of natural and synthetic peptides are all extensively with wherein.In general, it is to utilize it that peptide is included in cosmetics
Functional activity, such as the inhibition to enzyme, antiviral and antibacterium isoreactivity.
For example, United States Patent (USP) 6,777,389 describes make-up composition, it includes each sections with endogenous elastin laminin
Corresponding or homologous peptide, including valine-Val-Pro-glutamine, val-ala-ala-arg-dried meat
Propylhomoserin-glycine, leucine-Gly-Ala-Gly-Gly-Ala-Gly and valine-glycine-
Valine-Hyp-Gly and they enhance by with elastin laminin deficiency in relation to the problem of skin appearance
Using.
US6,372,717 disclose make-up composition, it includes the synthetic lipophilic derivative of Tyr-Arg peptides, including N-
Acetyl group-L-Tyr-L-Arg-O- cetyls stimulate, mild pain, hot, cold effect for alleviating, friction on skin or
The feeling of mechanical sexual assault.
US6,245,342 discloses the makeup of the peptide comprising the His-Phe-Arg-Trp from α-MSH (melanotropin)
The application of composition.It is said that the make-up composition has the generation of stimulation melanogen and antiinflammatory property.
US6,358,929, which describes the peptide comprising sequence 11-13 ALPHA-MSH, to be prevented or is subtracting as additive
Application in the few make-up composition for not tolerating reaction related with contact hypersensitivity.
US6,235,291 describes peptide (TyrD-Phe Phe D-His Leu-Met NH2) and peptide (D-NicLys Pro
3-Pal Pro D-Cl2 Phe Asn D-TrpPhe D-Trp Leu Nle NH2) it is sensitive in treatment as Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 antagonist
Application in the cosmetics of property skin.
US6,235,291 is related to peptide (the Gly Ile Gly Asp Pro to antibacterium, mould and/or virus activity
Val Thr Cys Leu Lys Ser Gly Ala Ile Cys His Pro Val Phe Cys Pro Arg Arg Tyr
Lys Gln Ile Gly Thr Cys Gly Leu Pro Gly Thr Thr Cys Cys Lys Lys Pro) application and
Its application in make-up composition.
A variety of beauty means can enable the colour of skin more whitening or uniform hue.A kind of common means are by sun-proof opacifier or prevent
Interleaving agent is shone to be introduced into cosmetic composition.Sun-proof opacifier is usually organic compound, their effect is specified via absorbing
The solar ultraviolet radiation of wave-length coverage is so as to make the ultraviolet radioactive that can not reach skin surface.Ultraviolet radioactive is believed to be pigmenting of skin
Or the reason of tanned, if tanned uneven, consumer does not like this tanned.Sun-proof interleaving agent is usually inorganic compound,
They function as physical barriers to prevent various solar radiations (not only including ultraviolet light but also including visible ray).
Another common means are that skin-whitening agents are introduced into cosmetic composition.Skin-whitening agents be usually molecule or
Composition, they change the melanocyte realized in skin via the conversion of skin mesophytization and are formed, and thus change the color and table of skin
It sees.In human skin, polymerization of the formation originating from l-tyrosine this monoamino-acid of melanocyte pigment, by way of one group of enzymatic
With spontaneous chemical reaction.The effect of this existing tyrosinase more particularly in the melanosome compartment of melanocyte.It is black
Plain corpusculum content is transferred to multiple peripheral keratinocytes from the melanocyte.Whole process is influenced by a variety of hormones, at this
Melanotropin (MSH) is adequately studied in a little hormones.
For example, JP2001002527 (Lion) discloses preparation for external application to skin, including being based on composition gross weight
The cell adhesion for melanocyte of 0.000001-30wt% resets agent, such as including arginine-glycine-aspartic acid
Peptide including sour (RGD) sequence, including Tyrosine-Isoleucine-glycine-serine-arginine, this amino acid sequence exists
Interior peptide, 3- denitrogenations adenosine, carboxamides derivatives, inositol polyamines, salicylic acid and triterpenic acid derivative, lactose etc..
In addition, United States Patent (USP) 5,126,327 discloses melanotropin inhibitor, there is following formula amino acid sequence in the molecule
Row：- His-Ser-Arg-Trp- ,-Trp-Arg-Ser-His- ,-Leu-Ala-Cys-Ala-Arg-, wherein His, Ser, Arg,
Trp, Leu, Ala and Cys represent L- or D-His, serine, arginine, tryptophan, leucine, alanine and half Guang respectively
Histidine residue.Preparation for external application to skin it discloses melanotropin inhibitor and containing the inhibitor, the inhibitor prevent or control
It treats the excessive melanocyte caused by melanocyte function enhances and generates caused chloasma and freckle symptom.
Invention summary
To develop the new type of peptides with skin whitening effects, present inventor has performed insistent research and peptide has been screened
Library.Finally, the inventor has discovered that the new type of peptides with excellent skin whitening efficacy, finally achieves the object of the present invention.
The present invention provides a kind of Essences with whitening effect, and it includes SEQ ID NO：1 peptide, wherein the peptide
Sequence it is as follows：Val-Lys-Ser-Arg-Glu-Arg-Asp-Gln(VKSRERDQ)(SEQ ID NO:1).
The present invention also provides a kind of Essences with whitening effect, and it includes SEQ ID NO：1 peptide, wherein described
The sequence of peptide is as follows：His-Gly-His-Pro-Asp-Gln-Ala-Pro-Phe(HGHPDQAPF)(SEQ ID NO:2).
The present invention also provides a kind of Essences with whitening effect, and it includes SEQ ID NO：1 peptide and SEQ ID
NO：2 peptide.
Preferably, Essence of the invention also includes following component：Glutathione, 1,3-PD, rose extract,
Glycyrrhiza glabra extract, mulberry-leaf extract, dried tremella extract, glucan, Sodium Hyaluronate, aloe extract, plant polyose and
Lauroyl arginine ethyl ester.
Preferably, Essence of the invention includes SEQ ID NO：1 peptide, glutathione, 1,3-PD, rose extraction
Object, glycyrrhiza glabra extract, mulberry-leaf extract, dried tremella extract, glucan, Sodium Hyaluronate, aloe extract, plant polyose
With lauroyl arginine ethyl ester.
Preferably Essence of the invention includes SEQ ID NO：2 peptide, glutathione, 1,3-PD, rose extraction
Object, glycyrrhiza glabra extract, mulberry-leaf extract, dried tremella extract, glucan, Sodium Hyaluronate, aloe extract, plant polyose
With lauroyl arginine ethyl ester.
Preferably Essence of the invention includes SEQ ID NO：1 and SEQ ID NO：2 peptide combination, glutathione, 1,
3-propanediol, rose extract, glycyrrhiza glabra extract, mulberry-leaf extract, dried tremella extract, glucan, Sodium Hyaluronate, reed
Luxuriant growth extract, plant polyose and lauroyl arginine ethyl ester.
Preferably, in the above-mentioned Essence of the present invention, in terms of weight %, SEQ ID NO：1 and SEQ ID NO：2 peptide
Content respectively in 0.0005-0.001, glutathione 2.0-4.0,1,3-PD 4.0-6.0, rose extract 0.5-2.5,
Glycyrrhiza glabra extract 2.0-4.0, mulberry-leaf extract 3.0-6.0, dried tremella extract 2.0-6.0, glucan 3.0-7.0 are transparent
Matter acid sodium 1.5-3.5, aloe extract 2.0-4.0, plant polyose 0.4-0.8, lauroyl arginine ethyl ester 0.3-0.6.
Preferably, in Essence of the invention, in terms of weight %, SEQ ID NO：1 and SEQ ID NO：The content of 2 peptide
Respectively 0.0005, glutathione 3.0,1,3-PD 5.0, rose extract 1.5, glycyrrhiza glabra extract 3.0, mulberry leaf carry
Take object 5.0, dried tremella extract 4.0, glucan 5.0, Sodium Hyaluronate 2.5, aloe extract 3.0, plant polyose 0.6, bay
Acyl arginine ethyl ester 0.5.
One object of the present invention is to provide the peptide with skin whitening effects.
Another object of the present invention is provides a kind of composition for whitening skin.
A further object of the present invention is to provide a kind of method with skin-whitening.
Purpose to realize the present invention, the first aspect of the present invention provide peptide, are selected from following peptide：
(i) peptide is formed it includes or by following amino acid residue：
Val-Lys-Ser-Arg-Glu-Arg-Asp-Gln(VKSRERDQ)(SEQ ID NO:1)；Or
(ii) peptide is formed it includes or by following amino in sour residue：
His-Gly-His-Pro-Asp-Gln-Ala-Pro-Phe(HGHPDQAPF)(SEQ ID NO:2)。
Preferred aspect according to the present invention, provides the combination of peptide, and it includes SEQ ID NO:1 and SEQ ID NO:2
Peptide combination.
The present invention provides skin lightening compositions, and it includes the SEQ ID NO of the present invention:1 peptide and cosmetically may be used
The carrier of receiving.Preferably, the lightening compositions are the forms of Essence.
Preferred aspect according to the present invention, provides skin lightening compositions, and it includes the SEQ ID NO of the present invention:1
With SEQ ID NO：2 peptide combination and cosmetically acceptable carrier.
In addition, the SEQ ID NO the present invention provides the present invention:1 peptide is used for the purposes of skin-whitening.
According to a preferred aspect of the present invention, the SEQ ID NO of the present invention are provided:1 and SEQ ID NO：2 peptide combination is used
In the purposes of skin-whitening.
In the detailed description for having read the following present invention, after illustrative embodiment and embodiment, this hair
Bright these and other aspects will become obvious those skilled in the art.
Detailed description
Two peptides are claimed in the present invention, that is, with SEQ ID NO:1 peptide (i) has SEQ ID NO:2
Peptide (ii).The two new peptides are combined with α-MSH (α-melanotropin) so that α-MSH is inhibited to be attached on MC1R.When these peptides
During for skin lightening compositions, which provides the benefit in terms of skin-whitening.The two peptides are through using phage display
(PD) technology identifies, and phage display (PD) technology is differentiating specifically binding target molecule in biotechnology
The method of peptide.From extremely more possibility peptides, four unique peptides are identified first as potential α-MSH conjugates.From this
It in four peptides, finds there is extremely strong associativity for α-MSH conjugates there are two peptide, selects the two peptides for function benefit
Research.Then the effect of these peptides reduce melanin content that is, potential are tested in melanin content experiment.
Not only confirm that each self energy of the two peptides reduces melanin content, but also be also noted that they are used together when and cooperate with
Interaction.
As used herein, it is intended to that there are them in the general of this field unless otherwise specifically defined all terms
Meaning.Term " amino acid " is intended to include naturally occurring amino acid and non-naturally occurring amino acid, broadly including institute
Having tool, there are one carboxyls and the small molecule of at least one primary or secondary amine groups that can form peptide bond.Term " peptide " is intended to include all tools
There is the molecule of at least one peptide bond, therefore including dipeptides, tripeptides, oligopeptides and the polypeptide for being up to about 20 amino acid residues.
Term " peptide " also include it is with one or more linkers, interval base or terminal groups, be not the structure of amino acid.
The peptide of the present invention provides in cosmetically acceptable carrier.Carrier can be hydrophobic or hydrophilic.It is suitable
When hydrophobic carrier include for example, commonly using waxy nonionic, such as the ester and ether of fatty alcohol and aliphatic acid, carbon in cosmetics
Chain length is C4 to C22, most preferably preferably C8 to C18, C12 to C18.
The example of fatty hydrophobic carrier includes isopropyl myristate, isopropyl palmitate, octyl palmitate, lanolin fatty acid
Isopropyl ester, acetulan, the benzoic ether of C12-C15 alcohol, Octanoic acid, hexadecyl ester, cetin, myristic acid meat
Cardamom ester, Tetradecyl lactate, acetic acid cetyl, two caprylic/capric propylene glycol esters, decyl oleate, acetylated lanolin, enanthic acid
Stearate, malic acid diisopstearate, octyl hydroxystearate, octyl hydroxystearate, isopropyl isostearate etc..
Appropriate hydrophilic support solution can be, such as common alkoxylated glycol in glycol and cosmetics, including
Ethylene glycol, diethylene glycol, triethylene glycol, propylene glycol, dipropylene glycol etc..
Skin lightening compositions can be configured to Essence, nutrient solution, nourishing cream, emulsifiable paste, toner, slurries, spray,
Stylus and other forms well known by persons skilled in the art.Essence and nutrient solution are currently preferred product forms.Most preferably
Ground, skin lightening compositions of the invention are formulated into the form of nutrient solution.
In cosmetically acceptable carrier, the concentration range of peptide can be 1 μM to 200 μM, preferably 10ppb to 150 μM,
More preferable 20 μM to 100 μM, most preferably 36 μM to 50 μM.
Skin lightening compositions typically comprise above-mentioned carrier solution, and level is about 0.01% to about 90% weight,
Preferably from about 0.1% to about 50% weight, more preferably from about 0.1% to about 20% weight, most preferably from about 1% to about 10% weight.
As it is used herein, "comprising" and " comprising ", " containing " or " being characterized in that " it is synonymous, and in being included in
Or open, and it is not excluded for the other element do not stated or method and step.Term "comprising" any table herein
State, particularly in the method for the description present invention, purposes or during product, it is thus understood that including substantially by the component or element or
Those products, method and the purposes that step is formed and is made of the component or element or step.The sheet of description exemplified here
Invention suitably can be there is no the situations of any one or more of element not specifically disclosed herein, one or more limitations
Under put into practice.
The present invention skin-whitening product included in be related to the skin-whitening product specification can contain it is as follows
Content：Effect with effect (such as skin-whitening), method of administration (such as being uniformly applied to skin, such as face and neck) and
Issuable side effect etc..
The term and statement used herein is used as descriptively rather than restrictive term, and in such term and statement
Use in it is not expected exclude shown in and the feature or part thereof any equivalent, it is appreciated that various modifications are being asked
It is possible in the scope of the present invention of protection.It is therefore understood that although the present invention by preferred embodiment and optionally
Feature specifically discloses, but the modification and transformation of concept disclosed herein, and such modification may be used in those skilled in the art
It is considered as in the scope of the present invention such as defined by accessory claim with variation.
It to be illustrated more clearly that the present invention, is described in detail in conjunction with following examples, but these embodiments are only
To the exemplary description of the present invention, the limitation to the application should not be construed as.
Embodiment
The following examples are for illustrating the present invention in a non limiting manner.
Embodiment 1：The analysis of the skin whitening effects of peptide of the present invention
With the SEQ ID NO of various concentration：1 or SEQ ID NO：2 peptide processing cell, then measures and presses down in melanogenesis
Activity in system.The 5%CO at 37 DEG C2Mouse melanocytes are cultivated in atmosphere in the DMEM for being supplemented with 10%FBS.By cell
It is seeded in 24 orifice plate (1X105Cells/well) each hole in and confirm its attachment.Cell is cultivated with each test group 3 days：(a)
Solvent is only used, as negative control；(b) with 20 μ g/ml α-MSH, as positive control；(c) add 1 with 20 μ g/ml α-MSH, 10,
100 and 150 μ g/ml peptides.After centrifugation, it removes culture medium and visually observes the level of melanogenesis.
The result shows that peptide has inhibition with concentrationdependent manner to melanogenesis.Compared to α-MSH processing groups,
The color of culture medium in the peptide processing group of the present invention becomes transparent, and observe cell number little change or unchanged, table
The bright inhibition occurred to melanogenesis.
In order to analyze the burst size of melanocyte, the absorbance value of culture medium is measured under 490nm.The result shows that compared to the moon
Property control group and positive controls, the burst size of melanocyte significantly reduces in peptide processing group of the invention.These results indicate that not
Whether pipe melanogenesis incitant is acted on skin, and peptide of the invention can be by inhibiting melanogenesis and brilliant white skin
Color.
Embodiment 2：Synergistic effect
Include 1X105Mouse skin horn cell and 5X104The 2 of mouse skin melanocyte coloured deeply:1 ratio mixes
Object is inoculated in 24 porocyte culture plates per hole, to establish co-culture system.After being incubated overnight, cell is with including peptide of the present invention
Fresh culture processing, incubates 72 hours after processing.Then culture medium is removed, replaces with 80 μ l dimethyl sulfoxide (DMSO)s (DMSO).Training
Foster plate is further incubated 1 hour at 65 DEG C with DMSO, and grinding hole content, same sample is tested for melanin content later
(reading in plate reader in 450nm) and protein assess (BiCinchoninic Acid methods).Melanin content reduces percentage
Rate be relative to it is untreated in contrast.As a result it is shown in the following table 1.
Table 1
From the data of table 1 can be seen that two kinds of peptides of the present invention between cooperative interaction thus assign reinforcement skin it is beautiful
White effect.
Embodiment 3：The preparation of Essence
| Ingredient | Content (weight %) |
| Peptide (the SEQ ID NO of the present invention：1) | 0.001 |
| Glutathione | 3.0 |
| 1,3-PD | 5.0 |
| Rose extract | 1.5 |
| Glycyrrhiza glabra extract | 3.0 |
| Mulberry-leaf extract | 5.0 |
| Dried tremella extract | 4.0 |
| Glucan | 5.0 |
| Sodium Hyaluronate | 2.5 |
| Aloe extract | 3.0 |
| Plant polyose | 0.6 |
| Lauroyl arginine ethyl ester | 0.5 |
| Purified water | Residual volume |
| It amounts to | 100 |
Glucan is dissolved in purified water and adds in 1,3-PD, stirs and adjusts after being stirring evenly and then adding into other components
Essence is made.
Embodiment 4：The preparation of Essence
Glucan is dissolved in purified water and adds in 1,3-PD, stirs and adjusts after being stirring evenly and then adding into other components
Essence is made.
Embodiment 5：The preparation of Essence
Glucan is dissolved in purified water and adds in 1,3-PD, stirs and adjusts after being stirring evenly and then adding into other components
Essence is made.
Embodiment 6
Glucan is dissolved in purified water and adds in 1,3-PD, stirs and adjusts after being stirring evenly and then adding into other components
Essence is made.
Embodiment 7
| Ingredient | Content (weight %) |
| Peptide (the SEQ ID NO of the present invention：2) | 0.001 |
| Glutathione | 3.0 |
| 1,3-PD | 5.0 |
| Rose extract | 1.5 |
| Glycyrrhiza glabra extract | 3.0 |
| Mulberry-leaf extract | 5.0 |
| Dried tremella extract | 4.0 |
| Glucan | 5.0 |
| Sodium Hyaluronate | 2.5 |
| Aloe extract | 3.0 |
| Plant polyose | 0.6 |
| Lauroyl arginine ethyl ester | 0.5 |
| Purified water | Residual volume |
| It amounts to | 100 |
Glucan is dissolved in purified water and adds in 1,3-PD, stirs and adjusts after being stirring evenly and then adding into other components
Essence is made.
Embodiment 8
| Ingredient | Content (weight %) |
| Peptide (the SEQ ID NO of the present invention：1 and 2) | Equivalent, each 0.0005 |
| Glutathione | 3.0 |
| 1,3-PD | 5.0 |
| Rose extract | 1.5 |
| Glycyrrhiza glabra extract | 3.0 |
| Mulberry-leaf extract | 5.0 |
| Dried tremella extract | 4.0 |
| Glucan | 5.0 |
| Sodium Hyaluronate | 2.5 |
| Aloe extract | 3.0 |
| Plant polyose | 0.6 |
| Lauroyl arginine ethyl ester | 0.5 |
| Purified water | Residual volume |
| It amounts to | 100 |
Glucan is dissolved in purified water and adds in 1,3-PD, stirs and adjusts after being stirring evenly and then adding into other components
Essence is made.
Embodiment 9
According to the formula shown in embodiment 3,4,5,6,7 and 8, the composition for external application of Essence form, and city are modulated
Purchase cleawhite over-night essences embodiment as a comparison.For each composition for external application, the women of 10 30-35 Sui is allowed respectively in face
Use feeling during painting preparation is evaluated according to following standards.The side of wherein every subject's face is Test sites, and opposite side is
Compare position.
5 points：Skin surface is smooth, compacts, and whitening effect protrudes；
4 points：Skin surface is smooth, and whitening effect is visible；
3 points：There is no visible variation；
2 points：Skin surface slightly has rough feeling, without whitening effect；
1 point：Skin surface has rough feeling, without whitening effect.
Calculate the average mark of above-mentioned evaluation result, the results showed that, the average mark of embodiment 3,4,6,7 and 8 4.4 points with
On, control sample is equally divided into 3.0 points.The effect of embodiment 8 is most prominent, is equally divided into 4.8.Pass through above-mentioned preferred reality
Applying the description of scheme, the present invention is described it should be appreciated that technical staff can do these embodiments
Go out change and change, without departing from the spirit or scope of the present invention as expressed by following claims.
Claims (10)
1. the Essence with whitening effect, it includes SEQ ID NO：1 and/or SEQ ID NO：2 peptide.
2. Essence according to claim 1, also sweet comprising glutathione, 1,3-PD, rose extract, light fruit
Careless extract, mulberry-leaf extract, dried tremella extract, glucan, Sodium Hyaluronate, aloe extract, plant polyose and lauroyl
Arginine ethyl ester.
3. Essence according to claim 1 or 2, wherein in terms of weight %, SEQ ID NO：1 and SEQ ID NO：2
The content of peptide is respectively in 0.0005-0.001, glutathione 2.0-4.0,1,3-PD 4.0-6.0, rose extract 0.5-
2.5, glycyrrhiza glabra extract 2.0-4.0, mulberry-leaf extract 3.0-6.0, dried tremella extract 2.0-6.0, glucan 3.0-7.0,
Sodium Hyaluronate 1.5-3.5, aloe extract 2.0-4.0, plant polyose 0.4-0.8, and lauroyl arginine ethyl ester 0.3-
0.6。
4. Essence according to claim 3, wherein in terms of weight %, SEQ ID NO：1 and SEQ ID NO：2 peptide
Content is respectively 0.0005, glutathione 3.0,1,3-PD 5.0, rose extract 1.5, glycyrrhiza glabra extract 3.0, mulberry
Leaf extract 5.0, dried tremella extract 4.0, glucan 5.0, Sodium Hyaluronate 2.5, aloe extract 3.0, plant polyose 0.6,
Lauroyl arginine ethyl ester 0.5.
5. peptide, with SEQ ID NO：2 amino acid sequence.
6. peptide according to claim 5, by SEQ ID NO：2 amino acid sequence composition.
7. the combination of peptide, it includes SEQ ID NO：1 peptide and SEQ ID NO：2 peptide.
8.SEQ ID NO：1 peptide and/or SEQ ID NO：Purposes of 2 peptide in composition for whitening skin is prepared.
9. purposes according to claim 8, wherein the composition is the form of Essence.
10. purposes according to claim 9, wherein the composition for whitening skin is packaged as commercially available beauty
Product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710194162.7A CN108129551B (en) | 2017-03-28 | 2017-03-28 | Peptide having skin whitening effect and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710194162.7A CN108129551B (en) | 2017-03-28 | 2017-03-28 | Peptide having skin whitening effect and use thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108129551A true CN108129551A (en) | 2018-06-08 |
| CN108129551B CN108129551B (en) | 2020-03-31 |
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| CN201710194162.7A Active CN108129551B (en) | 2017-03-28 | 2017-03-28 | Peptide having skin whitening effect and use thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110354054A (en) * | 2019-08-29 | 2019-10-22 | 广州重生化妆品实业有限公司 | A kind of formula and preparation method thereof of natural skin maintenance Essence |
| CN113134042A (en) * | 2021-06-10 | 2021-07-20 | 台山市力丰投资有限公司 | Traditional Chinese medicine composition for treating chloasma and application thereof |
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| CN110354054A (en) * | 2019-08-29 | 2019-10-22 | 广州重生化妆品实业有限公司 | A kind of formula and preparation method thereof of natural skin maintenance Essence |
| CN113134042A (en) * | 2021-06-10 | 2021-07-20 | 台山市力丰投资有限公司 | Traditional Chinese medicine composition for treating chloasma and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108129551B (en) | 2020-03-31 |
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