CN108129420A - A kind of method of dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin - Google Patents
A kind of method of dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin Download PDFInfo
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- CN108129420A CN108129420A CN201611087455.7A CN201611087455A CN108129420A CN 108129420 A CN108129420 A CN 108129420A CN 201611087455 A CN201611087455 A CN 201611087455A CN 108129420 A CN108129420 A CN 108129420A
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- dimethyl ether
- extraction
- fucoxanthin
- phaeodactylum tricornutum
- ether fluid
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D303/00—Compounds containing three-membered rings having one oxygen atom as the only ring hetero atom
- C07D303/02—Compounds containing oxirane rings
- C07D303/12—Compounds containing oxirane rings with hydrocarbon radicals, substituted by singly or doubly bound oxygen atoms
- C07D303/32—Compounds containing oxirane rings with hydrocarbon radicals, substituted by singly or doubly bound oxygen atoms by aldehydo- or ketonic radicals
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D301/00—Preparation of oxiranes
- C07D301/32—Separation; Purification
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- Extraction Or Liquid Replacement (AREA)
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- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The invention discloses a kind of method of dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin, including step：1) the fresh algae freeze thawing broken wall of Phaeodactylum tricornutum；2) dimethyl ether fluid dynamic gradient counter-current extraction broken wall algal gel；3) extract liquor precipitation isolates fucoxanthin；It can effectively avoid destruction of the physical chemistry means dehydration to active ingredient in microalgae, and extraction process energy consumption is greatly reduced, dynamic gradient counter-current extraction operation, the concentration of extract liquor active ingredient can be effectively improved, reduce Solvent quantity, extraction efficiency is improved, realizes high efficiency extraction Phaeodactylum tricornutum fucoxanthin, there is reliable safety.
Description
Technical field
The invention belongs to both culturing microalgae technical fields, and in particular to a kind of dimethyl ether fluid extraction Phaeodactylum tricornutum rock algae is yellow
The method of element.
Background technology
Fucoxanthin, also known as pheophytin, fucoxanthine, molecular formula C42H58O6, structural formula such as following formula, molecular weight 658.91,
Density 1.09,166~168 DEG C of fusing point, CAS accession number 3351-86-8 belong to allene type carotenoid, are brown alga, diatom, gold
A kind of important carotenoid contained by algae and yellowish green algae is that the substance of brown and the finger of Diatomeae plant is presented in brown alga
Mark property pigment.
Fucoxanthin unique structure shows the potential value advantage with application prospect in terms of a variety of pharmacological activity,
Wherein anti-inflammatory, antitumor, anti-fat, anti-oxidant, anti-acne, anti-diabetic, anti-malarial and the physiological activity such as anti-lipid are by part
It confirms, other potential physiological activity are carried out in positive research and exploration by scientist.
At present, the raw material available for extracting fucoxanthin has very much, such as brown alga, diatom algae.Phaeodactylum tricornutum is also
The typical unicellular diatom of one of which, and Phaeodactylum tricornutum have be easy to cultivate, can grow throughout the year, the speed of growth soon and is easy to
The characteristics of regulation and control, cultivation Phaeodactylum tricornutum production fucoxanthin have the advantage of bigger.
The conventional method of Phaeodactylum tricornutum fucoxanthin extraction is organic solvent extraction, and this method is simple and practicable, more often
Organic solvent includes methanol, ethyl alcohol, acetone, ether, petroleum ether and dimethyl sulfoxide (DMSO) etc., they can be used alone or by one
Certainty ratio is used in mixed way.However, Extracting temperature is higher in organic solvent extraction, and fucoxanthin loss, product dissolvent residual, peace
Full hidden danger etc., is respectively provided with apparent limitation.
Many researchers are dedicated to exploring novel extractive technique, such as supercritical extraction technique, pressurized liquid extraction method, microwave
Extraction, ultrasonic extraction etc..These Novel extraction methods have a variety of advantages, can improve extraction efficiency, when shortening extraction
Between, extraction funds are saved, and reduce the harm to environment.However, the use of microwave, ultrasonic wave, a kind of only supplementary means,
Needing to be combined with other abstraction techniques can just play a role.Supercritical extraction technique is also ground in the extraction of fucoxanthin
Study carefully, but supercritical solvent CO2Extraction processing must can be carried out in the superelevation pressure condition of more than 25MPa, greatly restrict it
Industrial applications.Pressurized liquid extraction method is under higher temperature and pressure, with organic solvent extraction bioactive substance
A kind of quickly and effectively extracting method, this method considerably reduce the dosage of organic solvent, but fail to change organic solvent extraction
The technological deficiency followed the example of.
Subcritical extraction method is a kind of novel extraction and separation technology to grow up about ten years recently.Subcritical abstraction side
Method be by the use of subcritical fluids as extractant, closed, anaerobic, low pressure pressure vessel in, according to organic matter similar compatibility
Principle, spread by extracting the molecule of material and extractant in soaking process, reach the liposoluble constituent in solid material
It is transferred in the extractant of liquid, then is detached extractant with purpose product by the process of reduction vaporization, finally obtain purpose
A kind of novel extraction and isolation technics of product.Subcritical fluid extraction has many advantages compared to other separation methods：Nontoxic, nothing
Harmful, pollution-free, non-thermal technology, the biologically active prod for retaining extract is not destroyed, is not aoxidized, production capacity is big, can industrialize extensive life
Production, energy saving, operating cost is low, is easy to detach with product.
The method that patent CN 101077991A disclose extracting natural pyrethrin by subcritical dimethyl ether fluid.Patent CN
10297006A discloses the chrysanthemum absolute oil of subcritical fluids low temperature process extraction.Patent CN104738251A discloses a kind of Asia
The method of supercritical fluid extraction tea extract.It is green that patent CN105400696A discloses a kind of subcritical dimethyl ether fluid extraction
The method of xanthoxylum oleoresin.This four parts of patents make extractant using subcritical fluids dimethyl ether, and process is similar, only
Processing material differs, but be not directed to the extraction of Phaeodactylum tricornutum fucoxanthin.
In the extraction production practices of Phaeodactylum tricornutum fucoxanthin, the fresh algae of Phaeodactylum tricornutum aqueous usually more than 60% uses
A variety of drying and dehydrating methods such as hot-working, electromagnetic processing, vacuum processing cause Phaeodactylum tricornutum fucoxanthin larger break
It is bad；The siliceous shell combination of Phaeodactylum tricornutum is more close, and organic solvent is difficult to effectively to infiltrate through in cytoplasm and the fat such as pigment
Plastid contacts, and fucoxanthin extraction efficiency is low；Phaeodactylum tricornutum fucoxanthin content about 1.0%, extract liquor concentration is at a fairly low,
Extraction solvent remains high with energy consumption.General subcritical extraction method is also difficult to meet the requirements using static immersing extraction.
Because of material characteristic uniqueness, active constituent property is active, and necessarily the extraction of Phaeodactylum tricornutum fucoxanthin is proposed more
High requirement.
Invention content
Needle of the present invention is directed to defective workmanship and deficiency in existing Phaeodactylum tricornutum fucoxanthin extractive technique, proposes a kind of
The method of low temperature, efficient, safety dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin.
In order to solve the above technical problems, the technical scheme is that：
A kind of method of dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin, includes step：
1) the fresh algae freeze thawing broken wall of Phaeodactylum tricornutum；
2) dimethyl ether fluid dynamic gradient counter-current extraction broken wall algal gel；
3) extract liquor precipitation isolates fucoxanthin.
As preferred technical solution, the fresh algae of step 1) intermediate cam brown algae is Phaeodactylum tricornutum through supercentrifuge
The fresh algae of drying, aqueous mass percent are 65.0%~75.0%.
As preferred technical solution, the fresh algae of Phaeodactylum tricornutum is first quick-frozen, then refrigerates, and finally thaws and realizes freeze thawing
Broken wall, quick freezing temperature are -70 DEG C~-40 DEG C, and the quick-frozen time is 8.0h~12.0h, and -20 DEG C~-10 DEG C of refrigerated storage temperature solves
It is 20 DEG C~30 DEG C to freeze temperature, and thawing time is 0.5~1.5h, and number of freezing and thawing is 1~3 time.
As preferred technical solution, in the step 2) dimethyl ether fluid be subcritical fluids, pressure for 0.5MPa~
1.0MPa, temperature are 40 DEG C~60 DEG C.
As preferred technical solution, the residence time of dimethyl ether fluid is 1.0~2.0 hours in the step 2)
Operation is extracted as preferred technical solution, in the step 2) as dynamic gradient counter-current extraction, dimethyl ether fluid
One group of extractor is continued through successively, and dimethyl ether con steps up in single tank, and the front end list tank for completing extraction operation is detached from extraction
System is taken, the new single tank for loading broken wall algal gel is connected into the end of extracting system, dimethyl ether fluid and broken wall algal gel reverse flow.
As preferred technical solution, the extractor group is made of 4~6 one-pot tandems.
As preferred technical solution, the pressure of extract liquor precipitation is 0.05MPa~0.10MPa in the step 3), warm
Spend is 50 DEG C~70 DEG C.
As preferred technical solution, output fucoxanthin after thick fucoxanthin grease centrifuges after the precipitation, from
Scheming rotating speed is 4500 revs/min~10000 revs/min.
It is using advantageous effect caused by above-mentioned technical proposal：
1) wet algal gel uses mechanical dehydration, is directly entered system of processing after removing appearance water, can effectively avoid physical chemistry
Means are dehydrated the destruction to active ingredient in microalgae, and extraction process energy consumption is greatly reduced.
2) freeze thawing broken wall and fresh algae processing of harvesting and storing brings out the best in each other, on the one hand, due to the haline water of fresh algae entrainment have it is strong
Permeability, into intracellular brine formed ice crystal, expansion damage cell wall, on the other hand, fresh algae need to pass through freezing, reduce water
Activity, collection storage.
3) dimethyl ether is water-soluble, and solubility is up to 30g in every 100g water at room temperature, is greatly promoted dimethyl ether fluid
It is intracellular to penetrate into Phaeodactylum tricornutum, extracts the pigments such as fat-soluble fucoxanthin and lipid, and dimethyl ether fluid is in subcritical shape
State, extraction temperature is low, and Phaeodactylum tricornutum active ingredient is not destroyed substantially.
4) dynamic gradient counter-current extraction operation can effectively improve the concentration of extract liquor active ingredient, reduce Solvent quantity,
Extraction efficiency is improved, realizes high efficiency extraction Phaeodactylum tricornutum fucoxanthin.
5) it is gas under dimethyl ether normal temperature and pressure, can be removed from fucoxanthin thoroughly, and extraction process is always in low
Under temperature control system, essentially a physical dissolution, evaporation process, fucoxanthin keep its natural feature, have reliable safety.
By adopting the above-described technical solution, a kind of method of dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin, packet
Include step：1) the fresh algae freeze thawing broken wall of Phaeodactylum tricornutum；2) dimethyl ether fluid dynamic gradient counter-current extraction broken wall algal gel；3) extract liquor
Precipitation isolates fucoxanthin；Destruction of the physical chemistry means dehydration to active ingredient in microalgae is can effectively avoid, and is substantially dropped
Low extraction process energy consumption, dynamic gradient counter-current extraction operation can effectively improve the concentration of extract liquor active ingredient, reduce extractant
Dosage improves extraction efficiency, realizes high efficiency extraction Phaeodactylum tricornutum fucoxanthin, has reliable safety.
Description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it should be apparent that, the accompanying drawings in the following description is only this
Some embodiments of invention, for those of ordinary skill in the art, without having to pay creative labor, may be used also
To obtain other attached drawings according to these attached drawings.
Fig. 1 is Phaeodactylum tricornutum freeze thawing broken cells state vs figure of the present invention；
Fig. 2 is fucoxanthin high-efficient liquid phase chromatogram of the present invention.
Specific embodiment
A kind of method of dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin, includes step：
1) the fresh algae freeze thawing broken wall of Phaeodactylum tricornutum；
2) dimethyl ether fluid dynamic gradient counter-current extraction broken wall algal gel；
3) extract liquor precipitation isolates fucoxanthin.
The fresh algae of step 1) intermediate cam brown algae is the fresh algae that Phaeodactylum tricornutum is dried through supercentrifuge, containing water quality
Percentage is 65.0%~75.0%.
The fresh algae of Phaeodactylum tricornutum is first quick-frozen, then refrigerates, and finally thaws and realizes freeze thawing broken wall, and quick freezing temperature is -70
DEG C~-40 DEG C, the quick-frozen time is 8.0h~12.0h, and -20 DEG C~-10 DEG C of refrigerated storage temperature, thaw point is 20 DEG C~30 DEG C,
Thawing time is 0.5~1.5h, and number of freezing and thawing is 1~3 time.
In the step 2) dimethyl ether fluid be subcritical fluids, pressure be 0.5MPa~1.0MPa, temperature for 40 DEG C~
60℃。
The residence time of dimethyl ether fluid is 1.0~2.0 hours in the step 2)
Extraction operation is dynamic gradient counter-current extraction in the step 2), and dimethyl ether fluid continues through one group of extraction successively
Tank, dimethyl ether con steps up in single tank, and the front end list tank for completing extraction operation is detached from extracting system, new to load broken wall algal gel
Single tank be connected into the end of extracting system, dimethyl ether fluid and broken wall algal gel reverse flow.
The extractor group is made of 4~6 one-pot tandems.
The pressure of extract liquor precipitation is 0.05MPa~0.10MPa in the step 3), and temperature is 50 DEG C~70 DEG C.
Output fucoxanthin after thick fucoxanthin grease centrifuges after the precipitation, centrifuge speed are 4500 revs/min
Clock~10000 rev/min.
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without creative efforts
Embodiment shall fall within the protection scope of the present invention.
Embodiment one：
A kind of method of dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin, includes step：
1) the fresh algae freeze thawing broken wall of Phaeodactylum tricornutum；
2) dimethyl ether fluid dynamic gradient counter-current extraction broken wall algal gel；
3) extract liquor precipitation isolates fucoxanthin.
The fresh algae of step 1) intermediate cam brown algae is the fresh algae that Phaeodactylum tricornutum is dried through supercentrifuge, containing water quality
Percentage is 65.0%.
The fresh algae of Phaeodactylum tricornutum is first quick-frozen, then refrigerates, and finally thaws and realizes freeze thawing broken wall, and quick freezing temperature is -70
DEG C, the quick-frozen time is 8.0h, and -20 DEG C of refrigerated storage temperature, thaw point is 20 DEG C, and thawing time 0.5, number of freezing and thawing is 1 time.
Dimethyl ether fluid is subcritical fluids in the step 2), and pressure 0.5MPa, temperature is 40 DEG C.
The residence time of dimethyl ether fluid is 1.0 hours in the step 2)
Extraction operation is dynamic gradient counter-current extraction in the step 2), and dimethyl ether fluid continues through one group of extraction successively
Tank, dimethyl ether con steps up in single tank, and the front end list tank for completing extraction operation is detached from extracting system, new to load broken wall algal gel
Single tank be connected into the end of extracting system, dimethyl ether fluid and broken wall algal gel reverse flow.
The extractor group is made of 4 one-pot tandems.
The pressure of extract liquor precipitation is 0.05MPa in the step 3), and temperature is 50 DEG C.
Output fucoxanthin after thick fucoxanthin grease centrifuges after the precipitation, centrifuge speed are 4500 revs/min
Clock.
Embodiment two：
A kind of method of dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin, includes step：
1) the fresh algae freeze thawing broken wall of Phaeodactylum tricornutum；
2) dimethyl ether fluid dynamic gradient counter-current extraction broken wall algal gel；
3) extract liquor precipitation isolates fucoxanthin.
The fresh algae of step 1) intermediate cam brown algae is the fresh algae that Phaeodactylum tricornutum is dried through supercentrifuge, containing water quality
Percentage is 70.0%.
The fresh algae of Phaeodactylum tricornutum is first quick-frozen, then refrigerates, and finally thaws and realizes freeze thawing broken wall, and quick freezing temperature is -55
DEG C, the quick-frozen time is 102.0h, and -15 DEG C of refrigerated storage temperature, thaw point is 25 DEG C, thawing time 1.0h, number of freezing and thawing 23
It is secondary.
Dimethyl ether fluid is subcritical fluids in the step 2), and pressure 0.8MPa, temperature is 50 DEG C.
The residence time of dimethyl ether fluid is 1.5 hours in the step 2)
Extraction operation is dynamic gradient counter-current extraction in the step 2), and dimethyl ether fluid continues through one group of extraction successively
Tank, dimethyl ether con steps up in single tank, and the front end list tank for completing extraction operation is detached from extracting system, new to load broken wall algal gel
Single tank be connected into the end of extracting system, dimethyl ether fluid and broken wall algal gel reverse flow.
The extractor group is made of 5 one-pot tandems.
The pressure of extract liquor precipitation is 0.08MPa in the step 3), and temperature is 60 DEG C.
Output fucoxanthin after thick fucoxanthin grease centrifuges after the precipitation, centrifuge speed are 8000 revs/min
Clock.
Embodiment three：
A kind of method of dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin, includes step：
1) the fresh algae freeze thawing broken wall of Phaeodactylum tricornutum；
2) dimethyl ether fluid dynamic gradient counter-current extraction broken wall algal gel；
3) extract liquor precipitation isolates fucoxanthin.
The fresh algae of step 1) intermediate cam brown algae is the fresh algae that Phaeodactylum tricornutum is dried through supercentrifuge, containing water quality
Percentage is 5.0%.
The fresh algae of Phaeodactylum tricornutum is first quick-frozen, then refrigerates, and finally thaws and realizes freeze thawing broken wall, and quick freezing temperature is -40
DEG C, the quick-frozen time is 12.0h, and -10 DEG C of refrigerated storage temperature, thaw point is 30 DEG C, thawing time 1.5h, number of freezing and thawing 3
It is secondary.
Dimethyl ether fluid is subcritical fluids in the step 2), and pressure 1.0MPa, temperature is 60 DEG C.
The residence time of dimethyl ether fluid is 2.0 hours in the step 2)
Extraction operation is dynamic gradient counter-current extraction in the step 2), and dimethyl ether fluid continues through one group of extraction successively
Tank, dimethyl ether con steps up in single tank, and the front end list tank for completing extraction operation is detached from extracting system, new to load broken wall algal gel
Single tank be connected into the end of extracting system, dimethyl ether fluid and broken wall algal gel reverse flow.
The extractor group is made of 6 one-pot tandems.
The pressure 0.10MPa of extract liquor precipitation in the step 3), temperature are 70 DEG C.
Output fucoxanthin after thick fucoxanthin grease centrifuges after the precipitation, centrifuge speed are 10000 revs/min
Clock.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (9)
- A kind of 1. method of dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin, which is characterized in that including step：1) the fresh algae freeze thawing broken wall of Phaeodactylum tricornutum；2) dimethyl ether fluid dynamic gradient counter-current extraction broken wall algal gel；3) extract liquor precipitation isolates fucoxanthin.
- 2. the method for dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin as described in claim 1, it is characterised in that：It is described The fresh algae of step 1) intermediate cam brown algae is the fresh algae that Phaeodactylum tricornutum is dried through supercentrifuge, and aqueous mass percent is 65.0%~75.0%.
- 3. the method for dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin as claimed in claim 2, it is characterised in that：It is described The fresh algae of Phaeodactylum tricornutum is first quick-frozen, then refrigerates, and finally thaws and realizes freeze thawing broken wall, and quick freezing temperature is -70 DEG C~-40 DEG C, The quick-frozen time is 8.0h~12.0h, and -20 DEG C~-10 DEG C of refrigerated storage temperature, thaw point is 20 DEG C~30 DEG C, and thawing time is 0.5~1.5h, number of freezing and thawing are 1~3 time.
- 4. the method for dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin as described in claim 1, it is characterised in that：It is described Dimethyl ether fluid is subcritical fluids in step 2), and pressure is 0.5MPa~1.0MPa, and temperature is 40 DEG C~60 DEG C.
- 5. the method for dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin as claimed in claim 4, it is characterised in that：It is described The residence time of dimethyl ether fluid is 1.0~2.0 hours in step 2).
- 6. the method for dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin as described in claim 1, it is characterised in that：It is described Extraction operation is dynamic gradient counter-current extraction in step 2), and dimethyl ether fluid continues through one group of extractor successively, two in single tank Methyl ether concentration steps up, and the front end list tank for completing extraction operation is detached from extracting system, and the new single tank for loading broken wall algal gel is connected into The end of extracting system, dimethyl ether fluid and broken wall algal gel reverse flow.
- 7. the method for dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin as claimed in claim 6, it is characterised in that：It is described Extractor group is made of 4~6 one-pot tandems.
- 8. the method for dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin as described in claim 1, it is characterised in that：It is described The pressure of extract liquor precipitation is 0.05MPa~0.10MPa in step 3), and temperature is 50 DEG C~70 DEG C.
- 9. the method for dimethyl ether fluid extraction Phaeodactylum tricornutum fucoxanthin as claimed in claim 8, it is characterised in that：It is described Output fucoxanthin after thick fucoxanthin grease centrifuges after precipitation, centrifuge speed for 4500 revs/min~10000 turns/ Minute.
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| CN111560035A (en) * | 2020-05-15 | 2020-08-21 | 河海大学 | Method for extracting phosphate from algae mud by using dimethyl ether |
| CN112034063A (en) * | 2020-09-02 | 2020-12-04 | 苏州华测生物技术有限公司 | Method for measuring concentration of zanthoxylum schinifolium alkali in biological matrix |
| CN113769437A (en) * | 2021-10-22 | 2021-12-10 | 武汉轻工大学 | Method for rapidly extracting high-quality bioactive components from microalgae cells and application thereof |
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| CN103965143A (en) * | 2013-01-30 | 2014-08-06 | 中国科学院海洋研究所 | Method for extracting and separating fucoxanthin from marine unicellular diatom |
| CN105001184A (en) * | 2015-06-17 | 2015-10-28 | 宁波大学 | Pretreatment method of brown alga samples for fucoxanthin extraction |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111560035A (en) * | 2020-05-15 | 2020-08-21 | 河海大学 | Method for extracting phosphate from algae mud by using dimethyl ether |
| CN112034063A (en) * | 2020-09-02 | 2020-12-04 | 苏州华测生物技术有限公司 | Method for measuring concentration of zanthoxylum schinifolium alkali in biological matrix |
| CN113769437A (en) * | 2021-10-22 | 2021-12-10 | 武汉轻工大学 | Method for rapidly extracting high-quality bioactive components from microalgae cells and application thereof |
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| CN108129420B (en) | 2021-07-30 |
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