CN108125986A - The extracting method of effective ingredients from ants - Google Patents
The extracting method of effective ingredients from ants Download PDFInfo
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- CN108125986A CN108125986A CN201711412850.2A CN201711412850A CN108125986A CN 108125986 A CN108125986 A CN 108125986A CN 201711412850 A CN201711412850 A CN 201711412850A CN 108125986 A CN108125986 A CN 108125986A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D11/02—Solvent extraction of solids
- B01D11/028—Flow sheets
- B01D11/0284—Multistage extraction
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D11/00—Solvent extraction
- B01D2011/005—Co-current extraction
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Abstract
The invention discloses a kind of extracting method of effective ingredients from ants, including：Step 1) chooses artificial breeding or wild complete ant polypide；Ant polypide is placed in drying equipment by step 2)；Ant polypide is put into carbon dioxide abstraction device by step 3), successively carries out two secondary cell wall broken wall treatments；Ant polypide is continued to be placed in carbon dioxide abstraction device by step 4), is extracted, and entire extraction process is divided into 5 stages, and from first stage to a last stage, the flow of carbon dioxide successively increases；After step 5) extracts, extraction fluid is sent into separator and is detached, wherein, the level-one separating pressure of separator is 20 25MPa, separation temperature is 50 55 DEG C, and the second-order separation pressure is 10 15MPa, and separation temperature is 50 55 DEG C, three-level separating pressure is 5 8MPa, and separation temperature is 50 55 DEG C.The present invention improves the yield of active ingredient.
Description
Technical field
The present invention relates to agriculture field more particularly to a kind of extracting methods of effective ingredients from ants.
Background technology
Ant contains abundant business substance, has good medical value.Research shows that ant powder to it is anti-inflammatory, analgesia,
Sterilization, cancer-resisting improve immunity etc. and are respectively provided with pharmacological action.But traditional ant processing method is also mainly with powder
It is broken, mill with organic solvent extraction based on.Crush silt, leaf or the grass that cannot be removed effectively with method for grinding with ant
The impurity such as bits, and there are the problems such as heavy metals exceeding standard, some bioactive substances also easily lose in process.It is and organic
Although solvent extraction method can extract the active ingredient in ant, there are problems that dissolvent residual, influence product quality, it is dirty
Environment is contaminated, and the production cycle is long, high energy consumption.
Invention content
It is an object of the invention to solve at least the above, and provide the advantages of at least will be described later.
It is a still further object of the present invention to provide a kind of extracting method of effective ingredients from ants, including：
Step 1) chooses artificial breeding or wild complete ant polypide, and ant polypide is cleaned up with clear water；
Treated that ant polypide is placed in drying equipment by the step 1) for step 2), is passed through 90-100 DEG C of hot wind,
3-4h is continuously fed to, is re-fed into the wind of room temperature, so as to which the ant polypide for making drying restores to room temperature；
The ant polypide that the step 2) is prepared is put into carbon dioxide abstraction device by step 3), successively carries out twice
Cell wall broken wall treatment, wherein, during the first secondary cell wall break process, cell wall broken wall pressure be 10-12MPa, broken wall
Temperature be 25-30 DEG C, time 5-10min, then by pressure reducer by the pressure reduction in the carbon dioxide abstraction device extremely
5MPa stands 20-30min, then carries out the second secondary cell wall broken wall treatment, during the second secondary cell wall break process,
Cell wall broken wall pressure is 35-40MPa, and broken wall temperature is 45-55 DEG C, time 60-80min；
Ant polypide Jing Guo two secondary cell wall broken wall treatments in the step 3) is continued to be placed on titanium dioxide by step 4)
In carbon extractor, extracted, wherein, extracting pressure 38-40MPa, extraction temperature is 50-55 DEG C, by entire extraction process
4 stages are divided into, from first stage to a last stage, the flow of carbon dioxide successively increases, detailed process packet
It includes：
In first stage, the flow of carbon dioxide is 5-7m3/ h, duration 30-40min, in second stage,
The flow of carbon dioxide is 10-13m3/ h, duration 1-2h, in three phases, the flow of carbon dioxide is 18-20m3/
H, duration 1-2h, in four-stage, the flow of carbon dioxide is 40-50m3/ h, duration 3-4h；
After step 5) extracts, the extraction fluid that the step 4) is prepared is sent into separator and is detached,
Wherein, the level-one separating pressure of separator is 20-25MPa, and separation temperature is 50-55 DEG C, disengaging time 5-10min, separation
The second-order separation pressure of device is 10-15MPa, and separation temperature is 50-55 DEG C, disengaging time 5-10min, three fractions of separator
Tripping power is 5-8MPa, and separation temperature is 50-55 DEG C, disengaging time 5-10min；
After step 6) detaches, the temperature in the separator is controlled to drop to room temperature with the speed of 0.5-1 DEG C/min,
Extraction product is taken out out of described separator；
The extraction product that the step 6) is prepared is filled into container by step 7), seals the container protect later
It deposits.
Preferably, in the extracting method of the effective ingredients from ants, the type of the ant polypide is red woods ant, red
Ant polypide or Emery formicusina.
Preferably, in the extracting method of the effective ingredients from ants, in the step 2), the first secondary cell wall crushes
In processing procedure, cell wall broken wall pressure is 10MPa, and broken wall temperature is 25 DEG C, time 5min, then by pressure reducer by institute
The pressure reduction in carbon dioxide abstraction device is stated to 5MPa, stands 20min.
Preferably, in the extracting method of the effective ingredients from ants, in the step 2), then second of cell is carried out
Wall broken wall treatment, during the second secondary cell wall break process, cell wall broken wall pressure is 35MPa, and broken wall temperature is 45
DEG C, time 60min.
Preferably, in the extracting method of the effective ingredients from ants, in the step 3), the level-one separation of separator
Pressure is 20MPa, and separation temperature is 50 DEG C, disengaging time 5min, and the second-order separation pressure of separator is 10MPa, and separation is warm
It is 50 DEG C, disengaging time 5min to spend, and the three-level separating pressure of separator is 5MPa, and separation temperature is 50 DEG C, and disengaging time is
5min。
Preferably, in the extracting method of the effective ingredients from ants, in the step 3), after separation, control
Temperature in separator drops to room temperature with the speed of 0.5 DEG C/min.
The present invention includes at least following advantageous effect：
A kind of extracting method of effective ingredients from ants provided by the invention, including：Step 1) chooses artificial breeding or wild
Raw complete ant polypide cleans up ant polypide with clear water；Step 2) is by the step 1) treated ant polypide
It is placed in drying equipment, is passed through 90-100 DEG C of hot wind, is continuously fed to 3-4h, be re-fed into the wind of room temperature, so as to make drying
Ant polypide restores to room temperature；The ant polypide that the step 2) is prepared is put into carbon dioxide abstraction device by step 3),
Two secondary cell wall broken wall treatments are successively carried out, wherein, during the first secondary cell wall break process, cell wall broken wall pressure is
10-12MPa, broken wall temperature are 25-30 DEG C, time 5-10min, then will be in the carbon dioxide abstraction device by pressure reducer
Pressure reduction to 5MPa, stand 20-30min, then carry out the second secondary cell wall broken wall treatment, second secondary cell wall crushes
In processing procedure, cell wall broken wall pressure is 35-40MPa, and broken wall temperature is 45-55 DEG C, time 60-80min；Step 4) will
Ant polypide in the step 3) Jing Guo two secondary cell wall broken wall treatments continues to be placed in carbon dioxide abstraction device, is extracted
It takes, wherein, extracting pressure 38-40MPa, extraction temperature is 50-55 DEG C, and entire extraction process is divided into 4 stages, from the
One stage to a last stage, the flow of carbon dioxide successively increase, and detailed process includes：In first stage, two
The flow of carbonoxide is 5-7m3/ h, duration 30-40min, in second stage, the flow of carbon dioxide is 10-13m3/
H, duration 1-2h, in three phases, the flow of carbon dioxide is 18-20m3/ h, duration 1-2h, the 4th
In a stage, the flow of carbon dioxide is 40-50m3/ h, duration 3-4h；After step 5) extracts, by the step 4)
The extraction fluid being prepared is sent into separator and is detached, wherein, the level-one separating pressure of separator is 20-25MPa, point
It it is 50-55 DEG C, disengaging time 5-10min from temperature, the second-order separation pressure of separator is 10-15MPa, and separation temperature is
50-55 DEG C, disengaging time 5-10min, the three-level separating pressure of separator is 5-8MPa, and separation temperature is 50-55 DEG C, separation
Time is 5-10min；After step 6) detaches, the temperature in the separator is controlled to decline with the speed of 0.5-1 DEG C/min
To room temperature, extraction product is taken out out of described separator；The extraction product that the step 6) is prepared step 7) is filling
Enter in container, be later sealed the container.The present invention using supercritical carbon dioxide method for extracting to ant polypide into
Row breaking-wall cell, allows active ingredient fully to release, and is further extracted using supercritical carbon dioxide method for extracting
Separating treatment is taken, destruction of the mechanical crushing to active ingredient is avoided, utmostly remains active ingredient, improve active ingredient
Yield.
Part is illustrated to embody by further advantage, target and the feature of the present invention by following, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Specific embodiment
The present invention is described in further detail below, to enable those skilled in the art being capable of evidence with reference to specification word
To implement.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein do not allot one or more
The presence or addition of a other element or combinations.
Embodiment 1
Step 1) chooses artificial breeding or wild complete ant polypide, and ant polypide is cleaned up with clear water；Institute
The type for stating ant polypide is red woods ant；
Treated that ant polypide is placed in drying equipment by the step 1) for step 2), is passed through 90 DEG C of hot wind, continues
3h is sent into, the wind of room temperature is re-fed into, so as to which the ant polypide for making drying restores to room temperature；
The ant polypide that the step 2) is prepared is put into carbon dioxide abstraction device by step 3), successively carries out twice
Cell wall broken wall treatment, wherein, during the first secondary cell wall break process, cell wall broken wall pressure be 10MPa, broken wall temperature
It is 25 DEG C, time 5min, then by pressure reducer by the pressure reduction in the carbon dioxide abstraction device to 5MPa, stands
20min, then the second secondary cell wall broken wall treatment is carried out, during the second secondary cell wall break process, cell wall broken wall pressure
Power is 35MPa, and broken wall temperature is 45 DEG C, time 60min；
Ant polypide Jing Guo two secondary cell wall broken wall treatments in the step 3) is continued to be placed on titanium dioxide by step 4)
In carbon extractor, extracted, wherein, extracting pressure 38MPa, extraction temperature is 50 DEG C, and entire extraction process is divided into 4
In a stage, from first stage to a last stage, the flow of carbon dioxide successively increases, and detailed process includes：
In first stage, the flow of carbon dioxide is 5m3/ h, duration 30min, in second stage, dioxy
The flow for changing carbon is 10m3/ h, duration 1h, in three phases, the flow of carbon dioxide is 18m3/ h, duration be
1h, in four-stage, the flow of carbon dioxide is 40m3/ h, duration 3h；
After step 5) extracts, the extraction fluid that the step 4) is prepared is sent into separator and is detached,
Wherein, the level-one separating pressure of separator is 20MPa, and separation temperature is 50 DEG C, disengaging time 5min, two fractions of separator
Tripping power is 10MPa, and separation temperature is 50 DEG C, disengaging time 5min, and the three-level separating pressure of separator is 5MPa, and separation is warm
It is 50 DEG C to spend, disengaging time 5min；
After step 6) detaches, the temperature in the separator is controlled to drop to room temperature with the speed of 0.5 DEG C/min, it will
Extraction product takes out out of described separator；
The extraction product that the step 6) is prepared is filled into container by step 7), seals the container protect later
It deposits.
Embodiment 2
Step 1) chooses artificial breeding or wild complete ant polypide, and ant polypide is cleaned up with clear water；Institute
The type for stating ant polypide is red ant；
Treated that ant polypide is placed in drying equipment by the step 1) for step 2), is passed through 100 DEG C of hot wind, continues
4h is sent into, the wind of room temperature is re-fed into, so as to which the ant polypide for making drying restores to room temperature；
The ant polypide that the step 2) is prepared is put into carbon dioxide abstraction device by step 3), successively carries out twice
Cell wall broken wall treatment, wherein, during the first secondary cell wall break process, cell wall broken wall pressure be 12MPa, broken wall temperature
It is 30 DEG C, time 10min, then by pressure reducer by the pressure reduction in the carbon dioxide abstraction device to 5MPa, stands
30min, then the second secondary cell wall broken wall treatment is carried out, during the second secondary cell wall break process, cell wall broken wall pressure
Power is 40MPa, and broken wall temperature is 55 DEG C, time 80min；
Ant polypide Jing Guo two secondary cell wall broken wall treatments in the step 3) is continued to be placed on titanium dioxide by step 4)
In carbon extractor, extracted, wherein, extracting pressure 40MPa, extraction temperature is 55 DEG C, and entire extraction process is divided into 4
In a stage, from first stage to a last stage, the flow of carbon dioxide successively increases, and detailed process includes：
In first stage, the flow of carbon dioxide is 7m3/ h, duration 40min, in second stage, dioxy
The flow for changing carbon is 13m3/ h, duration 2h, in three phases, the flow of carbon dioxide is 20m3/ h, duration be
2h, in four-stage, the flow of carbon dioxide is 50m3/ h, duration 4h；
After step 5) extracts, the extraction fluid that the step 4) is prepared is sent into separator and is detached,
Wherein, the level-one separating pressure of separator is 25MPa, and separation temperature is 55 DEG C, disengaging time 10min, the two level of separator
Separating pressure is 15MPa, and separation temperature is 55 DEG C, disengaging time 10min, and the three-level separating pressure of separator is 8MPa, point
It is 55 DEG C from temperature, disengaging time 10min；
After step 6) detaches, the temperature in the separator is controlled to drop to room temperature with the speed of 1 DEG C/min, will be extracted
Product is taken to be taken out out of described separator；
The extraction product that the step 6) is prepared is filled into container by step 7), seals the container protect later
It deposits.
Embodiment 3
Step 1) chooses artificial breeding or wild complete ant polypide, and ant polypide is cleaned up with clear water；Institute
The type for stating ant polypide is Emery formicusina；
Treated that ant polypide is placed in drying equipment by the step 1) for step 2), is passed through 100 DEG C of hot wind, continues
4h is sent into, the wind of room temperature is re-fed into, so as to which the ant polypide for making drying restores to room temperature；
The ant polypide that the step 2) is prepared is put into carbon dioxide abstraction device by step 3), successively carries out twice
Cell wall broken wall treatment, wherein, during the first secondary cell wall break process, cell wall broken wall pressure be 10MPa, broken wall temperature
It is 25 DEG C, time 5min, then by pressure reducer by the pressure reduction in the carbon dioxide abstraction device to 5MPa, stands
30min, then the second secondary cell wall broken wall treatment is carried out, during the second secondary cell wall break process, cell wall broken wall pressure
Power is 40MPa, and broken wall temperature is 55 DEG C, time 80min；
Ant polypide Jing Guo two secondary cell wall broken wall treatments in the step 3) is continued to be placed on titanium dioxide by step 4)
In carbon extractor, extracted, wherein, extracting pressure 40MPa, extraction temperature is 55 DEG C, and entire extraction process is divided into 4
In a stage, from first stage to a last stage, the flow of carbon dioxide successively increases, and detailed process includes：
In first stage, the flow of carbon dioxide is 5m3/ h, duration 30min, in second stage, dioxy
The flow for changing carbon is 113m3/ h, duration 2h, in three phases, the flow of carbon dioxide is 20m3/ h, duration
For 2h, in four-stage, the flow of carbon dioxide is 50m3/ h, duration 4h；
After step 5) extracts, the extraction fluid that the step 4) is prepared is sent into separator and is detached,
Wherein, the level-one separating pressure of separator be 25MPa, separation temperature be 55 DEG C, disengaging time 5-10min, the two of separator
Grade separating pressure is 15MPa, and separation temperature is 55 DEG C, disengaging time 10min, and the three-level separating pressure of separator is 8MPa,
Separation temperature is 55 DEG C, disengaging time 10min；
After step 6) detaches, the temperature in the separator is controlled to drop to room temperature with the speed of 1 DEG C/min, will be extracted
Product is taken to be taken out out of described separator；
The extraction product that the step 6) is prepared is filled into container by step 7), seals the container protect later
It deposits.
Embodiment 4
Step 1) chooses artificial breeding or wild complete ant polypide, and ant polypide is cleaned up with clear water；Institute
The type for stating ant polypide is Emery formicusina；
Treated that ant polypide is placed in drying equipment by the step 1) for step 2), is passed through 95 DEG C of hot wind, continues
3h is sent into, the wind of room temperature is re-fed into, so as to which the ant polypide for making drying restores to room temperature；
The ant polypide that the step 2) is prepared is put into carbon dioxide abstraction device by step 3), successively carries out twice
Cell wall broken wall treatment, wherein, during the first secondary cell wall break process, cell wall broken wall pressure be 12MPa, broken wall temperature
It is 26 DEG C, time 7min, then by pressure reducer by the pressure reduction in the carbon dioxide abstraction device to 5MPa, stands
20-30min, then the second secondary cell wall broken wall treatment is carried out, during the second secondary cell wall break process, cell wall broken wall
Pressure is 37MPa, and broken wall temperature is 45 DEG C, time 65min；
Ant polypide Jing Guo two secondary cell wall broken wall treatments in the step 3) is continued to be placed on titanium dioxide by step 4)
In carbon extractor, extracted, wherein, extracting pressure 40MPa, extraction temperature is 55 DEG C, and entire extraction process is divided into 4
In a stage, from first stage to a last stage, the flow of carbon dioxide successively increases, and detailed process includes：
In first stage, the flow of carbon dioxide is 7m3/ h, duration 40min, in second stage, dioxy
The flow for changing carbon is 13m3/ h, duration 2h, in three phases, the flow of carbon dioxide is 18m3/ h, duration be
1h, in four-stage, the flow of carbon dioxide is 45m3/ h, duration 3h；
After step 5) extracts, the extraction fluid that the step 4) is prepared is sent into separator and is detached,
Wherein, the level-one separating pressure of separator is 20MPa, and separation temperature is 50 DEG C, disengaging time 5min, two fractions of separator
Tripping power is 10MPa, and separation temperature is 50 DEG C, disengaging time 10min, and the three-level separating pressure of separator is 5MPa, is detached
Temperature is 50 DEG C, disengaging time 5min；
After step 6) detaches, the temperature in the separator is controlled to drop to room temperature with the speed of 1 DEG C/min, will be extracted
Product is taken to be taken out out of described separator；
The extraction product that the step 6) is prepared is filled into container by step 7), seals the container protect later
It deposits.
Embodiment 5
Step 1) chooses artificial breeding or wild complete ant polypide, and ant polypide is cleaned up with clear water；Institute
The type for stating ant polypide is red woods ant；
Treated that ant polypide is placed in drying equipment by the step 1) for step 2), is passed through 100 DEG C of hot wind, continues
3h is sent into, the wind of room temperature is re-fed into, so as to which the ant polypide for making drying restores to room temperature；
The ant polypide that the step 2) is prepared is put into carbon dioxide abstraction device by step 3), successively carries out twice
Cell wall broken wall treatment, wherein, during the first secondary cell wall break process, cell wall broken wall pressure be 10MPa, broken wall temperature
It is 25 DEG C, time 5min, then by pressure reducer by the pressure reduction in the carbon dioxide abstraction device to 5MPa, stands
30min, then the second secondary cell wall broken wall treatment is carried out, during the second secondary cell wall break process, cell wall broken wall pressure
Power is 40MPa, and broken wall temperature is 45 DEG C, time 65min；
Ant polypide Jing Guo two secondary cell wall broken wall treatments in the step 3) is continued to be placed on titanium dioxide by step 4)
In carbon extractor, extracted, wherein, extracting pressure 40MPa, extraction temperature is 55 DEG C, and entire extraction process is divided into 4
In a stage, from first stage to a last stage, the flow of carbon dioxide successively increases, and detailed process includes：
In first stage, the flow of carbon dioxide is 5m3/ h, duration 35min, in second stage, dioxy
The flow for changing carbon is 10m3/ h, duration 1h, in three phases, the flow of carbon dioxide is 20m3/ h, duration be
2h, in four-stage, the flow of carbon dioxide is 50m3/ h, duration 4h；
After step 5) extracts, the extraction fluid that the step 4) is prepared is sent into separator and is detached,
Wherein, the level-one separating pressure of separator is 20MPa, and separation temperature is 52 DEG C, disengaging time 5min, two fractions of separator
Tripping power is 15MPa, and separation temperature is 52 DEG C, disengaging time 5min, and the three-level separating pressure of separator is 8MPa, and separation is warm
It is 52 DEG C to spend, disengaging time 5min；
After step 6) detaches, the temperature in the separator is controlled to drop to room temperature with the speed of 1 DEG C/min, will be extracted
Product is taken to be taken out out of described separator；
The extraction product that the step 6) is prepared is filled into container by step 7), seals the container protect later
It deposits.
Comparative example：
Active ingredient in ant is extracted using existing organic solvent extraction, handles the ant of 1000g, extraction
The index of active ingredient is shown in Table 1.
Compliance test result：
The extraction of active ingredient is carried out to 5000g ants using the method for embodiment 1 to embodiment 5.Wherein, it is each to implement
Example processing 1000g.The index of the active ingredient for the ant that embodiment 1 is extracted to embodiment 5 is shown in Table 1.The present invention improves pair
The yield of active ingredient in ant.
Table 1
| Embodiment | Active ingredient g) | Yield %) |
| Comparative example | 100.2 | 10.2 |
| Embodiment 1 | 204.5 | 20.45 |
| Embodiment 2 | 209.1 | 20.91 |
| Embodiment 3 | 208.2 | 20.82 |
| Embodiment 4 | 207.7 | 20.77 |
| Embodiment 5 | 207.9 | 20.79 |
Although the embodiments of the present invention have been disclosed as above, but its be not restricted in specification and embodiment it is listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, it is of the invention and unlimited
In specific details.
Claims (6)
1. a kind of extracting method of effective ingredients from ants, which is characterized in that including：
Step 1) chooses artificial breeding or wild complete ant polypide, and ant polypide is cleaned up with clear water；
Treated that ant polypide is placed in drying equipment by the step 1) for step 2), is passed through 90-100 DEG C of hot wind, continues
3-4h is sent into, the wind of room temperature is re-fed into, so as to which the ant polypide for making drying restores to room temperature；
The ant polypide that the step 2) is prepared is put into carbon dioxide abstraction device by step 3), successively carries out cell twice
Wall broken wall treatment, wherein, during the first secondary cell wall break process, cell wall broken wall pressure be 10-12MPa, broken wall temperature
It is 25-30 DEG C, time 5-10min, then by pressure reducer by the pressure reduction in the carbon dioxide abstraction device to 5MPa,
20-30min is stood, then carries out the second secondary cell wall broken wall treatment, during the second secondary cell wall break process, cell wall
Broken wall pressure is 35-40MPa, and broken wall temperature is 45-55 DEG C, time 60-80min；
Step 4) continues the ant polypide Jing Guo two secondary cell wall broken wall treatments in the step 3) to be placed on carbon dioxide extraction
It takes in device, is extracted, wherein, extracting pressure 38-40MPa, extraction temperature is 50-55 DEG C, and entire extraction process is divided
For 4 stages, from first stage to a last stage, the flow of carbon dioxide successively increases, and detailed process includes：
In first stage, the flow of carbon dioxide is 5-7m3/ h, duration 30-40min, in second stage, dioxy
The flow for changing carbon is 10-13m3/ h, duration 1-2h, in three phases, the flow of carbon dioxide is 18-20m3/ h, holds
The continuous time is 1-2h, and in four-stage, the flow of carbon dioxide is 40-50m3/ h, duration 3-4h；
After step 5) extracts, the extraction fluid that the step 4) is prepared is sent into separator and is detached, wherein,
The level-one separating pressure of separator be 20-25MPa, separation temperature be 50-55 DEG C, disengaging time 5-10min, the two of separator
Grade separating pressure is 10-15MPa, and separation temperature is 50-55 DEG C, disengaging time 5-10min, the three-level separating pressure of separator
For 5-8MPa, separation temperature is 50-55 DEG C, disengaging time 5-10min；
After step 6) detaches, the temperature in the separator is controlled to drop to room temperature with the speed of 0.5-1 DEG C/min, will be extracted
Product is taken to be taken out out of described separator；
The extraction product that the step 6) is prepared is filled into container by step 7), is later sealed the container.
2. the extracting method of effective ingredients from ants as described in claim 1, which is characterized in that the type of the ant polypide is
Red woods ant, red ant polypide or Emery formicusina.
3. the extracting method of effective ingredients from ants as described in claim 1, which is characterized in that in the step 2), for the first time
During cell wall break process, cell wall broken wall pressure is 10MPa, and broken wall temperature is 25 DEG C, then time 5min passes through
Pressure reduction in the carbon dioxide abstraction device to 5MPa is stood 20min by pressure reducer.
4. the extracting method of effective ingredients from ants as described in claim 1, which is characterized in that in the step 2), then carry out
Second secondary cell wall broken wall treatment, during the second secondary cell wall break process, cell wall broken wall pressure is 35MPa, is broken
Wall temperature is 45 DEG C, time 60min.
5. the extracting method of effective ingredients from ants as described in claim 1, which is characterized in that in the step 3), separator
Level-one separating pressure for 20MPa, separation temperature is 50 DEG C, disengaging time 5min, and the second-order separation pressure of separator is
10MPa, separation temperature are 50 DEG C, disengaging time 5min, and the three-level separating pressure of separator is 5MPa, separation temperature 50
DEG C, disengaging time 5min.
6. the extracting method of effective ingredients from ants as described in claim 1, which is characterized in that in the step 3), separation knot
Shu Hou controls the temperature in separator to drop to room temperature with the speed of 0.5 DEG C/min.
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| CN201711412850.2A CN108125986A (en) | 2017-12-24 | 2017-12-24 | The extracting method of effective ingredients from ants |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1557924A (en) * | 2004-01-12 | 2004-12-29 | 浙江大学 | Supercritical CO2 extraction process for preparing ant oil |
| CN1883511A (en) * | 2006-05-29 | 2006-12-27 | 南京中山制药有限公司 | Inclusion compound of ants supercritical extracts and preparation process thereof |
| CN101007023A (en) * | 2006-01-26 | 2007-08-01 | 山西亚宝药业集团股份有限公司 | Extraction method of effective ingredients from ants |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1557924A (en) * | 2004-01-12 | 2004-12-29 | 浙江大学 | Supercritical CO2 extraction process for preparing ant oil |
| CN101007023A (en) * | 2006-01-26 | 2007-08-01 | 山西亚宝药业集团股份有限公司 | Extraction method of effective ingredients from ants |
| CN1883511A (en) * | 2006-05-29 | 2006-12-27 | 南京中山制药有限公司 | Inclusion compound of ants supercritical extracts and preparation process thereof |
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