CN108117979A - A kind of kit based on the detection of EFIRM technologies HPV viruse - Google Patents
A kind of kit based on the detection of EFIRM technologies HPV viruse Download PDFInfo
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- CN108117979A CN108117979A CN201810002945.5A CN201810002945A CN108117979A CN 108117979 A CN108117979 A CN 108117979A CN 201810002945 A CN201810002945 A CN 201810002945A CN 108117979 A CN108117979 A CN 108117979A
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- fixedly connected
- box body
- reagent box
- detection
- efirm
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
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- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Virology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a kind of kits based on the detection of EFIRM technologies HPV viruse, driving shaft lower part rolls connection transmission belt right end, transmission belt left end rolls connection wire rod lower part, screw top is threadedly coupled Step Shaft, Step Shaft is socketed sleeve, and Step Shaft upper end is fixedly connected with transverse slat left end, and transverse slat right part bearing is rotatablely connected vertical strut, vertical strut socket reagent box body, and transverse slat right end is fixedly connected with visor cylinder.The kit rotates driving bottom plate by driving shaft and parcel shelf synchronous rotation shakes up the invisible spectro blood to be detected of storage, sample is sealed in storage room by cover board, prevent sample for a long time exposed to outer infection, peephole is always in reagent box body, it avoids being infected with germs, and rotate the amplification that adjusting knob drives the peephole of different sighting distances and thickness alignment glass slide and colorimetric card can realize different multiples so that detection is more accurate.Reagent cartridge configuration design is rationally applicable in, and substantially increases the accuracy of detection.
Description
Technical field
The present invention relates to a kind of medical auxiliary tools, are specifically a kind of reagent based on the detection of EFIRM technologies HPV viruse
Box.
Background technology
HPV viruse is human papilloma virus, is that a kind of papilloma vacuolating virus A for belonging to papovaviridae belongs to, is
Spherical DNA virus can cause the scaly epithelium of human skin mucous membrane to be proliferated.Show as verruca vulgaris, genital wart i.e. condyloma acuminatum
Etc. symptoms.Increase as the incidence of condyloma acuminatum in venereal disease rapidly rises with cervical carcinoma, cancer of anus etc., human papilloma virus
Infection increasingly causes the concern of people.
EFIRM technologies are precisely detected using the noninvasive gene of liquid biopsy, directly detect the gene mutation information in blood, whole
A process is quick, convenient, and perfect adaptation detecting instrument realizes quick detection process, without tumor tissues sample.
Current existing EFIRM technologies use cost is higher, is usually required before using EFIRM technologies precisely detection preliminary
Detection, typically using gene detecting kit, the gene corresponding detected sample being put into gene detecting kit
It is detected after chip dyeing, being needed when being then compared, and comparing with special colorimetric card could be clear after being amplified
Ground observation compares, and sample is caused testing result precision not by existing kit in detection process exposed to outer easily infection
Height, and blood plasma, red blood cell, leucocyte, blood platelet in multiple blood sample standing time longer blood to be checked etc. can separate
Precipitation, is unfavorable for detecting, and for convex lens exposed to can also infect blood outside, detection result is bad.
The content of the invention
It is an object of the invention to provide a kind of kit based on the detection of EFIRM technologies HPV viruse, to solve the above-mentioned back of the body
The problem of being proposed in scape technology.
To achieve the above object, the present invention provides following technical solution:
A kind of kit based on the detection of EFIRM technologies HPV viruse, including reagent box body;The reagent box body is mainly
It is made of storage room, driving shaft, screw, monitor station, peephole and visor cylinder, storage room is set on the right side of the reagent box body,
Hinged closures on the right side of storage room upper end, cover board left end set bolt, abut spring on the right side of bolt, spring is fixedly connected with cover board;Institute
It states cover board lower part and parcel shelf, parcel shelf both sides rotation connection idler wheel is set, idler wheel rolls connection storage chamber interior walls, parcel shelf clamping
Storage room;Driving shaft upper end is fixedly connected in the middle part of the parcel shelf lower end, parcel shelf lower part sets bottom plate, and bottom plate is fixedly connected with master
Moving axis, driving shaft lower end rotation connection driving motor, driving motor are fixedly connected with kit body bottom portion;The driving shaft lower part
Connection transmission belt right end is rolled, transmission belt left end rolls connection wire rod lower part, screw bottom bearing rotation connection reagent box body
Bottom, screw top is threadedly coupled Step Shaft, Step Shaft socket sleeve, and sleeve is fixedly connected with kit body upper part;It is described
Step Shaft upper end is fixedly connected with transverse slat left end, and transverse slat right part bearing is rotatablely connected vertical strut, and vertical strut upper end is fixedly connected
Adjusting knob, vertical strut are socketed reagent box body, and vertical strut lower circumference is layered the peephole that is interspersed, and peephole is convex
Lens, and transverse slat right end is fixedly connected with visor cylinder;The screw lower part is fixedly connected with gear, gear pinion rack, and rack is fixed
Connecting detection platform, monitor station right end are slidably connected reagent box body side wall, and monitor station left upper part is fixedly connected with the lower end of the slider, sliding
Bar upper end is slidably connected reagent box body, and monitor station upper surface sets glass slide, and colorimetric card is set on the right side of glass slide.
As further embodiment of the present invention:The visor cylinder lower end sets glass plate, and glass plate is fixedly connected with kit
Body.
As further scheme of the invention:The parcel shelf is circumferentially uniformly arranged multiple jacks, and parcel shelf is soft
Rubber material is made.
As further scheme of the invention:The reagent box body both sides lower end is fixedly connected with supporting leg.
As further scheme of the invention:Two lantern rings of grafting, left end lantern ring fix company respectively on the left of the bolt
Reagent box body is connect, right end lantern ring is fixedly connected with cover board.
As further scheme of the invention:The driving motor is electrically connected power supply and switch.
As further scheme of the invention:The Step Shaft outer surface sets raised line, and sleeve interior surface sets recessed bar.
Compared with prior art, the beneficial effects of the invention are as follows:The kit rotates driving bottom plate by driving shaft and puts
Object plate synchronous rotation shakes up the invisible spectro blood to be detected of storage, while gear drives monitor station scaling convenient for addition blood
Sample is sealed in storage room by liquid, cover board, prevents sample for a long time exposed to outer infection, peephole is in kit sheet always
In vivo, avoid being infected with germs, and rotate adjusting knob and drive the peephole of different sighting distances and thickness alignment glass slide and colorimetric card
The amplification of different multiples can be achieved so that detection is more accurate.Reagent cartridge configuration design is rationally applicable in, and substantially increases detection
Accuracy.
Description of the drawings
Fig. 1 is the structure diagram of the kit based on the detection of EFIRM technologies HPV viruse.
Fig. 2 is the top view of vertical strut and peephole in the kit based on the detection of EFIRM technologies HPV viruse.
Fig. 3 is the sectional view of Step Shaft and sleeve in the kit based on the detection of EFIRM technologies HPV viruse.
Fig. 4 is the top view of parcel shelf in the kit based on the detection of EFIRM technologies HPV viruse.
In figure:1- reagent box bodies;2- cover boards;3- storage rooms;4- parcel shelf;5- idler wheels;6- driving shafts;7- bottom plates;8-
Driving motor;9- transmission belts;10- bolts;11- screws;12- gears;13- racks;14- monitor stations;15- glass slides;16- colorimetrics
Card;17- Step Shafts;18- sleeves;19- slide bars;The vertical struts of 20-;21- peepholes;22- transverse slats;23- adjusting knobs;24- is regarded
Lens barrel;25- glass plates;26- supporting legs.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other without making creative work
Embodiment belongs to the scope of protection of the invention.
Please refer to Fig.1~4, in the embodiment of the present invention, a kind of kit based on the detection of EFIRM technologies HPV viruse, including
Reagent box body 1;The reagent box body 1 is mainly by storage room 3, driving shaft 6, screw 11, monitor station 14,21 and of peephole
Visor cylinder 24 is formed;1 left side of reagent box body sets sight chamber, and 1 right side of reagent box body sets storage room 3, storage room 3
Hinged closures 2 on the right side of upper end, 2 left end of cover board set bolt 10, and 10 right side of bolt abuts spring, and spring is fixedly connected with cover board 2, and
Two lantern rings of grafting, left end lantern ring are fixedly connected with reagent box body 1 respectively in bolt 10 left side, and right end lantern ring is fixedly connected with cover board 2,
Pass through 10 cover board of bolt, 2 sealed storage chamber 3;2 lower part of cover board sets parcel shelf 4, and parcel shelf 4 is circumferentially uniformly arranged more
A jack, parcel shelf 4 is made for soft rubber material, and 4 both sides of parcel shelf rotation connection idler wheel 5, idler wheel 5 rolls connection storage room
3 inner walls, parcel shelf 4 are clamped storage room 3, and the jack on the parcel shelf 4 of soft rubber material, which can fasten, inserts detected sample examination
Pipe;6 upper end of driving shaft is fixedly connected in the middle part of 4 lower end of parcel shelf, 4 lower part of parcel shelf sets bottom plate 7, and bottom plate 7 is fixedly connected
Driving shaft 6,6 lower end of driving shaft rotation connection driving motor 8, driving motor 5 is fixedly connected with 1 bottom of reagent box body, and drives
Motor 8 is electrically connected power supply and switch, and driving motor 8 can realize positive and negative rotation, 7 support test tube bottom of bottom plate, and driving motor 8 leads to
Electricity work drives driving shaft 6 to rotate and then drives bottom plate 7 and parcel shelf 4 rotates synchronously invisible spectro blood to be detected to storage
It shakes up.
6 lower part of driving shaft rolls connection 9 right end of transmission belt, and 9 left end of transmission belt rolls 11 lower part of connection wire rod, screw
11 bottom bearings rotation connection reagent box body, 1 bottom, 11 top of screw are threadedly coupled Step Shaft 17, and Step Shaft 17 is socketed sleeve
18, and sleeve 18 is fixedly connected with 1 top of reagent box body, 17 outer surface of Step Shaft sets raised line, and 18 inner surface of sleeve sets recessed
Item, so that Step Shaft 17 is only moved up and down along sleeve 18 and cannot rotated under raised line and recessed bar effect;The Step Shaft 17
Upper end is fixedly connected with 22 left end of transverse slat, and 22 right part bearing of transverse slat is rotatablely connected vertical strut 20, and vertical 20 upper end of strut, which is fixed, to be connected
Adjusting knob 23 is connect, vertical strut 20 is socketed reagent box body 1, and vertical 20 lower circumference of strut is layered the peephole that is interspersed
21, peephole 21 is convex lens, and 22 right end of transverse slat is fixedly connected with visor cylinder 24, and 24 lower end of visor cylinder sets glass plate 25, glass
Glass plate 25 is fixedly connected with reagent box body 1, and the driving shaft 6 of rotation drives screw 11 to rotate by transmission belt 9, and screw 11 drives platform
Rank axis 17 moves up and down under sleeve 18 or so and then transverse slat 22 is driven to follow and moves up and down, transverse slat 22 drive vertical strut 20,
Visor cylinder 24 and peephole 21 move up and down, and thickness is gradually reduced peephole 21 from top to bottom, and amplification factor also reduces step by step;Institute
It states 11 lower part of screw and is fixedly connected with gear 12,12 pinion rack 13 of gear, rack 13 is fixedly connected with monitor station 14, and monitor station 14 is right
1 side wall of reagent box body that is slidably connected is held, 14 left upper part of monitor station is fixedly connected with 19 lower end of slide bar, and 19 upper end of slide bar, which is slided, to be connected
Reagent box body 1 is connect, 14 upper surface of monitor station sets glass slide 15, and 15 right side of glass slide sets colorimetric card 16, reagent box body 1
Both sides lower end is fixedly connected with supporting leg 26, and the screw 11 of rotation is rotated with moving gear 12 and then drive rack 13 is moved back and forth so as to band
Dynamic monitor station 14 moves back and forth.
The present invention operation principle be:Switch is pressed when needing detection, and driving motor 8 rotates forward drive driving shaft 6 and rotates
And then bottom plate 7 and parcel shelf 4 is driven to rotate synchronously and shaken up to the invisible spectro blood to be detected of storage, while transmission belt 9 drives
Screw 11 rotates, and screw 11 is rotated with moving gear 12 and then drive rack 13 drives monitor station 14 to release forward, while screw 11
Step Shaft 17 and transverse slat 22 is driven to move up, pulls 10 rotating opening cover board 2 of bolt, after being shaken up using the absorption of rubber head dropper
Blood on test tube, droplet of blood is placed on glass slide 15, reversely presses switch, and driving motor 8 drives parcel shelf 4 to rotate anyway
The blood on other testing tubes to be checked is shaken up again, and screw 11 drives monitor station 14 to return to inside reagent box body 1 anyway, screw
11 band motion plates 22 move down, and eyes are placed on visor cylinder 24, are seen by the peephole 21 on glass plate 25 and vertical strut 20
Survey glass slide 15 on blood and with 16 contrasting detection of colorimetric card, due to peephole 21 be convex lens, and from top to bottom thickness by
Grade reduces, therefore peephole 21 is observed amplification factor and reduced step by step from top to bottom, rotates adjusting knob 23 and drives different sighting distances and thickness
The peephole 21 of degree is directed at glass slide 15 and colorimetric card 16 to observation suitable position.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requirement rather than above description limit, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.Any reference numeral in claim should not be considered as to the involved claim of limitation.
Moreover, it will be appreciated that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
Using specification as an entirety, the technical solutions in each embodiment can also be properly combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (7)
1. a kind of kit based on the detection of EFIRM technologies HPV viruse, including reagent box body(1);The reagent box body
(1)Mainly by storage room(3), driving shaft(6), screw(11), monitor station(14), peephole(21)With visor cylinder(24)Structure
Into, which is characterized in that the reagent box body(1)Right side sets storage room(3), storage room(3)Hinged closures on the right side of upper end
(2), cover board(2)Left end sets bolt(10), bolt(10)Right side abuts spring, and spring is fixedly connected with cover board(2);The cover board
(2)Lower part sets parcel shelf(4), parcel shelf(4)Both sides are rotatablely connected idler wheel(5), idler wheel(5)Roll connection storage room(3)It is interior
Wall, parcel shelf(4)It is clamped storage room(3);The parcel shelf(4)Driving shaft is fixedly connected in the middle part of lower end(6)Upper end, parcel shelf
(4)Lower part sets bottom plate(7), bottom plate(7)It is fixedly connected with driving shaft(6), driving shaft(6)Lower end is rotatablely connected driving motor(8),
Driving motor(5)It is fixedly connected with reagent box body(1)Bottom;The driving shaft(6)Lower part rolls connection transmission belt(9)Right end,
Transmission belt(9)Left end rolls connection wire rod(11)Lower part, screw(11)Bottom bearing is rotatablely connected reagent box body(1)Bottom,
Screw(11)Top is threadedly coupled Step Shaft(17), Step Shaft(17)It is socketed sleeve(18), and sleeve(18)It is fixedly connected with reagent
Box body(1)Top;The Step Shaft(17)Upper end is fixedly connected with transverse slat(22)Left end, transverse slat(22)Right part bearing is rotatablely connected
Vertical strut(20), vertical strut(20)Upper end is fixedly connected with adjusting knob(23), vertical strut(20)It is socketed reagent box body
(1), vertical strut(20)Lower circumference is layered the peephole that is interspersed(21), peephole(21)For convex lens, and transverse slat(22)
Right end is fixedly connected with visor cylinder(24);The screw(11)Lower part is fixedly connected with gear(12), gear(12)Pinion rack(13),
Rack(13)It is fixedly connected with monitor station(14), monitor station(14)Right end is slidably connected reagent box body(1)Side wall, monitor station(14)
Left upper part is fixedly connected with slide bar(19)Lower end, slide bar(19)Upper end is slidably connected reagent box body(1), monitor station(14)Upper table
Face sets glass slide(15), glass slide(15)Right side sets colorimetric card(16).
2. a kind of kit based on the detection of EFIRM technologies HPV viruse according to claim 1, which is characterized in that described
Visor cylinder(24)Lower end sets glass plate(25), glass plate(25)It is fixedly connected with reagent box body(1).
3. a kind of kit based on the detection of EFIRM technologies HPV viruse according to claim 1, which is characterized in that described
Parcel shelf(4)Circumferentially it is uniformly arranged multiple jacks, parcel shelf(4)It is made for soft rubber material.
4. a kind of kit based on the detection of EFIRM technologies HPV viruse according to claim 1, which is characterized in that described
Reagent box body(1)Both sides lower end is fixedly connected with supporting leg(26).
5. a kind of kit based on the detection of EFIRM technologies HPV viruse according to claim 1, which is characterized in that described
Bolt(10)Two lantern rings of grafting are distinguished in left side, and left end lantern ring is fixedly connected with reagent box body(1), right end lantern ring is fixedly connected with lid
Plate(2).
6. a kind of kit based on the detection of EFIRM technologies HPV viruse according to claim 1, which is characterized in that described
Driving motor(8)It is electrically connected power supply and switch.
7. a kind of kit based on the detection of EFIRM technologies HPV viruse according to claim 1, which is characterized in that described
Step Shaft(17)Outer surface sets raised line, sleeve(18)Inner surface sets recessed bar.
Priority Applications (1)
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CN201810002945.5A CN108117979A (en) | 2018-01-02 | 2018-01-02 | A kind of kit based on the detection of EFIRM technologies HPV viruse |
Applications Claiming Priority (1)
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CN201810002945.5A CN108117979A (en) | 2018-01-02 | 2018-01-02 | A kind of kit based on the detection of EFIRM technologies HPV viruse |
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CN108117979A true CN108117979A (en) | 2018-06-05 |
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ID=62233742
Family Applications (1)
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CN201810002945.5A Pending CN108117979A (en) | 2018-01-02 | 2018-01-02 | A kind of kit based on the detection of EFIRM technologies HPV viruse |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110237871A (en) * | 2019-07-11 | 2019-09-17 | 武汉中浩生物技术有限公司 | A kind of stomach Function detection luminescence immunoassay kit |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN110237871A (en) * | 2019-07-11 | 2019-09-17 | 武汉中浩生物技术有限公司 | A kind of stomach Function detection luminescence immunoassay kit |
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