CN108112864A - A kind of method of unprocessed Radix Aconiti Lateralis rapid detoxification - Google Patents
A kind of method of unprocessed Radix Aconiti Lateralis rapid detoxification Download PDFInfo
- Publication number
- CN108112864A CN108112864A CN201810062503.XA CN201810062503A CN108112864A CN 108112864 A CN108112864 A CN 108112864A CN 201810062503 A CN201810062503 A CN 201810062503A CN 108112864 A CN108112864 A CN 108112864A
- Authority
- CN
- China
- Prior art keywords
- radix aconiti
- aconiti lateralis
- fatty acid
- unprocessed radix
- acid compound
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 37
- 238000001784 detoxification Methods 0.000 title claims abstract description 7
- 239000000194 fatty acid Substances 0.000 claims abstract description 29
- 235000014113 dietary fatty acids Nutrition 0.000 claims abstract description 28
- 229930195729 fatty acid Natural products 0.000 claims abstract description 28
- -1 fatty acid compound Chemical class 0.000 claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 20
- 239000000463 material Substances 0.000 claims abstract description 15
- 235000013305 food Nutrition 0.000 claims abstract description 7
- 241000173529 Aconitum napellus Species 0.000 claims abstract 2
- 238000009835 boiling Methods 0.000 claims description 10
- 150000004665 fatty acids Chemical class 0.000 claims description 4
- 238000007654 immersion Methods 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 4
- 238000010411 cooking Methods 0.000 claims 5
- 150000002148 esters Chemical class 0.000 abstract description 14
- 150000002952 aconitine derivatives Chemical class 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 10
- 239000004615 ingredient Substances 0.000 abstract description 7
- 239000004480 active ingredient Substances 0.000 abstract description 6
- 231100000572 poisoning Toxicity 0.000 abstract description 3
- 230000000607 poisoning effect Effects 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 25
- 229930013930 alkaloid Natural products 0.000 description 18
- 239000007921 spray Substances 0.000 description 18
- 238000004458 analytical method Methods 0.000 description 17
- 150000001875 compounds Chemical class 0.000 description 17
- 150000002500 ions Chemical class 0.000 description 15
- 239000000243 solution Substances 0.000 description 15
- 238000002474 experimental method Methods 0.000 description 14
- 241000227129 Aconitum Species 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 10
- 230000002572 peristaltic effect Effects 0.000 description 9
- XFSBVAOIAHNAPC-XTHSEXKGSA-N 16-Ethyl-1alpha,6alpha,19beta-trimethoxy-4-(methoxymethyl)-aconitane-3alpha,8,10alpha,11,18alpha-pentol, 8-acetate 10-benzoate Chemical compound O([C@H]1[C@]2(O)C[C@H]3[C@@]45C6[C@@H]([C@@]([C@H]31)(OC(C)=O)[C@@H](O)[C@@H]2OC)[C@H](OC)[C@@H]4[C@]([C@@H](C[C@@H]5OC)O)(COC)CN6CC)C(=O)C1=CC=CC=C1 XFSBVAOIAHNAPC-XTHSEXKGSA-N 0.000 description 8
- XFSBVAOIAHNAPC-UHFFFAOYSA-N Aconitin Natural products CCN1CC(C(CC2OC)O)(COC)C3C(OC)C(C(C45)(OC(C)=O)C(O)C6OC)C1C32C4CC6(O)C5OC(=O)C1=CC=CC=C1 XFSBVAOIAHNAPC-UHFFFAOYSA-N 0.000 description 8
- 229940039750 aconitine Drugs 0.000 description 8
- STDXGNLCJACLFY-UHFFFAOYSA-N aconitine Natural products CCN1CC2(COC)C(O)CC(O)C34C5CC6(O)C(OC)C(O)C(OC(=O)C)(C5C6OC(=O)c7ccccc7)C(C(OC)C23)C14 STDXGNLCJACLFY-UHFFFAOYSA-N 0.000 description 8
- SQMGCPHFHQGPIF-UUKFDUHUSA-N gns9eex31x Chemical compound O[C@@H]([C@]([C@H]([C@H](O)[C@]12O)OC)(O)C3)[C@H]2[C@@H]3[C@@]2([C@H](C[C@H]3O)OC)[C@H]4[C@@H]1[C@H](OC)[C@@H]2[C@]3(COC)CN4CC SQMGCPHFHQGPIF-UUKFDUHUSA-N 0.000 description 8
- 230000007062 hydrolysis Effects 0.000 description 8
- 238000006460 hydrolysis reaction Methods 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 238000001819 mass spectrum Methods 0.000 description 7
- 241000553739 Aconitum carmichaelii var. truppelianum Species 0.000 description 6
- VKJGBAJNNALVAV-UHFFFAOYSA-M Berberine chloride (TN) Chemical compound [Cl-].C1=C2CC[N+]3=CC4=C(OC)C(OC)=CC=C4C=C3C2=CC2=C1OCO2 VKJGBAJNNALVAV-UHFFFAOYSA-M 0.000 description 6
- 125000003236 benzoyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C(*)=O 0.000 description 6
- 238000010790 dilution Methods 0.000 description 6
- 239000012895 dilution Substances 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- GQRPJUIKGLHLLN-VSTHTWNCSA-N mesaconine Chemical compound COC[C@]12CN(C)C3[C@@H]4[C@H](OC)[C@H]1[C@]3([C@@H]1C[C@@]3(O)[C@H](O)[C@@H]1[C@]4(O)[C@@H](O)[C@@H]3OC)[C@H](C[C@H]2O)OC GQRPJUIKGLHLLN-VSTHTWNCSA-N 0.000 description 6
- 239000012488 sample solution Substances 0.000 description 6
- DPMGVDIWDTYPMP-UHFFFAOYSA-N Hypaconitine Natural products COCC12CCC(OC)C3(CN(C)C1)C4CC5(O)C(OC)C(O)C(CC(OC)C23)(OC(=O)C)C4C5OC(=O)c6ccccc6 DPMGVDIWDTYPMP-UHFFFAOYSA-N 0.000 description 5
- 150000003797 alkaloid derivatives Chemical class 0.000 description 5
- 238000001514 detection method Methods 0.000 description 5
- FIDOCHXHMJHKRW-GKVQVCCJSA-N hypaconitine Chemical compound O([C@H]1[C@]2(O)C[C@H]3[C@]45[C@@H](OC)CC[C@@]6([C@H]4[C@@H](OC)[C@H]([C@@](OC(C)=O)([C@H]31)[C@@H](O)[C@H]2OC)[C@H]5N(C)C6)COC)C(=O)C1=CC=CC=C1 FIDOCHXHMJHKRW-GKVQVCCJSA-N 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 239000003513 alkali Substances 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 238000010438 heat treatment Methods 0.000 description 4
- 239000002184 metal Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 231100000331 toxic Toxicity 0.000 description 4
- 230000002588 toxic effect Effects 0.000 description 4
- 239000003440 toxic substance Substances 0.000 description 4
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 3
- 241000018646 Pinus brutia Species 0.000 description 3
- 235000011613 Pinus brutia Nutrition 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- 230000009021 linear effect Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 231100000614 poison Toxicity 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000001228 spectrum Methods 0.000 description 3
- 238000002137 ultrasound extraction Methods 0.000 description 3
- 239000003643 water by type Substances 0.000 description 3
- WITLAWYGGVAFLU-UHFFFAOYSA-N 3-(6-methoxy-1,3-benzodioxol-5-yl)-8,8-dimethylpyrano[2,3-f]chromen-4-one Chemical compound C1=CC(C)(C)OC2=CC=C(C(C(C3=CC=4OCOC=4C=C3OC)=CO3)=O)C3=C21 WITLAWYGGVAFLU-UHFFFAOYSA-N 0.000 description 2
- DHJXZSFKLJCHLH-BMTFSNIDSA-N 369u7a6hxd Chemical compound O([C@H]1[C@]2(O)C[C@H]3[C@@]45[C@H]6[C@@H]([C@@]([C@H]31)(O)[C@@H](O)[C@@H]2OC)[C@H](OC)[C@@H]4[C@]([C@@H](C[C@@H]5OC)O)(COC)CN6CC)C(=O)C1=CC=CC=C1 DHJXZSFKLJCHLH-BMTFSNIDSA-N 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 241001236235 Pinus halepensis Species 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- DHJXZSFKLJCHLH-UHFFFAOYSA-N benzoylaconine Natural products CCN1CC(C(CC2OC)O)(COC)C3C(OC)C(C(C45)(O)C(O)C6OC)C1C32C4CC6(O)C5OC(=O)C1=CC=CC=C1 DHJXZSFKLJCHLH-UHFFFAOYSA-N 0.000 description 2
- MDFCJNFOINXVSU-CULIVVSNSA-N benzoylhypaconitine Chemical compound C([C@H]1[C@]23[C@@H](OC)CC[C@@]4(C2[C@@H](OC)[C@H]([C@](O)([C@H]11)[C@@H](O)[C@@H]2OC)C3N(C)C4)COC)C2(O)C1OC(=O)C1=CC=CC=C1 MDFCJNFOINXVSU-CULIVVSNSA-N 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 231100000481 chemical toxicant Toxicity 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000006713 insertion reaction Methods 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000010183 spectrum analysis Methods 0.000 description 2
- 239000012086 standard solution Substances 0.000 description 2
- LLEMSCWAKNQHHA-IWXJPZPUSA-N yunaconitine Chemical compound O([C@H]1[C@]2(O)C[C@H]3[C@@]45[C@H]6[C@@H]([C@]([C@H]31)(C[C@@H]2OC)OC(C)=O)[C@H](OC)[C@@H]4[C@]([C@@H](C[C@@H]5OC)O)(COC)CN6CC)C(=O)C1=CC=C(OC)C=C1 LLEMSCWAKNQHHA-IWXJPZPUSA-N 0.000 description 2
- UUHZKVIQJFNQRQ-UHFFFAOYSA-N yunaconitine Natural products CCN1CC2(COC)C(O)CC(OC)C34C5CC6(O)C(O)C5C(CC6OC)(OC(=O)C)C(CC23)C14 UUHZKVIQJFNQRQ-UHFFFAOYSA-N 0.000 description 2
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- 241001269238 Data Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- ZCLSQRQRWNDNLV-UHFFFAOYSA-N acetic acid;dichloromethane;methanol Chemical compound OC.ClCCl.CC(O)=O ZCLSQRQRWNDNLV-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000000889 atomisation Methods 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000000132 electrospray ionisation Methods 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000005040 ion trap Methods 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 238000000622 liquid--liquid extraction Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- CBOSLVQFGANWTL-DVPYZRQCSA-N songorine Chemical compound O=C([C@@H]1C[C@]23[C@@H](C1=C)O)C[C@H]3[C@@]13[C@@H](O)CC[C@@]4(C)CN(CC)[C@@H]1[C@@H]2C[C@H]43 CBOSLVQFGANWTL-DVPYZRQCSA-N 0.000 description 1
- CBOSLVQFGANWTL-UHFFFAOYSA-N songorine Natural products C=C1C(O)C23CC1C(=O)CC3C13C(O)CCC4(C)CN(CC)C1C2CC43 CBOSLVQFGANWTL-UHFFFAOYSA-N 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 235000013547 stew Nutrition 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 230000008673 vomiting Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
- A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
- A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
Landscapes
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Abstract
The invention discloses a kind of methods of unprocessed Radix Aconiti Lateralis rapid detoxification.This method comprises the following steps：Unprocessed Radix Aconiti Lateralis, fatty acid compound and water are boiled altogether.The fatty acid compound can be the food materials rich in fatty acid compound, such as lard.Method provided by the invention can substantially speed up two ester type aconitine compounds containing fast decoupled of monkshood Poisoning ingredient and accelerate the generation of active ingredient monoester type aconitine compounds containing, achieve the purpose that faster " attenuation deposits effect ", simple and effective.
Description
Technical field
The present invention relates to a kind of methods of unprocessed Radix Aconiti Lateralis rapid detoxification.
Background technology
Monkshood is the sub- root of the Ranunculaceae aconitum plant rhizome of Chinese monkshood, and acrid flavour, sweet, property heat are toxic, enter the heart, spleen, kidney channel, tradition
Medicine, which is recorded, has recuperating depleted yang, mends fire supporing yang and cold-dispelling pain-relieving and other effects, is usually used in yang depletion collapse, cold weak pulse of limb, heart-yang is not
The illnesss such as foot, chest impediment and cardialgia, cold of insufficiency type vomiting and diarrhea have in Chinese medicine the good reputation of " the first product of recuperating depleted yang ".Monkshood main product is in Sichuan, lake
The ground such as north, Yunnan, in some areas, the Aconitums medicinal materials such as monkshood decoct it jointly frequently as a kind of nourishing food materials, with chicken etc.
After eat.
Modern pharmacology research shows that active ingredient in monkshood and toxic component are all alkaloids chemical combination therein
Object, wherein the two ester alkaloids such as compounds such as aconitine, Hypaconitine, mesaconine are hypertoxic ingredient, it is necessary before medicinal
Processing processing is carried out, to remove diester-type ingredient therein, 2015 editions《Chinese Pharmacopoeia》Record diester-type in the monkshood after processing
The total amount of alkaloid aconitine, Hypaconitine and mesaconine must not exceed 0.010%.Diester-type aconitine-type alkaloids can lead to
It crosses to decoct for a long time and generates hydrolysis, be progressively hydrolyzed to the smaller monoester alkaloid of toxicity.People will usually stew when edible
Boil 4 it is small when more than detoxify, so still be possible to because two ester alkaloids decompose not exclusively due to generate poisoning.
Based on the situation that hydrolysis removing toxic substances in unprocessed Radix Aconiti Lateralis tradition eating method is slow and less efficient, exploitation one kind can significantly add
The method that its fast toxic component diester-type alkaloids decompose has important practical significance.
The content of the invention
For the situation that hydrolysis removing toxic substances in unprocessed Radix Aconiti Lateralis tradition eating method is slow and less efficient, the present invention provides one kind
New unprocessed Radix Aconiti Lateralis detoxification, that is, son of growing nonparasitically upon another plant with the food materials such as lard rich in fatty acid compound are boiled, can dramatically speeded up altogether
The decomposition of its toxic component diester-type alkaloids, and accelerate the increase of its active ingredient monoester alkaloid, accomplish " to subtract well
Poison deposits effect " purpose.
The detoxification of unprocessed Radix Aconiti Lateralis provided by the present invention, comprises the following steps：By unprocessed Radix Aconiti Lateralis, fatty acid compound and
Water is boiled altogether.
The fatty acid compound can be the food materials rich in fatty acid compound, such as lard.
The addition of the fatty acid compound is more, and the effect of attenuation is better.
When fatty acid compound is lard, the mass ratio of the unprocessed Radix Aconiti Lateralis and lard can be 5g:5ml-5g:50ml,
More specifically can be 5g：20ml.
It can be 1g that the proportioning of water of the unprocessed Radix Aconiti Lateralis with adding in, which is,:5ml-1g:500ml more specifically can be 5g：50mL.
The temperature boiled altogether is 100 DEG C (boilings), and the time boiled altogether can be 120-200min.
The method is additionally included in boil altogether before, the step of unprocessed Radix Aconiti Lateralis are impregnated in the fatty acid compound and water；
The time of the immersion can be 10-30min.
The present invention also provides the new methods that a kind of unprocessed Radix Aconiti Lateralis cook, and comprise the following steps：By unprocessed Radix Aconiti Lateralis, fatty acid
It closes object and water is boiled altogether.
The fatty acid compound can be the food materials rich in fatty acid compound, such as lard.
The addition of the fatty acid compound is more, and the effect of attenuation is better.
When fatty acid compound is lard, the mass ratio of the unprocessed Radix Aconiti Lateralis and lard can be 5g:5ml-5g:50ml,
More specifically can be 5g：20ml.
It can be 1g that the proportioning of water of the unprocessed Radix Aconiti Lateralis with adding in, which is,:5ml-1g:500ml more specifically can be 5g：50mL.
The temperature boiled altogether is 100 DEG C (boilings), and the time boiled altogether can be 120-200min.
The method is additionally included in boil altogether before, the step of unprocessed Radix Aconiti Lateralis are impregnated in the fatty acid compound and water；
The time of the immersion can be 10-30min.
The unprocessed Radix Aconiti Lateralis extracting solution that the above method is prepared falls within protection scope of the present invention.
By comparison it can be found that in decoction process is carried out to unprocessed Radix Aconiti Lateralis, whether lard is added in reaction system, to each
The changes of contents that class compound is particularly two ester type and monoester type aconitine compounds containing has and significantly affects.The addition of lard can
To dramatically speed up the hydrolysis of two ester type aconitine compounds containing of toxic chemical disappearance, while accelerate active ingredient monoester type crow
The generation of head alkaloid compound.After adding lard, two ester alkaloids such as aconitine, mesaconine, Hypaconitine are in reactant
Peak concentration in system reduces about 1/3 or so, is also reduced to Zi appearance to the time completely disappeared from 100 minutes or so 50 minutes
Left and right, significant effect.
Method provided by the invention can substantially speed up two ester type aconitine compounds containing fast decoupled of monkshood Poisoning ingredient
And accelerate the generation of active ingredient monoester type aconitine compounds containing, and achieve the purpose that faster " attenuation deposits effect ", it is simple and effective.
Description of the drawings
Fig. 1 is the Experimental equipment that the present invention uses.
Fig. 2 is EESI ion source structure figures.
Fig. 3 is internal standard Berberine hydrochloride thermal stability curve.
Fig. 4 for monkshood boiling and monkshood boils component in solution changes of contents curve altogether with lard, and (A. Hypaconitines, B. are newly black
Head alkali, C. aconitines, D. benzoyl time aconines, E. benzoylmesaconines, F. benzoyl aconines, the G. rhizome of Chinese monkshood
Former alkali, the new aconines of H., I. aconines, the spirit of J. pine nuts, K. indacontine, L. yunaconitines)
(spirit of A. pine nuts, B. aconine, the new aconines of C., the D. rhizome of Chinese monkshoods are former for each compound mass spectrum two level spectrogram by Fig. 5
Alkali, E. benzoyl time aconines, F. benzoylmesaconines, G. benzoyl aconines, H. Hypaconitines, the I. print rhizome of Chinese monkshoods
Alkali, J. mesaconines, K. aconitines, L. yunaconitines.)
Fig. 6 A are first mass spectrometric figure in lard boiling process；B-F is 5 alkaloids-fatty acid complexes second order ms figure
And structure；G accelerates hydrolysis mechanism for lard.
Specific embodiment
The method of the present invention is illustrated below by specific embodiment, but the present invention is not limited thereto, it is all at this
All any modification, equivalent and improvement done within the spirit and principle of invention etc., should be included in the protection model of the present invention
Within enclosing.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Experiment material and reagent：
Unprocessed Radix Aconiti Lateralis；Berberine hydrochloride (Town in Shanghai spectrum experiment Science and Technology Ltd.)；Yunaconitine (C35H49NO11), print the rhizome of Chinese monkshood
Alkali (C34H47NO10), aconitine (C34H47NO11), mesaconine (C33H45NO11), Hypaconitine (C33H45NO10), benzoyl it is new
Aconine (C31H43NO10), benzoyl Hypaconitine (C31H43NO9), benzoyl aconine (C32H45NO10), aconine
(C25H41NO9), secondary aconine (C24H39NO8), new aconine (C24H39NO9) and songorine (C22H31NO3) etc. 12 standards
Product melt sincere prosperous Deco skill Development Co., Ltd purchased from Beijing；Methanol, dichloromethane (ROE Scientific, Inc., USA)；
Secondary deionized water (laboratory self-control).
Laboratory apparatus：
LTQ-XL Linears ion trap mass spectrometer (LTQ-XL, Finnigan, San Joes, CA)；EESI ion sources (east China
Polytechnics's mass spectrum science and Instrument Laboratory independent development)；Assay balance (METTLER TOLEDO Instrument Ltd.)；Stone
English capillary (ID 0.10mm, OD 0.15mm, Agilent Technologies Co.Ltd., USA)；Micro-sampling pin
(5mL,100μL,Hamiltion,USA)；(BT-100-2J, DG-1 and DG-2 pump head, Baoding Lange constant flow pump are limited for peristaltic pump
Company)；Syringe pump (LSP02-2A, Baoding LanGe constant flow pump Co., Ltd)；(the western instrument science and technology of Shanghai Li Chen nations is limited for electric jacket
Company)；Ultrasonic washing instrument (Kunshan Shu Mei ultrasonic instruments Co., Ltd)；Liquid-transfering gun (the limited public affairs of the silent winged generation that science and technology of U.S.'s match
Department)；0.22 μm of miillpore filter (Tianjin Jin Teng experimental facilities Co., Ltd).
Embodiment
1st, experimental provision
Experimental provision is as shown in Figure 1, provided by the present invention to chemical composition real time on-line monitoring in complex reaction system
The experimental provision of analysis, including reaction generating means, accurate peristaltic pump, electron spray extraction ion source (EESI ion sources) and mass spectrum
Instrument；
The inside of the inlet tube insertion reaction generating means of the precision peristaltic pump, so that in reaction generating means
Sample solution continue precision and pump out, be connected with 0.22 at the port of the inside of the inlet tube insertion reaction generating means
μm miillpore filter, and a metal tube is inserted into the inlet tube at the port；The outlet tube of the precision peristaltic pump goes out
Mouth end connection Y-type three way type, with the accurate peristaltic pump with the outlet of the outlet tube of another accurate peristaltic pump (dilution pumps) of model
End is similarly coupled to the Y-type three way type, and for a variety of solution to be diluted or mixed to sample solution, the Y-type three way type goes out
Sample pipe in mouth connection electron spray extraction ion source, connection mode is to be bridged using metal tube；
The electron spray extraction ion source and mass spectrograph series connection.
It reacts in generating means, it is Chinese medicine decocting device that this experiment is used, using a 250mL three neck round bottom flask.
Because of this requirement of experiment continuous heating, for a long time liquid is kept to seethe with excitement, therefore add a spherical condensation tube, prevent moisture evaporation.Heating
Device is the controllable electric jacket of power, and drag has a magnetic stir bar, ensures that solution system is homogeneous.
Device core position is constant speed precision peristaltic pump, by silica gel hose, can continue precision and pump out in reaction system
Sample solution, the present embodiment decocts reaction system for Chinese medicine, to prevent the solid particulate matters such as the dregs of a decoction from blocking pipeline, takes double
Measure again, i.e. medicinal material are wrapped up using double gauze, and 0.22 μm of miillpore filter is connected at hose inlet.Hose (i.d.0.5mm) is certainly
One section of metal tube (i.d.0.2mm) is inserted into inlet, and there are two effect, the first sample solution that can be reduced in hose is detained,
Make analysis more timely；Second can make hose inlet and filter sink to sample solution, from internal suction sample.Sample pump hose
Outlet connects a Y-type three way type, another side joint model peristaltic pump together, a variety of molten available for being diluted or mixing to sample solution
Liquid.The present embodiment Chinese medicine extract concentration is larger, another pipeline water receiving, adjusts the flow velocity of two peristaltic pumps, and liquid is diluted
10 times of laggard ion source analyses, Y-type three way type outlet connect sample pipe in EESI ion sources, and connection mode is using metal pipe bridge
It connects.
Structure such as Fig. 1 is most for electron spray extraction ion source (Extractive Electrospray Ionization, EESI)
Shown in right side, which is voluntarily developed by East China Institute of Technology's mass spectrum science and Instrument Laboratory.Electron spray extracts
Ionization is in comprehensive electron spray ionisation (ESI) and electron spray desorption ionization (DESI) technological merit and introduces liquid-liquid extraction theory
With the emerging ionization technique invented on the basis of technology.Compared with ESI, the ability of EESI tolerance complex matrices greatly improves, together
When the sensitivity with higher long-time stability, can be gentle molten to liquid, gas under conditions of without sample pretreatment
Glue sample carries out direct ionization, is very suitable for carrying out on-line analysis.
2 experimental methods and result
2.1 sample preparation
Fresh unprocessed Radix Aconiti Lateralis lateral root with main root is separated, removes mud, is cleaned, is cut into slices, is dried at 40 DEG C, obtains raw tag.It is accurate to claim
Dry raw tag 5.00g is taken, 50mL secondary deionized waters are added in conical flask, 30 DEG C of ultrasonic extraction 30min of room temperature are filtered,
Unprocessed Radix Aconiti Lateralis ultrasound Aqueous extracts are obtained, investigated for Mass Spectrometry Conditions optimization and quantitation methodology etc..
The preparation of 2.2 standard solution
12 each 0.00010g of alkaloid standard items accurately are weighed, water is solvent, is configured to 1.0mg/mL standard items mother liquors.
It takes each standard solution appropriate, merges dilution, every 1mL is made to mix in mark solution respectively containing 40 μ g of aconitine, 40 μ g of mesaconine, secondary
100 μ g of aconitine, 100 μ g of benzoyl Hypaconitine, 40 μ g of benzoyl aconine, benzoyl time 40 μ g of aconine, the rhizome of Chinese monkshood
Former 40 μ g of alkali, 100 μ g of secondary aconine, 40 μ g of new aconine, 30 μ g of pine nut spirit, 20 μ g of yunaconitine, 40 μ g of indacontine,
Mark mother liquor must be mixed, is diluted successively with 5 times of volume gradients, obtains a series of hybrid standard product solution.
2.3 Mass Spectrometry Conditions
Electron spray extracts ion source (EESI) structure chart as shown in Fig. 2, by condition optimizing, electron spray capillary in experiment
The angle [alpha] of (or sample spray capillary) between mass spectrum injection port is 150 °, electron spray capillary and sample spray capillary
Between angle beta be 60 °.Electron spray capillary (or sample spray capillary) exports the distance between mass spectrum injection port a
5mm, the distance between electron spray capillary outlet and the outlet of sample spray capillary b are 2mm.Atomization gas uses nitrogen, nitrogen
Pressure is 1.2MPa, spray voltage 3.5KV, 10 μ L/min of electron spray flow velocity, 10 μ L/min of sample flow rate, ion transfer tube temperature
180 DEG C of degree, extractant are methanol dichloromethane-acetate system (50:50:1, v/v/v), other parameter is automatically excellent by system
Change obtains.
Setting LTQ-MS is cation ion detection mode, and scanning range m/z is 300-1200Da.
The drafting of 2.4 standard curves
This experiment uses inner mark method ration, and internal standard compound is Berberine hydrochloride, 1.0 μ g/mL of internal standard concentration, scanning of the mass spectrum pattern
Scanning (SRM) is reacted for selection, the parent ion and detection ion pair information of related compound are shown in Table 1.Using set analysis item
Part is analyzed the mixed mark solution of series, using the ratio at sample peak and internal standard peak as ordinate, with sample concentration than internal standard concentration
Ratio for abscissa, draw standard curve, the results are shown in Table 2.
1 13 compound SRM scanning relevant parameters of table
2.5 methodological study
2.5.1 linear relationship and detection limit, quantitative limit
Using mass spectral analysis condition described in 2.3, the serial hybrid standard product solution prepared by 2.2 is analyzed, with
The ratio of sample peak and internal standard peak intensity is ordinate, using sample concentration and the ratio of internal standard concentration as abscissa, draws standard
Curve, sample concentration when being about respectively 3 and 10 using signal-to-noise ratio (S/N) is detection limit and quantitative limit, 12 alkaloids chemical combination
The relevant parameter of object is shown in Table 2.
Mark song, linear relationship, detection limit and the quantitative limit of 2 12 alkaloid compounds of table
2.5.2 repeatability and precision are investigated
Monkshood ultrasonic extraction liquid is taken to do method repeatability to investigate, extracting solution dilutes 100 times, and internal standard is added to make 1 μ g/ of internal standard concentration
ML, it is parallel to be repeated 6 times using the 2.3 mass spectral analysis conditions, the relative standard deviation (RSD) of each compound measured value is calculated,
Investigate analysis method repeatability.
Precision investigate using high A, in, the hybrid standard product solution of low three concentration as sample, the parallel sample preparation of each concentration
Analysis 6 times calculates the RSD of each compound analysis result, investigates the precision of analysis method.Correlated results is shown in Table 3.
3 repeatability of table and precision are investigated
2.5.3 internal standard thermal stability is investigated
Yin Ben researchs are the on-line checking of ingredient during decocting for Chinese herbal medicine, system are heated in experiment and continue to boil
It rises, therefore the thermal stability of internal standard compound need to be investigated.The Berberine hydrochloride aqueous solution of 1 μ g/mL is prepared, adds in 1/5 body
Product lard, heating take heat 0h, 0.5h, 1h respectively, 2h, 3h, and the sample after 4h is analyzed, and observes internal standard compound concentration
Whether stablize.The results are shown in Figure 3 for it, it is seen that internal standard jamaicin heats content in oil mixing with water system and keeps stablizing.
2.5.4 matrix effect is investigated
Influence of the complex matrices dissolved out in decoction process to target component assay is investigated, takes medicine obtained by ultrasonic extraction
Liquid dilute 100 times, it is parallel preparation three groups of samples, sample A be liquid+water, sample B be liquid+hybrid standard product solution, sample C
For hybrid standard product solution+water, mixed mark solution sets high, medium and low three concentration to investigate respectively, the analysis method point as shown in 2.3
Analysis, each compound concentration value of gained substitute into formula：Parallel analysis 6 times calculates average and RSD, the results are shown in Table 4.
4 matrix effect of table is investigated
2.6 monkshood boilings and boil procedure component mutation analysis altogether with lard
According to the above-mentioned analysis method for having been established and investigating, solution chemistry during being boiled altogether to raw tag boiling and with lard
The variation of ingredient carries out qualitative and quantitative analysis.
Boiling experiment gives birth to tag 5g using medicinal material, adds in 50mL secondary deionized waters, 10 μ g/ of internal standard (Berberine hydrochloride)
ML, using the on-line analysis device built, medicinal material is begun to warm up after impregnating 30min in flask, heating power 125W, and sample is compacted
1 μ L/min of dynamic pump rate, 9 μ L/min of dilution pump rate, into EESI ion sources after 10 times of dilutions, are analyzed in real time.
It boils experiment altogether with lard and tag 5g is given birth to using medicinal material, add in 50mL secondary deionized waters, 20mL lards add internal standard
(Berberine hydrochloride) 10 μ g/mL, using the on-line analysis device built, medicinal material begins to warm up after impregnating 30min in flask, adds
Thermal power 125W, 1 μ L/min of sample wriggling pump rate, 9 μ L/min of dilution pump rate, enter EESI ion sources after 10 times of dilutions,
Analysis in real time.
Boiling experiment, with lard boil altogether experiment in each component content change curve as shown in figure 4, each area under the curve
(AUC) 5 are shown in Table.Each compound characterization identification is the matter of related compound by being completed with standard items to CID crumb datas
Compose two level spectrogram as shown in Figure 5.
5 each compounds content area under the curve of table
Note：- for significantly, * is not notable, and * * is extremely significantly.
With reference to Fig. 4 and table 5, it can be found that in decoction process is carried out to monkshood, lard whether is added in reaction system, it is right
The changes of contents that classes of compounds is particularly two ester type and monoester type aconitine compounds containing has and significantly affects.The addition of lard
The hydrolysis that two ester type aconitine compounds containing of toxic chemical can be dramatically speeded up disappears, while accelerates active ingredient monoester type
The generation of aconitine compounds containing.After adding lard, two ester alkaloids such as aconitine, mesaconine, Hypaconitine are reacting
Peak concentration in system reduces about 1/3 or so, and also 50 points were reduced to from 100 minutes or so to the time completely disappeared Zi appearance
Clock or so, significant effect.For the changes of contents of the other compositions such as pine nut spirit, the addition of lard does not make significant difference to it.Eachization
It closes object area under the curve T inspection results to show, in addition to pine nut spirit, lard cooks each compound area under the curve compared with pure water boils
All there is significant difference.
It was found that the fatty acid ingredient in lard may be by that ester occurs first with two ester type aconitines in experiment
Then exchange reaction is further being hydrolyzed to monoester type aconitine, which is carried out at the same time with two ester type direct hydrolysis, is two
Ester alkaloid adds a reaction path to monoester alkaloid, therefore can dramatically speed up hydrolysis and the monoesters of two ester type
The generation of type.
The level-one spectrum of solution composition when Fig. 6 is about 56-58min in lard boiling process, it can be seen that in m/z 900 or so
There are a series of small peaks, it is right by document, the identification of CID two levels spectrum, 5 alkaloids and fat have been carried out to which part peak
The compound qualification result of fat acid is shown in Table 6, our suppositions to reaction mechanism of tentative confirmation.
6 partial fatty acids of table-alkaloid composite qualification result
Claims (10)
1. a kind of method of unprocessed Radix Aconiti Lateralis rapid detoxification, comprises the following steps：Unprocessed Radix Aconiti Lateralis, fatty acid compound and water are total to
It boils.
2. according to the method described in claim 1, it is characterized in that：The fatty acid compound is rich in fatty acid chemical combination
The food materials of object.
3. according to the method described in claim 2, it is characterized in that：The fatty acid compound is lard；The unprocessed Radix Aconiti Lateralis
Mass ratio with lard is 5g:5ml-5g:50ml.
4. according to claim 1-3 any one of them methods, it is characterised in that：The unprocessed Radix Aconiti Lateralis and the proportioning of water added in are
1g:5ml-1g:500ml；
The time boiled altogether is 120-200min.
5. according to claim 1-4 any one of them methods, it is characterised in that：The method is additionally included in boil altogether before, by life
The step of monkshood impregnates in the mixed liquor of the fatty acid compound and water；The time of the immersion is 10-30min.
6. a kind of method for cooking of unprocessed Radix Aconiti Lateralis, comprises the following steps：Unprocessed Radix Aconiti Lateralis, fatty acid compound and water are boiled altogether.
7. the method for cooking of unprocessed Radix Aconiti Lateralis according to claim 6, it is characterised in that：The fatty acid compound is to be rich in
The food materials of fatty acid compound.
8. the method for cooking of unprocessed Radix Aconiti Lateralis according to claim 7, it is characterised in that：The fatty acid compound is pig
Oil；The mass ratio of the unprocessed Radix Aconiti Lateralis and lard is 5g:5ml-5g:50ml；
The proportioning of water of the unprocessed Radix Aconiti Lateralis with adding in is 1g:5ml-1g:500ml；
The time boiled altogether is 120-200min.
9. according to the method for cooking of claim 6-8 any one of them unprocessed Radix Aconiti Lateralis, it is characterised in that：The method is additionally included in
Before boiling altogether, the step of unprocessed Radix Aconiti Lateralis are impregnated in the fatty acid compound and water；The time of the immersion is 10-30min.
10. the unprocessed Radix Aconiti Lateralis extracting solution that the method for cooking of unprocessed Radix Aconiti Lateralis any one of claim 6-9 is prepared.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810062503.XA CN108112864A (en) | 2018-01-23 | 2018-01-23 | A kind of method of unprocessed Radix Aconiti Lateralis rapid detoxification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810062503.XA CN108112864A (en) | 2018-01-23 | 2018-01-23 | A kind of method of unprocessed Radix Aconiti Lateralis rapid detoxification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108112864A true CN108112864A (en) | 2018-06-05 |
Family
ID=62233182
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810062503.XA Pending CN108112864A (en) | 2018-01-23 | 2018-01-23 | A kind of method of unprocessed Radix Aconiti Lateralis rapid detoxification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108112864A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116270818A (en) * | 2023-02-10 | 2023-06-23 | 宁波市疾病预防控制中心 | Attenuation method of diester diterpenoid alkaloids in aconite |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580047A (en) * | 2004-05-20 | 2005-02-16 | 中国科学院长春应用化学研究所 | Aliphatic ester aconite alkaloid synthesizing method |
| CN1903261A (en) * | 2006-08-11 | 2007-01-31 | 中国科学院长春应用化学研究所 | Method for processing monkshood |
| CN101390963A (en) * | 2007-09-17 | 2009-03-25 | 廖德宏 | Process method of aconite |
| CN101591294A (en) * | 2009-07-02 | 2009-12-02 | 中国科学院长春应用化学研究所 | Aconite diester-type alkaloids is converted into the method for ester-type alkaloids |
| CN102949472A (en) * | 2012-10-24 | 2013-03-06 | 宝鸡向源中药饮片有限责任公司 | Dynamic circulating processing method of prepared radix aconiti and radix aconiti kusnezoffii traditional Chinese medicine decoction piece |
-
2018
- 2018-01-23 CN CN201810062503.XA patent/CN108112864A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1580047A (en) * | 2004-05-20 | 2005-02-16 | 中国科学院长春应用化学研究所 | Aliphatic ester aconite alkaloid synthesizing method |
| CN1903261A (en) * | 2006-08-11 | 2007-01-31 | 中国科学院长春应用化学研究所 | Method for processing monkshood |
| CN101390963A (en) * | 2007-09-17 | 2009-03-25 | 廖德宏 | Process method of aconite |
| CN101591294A (en) * | 2009-07-02 | 2009-12-02 | 中国科学院长春应用化学研究所 | Aconite diester-type alkaloids is converted into the method for ester-type alkaloids |
| CN102949472A (en) * | 2012-10-24 | 2013-03-06 | 宝鸡向源中药饮片有限责任公司 | Dynamic circulating processing method of prepared radix aconiti and radix aconiti kusnezoffii traditional Chinese medicine decoction piece |
Non-Patent Citations (2)
| Title |
|---|
| 吕志杰等: "《仲景方药古今应用》", 31 August 2000, 中医古籍出版社 * |
| 蔡宝昌等: "中药炮制过程化学机理研究", 《化学进展》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116270818A (en) * | 2023-02-10 | 2023-06-23 | 宁波市疾病预防控制中心 | Attenuation method of diester diterpenoid alkaloids in aconite |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Xia et al. | 8-Hydroxyquinoline–chloroform single drop microextraction and electrothermal vaporization ICP-MS for the fractionation of aluminium in natural waters and drinks | |
| CN104697983B (en) | The detection method of heavy metal lead, cadmium, arsenic, copper in a kind of prepared slices of Chinese crude drugs | |
| CN102429966B (en) | A kind of concocting method without gallbladder Radix Aconiti Lateralis Preparata | |
| CN108008026B (en) | Method for synchronously detecting 13 colorants in hawthorn pills | |
| CN104991009A (en) | Method for determination of illegally added substances in traditional Chinese medicines and health-care products | |
| CN106645450A (en) | Quality detection method for novel biological particle | |
| CN108414608A (en) | A kind of method and its dedicated unit to chemical composition real time on-line monitoring analysis in complex reaction system | |
| CN110585385B (en) | Extraction method and qualitative analysis method of alpinia oxyphylla sesquiterpenoids | |
| CN109406682A (en) | The UPLC characteristic spectrum construction method and detection method of ginger medicinal material | |
| CN108112864A (en) | A kind of method of unprocessed Radix Aconiti Lateralis rapid detoxification | |
| Lin et al. | Application of LC/MS and ICP/MS for establishing the fingerprint spectrum of the traditional Chinese medicinal preparation Gan‐Lu‐Yin | |
| Mak et al. | The characterization of steam distillation as an extraction method to extract volatile compounds from Prunella vulgaris and the investigation of their anti-tumorous effect | |
| CN111413443A (en) | Characteristic spectrum, construction method and identification method of angelica sinensis and decoction pieces thereof | |
| CN113484429B (en) | Method for establishing standard of peach pit qi-bearing soup material | |
| Sun et al. | Investigation of distribution for trace Lead and Cadmium in chinese herbal medicines and their decoctions by graphite furnace Atomic Absorption Spectrometry | |
| CN105806964A (en) | Method for detecting Acanthopanax senticosus and Glycyrrhiza uralensis preparation and application thereof | |
| CN115932145A (en) | Method for detecting oligosaccharide in compound donkey-hide gelatin syrup and construction of oligosaccharide UPLC-MS contrast fingerprint spectrum of compound donkey-hide gelatin syrup | |
| Gu et al. | Rapid quality evaluation of Chinese herbal medicines using a miniature mass spectrometer: Lygodium japonicum (Thunb.) Sw. as an example | |
| CN106706635A (en) | Quality standard of dogbane leaf qualitative and quantitative traditional Chinese medicine decoction piece and manufacturing process thereof | |
| Xu et al. | Qualitative and quantitative determination of nine main active constituents in Pulsatilla cernua by high‐performance liquid chromatography coupled to electrospray ionization tandem mass spectrometry | |
| CN113368185A (en) | Preparation process and quality control method of lily and rehmannia soup substance standard | |
| CN114796347B (en) | Preparation method and detection method of prepared rehmannia root | |
| CN114720602B (en) | Method for constructing characteristic spectrum of lithospermum through medicinal material or standard decoction thereof and detection method | |
| CN113484428B (en) | Construction method of peach pit qi-bearing decoction characteristic spectrum | |
| CN113759026B (en) | Common clubmoss herb and preparation characteristic map and construction method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |