CN108103598A - A kind of electrostatic spinning reception device for preparing axial orientation tubular tissue engineering material - Google Patents
A kind of electrostatic spinning reception device for preparing axial orientation tubular tissue engineering material Download PDFInfo
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- CN108103598A CN108103598A CN201810132897.1A CN201810132897A CN108103598A CN 108103598 A CN108103598 A CN 108103598A CN 201810132897 A CN201810132897 A CN 201810132897A CN 108103598 A CN108103598 A CN 108103598A
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- Prior art keywords
- spool
- reception device
- electrostatic spinning
- tissue engineering
- axial orientation
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Classifications
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
Abstract
A kind of electrostatic spinning reception device for preparing axial orientation tubular tissue engineering material, belong to tissue engineering material preparation field, including electrostatic spinning spinning nozzle, reception device is equipped with below spinning nozzle, reception device includes reception pole plate and spool a pair of vertically arranged and be mutually parallel, and spool receives pole plate perpendicular to two and sets, takes the upper edge for being placed on two reception pole plates；Reception device further includes the propulsive mechanism that spool is promoted to do back and forth movement along the horizontal direction perpendicular to spool；Rotating mechanism is also associated on spool.Fiber axial orientation degree in the nano-fiber tubular tissue engineering material prepared using the device is high, and being conducive to endothelial cell axial orientation as artificial blood vessel grows and form the endothelial tissue as real blood vessels.
Description
Technical field
The invention belongs to tissue engineering material preparation fields, and in particular to a kind of to prepare the tubulose with the structure being axially oriented
The electrostatic spinning reception device of tissue engineering material.
Background technology
Angiocardiopathy serious threat the health of the mankind, is clinically mainly made at present using interventional treatment and vascular bypass surgery
For treatment means；Since interventional treatment cannot avoid the generation of restenosis completely, vascular bypass surgery is still optimal selection, but often
It cannot be implemented due to the vascular lesion of autologous vein source deficiency or some complexity.Therefore, research and development can substitute autologous vein
Artificial blood vessel or tubular tissue engineering rack be increasingly becoming the hot spot of research.
Electrostatic spinning technique was developed rapidly in more than ten years in past, be prepare nanofiber effective way it
One；The nanofiber pipe holder prepared by method of electrostatic spinning has the characteristics that high porosity, small-bore and high-specific surface area, has
Huge application potential.
In general, electrospinning fibre deposits the flourish on the reception device, being unfavorable for cell in the form of random, this limitation
Its application in intravascular tissue engineering.Therefore, have scholar to the orientations of fiber in electrospinning tubular blood vessel support into
Gone research, the method for most common of which be using the reception shaft of rotation, first so that fiber is deposited on shaft surface vertically,
The intravascular stent of tubulose is formed by the rotation of shaft again.Although this method can effectively obtain fiber along shaft axial orientation
The tubular blood vessel support of arrangement, but with the continuous progress of spinning process, tubular bracket thickness increases therewith, insulating bar and metal
Electrical potential difference between block or magnetic pole system reduces, and the axial orientation degree of fiber can be also gradually reduced, thus not ensure that tubulose
Intravascular stent is also what axial orientation arranged compared with the fiber of outer layer.After spinning, how nanofiber pipe to be taken from shaft
Down and the problem that is faced of those skilled in the art.In addition, using prior art engineering blood vessel, also there are fiber fractionations
The problem of cannot solve.
The content of the invention
The purpose of the present invention is intended to provide a kind of electrostatic spinning for the tubular tissue engineering material for being used to prepare axial orientation
Reception device.
To achieve the above object, this invention takes following technical solutions：It is a kind of to prepare axial orientation tubular tissue engineering
The electrostatic spinning reception device of material including electrostatic spinning spinning nozzle, is equipped with reception device, reception device includes below spinning nozzle
Reception pole plate that is a pair of vertically arranged and being mutually parallel, reception device further include spool, and spool receives pole plate perpendicular to two and sets
It puts, take the upper edge for being placed on two reception pole plates；Reception device further include promote spool along perpendicular to spool horizontal direction do it is past
Return the propulsive mechanism of movement；Rotating mechanism is also associated on spool.It should between spinning nozzle and reception pole plate in electrostatic spinning technique
With appropriate electrical potential difference, circuit connection can be that spinning nozzle is connected with cathode, receives pole plate and be connected or spray with anode
Silk mouth is connected with anode, receives pole plate and be connected cathode or ground connection.Receive the dielectric material that pole plate generally selects dielectric constant to be more than 3
It is made.
The distance of spinning nozzle to reception pole plate is 140~200mm；It is 30~60mm that two, which receive the distance between pole plate,.
Spool does the cycle of back and forth movement as 5~20s, and effective travel is 200~600mm, and the movement linear velocity of spool is
20~60mm/s；The angular velocity of rotation of spool is 1~10rad/s.
Propulsive mechanism includes being horizontally placed on the screw received below pole plate perpendicular to spool；Slide unit is bolted on screw,
Slide unit is equipped with pedestal, and the spool support seat positioned at spool both ends is connected on pedestal.
Rotating mechanism, which includes being arranged on, receives the pole plate both sides rack parallel with receiving pole plate, and spool is equipped with to match with rack
The gear of conjunction.Gear radius is preferably controlled in 5mm~10mm.
The positive and negative motor that propulsive mechanism further includes control system and is connected with screw；Control system include controller and with
The driver that controller is connected, driver are equipped with power supply, and driver is connected with positive and negative motor.
Spool is externally provided with water-soluble polymer coating.
Water-soluble polymer is selected from PEG（Polyethylene glycol）、PEO（Polyethylene glycol oxide）Or PVA（Polyvinyl alcohol）.
The spinning solution that electrostatic spinning uses is fibroin albumen, collagen, l-lactic acid, polycaprolactone, left-handed poly- breast
The solution of one or more kinds of polymer in sour polycaprolactone co-polymer.Concentration of dope general control for 6~16w/v%,
Solvent is selected from dichloromethane, hexafluoroisopropanol, dimethylformamide and chloroform.
Spool by plater coat water-soluble polymer, the plater include upper mold and with upper mold it is matched under
Mould, the lower surface of upper mold and the upper surface of lower film enclose spool accommodating chamber, and spool accommodating chamber includes being arranged at pouring for stage casing
Chamber and the limit chamber for being arranged at both ends, the internal diameter of limit chamber are equal to spool outer diameter but less than intracavitary footpath is poured, in other words spool
Accommodating chamber is less than the step column of stage casing diameter in both ends diameter；It is additionally provided on plater and is poured with pour that chamber connects
Hole.
Tissue engineering material is prepared using reception device provided by the invention, electrostatic spinning process parameter is can be controlled in：It pushes away
It is 1~2.5ml/h to promote rate into device, and electrostatic high-pressure is 10~15kV.In spinning process, from the electrospinning fibre of spinning nozzle ejection
It dropping to and receives at the top of pole plate, be overlapped on two and receive on pole plate between, spool, which is equally also taken to be placed on, receives edge on pole plate,
It receives at the top of pole plate along rotation while doing back and forth movement perpendicular to spool direction, will receive and receive electrospinning fibre at the top of pole plate
Roll and be wound up into spool, after being gradually accumulate to certain thickness on spool along the fiber of spool axial orientation substantially, formation group
Weaver's journey nanofiber pipe.Fiber axial orientation degree in the nanofiber pipe is high, and it is thin to be conducive to endothelium as artificial blood vessel
Born of the same parents' axial orientation grows and forms the endothelial tissue as real blood vessels.
In addition, beneficial effects of the present invention are also embodied in：
（1）The distance for controlling spinning nozzle to reception pole plate is 140~200mm, is because distance is too near, and solvent has little time to volatilize,
Fiber also has little time the fibre pipe limited length elongated, prepared, and fibre diameter is big, is easily mutually bonded between fiber, should
It is little with value；Distance is too big, and electric field strength dies down, then fiber is difficult to be collected.Because in the reception distance of 140~200mm
Interior, it is 30~60mm to control two reception the distance between pole plates, and fiber can be made to ride over two just and received on pole plates again not
As for going out the fiber that hangs too much more than making on the outside of reception pole plate.
（2）It is received limiting on the basis of, the depression area of electrospinning fibre is controlled, and spool only need to be under spinning nozzle
Back and forth movement is done in the range of 200~600mm of side.Spool is controlled to do the cycle of back and forth movement as 5~20s, the fortune of spool
Moving-wire speed is 20~60mm/s, and the angular velocity of rotation of spool is 1~10rad/s, and the angular speed of spool should be with its linear velocity
Matching, if angular speed does not catch up with linear velocity, it is easy to pull apart to influence the physical property of finished product by fiber or even influence fiber
The degree of orientation.It in addition, is wound when electrospinning fibre just lands, it is easy to the fiber being wound up on spool
It is adhered to each other, forms the one of more fastness.If spool movement is excessively slow, fiber has been piled into thicker at the top of reception pole plate
Layer, interlayer is easily layered after coiling and molding.But if the linear velocity of spool is too fast, there may not be not also electrospinning fibre to land or drop
Fall that amount is few, the winding efficiency of spool is low.
（3）Water-soluble polymer coating is coated in spool surface, it is then recycled to remove winding fibroin albumen, collagen egg
In vain, the electrospinning fibre that the polymer such as l-lactic acid, polycaprolactone, l-lactic acid polycaprolactone co-polymer are formed, because water-soluble
Property polymer there is good damp effect, be easy to remove electro spinning nano fiber pipe from spool after the completion of winding, can
Production efficiency is substantially improved.
（4）Using screw spool is promoted to do linear movement, gear is installed on spool, and receive pole plate both sides set with
The parallel rack of pole plate is received, wheel and rack engagement, spool is while linear movement is done under the action of gear and rack
It rotates, the pass between spool point-to-point speed and rotational angular velocity can be easily controlled by the diameter for changing gear
System.Control system and the positive and negative motor that is connected with screw be further set, control system include controller and with controller phase
The driver of connection, driver is equipped with power supply, and is connected with positive and negative motor, by the rotation of controller control point motor, makes
The preparation of nanofiber pipe efficiently carries out in order.
（5）Further water-soluble polymer coating is assigned in spool surface using the mode that mold pours, thickness is controllable,
Surface is regular, and the coating of formation is more uniform.
Description of the drawings
Fig. 1 is the front view of reception device in embodiment 1；
Fig. 2 is the top view of reception device in embodiment 1；
Fig. 3 is propulsive mechanism schematic diagram in embodiment 1；
Fig. 4 is 1 floating coat schematic device of embodiment；
Fig. 5 is the principle of the present invention figure；
Fig. 6 is the digital photograph of the spool with PEG coatings；
Fig. 7 is the digital photograph of nanofiber pipe sample made from embodiment 1；
Fig. 8 is the scanning electron microscope (SEM) photograph of nanofiber pipe sample made from embodiment 1；
Fig. 9 is the scanning electron microscope (SEM) photograph of nanofiber pipe sample made from embodiment 1；
Figure 10 is the scanning electron microscope (SEM) photograph of control sample in embodiment 1；
In figure：1- controllers, 2- drivers, 3- power supplys, the positive and negative motors of 4-, 5- slide units, 6- screws, 7- support plates, 8- gears, 9-
Rack, 10- reception pole plates, 11- pedestals, 12- support bases, 13- spools, 14- stents, 15- upper molds, 16- lower dies, 17- pouring holes,
18- pours chamber, 19- limit chambers, 20- spinning nozzles.
Specific embodiment
With reference to specific embodiment, the present invention will be further described.
Embodiment 1
A kind of electrostatic spinning reception device for preparing axial orientation tubular tissue engineering material, as shown in Figs 1-4, including promoting
Device, propeller are connected with spinning nozzle 20, and electrostatic high-pressure bringing device is equipped at spinning nozzle 20, and 20 syringe needle of spinning nozzle connection electrostatic is high
The anode of pressure load device, 20 lower section of spinning nozzle are equipped with reception device.Reception device includes a pair of vertically arranged and is mutually parallel
Reception pole plate 10 and spool 13, receive cathode and ground connection that pole plate 10 connects electrostatic high-pressure bringing device, receive pole plate 10
Both ends are installed on the support 14, and spool 13 receives pole plate 10 perpendicular to two and sets, takes the upper edge for being placed on two reception pole plates 10.
Two receive the distance between pole plate 10 as 40mm, and spinning nozzle 20 to the distance for receiving pole plate 10 is 180mm.
Reception device further includes the propulsive mechanism that spool 13 is promoted to do back and forth movement along the horizontal direction perpendicular to spool 13：
Propulsive mechanism includes being horizontally placed on the screw 6 for receiving 10 lower section of pole plate perpendicular to spool 13；Slide unit 5 is bolted on screw 6, it is sliding
Platform 5 is equipped with pedestal 11, and the support base 12 positioned at 13 both ends of spool is connected on pedestal 11, and 13 both ends of spool are mounted on support base
On 12.5 lower part of slide unit is equipped with sliding block, and the lower section of slide unit 5 is equipped with support plate 7, and support plate 7 is equipped with slide, slide unit 5 by sliding block,
Slide is slidably connected with support plate 7；It installs on the support 14 at 7 both ends of support plate.Propulsive mechanism further include control system and with silk
The positive and negative motor 4 that bar 6 is connected；Control system includes controller 1 and the driver 2 being connected with controller 1, and driver 2 is set
There is power supply 3, driver 2 is connected with positive and negative motor 4.
Rotating mechanism is also associated on spool 13：Rotating mechanism includes being arranged on reception 10 both sides of pole plate and reception pole plate 10 is flat
Capable rack 9, spool 13 are equipped with and 9 matched gear 8 of rack.
The effective travel control of spool 13 is in 200mm, and period of motion of spool 13 is 10s, the movement linear velocity of spool 13
For 40mm/s；8 radius of gear is 8mm, and the angular velocity of rotation of spool 13 is 5rad/s.
Spool 13 is outer to coat PEG coatings by following plater：Plater includes upper mold 15 and matches with upper mold 15
The lower die 16 of conjunction, the lower surface of upper mold 15 and the upper surface of lower film 16 enclose spool accommodating chamber, spool accommodating chamber bag after molding
Include and be arranged at pouring chamber 18 and being arranged at the limit chamber 19 at both ends for stage casing, the internal diameter of limit chamber 19 be equal to the outer diameter of spool 13 but
Less than the internal diameter for pouring chamber 18.Upper mold 15 is fastened by bolts after being molded with lower die 16.Chamber is additionally provided with and poured on plater
The pouring hole 17 of 18 connections.
Electrostatic spinning is carried out using above device, prepares the tissue engineering material of axial orientation, the course of work is as follows：
（1）Make coating：Spool 13 is put into the spool accommodating chamber in lower die 16, spool 13 is carried out by limit chamber 19
/V；Upper mold 15 and lower die 16 are molded, fastened upper mold 15 and lower die 16 using bolt.By prepared PEG aqueous solutions edge
Pouring hole 17, which pours into, to be poured in chamber 18, and so as to coat PEG aqueous solutions on spool 13, PEG aqueous solutions form water through naturally dry
Soluble polymer coating（Damp coating）.Bolt is screwed out, coated spool 13 is taken out to spare, digital photograph such as Fig. 6 institutes
Show.
（2）Prepare spinning solution：Using dichloromethane and dimethylformamide as solvent, compound concentration is the left-handed of 7.5w/v%
Polylactic acid poly caprolactone spinning solution.
（3）Electrostatic spinning：By step（1）Spool 13 obtained is installed on support base 12, by step（2）Prepared spinning
Silk solution is added in propeller, and the propulsion rate of propeller is set to start spool 13 for 1.5ml/h, electrostatic high-pressure 14kV
Propulsive mechanism and rotating mechanism, proceed by electrostatic spinning.
As shown in figure 5, spinning solution is transported to spinning nozzle 20 and is sprayed downwards from spinning nozzle 20, the polymerization of ejection by propeller
Object solution is cleaved under the action of high voltage electric field into fiber, and to back and forth folding landing at reception device, every section form fold it is single
The fiber of member falls to receive 10 top of pole plate at two, rides over two and receives on pole plate 10 between.At the same time, controller 1 is made
With controller 1 sends positive rotaring signal to driver 2, and driver 2 receives positive rotaring signal；Driver 2 sends positive rotaring signal to positive and negative
Motor 4, positive and negative motor 4 rotate forward, and screw 6 is driven to rotate；Screw 6 applies slide unit 5 active force, and slide unit 5 is positive mobile, drives volume
Axis 13 and the gear 8 on spool 13 are moved together with slide unit 5.Gear 8 is acted on rack 9 so as to rotate, into one
Step drives spool 13 to rotate together.Spool 13 does linear movement while rotating, and will be overlapped on the orientation on reception pole plate 10
Fiber is outside spool 13.
After 5s, controller 1 sends reverse signal to driver 2, and driver 2 receives reverse signal；Driver 2 sends reversion
Signal to positive and negative motor 4, positive and negative motor 4 inverts；Screw 6 rotates, and screw 6 applies active force to slide unit 5, and slide unit 5 moves backward,
Spool 13 and gear 8 are moved together with slide unit 5；Gear 8 is acted on by rack 9, and gear 8 drives spool 13 to rotate together；Spool 13
Continue to be wound the orientation fiber being overlapped on reception pole plate 10.And then positive and negative motor 4, which once rotates forward, once to be inverted, volume
Axis 13 completes a period of motion.
Above-mentioned action is repeated, fiber is gradually built into pipe outside spool 13, forms the nanofiber with axial orientation structure
Tubing engineering material, the digital photograph of gained sample are as shown in Figure 7.It is bright and clean that the sample surfaces are can be seen that from Fig. 7 photos,
End face is solid, and no lamination occurs.
Electron microscope experiment is scanned to sample made from the present embodiment, experimental result is as shown in Figure 8 and Figure 9.From Fig. 8 samples
The fiber that the scanning electron microscope (SEM) photograph on surface can be seen that in the sample is axially oriented substantially；From the scanning electricity of Fig. 9 example cross sections
Mirror figure accumulates close, no layering between can be seen that fiber.
With reference to the present embodiment, spool 13 is set to do the cycle of back and forth movement as 40s, prepares control sample, control sample is swept
It is as shown in Figure 10 to retouch electron microscope, from Figure 10, it is apparent that more serious lamination has occurred in control sample.
Embodiment 2
A kind of electrostatic spinning reception device for preparing axial orientation tubular tissue engineering material, structure is same as Example 1, different
Part is：It is 30mm that two, which receive the distance between pole plate 10,；The distance of spinning nozzle 20 to reception pole plate 10 is 140mm.Volume
Axis 13 does the cycle of back and forth movement as 5s, effective travel 150mm, and the movement linear velocity of spool 13 is 60mm/s；Spool 13
Angular velocity of rotation is 10rad/s.
13 surface of spool coats PEO（Polyethylene glycol oxide）.
Spinning solution is using dichloromethane and dimethylformamide as the polycaprolactone solution of solvent, concentration 16w/v%.
Electrostatic spinning process parameter setting is as follows：Propeller promotes rate 2.5ml/h, electrostatic high-pressure 10kV.
Embodiment 3
A kind of electrostatic spinning reception device for preparing axial orientation tubular tissue engineering material, structure is same as Example 1, different
Part is：It is 60mm that two, which receive the distance between pole plate 10,；The distance of spinning nozzle 20 to reception pole plate 10 is 200mm.Volume
Axis 13 does the cycle of back and forth movement as 20s, effective travel 400mm, and the movement linear velocity of spool 13 is 20mm/s；Spool 13
Angular velocity of rotation is 1rad/s.
13 surface of spool coats PVA（Polyvinyl alcohol）.
Spinning solution is using dichloromethane and dimethylformamide as the l-lactic acid solution of solvent, concentration 10w/v%.
Electrostatic spinning process parameter setting is as follows：Propeller promotes rate 1ml/h, electrostatic high-pressure 15kV.
Embodiment 4
A kind of electrostatic spinning reception device for preparing axial orientation tubular tissue engineering material, structure is same as Example 1, different
Part is：It is 40mm that two, which receive the distance between pole plate 10,；The distance of spinning nozzle 20 to reception pole plate 10 is 160mm.Volume
Axis 13 does the cycle of back and forth movement as 20s, effective travel 200mm, and the movement linear velocity of spool 13 is 20mm/s；Spool 13
Angular velocity of rotation is 1rad/s.
Embodiment 5
A kind of electrostatic spinning reception device for preparing axial orientation tubular tissue engineering material, structure is same as Example 1, different
Part is：It is 60mm that two, which receive the distance between pole plate 10,；The distance of spinning nozzle 20 to reception pole plate 10 is 180mm.Volume
Axis 13 does the cycle of back and forth movement as 20s, effective travel 600mm, and the movement linear velocity of spool 13 is 60mm/s；Spool 13
Angular velocity of rotation is 10rad/s.
Claims (10)
1. a kind of electrostatic spinning reception device for preparing axial orientation tubular tissue engineering material, including electrostatic spinning spinning nozzle,
Reception device is equipped with below spinning nozzle, reception device includes reception pole plate that is a pair of vertically arranged and being mutually parallel, and feature exists
In：The reception device further includes spool, and the spool receives pole plates perpendicular to two and sets, takes and be placed on two reception pole plates
Upper edge；The reception device further includes the propulsive mechanism that spool is promoted to do back and forth movement along the horizontal direction perpendicular to spool；Institute
It states and rotating mechanism is also associated on spool.
2. preparing the electrostatic spinning reception device of axial orientation tubular tissue engineering material as described in claim 1, feature exists
In：The distance of the spinning nozzle to reception pole plate is 140~200mm；Described two reception the distance between pole plates for 30~
60mm。
3. preparing the electrostatic spinning reception device of axial orientation tubular tissue engineering material as claimed in claim 2, feature exists
In：The spool does the cycle of back and forth movement as 5~20s, and effective travel is 200~600mm, and the movement linear velocity of spool is 20
~60mm/s, the angular velocity of rotation of the spool is 1~10rad/s.
4. the electrostatic spinning reception device of axial orientation tubular tissue engineering material is prepared as described in claim 1-3 is any,
It is characterized in that：The propulsive mechanism includes being horizontally placed on the screw received below pole plate perpendicular to spool；Spiral shell on the screw
Slide unit is connected to, the slide unit is equipped with pedestal, the spool support seat positioned at spool both ends is connected on the pedestal.
5. preparing the electrostatic spinning reception device of axial orientation tubular tissue engineering material as claimed in claim 4, feature exists
In：The rotating mechanism, which includes being arranged on, receives the pole plate both sides rack parallel with receiving pole plate, and the spool is equipped with and rack
Matched gear.
6. preparing the electrostatic spinning reception device of axial orientation tubular tissue engineering material as claimed in claim 4, feature exists
In：The positive and negative motor that the propulsive mechanism further includes control system and is connected with screw；The control system includes controller
The driver being connected with controller, the driver are equipped with power supply, and the driver is connected with positive and negative motor.
7. the electrostatic spinning that axial orientation tubular tissue engineering material is prepared as described in claim 1 or 2 or 3 or 5 or 6 receives dress
It puts, it is characterised in that：The spool is externally provided with water-soluble polymer coating.
8. preparing the electrostatic spinning reception device of axial orientation tubular tissue engineering material as claimed in claim 7, feature exists
In：The water-soluble polymer is selected from one or both of PEG, PEO, PVA combination of the above.
9. preparing the electrostatic spinning reception device of axial orientation tubular tissue engineering material as claimed in claim 8, feature exists
In：The spinning solution that the electrostatic spinning uses is fibroin albumen, collagen, l-lactic acid, polycaprolactone, l-lactic acid
The solution of one or more kinds of polymer in polycaprolactone co-polymer.
10. preparing the electrostatic spinning reception device of axial orientation tubular tissue engineering material as claimed in claim 7, feature exists
In：The spool by plater coat water-soluble polymer, the plater include upper mold and with upper mold it is matched under
Mould, the lower surface of upper mold and the upper surface of lower film enclose spool accommodating chamber, and spool accommodating chamber includes being arranged at pouring for stage casing
Chamber and the limit chamber for being arranged at both ends, the internal diameter of limit chamber, which is equal to spool outer diameter but is less than, pours intracavitary footpath；The plater
On be additionally provided with the pouring hole connected with pouring chamber.
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| CN108754634A (en) * | 2018-05-24 | 2018-11-06 | 广州迈普再生医学科技股份有限公司 | Electrostatic spinning reception device prepares the method and tubular bracket for being orientated electrostatic spinning tubular bracket |
| CN115323612A (en) * | 2022-08-19 | 2022-11-11 | 佛山清立新材料科技有限公司 | High-thermal-conductivity composite material and preparation method thereof |
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|---|---|---|---|---|
| CN108754634A (en) * | 2018-05-24 | 2018-11-06 | 广州迈普再生医学科技股份有限公司 | Electrostatic spinning reception device prepares the method and tubular bracket for being orientated electrostatic spinning tubular bracket |
| CN108754634B (en) * | 2018-05-24 | 2021-05-18 | 广州迈普再生医学科技股份有限公司 | Electrostatic spinning receiving device, method for preparing oriented electrostatic spinning tubular support and tubular support |
| CN115323612A (en) * | 2022-08-19 | 2022-11-11 | 佛山清立新材料科技有限公司 | High-thermal-conductivity composite material and preparation method thereof |
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