CN108102961B - Streptomyces samsunus and application thereof - Google Patents
Streptomyces samsunus and application thereof Download PDFInfo
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Abstract
The invention provides an actinomycete, which is streptomyces samsunus XJC-2-6, and the preservation number is CCTCC NO: m2017620. The streptomyces samsunus XJC-2-6 has antagonistic action on banana vascular wilt disease 4, banana physalospora piricola, banana gray leaf spot disease, banana tree canker, pepper anthracnose disease, colletotrichum gloeosporioides, strawberry anthracnose disease, botrytis cinerea, cucumber fusarium wilt disease, mango anthracnose disease, rice blast, wheat gibberellic disease, apple ring spot disease and the like, has wide development space in the prevention and treatment of banana vascular wilt disease, banana vascular leaf spot disease, banana vascular grey leaf spot disease, banana tree canker, pepper anthracnose disease, colletotrichum gloeosporioides, strawberry anthracnose, botrytis cinerea, cucumber fusarium wilt, mango anthracnose, rice blast, wheat scab, apple ring spot and the like, and has good development and application prospects.
Description
Technical Field
The invention belongs to the field of microorganisms, and particularly relates to streptomyces samsunus and application thereof.
Background
Banana vascular wilt, also known as panama disease and banana xanthophyll disease, is a vascular bundle wilting disease caused by fusarium oxysporum cubense f.sp. Pathogenic bacteria invade from the wound of the root of the host, spread to upper leaves through the vascular bundle via the corms and the pseudostems, block the xylem catheter, bring obstacles to the water transportation of plants and cause the plants to wither and die. Once a land is attacked by the destructive soil-borne disease, the pathogenic bacteria spread quickly and are difficult to cure radically, so the disease is called banana cancer. Therefore, the prevention and treatment of banana vascular wilt is imminent.
At present, no effective prevention and treatment method exists for the disease, and no ideal chemical agent and high-quality disease-resistant variety are available. Some cultivation management measures can only play a local control role. Moreover, long-term use of some chemical bactericides and modifying agents can easily cause the problem of drug resistance of pathogenic bacteria to be prominent, destroy the ecological environment of soil and be unsafe for human beings. The microbial pesticide can utilize microbes to generate disease-resistant substances, compete with pathogenic bacteria for nutrition and space sites, induce plants to generate disease resistance and the like, safely and effectively prevent and treat plant diseases, and has important application value in prevention and treatment of the plant diseases.
In conclusion, the environment-friendly, efficient and safe microbial bactericide has a wide development space in the prevention and treatment of banana vascular wilt, and is also a requirement for agricultural safe and sustainable development.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides an actinomycete, which is streptomyces samsonii, has good antagonism on banana vascular wilt, has wide development space in the prevention and treatment of banana vascular wilt, has good antagonism on various pathogenic bacteria, and has good development and application prospects.
The first aspect of the invention provides an actinomycete, which is streptomyces samsunus XJC-2-6, and the preservation number is CCTCC NO: m2017620.
A second aspect of the present invention provides the use of the actinomycetes according to the first aspect of the present invention for antagonizing Banana wilt bacterium No. 4, and/or Banana Leptospira, and/or Banana megaterium, and/or Banana canker, and/or Colletotrichum capsici, and/or Colletotrichum gloeosporioides, and/or Fragaria anachoriformis, and/or Botrytis cinerea, and/or Cucumis sativus, and/or mango Colletotrichum, and/or Pyricularia oryzae, and/or Gibberella cerealis, and/or Verticillium malorum.
The third aspect of the invention provides the use of the actinomycetes described in the first aspect of the invention for preventing and treating plant diseases caused by banana vascular wilt 4, and/or banana vascular wilt, and/or banana gray leaf spot, and/or banana tree canker, and/or pepper anthracnose, and/or colletotrichum gloeosporioides, and/or strawberry anthracnose, and/or botrytis cinerea, and/or cucumber vascular wilt, and/or mango anthracnose, and/or rice blast, and/or wheat gibberellic disease, and/or apple ring rot.
In a fourth aspect of the present invention, there is provided a fermentation broth of the actinomycete of the first aspect of the invention, or a filtrate of the fermentation broth, or an ethanol extract of the fermentation broth.
The fifth aspect of the invention provides the use of the fermentation broth of actinomycetes, or the filtrate of the fermentation broth, or the ethanol extract of the fermentation broth of actinomycetes, as defined in the fourth aspect of the invention, in antagonizing banana vascular wilt 4, and/or banana vascular rot, and/or banana gray spot, and/or banana tree canker, and/or pepper anthracnose, and/or colletotrichum gloeosporioides, and/or strawberry anthracnose, and/or botrytis cinerea, and/or cucumber vascular wilt, and/or mango anthracnose, and/or rice blast, and/or wheat gibberellic disease, and/or apple ring rot.
The sixth aspect of the present invention provides the use of the fermentation broth of actinomycetes, or the filtrate of the fermentation broth, or the ethanol extract of the fermentation broth of actinomycetes, as defined in the fourth aspect of the present invention, for preventing and treating plant diseases caused by banana vascular wilt 4, and/or banana alternaria, and/or banana gray spot, and/or banana tree canker, and/or pepper anthracnose, and/or colletotrichum gloeosporioides, and/or cucumber fusarium oxysporum, and/or mango anthracnose, and/or rice blast, and/or wheat gibberellic disease, and/or apple ring rot.
The seventh aspect of the present invention provides a microbial inoculum comprising the actinomycetes according to the first aspect of the present invention.
The Streptomyces samsunensis XJC-2-6(Streptomyces samsunensis XJC-2-6) has antagonistic action on banana vascular wilt pathogen No. 4, banana physalospora piricola, banana gray spot pathogen, banana tree canker pathogen, pepper anthracnose pathogen, colletotrichum gloeosporioides, strawberry anthracnose pathogen, botrytis cinerea, cucumber fusarium wilt pathogen, mango anthracnose pathogen, rice blast pathogen, wheat gibberellic disease, apple ring rot pathogen and the like, especially has obvious inhibition action on pepper anthracnose pathogen, banana tree canker pathogen, banana macrobotrytis cinerea and strawberry anthracnose pathogen, has the inhibition rate of more than 80.00 percent, and has the effects on banana vascular wilt, banana vascular rot, banana vascular grey spot disease, banana tree canker, pepper disease, colletotrichum gloeosporium, strawberry anthracnose, botrytis cinerea, cucumber blight, mango anthracnose, rice blast, black rot, banana vascular disease, banana vascular rot, banana vascular disease, black rot, banana, Has wide development space in the prevention and treatment of wheat scab, apple ring spot and the like, and has good development and application prospects.
Drawings
FIG. 1 is a phylogenetic tree of strain XJC-2-6 and related strains constructed based on the 16S rDNA sequence.
Detailed Description
The invention will be better understood by reference to the following examples.
The invention provides an actinomycete, which is streptomyces samsunensis XJC-2-6(streptomyces samsunensis XJC-2-6), and the preservation number is CCTCC NO: m2017620, the preservation date is 2017, 10 and 23, the preservation unit is China center for type culture Collection, and the address is Wuhan university in Wuhan, China. The streptomyces samsunus XJC-2-6 is obtained by separating and screening from a sample collected from south sea coral.
1 materials of the experiment
1.1 test soil
(1) Actinomycete separation soil sample
Collecting sample of south sea coral, mixing in sterile sealing bag, sealing, and storing in ice box. Collecting, and storing in refrigerator at 4 deg.C.
1.2 test Medium
The test culture medium of the experiment comprises a separation culture medium, a culture medium for observing culture characteristics, a physiological and biochemical culture medium, a fermentation culture medium and the like.
TABLE 1 isolation Medium for Actinomycetes and its formulation
TABLE 2 culture characteristic observation culture medium and its formulation
TABLE 3 culture media for physiological and biochemical characteristics
TABLE 4 fermentation media and their formulations
1.3 reagents and instrumentation used in this experiment
(1) Primary reagent
TABLE 5 major Biochemical reagents and sources
| Name of reagent | Manufacturer of the product |
| Bacterial genome DNA rapid extraction kit | BIOTEKE Corp. |
| 2xTaqPCR MasterMix | Bomade Biopsis Ltd |
| DNA marker | BEIJING COWIN BIOSCIENCE Co.,Ltd. |
| Agarose (agarose) | Promega Co.Ltd. |
(2) Apparatus and device
TABLE 6 Instrument and apparatus
1.4 test pathogens
The No. 4 physiological race of banana Fusarium oxysporum (FOC 4) is used for screening the anti-Fusarium oxysporum and measuring the bacteriostatic activity. Sciadophyllum bananas (Curvularia fallax), Sciadophora maxima (Curvularia lunata), Sciadophyllum bananas canker (Btoyophora dothidea), Colletotrichum capsici (Colletotrichum acutum), Colletotrichum gloeosporioides (Colletotrichum gloeosporioides), Fragaria ananasi (Colletotrichum fragaria), Botrytis cinerea (Botrytis cinerea), Cucumis sativus (Fusarium oxysporum (Schl.) F.cummerinum Owen), Coretotrichum Manshurica (Colletotrichum acratum), Pyricularia oryzae (Pyricularia oryzae Cav), Gibberella tritici (Fusarium graminearum), and Triticus (Fusarium graminearum) for the determination of antimicrobial spectra. The pathogenic bacteria tested above are all the strains preserved in the research room.
2 method of experiment
2.1 isolation and identification of rhizosphere soil Actinomycetes
2.1.1 isolation and purification of rhizosphere soil Actinomycetes
Naturally air drying the soil sample, fully grinding and sieving, weighing 1g of the soil sample, dissolving in 10mL of sterile water, heating at 55 ℃ for 20min, and placing in a shaking table at 180r/min for culturing for 20min to prepare suspension. Taking supernatant, diluting by 10 times dilution method to obtain 10-1、10-2And 10-3The soil suspension is respectively sucked with 0.1mL of suspension and coated on a separation culture medium, inverted culture is carried out for 2-4 weeks at 28 ℃, 3 times of gradient are arranged, different single colonies are selected and repeatedly purified on a YE culture medium by a scribing method.
2.1.2 screening of antagonistic bacteria
Adopting a plate opposing culture method: using a puncher with the diameter of 5mm to take the fungus cakes inoculated with 5d and consistent growth force of FOC4 pathogenic bacteria edges, inoculating the fungus cakes to the center of each PDA plate, inoculating the bacteria to be tested at a position 2.5cm away from the center of the colony of the pathogenic bacteria, inoculating 4 test strains to each dish, taking the pathogenic bacteria inoculated with FOC4 only as a control, placing the inoculated pathogenic bacteria in an incubator at 28 ℃ for inverted culture for 7d, and observing the result.
2.1.3 determination of in-dish antibiogram
Performing broad-spectrum determination on the strains by adopting a confrontation culture method: a5 mm puncher is used for taking a bacterial cake of 12 purified plant pathogenic bacteria, the bacterial cake is inoculated to the center of a PDA (personal digital assistant) flat plate, a small amount of bacteria to be detected are respectively inoculated to four points 2.5cm away from the bacterial cake of the pathogenic bacteria, a culture dish only inoculated with the pathogenic bacteria is used as a blank control group, and each treatment is repeated for 3 times. After culturing for 4-7 days in an incubator, measuring the colony growth diameter of pathogenic bacteria to be tested by adopting a cross measurement method, and counting the bacteriostasis rate according to the following formula:
colony diameter (mm) — average value of colony diameters measured-5.0
2.1.4 culture characteristic Observation of antagonistic Strain
Observations of the culture characteristics were made by reference to the standard medium used in the International Streptomyces program for the culture characteristics of Actinomycetes. Inoculating antagonistic actinomycetes on ISP2, ISP3, ISP4, ISP5, ISP6 and ISP7 culture media, culturing at 28 ℃ for 7-21d, and observing and recording culture characteristics of the strains on each culture media, including characteristics of colony morphology, aerial hypha production, spore color and intrastromal hypha color.
2.1.5 scanning Electron microscopy
Soaking the cover glass with potassium dichromate with the concentration of 0.05g/L, soaking and eluting with alcohol, washing with ultrapure water, blow-drying, and sterilizing at 121 ℃ for 20 minutes. The sterilized coverslip was inserted at 45 ℃ onto the culture medium of Goodpasture No. I inoculated with actinomycete strain, and cultured at 28 ℃ for 7-10 days. And (4) conveying the cover glass sample to a detection center for observation of a scanning electron microscope, placing the cover glass sample with the bacteria in a vacuum coating machine for spraying gold coating, and observing the fine structures on the surfaces of hyphae and spores of the strains by using the scanning electron microscope.
2.2.1.6 measurement of physiological and biochemical Properties
The physiological and biochemical identification of the strain is carried out by referring to the methods of Shirking, Xulihua and the like, and mainly comprises the following aspects:
(1) determination of enzymatic Properties
Firstly, urease experiments:
inoculating the strain on a urease culture medium, culturing for 4d at 28 ℃, and observing whether the culture medium is discolored. The test strains were tested for their ability to produce urease, and the medium was positive for pink and negative for no white.
② esterase (Tween 20, Tween 80) experiment:
streaked onto esterase medium for 1-2 weeks with daily observation. Positive if there is a faint halo around its growth, and negative if there is no halo.
③ hydrolyzing starch:
nutrient agar is used as a basic culture medium, and 1.0% of soluble starch is added. Inoculating the test strain on a flat plate, adopting a point inoculation method (the inoculation diameter is not more than 5mm), and when the strain grows well, dripping iodine solution around the bacterial colony for detection. If a transparent circle is generated around the strain, the generation of amylase is indicated, and the size of the circle indicates the strength of the amylase activity; if no amylase is produced, the color is blue.
Liquefaction of gelatin:
the strain was inoculated on the surface of gelatin medium without puncturing the medium, cultured at 28 ℃ and observed for the degree of liquefaction of the medium at 5d, 10d, 20d and 30d, respectively. Before observation, the test tube needs to be cooled for 20-30min or washed by tap water for 30min, and the liquefaction degree of the culture medium can be observed.
Cellulose decomposition:
immersing one end of the filter paper strip in a liquid culture medium, inoculating the strain to be detected on the filter paper sheet above the liquid level after sterilization, and observing whether the filter paper strip is decomposed or not after one month.
Sixthly, nitrate reduction:
the bacterial strain to be tested is inoculated in a nitrate reduction culture medium and cultured for 7 and 14 days at the temperature of 28 ℃, and the culture medium without inoculation is used as a control. A small amount of culture medium cultured for 7d and 14d was added to the test tube, and one drop of solution A and solution B was added, as was the case with the control. When the solution becomes pink, rose red, orange or brown, etc., the nitrate is positive in reduction; if no red color appears, 1 or 2 drops of diphenylamine reagent is dripped, and if the reagent is blue, the reduction effect is negative; if not blue, it is still treated as positive.
(2) Experiment for Using Single carbon Source
In the identification of actinomycetes, one of the important research indexes is the utilization of carbon sources, and different actinomycetes have different carbon source utilization capacities for sugars, alcohols, organic acids, fatty acids, and the like. Carbon source selected for the test: d-fructose, xylose, rhamnose, arabinose, raffinose, melezitose, anhydrous lactose, D-galactose, alpha-lactose, D-trehalose, D-mannose, D-ribose, inositol, sorbitol, mannitol, salicin and soluble starch are added into a basic culture medium of Pugodi according to the concentration of a carbon source of 1%. And inoculating the strain to be detected, culturing at constant temperature of 28 ℃ for 7-14d, taking the strain inoculated by a basic culture medium without any carbon source as a blank control, and observing the growth condition of the strain. If the strain can grow, the strain can utilize the carbon source; if the strain can not grow, the strain can not utilize the carbon source.
(3) Experiment for utilization of Single Nitrogen Source
Nitrogen sources selected for the test: histidine, methionine, serine, oxamic acid, glycine, hydroxyproline, phenylalanine, glutamic acid, cysteine, arginine, valine, ammonium molybdate tetrahydrate, ammonium acetate, ammonium nitrate, ammonium sulfate were added to the basal medium at a concentration of 0.5%. Inoculating strains, culturing at constant temperature of 28 deg.C for 7-14d, taking strains inoculated by basal medium without any nitrogen source as blank control, and observing growth condition of the strains. If the strain can grow, the strain can utilize the nitrogen source; if the strain can not grow, the strain can not utilize the nitrogen source.
(4) Measurement of other physiological and biochemical parameters
Temperature tolerance experiment:
inoculating the strain to be tested in the same culture medium, culturing at 20 deg.C, 24 deg.C, 28 deg.C, 32 deg.C and 36 deg.C for 7-14d under the condition of identical culture conditions, observing and recording the growth condition of bacterial colony, thereby determining the optimum temperature for strain growth.
② pH tolerance experiment:
the strains to be tested were inoculated into liquid media with pH values of 4, 5, 6, 7, 8, 9, and 10, respectively, and the other culture conditions were kept consistent, and the culture was carried out at 28 ℃ with observation every other week for four weeks. The growth of the strain was observed and recorded each time to determine the optimum pH for growth of the strain.
Salt tolerance test:
respectively inoculating the strains to be detected on NaCl (1%, 3%, 5%, 7%, 9%, 11%, 13%, 15%) culture media with different concentrations, wherein other nutrient components of the culture media are the same, culturing at 28 ℃, taking 7 days as an observation period, observing for 4 weeks, and recording whether the strains can grow so as to determine the upper and lower limit concentrations of NaCl which can be tolerated by the strains.
Hydrogen sulfide production experiment:
inoculating a strain to be detected on a hydrogen sulfide culture medium containing ammonium ions in a certain proportion, and culturing for 2 weeks at 28 ℃, wherein if the culture medium is black, hydrogen sulfide is generated; and if no black color exists, no hydrogen sulfide is generated.
2.1.7 molecular biological identification of antagonistic strains
(1) Extraction of actinomycete genomic DNA
Total DNA was extracted using biotek's kit for rapid extraction of bacterial genomic DNA (DP1301, Beijing Baitach Biotech Co., Ltd., China).
(2) Sequencing and analysis of 16S rDNA
PCR amplification of 16S rDNA:
PCR amplification was performed using actinomycete genomic DNA as a template and universal primers 27F and 1492R. The primer sequences are as follows: the upstream primer 27F (5 'AGAG TTTG ATCC TGGC TCAG 3'), and the downstream primer 1492R (5 'TACG GCTACCTT GTTA CGAC TT 3'). The specific reaction system is shown in Table 7, and the reaction procedure is shown in Table 8.
PCR reaction system of table 716S rDNA gene
TABLE 816 PCR amplification reaction conditions for S rDNA genes
Secondly, electrophoresis detection of PCR products:
after the PCR reaction is finished, 5 mu L of PCR amplification product is taken to carry out electrophoresis detection on the PCR product of the strain on 1% agarose gel, and whether the connection is successful is determined according to the length of the target fragment.
Sequencing and constructing phylogenetic tree:
the PCR product of the strain was sent to Huada Gene Co for sequencing. The determined gene sequences were aligned using BLAST software and compared for homology to the known 16S rDNA in GenBank and ezbiocoud databases. Finding out sequences with higher homology to perform multiple matching arrangement analysis, and adopting MEGA5.1 software to perform clustering analysis and phylogenetic tree construction by using a Neighbor-Joining method.
2.3 evaluation of bacteriostatic Activity of antagonistic Strain
2.3.1 determination of plate-confronted broad-spectrum antibacterial activity
Performing broad-spectrum determination on the strains by adopting a plate confrontation method: and (3) taking a purified bacterial cake of 12 plant pathogenic bacteria by using a 5mm puncher, inoculating the bacterial cake to the center of a PDA (personal digital assistant) flat plate, respectively inoculating a small amount of bacteria to be detected at four points 2.5cm away from the bacterial cake of the pathogenic bacteria, taking a culture dish only inoculated with the pathogenic bacteria as a blank control group, and repeating the treatment for 3 times. After culturing for 4-7 days in an incubator, measuring the colony growth diameter of pathogenic bacteria to be tested by adopting a cross method, and counting the bacteriostasis rate according to the following formula:
colony diameter (mm) — average value of colony diameters measured-5.0
2.3.2 measurement of bacteriostatic Activity of Secondary metabolites against pathogenic bacteria
Respectively inoculating the strains into an SLM liquid culture medium for culturing for 7 days, adding absolute ethyl alcohol, oscillating for 10 days, carrying out vacuum filtration on 5 layers of filter paper, and collecting secondary metabolites of the strains as samples. Taking the sterilized culture medium, pouring the culture medium onto a flat plate by using an antibacterial paper sheet method, preparing a filter paper sheet with the diameter of 9mm by using a puncher after the flat plate is solidified, carrying out dry heat sterilization on the filter paper sheet for 2h, soaking the filter paper sheet in a sample in an aseptic operating platform for 30min, taking out and volatilizing a solvent, flatly pasting the filter paper sheet on the medicine-containing flat plate, and simultaneously inoculating an indicator bacterium in the center of the culture medium. And finally, placing all culture dishes in a constant-temperature incubator at 28 ℃ for culture, observing every day, recording and photographing, and calculating the average value of the diameters of the inhibition zones by making three parallels for each group.
2 results and analysis
2.1 screening and morphological characterization of Actinomycetes
Carrying out plate coating separation and streaking on the purified strains, removing the weight according to the colony morphology and the color of the strains on a purification culture medium, and carrying out primary screening by a plate opposition culture method and secondary screening by an Oxford cup method to obtain 1 actinomycete generating the largest inhibition zone; the number is XJC-2-6.
TABLE 9 culture characteristics of Strain XJC-2-6 on 7 media
2.2 physiological and Biochemical characteristics of the Strain
TABLE 10 part of the physio-biochemical characteristics of Strain XJC-2-6
+: the result is positive; -: the result was negative.
2.3 phylogenetic characteristics of the strains
Extracting total DNA of the strain XJC-2-6, obtaining a 16S rDNA sequence of about 1.5kb through PCR amplification, sequencing to obtain a sequence thereof, submitting sequence information to EzTaxon for gene sequence similarity search to obtain sequence information of 10 named model bacteria with highest homology with the strain XJC-2-6, performing phylogenetic analysis, and constructing a phylogenetic tree (figure 1). As can be seen from the figure, XJC-2-6 is integrated with Streptomyces to form a branch, and the homology of the 16S rDNA sequence is in the range of 97.47-98.94%; among them, the homology with Streptomyces samsunensis was the highest and 98.94%.
By combining morphological characteristics, physiological and biochemical characteristics and 16s DNA molecular sequence analysis results, the strain XJC-2-6 is identified to be Streptomyces samsunensis (Streptomyces samsunensis), and is named as Streptomyces samsunensis XJC-2-6(Streptomyces samsunensis XJC-2-6).
2.4 evaluation of antibacterial Activity of Strain XJC-2-6
2.4.1 antagonistic action of the plates on pathogenic bacteria
TABLE 11 inhibitory Effect of Strain XJC-2-6 on 12 pathogenic fungi
The data in the table are mean ± standard deviation. The different small letters in the same column indicate significant differences at P < 0.05 as tested by Duncan's New Complex Pole Difference method.
As can be seen from Table 11, the strain XJC-2-6 of the present invention has good inhibitory effects on 12 pathogenic fungi.
The embodiments of the present invention have been described in detail, but the embodiments are merely examples, and the present invention is not limited to the embodiments described above. Any equivalent modifications and substitutions to those skilled in the art are also within the scope of the present invention. Accordingly, equivalent changes and modifications made without departing from the spirit and scope of the present invention should be covered by the present invention.
Claims (7)
1. An actinomycete, which is streptomyces samsunus XJC-2-6, and has a preservation number of CCTCC NO: m2017620.
2. Use of an actinomycete according to claim 1 for antagonising Fusarium oxysporum f.sp.cubense rate 4, and/or Fusarium oxysporum f.sp.cubense f.villax, and/or Curvularia lunata lucanta, and/or btorosphaeria dothidea, and/or Colletotrichum capsici, Colletotrichum acratum, and/or Colletotrichum gloeosporoides, and/or Colletotrichum strawberrii, and/or Colletotrichum Botrytis cinerea, and/or Fusarium oxysporum (Schl.) f.cubense Ojnin, merrinum mango, and/or trichothecium verticillium, and/or Fusarium oxysporum, Fusarium oxysporum graminearum, and rice blight/or rice blight/rice blight and rice blight/rice blight.
3. Use of an actinomycete according to claim 1 for controlling a disease in a plant of Fusarium oxysporum f.sp.Cubense Race 4, and/or Fusarium oxysporum Fallax, and/or Fusarium macrosporum Curvularia lunata, and/or Banana tree canker pathogen Btoyophora dothiea, and/or Colletotrichum capsici, Colletotrichum acratum, and/or Colletotrichum gloeosporioides, and/or Colletotrichum fragrans, and/or Colletotrichum cinerea, and/or Colletotrichum Botrytis, and/or cucumber Fusarium oxysporum (Schl.) F.cu. chrysin, mango and/or Colletotrichum verticillium, and/or Fusarium oxysporum, Fusarium oxysporum solanum, Fusarium oxysporum, and/or Fusarium graminearum, Fusarium graminearum and/or Fusarium graminearum oryzae.
4. A fermentation broth of the actinomycete of claim 1, or a filtrate of the fermentation broth, or an ethanol extract of the fermentation broth.
5. Use of a strain broth or an ethanol extract of a filtrate or a broth of a strain broth or a broth according to claim 4 for antagonising Fusarium oxysporum f.sp.cubense rate 4, and/or Curvularia lunata fallax, and/or Curvularia masana lunata, and/or Fusarium oxysporum lunata, and/or Fusarium graminearum dothioides, and/or Fusarium capsici, and/or Colletotrichum acutum, and/or Colletotrichum gloosporioides, and/or Fusarium oxysporum (Schl.) f.sp.sp.cucumerium, and/or verticillium orbicularum, and/or Fusarium oxysporum f.sp.f.sp.cereum, and/or Fusarium oxysporum frugium, and/or Fusarium graminearum, Fusarium oxysporum, and/or Fusarium graminearum, Fusarium graminearum and/or Fusarium graminearum oryzae.
6. Use of a strain broth or an ethanol extract of a filtrate or a broth of a fermentation broth or of a filtrate of a strain broth or of a broth according to claim 4 for the control of Fusarium oxysporum f.sp.Cubense Race 4, and/or Curvularia canadensis, and/or Curvularia lunata, and/or Fusarium oxysporum, and/or Fusarium solani, and/or Fusarium capsici, Coletonrichum acutum, and/or Colletotrichum gloosporioides, and/or Colletotrichum fragrans, and/or Botrytis botrys cinerea, and/or Fusarium oxysporum (Schl.) F.sp.faecal, and/or Fusarium verticillarum, Fusarium oxysporum, and/or Fusarium oxysporum oryzae, Fusarium oxysporum, and/or Fusarium oxysporum graminearum, Fusarium oxysporum and/or Fusarium graminum.
7. A microbial preparation comprising the actinomycetes according to claim 1.
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| CN109207408B (en) * | 2018-10-26 | 2021-09-24 | 云南省热带作物科学研究所 | Biocontrol antagonistic actinomycete for rubber tree root disease and application thereof |
| CN111096337B (en) * | 2020-01-16 | 2020-11-10 | 中国科学院南海海洋研究所 | Application of deep sea actinomycete biological preparation in preparing medicine for preventing citrus canker disease |
| CN113846126B (en) * | 2021-10-15 | 2023-08-04 | 中国热带农业科学院热带生物技术研究所 | Preparation method and application of banana vascular wilt resistant small molecular compound |
| CN114540213B (en) * | 2021-11-11 | 2024-03-19 | 中国热带农业科学院海口实验站 | Actinomycetes with antibacterial activity and application thereof |
| CN114214224B (en) * | 2021-11-24 | 2023-06-13 | 中国热带农业科学院海口实验站 | Biocontrol bacterium for preventing and controlling anthrax pathogenic bacteria and application thereof |
| CN115125157B (en) * | 2021-12-30 | 2023-04-18 | 中国热带农业科学院南亚热带作物研究所 | Streptomyces for antagonizing pathogenic microorganisms of crops and application thereof |
| CN116875646B (en) * | 2023-09-06 | 2023-12-19 | 中国热带农业科学院三亚研究院 | Preparation and application of banana vascular wilt resistant macrolide compound |
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