CN108102919A - A kind of method and its application for improving chicken eimeria tenella egg capsule Sporulated efficiency - Google Patents
A kind of method and its application for improving chicken eimeria tenella egg capsule Sporulated efficiency Download PDFInfo
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- CN108102919A CN108102919A CN201711495787.3A CN201711495787A CN108102919A CN 108102919 A CN108102919 A CN 108102919A CN 201711495787 A CN201711495787 A CN 201711495787A CN 108102919 A CN108102919 A CN 108102919A
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- sporulated
- egg capsule
- efficiency
- oxygen
- liquid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/10—Protozoa; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N3/00—Spore forming or isolating processes
Abstract
The present invention relates to a kind of new methods for improving chicken eimeria tenella egg capsule Sporulated efficiency, more particularly to a kind of by the method for the chicken eimeria tenella egg capsule Sporulated efficiency that egg capsule concentration, liquid level and oxygen concentration is controlled to improve, this method can greatly improve the efficiency of chicken eimeria tenella egg capsule Sporulated, manpower and materials input is reduced, improves production efficiency.
Description
Technical field
The invention belongs to veterinary biologics field, especially a kind of raising chicken eimeria tenella egg capsule Sporulated efficiency
Method.
Background technology
In the production process of vaccine to chicken coccidiosis, the Sporulated culture of egg capsule is a very crucial link.Original ovum
Requirement of the cystospore culture technique to egg capsule concentration constrains the production efficiency of its scale significantly.To improve Ji Aimeier
The Sporulated efficiency of coccidian oocyst, scientific research personnel have carried out a large number of experiments Journal of Sex Research.
The content of the invention
It is an object of the invention to provide a kind of new method for improving chicken eimeria tenella egg capsule Sporulated efficiency, this method
The Sporulated efficiency of chicken eimeria tenella egg capsule can be greatly improved, improves production efficiency.
The present invention is realized by following technical method：
A kind of method of raising chicken eimeria tenella egg capsule Sporulated efficiency is as follows：
(1) concentration mensuration is carried out to the unsporulated oocysts liquid harvested.
(2) concentration of unsporulated oocysts liquid is adjusted to ten thousand egg capsule/ml of 50-1000 with reagent.
(3) the unsporulated oocysts liquid that concentration is ten thousand egg capsule/ml of 50-1000 is poured into 2000 milliliters of beakers, liquid level
Highly control is in 10-35cm.
(4) logical oxygen tube at one end is inserted into unsporulated oocysts liquid bottom and fixed, the other end goes out with oxygen flow meter
Gas end is connected, and the inlet end of oxygen flow meter is connected with oxygen cylinder.
(5) beaker for connecting logical oxygen conduit is put into constant temperature oscillation case and fixation, design temperature is 20-30 DEG C, rotating speed
For 100r/min, start Sporulated culture.
(6) oxygen cylinder switch is opened after 1h, oxygen-supply quantity is set as 1-5m3/ 1000ml/h, culture is for 24 hours.
(7) for 24 hours after oxygen-supply quantity is set as 0.2-1m3/ 1000ml/h continues to cultivate.
(8) start to detect Sporulated rate after cultivating 48h, every 0.5h detections once, until Sporulated rate qualification can terminate
The good egg capsule liquid of Sporulated is packed into special container sealing by culture, i.e. Sporulated is completed.
Specifically, described in step 2) adjustment unsporulated oocysts liquid concentration reagent can be 2.5% potassium bichromate,
It is any in 0.05%-0.2% toluene-sodium-sulfonchloramides or 0.01%-0.5% chloramine B threes.
Specifically, the standard of the Sporulated rate qualification described in step 8) is：Sporulated rate is not less than 80%.
Advantages of the present invention and advantageous effect are：
The present invention overcomes traditional chicken eimeria tenella egg capsule coccidia Sporulated efficiency is low, manpower and materials requirement is high
Defect passes through chicken eimeria tenella egg capsule Sporulated efficiency that egg capsule concentration, liquid level and oxygen concentration is controlled to improve
Method substantially increases the Sporulated efficiency of chicken eimeria tenella egg capsule, reduces manpower and materials input, improves production efficiency.
Specific embodiment
Contrast experiment：
6,000,000,000 non-Sporulated Eimeria Tenella egg capsules just harvested are taken, are bisected into three groups, 2,000,000,000/group.
Group one：Routinely Sporulated technology carries out Sporulated culture, with 0.2 toluene-sodium-sulfonchloramide adjustment egg capsule concentration for 400,000/
Ml, is poured into 2000ml beakers totally by 5000 milliliters, and liquid level 2cm is put into isothermal vibration case, it is necessary to 25 2000ml beakers
Middle culture, 28 DEG C of temperature, rotating speed 100r/min.Start to detect Sporulated rate after 48h, until Sporulated rate >=80% when terminate.
Group two carries out Sporulated culture by the method for the present invention, is 5,000,000/ml with 0.2 toluene-sodium-sulfonchloramide adjustment egg capsule concentration, altogether
It 400 milliliters, pours into 2000ml beakers, liquid level is not higher than 35cm, it is necessary to which 1 2000ml beaker, installs logical oxygen additional and lead respectively
Pipe, is put into isothermal vibration case and cultivates, 28 DEG C of temperature, rotating speed 100r/min.After cultivating 1h, lead to oxygen, oxygen-supply quantity is set as
2m3/ 1000ml/h, oxygen-supply quantity is set as 1m afterwards for 24 hours3Start to detect Sporulated rate after/1000ml/h, 48h, until Sporulated rate
Terminate when >=80%.
Group three is 5,000,000/ml with 0.2 toluene-sodium-sulfonchloramide adjustment egg capsule concentration, totally 400 milliliters, is poured into 2000ml beakers, liquid
Face height is not higher than 35cm, it is necessary to 1 2000ml beaker, is put into isothermal vibration case and cultivates, 28 DEG C of temperature, rotating speed 100r/
min.Start to detect Sporulated rate after 48h.
Result of the test：
Group one when Sporulated rate is 79%, 50h when in 48h, Sporulated rate is 70%, 49h Sporulated rate be 88%.
Group two when Sporulated rate is 89%, 50h when in 48h, Sporulated rate is 83%, 49h Sporulated rate be 91%.
Group three when Sporulated rate is 49%, 50h when in 48h, Sporulated rate is 47%, 49h Sporulated rate be 50%.
Interpretation of result：
Same unsporulated oocysts, during using Sporulated culture technique of the present invention, egg capsule concentration is high, few using container,
It is few using oscillator, Sporulated faster, Sporulated rate higher.The Sporulated efficiency of your egg capsule of chicken Amy is greatly improved, is improved
Production efficiency.
Claims (3)
1. a kind of method for improving chicken eimeria tenella egg capsule Sporulated efficiency, comprises the following steps：
(1) concentration mensuration is carried out to the unsporulated oocysts liquid harvested.
(2) concentration of unsporulated oocysts liquid is adjusted to ten thousand egg capsule/ml of 50-1000 with reagent.
(3) the unsporulated oocysts liquid that concentration is ten thousand egg capsule/ml of 50-1000 is poured into 2000 milliliters of beakers, liquid level
Control is in 10-35cm.
(4) logical oxygen tube at one end is inserted into unsporulated oocysts liquid bottom and fixed, the other end and oxygen flow meter outlet side
It is connected, the inlet end of oxygen flow meter is connected with oxygen cylinder.
(5) beaker for connecting logical oxygen conduit is put into constant temperature oscillation case and fixation, design temperature is 20-30 DEG C, and rotating speed is
100r/min starts Sporulated culture.
(6) oxygen cylinder switch is opened after 1h, oxygen-supply quantity is set as 1-5m3/ 1000ml/h, culture is for 24 hours.
(7) for 24 hours after oxygen-supply quantity is set as 0.2-1m3/ 1000ml/h continues to cultivate.
(8) start to detect Sporulated rate after cultivating 48h, every 0.5h detections once, until Sporulated rate qualification can terminate to cultivate,
The good egg capsule liquid of Sporulated is packed into special container sealing, i.e. Sporulated is completed.
A kind of 2. method for improving chicken eimeria tenella egg capsule Sporulated efficiency as described in claim 1, which is characterized in that step
It is rapid 2) described in adjustment unsporulated oocysts liquid concentration reagent can be 2.5% potassium bichromate, 0.05%-0.2% toluene-sodium-sulfonchloramides
It is or any in 0.01%-0.5% chloramine B threes.
A kind of 3. method for improving chicken eimeria tenella egg capsule Sporulated efficiency as described in claim 1, which is characterized in that step
It is rapid 8) described in the standard of Sporulated rate qualification be：Sporulated rate is not less than 80%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711495787.3A CN108102919A (en) | 2017-12-31 | 2017-12-31 | A kind of method and its application for improving chicken eimeria tenella egg capsule Sporulated efficiency |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711495787.3A CN108102919A (en) | 2017-12-31 | 2017-12-31 | A kind of method and its application for improving chicken eimeria tenella egg capsule Sporulated efficiency |
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| Publication Number | Publication Date |
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| CN108102919A true CN108102919A (en) | 2018-06-01 |
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| CN201711495787.3A Withdrawn CN108102919A (en) | 2017-12-31 | 2017-12-31 | A kind of method and its application for improving chicken eimeria tenella egg capsule Sporulated efficiency |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005097971A1 (en) * | 2004-03-31 | 2005-10-20 | Embrex, Inc. | Method of purifying oocysts using enzymatic digestion of fecal debris |
| CN101045159A (en) * | 2007-04-30 | 2007-10-03 | 吉林农业大学 | Method for preparing vaccine to chicken coccidiosis |
| CN101659935A (en) * | 2009-09-01 | 2010-03-03 | 佛山市正典生物技术有限公司 | Application of adjustable hyperstatic oxygen-increasing machine in coccidian oocyst sporulation development |
| CN103251938A (en) * | 2013-04-15 | 2013-08-21 | 内蒙古神元生物工程股份有限公司 | Industrial production process of eimeria coccidiosis attenuated vaccine |
| CN103374526A (en) * | 2012-04-18 | 2013-10-30 | 上海市农业科学院 | Coccidian oocyst culture preservative fluid and application method thereof |
| CN103710265A (en) * | 2013-12-23 | 2014-04-09 | 广东省农业科学院动物卫生研究所 | Application of chloramine B solution in chicken coccidiosis oocyst culture and preservation |
| CN106459893A (en) * | 2014-04-03 | 2017-02-22 | 英特维特国际股份有限公司 | Method to sporulate coccidial oocysts purified from animal faeces, sporulated oocysts obtained with this method and a vaccine containing these sporulated oocysts |
-
2017
- 2017-12-31 CN CN201711495787.3A patent/CN108102919A/en not_active Withdrawn
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005097971A1 (en) * | 2004-03-31 | 2005-10-20 | Embrex, Inc. | Method of purifying oocysts using enzymatic digestion of fecal debris |
| CN101045159A (en) * | 2007-04-30 | 2007-10-03 | 吉林农业大学 | Method for preparing vaccine to chicken coccidiosis |
| CN101659935A (en) * | 2009-09-01 | 2010-03-03 | 佛山市正典生物技术有限公司 | Application of adjustable hyperstatic oxygen-increasing machine in coccidian oocyst sporulation development |
| CN103374526A (en) * | 2012-04-18 | 2013-10-30 | 上海市农业科学院 | Coccidian oocyst culture preservative fluid and application method thereof |
| CN103251938A (en) * | 2013-04-15 | 2013-08-21 | 内蒙古神元生物工程股份有限公司 | Industrial production process of eimeria coccidiosis attenuated vaccine |
| CN103710265A (en) * | 2013-12-23 | 2014-04-09 | 广东省农业科学院动物卫生研究所 | Application of chloramine B solution in chicken coccidiosis oocyst culture and preservation |
| CN106459893A (en) * | 2014-04-03 | 2017-02-22 | 英特维特国际股份有限公司 | Method to sporulate coccidial oocysts purified from animal faeces, sporulated oocysts obtained with this method and a vaccine containing these sporulated oocysts |
Non-Patent Citations (1)
| Title |
|---|
| AHMADREZA FATEMI 等: "Effects of Artemisia annua extracts on sporulation of Eimeria oocysts", 《PARASITOL RES》 * |
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