CN108102882A - Microbial cell wall-breaking machine - Google Patents
Microbial cell wall-breaking machine Download PDFInfo
- Publication number
- CN108102882A CN108102882A CN201810114722.8A CN201810114722A CN108102882A CN 108102882 A CN108102882 A CN 108102882A CN 201810114722 A CN201810114722 A CN 201810114722A CN 108102882 A CN108102882 A CN 108102882A
- Authority
- CN
- China
- Prior art keywords
- rotor
- microbial cell
- wall
- breaking machine
- stator
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000000813 microbial effect Effects 0.000 title claims abstract description 56
- 239000002245 particle Substances 0.000 claims abstract description 48
- 239000012530 fluid Substances 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 5
- 229910001220 stainless steel Inorganic materials 0.000 claims description 3
- 230000005611 electricity Effects 0.000 claims 1
- 230000009514 concussion Effects 0.000 abstract description 4
- 238000004880 explosion Methods 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Crushing And Pulverization Processes (AREA)
Abstract
The present invention provides a kind of microbial cell wall-breaking machine, and broken wall treatment is carried out to microbial cell fluid, including：Stator, rotor, motor；The stator surrounds broken wall space with rotor, the several first raised particles are provided on the stator wall surface opposite with the rotor, the several second raised particles are provided on the rotor wall surface opposite with the stator, several first raised particle is shifted to install with the described several second raised particle holdings, the rotor has shaft, the motor drives the rotor to be rotated with its shaft, and the rotor provides operating on centrifugal force to the microbial cell fluid in broken wall space.The microbial cell wall-breaking machine of the present invention is collided and squeezed to microbial cell fluid by raised particle, realizes the scattered of cell cenobium.Meanwhile by centrifugal action, the high frequency between cell flow and raised particle is made to rub to form the miniature concussion explosion of cell, obtains minimum 67 μm of microbial cell particle.
Description
Technical field
The present invention relates to a kind of wall-breaking machine more particularly to a kind of microorganisms that broken wall treatment is carried out to microbial cell fluid
Breaking-wall cell machine.
Background technology
At present, need to carry out broken wall treatment for microbial cell fluid, to meet the needs of experiment.It is however, existing
Food broken wall treatment of the wall-breaking machine mostly suitable for family often can not effectively carry out microbial cell fluid sufficient
Broken wall.Therefore, in view of the above-mentioned problems, it is necessary to propose further solution.
The content of the invention
It is an object of the invention to provide a kind of microbial cell wall-breaking machine, to overcome the deficiencies in the prior art.
For achieving the above object, a kind of microbial cell wall-breaking machine of offer of the invention, to microbial cell stream
Body carries out broken wall treatment, including：Stator, rotor, motor；
The stator surrounds broken wall space with rotor, and it is convex to be provided with several first on the stator wall surface opposite with the rotor
Particle is played, the several second raised particles, several first protrusions are provided on the rotor wall surface opposite with the stator
Particle is shifted to install with the described several second raised particle holdings, and the rotor has shaft, and the motor drives the rotor
It is rotated with its shaft, the rotor provides operating on centrifugal force to the microbial cell fluid in broken wall space.
The improvement of microbial cell wall-breaking machine as the present invention, the stator are the outer of the microbial cell wall-breaking machine
Shell, the rotor are the cylinder in the shell.
The improvement of microbial cell wall-breaking machine as the present invention, several first raised particle are arranged in the form of an array
In on the developed surface of the madial wall of the shell, several second raised particle is arranged in the outer of the cylinder in the form of an array
On the developed surface of side wall.
The improvement of microbial cell wall-breaking machine as the present invention, the microbial cell wall-breaking machine further include cover board and bottom
Seat, the stator and rotor are installed on the pedestal, and the cover board is fastened on the upper end of the stator and rotor.
The improvement of microbial cell wall-breaking machine as the present invention, the motor are fixed on the lid by motor mount
On plate, the shaft of the motor is pivotally connected through the cover board with the rotor.
The improvement of microbial cell wall-breaking machine as the present invention, the broken wall space have water inlet, the water inlet
It is set respectively close to the top and bottom in the broken wall space.
The improvement of microbial cell wall-breaking machine as the present invention, described first, second raised particle is hemisphere.
The improvement of microbial cell wall-breaking machine as the present invention, between the described first raised particle and the second raised particle
Spacing be 1cm.
It is the improvement of microbial cell wall-breaking machine as the present invention, the stator and the first raised particle thereon, described
Rotor and the second raised particle thereon are 316 stainless steels.
The improvement of microbial cell wall-breaking machine as the present invention, the rotating speed of the rotor is 3500-500r/min.
Compared with prior art, the beneficial effects of the invention are as follows：The microbial cell wall-breaking machine of the present invention passes through protrusion
Grain is collided and squeezed to microbial cell fluid, realizes the scattered of cell cenobium.Meanwhile by centrifugal action, make cell
High frequency between fluid and raised particle rubs to form the miniature concussion explosion of cell, obtains minimum 6-7 μm of microbial cell
Particle.
Description of the drawings
Fig. 1 is the internal structure schematic diagram of a specific embodiment of the microbial cell wall-breaking machine of the present invention.
Specific embodiment
The present invention is described in detail for shown each embodiment below in conjunction with the accompanying drawings, but it should explanation, these
Embodiment is not limitation of the present invention, those of ordinary skill in the art according to these embodiment institute work energy, method,
Or equivalent transformation or replacement in structure, all belong to the scope of protection of the present invention within.
As shown in Figure 1, the microbial cell wall-breaking machine of the present invention is used to carry out broken wall treatment to microbial cell fluid,
Including：Stator 1, rotor 2, motor 3.
The stator 1 surrounds the broken wall space 11 of microbial cell fluid with rotor 2, which has water inlet
111.In one embodiment, the stator 1 be the microbial cell wall-breaking machine columnar shell, the rotor 2
To be located at the cylinder in the shell.Preferably, the shell keeps being coaxially disposed with cylinder.In addition, the microbial cell
Wall-breaking machine further includes cover board 4 and pedestal 5, and the stator 1 and rotor 2 are installed on the pedestal 5, and the cover board 4 is fastened on institute
The upper end of stator 1 and rotor 2 is stated, so with the broken wall treatment space of formation closing.At this point, the water inlet 111 respectively close to
The top and bottom in the broken wall space 11 surrounded are set.In other embodiments, columnar shell structure can also be set
For rotor 2, internal cylinder is arranged to stator 1.
In order to which microbial cell fluid is collided and squeezed, set on the stator 1 wall surface opposite with the rotor 2
The several first raised particles 12 are equipped with, the several second raised particles are provided on the rotor 2 wall surface opposite with the stator 1
13.Wherein, the described several first raised particles 12 are shifted to install with the described several second raised holdings of particle 13.
Based on the cylinder and cylindrical housing in above-described embodiment, several first raised particle 12 is arranged in the form of an array
It is distributed on the developed surface of the madial wall of the shell, several second raised particle 13 is arranged in the cylinder in the form of an array
Lateral wall developed surface on.Preferably, the stator 1 and the first raised particle 12 thereon, the rotor 2 and thereon the
Two raised particles 13 are 316 stainless steels.It is further preferred that described first, second raised particle 12,13 is hemisphere, hemisphere
The raised particle of shape can be integrally formed with the stator 1 and rotor 2.First raised 12 and the second protrusion particle 13 of particle it
Between spacing be 1cm.
The rotor 2 has shaft, and the motor 3 drives the rotor 2 to be rotated with its shaft.So as to described turn
Son 2 can drive microbial cell wall-breaking machine in broken wall space 11 to carry out high-speed rotation, and then to the microorganism in broken wall space 11
Cell flow provides operating on centrifugal force.Under the operating on centrifugal force, make the high frequency friction shape between cell flow and raised particle
It explodes into the miniature concussion of cell, obtains minimum 6-7 μm of microbial cell particle.Further, in order to realize the peace of motor 3
Dress is fixed, and the microbial cell wall-breaking machine further includes motor mount 6, and the motor 3 is fixed by the motor mount 6
In on the cover board 4, at this point, the shaft of the motor 3 is pivotally connected through the cover board 4 with the rotor 2.Preferably, institute
The rotating speed for stating rotor 2 is 3500-500r/min.
In conclusion the microbial cell wall-breaking machine of the present invention collides microbial cell fluid by raised particle
And extruding, realize the scattered of cell cenobium.Meanwhile by centrifugal action, the high frequency between cell flow and raised particle is made to rub
Wiping forms the miniature concussion explosion of cell, obtains minimum 6-7 μm of microbial cell particle.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Profit requirement rather than above description limit, it is intended that all by what is fallen within the meaning and scope of the equivalent requirements of the claims
Variation is included within the present invention.Any reference numeral in claim should not be considered as to the involved claim of limitation.
Moreover, it will be appreciated that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
Using specification as an entirety, the technical solutions in each embodiment can also be properly combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (10)
1. a kind of microbial cell wall-breaking machine carries out broken wall treatment to microbial cell fluid, including：Stator, rotor, electricity
Machine；It is characterized in that：
The stator surrounds broken wall space with rotor, and it is convex to be provided with several first on the stator wall surface opposite with the rotor
Particle is played, the several second raised particles, several first protrusions are provided on the rotor wall surface opposite with the stator
Particle is shifted to install with the described several second raised particle holdings, and the rotor has shaft, and the motor drives the rotor
It is rotated with its shaft, the rotor provides operating on centrifugal force to the microbial cell fluid in broken wall space.
2. microbial cell wall-breaking machine according to claim 1, which is characterized in that the stator is the microbial cell
The shell of wall-breaking machine, the rotor are the cylinder in the shell.
3. microbial cell wall-breaking machine according to claim 2, which is characterized in that several first raised particle is with battle array
Row form is arranged on the developed surface of the madial wall of the shell, and several second raised particle is arranged in institute in the form of an array
On the developed surface for stating the lateral wall of cylinder.
4. microbial cell wall-breaking machine according to claim 2, which is characterized in that the microbial cell wall-breaking machine also wraps
Cover board and pedestal are included, the stator and rotor are installed on the pedestal, and the cover board is fastened on the upper of the stator and rotor
End.
5. microbial cell wall-breaking machine according to claim 2, which is characterized in that the motor is consolidated by motor mount
Due on the cover board, the shaft of the motor is pivotally connected through the cover board with the rotor.
6. microbial cell wall-breaking machine according to claim 1, which is characterized in that the broken wall space has water inlet,
The water inlet is set respectively close to the top and bottom in the broken wall space.
7. microbial cell wall-breaking machine according to claim 1, which is characterized in that the first, second raised particle is
Hemisphere.
8. microbial cell wall-breaking machine according to claim 1, which is characterized in that the first raised particle and second convex
It is 1cm to play the spacing between particle.
9. microbial cell wall-breaking machine according to claim 1, which is characterized in that the stator and thereon first protrusion
Particle, the rotor and the second raised particle thereon are 316 stainless steels.
10. microbial cell wall-breaking machine according to claim 1, which is characterized in that the rotating speed of the rotor is 3500-
500r/min。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810114722.8A CN108102882A (en) | 2018-02-06 | 2018-02-06 | Microbial cell wall-breaking machine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810114722.8A CN108102882A (en) | 2018-02-06 | 2018-02-06 | Microbial cell wall-breaking machine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108102882A true CN108102882A (en) | 2018-06-01 |
Family
ID=62221690
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810114722.8A Pending CN108102882A (en) | 2018-02-06 | 2018-02-06 | Microbial cell wall-breaking machine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108102882A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111606476A (en) * | 2020-05-26 | 2020-09-01 | 郑州众然供应链管理有限公司 | Microorganism inactivation device |
| CN113122439A (en) * | 2021-05-08 | 2021-07-16 | 王琼 | Device for extracting protein and nucleic acid by breaking wall of fungus and method for extracting protein and nucleic acid by applying device |
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|---|---|---|---|---|
| US4562972A (en) * | 1983-04-13 | 1986-01-07 | Kawasaki Jukogyo Kabushiki Kaisha | Micropulverizer |
| EP0303791A1 (en) * | 1987-08-21 | 1989-02-22 | GebràDer Sulzer Aktiengesellschaft | Process for the mechanical disintegration of cells |
| CN1265051A (en) * | 1997-06-24 | 2000-08-30 | 卡迪国际公司 | Method and appts. for lysing sludge and dividing solids in liquid suspension |
| CN103223365A (en) * | 2013-04-28 | 2013-07-31 | 王洪福 | Crusher with functions of impact crushing and extrusion shearing crushing |
| CN103343089A (en) * | 2013-07-10 | 2013-10-09 | 中国石油大学(华东) | Hydraulic cavitated cell wall disruption device |
| CN205288551U (en) * | 2016-01-21 | 2016-06-08 | 西安科技大学 | A high -efficient screening device of smashing for rock |
| CN205603585U (en) * | 2016-05-06 | 2016-09-28 | 上海唯科生物制药有限公司 | High pressure refiner |
| CN106501051A (en) * | 2016-12-17 | 2017-03-15 | 安徽普伦智能装备有限公司 | A kind of quick detection equipment processing meanss |
| CN208279604U (en) * | 2018-02-06 | 2018-12-25 | 江苏阿拉丁环保科技有限公司 | Microbial cell wall-breaking machine |
| CN211255928U (en) * | 2019-12-05 | 2020-08-14 | 大理德林海环保科技有限公司 | Totally enclosed blue alga pressurization broken wall equipment |
| CN211885500U (en) * | 2020-04-09 | 2020-11-10 | 厦门元之道生物科技有限公司 | Traditional chinese medicine broken wall extraction equipment |
| CN114292737A (en) * | 2022-01-07 | 2022-04-08 | 张立强 | Microbial cell wall breaking machine for biological medicine |
-
2018
- 2018-02-06 CN CN201810114722.8A patent/CN108102882A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4562972A (en) * | 1983-04-13 | 1986-01-07 | Kawasaki Jukogyo Kabushiki Kaisha | Micropulverizer |
| EP0303791A1 (en) * | 1987-08-21 | 1989-02-22 | GebràDer Sulzer Aktiengesellschaft | Process for the mechanical disintegration of cells |
| CN1265051A (en) * | 1997-06-24 | 2000-08-30 | 卡迪国际公司 | Method and appts. for lysing sludge and dividing solids in liquid suspension |
| CN103223365A (en) * | 2013-04-28 | 2013-07-31 | 王洪福 | Crusher with functions of impact crushing and extrusion shearing crushing |
| CN103343089A (en) * | 2013-07-10 | 2013-10-09 | 中国石油大学(华东) | Hydraulic cavitated cell wall disruption device |
| CN205288551U (en) * | 2016-01-21 | 2016-06-08 | 西安科技大学 | A high -efficient screening device of smashing for rock |
| CN205603585U (en) * | 2016-05-06 | 2016-09-28 | 上海唯科生物制药有限公司 | High pressure refiner |
| CN106501051A (en) * | 2016-12-17 | 2017-03-15 | 安徽普伦智能装备有限公司 | A kind of quick detection equipment processing meanss |
| CN208279604U (en) * | 2018-02-06 | 2018-12-25 | 江苏阿拉丁环保科技有限公司 | Microbial cell wall-breaking machine |
| CN211255928U (en) * | 2019-12-05 | 2020-08-14 | 大理德林海环保科技有限公司 | Totally enclosed blue alga pressurization broken wall equipment |
| CN211885500U (en) * | 2020-04-09 | 2020-11-10 | 厦门元之道生物科技有限公司 | Traditional chinese medicine broken wall extraction equipment |
| CN114292737A (en) * | 2022-01-07 | 2022-04-08 | 张立强 | Microbial cell wall breaking machine for biological medicine |
Non-Patent Citations (1)
| Title |
|---|
| 李玉英: "微生物生物技术", 中国农业大学出版社, pages: 264 - 265 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111606476A (en) * | 2020-05-26 | 2020-09-01 | 郑州众然供应链管理有限公司 | Microorganism inactivation device |
| CN113122439A (en) * | 2021-05-08 | 2021-07-16 | 王琼 | Device for extracting protein and nucleic acid by breaking wall of fungus and method for extracting protein and nucleic acid by applying device |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: Room 402 on the west side of Building B, No. 185 Jumao Street, Yuanhe Street, Xiangcheng District, Suzhou City, Jiangsu Province, 215000 Applicant after: JIANGSU ALADING ENVIRONMENTAL PROTECTION TECHNOLOGY CO.,LTD. Address before: 215000 No. 158 Chunshenhu East Road, Chengyang Street, Xiangcheng Economic and Technological Development Zone, Suzhou City, Jiangsu Province Applicant before: JIANGSU ALADING ENVIRONMENTAL PROTECTION TECHNOLOGY CO.,LTD. |
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| CB02 | Change of applicant information | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180601 |
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| WD01 | Invention patent application deemed withdrawn after publication |