CN108077882A - A kind of method that functional form seasoning syrup is prepared using biological fermentation process - Google Patents

A kind of method that functional form seasoning syrup is prepared using biological fermentation process Download PDF

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Publication number
CN108077882A
CN108077882A CN201711364738.6A CN201711364738A CN108077882A CN 108077882 A CN108077882 A CN 108077882A CN 201711364738 A CN201711364738 A CN 201711364738A CN 108077882 A CN108077882 A CN 108077882A
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China
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column
functional form
syrup
sugar
seasoning syrup
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CN201711364738.6A
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Inventor
韦武林
米运宏
李海利
陈贞
蓝伯广
蓝孙贵
韦启兵
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NANNING ZONGLIAN TECHNOLOGY Co Ltd
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NANNING ZONGLIAN TECHNOLOGY Co Ltd
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Priority to CN201711364738.6A priority Critical patent/CN108077882A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/125Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives containing carbohydrate syrups; containing sugars; containing sugar alcohols; containing starch hydrolysates
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/20Synthetic spices, flavouring agents or condiments
    • A23L27/24Synthetic spices, flavouring agents or condiments prepared by fermentation
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30Artificial sweetening agents
    • A23L27/31Artificial sweetening agents containing amino acids, nucleotides, peptides or derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L27/00Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30Artificial sweetening agents
    • A23L27/33Artificial sweetening agents containing sugars or derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention belongs to sugar refining fields, and in particular to a kind of method that functional form seasoning syrup is prepared using biological fermentation process is made of following material:Natural sugarcane juice, gold sand sugar, brown sugar, galactolipin, topaz Rice & peanut milk, fructus cannabis slurry, sucrose mutase, transfructosylase, trehalose synthetase, Arabinose isomerase, flavor yeast and lysozyme, more than material after the techniques such as staged fermentation, filtering, lysozyme degerming, concentration by being made seasoning syrup.Functional form seasoning syrup of the present invention has multiple efficacies, solve thes problems, such as that the effect of seasoning sugar product occurs in the market is single, product attendant disadvantages are more.Functional form syrup of the present invention is prepared using biological fermentation process, fermentation generates three kinds of isomaltoketose, fructooligosaccharide and Tagatose prebiotics factors, assign product prebiotics function factor, the present invention uses biological preservative, safety without health risk, this product also overcome dish after the culinary art of common syrup be hardened, wire drawing, easily discoloration the shortcomings of.

Description

A kind of method that functional form seasoning syrup is prepared using biological fermentation process
Technical field
The invention belongs to sugar refining fields, and in particular to a kind of to prepare functional form seasoning syrup using biological fermentation process Method.
Background technology
Flavouring is one of home cooking necessary article, there is acid, sweet tea, peppery etc., and white granulated sugar is one of flavoring agent of family's indispensability, It is mainly used for sweetener, colorant, corrigent, but is all that huge numbers of families are sold to crystal, except storage, convenient transportation Outside, use it is very inconvenient, it is bad it is quantitative, course of dissolution is longer, when especially preparing food, be easy to cause cauliflower au gratin and overdo or uneven. During as coloring, to dissolve and boil can just use, and be inconvenient to store after opening, the main easy moisture absorption.With development in science and technology, science Confirm that many such as obesities, hyperglycaemia, hypertension disease are all related with excessive edible sucrose.With constantly pushing away for intelligent society Increasingly pursue that facility, intelligence, health etc. are multi-functional to combine together into, the product that consumer eats.
For white granulated sugar liquid sugar for seasoning is developed for existing deficiency during family cooking, many scientific research personnel or enterprise Slurry, Chinese patent application:CN1666640A discloses a kind of single-purpose sugar for cooking, and raw material includes sweetener and tasty agents, sweet tea Contain honey element in taste agent, it, will be because of intake if consumer often eats the exceeded beverage or other food of happy cellulose content It is excessive to damage to the liver and nervous system of human body, old man particularly weaker to the ability of metabolism, pregnant woman, child Harm becomes apparent from;The saccharin sodium ingredient contained in sweetener does not also get consumer reception.In such as Chinese patent:CN103621866B A kind of liquid cooking sugar and preparation method thereof is disclosed, syrup is reconciled instead of liquid using the by-product in oligofructose production process State cooks the part component of syrup, adds a variety of natural sugars such as glucose, fructose, maltose, oligosaccharide etc., and product has and prevents Crystallization, while have oligofructose ingredient, add product function type.Although this production technology saves cost, production The by-product that the by-product that oligofructose generates particularly produces high-purity fructo oligosaccharides can dissociate to come containing chromatographic isolation resin Metal ion, have a certain impact to mouthfeel etc..
Sugar for seasoning is varied currently on the market, also the flavoring agent of functional type, but the function of these sugar for seasoning often cares for This loses that, and function is more single, there is also it is many defects of have it is to be solved.Therefore, it is more to research and develop a kind of effect, while can solve again The sugar for seasoning of many defects certainly occurred in the market is necessary.
The content of the invention
It is an object of the present invention to for above-mentioned sugar for seasoning effects many in the market are single, there are problems that number of drawbacks, It is various while the functional form seasoning syrup for the number of drawbacks that sugar for seasoning in the market occurs can be solved to provide a kind of effect.
In order to achieve the above objectives, the technical solution adopted in the present invention is:
A kind of method that functional form seasoning syrup is prepared using biological fermentation process, the seasoning syrup is mainly by following mass percent Substance be made:Natural sugarcane juice 40-45%, gold sand sugar 10-20%, brown sugar 12-15%, galactolipin 15-20%, topaz Rice & peanut milk 10-13% 2-3% is starched with fructus cannabis;The seasoning syrup also needs to 6 kinds of biological respinse auxiliary agents, 6 kinds of biological respinses during preparation Auxiliary agent and each account for gold sand sugar, brown sugar and galactolipin three's gross mass percentage are:Sucrose mutase 0.2-0.35%, fructose Based transferase 1-2.5%, trehalose synthetase 0.5-0.8%, Arabinose isomerase 0.1-0.15%, flavor yeast 2-4% and molten Bacterium enzyme 0.4-0.5%;The preparation method of the seasoning syrup is as follows:
(1)Weigh natural sugarcane juice, gold sand sugar, brown sugar, galactolipin, topaz Rice & peanut milk and fructus cannabis slurry respectively by mass percentage, it will More than material mixing, and it is 45-60% to be charged with pure water and adjust to weight percent shared by solid content, obtains compound Shape object;
(2)Weigh sucrose mutase, transfructosylase, trehalose synthetase, arabinose isomery respectively by mass percentage Enzyme, flavor yeast and lysozyme, and the A-B-C-D-E-F reaction columns being composed in series are added in, center pillar A adds in sucrose mutase, Column B adds in transfructosylase, and column C adds in trehalose synthetase, and column D adds in Arabinose isomerase, and column E adds in flavor ferment Mother, column F add in lysozyme;By step(1)Obtained mix paste passes sequentially through the A-B-C-D-E-F reaction columns being composed in series Microbe conversion is carried out, obtains zymotic fluid;
(3)By step(2)Obtained zymotic fluid is filtered, and is taken its filtrate, is analyzed it, it is desirable that indices are:Filter Liquid measures light transmittance >=80% with 722 type visible ray photometers under 720nm wavelength, and isomaltoketose is detected by GB1886.182 Content 15-23% scope, by GB/T23528 detection fructooligosaccharide content 20-40% scope, by GB/T23529 detect sea Algae sugared content 5-12% scope, by QB/T4613 detection Tagatose content 3-5% scope, by GB/T23530 flavor types Detect scope of the total nitrogen in 5-5.5%;Total plate count≤10 are detected by GB4789.23CFU/ml;
(4)Step will be met(3)The filtrate of indices is concentrated, and is charged with epsilon-polylysine, and being sufficiently stirred makes two Person is uniformly mixed, and obtains mixed system I, wherein, the epsilon-polylysine of addition and the mass ratio of filtrate are 50-80mg/kg;Then Mixed system I is concentrated into solid content and accounts for 75-80%, obtains mixed system II;
(5)By step(4)Obtained mixed system II pack up to functional form seasoning syrup
In the present invention, further instruction, the topaz Rice & peanut milk are made of a bar horse pearl yellow maize;Fructus cannabis slurry by bar Horse fructus cannabis is made.
In the present invention, further instruction, step(2)The A-B-C-D-E-F reaction columns being composed in series are fermenting The reaction condition of each reaction column is during conversion:It is 45-55 DEG C, PH 5.5-6.5, flow velocity 2-5L/min that column A, which controls temperature, column B controls temperature, and for 40-45 DEG C, PH 4.5-6.5, flow velocity 3-5L/min, column C controls temperature as 55-65 DEG C, PH 6.0-6.5, stream Speed is 2.5-4.5L/min, and column D controls temperature, and for 42-50 DEG C, PH 6.5-7.5, flow velocity 3-6L/min, column E controls temperature as 28- 34 DEG C, PH 5.0-5.5, flow velocity 3-6L/min, column F controls temperature as 35-40 DEG C, PH 5.2-5.5, flow velocity 3-5L/ min。
In the present invention, further instruction, step(2)Described in reaction column blade diameter length ratio be 1:8-1:12.
In the present invention, further instruction, step(4)The equipment used that concentrates is negative pressure dehydrator.
In the present invention, further instruction, step(5)Described in pack the amount often wrapped be 50-1000ml.
The present invention has the advantage that compared with prior art:
1. functional form seasoning syrup of the present invention has multiple efficacies, it is single to efficiently solve the effect of seasoning sugar product occurs in the market First, the problem of the defects of product is subsidiary is more.The functional form seasoning syrup of the present invention has the effect of a variety of prebioticses:Using sugarcane Normal juice, gold sand sugar, white granulated sugar generate isomaltoketose, fruit for raw material by sucrose mutase, transfructosylase catalysing sucrose Two kinds of prebiotics factors of oligosaccharides assign product prebiotics function factor, while preserve the nutritional ingredient in sugarcane, have made product both The nutritious and unsoundness factor overcomes pure white granulated sugar slurry, the nonfunctional of blending type syrup, glycemic index are high and should not excessively eat The shortcomings that using;Galactolipin is used as raw material, is got back a kind of prebiotics factor tower using Arabinose isomerase conversion galactolipin Lattice sugar so that this product tool improves product efficacy there are three types of the effect of prebiotics, while Tagatose is sweet taste low in calories always Agent, sugariness are the 92% of equivalent sucrose, and generated heat is only the 1/3 of sucrose, and synergy product sweetness overcomes product In containing fructooligosaccharide, isomaltoketose, trehalose sugariness reduce the shortcomings that, while reduce to a certain extent sugar heat. After testing, the GI values of product of the present invention are less than 30.Containing topaz Rice & peanut milk in this product material, by trehalose synthetase topaz In Rice & peanut milk partial starch conversion trehalose, trehalose can defencive function factor fructooligosaccharide do not decomposed in high temperature, sour environment, Its functional form is preserved, while trehalose plays an important role of the freshness and moisture of protecting dish.It is fermented using flavor yeast, the wind Taste yeast has the single digestion characteristics of substrate, only digests glucose and galactolipin, and few to other carbohydrates such as fructose, sucrose, fruit Sugar, trehalose, Tagatose etc. are without digestibility, therefore function flavor yeast has reaction not exclusively remaining grape in digestion dispensing Sugar and galactose metabolism product obtain flavor delicate flavour object, improve the delicate flavour of product.Functional form syrup of the present invention is sent out using biology Prepared by ferment method, fermentation generates the multiple functions type factor so that product of the present invention has multiple efficacies, solves sugar for seasoning in the market The characteristics of nonfunctional, single function.
2. the present invention utilizes the miscellaneous bacteria cell membrane of infection of the lysozyme digestion in preparation process, make miscellaneous bacteria that can not be metabolized life It grows, product working substance in high-temperature sterilization process is overcome to decompose, Maillard reaction occurs for carbohydrate, and product color is made to become brown etc. and is lacked Point.
3. product of the present invention uses biological preservative epsilon-polylysine, epsilon-polylysine resolves into human body human body The lysine of benefit to human body without health risk, with the preservatives such as potassium sorbate, sodium benzoate is used to compare, uses ε-poly- bad ammonia Sour biological preservative is to consumer without health risk.
The topaz Rice & peanut milk of this product invention is made for raw material using geography symbol product bar horse pearl yellow maize, there is Ba Mazhen Pearl yellow maize nutrition, at the same overcome dish after the culinary art of common syrup be hardened, wire drawing, easily discoloration the shortcomings of;Fructus cannabis slurry is adopted It is made of geography symbol product bar horse fructus cannabis, by microbe conversion, during fructus cannabis oil is soluble in the aqueous phase, changes single water and mix Fluidity matter prevents product crystallization to be precipitated, while adds product nutrition and product fragrance.
Specific embodiment
The present invention provides a kind of method that functional form seasoning syrup is prepared using biological fermentation process is provided, to make the present invention Purpose, technical solution and effect it is clearer, clear and definite, the present invention is described in more detail below.It should be appreciated that this place The specific embodiment of description is only used to explain the present invention, is not intended to limit the present invention.
Embodiment 1
It is provided by the invention it is a kind of functional form seasoning syrup is prepared using biological fermentation process method it is as follows:
A kind of method that functional form seasoning syrup is prepared using biological fermentation process, the seasoning syrup is mainly by following mass percent Substance be made:Natural sugarcane juice 40%, gold sand sugar 10%, brown sugar 12%, galactolipin 15%, topaz Rice & peanut milk 10% and fructus cannabis slurry 2%;It should Seasoning syrup also needs to 6 kinds of biological respinse auxiliary agents during preparation, 6 kinds of biological respinse auxiliary agents and each accounts for gold sand Sugar, brown sugar and galactolipin three's gross mass percentage are:Sucrose mutase 0.2%, transfructosylase 1%, trehalose synthetase 0.5%th, Arabinose isomerase 0.1%, flavor yeast 2% and lysozyme 0.4%;The preparation method of the seasoning syrup is as follows:
(1)Weigh natural sugarcane juice, gold sand sugar, brown sugar, galactolipin, topaz Rice & peanut milk and fructus cannabis slurry respectively by mass percentage, it will More than material mixing, and it is 45% to be charged with pure water and adjust to weight percent shared by solid content, obtains mixing paste Object;
(2)Weigh sucrose mutase, transfructosylase, trehalose synthetase, arabinose isomery respectively by mass percentage Enzyme, flavor yeast and lysozyme, and the A-B-C-D-E-F reaction columns being composed in series are added in, the blade diameter length ratio of the reaction column is 1: 8, center pillar A add in sucrose mutase, and column B adds in transfructosylase, and column C adds in trehalose synthetase, and column D adds in Arabic Sugared isomerase, column E add in flavor yeast, and column F adds in lysozyme;By step(1)Obtained mix paste passes sequentially through series connection The A-B-C-D-E-F reaction columns of composition carry out microbe conversion, wherein A-B-C-D-E-F reaction columns each reaction in microbe conversion The reaction condition of column is:Column A controls temperature, and for 45 DEG C, PH 5.5, flow velocity 2L/min, column B controls temperature as 40 DEG C, PH 4.5, Flow velocity is 3L/min, and column C controls temperature, and for 55 DEG C, PH 6.0, flow velocity 2.5L/min, column D controls temperature as 42 DEG C, PH 6.5, Flow velocity is 3L/min, and column E controls temperature, and for 28 DEG C, PH 5.0, flow velocity 3L/min, column F controls temperature as 35 DEG C, PH 5.2, stream Speed is 3L/min, and zymotic fluid is obtained after reaction;
(3)By step(2)Obtained zymotic fluid is filtered, and is taken its filtrate, is analyzed it, it is desirable that indices are:Filter Liquid measures light transmittance >=80% with 722 type visible ray photometers under 720nm wavelength, and isomaltoketose is detected by GB1886.182 Content is 15%, is 20% by GB/T23528 detection fructooligosaccharide contents, is 5% by GB/T23529 detection content of trehalose, by QB/ T4613 detection Tagatose contents are 3%, are 5% by GB/T23530 flavor types detection total nitrogen;Total plate count is detected by GB4789.2 ≤103CFU/ml;
(4)Step will be met(3)The filtrate of indices is concentrated, and is charged with epsilon-polylysine, and being sufficiently stirred makes two Person is uniformly mixed, and obtains mixed system I, wherein, the epsilon-polylysine of addition and the mass ratio of filtrate are 50mg/kg;Then will Mixed system I is concentrated into solid content with negative pressure dehydrator and accounts for 75%, obtains mixed system II;
(5)By step(4)Obtained mixed system II by the amount of every bag 50-1000ml pack up to functional form sugar for seasoning Slurry.
In the present embodiment, it is preferred that the topaz Rice & peanut milk is made of a bar horse pearl yellow maize;Fructus cannabis slurry by bar Horse fructus cannabis is made.
Embodiment 2
It is provided by the invention it is a kind of functional form seasoning syrup is prepared using biological fermentation process method it is as follows:
A kind of method that functional form seasoning syrup is prepared using biological fermentation process, the seasoning syrup is mainly by following mass percent Substance be made:Natural sugarcane juice 43%, gold sand sugar 15%, brown sugar 14%, galactolipin 17%, topaz Rice & peanut milk 12% and fructus cannabis slurry 2.5%; The seasoning syrup also needs to 6 kinds of biological respinse auxiliary agents during preparation, 6 kinds of biological respinse auxiliary agents and each accounts for gold Granulated sugar, brown sugar and galactolipin three's gross mass percentage are:Sucrose mutase 0.3%, transfructosylase 2%, trehalose synthesis Enzyme 0.6%, Arabinose isomerase 0.13%, flavor yeast 3% and lysozyme 0.45%;The preparation method of the seasoning syrup is as follows:
(1)Weigh natural sugarcane juice, gold sand sugar, brown sugar, galactolipin, topaz Rice & peanut milk and fructus cannabis slurry respectively by mass percentage, it will More than material mixing, and it is 53% to be charged with pure water and adjust to weight percent shared by solid content, obtains mixing paste Object;
(2)Weigh sucrose mutase, transfructosylase, trehalose synthetase, arabinose isomery respectively by mass percentage Enzyme, flavor yeast and lysozyme, and the A-B-C-D-E-F reaction columns being composed in series are added in, the blade diameter length ratio of the reaction column is 1: 10, center pillar A add in sucrose mutase, and column B adds in transfructosylase, and column C adds in trehalose synthetase, and column D adds in me The sugared isomerase of uncle, column E add in flavor yeast, and column F adds in lysozyme;By step(1)Obtained mix paste passes sequentially through string The A-B-C-D-E-F reaction columns of connection composition carry out microbe conversion, and wherein A-B-C-D-E-F reaction columns are each anti-in microbe conversion The reaction condition of Ying Zhu is:Column A controls temperature, and for 50 DEG C, PH 6, flow velocity 3L/min, column B controls temperature as 48 DEG C, PH 6, stream Fast is 4L/min, and column C controls temperature, and for 60 DEG C, PH 6.3, flow velocity 3.8L/min, it is 46 DEG C, PH 7 that column D, which controls temperature, flow velocity For 5L/min, column E controls temperature, and for 30 DEG C, PH 5.3, flow velocity 5L/min, it is 37 DEG C, PH 5.4 that column F, which controls temperature, and flow velocity is 4L/min obtains zymotic fluid after reaction;
(3)By step(2)Obtained zymotic fluid is filtered, and is taken its filtrate, is analyzed it, it is desirable that indices are:Filter Liquid measures light transmittance >=80% with 722 type visible ray photometers under 720nm wavelength, and isomaltoketose is detected by GB1886.182 Content is enclosed for 20%, is 30% by GB/T23528 detection fructooligosaccharide contents, is 9% by GB/T23529 detection content of trehalose, is pressed QB/T4613 detection Tagatose contents are 4%, are enclosed by GB/T23530 flavor types detection total nitrogen for 5.2%;Bacterium is detected by GB4789.2 Fall sum≤103CFU/ml;
(4)Step will be met(3)The filtrate of indices is concentrated, and is charged with epsilon-polylysine, and being sufficiently stirred makes two Person is uniformly mixed, and obtains mixed system I, wherein, the epsilon-polylysine of addition and the mass ratio of filtrate are 65mg/kg;Then will Mixed system I is concentrated into solid content with negative pressure dehydrator and accounts for 77%, obtains mixed system II;
(5)By step(4)Obtained mixed system II by the amount of every bag 50-1000ml pack up to functional form sugar for seasoning Slurry.
In the present embodiment, it is preferred that the topaz Rice & peanut milk is made of a bar horse pearl yellow maize;Fructus cannabis slurry by bar Horse fructus cannabis is made.
Embodiment 3
It is provided by the invention it is a kind of functional form seasoning syrup is prepared using biological fermentation process method it is as follows:
A kind of method that functional form seasoning syrup is prepared using biological fermentation process, the seasoning syrup is mainly by following mass percent Substance be made:Natural sugarcane juice 45%, gold sand sugar 20%, brown sugar 15%, galactolipin 20%, topaz Rice & peanut milk 13% and fructus cannabis slurry 3%;It should Seasoning syrup also needs to 6 kinds of biological respinse auxiliary agents during preparation, 6 kinds of biological respinse auxiliary agents and each accounts for gold sand Sugar, brown sugar and galactolipin three's gross mass percentage are:Sucrose mutase 0.35%, transfructosylase 2.5%, trehalose synthesis Enzyme 0.8%, Arabinose isomerase 0.15%, flavor yeast 4% and lysozyme 0.5%;The preparation method of the seasoning syrup is as follows:
(1)Weigh natural sugarcane juice, gold sand sugar, brown sugar, galactolipin, topaz Rice & peanut milk and fructus cannabis slurry respectively by mass percentage, it will More than material mixing, and it is 60% to be charged with pure water and adjust to weight percent shared by solid content, obtains mixing paste Object;
(2)Weigh sucrose mutase, transfructosylase, trehalose synthetase, arabinose isomery respectively by mass percentage Enzyme, flavor yeast and lysozyme, and the A-B-C-D-E-F reaction columns being composed in series are added in, the blade diameter length ratio of the reaction column is 1: 12, center pillar A add in sucrose mutase, and column B adds in transfructosylase, and column C adds in trehalose synthetase, and column D adds in me The sugared isomerase of uncle, column E add in flavor yeast, and column F adds in lysozyme;By step(1)Obtained mix paste passes sequentially through string The A-B-C-D-E-F reaction columns of connection composition carry out microbe conversion, and wherein A-B-C-D-E-F reaction columns are each anti-in microbe conversion The reaction condition of Ying Zhu is:Column A controls temperature, and for 55 DEG C, PH 6.5, flow velocity 5L/min, it is 45 DEG C that column B, which controls temperature, and PH is 6.5, flow velocity 5L/min, column C control temperature, and for 65 DEG C, PH 6.5, flow velocity 4.5L/min, it is 50 DEG C that column D, which controls temperature, and PH is 7.5, flow velocity 6L/min, column E control temperature, and for 34 DEG C, PH 5.5, flow velocity 6L/min, it is 40 DEG C that column F, which controls temperature, and PH is 5.5, flow velocity 5L/min obtain zymotic fluid after reaction;
(3)By step(2)Obtained zymotic fluid is filtered, and is taken its filtrate, is analyzed it, it is desirable that indices are:Filter Liquid measures light transmittance >=80% with 722 type visible ray photometers under 720nm wavelength, and isomaltoketose is detected by GB1886.182 Content is 23%, is 40% by GB/T23528 detection fructooligosaccharide contents, is 12% by GB/T23529 detection content of trehalose, by QB/ T4613 detection Tagatose contents are 5%, are 5.5% by GB/T23530 flavor types detection total nitrogen;It is total by GB4789.2 detection bacterium colonies Number≤103CFU/ml;
(4)Step will be met(3)The filtrate of indices is concentrated, and is charged with epsilon-polylysine, and being sufficiently stirred makes two Person is uniformly mixed, and obtains mixed system I, wherein, the epsilon-polylysine of addition and the mass ratio of filtrate are 80mg/kg;Then will Mixed system I is concentrated into solid content with negative pressure dehydrator and accounts for 80%, obtains mixed system II;
(5)By step(4)Obtained mixed system II by the amount of every bag 50-1000ml pack up to functional form sugar for seasoning Slurry.
In the present embodiment, it is preferred that the topaz Rice & peanut milk is made of a bar horse pearl yellow maize;Fructus cannabis slurry by bar Horse fructus cannabis is made.
Compliance test result
Functional form seasoning syrup of the present invention detects in preparation process and contains a certain amount of different wheat of the prebiotics factor in the product Bud ketose, fructooligosaccharide and Tagatose, the effect of imparting prebiotics of the present invention.In addition, present invention others are both effectiveness tests Card is as follows:
(1)Low glycemic index verification
Using two kinds of commercially available seasoning syrups of difference as control, its functional form seasoning syrup with 1-3 of the embodiment of the present invention is measured Glycemic index.Glycemic index(GI)Numerical value meaning refer to the food of the carbohydrate containing 50g with it is considerable amount of The percent value of glucose blood glucose response level in certain time body, reflection food raise the speed of blood glucose compared with glucose And ability.The GI values of usual glucose are decided to be 100.According to currently used GI value calculating methods, 30 healths are chosen The normal volunteer of situation foretaste experience, 6 one group of people, each 3 people of each group of men and women, wherein two groups as a control group, respectively For control group 1 and control group 2, allow each group of people person on an empty stomach 12 it is small when after, six groups of volunteers feed the carbohydrate containing 50g respectively Of the invention and commercially available syrup, measurement feed after 2 it is small when, survey everyone plasma glucose area under a curve respectively.Second My god, each group of people person on an empty stomach 12 it is small when after, feed the glucose of same aliquot, after measurement feed 2 it is small when, everyone plasma glucose Sugared area under a curve.It calculates the average Gui-value of every group of volunteer and is recorded in table.
Wherein, the calculation formula of GI values is:
GI=(The plasma glucose when gross area/equivalent glucose postprandial 2 is small under plasma glucose curve when test food postprandial 2 is small The gross area under sugared curve)×100
As a result such as following table:
Test group eats brown sugar Average Gui-value
Embodiment 1 24
Embodiment 2 27
Embodiment 3 25
Control group 1 64
Control group 68
From upper table data, the GI values of 1-3 of the embodiment of the present invention are below 30, and the GI of two groups of control groups is above 60, no It is suitble to eat more, is not particularly suitable for diabetes patient, data above confirms that seasoning syrup of the present invention compared with commercially available syrup, has The characteristics of low glycemic index.
(2)Other Evaluation of Functional of seasoning syrup
After finished product is made by the formula and step of embodiment 1-3, chooses volunteer and be investigated.
Edible object:Seasoning syrup made of embodiment 1-3 and 2 kinds of different commercially available seasoning syrups.
Eating method:As flavoring agent, the additive amount often eaten is for addition in the same style of cooking during daily breakfast, lunch and dinner culinary art 5ml, it is continuous 10 days edible.
Target of investication and study:Normal 100 people of physical condition is randomly divided into 5 groups, every group of 20 people, it is desirable that volunteer Corresponding seasoning syrup is evaluated after using 10 days.
Evaluation criterion:The freshness of dish is evaluated as evaluation point using after culinary art, be divided into favorable comment, in comment and difference is commented, Write down positive rating percentage;Whether dish changes colour and is evaluated as evaluation point using after culinary art, be divided into favorable comment, in comment and poor It comments, writes down positive rating percentage;Whether dish is hardened as evaluation point and is evaluated using after culinary art, be divided into favorable comment, in comment It is commented with difference, writes down positive rating percentage.Finding is as shown in the table:
Test group The freshness positive rating of dish Dish discoloration positive rating Dish is hardened situation positive rating
Embodiment 1 95% 90% 95%
Embodiment 2 95% 95% 90%
Embodiment 3 90% 95% 85%
Control group 1 45% 30% 25%
Control group 2 40% 25% 30%
Found out by upper table data, finished product made of embodiment 1-3 is through cooking freshness, dish discoloration and the dish of its dish The positive rating that the situation of being hardened obtains far more than two control groups, illustrates that functional form seasoning syrup of the present invention is substantially carried due to culinary art High delicate flavour, while effectively improve traditional seasoning syrup dish discoloration, the characteristics of being hardened after culinary art.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair Bright patent claim, the equal variation or modification change completed under the technical spirit suggested by all present invention, should all belong to Cover the scope of the claims in the present invention.

Claims (6)

  1. A kind of 1. method that functional form seasoning syrup is prepared using biological fermentation process, which is characterized in that the seasoning syrup mainly by The substance of following mass percent is made:Natural sugarcane juice 40-45%, gold sand sugar 10-20%, brown sugar 12-15%, galactolipin 15-20%, Topaz Rice & peanut milk 10-13% and fructus cannabis slurry 2-3%;The seasoning syrup also needs to 6 kinds of biological respinse auxiliary agents, institute during preparation It states 6 kinds of biological respinse auxiliary agents and each accounts for gold sand sugar, brown sugar and galactolipin three's gross mass percentage and be:Sucrose mutase 0.2-0.35%, transfructosylase 1-2.5%, trehalose synthetase 0.5-0.8%, Arabinose isomerase 0.1-0.15%, wind Taste yeast 2-4% and lysozyme 0.4-0.5%;The preparation method of the seasoning syrup is as follows:
    Natural sugarcane juice, gold sand sugar, brown sugar, galactolipin, topaz Rice & peanut milk and fructus cannabis slurry are weighed respectively by mass percentage, by more than Material mixing, and it is 45-60% to be charged with pure water and adjust to weight percent shared by solid content, obtains mix paste;
    Weigh respectively by mass percentage sucrose mutase, transfructosylase, trehalose synthetase, Arabinose isomerase, Flavor yeast and lysozyme, and the A-B-C-D-E-F reaction columns being composed in series are added in, center pillar A adds in sucrose mutase, column B Transfructosylase is added in, column C adds in trehalose synthetase, and column D adds in Arabinose isomerase, and column E adds in flavor yeast, column F adds in lysozyme;By step(1)Obtained mix paste passes sequentially through the A-B-C-D-E-F reaction columns being composed in series and carries out Microbe conversion obtains zymotic fluid;
    By step(2)Obtained zymotic fluid is filtered, and is taken its filtrate, is analyzed it, it is desirable that indices are:Filtrate is used 722 type visible ray photometers measure light transmittance >=80% under 720nm wavelength, and isomaltoketose content is detected by GB1886.182 In the scope of 15-23%, scope of the fructooligosaccharide content in 20-40% is detected by GB/T23528, trehalose is detected by GB/T23529 Content 5-12% scope, by QB/T4613 detection Tagatose content 3-5% scope, by GB/T23530 flavor types detect Total nitrogen is in the scope of 5-5.5%;Total plate count≤10 are detected by GB4789.23CFU/ml;
    Step will be met(3)The filtrate of indices is concentrated, and is charged with epsilon-polylysine, and being sufficiently stirred makes the two mixed It closes uniformly, obtains mixed system I, wherein, the epsilon-polylysine of addition and the mass ratio of filtrate are 50-80mg/kg;It then will be mixed Zoarium system I is concentrated into solid content and accounts for 75-80%, obtains mixed system II;
    By step(4)Obtained mixed system II pack up to functional form seasoning syrup.
  2. 2. a kind of method that functional form seasoning syrup is prepared using biological fermentation process according to claim 1, feature are existed In:The topaz Rice & peanut milk is made of a bar horse pearl yellow maize;The fructus cannabis slurry is made of a bar horse fructus cannabis.
  3. 3. a kind of method that functional form seasoning syrup is prepared using biological fermentation process according to claim 1, feature are existed In:Step(2)The A-B-C-D-E-F reaction columns being composed in series reaction condition of each reaction column in microbe conversion is: Column A controls temperature, and for 45-55 DEG C, PH 5.5-6.5, flow velocity 2-5L/min, column B controls temperature as 40-45 DEG C, PH 4.5-6.5, Flow velocity is 3-5L/min, and column C controls temperature as 55-65 DEG C, PH 6.0-6.5, flow velocity 2.5-4.5L/min, and column D controls the temperature to be 42-50 DEG C, PH 6.5-7.5, flow velocity 3-6L/min, column E controls temperature as 28-34 DEG C, PH 5.0-5.5, flow velocity 3-6L/ Min, column F control temperature as 35-40 DEG C, PH 5.2-5.5, flow velocity 3-5L/min.
  4. 4. a kind of method that functional form seasoning syrup is prepared using biological fermentation process according to claim 1, feature are existed In:Step(2)Described in reaction column blade diameter length ratio be 1:8-1:12.
  5. 5. a kind of method that functional form seasoning syrup is prepared using biological fermentation process according to claim 1, feature are existed In:Step(4)The equipment used that concentrates is negative pressure dehydrator.
  6. 6. a kind of method that functional form seasoning syrup is prepared using biological fermentation process according to claim 1, feature are existed In:Step(5)Described in pack the amount often wrapped be 50-1000ml.
CN201711364738.6A 2017-12-18 2017-12-18 A kind of method that functional form seasoning syrup is prepared using biological fermentation process Pending CN108077882A (en)

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CN116235893A (en) * 2021-12-07 2023-06-09 内蒙古蒙牛乳业(集团)股份有限公司 Semi-flowing slurry, preparation process and application thereof, and ice cream

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CN116235893A (en) * 2021-12-07 2023-06-09 内蒙古蒙牛乳业(集团)股份有限公司 Semi-flowing slurry, preparation process and application thereof, and ice cream

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Application publication date: 20180529