CN108077183A - A kind of animal risk taking behavior test device and its test method - Google Patents
A kind of animal risk taking behavior test device and its test method Download PDFInfo
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- CN108077183A CN108077183A CN201810083686.3A CN201810083686A CN108077183A CN 108077183 A CN108077183 A CN 108077183A CN 201810083686 A CN201810083686 A CN 201810083686A CN 108077183 A CN108077183 A CN 108077183A
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- 241001465754 Metazoa Species 0.000 title claims abstract description 59
- 238000012360 testing method Methods 0.000 title claims abstract description 37
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- 235000013305 food Nutrition 0.000 claims description 41
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- 238000011161 development Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
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- 206010034158 Pathological gambling Diseases 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention provides a kind of animal risk taking behavior test devices and preparation method thereof, belong to Animal Behavior Science research field, which includes：Babinet, the first simulator and the second simulator are provided in babinet, first simulator and the second simulator include the housing that side wall offers entrance, the first charging device and punishment device are provided in the housing of first simulator, the second charging device is provided in the housing of the second simulator.This test device and method, action selection of the animal for high risk situation and security situation is tested by a pair of of simulator, accurately the risk psychology change procedure of simulation animal, more being quantified to the risk taking behavior of mouse of science, the judge of science.
Description
Technical field
The present invention relates to Animal Behavior Science research field, in particular to a kind of animal risk taking behavior test device and
Its test method.
Background technology
Adventurous is a kind of conduct characteristics, refers to individual and tends to be engaged in the unascertainable behavior of some results.From positive meaning
It is adventurous that people is made to be more willing to receive severe challenge from the point of view of right way of conduct face, dare to exploring；But adventurous excessively high it may make people's
There is serious negative result in behavior, studies have found that, individual is adventurous higher, and injured possibility is bigger.Therefore, take a risk
Property a series of one of the excessively high feature for being counted as mental illnesses, such as pathological gambling, mostly dynamic obstacle.
It is shown from a social investigation, partially adventuresome crowd is concentrated mainly on teenager, since they are full of society
Curiosity is often liked attempting some fangles, and excessive risk taking behavior causes many teenagers to go astray, for example relates to
It is yellow, be involved in drug traffic, related to gambling activities etc..It can be seen that one of important skill that individual development needs obtain is to perceive and identify potential
Danger situation and excessive risk propensity can be avoided.From the perspective of scientific research, on adventurous at normal
Neuromechanism is discrepant between body and psychiatric patient, study adventuresome neuromechanism not only facilitate treatment it is some
Mental illness, while inspire meaning to the sound development of individual is maintained also to have.
In Neuscience basic research field, it is desirable to study a kind of morbid state firstly the need of establishing a kind of animal mould
Type simulates the emotion and behavioral activity of mankind's complexity by the mode of animal behavior normal form.Based on this, this patent prepares to establish
A kind of new animal risk taking behavior test system lower based on award driving.
The content of the invention
The first object of the present invention is to provide a kind of animal risk taking behavior test device, it is intended to pass through a pair of of simulator
It tests action selection of the animal for high risk situation and security situation, and then obtains a variety of for assessing animal risk
The parameter of sexual behaviour.
The second object of the present invention is to provide a kind of animal risk taking behavior test method, it is intended to be driven and be punished using award
Facility is penalized to test the risk taking behavior of animal, accurately simulates the risk psychology change procedure of animal, more science
The risk taking behavior of mouse is quantified, the judge of science.
In order to realize the above-mentioned purpose of the present invention, spy uses following technical scheme：
A kind of animal risk taking behavior test device including babinet, is provided with the first simulator and the second mould in babinet
Intend device, the first simulator and the second simulator include the housing that side wall offers entrance, the shell of the first simulator
The first charging device and punishment device are provided in vivo, and the second charging device is provided in the housing of the second simulator；
First charging device has the first storage bin for offering the first feeding port and for controlling the first feeding port
The first trigger device opened or closed, the second charging device is with the second storage bin equipped with the second feeding port and for controlling
The second trigger device opened or closed of the second feeding port is made, the bore of the first feeding port is more than the bore of the second feeding port.
Further, in preferred embodiments of the present invention, above-mentioned first trigger device and the second trigger device include：
Triggering block and the guide pad for being used to open or closing the first feeding port or the second feeding port, guide pad are articulated with housing by shaft
On, triggering block is slideably positioned in the bottom of housing, and guide pad is close to the side of triggering block and the corresponding position difference of triggering block
It is provided with the inclined-plane of mutual cooperation.
Further, in preferred embodiments of the present invention, slip is provided between the bottom of above-mentioned housing and triggering block
Mechanism, sliding equipment include holding section and sliding slot, and holding section is arranged at the bottom of triggering block, and sliding slot is arranged at the bottom of housing,
Holding section cooperates with sliding slot.
Further, in preferred embodiments of the present invention, above-mentioned sliding slot is fixedly installed bullet in one end away from entrance
Property component.
Further, in preferred embodiments of the present invention, above-mentioned punishment device includes pain stimulation element and is used for
The controller of control pain stimulation elements, pain stimulation element include being arranged at the metal contact piece of housing bottom, metal contact piece with
Controller is electrically connected.
Further, in preferred embodiments of the present invention, above-mentioned punishment device further includes visual stimulus element, vision thorn
Excimer part includes being arranged at the LED light of the bottom of the first storage bin, and LED light is electrically connected with controller.
Further, there is the beginning for being interconnected, setting gradually in preferred embodiments of the present invention, in above-mentioned babinet
Area, transition region and rewarding and punishing area, the first simulator and the second simulator are arranged at rewarding and punishing area.
Further, in preferred embodiments of the present invention, it is respectively provided at the top of above-mentioned beginning area, transition region and rewarding and punishing area
There is infrared camera.
A kind of test method of animal risk taking behavior, including：
Animal fasting is put into after one day in the babinet of the test device of any one of claim 1~8, and utilizes controller
There is selection to start punishment device while experimental animal obtains food；Utilize infrared camera observation and recording laboratory animal
Original active is recorded a video, and the event trace using video frequency tracking software records experimental animal in specific region, and then is recorded real
It tests animal and respectively enters the number of the first simulator and the second simulator, experimental animal in entrance first simulator
Or second before simulator in the residence time in transition region or rewarding and punishing area, induce the number of punishment and successfully obtain time of food
Number.
Further, in preferred embodiments of the present invention, above-mentioned punishment is 0.5~2s after animal obtains food
Interior, the probability for having 25~50% triggers 1~3s of visual stimulus and vola 1~3s of pain stimulation simultaneously, and visual stimulus is LED light
The alternate light warning of circle sample black and white sent, vola pain stimulation are foot shock.
Compared with prior art, beneficial effects of the present invention for example including：
This animal risk taking behavior test device provided by the invention, while it is provided with the first simulator and the second simulation
Device, wherein, in the first simulator first is triggered in experimental animal there is provided the first charging device and punishment device simultaneously
After charging device, certain probability triggering punishment device is had, and then certain punishment (such as pain that shocks by electricity can be received because of feed
Punishment and visual stimulus punishment)；Simultaneously as the bore of the first feeding port of the first charging device is more than the second charging device
The bore of second feeding port, after the first charging device is triggered, the food significantly larger than triggering second that experimental animal obtains feeds intake
Food after device.Therefore, the first simulator is simulation high risk, the device of high repayment Sexual behavior mode.Due to the second simulation
Punishment device is not provided in device, and the bore of the second feeding port is less than the bore of the first feeding port, therefore, the second simulation dress
It is set to the device for simulating low dangerous, low return Sexual behavior mode.The test device is by simulating both of these case, you can realization allows reality
The result differences that animal easily tells two kinds of selections are tested, so as to accelerate to the efficiency of animal risk taking behavior test experiments and accurate
Property.
The test method of this animal risk row provided by the invention, using above-mentioned test device, it is dynamic can to obtain experiment
Object respectively enters the number of the first simulator and the second simulator, experimental animal into first simulator or the
Before two simulators transition region or rewarding and punishing area residence time, induce punishment number and successfully obtain food number,
So as to accurately simulate the risk psychology change procedure of animal, more being quantified to the risk taking behavior of mouse of science,
The judge of science, test method is simple, and reproducibility is high.
Description of the drawings
It, below will be to required use in embodiment in order to illustrate more clearly of the technical solution of embodiment of the present invention
Attached drawing be briefly described, it should be understood that the following drawings illustrates only certain embodiments of the present invention, therefore is not to be seen as
It is the restriction to scope, it for those of ordinary skill in the art, without creative efforts, can be with root
Other relevant attached drawings are obtained according to these attached drawings.
Fig. 1 is the structure diagram for the animal risk taking behavior test device that embodiment of the present invention provides；
Fig. 2 is that the first simulator of the animal risk taking behavior test device that embodiment of the present invention provides is not triggering shape
Structure diagram under state；
Fig. 3 is the first simulator of the animal risk taking behavior test device that embodiment of the present invention provides in triggering state
Under structure diagram；
Fig. 4 is the second simulator of the animal risk taking behavior test device that embodiment of the present invention provides in triggering state
Under structure diagram.
Icon：100- animal risk taking behavior test devices；101- babinets；102- imports；103- infrared cameras；104- is opened
Beginning area；105- transition regions；106- rewarding and punishing area；107- railings；The first simulators of 108-；The second simulators of 109-；110- shells
Body；111- entrances；The first charging devices of 120-；The first storage bins of 121-；The first feeding ports of 122-；The first trigger devices of 123-；
The second charging devices of 130-；The second storage bins of 131-；The second feeding ports of 132-；The second trigger devices of 133-；134- triggering blocks；
135- guide pads；136- shafts；137- shielding surfaces；The first inclined-planes of 138-；The second inclined-planes of 139-；140- holding sections；141- sliding slots；
142- elastomeric elements；151- pain stimulation elements；152- visual stimulus elements.
Specific embodiment
To make the purpose, technical scheme and advantage of embodiment of the present invention clearer, implement below in conjunction with the present invention
The technical solution in embodiment of the present invention is clearly and completely described in attached drawing in mode, it is clear that described reality
The mode of applying is the embodiment of a part of embodiment of the present invention rather than whole.Based on the embodiment in the present invention, ability
The every other embodiment that domain those of ordinary skill is obtained without creative efforts, belongs to the present invention
The scope of protection.Therefore, below the detailed description of the embodiments of the present invention to providing in the accompanying drawings be not intended to limit will
The scope of the present invention of protection is sought, but is merely representative of the selected embodiment of the present invention.Based on the embodiment in the present invention,
The every other embodiment that those of ordinary skill in the art are obtained without creative efforts belongs to this
Invent the scope of protection.
In the description of the present invention, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", " on ", " under ", "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outer ", " up time
The orientation or position relationship of the instructions such as pin ", " counterclockwise " are based on orientation shown in the drawings or position relationship, are for only for ease of
The description present invention and simplified description rather than instruction imply that signified equipment or element must be with specific orientation, Yi Te
Fixed azimuth configuration and operation, therefore be not considered as limiting the invention.
In addition, term " first ", " second " are only used for description purpose, and it is not intended that instruction or hint relative importance
Or the implicit quantity for indicating indicated technical characteristic.Define " first " as a result, the feature of " second " can be expressed or
Implicitly include one or more this feature.In the description of the present invention, " multiple " are meant that two or more,
Unless otherwise specifically defined.
In the present invention, unless otherwise clearly defined and limited, term " installation ", " connected ", " connection ", " fixation " etc.
Term should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected or integral；Can be that machinery connects
It connects or is electrically connected；It can be directly connected, can also be indirectly connected by intermediary, can be in two elements
The connection in portion or the interaction relationship of two elements.It for the ordinary skill in the art, can be according to specific feelings
Condition understands the concrete meaning of above-mentioned term in the present invention.
In the present invention, unless otherwise clearly defined and limited, fisrt feature second feature it " on " or it " under "
It can be contacted directly including the first and second features, it is not to contact directly but pass through it that can also include the first and second features
Between other characterisation contact.Moreover, fisrt feature second feature " on ", " top " and " above " to include first special
Sign is directly over second feature and oblique upper or is merely representative of fisrt feature level height higher than second feature.Fisrt feature exists
Second feature " under ", " lower section " and " following " include fisrt feature immediately below second feature and obliquely downward or be merely representative of
Fisrt feature level height is less than second feature.
Embodiment 1, referring to figs. 1 to shown in Fig. 4
The present embodiment provides a kind of animal risk taking behavior test device 100, as shown in Figure 1, for being emitted to experimental animal
Dangerous behavior is tested, and is quantified with the risk taking behavior to experimental animal, the judge of science.
The animal risk taking behavior test device 100 includes babinet 101, and babinet 101 is hollow structure, internal to have for examination
The accommodation space of the free activity of animal is tested, in order to preferably simulate the simultaneously behavior of observation experiment animal and psychological condition, the babinet
Accommodation space in 101 is divided into three regions, that is, starts area 104, transition region 105 and rewarding and punishing area 106, these three regions mutually interconnect
Lead to and set gradually, any barrier can be not provided between each two region, such as only by 101 bottom setting-out mark of babinet
Show to distinguish；Can also set experimental animal can easily by barrier, such as railing 107 etc. is set.Meanwhile in order to
It observes and chase experiment animal is in the active situation in these three regions, be both provided with infrared camera at the top of these three regions
103；The infrared camera 103 connects computer simultaneously, and the audio video synchronization recorded is transmitted in computer, is chased after using video
Track software can analyze the behavior of experimental animal in real time.
In these three regions, start area 104, close to the import 102 of babinet 101, experimental animal has just been placed into babinet 101
When middle, that is, starting area 104；Rewarding and punishing area 106 is the one side away from babinet 101 import 102, be arranged inside being useful for testing
The first simulator 108 and the second simulator 109 of animal risk taking behavior, enter rewarding and punishing area 106 by recording experimental animal
Number and the time, can reaction test animal risk taking behavior ability；Transition region 105 positioned at the middle part of babinet 101, that is, is located at
Start between area 104 and rewarding and punishing area 106, by recording experimental animal in 105 residence time of transition region (i.e. into rewarding and punishing area
106 incubation period), you can it goes to take a risk from a kind of trial of side reflection experimental animal and worries the psychological shape of penalized complexity
State.
First simulator 108 (as shown in Figures 2 and 3) and the second simulator 109 (as shown in Figure 4) are to aim at mould
Intend the device that animal risk taking behavior is set, including housing 110, the side wall of housing 110 is offered entrance 111 and moved with simulated experiment
The habit that object (such as mouse) love is got into the cave.In both simulators, the first simulator 108 is for simulating high-risk, height
The scene of return；Second simulator 109 is for simulating the scene of low dangerous, low return.First simulator, 108 He
Second simulator 109 is respectively provided with charging device, i.e. the first charging device 120 and the second charging device 130, and difference exists
In under same trigger condition, the first charging device 120 is triggered, and the food that experimental animal obtains is significantly larger than the second throwing
Expect that device 130 is being triggered as a result, i.e. high repayment.In addition, punishment device is additionally provided in the first simulator 108, for giving
The experimental animal for obtaining food applies punishment (such as the punishment of electric shock pain or visual stimulus punishment), i.e. high-risk.
The concrete structure of first charging device 120 and the second charging device 130 is as follows：
As shown in Figures 2 and 3, the first charging device 120 include the first storage bin 121 and the first trigger device 123, first
The side wall of storage bin 121 offers the first feeding port 122, and the first trigger device 123 is arranged at the lower part of the first storage bin 121 simultaneously
It is used to open or closes the first feeding port 122.
As shown in figure 4, the second charging device 130 includes the second storage bin 131 and the second trigger device 133, the second storing
The side wall in storehouse 131 offers the second feeding port 132, and the second trigger device 133 is arranged at the lower part of the second storage bin 131 and is used for
Open or close the second feeding port 132.
First trigger device 123 and the second trigger device 133 include：
Triggering block 134 is correspondingly arranged with the entrance 111 of housing 110, is set between the bottom of triggering block 134 and housing 110
There is sliding equipment, triggering block 134 is slideably positioned in the bottom of housing 110 by sliding equipment.Sliding equipment includes holding section 140
With sliding slot 141, holding section 140 is arranged at the bottom of triggering block 134, and sliding slot 141 is arranged at the bottom of housing 110, holding section 140
It cooperates with sliding slot 141.Meanwhile sliding slot 141 is fixedly installed elastomeric element in one end of the entrance 111 away from housing 110
142。
Guide pad 135 is located at the first storage bin 121 (or second storage bin 131) between triggering block 134, and is oriented to
Block 135 is articulated with by shaft 136 on housing 110, under the effect of external force, can be rotated with around the shaft 136.Guide pad
135 have shielding surface 137, and shielding surface 137 is located at close to the position at 135 edge of guide pad, which is used to open or closes
Close the first feeding port 122 or the second feeding port 132.
Guide pad 135 is being the first inclined-plane 138 close to the side of triggering block 134；Triggering block 134 with 135 phase of guide pad
To position be equipped with the second inclined-plane 139, the first inclined-plane 138 and the second inclined-plane 139 cooperate.Its trigger theory is：Work as experiment
After animal pierces housing 110, it can promote triggering block 134 relatively to 110 basal sliding of housing, in the process that triggering block 134 is slided
In, the first inclined-plane 138, which can be contacted gradually with the second inclined-plane 139 and pass through the active force between inclined-plane, promotes guide pad 135 to turn
It is rotated centered on axis 136, so that the shielding surface 137 on guide pad 135 is gradually distance from the first throwing of the first storage bin 121
Material mouth 122 (or second feeding port 132 of the second storage bin 131), so as to open the first feeding port 122 (or the second feeding port
132)；It, can be in elastomeric element when triggering block 134 slides the one end for the elastomeric element 142 for moving closer to be set in sliding slot 141
It is moved under the action of 142 to negative direction, and then closes the first feeding port 122 (or second feeding port 132), complete triggering feeding
Journey.
In order to which the feeding amount between the first simulator 108 and the second simulator 109 is made to have significant difference, first throws
Expect that the bore of the first feeding port 122 of device 120 is more than the bore of the second feeding port 132 of the second charging device 130, Jin Ershi
Existing, after the first charging device 120 is triggered, the food launched is much larger than the food that the second charging device 130 is launched, that is, tests
It is high that animal triggers the food return that the first charging device 120 is obtained.Such as in the present embodiment, the first storage bin 121 and
The food of granular is placed in two storage bins 131, and the food particle in the first storage bin 121 is big, second
Food particle in storage bin 131 is small.After experimental animal triggers the first charging device 120,1 bulky grain sample is fallen
Food；And after experimental animal triggers the second charging device 130, the food of 1 little particle sample can be fallen.In addition,
To food device experimental animal is needed actively to go to inhale to permit thick food in traditional experiment, this is in itself to laboratory breeding
The process (because experimental animal gets used to food) of a study is needed for animal；And this thick food is difficult to essence
Really control quantity of food, and be susceptible to feeding tube road and block risk.And the food for this granular that the present embodiment uses, simulation
The food that experimental animal usually eats, and slid after experimental animal is triggered to food organ, experimental animal is allowed to obtain big
The small food not waited.
The punishment device set in first simulator 108 includes pain stimulation element 151 and controller (not shown), should
Controller is used to start pain stimulation element 151 and controls the action frequency of the pain stimulation element 151.In the present embodiment,
Pain stimulation element 151 includes the metal contact piece for being arranged at 110 bottom of housing, which is electrically connected with controller.When
Experimental animal is carried out in the first simulator 108, and vola is contacted with metal contact piece, after controller sends electric shock instruction
(after i.e. metal contact piece is powered), experimental animal can be subject to foot shock to punish.
In the present embodiment, punishment device further includes visual stimulus element 152, which includes setting
LED light in the bottom of the first storage bin 121, LED light are electrically connected with controller.Controller is used to start pain stimulation simultaneously
Element 151 and visual stimulus element 152 and the action frequency for controlling the pain stimulation element 151 and visual stimulus element 152.
Punishment device action be：When experimental animal is same into the probability in the first simulator 108, having 25~50%
When trigger 1~3s of visual stimulus and vola 1~3s of pain stimulation, i.e., experimental animal vola and energization 1~3s metal contact piece connect
It touches, foot shock can be subject to punish；Simultaneously storage bin bottom LED can also send the alternate light warning 1 of circle sample black and white~
3s, this alternate light warning of circle sample black and white can give experimental animal (such as mouse) is a kind of visually to simulate eagle capture
Scene make its generate the feared state of mind (because one of natural enemy of mouse is exactly eagle, mouse see this scape can not by fear, this
Kind fear is inborn).Therefore, which employs vision and pain stimulation and is arranged as more severe punishment simultaneously
It applies, experimental animal is really allowed to feel exact adventurous and experimental animal can be made to pay spirit and the human body because of food desire
On cost.
In contrast, traditional test device is inadequate to the punishment of experimental animal, and punishment as defined in it simply allows experiment dynamic
Object waits longer time to obtain food again, does not play the role of substantive punishment but, and experimental animal still can be in order to full
Sufficient single obtains the desire of bigger food and the device of its preference selection high risk and high reward is promoted (would rather to wait longer
Time will also seek the desire of bigger food acquisition).
The present embodiment also provides a kind of test method of animal risk taking behavior, is specially：
Step a：Animal fasting is put into the babinet 101 of above-mentioned test device after one day, allows its freely activity, and utilizing
Controller selectively starts punishment device while experimental animal obtains food.
Further, above-mentioned punishment is in 0.5~2s after experimental animal obtains food, has 25~50% probability
Trigger 1~3s of visual stimulus and vola 1~3s of pain stimulation simultaneously.Wherein, visual stimulus is the circle sample black and white that LED light is sent
Alternate light warning, vola pain stimulation are foot shock.
Step b：It is observed using infrared camera 103 and records the original active video recording of animal, and it is soft using video frequency tracking
Event trace of the part recording laboratory animal in specific region, and then recording laboratory animal respectively enters the first simulator 108
The number of number, induction punishment with the second simulator 109 and the number for successfully obtaining food.
The experimentation is：
Drink is prohibited in the mouse fasting of a whole day on daytime, 6 points mouse is put at night in babinet 101.It is at night enlivening for mouse
Time, and mouse can more promote its search of food because one day hungry.Constantly it is familiar in babinet 101 by mouse
After exploration, viral marketing is attempted into the simulator in rewarding and punishing area 106 (because mouse is liked exploring and innately likes getting into the cave).Such as
Fruit mouse enters the first simulator 108 of high risk and high reward, and the probability for just having 25~50% can trigger punishment device, such as
Fruit does not have penalized, and mouse will obtain bigger granular food.After once triggering is punished, that is, mouse obtains food
0.5~2s can trigger foot shock simultaneously and stimulate the LED light vision 1~3s of fear on 1~3s and the crown, i.e., when mouse is being enjoyed
By by the attack of terrorism, viral marketing is escaped in a panic at this time when cuisines.And since the second simulator 109 is not provided with punishing
Device is penalized, mouse will obtain fewer granular food on triggering.
By late 6 points of records to next day (12h), pass through computer and camera shooting, event trace analysis system, electricity at 6 points
Hit, vision fear number record, successfully obtain the parameters such as food number record, for example, mouse into the first simulator 108 or
The number of second simulator 109 successfully triggers the number that organ obtains the number of food, induction is punished, and then reflects mouse
Risk taking behavior.Mouse enters the incubation period before rewarding and punishing area 106, and (i.e. experimental animal is for the first time into rewarding and punishing area 106 in transition region 105
Residence time) and into the number in rewarding and punishing area 106 and time aid in wish power that reflection mouse takes the plunge etc..It is above-mentioned
Aggregation of data gets up analysis can more being quantified to the risk taking behavior of mouse of science, the judge of science.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from the present invention's
Many other change and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of animal risk taking behavior test device, which is characterized in that it includes babinet, and the first simulation is provided in the babinet
Device and the second simulator, first simulator and second simulator include the shell that side wall offers entrance
Body is provided with the first charging device and punishment device, second simulator in the housing of first simulator
Housing in be provided with the second charging device；
First charging device, which has, offers the first storage bin of the first feeding port and for controlling described first to feed intake
Mouthful the first trigger device opened or closed, second charging device have equipped with the second feeding port the second storage bin with
And the second trigger device opened or closed for controlling second feeding port, the bore of first feeding port are more than institute
State the bore of the second feeding port.
2. animal risk taking behavior test device according to claim 1, which is characterized in that first trigger device and institute
Stating the second trigger device includes：Triggering block and it is used to open or closes leading for first feeding port or second feeding port
To block, the guide pad is articulated with by shaft on the housing, and the triggering block is slideably positioned in the bottom of the housing, institute
State the inclined-plane that guide pad is respectively arranged with mutual cooperation close to the side of the triggering block and the corresponding position of the triggering block.
3. animal risk taking behavior test device according to claim 2, which is characterized in that the bottom of the housing with it is described
Sliding equipment is provided between triggering block, the sliding equipment includes holding section and sliding slot, and the holding section is arranged at described touch
The bottom of block is sent out, the sliding slot is arranged at the bottom of the housing, and the holding section cooperates with the sliding slot.
4. animal risk taking behavior test device according to claim 3, which is characterized in that the sliding slot is entering away from described in
One end of mouth is fixedly installed elastomeric element.
5. animal risk taking behavior test device according to claim 1, which is characterized in that the punishment device includes pain
Stimulation elements and the controller for controlling the pain stimulation element, the pain stimulation element include being arranged at the shell
The metal contact piece of body bottom, the metal contact piece are electrically connected with the controller.
6. animal risk taking behavior test device according to claim 5, which is characterized in that the punishment device, which further includes, to be regarded
Feel stimulation elements, the visual stimulus element include be arranged at first storage bin bottom LED light, the LED light with
The controller is electrically connected.
7. animal risk taking behavior test device according to claim 1, which is characterized in that have in the babinet and mutually interconnect
Beginning area, transition region and the rewarding and punishing area lead to, set gradually, first simulator and the second simulator are arranged at the prize
Penalize area.
8. animal risk taking behavior test device according to claim 7, which is characterized in that the beginning area, the transition
Infrared camera is both provided at the top of area and the rewarding and punishing area.
9. a kind of test method of animal risk taking behavior, which is characterized in that it includes：
Experimental animal fasting is put into the babinet of claim 1~8 any one of them test device after one day, and utilizes control
Device processed has selection to start punishment device while experimental animal obtains food；It is observed using infrared camera and records experiment and moved
The original active video recording of object, and the event trace using video frequency tracking software records experimental animal in specific region, Jin Erji
Record experimental animal respectively enters the number of the first simulator and the second simulator, experimental animal is simulated into described first
Before device or the second simulator transition region or rewarding and punishing area residence time, induce punishment number and successfully obtain food
Number.
10. the test method of animal risk taking behavior according to claim 9, which is characterized in that the punishment is when described
Experimental animal is obtained in 0.5~2s after food, and the probability for having 25~50% triggers 1~3s of visual stimulus and vola pain simultaneously
Pain stimulates 1~3s, and the visual stimulus is the alternate light warning of circle sample black and white that LED light is sent, the vola pain thorn
Swash for foot shock.
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| CN112005961A (en) * | 2020-07-22 | 2020-12-01 | 北京农业智能装备技术研究中心 | Automatic change condition position preference class animal behavior experimental apparatus |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009131175A (en) * | 2007-11-29 | 2009-06-18 | Univ Of Tokyo | Device and method for evaluating behavioral propensity |
| CN106889022A (en) * | 2017-01-17 | 2017-06-27 | 南昌大学 | Rat risk chooses the training method of behavior |
| CN206558098U (en) * | 2017-01-17 | 2017-10-13 | 南昌大学 | A kind of behavioral pharmacology research device of rat risk choice |
| CN207784033U (en) * | 2018-01-29 | 2018-08-31 | 王存金 | A kind of animal risk taking behavior test device |
-
2018
- 2018-01-29 CN CN201810083686.3A patent/CN108077183B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2009131175A (en) * | 2007-11-29 | 2009-06-18 | Univ Of Tokyo | Device and method for evaluating behavioral propensity |
| CN106889022A (en) * | 2017-01-17 | 2017-06-27 | 南昌大学 | Rat risk chooses the training method of behavior |
| CN206558098U (en) * | 2017-01-17 | 2017-10-13 | 南昌大学 | A kind of behavioral pharmacology research device of rat risk choice |
| CN207784033U (en) * | 2018-01-29 | 2018-08-31 | 王存金 | A kind of animal risk taking behavior test device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112005961A (en) * | 2020-07-22 | 2020-12-01 | 北京农业智能装备技术研究中心 | Automatic change condition position preference class animal behavior experimental apparatus |
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