CN106889022A - Rat risk chooses the training method of behavior - Google Patents
Rat risk chooses the training method of behavior Download PDFInfo
- Publication number
- CN106889022A CN106889022A CN201710030684.3A CN201710030684A CN106889022A CN 106889022 A CN106889022 A CN 106889022A CN 201710030684 A CN201710030684 A CN 201710030684A CN 106889022 A CN106889022 A CN 106889022A
- Authority
- CN
- China
- Prior art keywords
- training
- selection
- animal
- experimental animal
- reward
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000012549 training Methods 0.000 title claims abstract description 228
- 238000000034 method Methods 0.000 title claims abstract description 28
- 238000010171 animal model Methods 0.000 claims abstract description 171
- 241001465754 Metazoa Species 0.000 claims abstract description 91
- 230000006399 behavior Effects 0.000 claims abstract description 40
- 238000001514 detection method Methods 0.000 claims abstract description 22
- 230000006978 adaptation Effects 0.000 claims abstract description 4
- 230000005056 memory consolidation Effects 0.000 claims abstract description 4
- 238000002474 experimental method Methods 0.000 claims description 28
- 238000012360 testing method Methods 0.000 claims description 13
- 235000013305 food Nutrition 0.000 claims description 11
- 238000012544 monitoring process Methods 0.000 claims description 10
- 238000013401 experimental design Methods 0.000 claims description 9
- 230000000153 supplemental effect Effects 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 230000004069 differentiation Effects 0.000 claims description 8
- 230000003542 behavioural effect Effects 0.000 claims description 6
- 235000005911 diet Nutrition 0.000 claims description 6
- 230000037213 diet Effects 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 241000894007 species Species 0.000 claims description 6
- 230000006641 stabilisation Effects 0.000 claims description 6
- 238000011105 stabilization Methods 0.000 claims description 6
- 238000012937 correction Methods 0.000 claims description 5
- 238000011160 research Methods 0.000 claims description 4
- 238000012163 sequencing technique Methods 0.000 claims description 4
- 208000019901 Anxiety disease Diseases 0.000 claims description 3
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 3
- 241001553178 Arachis glabrata Species 0.000 claims description 3
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 3
- 235000018262 Arachis monticola Nutrition 0.000 claims description 3
- 206010021703 Indifference Diseases 0.000 claims description 3
- 230000009471 action Effects 0.000 claims description 3
- 230000036506 anxiety Effects 0.000 claims description 3
- 230000000903 blocking effect Effects 0.000 claims description 3
- 230000001143 conditioned effect Effects 0.000 claims description 3
- 230000007423 decrease Effects 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 235000015203 fruit juice Nutrition 0.000 claims description 3
- 238000004900 laundering Methods 0.000 claims description 3
- 230000036651 mood Effects 0.000 claims description 3
- 235000020232 peanut Nutrition 0.000 claims description 3
- 230000011514 reflex Effects 0.000 claims description 3
- 238000007619 statistical method Methods 0.000 claims description 3
- 206010000117 Abnormal behaviour Diseases 0.000 claims 1
- 230000037396 body weight Effects 0.000 claims 1
- 238000012790 confirmation Methods 0.000 claims 1
- 230000008450 motivation Effects 0.000 claims 1
- 238000013461 design Methods 0.000 abstract description 3
- 210000000038 chest Anatomy 0.000 description 21
- 210000004556 brain Anatomy 0.000 description 3
- 230000008901 benefit Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 208000001613 Gambling Diseases 0.000 description 1
- 208000012902 Nervous system disease Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000003924 mental process Effects 0.000 description 1
- 230000004007 neuromodulation Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K15/00—Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
- A01K15/02—Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
- A01K15/027—Exercising equipment, e.g. tread mills, carousels
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Physical Education & Sports Medicine (AREA)
- Eye Examination Apparatus (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
Abstract
The invention discloses the training method that a kind of rat risk chooses behavior, comprise the following steps：Step one, trainer prepare；Step 2, experimental animal prepare；Step 3, scene adaptation training；Step 4, preference detection and amendment training；Step 5, assignment training；Step 6, memory consolidation training；Step 7, risk choice training.Training method of the invention and step design are more reasonable, more scientific, can significantly reduce the training time, and training for promotion efficiency, training has higher success rate, and can obtain true and reliable experimental data, contributes to researcher to be efficiently monitored to animal behavior.
Description
Technical field
The present invention relates to the association area of pharmaceutical research, and in particular to a kind of rat risk chooses the training side of behavior
Method.
Background technology
Behavior is chosen（Decision Making）It is common mental process in daily life.Wherein, risk choice is to pick out
The person of selecting judges to income condition obtained by choice object and the probability being likely to occur, and costs and benefits are weighed,
So as to the behavior for making one's choice.Risk choice behavior is closely bound up with daily life, and the behavior, disorderly meeting was to individual, family
Front yard or even society produce and have a strong impact on, and it is a typical example to be addicted to gambling.In addition, some the nervous system diseases
The disorder of individual choice behavior can be caused.Therefore, the neuromodulation mechanism of research risk choice behavior is conducive to finding alleviation disease
The ailing basic method of people, the quality of life to improving patient is significant.
Training method its science for the rat risk choice behavior for using at present has been short of, pertinent arts basis
Shortcoming, training effectiveness is relatively low.Therefore the training method that more science, efficient rat risk choose behavior is designed, is more helped
Yu researcher is efficiently monitored to animal behavior, and preferably the related brain areas for regulation and control risk choice behavior launch to grind
Study carefully work.
The content of the invention
The purpose of the present invention is directed to problem above, there is provided a kind of rat risk chooses the training method of behavior, wherein accumulateing
Knowwhy containing relevant speciality in the ambits such as Animal Behavior Science, behavioral pharmacology, Neurobiology so that training method
It is more reasonable, more scientific, the related brain areas of regulation and control risk choice behavior in behavior task is more precisely talked about, reduce other uncorrelated brains
The influence that area's active belt comes, contributes to training for promotion efficiency.
The present invention is achieved through the following technical solutions, and rat risk chooses the training method of behavior, it is characterised in that its
The step of training method, is as follows：
Step one, trainer prepare：Trainer will at least include training box, animal triggering device, selection arm, sound feedback
System, reward area, monitoring and record system；（1）Multiple training chest can be set in the training box of setting；（2）The training of training box
Animal triggering device is set, and experimental animal is required for touch trigger device before starting each training mission, it is ensured that experiment exists in room
Carried out on the premise of experimental animal operant conditioned reflex；（3）Two profiles, structure equal indifferences are set in the training chest of training box
Different selection arm, selects the species of arm not limit, the passage and depressible action bars passed through for experimental animal.Each selection
Independent mux --out signal exhibits area is set on the structure of arm or in surrounding space certain limit, is easy to experimental animal to set up signal with phase
The contact between arm should be selected.Signal can be diversified forms, but need to ensure the signal influence such as nonirritant on experimental animal.
Signal is divided into two kinds in the training mission, using the differentiation of figure or the differentiation of color or the differentiation conduct of bright light number
The two species diversity signals that experimental animal can differentiate；（4）Sound feedback system is set in training box, according to the selection of experimental animal
Situation gives the prompting of certain sound feedback alternatively situation, such as the positive tone for raising up is set into correct selection carries
Show sound, the passive tone of decline is set to wrong choice prompt tone；（5）Reward area is set in the training chest of training box, only in fact
Testing animal arrival reward area can obtain quantitative reward.Bonuses could be arranged to peanut, water, fruit juice according to experimental program
Etc. the food that can transfer experimental animal enthusiasm.According to task, reward is divided into two kinds, a kind of for reward probability is 100% to determine
Small reward is measured, reward S is named as, the amount of reward S is depending on experimental design.Another kind is quantitative less than 100% for reward probability
Big reward, is named as reward L, and the reward probability and reward amount for rewarding L can be depending on experimental designs；（6）Set in training box
Monitoring and record system, it is ensured that experimental animal state can in real time be monitored and record, operating time of the record key element including animal,
Selection situation, reward situation, feedback etc..
Step 2, experimental animal prepare：Experimental animal trains first 1 week by handle treatment, experimental animal is familiar with instruction
Practice personnel, exclude animal influences training effect because producing the moods such as fear, anxiety on experimenter；Sound is carried out after Handle
Amount strength test, the wave volume should ensure that experimental animal will not produce the exception row such as fear in the case of can perceiving sound
For record source of sound is away from the parameter such as experimental animal distance and decibels size, correct prompt tone and mistake in implementing for experiment
The parameter that prompt tone is set；Testing first three day need to carry out control water or control food preparation according to experimental design, keep the weight of animals not low
The 85% of normal type before food is controlled, it is ensured that make animal produce the dynamic of operation task in the case where animal bodies situation is not damaged
Machine.During the training period, after training makes the free diet of experimental animal to no longer diet after terminating every time, continue to control water or control food.Respectively
Can start formal training after the completion of item preparation.
Step 3, scene adaptation training：Experimental animal is put into the training chest original position in training box, makes it freely visit
Rope, once animal touch trigger device, then start a training mission, scene laundering period purpose is experimental animal is familiar with instruction
Practice place and its surrounding enviroment, understand training box structure, the award mode of familiarization training case and feature etc., therefore need not in training chest
Any signal is set, as long as experimental animal selects any selection arm after triggering device is started and reaches reward region, is sounded
Correct prompt tone simultaneously gives certain reward.If experimental animal repeats, multi-pass operation or faulty operation, mistake of sounding is carried
Show sound, given without reward.Each training mission is both needed to the autonomous touch trigger device of experimental animal to start.The behaviour of experimental animal
Make situation and reward situation is both needed to record, be easy to subsequently carry out statistical analysis to behavior task operating situation.Experiment number foundation
Reward is set depending on the situation of size and experimental animal, prevents influence animal behavior of overtraining.When experimental animal operation just
True rate reach more than 80% and it is continual and steady after think that experimental animal has realized training objective, turn to the preference profile to animal
Detected and corrected.
Step 4, preference detection and amendment training：Statistical experiment animal selects two to select arm in training box respectively
Number of times, compares whether they have notable difference, if not existing difference, then it is assumed that experimental animal selects a certain item in the absence of preference
Situation, without being modified training；If having differences, then it is assumed that experimental animal has the situation that preference selects a certain item.In order to
Prevent the study and selection of this preference influence animal in training below and experiment of experimental animal, the experiment for having preference from moving
Thing need to carry out preference amendment training.
It is every in training box using preference amendment training method for the rat for having preference in preference amendment training
Signal is not provided with individual selection arm, experimental animal unrestricted choice could be obtained when only successively selecting variant twice in reward area
Obtain and necessarily reward.For this regular experimental animal without law society, then increase supplemental training, i.e., selected by artificial side of blocking
The mode of arm helps the rat to carry out the training by selecting arm, per 4~6 freedom of entry selection mode detections again afterwards of supplemental training
The selection situation of experimental animal, for two selections of selection arm in the case of experimental animal association alternate selection or unrestricted choice
When number of times is without significant difference, it is believed that experimental animal is without preference.
Step 5, assignment training：Assignment training is divided into secure item training and risk training, two priorities of tasking learning
Order is random, and the study of the next item down task could be turned in the case of must ensureing a task association.Signal kinds and reward kind
Class need to remain matching.The selection situation of daily experimental animal is counted, when experimental animal selection is provided with the choosing of corresponding signal
The frequency for selecting arm reaches more than 80% in continuous 4 days, that is, select accuracy continuous 4 days up to more than 80%, then it is assumed that it has learned this
Task, reaches training objective.（1）Secure item training is that experimental animal is put into training box original position, if training box sets many
Individual training chest, then experimental animal is put into the original position of any training chest at random, is started after experimental animal touch trigger device
One selection task, it is desirable to which two random one of arms of selection provide signal in corresponding training chest, and it is signal A, another nothing to make it
Signal.When experimental animal selection is provided with the selection arm of signal A, correct prompt tone is selected to sound, experimental animal can be
Reward area obtains reward S；If experimental animal selection is not provided with the selection arm of signal A, miscue sound equipment rises, is given without reward
And record corresponding selection situation；After experimental animal makes a choice, no matter select which arm, signal disappear, if in its arrival
Animal performs other operations before reward area, then miscue sound equipment rises, and is given without reward, records corresponding operational circumstances.If
Animal smoothly reaches reward area after selection, then select situation to obtain corresponding reward according to it, and the selection task terminates, equipment
Enter into next mission ready time.Likewise, animal can only start next selection after touch trigger device appointing
Business, the signal facilities of next selection task are performed depending on the situation of upper selection task according to animal, should be followed and " be entangled
It is wrong " principle：If the selection arm of setting signal A is selected in a upper selection task for experimental animal and arrival reward area is encouraged
Encourage, then next selection task signal is arranged on random selection arm；If experimental animal selects in a upper selection task
The selection arm for being not provided with signal A is selected, then the signal of next selection task sets ibid a selection task, i.e. signal and sets
Relative position is identical.Every experimental animal selection task number of times is not more than 100 daily, prevents influence animal behavior of overtraining.
（2）Risk training is the original position that experimental animal is put into any one training box, after experimental animal touch trigger device
Start a selection task, it is desirable to which two random one of arms of selection provide signal in corresponding training chest, and it is signal B to make it, another
Individual no signal.When experimental animal selection is provided with the selection arm of signal B, correct prompt tone is selected to sound, experimental animal can
Reward L is obtained with reward area；If experimental animal selection is not provided with the selection arm of signal B, miscue sound equipment rises, without reward
Give and record corresponding selection situation；After experimental animal makes a choice, no matter select which arm, signal disappear, if at it
Animal performs other operations before reaching reward area, then miscue sound equipment rises, and is given without reward, the corresponding operation feelings of record
Condition.If animal smoothly reaches reward area after selection, situation is selected to obtain corresponding reward, the selection task knot according to it
Beam, equipment enters into next mission ready time.Likewise, animal can only start after touch trigger device it is next
Selection task, the signal facilities of next selection task should be according to the situation of the upper selection task of animal execution
It is fixed, it then follows " error correction " principle：If experimental animal selects the selection arm of setting signal B in a upper selection task and reaches reward
Area is rewarded, then next selection task signal is arranged on random selection arm；If experimental animal is in upper one selection
Selection is not provided with the selection arm of signal B in task, then the signal of next selection task sets an ibid selection task, that is, believe
Number set relative position it is identical.Every experimental animal task number of times upper limit is 100 times daily, prevents the influence animal that overtrains
Behavior.
Step 6, memory consolidation training：Experimental animal is put into the original position of any one Experiment Training room, by animal
Autonomous to start training mission, task is divided into two parts, and Part I is trained with the secure item of step 5, the same step of the second part
Five risk training, it is random that two parts perform sequencing.When experimental animal can keep selection just in two kinds of tasks
True rate stabilization can then carry out the experiment of next step more than 80%.
Step 7, risk choice training：The training is divided into three parts, respectively secure item detection, risk detection, behavior
Choice.Preceding two parts detection method is trained with the secure item of step 5, risk training method, and the execution of preceding two parts detection is suitable
Sequence is random, only carries out the 3rd when experimental animal selects accuracy to reach more than 90% and stabilization in the detection of preceding two parts
Divide risk choice training.Each training chest random selection arm setting signal A, another selection arm setting signal B, work as experiment
After animal have selected certain arm, all blackouts, animal can obtain the reward that signal matches on the selection arm in reward area.
Using monitoring and record system monitoring and behavioural characteristic of the recording laboratory animal in the training stage, including selection situation, prize
Encourage situation, operating time, feedback etc..
In preference amendment training in above-mentioned steps four, if having the experimental animal of serious preference, that is, increasing above-mentioned
Supplemental training in the case of or when cannot correct the preference to side, then using intensive training mode, i.e., only in experiment
Certain reward could be obtained during the side that animal selection is seldom selected in reward area.Make again after the selection certain number of times of intensive training
Trained with preference amendment training method.
Present invention has the advantages that, the rat risk of above-mentioned design chooses the training method of behavior, training method and step
Design is more reasonable, more scientific, can significantly reduce the training time, and training for promotion efficiency, training has higher success rate, and can obtain true
Reliable experimental data, contributes to researcher to be efficiently monitored to animal behavior.
Specific embodiment
With reference to embodiment, the present invention is described further.
Rat risk chooses the training method of behavior, it is characterised in that as follows the step of its training method：
Step one, trainer prepare：Trainer will at least include training box, animal triggering device, selection arm, sound feedback
System, reward area, monitoring and record system；（1）Multiple training chests are set in the training box of setting；（2）The training chest of training box
Middle setting animal triggering device, experimental animal is required for touch trigger device before starting each training mission, it is ensured that experiment is in reality
Carried out on the premise of testing animal operant conditioned reflex；（3）Two profiles, structure equal indifferences are set in the training chest of training box
Selection arm, select arm species do not limit, the passage and depressible action bars passed through for experimental animal.Each selection arm
Structure on or surrounding space certain limit in independent mux --out signal exhibits area is set, be easy to experimental animal set up signal with it is corresponding
Contact between selection arm.Signal can be diversified forms, but need to ensure the signal influence such as nonirritant on experimental animal.At this
Signal is divided into two kinds in training mission, using the differentiation of figure or the differentiation of color or the differentiation of bright light number as reality
Test the two species diversity signals that animal can differentiate；（4）Sound feedback system, the selection feelings according to experimental animal are set in training box
Condition gives the prompting of certain sound feedback alternatively situation, such as the positive tone for raising up is set into correct selection prompting
Sound, the passive tone of decline is set to wrong choice prompt tone；（5）Reward area is set in the training chest of training box, is only tested
Animal reaches reward area and can obtain quantitative reward.Bonuses could be arranged to peanut, water, fruit juice etc. according to experimental program
The food of experimental animal enthusiasm can be transferred.According to task, reward is divided into two kinds, a kind of for reward probability is 100% to quantify
Small reward, is named as reward S, and the amount of reward S is depending on experimental design.It is quantitative big less than 100% that another kind is reward probability
Reward, is named as reward L, and the reward probability and reward amount for rewarding L can be depending on experimental designs；（6）Prison is set in training box
Control and record system, it is ensured that experimental animal state can in real time be monitored and record, record key element include the operating time of animal, choosing
Select situation, reward situation, feedback etc..
Step 2, experimental animal prepare：Experimental animal trains first 1 week by handle treatment, experimental animal is familiar with instruction
Practice personnel, exclude animal influences training effect because producing the moods such as fear, anxiety on experimenter；Sound is carried out after Handle
Amount strength test, the wave volume should ensure that experimental animal will not produce the exception row such as fear in the case of can perceiving sound
For record source of sound is away from the parameter such as experimental animal distance and decibels size, correct prompt tone and mistake in implementing for experiment
The parameter that prompt tone is set；Testing first three day need to carry out control water or control food preparation according to experimental design, keep the weight of animals not low
The 85% of normal type before food is controlled, it is ensured that make animal produce the dynamic of operation task in the case where animal bodies situation is not damaged
Machine.During the training period, after training makes the free diet of experimental animal to no longer diet after terminating every time, continue to control water or control food.Respectively
Can start formal training after the completion of item preparation.
Step 3, scene adaptation training：Experimental animal is put into the training chest original position in training box, makes it freely visit
Rope, once animal touch trigger device, then start a training mission, scene laundering period purpose is experimental animal is familiar with instruction
Practice place and its surrounding enviroment, understand training box structure, the award mode of familiarization training case and feature etc., therefore need not in training chest
Any signal is set, as long as experimental animal selects any selection arm after triggering device is started and reaches reward region, is sounded
Correct prompt tone simultaneously gives certain reward.If experimental animal repeats, multi-pass operation or faulty operation, mistake of sounding is carried
Show sound, given without reward.Each training mission is both needed to the autonomous touch trigger device of experimental animal to start.The behaviour of experimental animal
Make situation and reward situation is both needed to record, be easy to subsequently carry out statistical analysis to behavior task operating situation.Experiment number foundation
Reward is set depending on the situation of size and experimental animal, prevents influence animal behavior of overtraining.When experimental animal operation just
True rate reach more than 80% and it is continual and steady after think that experimental animal has realized training objective, turn to the preference profile to animal
Detected and corrected.
Step 4, preference detection and amendment training：Statistical experiment animal selects two to select arm in training box respectively
Number of times, compares whether they have notable difference, if not existing difference, then it is assumed that experimental animal selects a certain item in the absence of preference
Situation, without being modified training；If having differences, then it is assumed that experimental animal has the situation that preference selects a certain item.In order to
Prevent the study and selection of this preference influence animal in training below and experiment of experimental animal, the experiment for having preference from moving
Thing need to carry out preference amendment training.
It is every in training box using preference amendment training method for the rat for having preference in preference amendment training
Signal is not provided with individual selection arm, experimental animal unrestricted choice could be obtained when only successively selecting variant twice in reward area
Obtain and necessarily reward.For this regular experimental animal without law society, then increase supplemental training, i.e., selected by artificial side of blocking
The mode of arm helps the rat to carry out the training by selecting arm, and per supplemental training, 5 times the detection of freedom of entry selection mode is real again afterwards
The selection situation of animal is tested, for two selections time of selection arm in the case of experimental animal association alternate selection or unrestricted choice
When number is without significant difference, it is believed that experimental animal is without preference.
In preference amendment training, if having the experimental animal of serious preference, i.e., in the case where above-mentioned supplemental training is increased also
Be when cannot correct the preference to side, then using intensive training mode, i.e., for only seldom being selected in experimental animal selection
Certain reward could be obtained during side in reward area.Preference amendment training method instruction is reused after the selection certain number of times of intensive training
Practice.
Step 5, assignment training：Assignment training is divided into secure item training and risk training, two priorities of tasking learning
Order is random, and the study of the next item down task could be turned in the case of must ensureing a task association.Signal kinds and reward kind
Class need to remain matching.The selection situation of daily experimental animal is counted, when experimental animal selection is provided with the choosing of corresponding signal
The frequency for selecting arm reaches more than 80% in continuous 4 days, that is, select accuracy continuous 4 days up to more than 80%, then it is assumed that it has learned this
Task, reaches training objective.（1）Secure item training is that experimental animal is put into training box original position, if training box sets many
Individual training chest, then experimental animal is put into the original position of any training chest at random, is started after experimental animal touch trigger device
One selection task, it is desirable to which two random one of arms of selection provide signal in corresponding training chest, and it is signal A, another nothing to make it
Signal.When experimental animal selection is provided with the selection arm of signal A, correct prompt tone is selected to sound, experimental animal can be
Reward area obtains reward S；If experimental animal selection is not provided with the selection arm of signal A, miscue sound equipment rises, is given without reward
And record corresponding selection situation；After experimental animal makes a choice, no matter select which arm, signal disappear, if in its arrival
Animal performs other operations before reward area, then miscue sound equipment rises, and is given without reward, records corresponding operational circumstances.If
Animal smoothly reaches reward area after selection, then select situation to obtain corresponding reward according to it, and the selection task terminates, equipment
Enter into next mission ready time.Likewise, animal can only start next selection after touch trigger device appointing
Business, the signal facilities of next selection task are performed depending on the situation of upper selection task according to animal, should be followed and " be entangled
It is wrong " principle：If the selection arm of setting signal A is selected in a upper selection task for experimental animal and arrival reward area is encouraged
Encourage, then next selection task signal is arranged on random selection arm；If experimental animal selects in a upper selection task
The selection arm for being not provided with signal A is selected, then the signal of next selection task sets ibid a selection task, i.e. signal and sets
Relative position is identical.Every experimental animal selection task number of times is not more than 100 daily, prevents influence animal behavior of overtraining.
（2）Risk training is the original position that experimental animal is put into any one training box, after experimental animal touch trigger device
Start a selection task, it is desirable to which two random one of arms of selection provide signal in corresponding training chest, and it is signal B to make it, another
Individual no signal.When experimental animal selection is provided with the selection arm of signal B, correct prompt tone is selected to sound, experimental animal can
Reward L is obtained with reward area；If experimental animal selection is not provided with the selection arm of signal B, miscue sound equipment rises, without reward
Give and record corresponding selection situation；After experimental animal makes a choice, no matter select which arm, signal disappear, if at it
Animal performs other operations before reaching reward area, then miscue sound equipment rises, and is given without reward, the corresponding operation feelings of record
Condition.If animal smoothly reaches reward area after selection, situation is selected to obtain corresponding reward, the selection task knot according to it
Beam, equipment enters into next mission ready time.Likewise, animal can only start after touch trigger device it is next
Selection task, the signal facilities of next selection task should be according to the situation of the upper selection task of animal execution
It is fixed, it then follows " error correction " principle：If experimental animal selects the selection arm of setting signal B in a upper selection task and reaches reward
Area is rewarded, then next selection task signal is arranged on random selection arm；If experimental animal is in upper one selection
Selection is not provided with the selection arm of signal B in task, then the signal of next selection task sets an ibid selection task, that is, believe
Number set relative position it is identical.Every experimental animal task number of times upper limit is 100 times daily, prevents the influence animal that overtrains
Behavior.
Step 6, memory consolidation training：Experimental animal is put into the original position of any one Experiment Training room, by animal
Autonomous to start training mission, task is divided into two parts, and Part I is trained with the secure item of step 5, the same step of the second part
Five risk training, it is random that two parts perform sequencing.When experimental animal can keep selection just in two kinds of tasks
True rate stabilization can then carry out the experiment of next step more than 80%.
Step 7, risk choice training：The training is divided into three parts, respectively secure item detection, risk detection, behavior
Choice.Preceding two parts detection method is trained with the secure item of step 5, risk training method, and the execution of preceding two parts detection is suitable
Sequence is random, only carries out the 3rd when experimental animal selects accuracy to reach more than 90% and stabilization in the detection of preceding two parts
Divide risk choice training.Each training chest random selection arm setting signal A, another selection arm setting signal B, work as experiment
After animal have selected certain arm, all blackouts, animal can obtain the reward that signal matches on the selection arm in reward area.
Using monitoring and record system monitoring and behavioural characteristic of the recording laboratory animal in the training stage, including selection situation, prize
Encourage situation, operating time, feedback etc..
It should be noted that the invention is not restricted to above example, to those of ordinary skill in the art, not
On the premise of departing from inventive concept of the invention and principle, some improvements and modifications can also be made, these improvements and modifications
It is considered as protection scope of the present invention.
Claims (3)
1. rat risk chooses the training method of behavior, it is characterised in that as follows the step of its training method：
Step one, trainer prepare：Trainer will at least include training box, animal triggering device, selection arm, sound feedback
System, reward area, monitoring and record system；（1）Multiple training chest can be set in the training box of setting；（2）Training box it is each
Animal triggering device is set in individual training chest, and experimental animal is required for touch trigger device before starting each training mission, it is ensured that
Experiment is carried out on the premise of experimental animal operant conditioned reflex；（3）Two profiles, structures are set in the training chest of training box
The selection arm of equal indifference, selects the species of arm not limit, the passage and depressible action bars passed through for experimental animal；Often
Independent mux --out signal exhibits area is set on the structure of individual selection arm or in surrounding space certain limit, is easy to experimental animal to set up letter
Number contacting between corresponding selection arm；Signal can be diversified forms, but need to ensure that signal is nonirritant to experimental animal
Influence；Signal is divided into two kinds in the training mission, using the differentiation of figure or the differentiation of color or bright light number
Distinguishing two kinds can differentiated as experimental animal has difference signal；（4）Sound feedback system is set in training box, according to reality
The selection situation for testing animal gives the prompting of certain sound feedback alternatively situation, such as set the positive tone for raising up
It is correct selection prompt tone, the passive tone of decline is set to wrong choice prompt tone；（5）Prize is set in the training chest of training box
Area is encouraged, only experimental animal reaches reward area and can obtain quantitative reward；Bonuses could be arranged to according to experimental program
Peanut, water or fruit juice；According to task, reward is divided into two kinds, and a kind of is the quantitative small reward that reward probability is 100%, is named as prize
S is encouraged, the amount of reward S is depending on experimental design；Quantitative big reward of the another kind for reward probability less than 100%, is named as reward
L, the reward probability and reward amount for rewarding L can be depending on experimental designs；（6）Monitoring and record system are set in training box, are protected
Confirmation is tested animal state and can in real time be monitored and record, and record key element includes the operating time of animal, selection situation, reward situation
And feedback；
Step 2, experimental animal prepare：Experimental animal trains first 1 week by handle treatment, makes experimental animal familiarization training people
Member, exclude animal influences training effect because producing the moods such as fear, anxiety on experimenter；Volume is carried out after Handle strong
Degree test, it is adaptable to which the wave volume in experiment should ensure that experimental animal will not produce fear in the case of can perceiving sound
Etc. abnormal behaviour, record source of sound is away from experimental animal distance and the parameter of decibels size, the correct prompting in implementing for experiment
The parameter that sound and miscue sound are set；Testing first three day need to carry out control water or control food preparation according to experimental design, keep animal
Body weight is not less than the 85% of the preceding normal type of control food, it is ensured that animal is produced operation in the case where animal bodies situation is not damaged
The motivation of task；During the training period, every time training terminate after make the free diet of experimental animal to no longer diet after, continue control water or
Control food；Formal training can be started after the completion of every preparation；
Step 3, scene adaptation training：Experimental animal is put into the training chest original position in training box, makes it freely explore,
Once animal touch trigger device, then start a training mission, scene laundering period purpose is to make experimental animal familiarization training
Place and its surrounding enviroment, understanding training box structure, the award mode and feature of familiarization training case, therefore without setting in training chest
Any signal, as long as experimental animal selects any selection arm after triggering device is started and reaches reward region, sounds correct
Prompt tone simultaneously gives certain reward；If experimental animal repeats, multi-pass operation or faulty operation, miscue of sounding
Sound, gives without reward；Each training mission is both needed to the autonomous touch trigger device of experimental animal to start；The operation of experimental animal
Situation and reward situation are both needed to record, are easy to subsequently carry out statistical analysis to behavior task operating situation；Experiment number is according to prize
Encourage depending on the situation that size and experimental animal are set, prevent influence animal behavior of overtraining；When the operation of experimental animal is correct
Rate reach more than 80% and it is continual and steady after think that experimental animal has realized training objective, turn to and the preference profile of animal entered
Row detection is simultaneously corrected；
Step 4, preference detection and amendment training：Statistical experiment animal selects two number of times of selection arm in training box respectively,
Compare whether they have notable difference, if not existing difference, then it is assumed that experimental animal selects the situation of a certain item in the absence of preference,
Training need not be modified；If having differences, then it is assumed that experimental animal has the situation that preference selects a certain item；In order to prevent reality
Test this preference of animal influences the study and selection of animal in training below and experiment, and the experimental animal for having preference need to enter
The amendment of row preference is trained；
It is each choosing in training box using preference amendment training method for the rat for having preference in preference amendment training
Select and signal is not provided with arm, experimental animal unrestricted choice could obtain one when only successively selecting variant twice in reward area
Fixed reward；For this regular experimental animal without law society, then increase supplemental training, i.e., select arm by artificially blocking side
Mode helps rat to be trained, the selection feelings of freedom of entry selection mode test experience animal again afterwards per supplemental training 4~6 times
Condition, for two selection number of times of selection arm without significant difference in the case of experimental animal association alternate selection or unrestricted choice
When, it is believed that experimental animal is without preference；
Step 5, assignment training：Assignment training is divided into secure item training and risk training, two sequencings of tasking learning
At random, the study of the next item down task could be turned in the case of a task association must being ensured；Signal kinds and reward species are needed
Remain matching；The selection situation of daily experimental animal is counted, when experimental animal selection is provided with the selection arm of corresponding signal
Frequency reach more than 80% within continuous 4 days, that is, select continuous 4 days up to more than 80% of accuracy, then it is assumed that it has learned this task,
Reach training objective；（1）Secure item training is that experimental animal is put into training box original position, if training box sets multiple training
Room, then experimental animal is put into the original position of any training chest at random, and a choosing is started after experimental animal touch trigger device
Select task, it is desirable to which two random one of arms of selection provide signal in corresponding training chest, and it is signal A, another no signal to make it；When
When experimental animal selection is provided with the selection arm of signal A, correct prompt tone is selected to sound, experimental animal can be obtained in reward area
S must be rewarded；If experimental animal selection is not provided with the selection arm of signal A, miscue sound equipment is risen, and phase is given and recorded without reward
The selection situation answered；After experimental animal makes a choice, no matter select which arm, signal disappear, if before its arrival reward area
Animal performs other operations, then miscue sound equipment rises, and is given without reward, records corresponding operational circumstances；If animal is in choosing
Reward area is smoothly reached after selecting, then selects situation to obtain corresponding reward according to it, the selection task terminates, and equipment is entered into down
Individual mission ready time；Likewise, animal can only start next selection task after touch trigger device, it is next
The signal facilities of selection task are performed depending on the situation of upper selection task according to animal, should follow " error correction " principle：
If the selection arm of setting signal A is selected in a upper selection task for experimental animal and arrival reward area is rewarded, next
Individual selection task signal is arranged on random selection arm；If experimental animal selects to be not provided with letter in a upper selection task
The selection arm of number A, then the signal of next selection task ibid a selection task, i.e., the relative position phase that signal is set are set
Together；Every experimental animal selection task number of times is not more than 100 daily, prevents influence animal behavior of overtraining；（2）Risk instruction
White silk is the original position that experimental animal is put into any one training box, and a selection is started after experimental animal touch trigger device
Task, it is desirable to which two random one of arms of selection provide signal in corresponding training chest, and it is signal B, another no signal to make it；Work as reality
When testing animal selection and being provided with the selection arm of signal B, correct prompt tone is selected to sound, experimental animal can obtain in reward area
Reward L；If experimental animal selection is not provided with the selection arm of signal B, miscue sound equipment rises, and gives and record corresponding without reward
Selection situation；After experimental animal makes a choice, no matter select which arm, signal disappear, if in its arrival reward Qu Qiandong
Thing performs other operations, then miscue sound equipment rises, and is given without reward, records corresponding operational circumstances；If animal is in selection
Reward area smoothly being reached afterwards, then selecting situation to obtain corresponding reward according to it, the selection task terminates, and equipment enters into next
Mission ready time；Likewise, animal can only start next selection task, next choosing after touch trigger device
Select task signal facilities should according to animal perform it is upper one selection task situation depending on, it then follows " error correction " principle：
If the selection arm of setting signal B is selected in a upper selection task for experimental animal and arrival reward area is rewarded, next
Individual selection task signal is arranged on random selection arm；If experimental animal selects to be not provided with letter in a upper selection task
The selection arm of number B, then the signal of next selection task ibid a selection task, i.e., the relative position phase that signal is set are set
Together；Every experimental animal task number of times upper limit is 100 times daily, prevents influence animal behavior of overtraining；
Step 6, memory consolidation training：Experimental animal is put into the original position of any one Experiment Training room, it is autonomous by animal
Start training mission, task is divided into two parts, and Part I is trained with the secure item of step 5, and the second part is with step 5
Risk training, it is random that two parts perform sequencing；When experimental animal can keep selecting accuracy in two kinds of tasks
Stabilization can then carry out the experiment of next step more than 80%；
Step 7, risk choice training：The training is divided into three parts, and respectively secure item detection, risk detection, behavior is picked out
Select；Preceding two parts detection method is trained with the secure item of step 5, risk training method, the execution sequence of preceding two parts detection
At random, only Part III is carried out when experimental animal selects accuracy to reach more than 90% and stabilization in the detection of preceding two parts
Risk choice training；Each training chest random selection arm setting signal A, another selection arm setting signal B, when experiment is dynamic
After thing have selected certain arm, all blackouts, animal can obtain the reward that signal matches on the selection arm in reward area；Profit
Monitored and behavioural characteristic of the recording laboratory animal in the training stage with monitoring and record system, including selection situation, reward
Situation, operating time and feedback.
2. the behavioral pharmacology research device that rat risk according to claim 1 is chosen, it is characterised in that：Step 4
In preference amendment training, if having the experimental animal of serious preference, i.e., still cannot change in the case where supplemental training is increased
When just to the preference of side, then using intensive training mode, i.e., only could at the side that experimental animal selection is seldom selected
Certain reward is obtained in reward area；The training of preference amendment training method is reused after the selection certain number of times of intensive training.
3. the behavioral pharmacology research device that rat risk according to claim 1 is chosen, it is characterised in that：In step 5
" error correction " system, after experimental animal is cooked the selection for making mistake, the method to set up and phase of signal when next subtask is set
It is identical with last task holding to position.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710030684.3A CN106889022B (en) | 2017-01-17 | 2017-01-17 | Training method for rat risk decision behavior |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710030684.3A CN106889022B (en) | 2017-01-17 | 2017-01-17 | Training method for rat risk decision behavior |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106889022A true CN106889022A (en) | 2017-06-27 |
| CN106889022B CN106889022B (en) | 2019-12-20 |
Family
ID=59198975
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710030684.3A Active CN106889022B (en) | 2017-01-17 | 2017-01-17 | Training method for rat risk decision behavior |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106889022B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077183A (en) * | 2018-01-29 | 2018-05-29 | 王存金 | A kind of animal risk taking behavior test device and its test method |
| CN112535115A (en) * | 2020-12-10 | 2021-03-23 | 南昌大学 | Behavior training device and method for researching rat risk choice |
| CN113016654A (en) * | 2021-03-25 | 2021-06-25 | 复旦大学 | Visual information-oriented spatial memory behavior training method |
| CN113392975A (en) * | 2021-07-07 | 2021-09-14 | 温州医科大学 | Construction method of delay reward benefit and fraud trade-off decision-making behavioural model |
| CN113796335A (en) * | 2021-08-26 | 2021-12-17 | 五邑大学 | Control method, device and equipment of mouse behavior training system and storage medium |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817326A (en) * | 2006-02-21 | 2006-08-16 | 沈阳医学院 | Mouse space memory retention model and intelligent action inspecting system |
| CN103125406A (en) * | 2013-03-19 | 2013-06-05 | 郑州大学 | Visual cognitive behavioral learning automatic training system of big and small mice |
| CN104663490A (en) * | 2015-03-03 | 2015-06-03 | 广西南宁灵康赛诺科生物科技有限公司 | Automatic device for evaluating memory function of primate experimental animal |
| CN106508714A (en) * | 2016-10-25 | 2017-03-22 | 郑州大学 | Animal object target recognizing learning training system |
-
2017
- 2017-01-17 CN CN201710030684.3A patent/CN106889022B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1817326A (en) * | 2006-02-21 | 2006-08-16 | 沈阳医学院 | Mouse space memory retention model and intelligent action inspecting system |
| CN103125406A (en) * | 2013-03-19 | 2013-06-05 | 郑州大学 | Visual cognitive behavioral learning automatic training system of big and small mice |
| CN104663490A (en) * | 2015-03-03 | 2015-06-03 | 广西南宁灵康赛诺科生物科技有限公司 | Automatic device for evaluating memory function of primate experimental animal |
| CN106508714A (en) * | 2016-10-25 | 2017-03-22 | 郑州大学 | Animal object target recognizing learning training system |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077183A (en) * | 2018-01-29 | 2018-05-29 | 王存金 | A kind of animal risk taking behavior test device and its test method |
| CN112535115A (en) * | 2020-12-10 | 2021-03-23 | 南昌大学 | Behavior training device and method for researching rat risk choice |
| CN113016654A (en) * | 2021-03-25 | 2021-06-25 | 复旦大学 | Visual information-oriented spatial memory behavior training method |
| CN113016654B (en) * | 2021-03-25 | 2021-12-10 | 复旦大学 | Visual information-oriented spatial memory behavior training method |
| CN113392975A (en) * | 2021-07-07 | 2021-09-14 | 温州医科大学 | Construction method of delay reward benefit and fraud trade-off decision-making behavioural model |
| CN113392975B (en) * | 2021-07-07 | 2024-02-06 | 温州医科大学 | Construction method of delayed rewarding, cheating and balancing decision-making behavioural model |
| CN113796335A (en) * | 2021-08-26 | 2021-12-17 | 五邑大学 | Control method, device and equipment of mouse behavior training system and storage medium |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106889022B (en) | 2019-12-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106889022A (en) | Rat risk chooses the training method of behavior | |
| Bosman et al. | Functions of gamma‐band synchronization in cognition: From single circuits to functional diversity across cortical and subcortical systems | |
| Miendlarzewska et al. | Influence of reward motivation on human declarative memory | |
| Healey et al. | A four-component model of age-related memory change. | |
| Lee et al. | Mechanisms of reinforcement learning and decision making in the primate dorsolateral prefrontal cortex | |
| Ashby et al. | 4 COVIS | |
| Nakazawa et al. | Hippocampal CA3 NMDA receptors are crucial for memory acquisition of one-time experience | |
| Bose et al. | Effect of the environment on the dendritic morphology of the rat auditory cortex | |
| Reynolds et al. | Sensory and motor characterization in the postnatal valproate rat model of autism | |
| Hardcastle et al. | What do brain data really show? | |
| CN100471466C (en) | Mouse space memory retention model and intelligent action inspecting system | |
| Carlson et al. | Dynamically timed stimulation of corticolimbic circuitry activates a stress-compensatory pathway | |
| Bailey et al. | Lesions of specific and nonspecific thalamic nuclei affect prefrontal cortex-dependent aspects of spatial working memory. | |
| CN1817325A (en) | Mouse hearing and vision memory model and intelligent action inspecting system | |
| Schmajuk | Mechanisms in classical conditioning: A computational approach | |
| CN110866537B (en) | Brain wave-based emotion recognition method for game evaluation | |
| CN100477970C (en) | Mouse path exercising model and intelligent action inspecting system | |
| Lorsch et al. | Computational analysis of multidimensional behavioral alterations after chronic social defeat stress | |
| Quinn et al. | Learning‐dependent dynamics of beta‐frequency oscillations in the basal forebrain of rats | |
| Mannella et al. | The interplay of pavlovian and instrumental processes in devaluation experiments: a computational embodied neuroscience model tested with a simulated rat | |
| Brodbeck et al. | Timing behaviour of black-capped chickadees (Parus atricapillus) | |
| Hahn et al. | Executive control of sequence behavior in pigeons involves two distinct brain regions | |
| Prescott et al. | The robot basal ganglia: action selection by an embedded model of the basal ganglia | |
| Callu et al. | Cognitive processing impairments in a supra-second temporal discrimination task in rats with cerebellar lesion | |
| Delamater et al. | Learning what to expect and when to expect it involves dissociable neural systems |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |