CN108072586A - A kind of microbial degradation respirometer - Google Patents
A kind of microbial degradation respirometer Download PDFInfo
- Publication number
- CN108072586A CN108072586A CN201711465965.8A CN201711465965A CN108072586A CN 108072586 A CN108072586 A CN 108072586A CN 201711465965 A CN201711465965 A CN 201711465965A CN 108072586 A CN108072586 A CN 108072586A
- Authority
- CN
- China
- Prior art keywords
- pipe
- vertical pivot
- reaction
- gas
- reaction bulb
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 30
- 230000000813 microbial effect Effects 0.000 title claims abstract description 28
- 230000015556 catabolic process Effects 0.000 title claims abstract description 27
- 238000006243 chemical reaction Methods 0.000 claims abstract description 100
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- 230000000087 stabilizing effect Effects 0.000 claims abstract description 7
- 239000007789 gas Substances 0.000 claims description 35
- 238000010521 absorption reaction Methods 0.000 claims description 28
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 24
- 239000001301 oxygen Substances 0.000 claims description 24
- 229910052760 oxygen Inorganic materials 0.000 claims description 24
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 23
- 238000003756 stirring Methods 0.000 claims description 17
- 239000012670 alkaline solution Substances 0.000 claims description 14
- 238000003760 magnetic stirring Methods 0.000 claims description 14
- 239000001569 carbon dioxide Substances 0.000 claims description 12
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 12
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 10
- 238000010907 mechanical stirring Methods 0.000 claims description 10
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims description 9
- 238000012360 testing method Methods 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 6
- 239000003643 water by type Substances 0.000 claims 1
- 239000000243 solution Substances 0.000 description 11
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 8
- 238000005859 coupling reaction Methods 0.000 description 7
- 239000003792 electrolyte Substances 0.000 description 6
- 238000010586 diagram Methods 0.000 description 4
- 230000005611 electricity Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 239000012530 fluid Substances 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 239000000565 sealant Substances 0.000 description 2
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000002144 chemical decomposition reaction Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 238000005868 electrolysis reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003344 environmental pollutant Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000008246 gaseous mixture Substances 0.000 description 1
- 239000011261 inert gas Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 230000001706 oxygenating effect Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 238000005375 photometry Methods 0.000 description 1
- 231100000719 pollutant Toxicity 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N7/00—Analysing materials by measuring the pressure or volume of a gas or vapour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N7/00—Analysing materials by measuring the pressure or volume of a gas or vapour
- G01N7/14—Analysing materials by measuring the pressure or volume of a gas or vapour by allowing the material to emit a gas or vapour, e.g. water vapour, and measuring a pressure or volume difference
- G01N7/18—Analysing materials by measuring the pressure or volume of a gas or vapour by allowing the material to emit a gas or vapour, e.g. water vapour, and measuring a pressure or volume difference by allowing the material to react
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of microbial degradation respirometers, are related to field of environment protection, the reaction bulb, metering pool including thermostat water bath, with bottle cap and the conduit for connecting the reaction bulb and metering pool；The reaction bulb is located in the thermostat water bath, and the bottle cap is equipped with the gas-guide tube with being connected inside the reaction bulb, and agitating device is equipped in the reaction bulb；It is less than the second vertical pivot pipe of the first vertical pivot pipe and positioned at the first vertical pivot pipe lower section and inclined inclined pipe equipped with the first vertical pivot pipe, length in the metering pool, the lower port of the first vertical pivot pipe and the second vertical pivot pipe respectively connects the end of an inclined pipe, and the end that inclined pipe is connected with the second vertical pivot pipe is equipped with pressure stabilizing chamber.The present invention can not only carry out aerobic reaction, can also carry out anaerobic reaction, applied widely.
Description
Technical field
The present invention relates to field of environment protection, and in particular to a kind of microbial degradation respirometer.
Background technology
With the continuous propulsion of human society industrial civilization, problem of environmental pollution increasingly aggravates, and seriously constrains the mankind's
Sustainable development, therefore, realize effective degradation of pollutant has significant meaning to improving environment.Since chemical degradation has
Machine object is also easy to produce many harmful or noxious materials, therefore microbial degradation organic matter is by progressively promotion and application.Microbial degradation
Refer to organic matter is changed into simple inorganic matter by microorganism, microbial degradation is generally divided into aerobic reaction and anaerobic reaction two
Class, and the amount for generating gas during accurate measurement microbial degradation or consuming oxygen has microorganisms degradation reaction
Important meaning.
It is electrolytic respirometer that for microbial degradation repercussion study, application is more at present, and operation principle is microorganism
Oxygen is absorbed, release carbon dioxide and is absorbed by alkaline solution (such as sodium hydroxide solution), air pressure in reative cell is caused to reduce,
And perceived by indoor condenser type micro-pressure sensor is reacted, feed back to the Faradaic current that oxygenating circuit the output phase is answered, electrolysis electricity
Stream generates oxygen by electrolyte (such as copper-bath) and fills into reative cell, reaches dynamic equilibrium, then by measuring phase
The electric current answered can calculate the amount of oxygen of consumption in reative cell, and then grasp the extent of reaction during microbial degradation.Electricity
The operation principle of solution formula respirometer limits it and is only capable of doing aerobic reaction research, and other dresses are must be equipped with if anaerobic reaction is carried out
It puts.It installs complicated, expensive and needs to regularly replace the problems such as electrolyte in addition, electrolytic respirometer exists, give
The research of microbial degradation reaction brings inconvenience.
The content of the invention
For defect in the prior art, it is an object of the invention to provide a kind of microbial degradation respirometer, no
Aerobic reaction can be only carried out, can also carry out anaerobic reaction, it is applied widely.
To achieve the above objectives, the present invention adopts the technical scheme that, including：
Thermostat water bath；
Reaction bulb with bottle cap, the reaction bulb are located in the thermostat water bath, the bottle cap be equipped with it is described anti-
The gas-guide tube of connection inside bottle is answered, and agitating device is equipped in the reaction bulb；
Metering pool, in the metering pool equipped with the first vertical pivot pipe, length be less than the first vertical pivot pipe the second vertical pivot pipe and
Below the first vertical pivot pipe and inclined inclined pipe, each connection one of lower port of the first vertical pivot pipe and the second vertical pivot pipe are oblique
The end taken over, and the end that inclined pipe is connected with the second vertical pivot pipe is equipped with pressure stabilizing chamber, the metering pool top is equipped with and institute
The air outlet pipe of connection and the air inlet pipe with being connected inside the second vertical pivot pipe inside the first vertical pivot pipe are stated, described first is perpendicular
Equipped with liquid in central siphon, and the lower end of the first vertical pivot pipe is equipped with photo counter；
For connecting the conduit of the reaction bulb and metering pool, and when the conduit is equipped with for absorbing carbon dioxide
During absorption bottle, one end of the conduit connects the gas-guide tube of the reaction bulb, when the other end connects the air inlet pipe of the metering pool
Anaerobic reaction is carried out, when one end of the conduit connects the gas-guide tube of the reaction bulb, the other end connects the air outlet pipe of metering pool,
And the air inlet pipe of the metering pool carries out aerobic reaction when being passed through oxygen.
Based on the above technical solutions, the agitating device be magnetic stirring apparatus, the magnetic stirring apparatus bag
Include the magneton in the reaction bulb and the magnetic stirring apparatus positioned at the thermostat water bath outer bottom.
Based on the above technical solutions, the absorption bottle includes the U-shaped cup for being located at bottle mouth position in the reaction bulb, institute
It states gas-guide tube and stretches into the part of reaction bulb and be located in the U-shaped cup, the surrounding of the U-shaped cup offers multiple perforates, described U-shaped
Test tube is placed in cup, and the alkaline solution for absorbing carbon dioxide is mounted in the test tube.
Based on the above technical solutions, the agitating device be mechanical stirring device, the mechanical stirring device bag
The motor above the bottle cap and the stirring rod in the reaction bulb are included, straight tube is additionally provided on the bottle cap, it is described
The shaft of motor is arranged in the bottle cap, and seal pipe is arranged on the stirring rod, and the top of the stirring rod is located at straight tube
It is interior, connecting tube is equipped with below the shaft of the motor, the shaft of the motor is connected by connecting tube with the stirring rod.
Based on the above technical solutions, on conduit of the absorption bottle between reaction bulb and metering pool, the suction
It receives bottle and is equipped with air inlet and gas outlet, and the conduit at the absorption bottle in fracture shape and forms two fractures, wherein one
Fracture connects the air inlet of the absorption bottle, and another fracture connects the gas outlet of the absorption bottle, is mounted in the absorption bottle
For the alkaline solution of absorbing carbon dioxide.
Based on the above technical solutions, the alkaline solution be sodium hydroxide or potassium hydroxide, the four of the bottle cap
Week is equipped with sealing ring.
Based on the above technical solutions, oxygen cylinder is further included, one end of the conduit connects leading for the reaction bulb
Tracheae, the other end connects the air outlet pipe of metering pool, and the air inlet pipe of the metering pool connects oxygen cylinder to carry out aerobic reaction, institute
It states and gas row is equipped between the gas-guide tube of reaction bulb and oxygen cylinder.
Based on the above technical solutions, gas collection bag is further included, one end of the conduit connects the reaction bulb
Gas-guide tube, the other end connects the air inlet pipe of the metering pool, and the air outlet pipe of the metering pool connects the gas collection bag
When to carry out anaerobic reaction.
Based on the above technical solutions, the interior of metering pool is fixed in the end of the second vertical pivot pipe of the inclined pipe connection
On side wall, fix and seal between the inner top surface of the upper port and the metering pool of the first vertical pivot pipe and the second vertical pivot pipe.
Based on the above technical solutions, nut is arranged in the air inlet pipe and air outlet pipe of the metering pool, it is described
Air inlet pipe and air outlet pipe are fixed on by nut at the top of metering pool.
Compared with prior art, the advantage of the invention is that：Overall structure is simple, at low cost, can not only carry out aerobic
Reaction, can also carry out anaerobic reaction, applied widely, and pipeline removes and installs just when being converted between aerobic reaction and anaerobic reaction
Victory, conversion is convenient, and can automate real-time monitoring, and when work can be unattended, while respirometer is integrally without electrolyte, thus
Cumbersome electrolyte need not be carried out to hunt leak with more changing jobs.
Description of the drawings
Fig. 1 is the schematic diagram that the present invention carries out aerobic reaction using mechanical stirring device；
Fig. 2 is the schematic diagram that the present invention carries out aerobic reaction using magnetic stirring apparatus；
Fig. 3 is the schematic diagram that the present invention carries out anaerobic reaction using mechanical stirring device；
Fig. 4 is the schematic diagram that the present invention carries out anaerobic reaction using magnetic stirring apparatus.
In figure：1- thermostat water baths, 2- bottle caps, 3- reaction bulbs, 4- gas-guide tubes, 5- agitating devices, 6- metering pools, 7- first
Vertical pivot pipe, 8- the second vertical pivot pipes, 9- inclined pipes, 10- pressure stabilizing chambers, 11- air outlet pipes, 12- air inlet pipe, 13- liquids, 14- photometry numbers
Device, 15- conduits, 16- absorption bottles, 17- magnetons, 18- magnetic stirring apparatus, 19-U type cups, 20- test tubes, 21- motors, 22- stirrings
Bar, 23- seal pipes, 24- straight tubes, 25- shafts, 26- connecting tubes, 27- air inlets, 28- gas outlets, 29- gas collection bags, 30-
Nut, 31- oxygen cylinders, 32- gas row.
Specific embodiment
The present invention is described in further detail below in conjunction with attached drawing.
Shown in Figure 1, the present invention provides a kind of microbial degradation respirometer, can carry out aerobic reaction and also carry out anaerobism
Reaction specifically includes thermostat water bath 1, the reaction bulb 3 with bottle cap 2, metering pool 6 and for coupled reaction bottle 3 and metering pool 6
Conduit 15.
Reaction bulb 3 is located in thermostat water bath 1, to carry out temperature control experiment.Bottle cap 2 is equipped with to be connected with 3 inside of reaction bulb
Gas-guide tube 4, and reaction bulb 3 in be equipped with agitating device 5, agitating device 5 be magnetic stirring apparatus or mechanical stirring device.Bottle cap
2 are surrounded by sealing ring, when tighten bottle cap 2 to reaction bulb 3 bottleneck when sealing ring stress deform upon, squeezing bottleneck makes bottle
Sealing system is formed between lid 2 and bottleneck.Fluid sealant is scribbled between gas-guide tube 4 and bottle cap 2, is ensured close between gas-guide tube 4 and bottle cap 2
Envelope, 3 inside of reaction bulb form a sealing system.Inert gas can import by gas-guide tube 4 in reaction bulb 3 to ensure reaction bulb 3
Interior anaerobic environment, the gas that can be also generated microbial degradation by gas-guide tube 4 export reaction bulb 3, can also be dynamic by outside
Power such as peristaltic pump import reaction mass in reaction bulb 3 through gas-guide tube 4.
It is less than the second vertical pivot pipe 8 of the first vertical pivot pipe 7 and positioned at first equipped with the first vertical pivot pipe 7, length in metering pool 6
Below vertical pivot pipe 7 and inclined inclined pipe 9, the first vertical pivot pipe 7 respectively connect an inclined pipe 9 with the lower port of the second vertical pivot pipe 8
End, the end that inclined pipe 9 connects the second vertical pivot pipe 8 are fixed on the madial wall of metering pool 6, and the first vertical pivot pipe 7 and second is perpendicular
It fixes and seals between the upper port of central siphon 8 and the inner top surface of metering pool 6, and on the end that is connected with the second vertical pivot pipe 8 of inclined pipe 9
Equipped with pressure stabilizing chamber 10, specifically, 9 and second vertical pivot pipe of inclined pipe, 8 junction to the part between 6 inner wall of metering pool is pressure stabilizing chamber
10, pressure stabilizing chamber 10 is used to buffer the flow velocity of gas cataclysm in metering pool 6.6 top of metering pool be equipped with inside the first vertical pivot pipe 7
The air outlet pipe 11 of connection and the air inlet pipe 12 connected with 8 inside of the second vertical pivot pipe, the air inlet pipe 12 and air outlet pipe of metering pool 6
Nut 30, air inlet pipe 12 and air outlet pipe 11 are arranged on 11,6 top of metering pool is fixed on by nut 30.
Equipped with liquid 13 in first vertical pivot pipe 7, and the lower end of the first vertical pivot pipe 7 is equipped with photo counter 14, photo counter 14
Including light-emitting tube, light receiving tube, meter and data collecting card, liquid 13 is water, oil or other liquid, and gas is passing through the
Bubble is generated during liquid 13 in one vertical pivot pipe 7, photo counter 14 measures the bubble number of generation, to make generation bubble
Size it is identical in order to the later stage calculate gas volume, some accessories can be increased on the pipeline of gas, can ensure each gas
The size of bubble is identical under a certain range of gas flow rate.
When conduit 15 is equipped with the absorption bottle 16 for absorbing carbon dioxide, one end coupled reaction bottle 3 of conduit 15
Gas-guide tube 4, the other end carry out anaerobic reaction when connecting the air inlet pipe 12 of metering pool 6, when one end coupled reaction bottle 3 of conduit 15
Gas-guide tube 4, the other end connect the air outlet pipe 11 of metering pool 6, and when air inlet pipe 12 of metering pool 6 is passed through oxygen carry out it is aerobic anti-
It should.The material of conduit 15 is rubber.Specifically have by following instance to the application of microbial degradation respirometer of the present invention
Body explanation.
Shown in Figure 1, when mechanical stirring device is used to carry out aerobic reaction, for mechanical stirring device, machinery stirs
Mixing device includes the motor 21 for being located at 2 top of bottle cap and the stirring rod 22 in reaction bulb 3, and straight tube 24 is additionally provided on bottle cap 2,
Fluid sealant is scribbled between straight tube 24 and bottle cap 2, the shaft 25 of motor 21 is arranged in bottle cap 2, and seal pipe is arranged on stirring rod 22
23, the top of stirring rod 22 is located in straight tube 24, and 25 lower section of shaft of motor 21 is equipped with connecting tube 26, and the shaft 25 of motor 21 is logical
It crosses connecting tube 26 with stirring rod 22 to be connected, by motor 21 stirring rod 22 is driven to rotate to be carried out to the reactant in reaction bulb 3
Stirring.Motor 21 is by screw tightening on 21 pedestal of motor, and fixed between 21 pedestal of motor and bottle cap 2, motor 21 is equipped with electricity
Source line, power cord are connected with external power supply.
The gas-guide tube 4 of one end coupled reaction bottle 3 of conduit 15, the other end connect the air outlet pipe 11 of metering pool 6, and metering pool
6 air inlet pipe 12 connects oxygen cylinder 31, and the oxygen of oxygen cylinder 31 is through the second vertical pivot pipe 8, inclined pipe 9, the first vertical pivot pipe 7 and conduit
15 enter in reaction bulb 3, carry out aerobic reaction at this time, and it is aerobic reaction that photo counter 14, which calculates the gas flow measured, at this time
When the amount of oxygen that consumes.Further, gas is equipped between the gas-guide tube 4 of reaction bulb 3 and oxygen cylinder 31 and arranges 32, so as to pass through one
A oxygen cylinder 31 and a gas row 32 supply to multiple metering pools 6, and one end of gas row 32 can be opened wide, in order to being passed through metering pool
6 oxygen gas flow rate buffering.
Shown in Figure 2, when magnetic stirring apparatus is used to carry out aerobic reaction, for magnetic stirring apparatus, magnetic force stirs
Mixing device includes the magneton 17 being located in reaction bulb 3 and the magnetic stirring apparatus 18 positioned at 1 outer bottom of thermostat water bath, passes through magnetic
Power blender 18 controls the mixing speed of the magneton 17 in reaction bulb 3.
The gas-guide tube 4 of one end coupled reaction bottle 3 of conduit 15, the other end connect the air outlet pipe 11 of metering pool 6, and metering pool
6 air inlet pipe 12 connects oxygen cylinder 31, and the oxygen of oxygen cylinder 31 is through the second vertical pivot pipe 8, inclined pipe 9, the first vertical pivot pipe 7 and conduit
15 enter in reaction bulb 3, carry out aerobic reaction at this time, and it is aerobic reaction that photo counter 14, which calculates the gas flow measured, at this time
When the amount of oxygen that consumes.
It is shown in Figure 3, when mechanical stirring device is used to carry out anaerobic reaction, the structure of mechanical stirring device and anti-
Answer the arrangement on bottle 3 identical with example one, and the conduit 15 between reaction bulb 3 and metering pool 6 is equipped with absorption bottle 16, absorption bottle
16 are equipped with air inlet 27 and gas outlet 28, and the conduit 15 at absorption bottle 16 in fracture shape and forms two fractures, i.e., should
The conduit 15 at place disconnects and forms two fractures, wherein the air inlet 27 of fracture connection absorption bottle 16, another fracture connection is inhaled
The gas outlet 28 of bottle 16 is received, the alkaline solution for absorbing carbon dioxide is mounted in absorption bottle 16, alkaline solution is hydroxide
Sodium or potassium hydroxide.
The gas-guide tube 4 of one end coupled reaction bottle 3 of conduit 15, the other end connect the air inlet pipe 12 of metering pool 6, and metering pool
6 air outlet pipe 11 connects gas collection bag 29 or opens wide, and carries out anaerobic reaction at this time.When absorption bottle 16 does not fill alkaline solution,
It is the mixed gas yield such as methane, carbon dioxide that photo counter 14, which calculates the gas flow measured, at this time, when absorption bottle 16
It is interior be equipped with alkaline solution when, at this time photo counter 14 calculate measure mainly methane gas yield.
Shown in Figure 4, when magnetic stirring apparatus is used to carry out anaerobic reaction, magnetic stirring apparatus is on reaction bulb 3
Arrangement it is identical with example two, for absorption bottle 16, absorption bottle 16 include be located at reaction bulb 3 in bottle mouth position U-shaped cup 19, it is U-shaped
Cup 19 is plastic material and hangs on bottle mouth position, and the part that gas-guide tube 4 stretches into reaction bulb 3 is located in U-shaped cup 19, U-shaped cup 19
Surrounding offers multiple perforates for breathing freely, and test tube 20 is placed in U-shaped cup 19, and U-shaped cup 19 has certain deformability, and
The upper width of U-shaped cup 19 is more than the width of lower part, and test tube 20 is fixed in U-shaped cup 19, and is mounted with to inhale in test tube 20
Receive the alkaline solution of carbon dioxide.
The gas-guide tube 4 of one end coupled reaction bottle 3 of conduit 15, the other end connect the air inlet pipe 12 of metering pool 6, and metering pool
6 air outlet pipe 11 connects gas collection bag 29 or opens wide, and carries out anaerobic reaction at this time.When absorption bottle 16 does not fill alkaline solution,
It is the gaseous mixtures scale of construction such as methane, carbon dioxide that photo counter 14, which calculates the gas flow measured, at this time, when absorption bottle 16 is built-in
When having alkaline solution, photo counter 14 calculates the gas flow for the mainly methane measured at this time.
The microbial degradation respirometer of the present invention, overall structure is simple, at low cost, can not only carry out aerobic reaction,
It can carry out anaerobic reaction, it is applied widely, and pipeline removes and installs convenient, this hair when being converted between aerobic reaction and anaerobic reaction
Bright microbial degradation respirometer can automate real-time monitoring, and when work can be unattended, while whole without electrolyte, thus
It need not carry out cumbersome electrolyte leak detection and more change jobs, the research for greatly facilitating microbial degradation reaction carries out.
The present invention is not limited to the above-described embodiments, for those skilled in the art, is not departing from
On the premise of the principle of the invention, several improvements and modifications can also be made, these improvements and modifications are also considered as the protection of the present invention
Within the scope of.The content not being described in detail in this specification belongs to the prior art well known to professional and technical personnel in the field.
Claims (10)
1. a kind of microbial degradation respirometer, which is characterized in that including：
Thermostat water bath (1)；
Reaction bulb (3) with bottle cap (2), the reaction bulb (3) are located in the thermostat water bath (1), are set on the bottle cap (2)
There is the gas-guide tube (4) with being connected inside the reaction bulb (3), and agitating device (5) is equipped in the reaction bulb (3)；
Metering pool (6), the metering pool (6) is interior to erect equipped with second of the first vertical pivot pipe (7), length less than the first vertical pivot pipe (7)
Central siphon (8) and below the first vertical pivot pipe (7) and inclined inclined pipe (9), the first vertical pivot pipe (7) and the second vertical pivot
The end of each one inclined pipe of connection (9) of the lower port of (8) is managed, and is set on the end that is connected with the second vertical pivot pipe (8) of inclined pipe (9)
There is pressure stabilizing chamber (10), metering pool (6) top is equipped with the air outlet pipe (11) with being connected inside the first vertical pivot pipe (7), with
And the air inlet pipe (12) with being connected inside the second vertical pivot pipe (8), the first vertical pivot pipe (7) is interior equipped with liquid (13), and
The lower end of the first vertical pivot pipe (7) is equipped with photo counter (14)；
For connecting the conduit (15) of the reaction bulb (3) and metering pool (6), and when the conduit (15) is equipped with to absorb
During absorption bottle (16) of carbon dioxide, one end of the conduit (15) connects the gas-guide tube (4) of the reaction bulb (3), the other end
Anaerobic reaction is carried out during air inlet pipe (12) for connecting the metering pool (6), when one end of the conduit (15) connects the reaction
The gas-guide tube (4) of bottle (3), the air outlet pipe (11) of other end connection metering pool (6), and the air inlet pipe (12) of the metering pool (6)
Aerobic reaction is carried out when being passed through oxygen.
2. a kind of microbial degradation respirometer as described in claim 1, it is characterised in that：The agitating device (5) is magnetic force
Agitating device, the magnetic stirring apparatus include the magneton (17) being located in the reaction bulb (3) and positioned at the thermostatted waters
The magnetic stirring apparatus (18) of bath (1) outer bottom.
3. a kind of microbial degradation respirometer as claimed in claim 2, it is characterised in that：The absorption bottle (16) includes being located at
The U-shaped cup (19) of the interior bottle mouth position of the reaction bulb (3), the gas-guide tube (4) stretch into the part of reaction bulb (3) positioned at described U-shaped
In cup (19), the surrounding of the U-shaped cup (19) offers multiple perforates, and test tube (20), and institute are placed in the U-shaped cup (19)
State the alkaline solution being mounted in test tube (20) for absorbing carbon dioxide.
4. a kind of microbial degradation respirometer as described in claim 1, it is characterised in that：The agitating device (5) is machinery
Agitating device, the mechanical stirring device is including the motor (21) above the bottle cap (2) and positioned at the reaction bulb (3)
Interior stirring rod (22) is additionally provided with straight tube (24) on the bottle cap (2), and the shaft (25) of the motor (21) is arranged in the bottle
It covers in (2), seal pipe (23) is arranged on the stirring rod (22), the top of the stirring rod (22) is located in straight tube (24),
Connecting tube (26) is equipped with below the shaft (25) of the motor (21), the shaft (25) of the motor (21) passes through connecting tube (26)
It is connected with the stirring rod (22).
5. a kind of microbial degradation respirometer as claimed in claim 4, it is characterised in that：The absorption bottle (16) is located at reaction
On conduit (15) between bottle (3) and metering pool (6), the absorption bottle (16) is equipped with air inlet (27) and gas outlet (28), and
Conduit (15) at the absorption bottle (16) in fracture shape and forms two fractures, wherein a fracture connects the absorption bottle
(16) air inlet (27), another fracture connect the gas outlet (28) of the absorption bottle (16), are loaded in the absorption bottle (16)
It is useful for the alkaline solution of absorbing carbon dioxide.
6. such as a kind of microbial degradation respirometer of 3 or 5 any one of them of claim, it is characterised in that：The alkaline solution
For sodium hydroxide or potassium hydroxide, the bottle cap (2) is surrounded by sealing ring.
7. a kind of microbial degradation respirometer as described in claim 1, it is characterised in that：Oxygen cylinder (31) is further included, it is described
One end of conduit (15) connects the gas-guide tube (4) of the reaction bulb (3), and the other end connects the air outlet pipe (11) of metering pool (6), and
The air inlet pipe (12) of the metering pool (6) connects oxygen cylinder (31) to carry out aerobic reaction, the gas-guide tube of the reaction bulb (3)
(4) gas is equipped between oxygen cylinder (31) and arranges (32).
8. a kind of microbial degradation respirometer as described in claim 1, it is characterised in that：Gas collection bag (29) is further included,
One end of the conduit (15) connects the gas-guide tube (4) of the reaction bulb (3), and the other end connects the air inlet of the metering pool (6)
Manage (12), and the air outlet pipe (11) of the metering pool (6) when connecting gas collection bag (29) to carry out anaerobic reaction.
9. a kind of microbial degradation respirometer as described in claim 1, it is characterised in that：Inclined pipe (9) connection second
The end of vertical pivot pipe (8) is fixed on the madial wall of metering pool (6), the first vertical pivot pipe (7) and the second vertical pivot pipe (8) it is upper
It fixes and seals between the inner top surface of port and the metering pool (6).
10. a kind of microbial degradation respirometer as described in claim 1, it is characterised in that：The air inlet pipe of the metering pool (6)
(12) and in air outlet pipe (11) it is fixed by nut (30) that nut (30), the air inlet pipe (12) and air outlet pipe (11) are arranged with
At the top of metering pool (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711465965.8A CN108072586A (en) | 2017-12-28 | 2017-12-28 | A kind of microbial degradation respirometer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711465965.8A CN108072586A (en) | 2017-12-28 | 2017-12-28 | A kind of microbial degradation respirometer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108072586A true CN108072586A (en) | 2018-05-25 |
Family
ID=62156024
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711465965.8A Pending CN108072586A (en) | 2017-12-28 | 2017-12-28 | A kind of microbial degradation respirometer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108072586A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0304318A2 (en) * | 1987-08-21 | 1989-02-22 | Novalal Plc | Production of 2-methyl-5-isopropylhexa-2,5-dien-1-al and of 2-methyl-5-isopropyl-hexa-2,4-dien-1-al in microorganisms |
| CN203824989U (en) * | 2014-05-05 | 2014-09-10 | 湖北洛克泰克仪器有限公司 | Microbial degradation respirometer |
| CN104880504A (en) * | 2015-06-23 | 2015-09-02 | 上海化工研究院 | Pressure difference final motion liquid column balance type coulometry BOD measuring device |
| CN106769856A (en) * | 2017-01-09 | 2017-05-31 | 中国科学院理化技术研究所 | Microbial compost degradation testing device and use method thereof |
| CN207850860U (en) * | 2017-12-28 | 2018-09-11 | 湖北洛克泰克仪器股份有限公司 | A kind of microbial degradation respirometer |
-
2017
- 2017-12-28 CN CN201711465965.8A patent/CN108072586A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0304318A2 (en) * | 1987-08-21 | 1989-02-22 | Novalal Plc | Production of 2-methyl-5-isopropylhexa-2,5-dien-1-al and of 2-methyl-5-isopropyl-hexa-2,4-dien-1-al in microorganisms |
| CN203824989U (en) * | 2014-05-05 | 2014-09-10 | 湖北洛克泰克仪器有限公司 | Microbial degradation respirometer |
| CN104880504A (en) * | 2015-06-23 | 2015-09-02 | 上海化工研究院 | Pressure difference final motion liquid column balance type coulometry BOD measuring device |
| CN106769856A (en) * | 2017-01-09 | 2017-05-31 | 中国科学院理化技术研究所 | Microbial compost degradation testing device and use method thereof |
| CN207850860U (en) * | 2017-12-28 | 2018-09-11 | 湖北洛克泰克仪器股份有限公司 | A kind of microbial degradation respirometer |
Non-Patent Citations (1)
| Title |
|---|
| 《单元整合与测评》编写组: "《单元整合与测评·化学 九年级 上 人教版》", 31 August 2015, 湖南教育出版社, pages: 65 - 66 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107098467A (en) | A kind of built-in anaerobic membrane bioreactor of integral type | |
| CN209537100U (en) | Novel hollow fiber membrane aeration and biological reactor | |
| CN207850860U (en) | A kind of microbial degradation respirometer | |
| CN205146261U (en) | A seal reaction bottle for test of methane potentiality | |
| CN106066198B (en) | Simple device and method for measuring volume of methane in gas collecting bag | |
| CN108072586A (en) | A kind of microbial degradation respirometer | |
| CN110568139A (en) | Water quality detection device | |
| CN202741116U (en) | Photocatalytic reactor | |
| CN206337270U (en) | A kind of pharmaceutical purpose biological fermentation device | |
| CN202599844U (en) | Hydride generation system used for element speciation analysis | |
| CN201577335U (en) | Plant population photosynthesis and dark respiration measuring box | |
| CN112842403B (en) | Collection of aquatic animal breath release14CO2And method of use thereof | |
| CN107063922A (en) | A kind of full-automatic Biochemical Methane Potential test system | |
| CN108120681A (en) | A kind of measurement HO2Transfer efficiency and RO2The apparatus and method for disturbing size | |
| CN203824989U (en) | Microbial degradation respirometer | |
| CN110195008B (en) | Bioreactor for improving mass transfer efficiency of reaction system by utilizing tail gas | |
| CN208679098U (en) | A kind of Chemistry Teaching of Senior High School gas generating unit | |
| CN2505842Y (en) | Water quality on line detecting device | |
| CN206601310U (en) | A kind of full-automatic Biochemical Methane Potential test system | |
| CN208049729U (en) | A kind of chemical experiment medicament anti-blocking mixing device | |
| CN218512122U (en) | Sampling device to sewage treatment plant greenhouse gas discharges | |
| CN215833357U (en) | Reaction concentration detection device for viscosity regulator | |
| CN216144783U (en) | Pure water sodium ion flow-through cell | |
| CN221192029U (en) | Composting fermentation machine | |
| CN219670494U (en) | Reaction vessel for aerobic respiration of microorganisms and respiration amount measurement system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |