CN108070639A - 数字pcr方法快速检测羊水细胞非整倍体 - Google Patents
数字pcr方法快速检测羊水细胞非整倍体 Download PDFInfo
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- CN108070639A CN108070639A CN201711427389.8A CN201711427389A CN108070639A CN 108070639 A CN108070639 A CN 108070639A CN 201711427389 A CN201711427389 A CN 201711427389A CN 108070639 A CN108070639 A CN 108070639A
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- chromosome
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Primer名称* | 序列(5'to3')* |
EIF2C1-F | GTTCGGCTTTCACCAGTC |
EIF2C1-R | CTCCATAGCTCTCCCCACT |
EIF2C1-Taqman probe | CGCCCTGCCATGTGGAAGA |
MBNL2-F | CTCACCTATCCACAATGCA |
MBNL2-R | GGGATTCAAGCGAATTAAC |
MBNL2-Taqman probe | AGGTGCATCATGGGAACGG |
EHZF-F | CCAGCTGGTACTTGGAAGA |
EHZF-R | TGTCGTATGTGGAGCCAA |
EHZF-Taqman probe | TCAGTGCCTGCCTGGTTCC |
DSCR4-F | CCCTTTCCACTCAGAACCA |
DSCR4-R | AGCCCATGGAGGTAAAGTT |
DSCR4-Taqman probe | CAGCAATTTTGGGCAAAATGT |
Noncoding-F | GATGAGGAAGGCAATGAT |
Noncoding-R | TTGGCTTTTACCAAATAG |
Noncoding--Taqman probe | TGTTTCTCTCTGCCTGCACT |
SRY-F | CGCTTAACATAGCAGAAGC |
SRY-R | AGTTTCGAACTCTGGCAC |
SRY-Taqman probe | TGTCGCACTCTCCTTGTTTTTG |
Claims (8)
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CN201711427389.8A CN108070639A (zh) | 2017-12-26 | 2017-12-26 | 数字pcr方法快速检测羊水细胞非整倍体 |
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CN201711427389.8A CN108070639A (zh) | 2017-12-26 | 2017-12-26 | 数字pcr方法快速检测羊水细胞非整倍体 |
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CN108070639A true CN108070639A (zh) | 2018-05-25 |
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CN201711427389.8A Pending CN108070639A (zh) | 2017-12-26 | 2017-12-26 | 数字pcr方法快速检测羊水细胞非整倍体 |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113462768A (zh) * | 2021-07-29 | 2021-10-01 | 中国医学科学院整形外科医院 | 一种利用ddPCR检测小耳畸形患者ECR区域的拷贝数的引物及试剂盒 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20110312503A1 (en) * | 2010-01-23 | 2011-12-22 | Artemis Health, Inc. | Methods of fetal abnormality detection |
CN103160594A (zh) * | 2013-04-03 | 2013-06-19 | 中国科学院苏州纳米技术与纳米仿生研究所 | 基于疏水芯片的多重pcr方法 |
CN105695567A (zh) * | 2015-11-30 | 2016-06-22 | 北京昱晟达医疗科技有限公司 | 一种用于检测胎儿染色体非整倍体的试剂盒、引物和探针序列及检测方法 |
-
2017
- 2017-12-26 CN CN201711427389.8A patent/CN108070639A/zh active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20110312503A1 (en) * | 2010-01-23 | 2011-12-22 | Artemis Health, Inc. | Methods of fetal abnormality detection |
CN103160594A (zh) * | 2013-04-03 | 2013-06-19 | 中国科学院苏州纳米技术与纳米仿生研究所 | 基于疏水芯片的多重pcr方法 |
CN105695567A (zh) * | 2015-11-30 | 2016-06-22 | 北京昱晟达医疗科技有限公司 | 一种用于检测胎儿染色体非整倍体的试剂盒、引物和探针序列及检测方法 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113462768A (zh) * | 2021-07-29 | 2021-10-01 | 中国医学科学院整形外科医院 | 一种利用ddPCR检测小耳畸形患者ECR区域的拷贝数的引物及试剂盒 |
CN113462768B (zh) * | 2021-07-29 | 2023-05-30 | 中国医学科学院整形外科医院 | 一种利用ddPCR检测小耳畸形患者ECR区域的拷贝数的引物及试剂盒 |
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Inventor after: Liu Jinxiu Inventor after: Xu Ming Inventor after: Liu Changsheng Inventor after: Duan Wenyuan Inventor after: Chen Shoulin Inventor after: Chen Jie Inventor after: Dou Yanyan Inventor after: Meng Zhenxiang Inventor after: Yan Jianmei Inventor after: Zhang Qianqian Inventor after: Yang Haixing Inventor before: Liu Jinxiu Inventor before: Duan Wenyuan Inventor before: Chen Shoulin Inventor before: Chen Jie Inventor before: Dou Yanyan Inventor before: Meng Zhenxiang Inventor before: Yan Jianmei Inventor before: Zhang Qianqian Inventor before: Yang Haixing |
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