CN108065138A - A kind of Lotus Leafextract feed addictive used for aquiculture and its manufacturing method - Google Patents
A kind of Lotus Leafextract feed addictive used for aquiculture and its manufacturing method Download PDFInfo
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- CN108065138A CN108065138A CN201810065935.6A CN201810065935A CN108065138A CN 108065138 A CN108065138 A CN 108065138A CN 201810065935 A CN201810065935 A CN 201810065935A CN 108065138 A CN108065138 A CN 108065138A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/14—Pretreatment of feeding-stuffs with enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention discloses a kind of Lotus Leafextract feed addictive used for aquiculture and its manufacturing method, contain micro 0.2 part of 0.3 part of slightly solubility lotus leaf alkaloid, 1.5 parts of 2 parts of lotus leaf Huang paulownias, 0.02 part 0.04 part of phytic acid, ammonium chloride, 3 parts 4 parts of vegetable oil, 15 parts 20 parts of bed-silt in the Lotus Leafextract feed addictive by weight.The present invention compared to existing technologies, can improve aquatic products meat, promoted aquatic products from immunity, derive from a wealth of sources, palatability is good, applied widely.
Description
Technical field
The present invention relates to aquaculture field more particularly to a kind of Lotus Leafextract feed addictive used for aquiculture and its
Manufacturing method.
Background technology
Feed is the general name of the food of owner domesticated animal, compares narrowly general feeds and generally refers to agricultural
Or the food of animal husbandry domesticated animal.Feed (Feed) include soybean, dregs of beans, corn, fish meal, amino acid, miscellaneous dregs of rice, additive,
The feedstuff of more than ten kinds such as whey powder, grease, meat meal tankage, cereal, sugar grass.
In existing aquatic products conventional feed, the aquatic products for food industry (such as Fish protein and dried fish floss) is usually that full feed is fed
It supports, meat is poor, easily turns over the pool, and in the prior art also without being specialized in for food industry with aquatic feeds.
Therefore, be badly in need of on the market a kind of improvement aquatic products meat, promoted aquatic products from immunity, derive from a wealth of sources, palatability is good, suitable
With the wide Lotus Leafextract feed addictive used for aquiculture of scope and its manufacturing method.
The content of the invention
In view of the above defects of the prior art, the present invention is intended to provide a kind of improvement aquatic products meat, promotion aquatic products
From immunity, derive from a wealth of sources, the Lotus Leafextract feed addictive used for aquiculture that palatability is good, applied widely and its manufacture
Method.
To achieve these goals, the present invention uses following technical scheme:A kind of Lotus Leafextract feed used for aquiculture
The manufacturing method of additive, comprises the following steps:
1) prepare before production
1. raw material prepare:Prepare 100 parts -120 parts of the Hunan white lotus lotus leaf of fresh clean by weight;
2. process materials prepare:Prepare hydrochloric acid solution, the X-5 macroreticular resins of enough Solute mass fractions 10% by weight
10 parts -15 parts, the cellulase aqueous solution of enough mass fractions 1%, enough absolute ethyl alcohols, enough sodium hydroxides, enough ammonium hydroxide,
Enough chloroforms, enough vegetable oil, enough bed-silts for being added with 1% mass fraction ethyl alcohol;
2) alkaloid and phytic acid extraction
1. the Hunan white lotus lotus leaf that step in 1) 1. prepares is scrubbed clean using the absolute ethyl alcohol that step in 1) 2. prepares, so
It is steamed afterwards from the moisture content in absolute ethyl alcohol and Hunan white lotus lotus leaf, obtains clean dried lotus leaf;
2. by 1. clean dried lotus leaf mechanical crushing that step obtains into the lotus leaf fragment of size 1mm-2mm, then by lotus
In the cellulase aqueous solution that 1) leaf fragment is completely immersed in 2. step prepares, 2h-3h is impregnated, obtains enzymolysis liquid;
3. 1) in 2. enzymolysis liquid that 2. ammonium hydroxide dispensing that step in prepares is obtained to step, while it is slowly stirred enzymolysis
Liquid, until solution PH 9-10, Solid content, remaining alkaline solution are filtered out after continuing 10min-15min;
4. the chloroform that in being added dropwise 1) in the alkaline solution 3. obtained in step 2. step prepares is alkaline to chloroform volume and original
After liquor capacity ratio is 1: 5, apply the ultrasonic activation of 100W-200W, then standing separation, intercepts and abandon chloroform, retains
Aqueous solution;
5. the hydrochloric acid solution that in being added dropwise 1) in 4. aqueous solution that step is obtained 2. step prepares is to solution PH 5.5-6, quiet
Ammonium hydroxide that in being added dropwise 1) after lasting 10min-15min 2. step prepares is put to pH value 7-8, the sediment of acquisition be needed for biology
Alkali steams the surplus solution of acquisition from obtaining phytic acid and ammonium salt mixture after moisture content;
3) lotus flavone extracts
1. by the stage 2) Solid content that 3. filters out of step be completely immersed in 1) in the absolute ethyl alcohol that 2. prepares of step, be heated up to
50-70 DEG C, to the ultrasonic activation of the after-applied 100W-200W of temperature, 15min-20min is kept, obtains alcohol extract;
2. 1) 2. X-5 macroreticular resins that step in prepares are completely immersed in 1. alcohol extract that step obtains, it is slowly stirred
Alcohol extract continues 0.5h-1h, then takes out the X-5 macroreticular resins for completing absorption;
3. the X-5 macroreticular resins that 2. completion that step is obtained is adsorbed are steamed using anhydrous ethanol elution from eluent
Ethyl alcohol and moisture content, i.e. lotus flavone needed for acquisition;
4) prepared by Lotus Leafextract feed addictive
1. by the stage 2) step 5. obtain alkaloid, phytic acid and ammonium salt mixture, the stage 3) lotus leaf that 3. obtains of step
Flavones is uniformly mixed, and obtains mixture, and by it, 3 parts -4 parts of vegetable oil is uniformly mixed with parts by weight, then will be enclosed with again mixed
The bed-silt for closing 15 parts -20 parts of vegetable oil and the parts by weight of object is uniformly mixed, that is, obtain needed for lotus leaf used for aquiculture extract
Object feed addictive.
The Lotus Leafextract feed addictive used for aquiculture prepared using the above method, inside contains 0.2 by weight
- 0.3 part of slightly solubility lotus leaf alkaloid of part, 1.5 parts of -2 parts of lotus leaf Huang paulownias, 0.02 part -0.04 part of phytic acid, ammonium chloride are micro, plant
Oily 3 parts -4 parts, 15 parts -20 parts of bed-silt.
The present invention application method be:It is added to the mass fraction of 20%-25% in the feed fed every time, from aquatic products
Start to maintain to use always during feeding fiber food until fishing.
Compared with the prior art, the present invention has the following advantages as a result of said program:(1) present invention uses
Lotus leaf almost useless in planting industry for raw material (device shape good lotus leaf in part may be used as making lotus leaf meal, but
Other lotus leaves are difficult useful, mostly based on discarded), good economy performance, and also it is only high-precision using high cost in the prior art
Degree extracting mode prepares the correlation technique of Lotus Leafextract, and cost is excessively high and is normally only used for, as health products, never having it
There is directly addition lotus leaf end as the purposes of feed addictive, and in routine techniques into feed, component content therein
Purity is too low and solid lock is in cell, it is difficult to be digested absorption.(2) mouthfeel improved by micro active phytic acid, promote to digest,
The palatability of the present invention is considerably increased, adds application range.(3) lotus flavone can be obviously improved the meat of aquatic products, make it
Naturally soft, mouthfeel is promoted, although to having used the aquatic products grown naturally in the aquatic products and rivers of the forage feed of the present invention still
So there is the gap in mouthfeel, but it is enough applied to food mouth industry improvement mouthfeel, and the present invention can reduce aquatic products body nuisancelessly
Fat content, the average body fat content (being compared with the fish of the same race of pure forage feed) that can about reduce 20%-25%, simultaneously because lotus leaf
Flavones can mention anti-inflammatory, the effect of antibacterial, therefore apply the use that can slightly subtract immune formulation after the present invention, on the one hand reduce
Nursing cost, on the other hand decrease aquatic products it is edible when harm.(4) Nuciferine is a kind of aporphine type life in lotus leaf
Alkaloids are the main Lipid-lowering activities ingredient in lotus leaf, have effect for reducing blood fat, Green Tea Extract, inhibit hypercholesterolemia and artery
Medications, the dietotherapy effects such as hardening, but also with antimitotic effect, have stronger fungistatic effect, to hundred profit of animal body
And none does harm to, and is very excellent additive.(5) by the slightly solubility Nuciferine being obtained in particular in lotus leaf, coordinate vegetable oil and
Mud makes the present invention have excellent slow releasing function, according to experiment, the present invention -20 days about 15 days cycle of sustained release, lasting effect
It is good, the demand of aquaculture preferably.(6) according to investigating applicant more with Experimental Comparison many times, using Hunan white lotus lotus leaf
The effective ingredient of extraction is preferably for feed, easily absorbs, it is with obvious effects to improve meat.
Specific embodiment
Embodiment 1:
A kind of Lotus Leafextract feed addictive used for aquiculture is inside given birth to containing 0.2Kg slightly solubilities lotus leaf by weight
Alkaloids, 1.5Kg lotus leaf Huangs paulownia, phytic acid 0.02Kg, ammonium chloride are micro, vegetable oil 4Kg, bed-silt 20Kg.
The manufacturing method of above-mentioned Lotus Leafextract feed addictive used for aquiculture, comprises the following steps:
1) prepare before production
1. raw material prepare:Prepare the Hunan white lotus lotus leaf 100Kg of fresh clean by weight;
2. process materials prepare:Prepare hydrochloric acid solution, the X-5 macroreticular resins of enough Solute mass fractions 10% by weight
It is 10Kg, the cellulase aqueous solution of enough mass fractions 1%, enough absolute ethyl alcohols, enough sodium hydroxides, enough ammonium hydroxide, enough
Chloroform, enough vegetable oil, enough bed-silts added with 1% mass fraction ethyl alcohol;
2) alkaloid and phytic acid extraction
1. the Hunan white lotus lotus leaf that step in 1) 1. prepares is scrubbed clean using the absolute ethyl alcohol that step in 1) 2. prepares, so
It is steamed afterwards from the moisture content in absolute ethyl alcohol and Hunan white lotus lotus leaf, obtains clean dried lotus leaf;
2. by 1. clean dried lotus leaf mechanical crushing that step obtains into the lotus leaf fragment of size 1mm-2mm, then by lotus
In the cellulase aqueous solution that 1) leaf fragment is completely immersed in 2. step prepares, 2h is impregnated, obtains enzymolysis liquid;
3. 1) in 2. enzymolysis liquid that 2. ammonium hydroxide dispensing that step in prepares is obtained to step, while it is slowly stirred enzymolysis
Liquid, until solution PH 9, Solid content, remaining alkaline solution are filtered out after continuing 10min;
4. the chloroform that in being added dropwise 1) in the alkaline solution 3. obtained in step 2. step prepares is alkaline to chloroform volume and original
After liquor capacity ratio is 1: 5, apply the ultrasonic activation of 100W, then standing separation, intercepts and abandon chloroform, retains water-soluble
Liquid;
5. the hydrochloric acid solution that in being added dropwise 1) in 4. aqueous solution that step is obtained 2. step prepares is stood to solution PH 5.5
For the ammonium hydroxide that in being added dropwise 1) after lasting 10min 2. step prepares to pH value 7, the sediment of acquisition is required alkaloid, will be obtained
Surplus solution steam from obtaining phytic acid and ammonium salt mixture after moisture content;
3) lotus flavone extracts
1. by the stage 2) Solid content that 3. filters out of step be completely immersed in 1) in the absolute ethyl alcohol that 2. prepares of step, be heated up to
50 DEG C, to the ultrasonic activation of the after-applied 100W of temperature, 15min is kept, obtains alcohol extract;
2. 1) 2. X-5 macroreticular resins that step in prepares are completely immersed in 1. alcohol extract that step obtains, it is slowly stirred
Alcohol extract continues 0.5h, then takes out the X-5 macroreticular resins for completing absorption;
3. the X-5 macroreticular resins that 2. completion that step is obtained is adsorbed are steamed using anhydrous ethanol elution from eluent
Ethyl alcohol and moisture content, i.e. lotus flavone needed for acquisition;
4) prepared by Lotus Leafextract feed addictive
1. by the stage 2) step 5. obtain alkaloid, phytic acid and ammonium salt mixture, the stage 3) lotus leaf that 3. obtains of step
Flavones is uniformly mixed, and obtains mixture, it with the vegetable oil of parts by weight 4Kg is uniformly mixed, then will be enclosed with mixture again
Vegetable oil be uniformly mixed with the bed-silt of parts by weight 20Kg, that is, obtain needed for Lotus Leafextract feed used for aquiculture addition
Agent.
According to the feed addictive that the present embodiment produces, with 20% mass fraction additive amount, fed for black carp feed
When supporting, fish is cultivated as negative control using the feed that the present invention is not used, averagely reduces body fat 16%, mouthfeel has slight improvement, puts down
15% is increased weight, without using enhancing immune formulation.
Embodiment 2:
It is whole consistent with embodiment 1, it is in place of difference:
A kind of Lotus Leafextract feed addictive used for aquiculture is inside given birth to containing 0.3Kg slightly solubilities lotus leaf by weight
Alkaloids, 2Kg lotus leaf Huangs paulownia, phytic acid 0.04Kg, ammonium chloride are micro, vegetable oil 3Kg, bed-silt 15Kg.
The manufacturing method of above-mentioned Lotus Leafextract feed addictive used for aquiculture, comprises the following steps:
1) prepare before production
1. raw material prepare:Prepare the Hunan white lotus lotus leaf 120Kg of fresh clean by weight;
2. process materials prepare:Prepare hydrochloric acid solution, the X-5 macroreticular resins of enough Solute mass fractions 10% by weight
It is 15Kg, the cellulase aqueous solution of enough mass fractions 1%, enough absolute ethyl alcohols, enough sodium hydroxides, enough ammonium hydroxide, enough
Chloroform, enough vegetable oil, enough bed-silts added with 1% mass fraction ethyl alcohol;
2) alkaloid and phytic acid extraction
2. by 1. clean dried lotus leaf mechanical crushing that step obtains into the lotus leaf fragment of size 1mm-2mm, then by lotus
In the cellulase aqueous solution that 1) leaf fragment is completely immersed in 2. step prepares, 3h is impregnated, obtains enzymolysis liquid;
3. 1) in 2. enzymolysis liquid that 2. ammonium hydroxide dispensing that step in prepares is obtained to step, while it is slowly stirred enzymolysis
Liquid, until solution PH 10, Solid content, remaining alkaline solution are filtered out after continuing 15min;
4. the chloroform that in being added dropwise 1) in the alkaline solution 3. obtained in step 2. step prepares is alkaline to chloroform volume and original
After liquor capacity ratio is 1: 5, apply the ultrasonic activation of 200W, then standing separation, intercepts and abandon chloroform, retains water-soluble
Liquid;
5. the hydrochloric acid solution that in being added dropwise 1) in 4. aqueous solution that step is obtained 2. step prepares to solution PH 6, hold by standing
The ammonium hydroxide that in being added dropwise 1) after continuous 15min 2. step prepares is to pH value 8, and the sediment of acquisition is required alkaloid, by acquisition
Surplus solution is steamed from obtaining phytic acid and ammonium salt mixture after moisture content;
3) lotus flavone extracts
1. by the stage 2) Solid content that 3. filters out of step be completely immersed in 1) in the absolute ethyl alcohol that 2. prepares of step, be heated up to
70 DEG C, to the ultrasonic activation of the after-applied 200W of temperature, 20min is kept, obtains alcohol extract;
2. 1) 2. X-5 macroreticular resins that step in prepares are completely immersed in 1. alcohol extract that step obtains, it is slowly stirred
Alcohol extract continues 1h, then takes out the X-5 macroreticular resins for completing absorption;
4) prepared by Lotus Leafextract feed addictive
1. by the stage 2) step 5. obtain alkaloid, phytic acid and ammonium salt mixture, the stage 3) lotus leaf that 3. obtains of step
Flavones is uniformly mixed, and obtains mixture, it with the vegetable oil of parts by weight 3Kg is uniformly mixed, then will be enclosed with mixture again
Vegetable oil be uniformly mixed with the bed-silt of parts by weight 15Kg, that is, obtain needed for Lotus Leafextract feed used for aquiculture addition
Agent.
According to the feed addictive that the present embodiment produces, with 25% mass fraction additive amount, fed for black carp feed
When supporting, fish being cultivated as negative control using the feed that the present invention is not used, averagely reduces body fat 25%, mouthfeel has more apparent improvement,
Average weight gain 23%, without using enhancing immune formulation.
The foregoing description of the disclosed embodiments, only for professional and technical personnel in the field is enable to realize or use this
Invention.A variety of modifications of these embodiments will be apparent for those skilled in the art, institute herein
The General Principle of definition can be realized in other embodiments without departing from the spirit or scope of the present invention.Therefore,
The present invention is not intended to be limited to the embodiments shown herein, and is to fit to special with principles disclosed herein and novelty
The consistent most wide scope of point.
Claims (2)
1. a kind of manufacturing method of Lotus Leafextract feed addictive used for aquiculture, it is characterised in that comprise the following steps:
1) prepare before production
1. raw material prepare:Prepare 100 parts -120 parts of the Hunan white lotus lotus leaf of fresh clean by weight;
2. process materials prepare:Prepare hydrochloric acid solution, the X-5 macroreticular resins 10 of enough Solute mass fractions 10% by weight
- 15 parts of part, the cellulase aqueous solution of enough mass fractions 1%, enough absolute ethyl alcohols, enough sodium hydroxides, enough ammonium hydroxide, foot
Chloroform, enough vegetable oil, enough bed-silt of the amount added with 1% mass fraction ethyl alcohol;
2) alkaloid and phytic acid extraction
1. the Hunan white lotus lotus leaf that step in 1) 1. prepares is scrubbed clean using the absolute ethyl alcohol that step in 1) 2. prepares, Ran Houzheng
From the moisture content in absolute ethyl alcohol and Hunan white lotus lotus leaf, clean dried lotus leaf is obtained;
It is 2. then that lotus leaf is broken by 1. clean dried lotus leaf mechanical crushing that step obtains into the lotus leaf fragment of size 1mm-2mm
In the cellulase aqueous solution that 1) piece is completely immersed in 2. step prepares, 2h-3h is impregnated, obtains enzymolysis liquid;
3. in 2. enzymolysis liquid that the ammonium hydroxide dispensing that step in 1) 2. prepares is obtained to step, while enzymolysis liquid is slowly stirred, until
Solution PH 9-10 filters out Solid content, remaining alkaline solution after continuing 10min-15min;
4. the chloroform that in being added dropwise 1) in the alkaline solution 3. obtained in step 2. step prepares is to chloroform volume and former alkaline solution
After volume ratio is 1: 5, apply the ultrasonic activation of 100W-200W, then standing separation, intercept and abandon chloroform, retain water-soluble
Liquid;
5. the hydrochloric acid solution that in being added dropwise 1) in 4. aqueous solution that step is obtained 2. step prepares to solution PH 5.5-6, standing is held
The ammonium hydroxide that in being added dropwise 1) after continuous 10min-15min 2. step prepares to pH value 7-8, the sediment of acquisition be needed for alkaloid,
The surplus solution of acquisition is steamed from obtaining phytic acid and ammonium salt mixture after moisture content;
3) lotus flavone extracts
1. by the stage 2) Solid content that 3. filters out of step be completely immersed in 1) in the absolute ethyl alcohol that 2. prepares of step, be heated up to 50-
70 DEG C, to the ultrasonic activation of the after-applied 100W-200W of temperature, 15min-20min is kept, obtains alcohol extract;
2. 1) 2. X-5 macroreticular resins that step in prepares are completely immersed in 1. alcohol extract that step obtains, alcohol extracting is slowly stirred
Liquid continues 0.5h-1h, then takes out the X-5 macroreticular resins for completing absorption;
3. the X-5 macroreticular resins that 2. completion that step is obtained is adsorbed are steamed using anhydrous ethanol elution from the ethyl alcohol in eluent
And moisture content, that is, obtain needed for lotus flavone;
4) prepared by Lotus Leafextract feed addictive
1. by the stage 2) step 5. obtain alkaloid, phytic acid and ammonium salt mixture, the stage 3) lotus flavone that 3. obtains of step
It is uniformly mixed, obtains mixture, 3 parts -4 parts of vegetable oil is uniformly mixed with parts by weight by it, then will be enclosed with mixture again
The bed-silts of 15 parts -20 parts of vegetable oil and parts by weight be uniformly mixed, that is, obtain needed for Lotus Leafextract used for aquiculture raise
Feed additives.
2. a kind of Lotus Leafextract feed addictive used for aquiculture prepared using claim 1 the method, feature are existed
In:By weight containing 0.2 part of -0.3 part of slightly solubility lotus leaf alkaloid, 1.5 parts of -2 parts of lotus leaf Huang paulownias, plant in the feed addictive
0.02 part -0.04 part of acid, ammonium chloride are micro, 3 parts -4 parts of vegetable oil, 15 parts -20 parts of bed-silt.
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CN201810065935.6A CN108065138A (en) | 2018-01-22 | 2018-01-22 | A kind of Lotus Leafextract feed addictive used for aquiculture and its manufacturing method |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109601706A (en) * | 2019-01-30 | 2019-04-12 | 华中农业大学 | A kind of compound additive and preparation method and application improving Growth of Grass Carps Ctenopharyngodon Idellus and health |
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CN103626770A (en) * | 2013-11-27 | 2014-03-12 | 大兴安岭嘉迪欧营养原料有限公司 | Novel method for efficiently extracting total alkaloids from wild thermopsis lanceolata in Greater Khingan Mountains |
CN105231098A (en) * | 2015-11-10 | 2016-01-13 | 刘雷 | Additive capable of improving fish meat quality as well as preparation method and application of additive |
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2018
- 2018-01-22 CN CN201810065935.6A patent/CN108065138A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103626770A (en) * | 2013-11-27 | 2014-03-12 | 大兴安岭嘉迪欧营养原料有限公司 | Novel method for efficiently extracting total alkaloids from wild thermopsis lanceolata in Greater Khingan Mountains |
CN105231098A (en) * | 2015-11-10 | 2016-01-13 | 刘雷 | Additive capable of improving fish meat quality as well as preparation method and application of additive |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109601706A (en) * | 2019-01-30 | 2019-04-12 | 华中农业大学 | A kind of compound additive and preparation method and application improving Growth of Grass Carps Ctenopharyngodon Idellus and health |
CN109601706B (en) * | 2019-01-30 | 2022-10-04 | 华中农业大学 | Compound additive for improving growth and health of grass carps and preparation method and application thereof |
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