CN108060124A - A kind of adipose tissue extracorporeal treatment liquid - Google Patents
A kind of adipose tissue extracorporeal treatment liquid Download PDFInfo
- Publication number
- CN108060124A CN108060124A CN201711322588.2A CN201711322588A CN108060124A CN 108060124 A CN108060124 A CN 108060124A CN 201711322588 A CN201711322588 A CN 201711322588A CN 108060124 A CN108060124 A CN 108060124A
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- Prior art keywords
- adipose tissue
- treatment liquid
- extracorporeal treatment
- hepes
- remaining
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0653—Adipocytes; Adipose tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Rheumatology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of adipose tissue extracorporeal treatment liquid, include the raw material of following mass fraction：Magnesium sulfate 0.1 5%, calcium chloride 0.1 5%, disodium hydrogen phosphate 0.1 5%, sodium acid carbonate 0.1 5%, hepes 0.1 5%, remaining as physiological saline.The present invention's, adipocyte and stem cell are put into and wherein handle by adipose tissue extracorporeal treatment liquid, can obtain adipocyte in good condition and stem cell, and good adipocyte and stem cell are provided for disease treatment and beauty.
Description
Technical field
The present invention relates to a kind of extracorporeal treatment liquid, and in particular to a kind of adipose tissue extracorporeal treatment liquid.
Background technology
It usually needs to extract autologous adipose tissue and mescenchymal stem cell in terms of present day plastic beauty, utilizes autologous fat
Setup action filler is very safe.Tissue repair and anti-aging etc. are carried out using self mescenchymal stem cell also to obtain well
Effect.But how the adipose tissue taken out and stem cell allow it to reach good state by appropriate processing in vitro, from
And it is also a crucial technology to reach better effect.
The content of the invention
The technical problems to be solved by the invention be the adipose tissue that takes out and stem cell when taking out external, state is not
It is good, subsequent operation is easily influenced, and it is an object of the present invention to provide a kind of adipose tissue extracorporeal treatment liquid, adipocyte and stem cell are put
Enter and wherein handled, adipocyte in good condition and stem cell can be obtained, provided for disease treatment and beauty good
Adipocyte and stem cell.
The present invention is achieved through the following technical solutions：
A kind of adipose tissue extracorporeal treatment liquid includes the raw material of following mass fraction：Magnesium sulfate 0.1-5%, calcium chloride
0.1-5%, disodium hydrogen phosphate 0.1-5%, sodium acid carbonate 0.1-5%, hepes 0.1-5%, remaining as physiological saline.
A kind of adipose tissue extracorporeal treatment liquid includes the raw material of following mass fraction：Magnesium sulfate 1-4%, calcium chloride 2-
5%th, disodium hydrogen phosphate 0.5-3%, sodium acid carbonate 1-4%, hepes 2-5%, remaining as physiological saline.
A kind of adipose tissue extracorporeal treatment liquid includes the raw material of following mass fraction：Magnesium sulfate 2%, calcium chloride 3%, phosphorus
Sour disodium hydrogen 3.5%, sodium acid carbonate 2%, hepes 4%, remaining as physiological saline.
A kind of adipose tissue extracorporeal treatment liquid includes the raw material of following mass fraction：Magnesium sulfate 0.1%, calcium chloride
0.1%th, disodium hydrogen phosphate 0.1%, sodium acid carbonate 0.1%, hepes 0.1%, remaining as physiological saline.
A kind of adipose tissue extracorporeal treatment liquid includes the raw material of following mass fraction：Magnesium sulfate 5%, calcium chloride 5%, phosphorus
Sour disodium hydrogen 5%, sodium acid carbonate 5%, hepes 5%, remaining as physiological saline.
Compared with prior art, the present invention it has the following advantages and advantages：
A kind of adipose tissue extracorporeal treatment liquid of the present invention, adipocyte and stem cell are put into and wherein handled, can
Adipocyte in good condition and stem cell are obtained, good adipocyte and stem cell are provided for disease treatment and beauty.
Specific embodiment
Understand to make the object, technical solutions and advantages of the present invention clearer, with reference to embodiment, the present invention is made
Further to be described in detail, exemplary embodiment of the invention and its explanation are only used for explaining the present invention, are not intended as to this
The restriction of invention.
Embodiment 1
A kind of adipose tissue extracorporeal treatment liquid includes the raw material of following mass fraction：Magnesium sulfate 2%, calcium chloride 3%, phosphorus
Sour disodium hydrogen 3.5%, sodium acid carbonate 2%, hepes 4%, remaining as physiological saline.
Embodiment 2
A kind of adipose tissue extracorporeal treatment liquid includes the raw material of following mass fraction：Magnesium sulfate 0.1%, calcium chloride
0.1%th, disodium hydrogen phosphate 0.1%, sodium acid carbonate 0.1%, hepes 0.1%, remaining as physiological saline.
Embodiment 3
A kind of adipose tissue extracorporeal treatment liquid includes the raw material of following mass fraction：Magnesium sulfate 5%, calcium chloride 5%, phosphorus
Sour disodium hydrogen 5%, sodium acid carbonate 5%, hepes 5%, remaining as physiological saline.
Embodiment 4
Experimental group：Using the treatment fluid of the embodiment of the present invention 1；
Control group：Using physiological saline.
Adipose tissue and derived mesenchymal stem cells in vitro processing method are simple：1) the normal person's fat extracted through 1.8mm syringe needles
Tissue is according to 5：2 volume ratio adds in the experimental group tissue treatment liquid (experimental group) being sterile filtered and physiological saline (contrast groups)
In, mixing stood in 37 DEG C 2 it is small when complete the extracorporeal treatment of adipose tissue and stem cell.
2) will through experimental group and contrast groups, treated that adipose tissue liquid respectively takes a drop, stand 15 minutes；Cell is gathered to shine
Piece.
Experiment conclusion：Fat tissue cell and stem cell when the tissue treatment liquid processing 2 of the present invention is small after cell surface
It is smooth mellow and full full；And cell shape is irregular after only being handled two hours with physiological saline, and there is gauffer in surface.
Above-described specific embodiment has carried out the purpose of the present invention, technical solution and advantageous effect further
It is described in detail, it should be understood that the foregoing is merely the specific embodiments of the present invention, is not intended to limit the present invention
Protection domain, within the spirit and principles of the invention, any modification, equivalent substitution, improvement and etc. done should all include
Within protection scope of the present invention.
Claims (5)
1. a kind of adipose tissue extracorporeal treatment liquid, which is characterized in that include the raw material of following mass fraction：Magnesium sulfate 0.1-5%,
Calcium chloride 0.1-5%, disodium hydrogen phosphate 0.1-5%, sodium acid carbonate 0.1-5%, hepes 0.1-5%, remaining as physiological saline.
2. a kind of adipose tissue extracorporeal treatment liquid according to claim 1, which is characterized in that including following mass fraction
Raw material：Magnesium sulfate 1-4%, calcium chloride 2-5%, disodium hydrogen phosphate 0.5-3%, sodium acid carbonate 1-4%, hepes 2-5% are remaining
For physiological saline.
3. a kind of adipose tissue extracorporeal treatment liquid according to claim 1, which is characterized in that including following mass fraction
Raw material：Magnesium sulfate 2%, calcium chloride 3%, disodium hydrogen phosphate 3.5%, sodium acid carbonate 2%, hepes 4%, remaining as physiology salt
Water.
4. a kind of adipose tissue extracorporeal treatment liquid according to claim 1, which is characterized in that including following mass fraction
Raw material：Magnesium sulfate 0.1%, calcium chloride 0.1%, disodium hydrogen phosphate 0.1%, sodium acid carbonate 0.1%, hepes 0.1%, remaining as
Physiological saline.
5. a kind of adipose tissue extracorporeal treatment liquid according to claim 1, which is characterized in that including following mass fraction
Raw material：Magnesium sulfate 5%, calcium chloride 5%, disodium hydrogen phosphate 5%, sodium acid carbonate 5%, hepes 5%, remaining as physiological saline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711322588.2A CN108060124A (en) | 2017-12-12 | 2017-12-12 | A kind of adipose tissue extracorporeal treatment liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711322588.2A CN108060124A (en) | 2017-12-12 | 2017-12-12 | A kind of adipose tissue extracorporeal treatment liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108060124A true CN108060124A (en) | 2018-05-22 |
Family
ID=62138232
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711322588.2A Withdrawn CN108060124A (en) | 2017-12-12 | 2017-12-12 | A kind of adipose tissue extracorporeal treatment liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108060124A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114557336A (en) * | 2021-11-25 | 2022-05-31 | 中国人民解放军空军军医大学 | Tissue treatment fluid capable of improving primary tissue isolation quantity and activity and preparation method and application thereof |
-
2017
- 2017-12-12 CN CN201711322588.2A patent/CN108060124A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114557336A (en) * | 2021-11-25 | 2022-05-31 | 中国人民解放军空军军医大学 | Tissue treatment fluid capable of improving primary tissue isolation quantity and activity and preparation method and application thereof |
| CN114557336B (en) * | 2021-11-25 | 2023-05-05 | 中国人民解放军空军军医大学 | Tissue treatment fluid capable of improving primary separation quantity and activity of tissues as well as preparation method and application thereof |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20180522 |
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| WW01 | Invention patent application withdrawn after publication |