CN106367320B - Manual fat core cluster cell and fat matrix blood vessel component collector - Google Patents
Manual fat core cluster cell and fat matrix blood vessel component collector Download PDFInfo
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- CN106367320B CN106367320B CN201611022907.3A CN201611022907A CN106367320B CN 106367320 B CN106367320 B CN 106367320B CN 201611022907 A CN201611022907 A CN 201611022907A CN 106367320 B CN106367320 B CN 106367320B
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- 239000011159 matrix material Substances 0.000 title claims abstract description 17
- 210000004204 blood vessel Anatomy 0.000 title claims abstract description 8
- 238000011282 treatment Methods 0.000 claims abstract description 51
- 238000001914 filtration Methods 0.000 claims abstract description 43
- 238000002360 preparation method Methods 0.000 claims abstract description 26
- 230000002792 vascular Effects 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 7
- 238000004140 cleaning Methods 0.000 claims description 11
- 239000007788 liquid Substances 0.000 claims description 8
- 238000003825 pressing Methods 0.000 claims description 7
- 238000007789 sealing Methods 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 4
- 239000002699 waste material Substances 0.000 claims description 3
- 238000000265 homogenisation Methods 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims description 2
- 238000011221 initial treatment Methods 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 13
- 210000000577 adipose tissue Anatomy 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 5
- 210000002808 connective tissue Anatomy 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 3
- 239000003102 growth factor Substances 0.000 abstract description 3
- 230000001172 regenerating effect Effects 0.000 abstract description 3
- 210000000130 stem cell Anatomy 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000008859 change Effects 0.000 abstract description 2
- 230000003712 anti-aging effect Effects 0.000 abstract 1
- 238000007493 shaping process Methods 0.000 abstract 1
- 230000006872 improvement Effects 0.000 description 3
- 230000003416 augmentation Effects 0.000 description 2
- 230000003796 beauty Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 230000032683 aging Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 238000002316 cosmetic surgery Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000017423 tissue regeneration Effects 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M39/00—Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/08—Homogenizing
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Abstract
The invention relates to a manual fat core cluster cell and fat matrix blood vessel component collector which comprises an air filter, a connecting conduit, a luer connector inlet, an inlet one-way valve, a fat inlet filtering chamber, a fat preparation processor, a positioning fixing pin, a positioning clamping groove, a fat outlet filtering chamber, an outlet one-way valve, a luer connector outlet and a three-way change valve. The invention has the beneficial effects that: by filtering unused connective tissue, homogenizing and purifying adipose tissue, the high-quality autologous adipose tissue rich in various growth factors and adipose stem cells is obtained, the immune rejection is reduced, the effects of beautifying, shaping, anti-aging and regenerative medicine treatment are improved, and the safety, effectiveness and durability of the clinical treatment are improved. The method is simple to operate, good in applicability, safe and reliable, and provides a novel and convenient method for collecting autologous adipose tissue core colony cells and adipose matrix vascular components in the biomedical engineering field.
Description
Technical Field
The invention relates to the fields of biomedical engineering and regenerative medical instruments, in particular to a manual fat core clustered cell and fat matrix vascular component collector.
Background
Medical cosmetology and plastic are a series of treatments carried out by medical means including medicines, instruments, operations and the like, so as to achieve the purposes of changing the external form and color of the human body, partially improving the physiological functions of the human body and enhancing the external aesthetic feeling of the human body. With the continuous advancement of medical cosmetology, autologous fat filling technology is favored by more and more consumers. After autologous fat is sucked in the operation, the autologous fat is treated and filled in vitro until the skin is subjected to beauty operations such as wrinkle removal, facial contour improvement, breast augmentation, hip augmentation and the like, and the adipose matrix vascular component has the advantages of no immune rejection, good beauty effect, long retention time and the like, and has wide prospect in the field of tissue regeneration repair treatment at present.
However, the self fat just taken out is entrained with other connective tissues to easily block the needle head, so that the beautifying effect is affected; low fat homogenization degree, low concentration of growth factors and stem cells, and is unfavorable for the use of cosmetic surgery. Accordingly, there is a need for a reasonable improvement over the prior art in view of the above.
Disclosure of Invention
Aiming at the defects, the invention provides a manual fat core cluster cell and fat matrix vascular component collector for obtaining high-quality autologous fat by shaking a fat container tube to filter out unused connective tissue through the design of a fat inlet and outlet filter chamber and the injection and discharge of cleaning fluid and purifying and homogenizing fat.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a manual fat core cluster cell and fat matrix blood vessel component collector comprises a fat cleaning and filtering unit, a fat treatment container unit and a fixing unit;
the fat cleaning and filtering unit comprises an air filter, a connecting conduit, a luer connector inlet, an inlet one-way valve, a fat inlet filtering chamber, a fat outlet filtering chamber, an outlet one-way valve, a luer connector outlet, a three-way switching valve, an inlet filtering cover, a fat outlet and an inlet treatment ball;
the fat treatment container unit comprises: a fat preparation treatment chamber, a treatment ball and a lower annular hole;
the fixing unit includes: positioning fixing pins, positioning clamping grooves, a filter cover pressing plate and a sealing ring;
the air filter is connected with the fat inlet filtering chamber through a connecting conduit; the luer connector inlet is connected with the fat inlet filtering chamber through an inlet one-way valve; an inlet treatment ball, a filter cover pressing plate and an inlet filter cover are arranged in the fat inlet filter chamber, meanwhile, one end of the fat inlet filter chamber is connected with the fat preparation treatment chamber, and a sealing ring is arranged at the interface; the fat preparation treatment chamber is internally provided with a treatment ball, the outer wall of the fat preparation treatment chamber is provided with a positioning fixing pin and a positioning groove, and the tail end of the fat preparation treatment chamber is provided with a lower annular hole; meanwhile, the other end of the fat preparation treatment chamber is provided with a fat outlet filtering chamber; an outlet one-way valve is arranged on the fat outlet filtering chamber and is connected with a luer connector outlet; the luer connector outlet is connected with the fat outlet, and meanwhile, the fat outlet is provided with a three-way change valve.
The manual fat core cluster cell and fat matrix blood vessel component collector has the beneficial effects that:
(1) By filtering unused connective tissue, homogenizing and purifying adipose tissue, high-quality autologous adipose tissue, clustered cells and adipose-derived stromal vascular fraction rich in various growth factors and adipose-derived stem cells are obtained, immune rejection is reduced, and the therapeutic effects of cosmetology, plastic, aging resistance and regenerative medicine are improved, and the safety, effectiveness and durability of the effect of clinical treatment are improved.
(2) The method is simple to operate, good in applicability, safe and reliable, and provides a novel and convenient method for collecting autologous adipose tissue core colony cells and adipose matrix vascular components in the biomedical engineering field.
Drawings
The invention is described in further detail below with reference to the accompanying drawings.
FIG. 1 is a schematic diagram of a manual adipose core-cluster cell and adipose matrix vascular component harvester according to an embodiment of the invention;
FIG. 2 is a schematic cross-sectional view of a manual adipose core-cluster-cell adipose-derived vascular component harvester according to an embodiment of the present invention;
in the figure:
1. an air filter; 2. a connecting conduit; 3. a luer access; 4. an inlet check valve; 5. a fat inlet filter chamber; 6. a fat preparation processor; 7. positioning a fixing pin; 8. positioning clamping grooves; 9. a fat outlet filter chamber; 10. an outlet check valve; 11. a luer outlet; 12. a three-way switching valve;
14. an inlet filter housing; 15. a filter cover press plate; 16. a seal ring; 17. treating the ball; 18. a lower annular hole; 19. a paper mill outlet; 20. inlet treatment balls.
Detailed Description
1-2, the manual fat core cluster cell and fat matrix blood vessel component collector comprises a fat cleaning and filtering unit, a fat treatment container unit and a fixing unit;
the fat cleaning and filtering unit comprises an air filter 1, a connecting conduit 2, a luer connector inlet 3, an inlet one-way valve 4, a fat inlet filtering chamber 5, a fat outlet filtering chamber 9, an outlet one-way valve 10, a luer connector outlet 11, a three-way switching valve 12, an inlet filtering cover 14, a fat outlet 19 and an inlet treatment ball 20;
the fat treatment container unit comprises: a fat preparation treatment chamber 6, a treatment ball 17, a lower annular hole 18;
the fixing unit includes: a positioning fixing pin 7, a positioning clamping groove 8, a filter cover pressing plate 15 and a sealing ring 16;
the air filter 1 is connected with a fat inlet filtering chamber 5 through a connecting conduit 2; the luer connector inlet 3 is connected with a fat inlet filtering chamber 5 through an inlet one-way valve 4; an inlet treatment ball 20, a filter cover pressing plate 15 and an inlet filter cover 14 are arranged in the fat inlet filter chamber 5, meanwhile, one end of the fat inlet filter chamber 5 is connected with the fat preparation treatment chamber 6, and a sealing ring 16 is arranged at the interface; the fat preparation treatment chamber 6 is internally provided with a treatment ball 17, the outer wall of the treatment ball is provided with a positioning fixing pin 7 and a positioning groove 8, and the tail end of the treatment ball is provided with a lower annular hole 18; meanwhile, the other end of the fat preparation treatment chamber 6 is provided with a fat outlet filtering chamber 9; an outlet one-way valve 10 is arranged on the fat outlet filtering chamber 9, and the outlet one-way valve 10 is connected with a luer connector outlet 11; the luer connector outlet 11 is connected with a fat outlet 19, and meanwhile, a three-way change-over valve 12 is arranged on the fat outlet 19;
in the fat washing and filtering unit, fat taken out of a human body can be injected into the fat inlet filtering chamber 5 through the luer connector inlet 3 and the inlet one-way valve 4, and impurities in the fat can be filtered out by the inlet filtering cover 14 in the fat inlet filtering chamber 5. Then, the treated fat is washed by the washing liquid, the outlet check valve 10 is opened, and the waste liquid flows out through the luer outlet 11 and the three-way change-over valve 12. Finally, the three-way switching valve 12 is adjusted so that the treated purified homogenized fat is withdrawn from the fat outlet 19. The air filter 1 is connected with the fat inlet filtering chamber 5 through the connecting conduit 2, so that normal air exchange between the inside and the outside is ensured, and the fat is in a normal pH value environment. The inlet treatment ball 20 in the fat inlet filtering chamber 5 can perform initial treatment on fat, so that the contents of core cluster cells and fat matrix vascular components are greatly obtained;
the fat treatment container unit is mainly responsible for the crushing and homogenizing treatment of human body fat. The processing balls 17 are arranged in the fat preparation processing chamber 6, after fat is injected from the filtering unit, the assembly is rapidly and reciprocally shaken up and down for a period of time, so that the processing balls 17 in the fat preparation processing chamber 6 and the fat vibrate up and down together to collide with the processing balls 17 to achieve the effect of crushing and homogenizing the fat in the fat preparation processing chamber 6. Meanwhile, the lower annular hole 18 not only prevents the processing ball 17 from rushing out of the fat preparation processing chamber 6, but also can homogenize the fat by striking the annular hole;
the fixing unit is responsible for fixing the components. The positioning fixing pin 7 and the positioning clamping groove 8 are used for fixing the whole equipment; the filter housing press 15 is used to secure the inlet filter housing 14 of the filter unit. The sealing ring 16 ensures tightness of the fat handling unit.
The manual fat core cluster cells and fat matrix blood vessel component collector comprises the following operation steps:
(1) Opening the inlet check valve and the outlet check valve, injecting cleaning liquid into the fat preparation treatment chamber and discharging;
(2) Closing the outlet one-way valve, and injecting human body fat into the fat preparation treatment chamber through the filtering unit;
(3) Closing the inlet one-way valve, manually processing the loop component to reciprocate up and down to shake for a period of time, newly opening the inlet one-way valve and the outlet one-way valve, adjusting the three-way switching valve, injecting cleaning liquid, discharging waste liquid, and closing the outlet one-way valve;
(4) And 3, repeating the step 3 for a plurality of times, and finally, adjusting the three-way switching valve again, and extracting the purified and homogenized fat from the fat outlet for clinical application.
The embodiments have been described so as to facilitate a person of ordinary skill in the art in order to understand and apply the present technology, it will be apparent to those skilled in the art that various modifications may be made to these examples and that the general principles described herein may be applied to other embodiments without undue burden. Accordingly, the present application is not limited to the above embodiments, and those skilled in the art, based on the disclosure of the present application, should make improvements and modifications within the scope of the present application.
Claims (1)
1. A method for manually collecting fat core cluster cells and fat matrix vascular components, which is characterized by comprising the following steps of:
the method is carried out by using a manual fat core cluster cell and fat matrix vascular component collector;
the manual fat core cluster cells and fat matrix blood vessel component collector comprises a fat cleaning and filtering unit, a fat treatment container unit and a fixing unit; the fat cleaning and filtering unit comprises an air filter (1), a connecting conduit (2), a luer connector inlet (3), an inlet one-way valve (4), a fat inlet filtering chamber (5), a fat outlet filtering chamber (9), an outlet one-way valve (10), a luer connector outlet (11), a three-way switching valve (12), an inlet filtering cover (14), a fat outlet (19) and an inlet treatment ball (20); the fat treatment container unit comprises a fat preparation treatment chamber (6), a treatment ball (17) and a lower annular hole (18); the fixing unit comprises a positioning fixing pin (7), a positioning clamping groove (8), a filter cover pressing plate (15) and a sealing ring (16); the air filter (1) is connected with the fat inlet filtering chamber (5) through a connecting conduit (2), and the luer connector inlet (3) is connected with the fat inlet filtering chamber (5) through an inlet one-way valve (4); an inlet treatment ball (20), a filter cover pressing plate (15) and an inlet filter cover (14) are arranged in the fat inlet filter chamber (5), the filter cover pressing plate (15) is used for fixing the inlet filter cover (14), and the inlet filter cover (14) is used for filtering out impurities in fat; one end of the fat inlet filtering chamber (5) is connected with the fat preparation processing chamber (6), and a sealing ring (16) is arranged at the interface; the fat preparation treatment chamber (6) is internally provided with a treatment ball (17), the outer wall is provided with a positioning fixing pin (7) and a positioning clamping groove (8), the tail end is provided with a lower annular hole (18), the positioning fixing pin (7) and the positioning clamping groove (8) are used for fixing the whole collector, and the lower annular hole (18) is used for preventing the treatment ball (17) from rushing out of the fat preparation treatment chamber (6) and promoting fat homogenization; the other end of the fat preparation treatment chamber (6) is provided with a fat outlet filtering chamber (9), the fat outlet filtering chamber (9) is provided with an outlet one-way valve (10), the outlet one-way valve (10) is connected with a luer connector outlet (11), the luer connector outlet (11) is connected with a fat outlet (19), and the fat outlet (19) is provided with a three-way change-over valve (12);
the method comprises the following operation steps:
s1, opening the inlet check valve (4) and the outlet check valve (10), and injecting cleaning liquid into the fat preparation treatment chamber (6) and discharging;
s2, closing the outlet one-way valve (10), and injecting fat taken out of a human body into the fat preparation treatment chamber (6) through the luer connector inlet (3) and the fat inlet filtering chamber (5), wherein the inlet treatment ball (20) performs initial treatment on the fat in the fat inlet filtering chamber (5);
s3, closing the inlet one-way valve (4), manually and rapidly shaking the collector up and down in a reciprocating manner for a period of time, enabling a treatment ball (17) in the fat preparation treatment chamber (6) to vibrate up and down together with fat, then re-opening the inlet one-way valve (4) and the outlet one-way valve (10), adjusting the three-way switching valve (12), injecting cleaning liquid to clean the treated fat, discharging waste liquid, and closing the outlet one-way valve (10);
s4, repeating the step S3 for a plurality of times, finally adjusting the three-way switching valve (12), and extracting the purified and homogenized fat from the fat outlet (19) for clinical application.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611022907.3A CN106367320B (en) | 2016-11-21 | 2016-11-21 | Manual fat core cluster cell and fat matrix blood vessel component collector |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611022907.3A CN106367320B (en) | 2016-11-21 | 2016-11-21 | Manual fat core cluster cell and fat matrix blood vessel component collector |
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| CN106367320A CN106367320A (en) | 2017-02-01 |
| CN106367320B true CN106367320B (en) | 2023-11-14 |
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| CN108410711A (en) * | 2017-11-09 | 2018-08-17 | 上海美致臻生物医学科技有限公司 | A kind of full-automatic fatty core clustered cells, lipid substrate vascular component Acquisition Instrument |
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| US9296781B2 (en) * | 2011-06-28 | 2016-03-29 | Veris Medical, Inc. | System and method for collagen isolation |
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