CN108056063A - A kind of cultural method of layer of green-shell egg - Google Patents

A kind of cultural method of layer of green-shell egg Download PDF

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CN108056063A
CN108056063A CN201711344038.0A CN201711344038A CN108056063A CN 108056063 A CN108056063 A CN 108056063A CN 201711344038 A CN201711344038 A CN 201711344038A CN 108056063 A CN108056063 A CN 108056063A
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extract
green
layer
carries out
extraction
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李恪言
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Liuzhou Elephant Line Education Science And Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/02—Breeding vertebrates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/26—Compounds containing phosphorus
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The present invention relates to cultural technique field more particularly to a kind of cultural methods of layer of green-shell egg, operate in accordance with the following steps：For the layer of green-shell egg in 23 week old, the feed obtained using configuration is fed, 3 times/day, and 100 300g/ every time, and by its free choice feeding to all you can eat；The composition of the feed is as follows：Corn flour 50 60%, oyster shell whiting 10 15%, dregs of beans 8 15%, fermentation eggshell material 5 8%, fish meal 2 5%, salt 1 3%, calcium monohydrogen phosphate 3 5%, plant extracts mixture 2 5%.Advantage of the present invention：Growth rate can not only be improved by largely studying the feed for finding to feed the application in periodic time, a series of intercurrent disease caused by also control blood fat raises well, and feed has preferable insecticidal action, ensure that layer of green-shell egg free choice feeding eats harmful substance intoxicating phenomenon by mistake, so as to improve the survival rate of layer of green-shell egg, and then increase the purpose of income.

Description

A kind of cultural method of layer of green-shell egg
Technical field
The present invention relates to cultural technique field more particularly to a kind of cultural methods of layer of green-shell egg.
Background technology
Layer of green-shell egg is gained the name when producing green-egg-shelled, it is characterized in that the color of shell laid eggs is in green, it is the peculiar fowl of China Kind, it is classified as " whole nation special type protection of resources project " by the Ministry of Agriculture.The chicken kind premunition is strong, wide adaptability, eating green grass dish leaf, raises Support management, epidemic prevention go out disease and commonly family chicken be not different.
The layer of green-shell egg bodily form is smaller, solid compact, and Quick off the mark, well-balanced beautiful, sexal maturity is more early, and egg production is higher.Into 3.2~4.5 jin, a traditional unit of weight of year cock weight, 1.9~3.1 jin, a traditional unit of weight of hen weight is laid eggs 160~180 pieces in year.The chicken kind has apparent high The ability of environmental change is resisted in common family chicken, north and south can cultivate.
The content of the invention
The goal of the invention of the present invention is：A kind of cultural method of layer of green-shell egg is provided, is found by largely studying The feed that periodic time feeds the application can not only improve growth rate, also have a system caused by the rise of control blood fat well The intercurrent disease of row, and feed has preferable insecticidal action, ensures that layer of green-shell egg free choice feeding eats harmful substance intoxicating phenomenon by mistake, So as to improve the survival rate of layer of green-shell egg, and then increase the purpose of income.
In order to realize foregoing invention purpose, the technical solution adopted by the present invention is as follows：
A kind of cultural method of layer of green-shell egg, operates in accordance with the following steps：
For the layer of green-shell egg in 2-3 week old, the feed configured according to the raw material of following mass percentage carries out Feeding, 3 times/day, and 100-300g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Corn flour 45-55%, plant extracts mixture 5-10%, oyster shell whiting 8-15%, Dregs of beans 10-15%, fermentation eggshell material 5-10%, fish meal 3-5%, salt 1-3%, calcium monohydrogen phosphate 2-4%；
The fermentation eggshell material is after discarded eggshell is crushed, and adds in mother-in-law U.S. degree to ferment after the brown sugar water of 30-45 10-20 days；
The plant extracts mixture is made according to percent by weight of following raw material：Folum Ilicis extract 15- 20%th, Chinese wistaria extract 8-15%, ophiopogon japonicus extract 10-18%, Stenoloma chusana extract 10-15%, sorosis extract 4-9%, chrysanthemum Lettuce extract 5-13%, cranberry extract 7-12%, Herb Gynostemmae Pentaphylli extract 7-12%, Cacumen Platycladi extract 3-10%, Uricularia polytricha Extract 3-5%；
The preparation method of the plant extracts mixture is：After above-mentioned extract is mixed according to percent by weight After the 10-30% water of addition total weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added in：Hydroxylmethyl cellulose Element：Dope is 0.15-0.23 according to ratio of weight and number:0.55-0.65:After 10-12 is stirred evenly, it is put into drying machine and is done It is dry, the use of power is 50-80W while drying, frequency is that 30-50 hertz of ultrasonic waves are modified processing, you can obtain powdery Plant extracts mixture.
It further illustrates, the Folum Ilicis extract carries out infusion after fresh Ilex Latifolia Thunb to be added in the water of 5-10 times of volume 30-40 minutes, be filtered while hot, take filtrate, by filtrate carry out stop heating after heating is concentrated into 1.02 times of original volume, Obtain medicinal extract be subsequently added into cellulase, 70-80% (v/v) ethanol solution carry out surname extraction 1-2 it is small when, by filtrate after filtering Be dried under reduced pressure, crush after can obtain Folum Ilicis extract, 1.35-2.64mg/g containing amyrin, lupeol 1.02- 2.64mg/g, cupreol 2.63-5.24mg/g, amaroid saponin(e 1.25-2.64mg/g；The medicinal extract：Cellulase：Ethyl alcohol The weight ratio of solution is 5-10:1-2:21-31.
It further illustrates, after the Chinese wistaria extract crushes 1kg Chinese wistaria seeds, puts into supercritical CO2Extraction dress In putting, when extraction 3-4 is small, extracting solution is collected, filter residue is added in 70-80% (v/v) ethanol solution of 3-5 times of filter residue quality, into Row refluxing extraction 20-30 minutes, the temperature of reflux is at 65-85 DEG C, then pours into 0.3 μm of ceramics with the flow velocity of 0.1-0.2m/s Membrane module carries out micro-filtration, under 0.1-0.2MPa pressure differentials, successively with the membrane module of molecular cut off 10000Da, 5000Da, with Cross-current flow carries out loop ultrafiltration processing, the Chinese wistaria extract that molecular weight ranges are 5000Da-6000Da is obtained, containing diacetone alcohol 0.36-0.56mg/g, cupreol 0.48-1.12mg/g, β-daucosterol 0.15-0.23mg/g, asteriasterol 0.11- 0.24mg/g。
It further illustrates, the ophiopogon japonicus extract is the 60- that Radix Ophiopogonis is added in 3-5 times of powder weight after pulverizing 80% (v/v) ethanol solution carries out refluxing extraction 3 times, and 20 minutes every time, water bath method in evaporating dish is placed after filtering, then into Row, which is dried under reduced pressure, to 1.01 times of original volume, to be added in 70-80% (v/v) ethanol solution containing 1-2% acetic acid solutions and is determined Rong Hou, the speed using flow velocity as 0.1-0.2m/s carry out the miillpore filter that aperture is 0.5-0.8 μm and are filtered, you can obtain wheat Winter extract, 2.02-4.36mg/g containing ophiopogonin.
Further illustrate, the Stenoloma chusana extract by fresh Stenoloma chusana herb clean after add in 3-5 times of volume distilled water into Row homogeneous is beaten, be subsequently added into solvent extract 1-3 it is small when, it is 1000-2000Da's then to carry out micro-filtration molecular cut off Trapped fluid is carried out separation 3-5 minutes in rotating speed is the seperator of 2000-3000r/min, supernatant is taken to spray by active material Stenoloma chusana extract is can obtain after drying, cupreol 1.62mg/g-4.32mg/g, glucosides are contained in obtained substance 1.58mg/g-4.30mg/g。
It further illustrates, the water that sorosis herb is added in 2-3 times of volume by the sorosis extract stirs in mixer Mix slurry, be subsequently added into after 3-5 times of water stirs evenly carry out water-bath extract 30-60 minutes, thick liquid is obtained by filtration, by thick liquid with The flow velocity of 0.1-0.2m/s pours into polyamide chromatography column and often chromatographs, and 3 elutions are carried out using 70-80% (v/v) ethanol solution, It is 60-70 DEG C that the pressure of elution, which is followed successively by -0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution, is then filtered, decompression is dense Sorosis extract, 1.13-2.35mg/g containing tannic acid, anthocyanidin 2.31-3.24mg/g are obtained after contracting, dry, crushing.
It further illustrates, the chicory extract adds in 5-10 times of volume water progress water-bath after the flower of witloof is smashed to pieces carries It takes 20-30 minutes, the microwave that extraction process frequency of use is 200-300 hertz carries out assisted extraction, after filtering depressurize dense It being reduced to 1.02 times of original volume, adds in 20-30% (v/v) methanol solution and carry out 3 elutions, the pressure of elution is followed successively by- 0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution for 60-70 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain Chicory extract, 5.10-8.25mg/g containing alkaloid.
It further illustrates, blueberry is put into mixer and is stirred crushing by the cranberry extract, then using 65-75% (v/v) ethanol solution progress alcohol extracting at temperature is -5--3 DEG C is stayed overnight, and centrifugation of the rotating speed for 300-400r/min is used after overnight Machine, which is centrifuged, is obtained by filtration thick liquid, when room temperature extraction 1-3 is small after thick liquid is mixed with protease, blueberry is obtained by filtration and carries Object is taken, 0.69-2.15mg/g containing anthocyanin, ursin 0.69-1.36mg/g；
The water that gynostemma pentaphylla is added in 2-5 times of volume by the Herb Gynostemmae Pentaphylli extract is smashed to pieces, and electric field strength is used after smashing into slurry to pieces It is handled for the high-pressure pulse electric of 25KV/cm, the time of pulse is 5 × 10-6-10×10-6S, the frequency of pulse are conspicuous for 30-50 Hereby, 5-10 times 70-80% (v/v) ethanol solution is added in after being handled and carries out ultrasonic extraction 10-20 minutes using Ultrasound Instrument, The current strength of Ultrasound Instrument be 5A/25W-10A/25W, then with the flow velocity of 0.1-0.25m/s pour into 0.5 μm of ceramic film component into Row micro-filtration, under 0.1-0.2MPa pressure differentials, successively with the membrane module of molecular cut off 50000Da, 1O000Da, with cross-flow side Formula carries out loop ultrafiltration processing, obtains the Herb Gynostemmae Pentaphylli extract that molecular weight ranges are 10000Da-15000Da, 2.36- containing saponin(e 8.36mg/g, flavonoids 2.36-2.95mg/g.
It further illustrates, the Cacumen Platycladi extract obtains adding in 70-80% (v/v) ethyl alcohol after cacumen biotae is crushed Solution carries out Xun Huan extraction and obtains extracting solution, and it is 2.0-3.0 that extracting solution is added in salt acid for adjusting pH, and then water-bath adds while stirring Heat continues stirring 5-10 minutes until occurring filiform in extracting solution and starting timing, filters to take filtrate in time, and filter residue repeats above-mentioned Extraction step 2 times can obtain Cacumen Platycladi extract after the filtrate merging of 3 times is dried under reduced pressure, is crushed, contain flavones 0.69-2.01mg/g, terpene substances 0.74-1.96mg/g, tannin 0.65-1.25mg/g；
After Uricularia polytricha is dried the Uricularia polytricha extract, 70-80% (v/v) ethyl alcohol for adding in 5-10 times of volume is molten Liquid filters to take the 0.01-0.02%'s of filtrate addition filtrate gross mass using when progress surname extraction 2-3 is small after apparatus,Soxhlet's Stirring after chitosan solution filters off filtrate and carries out heating concentration, be freeze-dried to obtain Uricularia polytricha and carry until filtrate clarification Object is taken, 115.26-157.36mg/g containing Polysaccharide from Auricularia Polytricha Sacc.
It further illustrates, the sweetener is xylitol or oligoisomaltose.
In conclusion by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
Corn flour of the present invention, oyster shell whiting, dregs of beans, fermentation eggshell material, fish meal, salt, calcium monohydrogen phosphate, plant extracts mixing Material has reached after proportioning containing organic matter 58.12-69.75%, trace element 5.96-8.32%, effective carbon 35.65- 38.32%th, effective calcium 15.63-14.65% can meet layer of green-shell egg nutrient elements required for the growth, promote growth, and added Add in plant extracts mixture rich in substantial amounts of Flavonoid substances, alkaloid, benzine soap glycoside substance, tannin class object The phenomenon that matter, collective effect plays desinsection, antimicrobial flora is grown, is so that layer of green-shell egg has after free choice feeding other plant There is better resistivity, and increase the amount of exercise of layer of green-shell egg by free choice feeding, building up health reduces sick rate, appropriate to transport Dynamic auxiliary reduces grease and accumulates so that delicious meat and non-greasy, and the plant extracts mixture added has drop blood well The effect of fat effect improves the survival rate of layer of green-shell egg.
The effect of each plant extracts mixture：Ilex Latifolia Thunb, blade leathery.It is complete and straight, in oblong, leaf margin Have and dredge sharp sawtooth such as thorn-like, blade face is smooth, bluish yellow color or cinerous；Blade back color is slightly shallow, and master pulse, net vein are apparent.It is cold in nature, taste It is bitter, sweet.Return liver warp, lung channel, stomach.Wind and heat dispersing, relieving restlessness cough-relieving eliminate indigestion and phlegm.Chinese wistaria, sweet in flavor, bitter, slightly warm in nature, mild toxicity.Return Kidney channel.For Shui Yin diseases, edema, arthralgia, intestinal parasitic disease.Field pennycress, cool in nature, acrid flavour, hardship.Return stomach, large intestine channel, liver Through.Clearing heat and detoxicating, removing blood stasis and expelling pus.Sorosis is cold in nature, sweet in flavor, sour.Thoughts of returning home warp, Liver Channel, kidney channel.It enriches blood enriching yin, fluid dryness.Chrysanthemum Lettuce, it is cool in nature, it is mildly bitter flavor, salty.Clearing liver cholagogic, stomach strengthening and digestion promoting, inducing diuresis to remove edema.Blueberry, sour, sweet and neutral.For stop dysentery.For intestines Inflammation, dysentery.The effect of cacumen biotae, cooling blood and hemostasis, black beard and hair, cough-relieving asthma.Uricularia polytricha, property is sweet, and taste is put down, and lung, kidney, liver three pass through.It mends Qi and blood, moistening lung, hemostasis.For Qi and blood deficiency, four limbs are twitched, cough due to deficiency of the lung, are spat blood, and are spitted blood, bleeding from five sense organs or subcutaneous tissue, uterine bleeding, high blood pressure, just It is secret.Radix Ophiopogonis, cold nature, sweet in flavor, slight bitter.Thoughts of returning home warp, lung channel, stomach.With controlling dryness of the lung dry cough, haematemesis, lung carbuncle, consumptive disease dysphoria with smothery sensation, disappear Yearningly, pyreticosis Tianjin wound, dry throat, constipation etc..Stenoloma chusana slight bitter is trembled with fear；It relieves inflammation or internal heat, brings down a fever, dampness removing, hemostasis.10 Plant Extracts mix It closes material to complement each other, cholesterol level can be reduced, so as to prevent a variety of syndromes as caused by cholesterol, play good drop The effect of low layer of green-shell egg sick rate.
Specific embodiment
All features or disclosed all methods disclosed in this specification or in the process the step of, except mutually exclusive Feature and/or step beyond, can combine in any way.This specification (including any accessory claim, summary) Disclosed in any feature, unless specifically stated, can be replaced by other alternative features that are equivalent or have similar purpose. I.e., unless specifically stated, each feature is an example in a series of equivalent or similar characteristics.
Embodiment 1
A kind of cultural method of layer of green-shell egg, operates in accordance with the following steps：
For the layer of green-shell egg in 2 week old, the feed configured according to the raw material of following mass percentage is raised Hello, 3 times/day, and 100g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：The composition of the feed is as follows：Corn flour 45%, plant extracts mixture 10%th, oyster shell whiting 8%, dregs of beans 15%, fermentation eggshell material 10%, fish meal 5%, salt 3%, calcium monohydrogen phosphate 4%；
The plant extracts mixture is made according to percent by weight of following raw material：Folum Ilicis extract 15%, Chinese wistaria extract 15%, ophiopogon japonicus extract 18%, Stenoloma chusana extract 15%, sorosis extract 4%, chicory extract 6%, blueberry Extract 7%, Herb Gynostemmae Pentaphylli extract 7%, Cacumen Platycladi extract 10%, Uricularia polytricha extract 3%；
The fermentation eggshell material is after discarded eggshell is crushed, and adds in mother-in-law U.S. degree to ferment 10 days after 30 brown sugar water ；
The preparation method of the plant extracts mixture is：After above-mentioned extract is mixed according to percent by weight After 10% water of addition total weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added in：Hydroxymethyl cellulose： Dope is 0.15 according to ratio of weight and number:0.55:It after 10 stir evenly, is put into drying machine and is dried, drying while makes It is 50W with power, frequency is that 30 hertz of ultrasonic waves are modified processing, you can obtain powdered plant extract mixture.
It further illustrates, the Folum Ilicis extract will carry out infusion 30 after the water of fresh Ilex Latifolia Thunb 5 times of volumes of addition and divide Clock is filtered while hot, takes filtrate, by filtrate carry out stop heating after heating is concentrated into 1.02 times of original volume, soaked Cream is subsequently added into cellulase, 70% (v/v) ethanol solution carry out surname extraction 1 it is small when, it is dry that filtrate is subjected to decompression after filtering Folum Ilicis extract is can obtain after dry, crushing, 1.35mg/g containing amyrin, lupeol 1.02mg/g, cupreol 2.63mg/g, amaroid saponin(e 1.25mg/g；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 5:1:21.
It further illustrates, after the Chinese wistaria extract crushes 1kg Chinese wistaria seeds, puts into supercritical CO2Extraction dress In putting, when extraction 3 is small, extracting solution is collected, filter residue is added in 70% (v/v) ethanol solution of 3 times of filter residue quality, carried out reflux and carry It taking 20 minutes, the temperature of reflux is at 65 DEG C, then pours into 0.3 μm of ceramic film component with the flow velocity of 0.1m/s and carries out micro-filtration, Under 0.12MPa pressure differentials, successively with the membrane module of molecular cut off 10000Da, 5000Da, loop ultrafiltration is carried out with cross-current flow Processing, obtain molecular weight ranges be 5000Da Chinese wistaria extract, 0.36mg/g containing diacetone alcohol, cupreol 0.48mg/g, β-daucosterol 0.15mg/g, asteriasterol 0.11mg/g.
It further illustrates, the ophiopogon japonicus extract is that Radix Ophiopogonis is added in the 60% of 3 times of powder weight after pulverizing (v/v) ethanol solution carries out refluxing extraction 3 times, 20 minutes every time, and water bath method in evaporating dish is placed after filtering, is then subtracted Dry 1.01 times to original volume are pressed dry, are added in after 70% (v/v) ethanol solution containing 1% acetic acid solution carries out constant volume, with stream The speed that speed is 0.11m/s carries out the miillpore filter that aperture is 0.5 μm and is filtered, you can ophiopogon japonicus extract is obtained, containing Radix Ophiopogonis Saponin(e 2.02mg/g.
It further illustrates, the Stenoloma chusana extract adds in 3 times of volumes distilled water after fresh Stenoloma chusana herb is cleaned carries out Homogeneous is beaten, be subsequently added into solvent extract 1 it is small when, then carry out the active material that micro-filtration molecular cut off is 1000Da, Trapped fluid is subjected to separation 3 minutes in rotating speed is the seperator of 2000r/min, supernatant is taken to can obtain crow after being spray-dried Fragrant-flowered garlic extract contains cupreol 1.62mg/g, glucosides 1.58mg/g in obtained substance.
It further illustrates, the water that sorosis herb is added in 2 times of volumes by the sorosis extract is stirred in mixer Into slurry, it is subsequently added into after 3 times of water stir evenly and carries out water-bath extraction 30 minutes, thick liquid is obtained by filtration, by thick liquid with 0.1m/s's Flow velocity pours into polyamide chromatography column and often chromatographs, and carries out 3 elutions using 70% (v/v) ethanol solution, the pressure of elution is successively For -0.1MPa, 0.1MPa, 0.2MPa, elution temperature be 60 DEG C, be then filtered, be concentrated under reduced pressure, dry, crushes after must To sorosis extract, 1.13mg/g containing tannic acid, anthocyanidin 2.31mg/g.
It further illustrates, the chicory extract adds in 5 times of volumes water after the flower of witloof is smashed to pieces carries out water-bath extraction 20 minutes, the microwave that extraction process frequency of use is 200 hertz carried out assisted extraction, carries out being concentrated under reduced pressure into original volume after filtering 1.02 times, add in 20% (v/v) methanol solution and carry out 3 elutions, the pressure of elution be followed successively by -0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution for 60 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain chicory extract, containing biology Alkali 5.10mg/g.
It further illustrates, blueberry is put into mixer and is stirred crushing by the cranberry extract, then using 65% (v/ V) ethanol solution progress alcohol extracting at temperature is -5 DEG C is stayed overnight, and after overnight rotating speed is used to be centrifuged for the centrifuge of 300r/min Separating and filtering obtains thick liquid, when room temperature extraction 1 is small after thick liquid is mixed with protease, cranberry extract is obtained by filtration, containing pattern Glycosides 0.69mg/g, ursin 0.69mg/g；
The water that gynostemma pentaphylla is added in 2 times of volumes by the Herb Gynostemmae Pentaphylli extract is smashed to pieces, is smashed to pieces into after slurry and is using electric field strength The high-pressure pulse electric processing of 25KV/cm, the time of pulse is 5 × 10-6S, the frequency of pulse is 30 hertz, is added after being handled Enter 5 times 70% (v/v) ethanol solution and carry out ultrasonic extraction 10 minutes using Ultrasound Instrument, the current strength of Ultrasound Instrument is 5A/ 25W then pours into 0.5 μm of ceramic film component with the flow velocity of 0.1m/s and carries out micro-filtration, under 0.1MPa pressure differentials, successively with retention The membrane module of molecular weight 50000Da, 1O000Da carry out loop ultrafiltration processing with cross-current flow, and obtaining molecular weight ranges is The Herb Gynostemmae Pentaphylli extract of 10000Da, 2.36mg/g containing saponin(e, flavonoids 2.36mg/g.
It further illustrates, the Cacumen Platycladi extract obtains addition 70% (v/v) ethyl alcohol after cacumen biotae is crushed molten Liquid carries out cycle extraction and obtains extracting solution, and it is 2.0 that extracting solution is added in salt acid for adjusting pH, then while stirring heating water bath until Occur filiform in extracting solution and start timing continuing stirring 5 minutes, filter to take filtrate in time, filter residue repeats said extracted step 2 It is secondary, Cacumen Platycladi extract is can obtain after the filtrate merging of 3 times is dried under reduced pressure, is crushed, contains flavones 0.69mg/g, terpene Class substance 0.74mg/g, tannin 0.65mg/g；
After Uricularia polytricha is dried the Uricularia polytricha extract, 70% (v/v) ethanol solution for adding in 5 times of volumes uses When progress surname extraction 2 is small after apparatus,Soxhlet's, after 0.01% chitosan solution for filtering to take filtrate addition filtrate gross mass Stirring filters off filtrate and carries out heating concentration until filtrate clarification, is freeze-dried to obtain Uricularia polytricha extract containing Uricularia polytricha Polysaccharide 115.26mg/g.
It further illustrates, the sweetener is xylitol.
Layer of green-shell egg disease rate by above method raising is 1.2%.
Embodiment 2
A kind of cultural method of layer of green-shell egg, operates in accordance with the following steps：
For the layer of green-shell egg in 3 week old, the feed configured according to the raw material of following mass percentage is raised Hello, 3 times/day, and 300g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Corn flour 55%, plant extracts mixture 5%, oyster shell whiting 15%, dregs of beans 10%th, ferment eggshell material 9%, fish meal 3%, salt 1%, calcium monohydrogen phosphate 2%；
The fermentation eggshell material is after discarded eggshell is crushed, and adds in mother-in-law U.S. degree to ferment 20 days after 45 brown sugar water ；
The plant extracts mixture is made according to percent by weight of following raw material：Folum Ilicis extract 20%, Chinese wistaria extract 8%, ophiopogon japonicus extract 10%, Stenoloma chusana extract 10%, sorosis extract 9%, chicory extract 13%, blueberry Extract 12%, Herb Gynostemmae Pentaphylli extract 12%, Cacumen Platycladi extract 3%, Uricularia polytricha extract 5%；
The preparation method of the plant extracts mixture is：After above-mentioned extract is mixed according to percent by weight After 30% water of addition total weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added in：Hydroxymethyl cellulose： Dope is 0.23 according to ratio of weight and number:0.65:It after 12 stir evenly, is put into drying machine and is dried, drying while makes It is 80W with power, frequency is that 50 hertz of ultrasonic waves are modified processing, you can obtain powdered plant extract mixture.
It further illustrates, the Folum Ilicis extract will carry out infusion 40 after the water of fresh Ilex Latifolia Thunb 10 times of volumes of addition and divide Clock is filtered while hot, takes filtrate, by filtrate carry out stop heating after heating is concentrated into 1.02 times of original volume, soaked Cream is subsequently added into cellulase, 80% (v/v) ethanol solution carry out surname extraction 2 it is small when, it is dry that filtrate is subjected to decompression after filtering Folum Ilicis extract is can obtain after dry, crushing, 2.64mg/g containing amyrin, lupeol 2.64mg/g, cupreol 5.24mg/g, amaroid saponin(e 2.64mg/g；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 10:2:31.
It further illustrates, after the Chinese wistaria extract crushes 1kg Chinese wistaria seeds, puts into supercritical CO2Extraction dress In putting, when extraction 4 is small, extracting solution is collected, filter residue is added in 80% (v/v) ethanol solution of 5 times of filter residue quality, carried out reflux and carry It taking 30 minutes, the temperature of reflux is at 85 DEG C, then pours into 0.3 μm of ceramic film component with the flow velocity of 0.2m/s and carries out micro-filtration, Under 0.1MPa pressure differentials, successively with the membrane module of molecular cut off 10000Da, 5000Da, loop ultrafiltration is carried out with cross-current flow Processing, obtain molecular weight ranges be 6000Da Chinese wistaria extract, 0.56mg/g containing diacetone alcohol, cupreol 1.12mg/g, β-daucosterol 0.23mg/g, asteriasterol 0.24mg/g.
It further illustrates, the ophiopogon japonicus extract is that Radix Ophiopogonis is added in the 80% of 5 times of powder weight after pulverizing (v/v) ethanol solution carries out refluxing extraction 3 times, 20 minutes every time, and water bath method in evaporating dish is placed after filtering, is then subtracted Dry 1.01 times to original volume are pressed dry, are added in after 80% (v/v) ethanol solution containing 2% acetic acid solution carries out constant volume, with stream The speed that speed is 0.2m/s carries out the miillpore filter that aperture is 0.8 μm and is filtered, you can obtains ophiopogon japonicus extract, soap containing Radix Ophiopogonis Glycosides 4.36mg/g.
It further illustrates, the Stenoloma chusana extract adds in 5 times of volumes distilled water after fresh Stenoloma chusana herb is cleaned carries out Homogeneous is beaten, be subsequently added into solvent extract 3 it is small when, then carry out the active material that micro-filtration molecular cut off is 2000Da, Trapped fluid is subjected to separation 5 minutes in rotating speed is the seperator of 3000r/min, supernatant is taken to can obtain crow after being spray-dried Fragrant-flowered garlic extract contains cupreol 4.32mg/g, glucosides 4.30mg/g in obtained substance.
It further illustrates, the water that sorosis herb is added in 3 times of volumes by the sorosis extract is stirred in mixer Into slurry, it is subsequently added into after 5 times of water stir evenly and carries out water-bath extraction 60 minutes, thick liquid is obtained by filtration, by thick liquid with 0.2m/s's Flow velocity pours into polyamide chromatography column and often chromatographs, and carries out 3 elutions using 80% (v/v) ethanol solution, the pressure of elution is successively For -0.1MPa, 0.1MPa, 0.2MPa, elution temperature be 70 DEG C, be then filtered, be concentrated under reduced pressure, dry, crushes after must To sorosis extract, 2.35mg/g containing tannic acid, anthocyanidin 3.24mg/g.
It further illustrates, the chicory extract adds in 10 times of volumes water after the flower of witloof is smashed to pieces carries out water-bath extraction 30 minutes, the microwave that extraction process frequency of use is 300 hertz carried out assisted extraction, carries out being concentrated under reduced pressure into original volume after filtering 1.02 times, add in 30% (v/v) methanol solution and carry out 3 elutions, the pressure of elution be followed successively by -0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution for 70 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain chicory extract, containing biology Alkali 8.25mg/g.
It further illustrates, blueberry is put into mixer and is stirred crushing by the cranberry extract, then using 75% (v/ V) ethanol solution progress alcohol extracting at temperature is -3 DEG C is stayed overnight, and after overnight rotating speed is used to be centrifuged for the centrifuge of 400r/min Separating and filtering obtains thick liquid, when room temperature extraction 3 is small after thick liquid is mixed with protease, cranberry extract is obtained by filtration, containing pattern Glycosides 2.15mg/g, ursin 1.36mg/g；
The water that gynostemma pentaphylla is added in 5 times of volumes by the Herb Gynostemmae Pentaphylli extract is smashed to pieces, is smashed to pieces into after slurry and is using electric field strength The high-pressure pulse electric processing of 25KV/cm, the time of pulse is 10 × 10-6S, the frequency of pulse is 50 hertz, after being handled It adds in 10 times 80% (v/v) ethanol solution and carries out ultrasonic extraction 20 minutes using Ultrasound Instrument, the current strength of Ultrasound Instrument is 10A/25W then pours into 0.5 μm of ceramic film component with the flow velocity of 0.25m/s and carries out micro-filtration, under 0.2MPa pressure differentials, successively With the membrane module of molecular cut off 50000Da, 1O000Da, loop ultrafiltration processing is carried out with cross-current flow, obtains molecular weight ranges For the Herb Gynostemmae Pentaphylli extract of 15000Da, 8.36mg/g containing saponin(e, flavonoids 2.95mg/g.
It further illustrates, the Cacumen Platycladi extract obtains addition 80% (v/v) ethyl alcohol after cacumen biotae is crushed molten Liquid carries out cycle extraction and obtains extracting solution, and it is 3.0 that extracting solution is added in salt acid for adjusting pH, then while stirring heating water bath until Occur filiform in extracting solution and start timing continuing stirring 10 minutes, filter to take filtrate in time, filter residue repeats said extracted step 2 It is secondary, Cacumen Platycladi extract is can obtain after the filtrate merging of 3 times is dried under reduced pressure, is crushed, contains flavones 2.01mg/g, terpene Class substance 1.96mg/g, tannin 1.25mg/g；
After Uricularia polytricha is dried the Uricularia polytricha extract, adding in 80% (v/v) ethanol solution of 10 times of volumes makes With carried out after apparatus,Soxhlet's surname extraction 3 it is small when, filter to take filtrate add in filtrate gross mass 0.02% chitosan solution Stirring is until filtrate clarification, filters off filtrate and carry out heating concentration, be freeze-dried to obtain Uricularia polytricha extract, containing hair wood afterwards Fungus polysaccharides 157.36mg/g.
It further illustrates, the sweetener is oligoisomaltose.
Layer of green-shell egg disease rate by above method raising is 1.6%.
Embodiment 3
A kind of cultural method of layer of green-shell egg, operates in accordance with the following steps：
For the layer of green-shell egg in 3 week old, the feed configured according to the raw material of following mass percentage is raised Hello, 3 times/day, and 200g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Corn flour 52%, plant extracts mixture 9%, oyster shell whiting 12%, dregs of beans 13%th, ferment eggshell material 5%, fish meal 4%, salt 2%, calcium monohydrogen phosphate 3%；
The fermentation eggshell material is after discarded eggshell is crushed, and adds in mother-in-law U.S. degree to ferment 15 days after 35 brown sugar water ；
The plant extracts mixture is made according to percent by weight of following raw material：Folum Ilicis extract 17%, Chinese wistaria extract 12%, ophiopogon japonicus extract 13%, Stenoloma chusana extract 12%, sorosis extract 6%, chicory extract 10%, more Tangerine extract 10%, Herb Gynostemmae Pentaphylli extract 10%, Cacumen Platycladi extract 6%, Uricularia polytricha extract 4%；
The preparation method of the plant extracts mixture is：After above-mentioned extract is mixed according to percent by weight After 20% water of addition total weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added in：Hydroxymethyl cellulose： Dope is 0.2 according to ratio of weight and number:0.6:It after 11 stir evenly, is put into drying machine and is dried, drying while uses Power is 70W, frequency is that 40 hertz of ultrasonic waves are modified processing, you can obtains powdered plant extract mixture.
It further illustrates, the Folum Ilicis extract will carry out infusion 35 after the water of fresh Ilex Latifolia Thunb 7 times of volumes of addition and divide Clock is filtered while hot, takes filtrate, by filtrate carry out stop heating after heating is concentrated into 1.02 times of original volume, soaked Cream be subsequently added into cellulase, 75% (v/v) ethanol solution carry out surname extraction 1.2 it is small when, filtrate is depressurized after filtering Folum Ilicis extract is can obtain after dry, crushing, 1.96mg/g containing amyrin, lupeol 1.97mg/g, cupreol 4.02mg/g, amaroid saponin(e 2.04mg/g；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 7:1:15.
It further illustrates, after the Chinese wistaria extract crushes 1kg Chinese wistaria seeds, puts into supercritical CO2Extraction dress In putting, when extraction 3.5 is small, extracting solution is collected, filter residue is added in 75% (v/v) ethanol solution of 4 times of filter residue quality, is flowed back Extraction 26 minutes, the temperature of reflux is at 70 DEG C, then pours into 0.3 μm of ceramic film component with the flow velocity of 0.12m/s and carries out micro-filtration, Under 0.2MPa pressure differentials, successively with the membrane module of molecular cut off 10000Da, 5000Da, it is super that Xun Huan is carried out with cross-current flow Filter is handled, and obtains the Chinese wistaria extract that molecular weight ranges are 5500Da, 0.42mg/g containing diacetone alcohol, cupreol 1.04mg/ G, β-daucosterol 0.19mg/g, asteriasterol 0.18mg/g.
It further illustrates, the ophiopogon japonicus extract is that Radix Ophiopogonis is added in the 71% of 4 times of powder weight after pulverizing (v/v) ethanol solution carries out refluxing extraction 3 times, 20 minutes every time, and water bath method in evaporating dish is placed after filtering, is then subtracted Dry 1.01 times to original volume are pressed dry, are added in after 75% (v/v) ethanol solution containing 1.2% acetic acid solution carries out constant volume, with The speed that flow velocity is 0.15m/s carries out the miillpore filter that aperture is 0.6 μm and is filtered, you can ophiopogon japonicus extract is obtained, containing wheat Winter saponin(e 3.15mg/g.
It further illustrates, the Stenoloma chusana extract adds in 4 times of volumes distilled water after fresh Stenoloma chusana herb is cleaned carries out Homogeneous is beaten, be subsequently added into solvent extract 2 it is small when, then carry out the active material that micro-filtration molecular cut off is 1500Da, Trapped fluid is subjected to separation 4 minutes in rotating speed is the seperator of 2500r/min, supernatant is taken to can obtain crow after being spray-dried Fragrant-flowered garlic extract contains cupreol 2.35mg/g, glucosides 3.21mg/g in obtained substance.
It further illustrates, the water that sorosis herb is added in 2.5 times of volumes by the sorosis extract stirs in mixer Slurry is mixed, is subsequently added into after 4 times of water stir evenly and carries out water-bath extraction 50 minutes, thick liquid is obtained by filtration, by thick liquid with 0.2m/s Flow velocity pour into polyamide chromatography column and often chromatograph, carry out 3 elutions using 75% (v/v) ethanol solution, the pressure of elution according to Secondary is -0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution are 65 DEG C, is then filtered, after being concentrated under reduced pressure, dry, crushing Obtain sorosis extract, 2.04mg/g containing tannic acid, anthocyanidin 2.82mg/g.
It further illustrates, the chicory extract adds in 7 times of volumes water after the flower of witloof is smashed to pieces carries out water-bath extraction 25 minutes, the microwave that extraction process frequency of use is 250 hertz carried out assisted extraction, carries out being concentrated under reduced pressure into original volume after filtering 1.02 times, add in 25% (v/v) methanol solution and carry out 3 elutions, the pressure of elution be followed successively by -0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution for 65 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain chicory extract, containing biology Alkali 7.12mg/g.
It further illustrates, blueberry is put into mixer and is stirred crushing by the cranberry extract, then using 70% (v/ V) ethanol solution progress alcohol extracting at temperature is -4 DEG C is stayed overnight, and after overnight rotating speed is used to be centrifuged for the centrifuge of 385r/min Separating and filtering obtains thick liquid, when room temperature extraction 2 is small after thick liquid is mixed with protease, cranberry extract is obtained by filtration, containing pattern Glycosides 1.15mg/g, ursin 0.97mg/g；
The water that gynostemma pentaphylla is added in 4 times of volumes by the Herb Gynostemmae Pentaphylli extract is smashed to pieces, is smashed to pieces into after slurry and is using electric field strength The high-pressure pulse electric processing of 25KV/cm, the time of pulse is 7 × 10-6S, the frequency of pulse is 38 hertz, is added after being handled Enter 6 times 75% (v/v) ethanol solution and carry out ultrasonic extraction 17 minutes using Ultrasound Instrument, the current strength of Ultrasound Instrument is 8A/ 25W then pours into 0.5 μm of ceramic film component with the flow velocity of 0.2m/s and carries out micro-filtration, under 0.16MPa pressure differentials, uses cut successively The membrane module of molecular weight 50000Da, 1O000Da are stayed, loop ultrafiltration processing is carried out with cross-current flow, obtaining molecular weight ranges is The Herb Gynostemmae Pentaphylli extract of 12000Da, 4.21mg/g containing saponin(e, flavonoids 2.48mg/g.
It further illustrates, the Cacumen Platycladi extract obtains addition 75% (v/v) ethyl alcohol after cacumen biotae is crushed molten Liquid carries out cycle extraction and obtains extracting solution, and it is 2.2 that extracting solution is added in salt acid for adjusting pH, then while stirring heating water bath until Occur filiform in extracting solution and start timing continuing stirring 8 minutes, filter to take filtrate in time, filter residue repeats said extracted step 2 It is secondary, Cacumen Platycladi extract is can obtain after the filtrate merging of 3 times is dried under reduced pressure, is crushed, contains flavones 1.35mg/g, terpene Class substance 1.08mg/g, tannin 11.1mg/g；
After Uricularia polytricha is dried the Uricularia polytricha extract, 75% (v/v) ethanol solution of 8 times of volumes is added to use rope When progress surname extraction 2.5 is small after family name's extractor, after 0.015% chitosan solution for filtering to take filtrate addition filtrate gross mass Stirring filters off filtrate and carries out heating concentration, Uricularia polytricha extract is freeze-dried to obtain, containing Uricularia polytricha until filtrate clarification Polysaccharide 147.25mg/g.
It further illustrates, the sweetener is xylitol or oligoisomaltose.
Layer of green-shell egg disease rate by above method raising is 2.2%.
Experiment one：
Zoopery：
1. experiment material and method
1.1 experimental subjects
1.1.1 sample treatment
Test sample will be that the plant extracts mixture of preparation of 1-3 is dissolved in distilled water, dilute various concentration, experiment When with physiological saline by sample preparation into each dosage.
1.1.2 experimental animal and detection environmental condition
Wistar rats are used in experiment, male, healthy cleaning grade, and weight 200-250g is totally 100, big by Guangxi medical courses in general Experimental Animal Center is learned to provide.Detection environmental condition, 20-25 DEG C of temperature range, RH range 50-70%, rat are being tried It is adapted to one week in animal house before testing.
1.2 instruments and reagent
Centrifuge
1.2.2 reagent
Cholesterol, cholate, serum total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL-C) measure reagent Box.Kit is purchased from Nanjing and builds up Bioengineering Research Institute.
1.3 high lipid food formulas
75% basal feed, 4% cholesterol, 10% yolk powder, 10% lard, 1% cholate.
1.4 dosage are grouped and given the test agent gives the time
Test three dosage groups for setting plant extracts mixture difference prepared by embodiment 1-3 and a control high in fat Group is respectively three dosage groups with the 5 of human body recommended amounts, 10,20 times.It is preventative to the given the test agent time and therapeutic to tested Sample time is 30d.
1.5 experimental procedure
Hyperlipoidemia method-preventative to given the test agent
Rat feeding basal feed observes 5-10d under experimental situation, and then afterbody takes blood, measures serum total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C) are horizontal.It is horizontal according to serum total cholesterol (TC), at random It is divided into 10 groups, every group 10, the given the test agent of various dose is given while high lipid food is given, wherein, control group high in fat (high lipid food+physiological saline), embodiment 1-3 are each 3 dosage of receptor, are low dose group (high lipid food+tested respectively Solution 1), middle dose group (high lipid food+test solution 2), high dose group (high lipid food+test solution 3), if basic, normal, high dose In amount group the concentration of given the test agent solution 1,2,3 be respectively 1.35,2.7,5.4g/kgbw (be respectively equivalent to human body recommended amounts 5,10,20 times), you can obtain A1 low dose groups (high lipid food+embodiment 1 prepare plant extracts mixture dissolved matter 1), A2 middle dose groups (plant extracts mixture dissolved matter 2 prepared by high lipid food+embodiment 1), A3 high dose groups are (high in fat Feed+embodiment 1 prepare plant extracts mixture dissolved matter 3), B1 low dose groups (high lipid food+embodiment 2 prepare Plant extracts mixture dissolved matter 1), B2 middle dose groups (high lipid food+embodiment 2 prepare plant extracts mixture it is molten Solve object 2), B3 high doses group (high lipid food+embodiment 2 prepare plant extracts mixture dissolved matter 3), C1 low dose groups (plant extracts mixture dissolved matter 1 prepared by high lipid food+embodiment 3), C2 middle dose groups (make by high lipid food+embodiment 3 Standby plant extracts mixture dissolved matter 2), C3 high doses group (high lipid food+embodiment 3 prepare plant extracts mixing Expect dissolved matter 3), gavage amount is 1.0mL/100gbw.Once a day, continuous 30 days, weigh once weekly, and adjustment fills according to this Stomach amount.Experiment measures serum TC, TG, HDL-C content, each group Rat Fast 16h before blood sampling to taking a blood sample respectively at the 28th day.Serum The measure of TC, TG and HDL-C content uses kit.
1.6 experimental datas count
Data summarization and analysis are carried out using spss19.0 and sigmaplot11.0 softwares.
2 experimental results
Influence of the 2.1 auxiliary reducing blood lipid plant extracts mixtures to rat body weight
Table 1 aids in influence of the reducing blood lipid plant extracts mixture to rat body weight
By table 1 as it can be seen that rat is to (0d) before tested material, each group weight is not significantly different.To the tested material regular period After (7d, 14d, 21d, 28d), compared with control group high in fat, remaining each group weight does not occur significant difference (p ＞ 0.05).Table The bright auxiliary reducing blood lipid plant extracts mixture does not have harmful effect to rat growthing development.
Influence of the 2.2 auxiliary reducing blood lipid plant extracts mixtures to rat blood serum T-CHOL (TC) content
Table 2 aids in influence of the reducing blood lipid plant extracts mixture to rat blood serum T-CHOL (TC) content
By table 2 as it can be seen that rat is given before tested material (0d), each experimental group serum total cholesterol (TC) content is compareed with high in fat Group compares that there was no significant difference (p ＞ 0.05).After the tested material regular period (7d, 14d, 21d, 28d), each period control high in fat For group compared with to before tested material, serum TC rise, difference has pole conspicuousness (p ＜ 0.01), shows high TC model foundations success. Compared with control group high in fat, remaining each dosage group significantly reduces (p ＜ 0.05).Show that the auxiliary reducing blood lipid plant extracts mixes Closing material plays an important role of to reduce serum total cholesterol (TC) content.
Influence of the 2.3 auxiliary reducing blood lipid plant extracts mixtures to rat blood serum triglycerides (TG) content
Table 3 aids in influence of the reducing blood lipid plant extracts mixture to rat blood serum triglycerides (TG) content
By table 3 as it can be seen that rat is given before tested material (0d), each experimental group serum levels of triglyceride (TG) content is compareed with high in fat Group compares that there was no significant difference (p ＞ 0.05).After the tested material regular period (7d, 14d, 21d, 28d), each period control high in fat For group compared with to before tested material, serum TG rise, difference has pole conspicuousness (p ＜ 0.01), shows high TG model foundations success. Compared with control group high in fat, remaining each dosage group significantly reduces (p ＜ 0.05).Show that the auxiliary reducing blood lipid plant extracts mixes Closing material plays an important role of to reduce serum levels of triglyceride (TG) content.
Influence of the 2.4 auxiliary reducing blood lipid plant extracts mixtures to rat high-density lipoprotein (HDL-C) content
Table 4 aids in influence of the reducing blood lipid plant extracts mixture to rat high-density lipoprotein (HDL-C) content
By table 4 as it can be seen that before giving tested material (0d), each experimental group high-density lipoprotein (HDL) content and control group high in fat Compare that there was no significant difference (p ＞ 0.05).After the tested material regular period (7d, 14d, 21d, 28d), each period control group high in fat Compared with to before tested material, high-density lipoprotein (HDL) content rise, difference has pole conspicuousness (p ＜ 0.01), shows height The success of HDL-C model foundations.Compared with control group high in fat, remaining each dosage group significantly reduces (p ＜ 0.05).Illustrate to show this Auxiliary reducing blood lipid plant extracts mixture plays an important role of to increase high-density lipoprotein (HDL) content.
According to《Function of health food assessment process and the method for inspection》Criterion to health-care food for assisting blood fat lowering (results of animal judgement) is to auxiliary lipid-lowering function result judgement：In serum total cholesterol, triglycerides, high density lipoprotein level Serum total cholesterol and triglycerides binomial index are positive in three Indexs measures of white cholesterol, can determine that the given the test agent aids in Hypolipemic function results of animal is positive.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair Bright patent claim, the equal variation or modification change completed under the technical spirit suggested by all present invention, should all belong to Cover the scope of the claims in the present invention.

Claims (10)

1. a kind of cultural method of layer of green-shell egg, which is characterized in that operate in accordance with the following steps：
For the layer of green-shell egg in 2-3 week old, the feed configured according to the raw material of following mass percentage is fed, 3 times/day, and 100-300g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Corn flour 45-55%, plant extracts mixture 5-10%, oyster shell whiting 8-15%, dregs of beans 10-15%, fermentation eggshell material 5-10%, fish meal 3-5%, salt 1-3%, calcium monohydrogen phosphate 2-4%；
The fermentation eggshell material is after discarded eggshell is crushed, and adds in mother-in-law U.S. degree as the 10-20 that ferments after the brown sugar water of 30-45 It；
The plant extracts mixture is made according to percent by weight of following raw material：Folum Ilicis extract 15-20%, purple Boisiana extract 8-15%, ophiopogon japonicus extract 10-18%, Stenoloma chusana extract 10-15%, sorosis extract 4-9%, chicory extract 5-13%, cranberry extract 7-12%, Herb Gynostemmae Pentaphylli extract 7-12%, Cacumen Platycladi extract 3-10%, Uricularia polytricha extract 3- 5%；
The preparation method of the plant extracts mixture is：It is added in after above-mentioned extract is mixed according to percent by weight After the 10-30% water of total weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added in：Hydroxymethyl cellulose： Dope is 0.15-0.23 according to ratio of weight and number:0.55-0.65:After 10-12 is stirred evenly, it is put into drying machine and is dried, The use of power is 50-80W while drying, frequency is that 30-50 hertz of ultrasonic waves are modified processing, you can obtain powdered plant Extract mixture.
2. the cultural method of layer of green-shell egg according to claim 1, which is characterized in that the Folum Ilicis extract will be fresh Ilex Latifolia Thunb carries out infusion 30-40 minutes after adding in the water of 5-10 times of volume, is filtered while hot, takes filtrate, by the progress of filtrate Heating stops heating after being concentrated into 1.02 times of original volume, obtains medicinal extract and is subsequently added into cellulase, 70-80% (v/v) ethyl alcohol Solution carry out surname extraction 1-2 it is small when, filtrate is dried under reduced pressure after filtering, crush after can obtain Folum Ilicis extract, For use；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 5-10:1-2:21-31.
3. the cultural method of layer of green-shell egg according to claim 1, which is characterized in that the Chinese wistaria extract is purple by 1kg After rattan seed crushes, supercritical CO is put into2In extraction equipment, when extraction 3-4 is small, extracting solution is collected, filter residue is added in and is filtered 70-80% (v/v) ethanol solution of 3-5 times of slag amount, carries out refluxing extraction 20-30 minutes, and the temperature of reflux is 65-85 DEG C Under, 0.3 μm of ceramic film component is then poured into the flow velocity of 0.1-0.2m/s and carries out micro-filtration, under 0.1-0.2MPa pressure differentials, according to The secondary membrane module with molecular cut off 10000Da, 5000Da carries out loop ultrafiltration processing with cross-current flow, obtains molecular weight model Enclose the Chinese wistaria extract for 5000Da-6000Da.
4. the cultural method of layer of green-shell egg according to claim 1, which is characterized in that the ophiopogon japonicus extract is by Radix Ophiopogonis 60-80% (v/v) ethanol solution that 3-5 times of powder weight is added in after pulverizing carries out refluxing extraction 3 times, every time 20 points Clock places water bath method in evaporating dish after filtering, be then dried under reduced pressure to 1.01 times of original volume, addition contains 1-2% After 70-80% (v/v) ethanol solution of acetic acid solution carries out constant volume, the speed using flow velocity as 0.1-0.2m/s carries out aperture and is 0.5-0.8 μm of miillpore filter is filtered, you can obtains ophiopogon japonicus extract.
5. the cultural method of layer of green-shell egg according to claim 1, which is characterized in that the Stenoloma chusana extract is by fresh crow The distilled water that 3-5 times of volume is added in after the removal of impurities of fragrant-flowered garlic herb carries out homogeneous mashing, be subsequently added into solvent extract 1-3 it is small when, connect The active material for carrying out that micro-filtration molecular cut off is 1000-2000Da, in rotating speed is 2000-3000r/min's by trapped fluid Separation 3-5 minutes is carried out in seperator, supernatant is taken to can obtain Stenoloma chusana extract after being spray-dried.
6. the cultural method of layer of green-shell egg according to claim 1, which is characterized in that the sorosis extract is complete by sorosis The water that grass adds in 2-3 times of volume is stirred into slurry in mixer, is subsequently added into progress water-bath after 3-5 times of water stirs evenly and carries It takes 30-60 minutes, thick liquid is obtained by filtration, thick liquid is poured into polyamide chromatography column with the flow velocity of 0.1-0.2m/s often chromatographs, and makes 3 elutions are carried out with 70-80% (v/v) ethanol solution, the pressure of elution is followed successively by -0.1MPa, 0.1MPa, 0.2MPa, elution Temperature for 60-70 DEG C, be then filtered, be concentrated under reduced pressure, dry, crush after obtain sorosis extract.
7. the cultural method of layer of green-shell egg according to claim 1, which is characterized in that the chicory extract is by witloof Flower adds in 5-10 times of volume water after smashing to pieces carries out water-bath extraction 20-30 minutes, and extraction process frequency of use is 200-300 hertz Microwave carry out assisted extraction, be concentrated under reduced pressure into 1.02 times of original volume after filtering, add in 20-30% (v/v) first Alcoholic solution carries out 3 elutions, and it is 60-70 DEG C that the pressure of elution, which is followed successively by -0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution, Then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain chicory extract.
8. the cultural method of layer of green-shell egg according to claim 1, which is characterized in that the cranberry extract puts blueberry Enter mixer and be stirred crushing, then carry out alcohol extracting at temperature is -5--3 DEG C using 65-75% (v/v) ethanol solutions Night uses rotating speed to be centrifuged for the centrifuge of 300-400r/min and thick liquid is obtained by filtration, by thick liquid and albumen after overnight When room temperature extraction 1-3 is small after enzyme mixing, cranberry extract is obtained by filtration；
The water that gynostemma pentaphylla is added in 2-5 times of volume by the Herb Gynostemmae Pentaphylli extract is smashed to pieces, is smashed to pieces into after slurry and is using electric field strength The high-pressure pulse electric processing of 25KV/cm, the time of pulse is 5 × 10-6-10×10-6S, the frequency of pulse is 30-50 hertz, 5-10 times 70-80% (v/v) ethanol solution is added in after being handled and carries out ultrasonic extraction 10-20 minutes using Ultrasound Instrument, is surpassed The current strength of sound instrument is 5A/25W-10A/25W, and then pouring into 0.5 μm of ceramic film component with the flow velocity of 0.1-0.25m/s carries out Micro-filtration, under 0.1-0.2MPa pressure differentials, successively with the membrane module of molecular cut off 50000Da, 1O000Da, with cross-current flow Loop ultrafiltration processing is carried out, obtains the Herb Gynostemmae Pentaphylli extract that molecular weight ranges are 10000Da-15000Da.
9. the cultural method of layer of green-shell egg according to claim 1, which is characterized in that the Cacumen Platycladi extract is by arbor-vitae Leaf obtained after crushing add in 70-80% (v/v) ethanol solution carry out cycle extraction obtain extracting solution, extracting solution is added in into salt Acid for adjusting pH is 2.0-3.0, and then heating water bath continues to stir until filiform occur in extracting solution and starting timing while stirring 5-10 minutes, filtrate was filtered to take in time, filter residue repeats said extracted step 2 time, the filtrate merging of 3 times is dried under reduced pressure, Cacumen Platycladi extract is can obtain after crushing；
After Uricularia polytricha is dried the Uricularia polytricha extract, adding in 70-80% (v/v) ethanol solution of 5-10 times of volume makes With carried out after apparatus,Soxhlet's surname extraction 2-3 it is small when, filter to take filtrate and add in the shell of 0.01-0.02% of filtrate gross mass and gather Stirring after sugar juice filters off filtrate and carries out heating concentration, be freeze-dried to obtain Uricularia polytricha extract until filtrate clarification.
10. the cultural method of layer of green-shell egg according to claim 1, which is characterized in that the sweetener for xylitol or Oligoisomaltose.
CN201711344038.0A 2017-12-15 2017-12-15 A kind of cultural method of layer of green-shell egg Pending CN108056063A (en)

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CN104082540A (en) * 2014-05-28 2014-10-08 青岛华仁技术孵化器有限公司 Feed for health-care egg
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CN104082540A (en) * 2014-05-28 2014-10-08 青岛华仁技术孵化器有限公司 Feed for health-care egg
CN105230989A (en) * 2015-11-11 2016-01-13 覃政强 Chicken feed and application thereof
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