CN107821332A - A kind of cultural method of native duck - Google Patents
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- CN107821332A CN107821332A CN201711344051.6A CN201711344051A CN107821332A CN 107821332 A CN107821332 A CN 107821332A CN 201711344051 A CN201711344051 A CN 201711344051A CN 107821332 A CN107821332 A CN 107821332A
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Abstract
The present invention relates to cultural technique field, more particularly to a kind of cultural method of native duck, operate in accordance with the following steps：For the native duck in 23 week old, the feed obtained using configuration is fed, 3 times/day, and 100 300g/ every time, and by its free choice feeding to all you can eat；The composition of the feed is as follows：Oyster shell whiting 50 60%, shrimp powder 10 15%, soybean residue 8 15%, fermentation purple napier grass 5 8%, fish meal 2 5%, salt 1 3%, calcium monohydrogen phosphate 3 5%, reducing blood lipid compound 2 5%.Advantage of the present invention：The feed for finding to feed the application in periodic time by substantial amounts of research can not only improve growth rate, a series of intercurrent disease caused by blood fat rise is also controlled well, and feed has preferable insecticidal action, ensure that native duck free choice feeding eats harmful substance intoxicating phenomenon by mistake, so as to improve the survival rate of native duck, and then increase the purpose of income.
Description
Technical field
The present invention relates to cultural technique field, more particularly to a kind of cultural method of native duck.
Background technology
Aliphatic acid fusing point in duck is low, is easy to digest.Contained B family vitamin and vitamin E are more compared with other meats, can have
Effect resistance athlete's foot, neuritis and inflammation, moreover it is possible to anti-aging.Contain the nicotinic acid compared with horn of plenty in duck, it is to form people
The heart disease patients such as myocardial infarction are had protective effect by one of composition of internal two kinds of important coenzyme.Population is edible
With suitable for thering is heat, the people to get angry to eat in vivo；Hair low-heat, the people have a delicate constitution, lose the appetite, being hard and dry with oedema, food
More preferably.Adequate nutritional is bad simultaneously, and physically weak after postpartum disease, night sweat, seminal emission, woman in menstrual period are few, dry throat and mouth person eats；It is also suitable
After suitable cancer patient and radiotherapy chemotherapy, diabetes, cirrhotic ascites, pulmonary tuberculosis, chronic nephritis edema person eats.It is empty for ferritic
Cold, caused do not feel like eating of suffering from cold, stomach crymodynia, diarrhoea is thin, pain in the back and cold dysmenorrhoea and obesity, artery sclerosis, chronic
Enteritis should lack food；Cold patients' inedibility.So duck can not have high fat of blood, for this, urgent need finds a kind of both safety, and can is prevented
Only duck suffers from the sick extremely urgent with complication of high fat of blood.
The content of the invention
The goal of the invention of the present invention is：A kind of cultural method of native duck is provided, found by substantial amounts of research regular
The feed that time feeds the application can not only improve growth rate, also have a series of caused by control blood fat rise well
Intercurrent disease, and feed has preferable insecticidal action, ensures that native duck free choice feeding eats harmful substance intoxicating phenomenon by mistake, so as to improve
The survival rate of native duck, and then increase the purpose of income.
In order to realize foregoing invention purpose, the technical solution adopted by the present invention is as follows：
A kind of cultural method of native duck, is operated in accordance with the following steps：
For the native duck in 2-3 week old, the feed for configuring to obtain according to the raw material of following mass percentage is fed,
3 times/day, and 100-300g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Oyster shell whiting 45-55%, reducing blood lipid compound 5-10%, shrimp powder 8-15%, soya bean
Slag 10-15%, fermentation purple napier grass 5-10%, fish meal 3-5%, salt 1-3%, calcium monohydrogen phosphate 2-4%；
The reducing blood lipid compound is made up according to percent by weight of following raw material：Folum Ilicis extract 10-18%, pool
Extract 10-20%, Herba Patriniae extract 8-15%, strong wind seed extract 8-15%, sorosis extract 5-10%, garlic is rushed down to carry
Take thing 5-10%, Radix Gentianae extract 5-10%, Herb Gynostemmae Pentaphylli extract 3-10%, Radix Sangusorbae extract 5-12%, Uricularia polytricha extract
5-10%；
The preparation method of the reducing blood lipid compound is：Added after above-mentioned extract is mixed according to percent by weight
After the 10-30% water of gross weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added：Hydroxymethyl cellulose：
Dope is 0.15-0.23 according to ratio of weight and number:0.55-0.65:After 10-12 stirs, it is put into drying machine and is dried,
The use of power is 50-80W while drying, frequency be that 30-50 hertz ultrasonic waves are modified processing, you can obtain powdery drop blood
Fat compound.
Further illustrate, the water that the Ilex Latifolia Thunb activity extract adds fresh Ilex Latifolia Thunb herb 5-10 times of volume is laggard
Row infusion 30-40 minutes, filtered while hot, take filtrate, filtrate stop after heating is concentrated into 1.02 times of original volume
Only heat, obtain medicinal extract and be subsequently added into cellulase, 70-80% (v/v) ethanol solution progress surname extraction 1-2 hours, filtering
Filtrate is dried under reduced pressure afterwards, crush after can obtain Ilex Latifolia Thunb activity extract, containing amyrin 2.36-4.05mg/g,
Lupeol 2.36-4.67mg/g, cupreol 3.10-3.45mg/g, amaroid saponin(e 2.01-3.23mg/g；The medicinal extract：
Cellulase：The weight ratio of ethanol solution is 3-4:1-2:18-22.
Further illustrate, after the rhizoma alismatis activity extract is crushed 1kg rhizoma alismatis, put into supercritical CO2Extraction dress
In putting, 3-4 hours are extracted, collect extract solution, filter residue is added to 70-80% (v/v) ethanol solution of 3-5 times of filter residue quality, entered
Row refluxing extraction 20-30 minutes, the temperature of backflow is at 65-85 DEG C, then pours into 0.3 μm of ceramics with 0.1-0.2m/s flow velocity
Membrane module carries out micro-filtration, under 0.1-0.2MPa pressure differentials, successively with molecular cut off 10000Da, 5000Da membrane module, with
Cross-current flow carries out loop ultrafiltration processing, the rhizoma alismatis activity extract that molecular weight ranges are 5000Da-6000Da is obtained, containing choline
1.31-2.42mg/g, lecithin 0.56-0.78mg/g, triterpene keto-alcohol 0.74-1.08mg/g.
Further illustrate, the field pennycress activity extract is that field pennycress is added into powder weight 3- after pulverizing
5 times 60-80% (v/v) ethanol solution carries out refluxing extraction 3 times, 20 minutes every time, and water-bath in evaporating dish is placed after filtering and is steamed
It is dry, then it is dried under reduced pressure that to 1.01 times of original volume, to add 70-80% (v/v) ethanol containing 1-2% acetic acid solutions molten
After liquid carries out constant volume, the speed using flow velocity as 0.1-0.2m/s carries out the miillpore filter that aperture is 0.5-0.8 μm and filtered, i.e.,
It can obtain field pennycress activity extract, 2.21-3.65mg/g containing volatile oil, scabioside 0.56-1.12mg/g.
Further illustrate, 3-5 times of volume of addition after the gynostemma pentaphylla activity extract cleans fresh gynostemma pentaphyllum herb
Distilled water carries out homogeneous mashing, is subsequently added into solvent and carries out extracting 1-3 hours, it is 1000- then to carry out micro-filtration molecular cut off
2000Da active material, trapped fluid is subjected to separation 3-5 minutes in the seperator that rotating speed is 2000-3000r/min, taken
Gynostemma pentaphylla activity extract is can obtain after clear liquid spray drying, 3.15-6.47mg/g containing saponin(e, flavonoids in obtained material
3.15-4.07mg/g。
Further illustrate, chaulmoogra herb is added the water of 2-3 times of volume in mixer by the chaulmoogra activity extract
In be stirred into slurry, be subsequently added into 3-5 times of water and stir laggard water-filling bath extraction 30-60 minutes, be filtrated to get thick liquid, will
Thick liquid pours into polyamide chromatography post with 0.1-0.2m/s flow velocity and often chromatographed, and 3 are carried out using 70-80% (v/v) ethanol solution
Secondary elution, it is 60-70 DEG C that the pressure of elution, which is followed successively by -0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution, is then carried out
Filter, be concentrated under reduced pressure, dry, crush after obtain chaulmoogra activity extract, 2.56-5.34mg/g containing alkaloid, crude fibre
10.26-25.36%.
Further illustrate, the sorosis activity extract adds 5-10 times of volume water after the flower of sorosis is smashed to pieces enters water-filling
Bath extraction 20-30 minutes, extraction process frequency of use carry out assisted extraction for the microwave of 200-300 hertz, subtracted after filtering
Pressure is concentrated into 1.02 times of original volume, adds 20-30% (v/v) methanol solution and carries out 3 elutions, the pressure of elution is successively
For -0.1MPa, 0.1MPa, 0.2MPa, elution temperature be 60-70 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after
To sorosis activity extract, 2.01-3.24mg/g containing tannic acid, anthocyanidin 2.54-4.27mg/g.
Further illustrate, garlic is put into mixer and is stirred crushing by the garlic activity extract, then using 65-
75% (v/v) ethanol solution is to carry out alcohol extracting at -5--3 DEG C to stay overnight in temperature, and rotating speed is used as 300-400r/min's after overnight
Centrifuge, which is centrifuged, is filtrated to get thick liquid, and normal temperature extracts 1-3 hours after thick liquid is mixed with protease, is filtrated to get big
Garlic activity extract, 12.69-31.24mg/g containing allicin；
The water that the rough gentian activity extract adds rough gentian 2-5 times of volume is smashed to pieces, and electric-field intensity is used after smashing into slurry to pieces
For
25KV/cm high-pressure pulse electric processing, the time of pulse is 5 × 10-6-10×10-6S, the frequency of pulse are
30-50 hertz, 5-10 times 70-80% (v/v) ethanol solution is added after being handled and carries out ultrasonic extraction 10- using Ultrasound Instrument
20 minutes, the current strength of Ultrasound Instrument was 5A/25W-10A/25W, then pours into 0.5 μm of ceramics with 0.1-0.25m/s flow velocity
Membrane module carries out micro-filtration, under 0.1-0.2MPa pressure differentials, successively with molecular cut off 50000Da, 1O000Da membrane module,
Loop ultrafiltration processing is carried out with cross-current flow, the rough gentian activity extract that molecular weight ranges are 10000Da-15000Da is obtained, contains
Courage hardship glycosides 4.26-5.32mg/g, chiratin 4.21-5.86mg/g.
Further illustrate, the garden burnet activity extract obtains adding 70-80% (v/v) after garden burnet stem tuber is crushed
Ethanol solution, which carries out circulating extraction, obtains extract solution, is 2.0-3.0 by extract solution addition salt acid for adjusting pH, then water while stirring
Bath heating continues to stir 5-10 minutes until occurring filiform in extract solution and starting timing, filters to take filtrate in time, filter residue repeats
Said extracted step 2 time, garden burnet activity extract is can obtain after the filtrate merging of 3 times is dried under reduced pressure, crushed, containing three
Terpene 1.25-2.32mg/g, saponins material 1.56-1.94mg/g, tannin class material 1.56-2.17mg/g；
After Uricularia polytricha is dried the Uricularia polytricha activity extract, 70-80% (v/v) second of 5-10 times of volume is added
Alcoholic solution carries out surname extraction 2-3 hours after using apparatus,Soxhlet's, filters to take the 0.01- that filtrate adds filtrate gross mass
Stirring after 0.02% chitosan solution filters off filtrate and carries out heating concentration, be freeze-dried to obtain until filtrate clarification
Uricularia polytricha activity extract, 115.26-157.36mg/g containing Polysaccharide from Auricularia Polytricha Sacc.
Further illustrate, the sweetener is xylitol or oligoisomaltose.
In summary, by adopting the above-described technical solution, the beneficial effects of the invention are as follows：
Oyster shell whiting of the present invention, shrimp powder, soybean residue, fermentation purple napier grass, fish meal, salt, calcium monohydrogen phosphate, reducing blood lipid mixing
Material has reached after proportioning contains organic matter 51.26-59.34%, micro- 8.11-9.37%, effective carbon 21.36-
34.26%th, effective calcium 17.26-18.21% can meet the nutrient needed for native duck growth, promote growth, and with the addition of
It is common to make rich in substantial amounts of Flavonoid substances, alkaloid, benzine soap glycoside material, tannin class material in reducing blood lipid compound
Cause native duck that there is preferably resistance energy after free choice feeding other plant with the phenomenon for playing desinsection, antimicrobial flora grows
Power, and increase by free choice feeding the amount of exercise of native duck, building up health reduces sick rate, and moderate exercise auxiliary reduces grease accumulation
So that delicious meat and non-greasy, and the reducing blood lipid compound added has the function that good effect for reducing blood fat, improves soil
The survival rate of duck.
The effect of each reducing blood lipid compound：Ilex Latifolia Thunb wind and heat dispersing, relieving restlessness cough-relieving, eliminate indigestion and phlegm；It is rhizoma alismatis diuresis, clear
It is damp and hot；Field pennycress is clearing heat and detoxicating, removing blood stasis and expelling pus；Chaulmoogra dispels pathogenic wind and remove dampness, detoxicating and destroying parasites；Sorosis is enriched blood enriching yin, fluid dryness；Greatly
Garlic middle benefit gas stomach invigorating, help digestion qi-regulating；Rough gentian clearing away the liver-fire, except damp and hot, stomach invigorating；Garden burnet is clearing heat and detoxicating, cooling blood and hemostasis, healing up sore and subduing swelling；Twist
The blue clearing heat and detoxicating, cough-relieving apophlegmatic of stock, strong benefiting action.10 taste medicine collective effects, complement each other, containing substantial amounts of in 10 taste medicines
Active component, the technological means by the application carry out the position active material in extraction Chinese medicine, are rich in active material a large amount of
The effective active composition and trace element that can play a part of reduction to blood fat, trace element the function of human body can be played
The effect of nutrient environment in balanced body, it can preferably carry out reducing the treatment of blood fat；So as to prevent a variety of to be caused by cholesterol
Syndrome, play the effect for reducing native duck sick rate well.
Embodiment
All features disclosed in this specification, or disclosed all methods or during the step of, except mutually exclusive
Feature and/or step beyond, can combine in any way.This specification (including any accessory claim, summary)
Disclosed in any feature, unless specifically stated otherwise, can alternative features equivalent by other or with similar purpose replaced.
I.e., unless specifically stated otherwise, each feature is an example in a series of equivalent or similar characteristics.
Embodiment 1
A kind of cultural method of native duck, is operated in accordance with the following steps：
For the native duck in 2 week old, the feed for configuring to obtain according to the raw material of following mass percentage is fed, and 3
Times/day, and 100g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：The composition of the feed is as follows：Oyster shell whiting 45%, reducing blood lipid compound 10%, shrimp
Skin powder 8%, soybean residue 15%, fermentation purple napier grass 10%, fish meal 5%, salt 3%, calcium monohydrogen phosphate 4%；
The reducing blood lipid compound is made up according to percent by weight of following raw material：Folum Ilicis extract 14%, rhizoma alismatis
Extract 15%, Herba Patriniae extract 11%, strong wind seed extract 12%, sorosis extract 7%, garlic P.E 6%, rough gentian
Extract 8%, Herb Gynostemmae Pentaphylli extract 8%, Radix Sangusorbae extract 10%, Uricularia polytricha extract 9%；
The preparation method of the reducing blood lipid compound is：Added after above-mentioned extract is mixed according to percent by weight
After 10% water of gross weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added：Hydroxymethyl cellulose：It is sticky
Thing is 0.15 according to ratio of weight and number:0.55:After 10 stir, it is put into drying machine and is dried, uses work(while drying
Rate is 50W, frequency is that 30 hertz of ultrasonic waves are modified processing, you can obtains powdery reducing blood lipid compound.
Further illustrate, the Ilex Latifolia Thunb activity extract is carried out after fresh Ilex Latifolia Thunb herb to be added to the water of 5 times of volumes
Infusion 30 minutes, is filtered, takes filtrate while hot, by filtrate stop after heating is concentrated into 1.02 times of original volume plus
Heat, obtain medicinal extract and be subsequently added into cellulase, 70% (v/v) ethanol solution progress surname extraction 1 hour, enter filtrate after filtering
Row be dried under reduced pressure, crush after can obtain Ilex Latifolia Thunb activity extract, contain amyrin 2.36mg/g, lupeol 2.36mg/
G, cupreol 3.10mg/g, amaroid saponin(e 2.01mg/g；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 3:
1：18.
Further illustrate, after the rhizoma alismatis activity extract is crushed 1kg rhizoma alismatis, put into supercritical CO2Extraction dress
In putting, extract 3 hours, collect extract solution, filter residue is added to 70% (v/v) ethanol solution of 3 times of filter residue quality, backflow is carried out and carries
Taking 20 minutes, the temperature of backflow is at 65 DEG C, then pours into 0.3 μm of ceramic film component with 0.1m/s flow velocity and carries out micro-filtration,
Under 0.1MPa pressure differentials, successively with molecular cut off 10000Da, 5000Da membrane module, loop ultrafiltration is carried out with cross-current flow
Processing, obtain the rhizoma alismatis activity extract that molecular weight ranges are 5000Da, 1.31mg/g containing choline, lecithin 0.56mg/g, three
Terpene keto-alcohol 0.74mg/g.
Further illustrate, the field pennycress activity extract is that field pennycress is added into powder weight 3 after pulverizing
60% (v/v) ethanol solution again carries out refluxing extraction 3 times, 20 minutes every time, and water bath method in evaporating dish is placed after filtering, is connect
And be dried under reduced pressure to 1.01 times of original volume, add 70% (v/v) ethanol solution containing 1% acetic acid solution and carry out constant volume
Afterwards, the miillpore filter that the speed progress aperture using flow velocity as 0.1m/s is 0.5 μm is filtered, you can is obtained field pennycress activity and is carried
Thing is taken, 2.21mg/g containing volatile oil, scabioside 0.56mg/g.
Further illustrate, the gynostemma pentaphylla activity extract adds the steaming of 3 times of volumes after fresh gynostemma pentaphyllum herb is cleaned
Distilled water carries out homogeneous mashing, is subsequently added into solvent and carries out extraction 1 hour, then carries out the work that micro-filtration molecular cut off is 1000Da
Property material, by trapped fluid rotating speed be 2000r/min seperator in carry out separation 3 minutes, take supernatant be spray-dried after i.e.
Gynostemma pentaphylla activity extract is can obtain, 3.15mg/g containing saponin(e, flavonoids 3.15mg/g in obtained material.
Further illustrate, chaulmoogra herb is added the water of 2 times of volumes in mixer by the chaulmoogra activity extract
Be stirred into slurry, be subsequently added into 3 times of water and stir laggard water-filling bath extraction 30 minutes, be filtrated to get thick liquid, by thick liquid with
0.1m/s flow velocity pours into polyamide chromatography post and often chromatographed, and 3 elutions are carried out using 70% (v/v) ethanol solution, elution
It is 60 DEG C that pressure, which is followed successively by -0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution, is then filtered, be concentrated under reduced pressure, dry,
Chaulmoogra activity extract, 2.56mg/g containing alkaloid, crude fibre 10.26% are obtained after crushing.
Further illustrate, the sorosis activity extract adds 5 times of volumes water after the flower of sorosis is smashed to pieces carries out water-bath
Extraction 20 minutes, the microwave that extraction process frequency of use is 200 hertz carry out assisted extraction, carry out being concentrated under reduced pressure into original after filtering
1.02 times of volume, add 20% (v/v) methanol solution and carry out 3 elutions, the pressure of elution be followed successively by -0.1MPa,
0.1MPa, 0.2MPa, the temperature of elution are 60 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain sorosis activity and carry
Thing is taken, 2.01mg/g containing tannic acid, anthocyanidin 2.54mg/g.
Further illustrate, garlic is put into mixer and is stirred crushing, then used by the garlic activity extract
65% (v/v) ethanol solution is to carry out alcohol extracting at -5 DEG C to stay overnight in temperature, and centrifuge of the rotating speed for 300r/min is used after overnight
It is centrifuged and is filtrated to get thick liquid, normal temperature extracts 1 hour after thick liquid is mixed with protease, is filtrated to get garlic activity and carries
Thing is taken, 12.69mg/g containing allicin；
The water that the rough gentian activity extract adds rough gentian 2 times of volumes is smashed to pieces, is smashed to pieces into after slurry and is using electric-field intensity
25KV/cm high-pressure pulse electric processing, the time of pulse is 5 × 10-6S, the frequency of pulse is 30 hertz, is added after being handled
Enter 5 times 70% (v/v) ethanol solution and carry out ultrasonic extraction 10 minutes using Ultrasound Instrument, the current strength of Ultrasound Instrument is 5A/
25W, 0.5 μm of ceramic film component is then poured into 0.1m/s flow velocity and carries out micro-filtration, under 0.1MPa pressure differentials, successively with retention
Molecular weight 50000Da, 1O000Da membrane module, loop ultrafiltration processing is carried out with cross-current flow, obtaining molecular weight ranges is
10000Da rough gentian activity extract, the glycosides of hardship containing courage 4.26mg/g, chiratin 4.21mg/g.
Further illustrate, the garden burnet activity extract obtains addition 70% (v/v) second after garden burnet stem tuber is crushed
Alcoholic solution, which carries out circulating extraction, obtains extract solution, and it is 2.0 that extract solution is added into salt acid for adjusting pH, then heating water bath while stirring
Continue stirring 5 minutes until occurring filiform in extract solution and starting timing, filter to take filtrate in time, filter residue repeats said extracted step
Rapid 2 times, garden burnet activity extract, 1.25mg/ containing triterpene are can obtain after the filtrate merging of 3 times is dried under reduced pressure, crushed
G, saponins material 1.56mg/g, tannin class material 1.56mg/g；
After Uricularia polytricha is dried the Uricularia polytricha activity extract, 70% (v/v) ethanol solution of 5 times of volumes is added
Using surname extraction is carried out after apparatus,Soxhlet's 2 hours, 0.01% chitosan for filtering to take filtrate addition filtrate gross mass is molten
Stirring after liquid filters off filtrate and carries out heating concentration, be freeze-dried to obtain Uricularia polytricha activity extract until filtrate clarification
115.26mg/g containing Polysaccharide from Auricularia Polytricha Sacc.
Further illustrate, the sweetener is xylitol.
The disease rate of native duck by above method raising is 0.8%.
Embodiment 2
A kind of cultural method of native duck, is operated in accordance with the following steps：
For the native duck in 3 week old, the feed for configuring to obtain according to the raw material of following mass percentage is fed, and 3
Times/day, and 300g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Oyster shell whiting 55%, reducing blood lipid compound 5%, shrimp powder 15%, soybean residue 10%,
Ferment purple napier grass 9%, fish meal 3%, salt 1%, calcium monohydrogen phosphate 2%；
The reducing blood lipid compound is made up according to percent by weight of following raw material：Folum Ilicis extract 10%, rhizoma alismatis
Extract 10%, Herba Patriniae extract 8%, strong wind seed extract 15%, sorosis extract 5%, garlic P.E 10%, rough gentian
Extract 10%, Herb Gynostemmae Pentaphylli extract 10%, Radix Sangusorbae extract 12%, Uricularia polytricha extract 10%；
The preparation method of the reducing blood lipid compound is：Added after above-mentioned extract is mixed according to percent by weight
After 30% water of gross weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added：Hydroxymethyl cellulose：It is sticky
Thing is 0.23 according to ratio of weight and number:0.65:After 12 stir, it is put into drying machine and is dried, uses work(while drying
Rate is 80W, frequency is that 50 hertz of ultrasonic waves are modified processing, you can obtains powdery reducing blood lipid compound.
Further illustrate, the Ilex Latifolia Thunb activity extract is carried out after fresh Ilex Latifolia Thunb herb to be added to the water of 8 times of volumes
Infusion 37 minutes, is filtered, takes filtrate while hot, by filtrate stop after heating is concentrated into 1.02 times of original volume plus
Heat, obtain medicinal extract and be subsequently added into cellulase, 78% (v/v) ethanol solution progress surname extraction 1.4 hours, by filtrate after filtering
Be dried under reduced pressure, crush after can obtain Ilex Latifolia Thunb activity extract, contain amyrin 3.16mg/g, lupeol
3.17mg/g, cupreol 3.27mg/g, amaroid saponin(e 2.75mg/g；The medicinal extract：Cellulase：The weight of ethanol solution
Than for 3:2:19.
Further illustrate, after the rhizoma alismatis activity extract is crushed 1kg rhizoma alismatis, put into supercritical CO2Extraction dress
In putting, extract 3.4 hours, collect extract solution, filter residue is added to 74% (v/v) ethanol solution of 4 times of filter residue quality, flowed back
Extraction 25 minutes, the temperature of backflow is at 71 DEG C, then pours into 0.3 μm of ceramic film component with 0.13m/s flow velocity and carries out micro-filtration,
Under 0.16MPa pressure differentials, successively with molecular cut off 10000Da, 5000Da membrane module, it is super that circulation is carried out with cross-current flow
Filter handle, obtain molecular weight ranges be 5500Da rhizoma alismatis activity extract, 1.87mg/g containing choline, lecithin 0.67mg/g,
Triterpene keto-alcohol 0.86mg/g.
Further illustrate, the field pennycress activity extract is that field pennycress is added into powder weight 4 after pulverizing
70% (v/v) ethanol solution again carries out refluxing extraction 3 times, 20 minutes every time, and water bath method in evaporating dish is placed after filtering, is connect
And be dried under reduced pressure to 1.01 times of original volume, add 74% (v/v) ethanol solution containing 1.5% acetic acid solution and determined
Rong Hou, the speed using flow velocity as 0.13m/s carry out the miillpore filter that aperture is 0.6 μm and filtered, you can obtain field pennycress work
Property extract, 3.04mg/g containing volatile oil, scabioside 0.92mg/g.
Further illustrate, the gynostemma pentaphylla activity extract adds the steaming of 4 times of volumes after fresh gynostemma pentaphyllum herb is cleaned
Distilled water carries out homogeneous mashing, is subsequently added into solvent and carries out extraction 2 hours, then carries out the work that micro-filtration molecular cut off is 1500Da
Property material, by trapped fluid rotating speed be 2400r/min seperator in carry out separation 4 minutes, take supernatant be spray-dried after i.e.
Gynostemma pentaphylla activity extract is can obtain, 5.21mg/g containing saponin(e, flavonoids 3.84mg/g in obtained material.
Further illustrate, chaulmoogra herb is added the water of 3 times of volumes in mixer by the chaulmoogra activity extract
Be stirred into slurry, be subsequently added into 4 times of water and stir laggard water-filling bath extraction 50 minutes, be filtrated to get thick liquid, by thick liquid with
0.15m/s flow velocity pours into polyamide chromatography post and often chromatographed, and 3 elutions are carried out using 75% (v/v) ethanol solution, elution
It is 65 DEG C that pressure, which is followed successively by -0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution, is then filtered, be concentrated under reduced pressure, dry,
Chaulmoogra activity extract, 5.36mg/g containing alkaloid, crude fibre 14.67% are obtained after crushing.
Further illustrate, the sorosis activity extract adds 6 times of volumes water after the flower of sorosis is smashed to pieces carries out water-bath
Extraction 25 minutes, the microwave that extraction process frequency of use is 250 hertz carry out assisted extraction, carry out being concentrated under reduced pressure into original after filtering
1.02 times of volume, add 25% (v/v) methanol solution and carry out 3 elutions, the pressure of elution be followed successively by -0.1MPa,
0.1MPa, 0.2MPa, the temperature of elution are 65 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain sorosis activity and carry
Thing is taken, 2.75mg/g containing tannic acid, anthocyanidin 3.64mg/g.
Further illustrate, garlic is put into mixer and is stirred crushing, then used by the garlic activity extract
70% (v/v) ethanol solution is to carry out alcohol extracting at -4 DEG C to stay overnight in temperature, and centrifuge of the rotating speed for 340r/min is used after overnight
It is centrifuged and is filtrated to get thick liquid, normal temperature extracts 3 hours after thick liquid is mixed with protease, is filtrated to get garlic activity and carries
Thing is taken, 21.36mg/g containing allicin；
The water that the rough gentian activity extract adds rough gentian 4 times of volumes is smashed to pieces, is smashed to pieces into after slurry and is using electric-field intensity
25KV/cm high-pressure pulse electric processing, the time of pulse is 7 × 10-6S, the frequency of pulse is 40 hertz, is added after being handled
Enter 7 times 76% (v/v) ethanol solution and carry out ultrasonic extraction 15 minutes using Ultrasound Instrument, the current strength of Ultrasound Instrument is 8A/
25W, 0.5 μm of ceramic film component is then poured into 0.16m/s flow velocity and carries out micro-filtration, under 0.13MPa pressure differentials, used cut successively
Molecular weight 50000Da, 1O000Da membrane module are stayed, loop ultrafiltration processing is carried out with cross-current flow, obtaining molecular weight ranges is
12000Da rough gentian activity extract, the glycosides of hardship containing courage 4.78mg/g, chiratin 5.02mg/g..
Further illustrate, the garden burnet activity extract obtains addition 80% (v/v) second after garden burnet stem tuber is crushed
Alcoholic solution, which carries out circulating extraction, obtains extract solution, and it is 3.0 that extract solution is added into salt acid for adjusting pH, then heating water bath while stirring
Continue stirring 10 minutes until occurring filiform in extract solution and starting timing, filter to take filtrate in time, filter residue repeats said extracted
Step 2 time, garden burnet activity extract is can obtain after the filtrate merging of 3 times is dried under reduced pressure, crushed, containing triterpene
2.32mg/g, saponins material 1.94mg/g, tannin class material 2.17mg/g；
After Uricularia polytricha is dried the Uricularia polytricha activity extract, 80% (v/v) ethanol for adding 10 times of volumes is molten
Liquid carries out surname extraction 3 hours after using apparatus,Soxhlet's, filters to take 0.02% chitosan that filtrate adds filtrate gross mass
Stirring after solution filters off filtrate and carries out heating concentration until filtrate clarification, is freeze-dried to obtain Uricularia polytricha activity extraction
Thing, 157.36mg/g containing Polysaccharide from Auricularia Polytricha Sacc.
Further illustrate, the sweetener is oligoisomaltose.
The disease rate of native duck by above method raising is 1.8%.
Embodiment 3
A kind of cultural method of native duck, is operated in accordance with the following steps：
For the native duck in 3 week old, the feed for configuring to obtain according to the raw material of following mass percentage is fed, and 3
Times/day, and 200g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Oyster shell whiting 52%, reducing blood lipid compound 9%, shrimp powder 12%, soybean residue 13%,
Ferment purple napier grass 5%, fish meal 4%, salt 2%, calcium monohydrogen phosphate 3%；
The reducing blood lipid compound is made up according to percent by weight of following raw material：Folum Ilicis extract 18%, rhizoma alismatis
Extract 20%, Herba Patriniae extract 15%, strong wind seed extract 8%, sorosis extract 10%, garlic P.E 5%, rough gentian
Extract 5%, Herb Gynostemmae Pentaphylli extract 4%, Radix Sangusorbae extract 5%, Uricularia polytricha extract 10%；
The preparation method of the reducing blood lipid compound is：Added after above-mentioned extract is mixed according to percent by weight
After 20% water of gross weight is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added：Hydroxymethyl cellulose：It is sticky
Thing is 0.2 according to ratio of weight and number:0.6:After 11 stir, it is put into drying machine and is dried, uses power while drying
It is that 40 hertz of ultrasonic waves are modified processing for 70W, frequency, you can obtain powdery reducing blood lipid compound.
Further illustrate, the Ilex Latifolia Thunb activity extract is carried out after fresh Ilex Latifolia Thunb herb to be added to the water of 10 times of volumes
Infusion 40 minutes, is filtered, takes filtrate while hot, by filtrate stop after heating is concentrated into 1.02 times of original volume plus
Heat, obtain medicinal extract and be subsequently added into cellulase, 80% (v/v) ethanol solution progress surname extraction 2 hours, enter filtrate after filtering
Row be dried under reduced pressure, crush after can obtain Ilex Latifolia Thunb activity extract, contain amyrin 4.05mg/g, lupeol 4.67mg/
G, cupreol 3.45mg/g, amaroid saponin(e 3.23mg/g；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 4:
2:22。
Further illustrate, after the rhizoma alismatis activity extract is crushed 1kg rhizoma alismatis, put into supercritical CO2Extraction dress
In putting, extract 4 hours, collect extract solution, filter residue is added to 80% (v/v) ethanol solution of 5 times of filter residue quality, backflow is carried out and carries
Taking 30 minutes, the temperature of backflow is at 85 DEG C, then pours into 0.3 μm of ceramic film component with 0.2m/s flow velocity and carries out micro-filtration,
Under 0.2MPa pressure differentials, successively with molecular cut off 10000Da, 5000Da membrane module, loop ultrafiltration is carried out with cross-current flow
Processing, obtain the rhizoma alismatis activity extract that molecular weight ranges are 6000Da, 2.42mg/g containing choline, lecithin 0.78mg/g, three
Terpene keto-alcohol 1.08mg/g.
Further illustrate, the field pennycress activity extract is that field pennycress is added into powder weight 5 after pulverizing
80% (v/v) ethanol solution again carries out refluxing extraction 3 times, 20 minutes every time, and water bath method in evaporating dish is placed after filtering, is connect
And be dried under reduced pressure to 1.01 times of original volume, add 80% (v/v) ethanol solution containing 2% acetic acid solution and carry out constant volume
Afterwards, the miillpore filter that the speed progress aperture using flow velocity as 0.2m/s is 0.8 μm is filtered, you can is obtained field pennycress activity and is carried
Thing is taken, 3.65mg/g containing volatile oil, scabioside 1.12mg/g.
Further illustrate, the gynostemma pentaphylla activity extract adds the steaming of 5 times of volumes after fresh gynostemma pentaphyllum herb is cleaned
Distilled water carries out homogeneous mashing, is subsequently added into solvent and carries out extraction 3 hours, then carries out the work that micro-filtration molecular cut off is 2000Da
Property material, by trapped fluid rotating speed be 3000r/min seperator in carry out separation 5 minutes, take supernatant be spray-dried after i.e.
Gynostemma pentaphylla activity extract is can obtain, 6.47mg/g containing saponin(e, flavonoids 4.07mg/g in obtained material.
Further illustrate, chaulmoogra herb is added the water of 3 times of volumes in mixer by the chaulmoogra activity extract
Be stirred into slurry, be subsequently added into 5 times of water and stir laggard water-filling bath extraction 60 minutes, be filtrated to get thick liquid, by thick liquid with
0.2m/s flow velocity pours into polyamide chromatography post and often chromatographed, and 3 elutions are carried out using 80% (v/v) ethanol solution, elution
It is 70 DEG C that pressure, which is followed successively by -0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution, is then filtered, be concentrated under reduced pressure, dry,
Chaulmoogra activity extract, 5.34mg/g containing alkaloid, crude fibre 125.36% are obtained after crushing.
Further illustrate, the sorosis activity extract adds 10 times of volumes water after the flower of sorosis is smashed to pieces carries out water-bath
Extraction 30 minutes, the microwave that extraction process frequency of use is 300 hertz carry out assisted extraction, carry out being concentrated under reduced pressure into original after filtering
1.02 times of volume, add 30% (v/v) methanol solution and carry out 3 elutions, the pressure of elution be followed successively by -0.1MPa,
0.1MPa, 0.2MPa, the temperature of elution are 70 DEG C, then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain sorosis activity and carry
Thing is taken, 3.24mg/g containing tannic acid, anthocyanidin 4.27mg/g.
Further illustrate, garlic is put into mixer and is stirred crushing, then used by the garlic activity extract
75% (v/v) ethanol solution is to carry out alcohol extracting at -3 DEG C to stay overnight in temperature, and centrifuge of the rotating speed for 400r/min is used after overnight
It is centrifuged and is filtrated to get thick liquid, normal temperature extracts 3 hours after thick liquid is mixed with protease, is filtrated to get garlic activity and carries
Thing is taken, 31.24mg/g containing allicin；
The water that the rough gentian activity extract adds rough gentian 5 times of volumes is smashed to pieces, is smashed to pieces into after slurry and is using electric-field intensity
25KV/cm high-pressure pulse electric processing, the time of pulse is 10 × 10-6S, the frequency of pulse is 50 hertz, after being handled
Add 10 times 80% (v/v) ethanol solution and carry out ultrasonic extraction 20 minutes using Ultrasound Instrument, the current strength of Ultrasound Instrument is
10A/25W, 0.5 μm of ceramic film component is then poured into 0.25m/s flow velocity and carries out micro-filtration, under 0.2MPa pressure differentials, successively
With molecular cut off 50000Da, 1O000Da membrane module, loop ultrafiltration processing is carried out with cross-current flow, obtains molecular weight ranges
For 15000Da rough gentian activity extract, the glycosides of hardship containing courage 5.32mg/g, chiratin 5.86mg/g.
Further illustrate, the garden burnet activity extract obtains addition 75% (v/v) second after garden burnet stem tuber is crushed
Alcoholic solution, which carries out circulating extraction, obtains extract solution, and it is 2.2 that extract solution is added into salt acid for adjusting pH, then heating water bath while stirring
Continue stirring 8 minutes until occurring filiform in extract solution and starting timing, filter to take filtrate in time, filter residue repeats said extracted step
Rapid 2 times, garden burnet activity extract, 1.91mg/ containing triterpene are can obtain after the filtrate merging of 3 times is dried under reduced pressure, crushed
G, saponins material 1.76mg/g, tannin class material 1.87mg/g；
After Uricularia polytricha is dried the Uricularia polytricha activity extract, add 75% (v/v) ethanol solution of 8 times of volumes to make
With surname extraction is carried out after apparatus,Soxhlet's 2.5 hours, 0.015% chitosan for filtering to take filtrate addition filtrate gross mass is molten
Stirring after liquid filters off filtrate and carries out heating concentration, be freeze-dried to obtain Uricularia polytricha activity extract until filtrate clarification,
147.25mg/g containing Polysaccharide from Auricularia Polytricha Sacc.
Further illustrate, the sweetener is xylitol or oligoisomaltose.
The disease rate of native duck by above method raising is 2.2%.
Experiment one：
Zoopery：
1. experiment material and method
1.1 experimental subjects
1.1.1 sample treatment
Test sample will be that the 1-3 reducing blood lipid compound of preparation is dissolved in distilled water, dilute various concentrations, use during experiment
Physiological saline is by sample preparation into each dosage.
1.1.2 experimental animal and detection environmental condition
Wistar rats are used in experiment, male, healthy cleaning grade, and body weight 180-230g is totally 100, big by Guangxi medical courses in general
Experimental Animal Center is learned to provide.Detection environmental condition, 20-25 DEG C of temperature range, RH range 50-70%, rat are being tried
Adapted to one week in Animal House before testing.
1.2 instruments and reagent
Centrifuge
1.2.2 reagent
Cholesterol, cholate, serum total cholesterol (TC), triglycerides (TG), HDL (HDL-C) measure reagent
Box.Kit is purchased from Nanjing and builds up Bioengineering Research Institute.
1.3 high lipid food formulas
75% basal feed, 4% cholesterol, 10% yolk powder, 10% lard, 1% cholate.
1.4 dosage are grouped and given the test agent gives the time
Experiment sets three dosage groups of the reducing blood lipid compound difference of embodiment 1-3 preparations and one high fat control group, with
5,10,20 times of human body recommended amounts are respectively three dosage groups.It is preventative to the given the test agent time and therapeutic to give given the test agent
Time is 30d.
1.5 experimental procedure
Hyperlipoidemia method-preventative gives given the test agent
Rat feeding basal feed observes 5-10d under experimental situation, and then afterbody takes blood, determines serum total cholesterol
(TC), triglycerides (TG), HDL-C (HDL-C) are horizontal.It is horizontal according to serum total cholesterol (TC), at random
It is divided into 10 groups, every group 10, the given the test agent of various dose is given while high lipid food is given, wherein, high fat control group
(high lipid food+physiological saline), embodiment 1-3 are each 3 dosage of acceptor, are low dose group (high lipid food+tested respectively
Solution 1), middle dose group (high lipid food+test solution 2), high dose group (high lipid food+test solution 3), if basic, normal, high dose
In amount group the concentration of given the test agent solution 1,2,3 be respectively 1.35,2.7,5.4g/kgbw (be respectively equivalent to human body recommended amounts
5,10,20 times), you can obtain A1 low dose groups (high lipid food+embodiment 1 prepare reducing blood lipid compound dissolved matter 1), A2
Middle dose group (reducing blood lipid compound dissolved matter 2 prepared by high lipid food+embodiment 1), A3 high doses group (high lipid food+implementation
Example 1 prepare reducing blood lipid compound dissolved matter 3), B1 low dose groups (high lipid food+embodiment 2 prepare reducing blood lipid compound it is molten
Solve thing 1), B2 middle dose groups (high lipid food+embodiment 2 prepare reducing blood lipid compound dissolved matter 2), B3 high doses group (high fat
Feed+embodiment 2 prepare reducing blood lipid compound dissolved matter 3), C1 low dose groups (high lipid food+embodiment 3 prepare drop blood
Fat compound dissolved matter 1), C2 middle dose groups (high lipid food+embodiment 3 prepare reducing blood lipid compound dissolved matter 2), the high agent of C3
Amount group (reducing blood lipid compound dissolved matter 3 prepared by high lipid food+embodiment 3), gavage amount is 1.0mL/100gbw.Daily one
It is secondary, continuous 30 days, weigh once weekly, and adjust gavage amount according to this.Experiment to taken a blood sample respectively at the 28th day measure serum TC,
TG, HDL-C content, each group Rat Fast 16h before blood sampling.Serum TC, the measure of TG and HDL-C contents use kit.
1.6 experimental datas count
Data summarization and analysis are carried out using spss19.0 and sigmaplot11.0 softwares.
2 experimental results
Influence of the 2.1 auxiliary reducing blood lipid reducing blood lipid compounds to rat body weight
Table 1 aids in influence of the reducing blood lipid reducing blood lipid compound to rat body weight
From table 1, rat is not significantly different to (0d) before tested material, each group body weight.To the tested material regular period
After (7d, 14d, 21d, 28d), compared with high fat control group, remaining each group body weight does not occur significant difference (p ＞ 0.05).Table
The bright auxiliary reducing blood lipid reducing blood lipid compound does not have harmful effect to rat growthing development.
Influence of the 2.2 auxiliary reducing blood lipid reducing blood lipid compounds to rat blood serum T-CHOL (TC) content
Table 2 aids in influence of the reducing blood lipid reducing blood lipid compound to rat blood serum T-CHOL (TC) content
From table 2, rat is given before tested material (0d), and each experimental group serum total cholesterol (TC) content compares with high fat
Group compares that there was no significant difference (p ＞ 0.05).After the tested material regular period (7d, 14d, 21d, 28d), each period high fat control
Compared with to before tested material, serum TC rise, difference has pole conspicuousness (p ＜ 0.01), shows that high TC models are successfully established group.
Compared with high fat control group, remaining each dosage group significantly reduces (p ＜ 0.05).Show the auxiliary reducing blood lipid reducing blood lipid compound
Have the function that to reduce serum total cholesterol (TC) content.
Influence of the 2.3 auxiliary reducing blood lipid reducing blood lipid compounds to rat blood serum triglycerides (TG) content
Table 3 aids in influence of the reducing blood lipid reducing blood lipid compound to rat blood serum triglycerides (TG) content
From table 3, rat is given before tested material (0d), and each experimental group serum levels of triglyceride (TG) content compares with high fat
Group compares that there was no significant difference (p ＞ 0.05).After the tested material regular period (7d, 14d, 21d, 28d), each period high fat control
Compared with to before tested material, serum TG rise, difference has pole conspicuousness (p ＜ 0.01), shows that high TG models are successfully established group.
Compared with high fat control group, remaining each dosage group significantly reduces (p ＜ 0.05).Show the auxiliary reducing blood lipid reducing blood lipid compound
Have the function that to reduce serum levels of triglyceride (TG) content.
Influence of the 2.4 auxiliary reducing blood lipid reducing blood lipid compounds to rat HDL (HDL-C) content
Table 4 aids in influence of the reducing blood lipid reducing blood lipid compound to rat HDL (HDL-C) content
From table 4, give before tested material (0d), each experimental group HDL (HDL) content and high fat control group
Compare that there was no significant difference (p ＞ 0.05).After the tested material regular period (7d, 14d, 21d, 28d), each period high fat control group
Compared with to before tested material, HDL (HDL) content rise, difference has pole conspicuousness (p ＜ 0.01), shows height
HDL-C models are successfully established.Compared with high fat control group, remaining each dosage group significantly reduces (p ＜ 0.05).Illustrate to show this
Auxiliary reducing blood lipid reducing blood lipid compound has the function that to increase HDL (HDL) content.
According to《Function of health food assessment process and the method for inspection》Criterion to health-care food for assisting blood fat lowering
(results of animal judgement) is to auxiliary lipid-lowering function result judgement：In serum total cholesterol, triglycerides, high density lipoprotein level
Serum total cholesterol and triglycerides binomial index are positive in three Indexs measures of white cholesterol, can determine that the given the test agent aids in
Hypolipemic function results of animal is positive.
Described above is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, the equal change completed or modification change under the technical spirit suggested by all present invention, all should belong to
Cover the scope of the claims in the present invention.
Claims (10)
1. a kind of cultural method of native duck, it is characterised in that operate in accordance with the following steps：
For the native duck in 2-3 week old, the feed for configuring to obtain according to the raw material of following mass percentage is fed, 3 times/
My god, and 100-300g/ every time, and by its free choice feeding to all you can eat；
The composition of the feed is as follows：Oyster shell whiting 45-55%, reducing blood lipid compound 5-10%, shrimp powder 8-15%, soybean residue
10-15%, fermentation purple napier grass 5-10%, fish meal 3-5%, salt 1-3%, calcium monohydrogen phosphate 2-4%；
The reducing blood lipid compound is made up according to percent by weight of following raw material：Folum Ilicis extract 10-18%, rhizoma alismatis carry
Take thing 10-20%, Herba Patriniae extract 8-15%, strong wind seed extract 8-15%, sorosis extract 5-10%, garlic P.E
5-10%, Radix Gentianae extract 5-10%, Herb Gynostemmae Pentaphylli extract 3-10%, Radix Sangusorbae extract 5-12%, Uricularia polytricha extract 5-
10%；
The preparation method of the reducing blood lipid compound is：Gross weight is added after above-mentioned extract is mixed according to percent by weight
After the 10-30% water of amount is mixed into dope, sweetener, hydroxymethyl cellulose, sweetener are added：Hydroxymethyl cellulose：It is sticky
Thing is 0.15-0.23 according to ratio of weight and number:0.55-0.65:After 10-12 stirs, it is put into drying machine and is dried, dries
While the use of power is 50-80W, frequency be that 30-50 hertz ultrasonic waves are modified processing, you can obtain powdery reducing blood lipid and mix
Close material.
2. the cultural method of native duck according to claim 1, it is characterised in that the Ilex Latifolia Thunb activity extract will be fresh
Ilex Latifolia Thunb herb carries out infusion 30-40 minutes after adding the water of 5-10 times of volume, is filtered while hot, takes filtrate, by filtrate
Carry out stopping heating after heating is concentrated into 1.02 times of original volume, obtain medicinal extract and be subsequently added into cellulase, 70-80% (v/v)
Ethanol solution carries out surname extraction 1-2 hours, filtrate is dried under reduced pressure after filtering, crush after to can obtain Ilex Latifolia Thunb active
Extract, it is stand-by；The medicinal extract：Cellulase：The weight ratio of ethanol solution is 3-4:1-2:18-22.
3. the cultural method of native duck according to claim 1, it is characterised in that the rhizoma alismatis activity extract is damp by 1kg
Rush down after being crushed, put into supercritical CO2In extraction equipment, 3-4 hours are extracted, collect extract solution, filter residue is added into filter residue matter
70-80% (v/v) ethanol solution of 3-5 times of amount, carry out refluxing extraction 20-30 minutes, the temperature of backflow is at 65-85 DEG C, is connect
And 0.3 μm of ceramic film component progress micro-filtration is poured into 0.1-0.2m/s flow velocity, under 0.1-0.2MPa pressure differentials, use cut successively
Molecular weight 10000Da, 5000Da membrane module are stayed, loop ultrafiltration processing is carried out with cross-current flow, obtaining molecular weight ranges is
5000Da-6000Da rhizoma alismatis activity extract.
4. the cultural method of native duck according to claim 1, it is characterised in that the field pennycress activity extract is to lose
60-80% (v/v) ethanol solution that sauce grass adds 3-5 times of powder weight after pulverizing carries out refluxing extraction 3 times, every time
20 minutes, water bath method in evaporating dish is placed after filtering, is then dried under reduced pressure to 1.01 times of original volume, addition and contains 1-
After 70-80% (v/v) ethanol solution of 2% acetic acid solution carries out constant volume, the speed using flow velocity as 0.1-0.2m/s carries out aperture
Filtered for 0.5-0.8 μm of miillpore filter, you can obtain field pennycress activity extract.
5. the cultural method of native duck according to claim 1, it is characterised in that the gynostemma pentaphylla activity extract will be fresh
The distilled water that 3-5 times of volume is added after gynostemma pentaphyllum herb removal of impurities carries out homogeneous mashing, be subsequently added into solvent carry out extracting 1-3 it is small
When, the active material that micro-filtration molecular cut off is 1000-2000Da is then carried out, in rotating speed is 2000-3000r/ by trapped fluid
Carry out separating 3-5 minutes in min seperator, take supernatant to can obtain gynostemma pentaphylla activity extract after being spray-dried.
6. the cultural method of native duck according to claim 1, it is characterised in that the chaulmoogra activity extract is by strong wind
The water that sub- herb adds 2-3 times of volume is stirred into slurry in mixer, is subsequently added into 3-5 times of water and stirs laggard water-filling
Bath extraction 30-60 minutes, thick liquid is filtrated to get, thick liquid is poured into the frequent layer of polyamide chromatography post with 0.1-0.2m/s flow velocity
Analysis, 3 elutions are carried out using 70-80% (v/v) ethanol solution, the pressure of elution be followed successively by -0.1MPa, 0.1MPa, 0.2MPa,
The temperature of elution be 60-70 DEG C, then filtered, be concentrated under reduced pressure, dry, crush after obtain chaulmoogra activity extract.
7. the cultural method of native duck according to claim 1, it is characterised in that the sorosis activity extract is by sorosis
Flower adds 5-10 times of volume water after smashing to pieces carries out water-bath extraction 20-30 minutes, and extraction process frequency of use is 200-300 hertz
Microwave carry out assisted extraction, be concentrated under reduced pressure into 1.02 times of original volume after filtering, add 20-30% (v/v) first
Alcoholic solution carries out 3 elutions, and it is 60-70 DEG C that the pressure of elution, which is followed successively by -0.1MPa, 0.1MPa, 0.2MPa, the temperature of elution,
Then carry out three layers of filtered through gauze, be concentrated under reduced pressure after obtain sorosis activity extract.
8. the cultural method of native duck according to claim 1, it is characterised in that the garlic activity extract puts garlic
Enter mixer and be stirred crushing, the use of 65-75% (v/v) ethanol solutions is then that alcohol extracting is carried out at -5--3 DEG C in temperature
At night, use rotating speed to be centrifuged for 300-400r/min centrifuge after overnight and be filtrated to get thick liquid, by thick liquid and albumen
Normal temperature extraction 1-3 hours, are filtrated to get garlic activity extract after enzyme mixing；
The water that the rough gentian activity extract adds rough gentian 2-5 times of volume is smashed to pieces, is smashed to pieces into after slurry and is using electric-field intensity
25KV/cm high-pressure pulse electric processing, the time of pulse is 5 × 10-6-10×10-6S, the frequency of pulse is 30-50 hertz,
5-10 times 70-80% (v/v) ethanol solution is added after being handled and carries out ultrasonic extraction 10-20 minutes using Ultrasound Instrument, is surpassed
The current strength of sound instrument is 5A/25W-10A/25W, and then pouring into 0.5 μm of ceramic film component with 0.1-0.25m/s flow velocity is carried out
Micro-filtration, under 0.1-0.2MPa pressure differentials, successively with molecular cut off 50000Da, 1O000Da membrane module, with cross-current flow
Loop ultrafiltration processing is carried out, obtains the rough gentian activity extract that molecular weight ranges are 10000Da-15000Da.
9. the cultural method of native duck according to claim 1, it is characterised in that the garden burnet activity extract is by garden burnet block
Stem obtained after being crushed add 70-80% (v/v) ethanol solution carry out circulate extraction obtain extract solution, extract solution is added into salt
Acid for adjusting pH is 2.0-3.0, and then heating water bath continues to stir until filiform occur in extract solution and starting timing while stirring
5-10 minutes, filtrate is filtered to take in time, filter residue repeats said extracted step 2 time, the filtrate merging of 3 times is dried under reduced pressure,
Garden burnet activity extract is can obtain after crushing；
After Uricularia polytricha is dried the Uricularia polytricha activity extract, 70-80% (v/v) ethanol for adding 5-10 times of volume is molten
Liquid carries out surname extraction 2-3 hours after using apparatus,Soxhlet's, filters to take the 0.01-0.02%'s of filtrate addition filtrate gross mass
Stirring after chitosan solution filters off filtrate and carries out heating concentration until filtrate clarification, is freeze-dried to obtain Uricularia polytricha work
Property extract.
10. the cultural method of native duck according to claim 1, it is characterised in that the sweetener is xylitol or oligomeric
Isomaltose.
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| CN104940549A (en) * | 2015-06-23 | 2015-09-30 | 佛山科学技术学院 | Chinese herbal recipe for lowering lipid of large meat ducks |
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2017
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| JPS63313535A (en) * | 1987-06-16 | 1988-12-21 | Masayuki Otsuki | System for activating physical constitution of domestic animal |
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