CN108051460A - A kind of Micro-CT scanning sample preparation methods of stationery substance - Google Patents
A kind of Micro-CT scanning sample preparation methods of stationery substance Download PDFInfo
- Publication number
- CN108051460A CN108051460A CN201711281078.5A CN201711281078A CN108051460A CN 108051460 A CN108051460 A CN 108051460A CN 201711281078 A CN201711281078 A CN 201711281078A CN 108051460 A CN108051460 A CN 108051460A
- Authority
- CN
- China
- Prior art keywords
- sample
- sealed membrane
- parafilm
- paraffinic base
- stationery
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N23/00—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00
- G01N23/02—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material
- G01N23/04—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material and forming images of the material
- G01N23/046—Investigating or analysing materials by the use of wave or particle radiation, e.g. X-rays or neutrons, not covered by groups G01N3/00 – G01N17/00, G01N21/00 or G01N22/00 by transmitting the radiation through the material and forming images of the material using tomography, e.g. computed tomography [CT]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
Landscapes
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Theoretical Computer Science (AREA)
- Engineering & Computer Science (AREA)
- Radiology & Medical Imaging (AREA)
- Pulmonology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides a kind of Micro-CT scanning sample preparation methods of stationery substance, and sample using paraffinic base sealed membrane Parafilm is wrapped up, is then fixed to paraffin on specimen holder by feature this method.The yielding sample of stationery is clamped by two layers of paraffinic base sealed membrane Parafilm, a kind of composites sandwich structures is formed, makes its intensity and rigidity reinforced, can be uprightly on specimen holder, and improve the ability for resisting the small external force such as air flow.
Description
Technical field
The present invention relates to a kind of preparation methods, and in particular to a kind of Micro-CT scanning sample preparation methods of stationery substance.
Background technology
Micro-CT scanning（Micro-Computed Tomography）, it is also referred to as X-ray microfault photography.Refer to space point
Resolution can reach the CT of lum or so.The main composition of Micro-CT scanning includes X radiographic sources, specimen rotating holder and high-resolution and visits
Survey three parts of device.X-ray source and detector are fixed, and sample rotates between X radiographic sources and detector, are connected by X radiographic sources
The continuous X beams for generating taper penetrate sample to be tested, are imaged on X-ray detector.By rate at an angle to sample
Product are rotated, and obtain x-ray perspective view of the sample in different angle.By corresponding computer software, by each angle
Projected image be reconstructed, be reduced into the analyzable 3D rendering in computer.Sample is nearer from light source, and resolution ratio is higher, institute
With in order to reach higher resolution ratio, the distance of sample and light source is very near, such as 2211 type Micro-CT scanning of Brooker, works as resolution ratio
For 100nm when, the distance at sample center to light source is about 0.5mm.At this point, in order to which sample is made not touch the beryllium of light source when rotated
Window, it is necessary to assure the diameter of sample is less than 1mm, while in order to coordinate high-resolution, the rotary step of sample also must very little, i.e.,
Rotary speed is very slow, and sweep time is very long, when the sample needs 15 of usually one 100nm resolution ratio of scanning are small or so, even 1
The scanning of μm resolution ratio be also required to 3 it is small when or so.
In order to reach preferable Micro-CT scanning test effect, sample is in scanning process in addition to being rotated with sample stage, it is impossible to
There are other any variations, otherwise cannot reconstruct the pattern or institutional framework of sample, cause test crash.Due to paper etc. compared with
Soft sample is by external force or environmental condition（Temperature, humidity, air flow etc.）Deformation is very easy to after variation, if without place
Not only thin but also narrow small paper slip, is directly put on sample stage and is scanned for a long time, sample is bound to deform, cannot be well by reason
Result.So being badly in need of finding a kind of sample preparation methods, make it that stationery soft sample can be kept indeformable, and do not influence
Ray penetrates effect to sample.
The content of the invention
It is an object of the invention to overcome the shortage of prior art, a kind of Micro-CT scanning sample preparation side of stationery substance is provided
Method, the sample prepared by this method is indeformable during the test, and the quality reconstruction tested is good, sample nondestructive after test
Wound.
Detailed description of the invention
The present invention provides a kind of Micro-CT scanning sample preparation methods of stationery substance, and this method utilizes paraffinic base sealed membrane Parafilm
Sample is wrapped up, is then fixed to viscous paraffin on specimen holder, is as follows：
1. being tested according to Micro-CT scanning needs, stationery material sample is processed into suitable size；
2. take the paraffinic base sealed membrane Parafilm of suitable size, along center line doubling after open again；
3. sample is put on paraffinic base sealed membrane Parafilm, one side of sample is made to be overlapped with sealed membrane center line；
4. by paraffinic base sealed membrane Parafilm doublings, sample is clipped among two layers of paraffinic base sealed membrane Parafilm, gently
Pressure makes sample be contacted as far as possible with paraffinic base sealed membrane Parafilm；
5. paraffinic base sealed membrane Parafilm is pressed along sample edge outside vertical with the knife spine of wallpaper blade（Center line one side can be with
It does not press）, combine two layers of sealed membrane at the place of being squeezed；
6. extra paraffinic base sealed membrane Parafilm is cut off along on the outside of knife spine impression with wallpaper blade；
7. sample made from step 6 is fixed with paraffin（Bonding）Onto specimen holder.
Advantageous effect
Paraffinic base sealed membrane Parafilm used in the present invention is a kind of moldable, good toughness special Self-enclosing film production
Product, can fast and effeciently sealing experiment room various vessel, can be fitted closely with various substances；Possesses the damp proof work(of waterproof
Can, the volatilization and pollution of laboratory sample had not only been can effectively stop, but also can effectively protect anhydrous substances；Can be repetitively folded makes
With being wrapped on sharp shape object will not be torn.The present invention clamps paper using two layers of paraffinic base sealed membrane Parafilm
The yielding sample of class, fitted to paraffinic base sealed membrane Parafilm due to stationery sample together with, form a kind of interlayer structure
Composite material makes its intensity and rigidity reinforced, can be uprightly on specimen holder, and improves that resist air flow etc. small outer
The ability of power；Since sample is sealed in sealed membrane, influence of the external condition to sample tissue structure is also prevented significantly, makes sample
Product will not deform due to structure change；Paraffinic base sealed membrane Parafilm mainly waits light elements to form by hydrocarbon, and density is smaller, X
Radiation absorption easily penetrates, and does not influence the test of stationery sample, apparent with the comparison of stationery sample in the result, passes through image procossing
Easily filter out；Paraffinic base sealed membrane Parafilm can be well bonded with stationery substance, but its viscosity is poor, not to sample
Internal penetration substance, is completed, and can easily remove it, and keeps sample intact.
Specific embodiment
The present invention is further elaborated with reference to embodiment, but institute's protection domain of the present invention is without being limited thereto.
The embodiment of the present invention provides a kind of Micro-CT scanning of ordinary filter paper（700nm resolution ratio）Sample preparation methods, this method
Sample is wrapped up using U.S.'s Parafilm M sealed membranes, is then fixed to paraffin on specimen holder, specific steps are such as
Under：
1. testing needs according to a kind of ordinary filter paper 700nm resolution ratio Micro-CT scanning, filter paper sample is processed into 4mm*8mm sizes；
2. intercept about 40mm*40mm sizes Parafilm M sealed membranes, along center line doubling after open again；
3. filter paper sample is put on sealed membrane, one side of sample is made to be overlapped with sealed membrane center line；
4. by sealed membrane doubling, filter paper sample is clipped among two layers of sealed membrane, gentle pressure, sample is made to be connect as far as possible with sealed membrane
It touches；
5. sealed membrane is pressed along sample edge outside vertical with the knife spine of wallpaper blade（It does not press center line one side）, make the place that is squeezed
Two layers of sealed membrane combines.
6. extra sealed membrane is cut off along on the outside of knife spine impression with wallpaper blade.
7. use viscous paraffin（Correction wax）Sample made from step 6 is fixed（Bonding）Onto specimen holder.
Above example is merely to illustrate the present invention and is used rather than limitation of the present invention, person skilled in the relevant technique,
In the case of not departing from the spirit and scope of the present invention, various conversion or modification, therefore all equivalent technologies can also be made
Scheme should also belong to scope of the invention, should be limited by each claim.
Claims (2)
- A kind of 1. Micro-CT scanning sample preparation methods of stationery substance, it is characterised in that the Micro-CT scanning sample preparation of the stationery substance Method is wrapped up sample using paraffinic base sealed membrane Parafilm, is then fixed to paraffin on specimen holder.
- 2. the Micro-CT scanning sample preparation methods of stationery substance as described in claim 1, which is characterized in that be as follows：Step 1. tests needs according to Micro-CT scanning, and stationery material sample is processed into suitable size；Step 2. takes the paraffinic base sealed membrane Parafilm of suitable size, along center line doubling after open again；Sample is put on paraffinic base sealed membrane Parafilm by step 3., and one side of sample is made to be overlapped with sealed membrane center line；Sample is clipped to paraffinic base sealed membrane Parafilm doublings among two layers of paraffinic base sealed membrane Parafilm by step 4., Gentle pressure makes sample be contacted as far as possible with paraffinic base sealed membrane Parafilm；Step 5. presses paraffinic base sealed membrane Parafilm with the knife spine of wallpaper blade along sample edge outside vertical（Center line one Side can not press）, combine two layers of sealed membrane at the place of being squeezed；Step 6. is cut off extra paraffinic base sealed membrane Parafilm along on the outside of knife spine impression with wallpaper blade；Step 7. is fixed sample made from step 6 with paraffin（Bonding）Onto specimen holder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711281078.5A CN108051460A (en) | 2017-12-07 | 2017-12-07 | A kind of Micro-CT scanning sample preparation methods of stationery substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711281078.5A CN108051460A (en) | 2017-12-07 | 2017-12-07 | A kind of Micro-CT scanning sample preparation methods of stationery substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108051460A true CN108051460A (en) | 2018-05-18 |
Family
ID=62123341
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711281078.5A Pending CN108051460A (en) | 2017-12-07 | 2017-12-07 | A kind of Micro-CT scanning sample preparation methods of stationery substance |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108051460A (en) |
Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994024563A1 (en) * | 1993-04-13 | 1994-10-27 | Serex, Inc. | An integrated packaging-holder device for immunochromatographic assays in flow-through or dipstick formats |
| CN1217060A (en) * | 1996-05-02 | 1999-05-19 | 大纳宝株式会社 | Immunochromatographic assay device |
| CN1266186A (en) * | 1999-02-16 | 2000-09-13 | 株式会社松本生命科学研究所 | Medical testing method and sampling apparatus thereof |
| CN1464294A (en) * | 2002-06-07 | 2003-12-31 | 清华大学 | Process of transmission electron microscope specimen preparation for easy damp-affecting denaturization crystal thin film |
| CN102375054A (en) * | 2010-08-06 | 2012-03-14 | 艾博生物医药(杭州)有限公司 | Detection device comprising immune test strips and production method of detection device |
| CN102721580A (en) * | 2012-07-11 | 2012-10-10 | 山东省分析测试中心 | Multifunctional automatic acquisition and sample preparation system for airborne dust |
| CN102879583A (en) * | 2012-09-21 | 2013-01-16 | 田伏洲 | Test card for rapidly diagnosing pancreatic trauma and acute pancreatitis and preparation method thereof |
| CN202735339U (en) * | 2012-08-03 | 2013-02-13 | 云南昊戌生物科技有限公司 | Plastic encapsulated reagent strip |
| CN104807840A (en) * | 2015-04-28 | 2015-07-29 | 苏州新材料研究所有限公司 | Sample fixing device for superconducting strip XRD texture measurement |
| CN205450000U (en) * | 2016-02-25 | 2016-08-10 | 杭州冰桶生物科技有限公司 | Pregnant excellent device is tested to electron |
| CN205484368U (en) * | 2016-02-24 | 2016-08-17 | 杭州泰熙生物技术有限公司 | Clenobuterol hydrochloride short -term test cup |
| JP6154173B2 (en) * | 2013-04-03 | 2017-06-28 | 株式会社ナノテム | Vacuum suction device and suction plate |
-
2017
- 2017-12-07 CN CN201711281078.5A patent/CN108051460A/en active Pending
Patent Citations (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994024563A1 (en) * | 1993-04-13 | 1994-10-27 | Serex, Inc. | An integrated packaging-holder device for immunochromatographic assays in flow-through or dipstick formats |
| CN1217060A (en) * | 1996-05-02 | 1999-05-19 | 大纳宝株式会社 | Immunochromatographic assay device |
| CN1266186A (en) * | 1999-02-16 | 2000-09-13 | 株式会社松本生命科学研究所 | Medical testing method and sampling apparatus thereof |
| CN1464294A (en) * | 2002-06-07 | 2003-12-31 | 清华大学 | Process of transmission electron microscope specimen preparation for easy damp-affecting denaturization crystal thin film |
| CN102375054A (en) * | 2010-08-06 | 2012-03-14 | 艾博生物医药(杭州)有限公司 | Detection device comprising immune test strips and production method of detection device |
| CN102721580A (en) * | 2012-07-11 | 2012-10-10 | 山东省分析测试中心 | Multifunctional automatic acquisition and sample preparation system for airborne dust |
| CN202735339U (en) * | 2012-08-03 | 2013-02-13 | 云南昊戌生物科技有限公司 | Plastic encapsulated reagent strip |
| CN102879583A (en) * | 2012-09-21 | 2013-01-16 | 田伏洲 | Test card for rapidly diagnosing pancreatic trauma and acute pancreatitis and preparation method thereof |
| JP6154173B2 (en) * | 2013-04-03 | 2017-06-28 | 株式会社ナノテム | Vacuum suction device and suction plate |
| CN104807840A (en) * | 2015-04-28 | 2015-07-29 | 苏州新材料研究所有限公司 | Sample fixing device for superconducting strip XRD texture measurement |
| CN205484368U (en) * | 2016-02-24 | 2016-08-17 | 杭州泰熙生物技术有限公司 | Clenobuterol hydrochloride short -term test cup |
| CN205450000U (en) * | 2016-02-25 | 2016-08-10 | 杭州冰桶生物科技有限公司 | Pregnant excellent device is tested to electron |
Non-Patent Citations (1)
| Title |
|---|
| 罗国华等: "CT扫描技术在纸张结构研究中的应用", 《造纸科学与技术》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106053168B (en) | Concrete based on 3D printing technique carefully sees the method for visualizing of three-phase structure | |
| JP4675869B2 (en) | Imaging system | |
| JP4711759B2 (en) | X-ray inspection equipment | |
| WO2007056922A1 (en) | System and method for reconstructing an image by rectilinear trajectory scanning | |
| IT9020430A1 (en) | TRANSLATION AND ROTATION ANALYSIS METHOD FOR X-RAY IMAGE REPRESENTATION | |
| Manahiloh et al. | Determining the soil water characteristic curve and interfacial contact angle from microstructural analysis of X-ray CT images | |
| McKinley et al. | Analysis of adhesive penetration into wood using nano-X-ray computed tomography | |
| CN108051460A (en) | A kind of Micro-CT scanning sample preparation methods of stationery substance | |
| CN103876770A (en) | Small animal multispectral fluorescence tomography system excited by narrow-beam X-ray | |
| JPS61269046A (en) | Ct apparatus | |
| US9063065B2 (en) | Sample analysis | |
| CN104132950B (en) | CL scanning means projection rotating center scaling method based on original projection information | |
| JP4752468B2 (en) | Cross-sectional image reconstruction apparatus and X-ray imaging apparatus using the same | |
| JP7306528B2 (en) | Control device, display method and inspection method | |
| CN104165895B (en) | The formation method of the 3D imaging system of industry CT | |
| CN105678823B (en) | A kind of multi-joint dress D fan computer tomography method | |
| Vaziri et al. | Nano X-ray tomography analysis of the cell-wall density of welded beech joints | |
| JP6708857B2 (en) | X-ray inspection device | |
| IT1021392B (en) | DEVICE FOR DETECTION OF ANGULAR POSITIONS OF AN ULTRASONIC BEAM INTENDED IN PARTICULAR FOR THE NON-DESTRUCTIVE EXAMINATION OF MATERIALS AND PROCEDURE FOR SUCH DETECTION | |
| ITVR20090146A1 (en) | "METHOD FOR SCANNING THE INTERNAL QUALITY OF WOOD ELEMENTS HAVING A DIRECTION OF PREVALENT DEVELOPMENT, AS TRUNKS OR TABLES" | |
| CN101424869A (en) | X ray double-wire array three-dimensional imaging method | |
| CN108051459A (en) | A kind of Micro-CT scanning Multi-example test processing method | |
| WO2007145109A1 (en) | Ct apparatus having x-ray irradiating direction and detector orientation set in arbitrary directions, and three-dimensional image reconstituting method and program for the apparatus | |
| JP4078846B2 (en) | Tomography equipment | |
| CN104306013B (en) | A kind of method of adjustment of CT system neutral array detector position |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |