CN108051412A - A kind of method of tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid - Google Patents
A kind of method of tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid Download PDFInfo
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- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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Abstract
The present invention provides a kind of method of tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid, including：(1) the identical tetranitro aluminium phthalocyanine standard solution of more parts of concentration is prepared, the fluorescence intensity of bioassay standard solution is F0, the ascorbic acid solution of various concentration is added in into each part standard solution, the fluorescence intensity for measuring each part mixed solution is Fi, establish F0‑FiAnd CiBetween linear relationship, wherein CiFor the concentration of ascorbic acid solution；(2) standard solution in step (1) is taken, ascorbic acid solution to be measured is added in into the standard solution, the fluorescence intensity for measuring mixed solution is Fx, the linear relationship according to step (1) determines ascorbic acid content in solution to be measured.Since ascorbic acid has fluorescence quenching to tetranitro aluminium phthalocyanine, linear relationship between fluorescence intensity difference and ascorbic acid concentrations is directly established by the fluorescence intensity for detecting tetranitro aluminium phthalocyanine in solution, the method for the present invention has the characteristics that easy to operate, analysis time is short and detection sensitivity is high.
Description
Technical field
The invention belongs to technical field of chemical detection, and in particular to a kind of tetranitro aluminium phthalocyanine near-infrared fluorescent detection is anti-bad
The method of hematic acid.
Background technology
Ascorbic acid (Ascorbic acid, abbreviation AA) is a kind of important substance for maintaining health.Needed by human body will
Most of ascorbic acid by water fruits and vegetables and food supply.Human body long-term lacking ascorbic acid can obtain scurvy, arthritis
Bitterly, bleeding gums, inflammation, infection, body bone easy damaged and being not easy such as heal at the diseases, and Excess free enthalpy cannot not only resist disease
Disease, but also the service life may be shortened.This is because ascorbic acid can destroy DNA free radicals, this destruction can cause early ageing and
Cancer.The quantitative analysis of ascorbic acid is quite important in food, medicine and other fields, and therefore, the quantitative analysis of ascorbic acid just has
There is important meaning.
Measuring the method for ascorbic acid at present mainly has electrochemical detection method, but this kind of method is usually because Vitamin C
Sour oxidizing potential and the oxidizing potential of dopamine and uric acid is close and causes selectivity bad.In addition, there is chemistry
The detection methods such as luminous, high performance liquid chromatography, Capillary Electrophoresis, flow injection and colorimetric, still, since these methods are in sample
Deficiency in terms of the preparation of product and analysis cost limits the application of these methods.These methods are cumbersome, intermediate with pre-treatment
It to convert repeatedly and measure the deficiencies of medium, analysis time are longer and sensitivity is not high.Therefore, easy to operate, analysis time is established
Short and high sensitivity ascorbic acid new detecting method is just particularly important.
The content of the invention
It is an object of the invention to overcome above-mentioned technical deficiency, it is anti-to provide a kind of tetranitro aluminium phthalocyanine near-infrared fluorescent detection
The technical issues of method of bad hematic acid, solution is complicated for operation in the prior art, analysis time is long and sensitivity is not high.
To reach above-mentioned technical purpose, technical scheme provides a kind of tetranitro aluminium phthalocyanine near-infrared fluorescent detection
The method of ascorbic acid, comprises the following steps：
S1. linear relationship is established：The identical tetranitro aluminium phthalocyanine standard solution of more parts of concentration is prepared, bioassay standard solution
Fluorescence intensity is F0, the ascorbic acid solution of various concentration is added in into each part standard solution, measures the glimmering of each part mixed solution
Luminous intensity is Fi, establish F0-FiAnd CiBetween linear relationship, wherein CiFor the concentration of ascorbic acid solution；
S2. detect：The standard solution in step S1 is taken, ascorbic acid solution to be measured is added in into the standard solution, is surveyed
The fluorescence intensity for determining mixed solution is Fx, the linear relationship according to step S1 determines ascorbic acid content in solution to be measured.
Compared with prior art, beneficial effects of the present invention include：
The technical program is fluorescence probe detection ascorbic acid content with tetranitro aluminium phthalocyanine, due to tetranitro aluminium phthalocyanine
Fluorescence emission spectrum is near infrared region, it is possible to reduce interference and raising sensitivity in detection process；The technical program utilizes anti-
Bad hematic acid establishes the new method of detection ascorbic acid to the fluorescence quenching of tetranitro aluminium phthalocyanine, tetranitro aluminium phthalocyanine it is glimmering
Luminous intensity gradually weakens with the increase for adding in ascorbic acid amount, straight by the fluorescence intensity for detecting tetranitro aluminium phthalocyanine in solution
Connect establish fluorescence intensity difference ascorbic acid concentrations between linear relationship, further according to fluorescence intensity difference and ascorbic acid concentrations it
Between relation, determine the ascorbic acid content in sample to be tested, need not be converted in entire detection process measure medium, operation
Simply, analysis time is short and detection sensitivity is high.
Description of the drawings
Fig. 1 is the fluorescence emission spectrogram of compound of tetranitro aluminium phthalocyanine of the present invention；
Fig. 2 is the uv-visible absorption spectra of tetranitro aluminum phthalocyanine of the present invention；
Fig. 3 is influence figure of the tetranitro aluminium phthalocyanine concentration to fluorescence intensity in the embodiment of the present invention 1；
Fig. 4 is influence figure of the time of measuring to fluorescence intensity in the embodiment of the present invention 4；
Fig. 5 is the linear relationship chart between the fluorescence intensity difference and ascorbic acid concentrations established in the embodiment of the present invention 5.
Specific embodiment
The present embodiment provides a kind of method of tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid, including following step
Suddenly：
(1) linear relationship is established：The tetranitro aluminium phthalocyanine of solid-like is prepared into 1.0 × 10-3The tetranitro aluminium phthalein of g/ml
Cyanines solution, it is 1.0 × 10 that 15~45 μ l concentration are added in colorimetric cylinder-3The tetranitro aluminium phthalocyanine solution and pH of g/ml for 5.5~
9.5 B-R buffer solution 0.25ml, and be settled to 5.0mL with DMF, that is, it is 3 × 10 that concentration, which is made,-6~9 × 10-6The four of g/ml
Nitro aluminium phthalocyanine standard solution, preferably, selecting, pH is 7.0 and tetranitro aluminium phthalocyanine concentration is 6 × 10-6The standard of g/ml is molten
Liquid measures fluorescence intensity；With pipette the above-mentioned tetranitro aluminium phthalocyanine standard solution of 2.00ml is taken to be started in luminoscope cuvette
Luminoscope, temperature are set as 21~45 DEG C, heat up and keep the temperature 3min, using 620nm as excitation wavelength, measure the solution in 700nm
Fluorescence intensity at wavelength, is denoted as F0；Tetranitro aluminium phthalocyanine concentration and solution ph is kept to immobilize, contains tetranitro to above-mentioned
The fluorescence intensity of mixed solution, institute are measured in the cuvette of aluminium phthalocyanine standard solution after the ascorbic acid solution of addition various concentration
State ascorbic acid concentrations CiFor 2.5 × 10-8~3.0 × 10-7G/ml is 21~45 DEG C in temperature, when the time is 1~11min, makees
To be preferred, temperature is 30 DEG C when measuring fluorescence intensity, minute 3min；Using 620nm as excitation wavelength, mixed solution is measured
The fluorescence intensity at 700nm wavelength, is denoted as Fi, with F0-FiFor ordinate, using AA concentration as abscissa, standard curve is drawn,
Establish F0-FiAnd CiBetween linear relationship.
The preparation method of above-mentioned tetranitro aluminium phthalocyanine is specially：Weigh 4- nitrophthalimides, the 5.0g of 1.9g
Urea and the mixing of 0.02g~0.03g ammonium molybdates, are put into after grinding in the beaker of a 250ml, and the sealing of beaker mouth and water-bath are added
Heat fusing, after complete melting, the anhydrous Aluminum chloride that 0.3g is ground is added in fusant, and is stirred evenly with glass bar, permanent
Temperature heating mixture, it can be seen that the solid for having bluish violet occurs, and constantly blisters.After foam expansion phenomenon stops completely,
It cools down and fragmentation solid, above-mentioned solid is each with the dilute hydrochloric acid solution and 150 μm of ol/l sodium hydroxide solutions of 150 μm of ol/l respectively
After slightly boiling 1h, product is all washed till neutrality by filtering, every time filtering with water, and product is put into the baking oven that temperature is 60 DEG C and is dried,
The tetranitro aluminium phthalocyanine of solid-like is made.
The selection method of above-mentioned fluoroscopic examination wavelength is specially：It is 6.0 × 10 to concentration-6The tetranitro aluminium phthalocyanine of g/ml is molten
Concentration is separately added into liquid as 1.0 × 10-8~1.0 × 10-6The ascorbic acid solution of g/ml, obtained fluorescence emission spectrogram of compound
With the uv-visible absorption spectra of tetranitro aluminium phthalocyanine, Fig. 1 and Fig. 2 are seen.As seen from Figure 1, the peak value of every curve corresponds to
Wavelength be all located at 700nm；By Fig. 2 it can be seen that tetranitro aluminium phthalocyanine is there are two absworption peak, respectively in 356nm and 700nm
Locate, and have the characteristic peak of phthalocyanine dimer at 620nm, the interference that can effectively avoid scattering light is excited at 620nm, obtain
Preferable effect so using 620nm as excitation wavelength, measures solution fluorescence intensity at 700nm wavelength.
(2) detect：The standard solution in step (1) is taken, ascorbic acid solution to be measured is added in into the standard solution, is surveyed
The fluorescence intensity for determining mixed solution is Fx, the linear relationship according to step (1) determines that ascorbic acid contains in solution to be measured
Amount.
Ascorbic acid is detected to tetranitro aluminium phthalocyanine near-infrared fluorescent provided by the invention below in conjunction with specific embodiment
Method be further described.The embodiments described below is exemplary, be only used for explain the present invention, and it is not intended that
Limitation of the present invention.
Embodiment 1：
Influence of the tetranitro aluminium phthalocyanine concentration to fluorescent quenching is present embodiments provided, specific method is as follows：
It is 1.0 × 10 to prepare concentration according to above-described embodiment-3The tetranitro aluminium phthalocyanine solution of g/ml, in 7 5.0ml colorimetrics
The B-R buffer solution 0.25ml of pH=7.0 are respectively added in pipe, respectively pipette 15 μ l, 20 μ l, 25 μ l, 30 μ l, 35 μ l, 40 μ l, 45 μ l
Concentration is 1.0 × 10-3Then the tetranitro aluminium phthalocyanine solution of g/ml is settled to 5.0mL to 1~No. 7 colorimetric cylinder with DMF, i.e., 1~
The concentration of the tetranitro aluminium phthalocyanine solution added in No. 7 colorimetric cylinders is respectively 3 × 10-6G/ml, 4 × 10-6G/ml, 5 × 10-6g/
Ml, 6 × 10-6G/ml, 7 × 10-6G/ml, 8 × 10-6G/ml, 9 × 10-6g/ml.Take 2.00ml above-mentioned containing difference with pipette
The tetranitro aluminium phthalocyanine standard solution of concentration starts luminoscope in luminoscope cuvette, and temperature is set as 30 DEG C, heats up and protects
Warm 3min using 620nm as excitation wavelength, measures solution fluorescence intensity at 700nm wavelength, is denoted as F0；Again to it is above-mentioned containing
It is 1.0 × 10 that concentration is added in the cuvette of various concentration tetranitro aluminium phthalocyanine standard solution-55 μ of ascorbic acid solution of g/ml
L, is 30 DEG C in temperature, and mixed solution is measured during 3min in the fluorescence intensity of 700nm wavelength, is denoted as F.
Measurement result is shown in Fig. 3, as shown in Figure 3：When the concentration of tetranitro aluminium phthalocyanine is 6.0 × 10-6During g/ml, ascorbic acid
To the fluorescent quenching maximum intensity of tetranitro aluminium phthalocyanine, i.e. tetranitro aluminium phthalocyanine optimum concentration is 6.0 × 10-6g/ml。
Embodiment 2：
Influence of the pH value to fluorescent quenching is present embodiments provided, specific method is as follows：
It is 1.0 × 10 to prepare concentration according to above-described embodiment-3The tetranitro aluminium phthalocyanine solution of g/ml, in 9 5.0ml colorimetrics
The B-R buffer solution 0.25ml that pH is 5.5,6.0,6.5,7.0,7.5,8.0,8.5,9.0 and 9.5 are separately added into pipe, are pipetted
30 μ l concentration are 1.0 × 10-3Then the tetranitro aluminium phthalocyanine solution of g/ml is settled to 5.0ml to 1~No. 9 colorimetric cylinder with DMF,
The tetranitro aluminium phthalocyanine standard solution for taking the above-mentioned pH value of 2.00ml different with pipette starts fluorescence in luminoscope cuvette
Instrument, temperature are set as 30 DEG C, heat up and keep the temperature 3min, using 620nm as excitation wavelength, it is glimmering at 700nm wavelength to measure the solution
Luminous intensity is denoted as F0；It is 1.0 × 10 to add in concentration into the different cuvette of above-mentioned pH value again-5The ascorbic acid solution 5 of g/ml
μ l, are 30 DEG C in temperature, and mixed solution is measured during 3min in the fluorescence intensity of 700nm wavelength, is denoted as F.
Measurement result is shown in Table 1, as shown in Table 1：When the pH value of solution is 7.0, ascorbic acid is to tetranitro aluminium phthalocyanine
Fluorescent quenching maximum intensity.
Influence of the table 1.pH values to fluorescent quenching
Embodiment 3：
Influence of the temperature to quenching is present embodiments provided, specific method is as follows：
It is 1.0 × 10 to prepare concentration according to above-described embodiment-3The tetranitro aluminium phthalocyanine solution of g/ml, in 6 5.0ml colorimetrics
The B-R buffer solution 0.25ml that pH is 7.0 are separately added into pipe, pipette 30 μ l concentration as 1.0 × 10-3The tetranitro aluminium phthalein of g/ml
Then cyanines solution is settled to 5.0ml with DMF, the above-mentioned tetranitro aluminium phthalocyanine marks of 2.00ml is taken with pipette to 1~No. 6 colorimetric cylinder
Quasi- solution starts luminoscope in luminoscope cuvette, and temperature is respectively set as 21 DEG C when being put into 6 colorimetric cylinders, 25 DEG C, and 30
DEG C, it 35 DEG C, 40 DEG C, 45 DEG C, heats up and keeps the temperature 3min, using 620nm as excitation wavelength, it is glimmering at 700nm wavelength to measure the solution
Luminous intensity is denoted as F0；It is 1.0 × 10 to add in concentration into above-mentioned cuvette again-5The 5 μ l of ascorbic acid solution of g/ml, exist respectively
Temperature is 21 DEG C, and 25 DEG C, 30 DEG C, 35 DEG C, 40 DEG C, 45 DEG C, fluorescence intensity of the mixed solution in 700nm wavelength is measured during 3min,
It is denoted as F.
Measurement result is shown in Table 2, as shown in Table 2：When temperature is 30 DEG C, ascorbic acid is strong to the quenching of tetranitro aluminium phthalocyanine
Degree is maximum, i.e., optimum measurement temperature is 30 DEG C.
Influence of 2. temperature of table to fluorescent quenching
Embodiment 4：
Influence of the time to fluorescent quenching is present embodiments provided, specific method is as follows：
It is 1.0 × 10 to prepare concentration according to above-described embodiment-3The tetranitro aluminium phthalocyanine solution of g/ml, in 1 5.0ml colorimetric
The B-R buffer solution 0.25ml that pH is 7.0 are added in pipe, pipette 30 μ l concentration as 1.0 × 10-3The tetranitro aluminium phthalocyanine of g/ml is molten
Then liquid is settled to 5.0ml into colorimetric cylinder with DMF, with pipette take the above-mentioned tetranitro aluminium phthalocyanine standard solution of 2.00ml in
In luminoscope cuvette, luminoscope is started, temperature is set as 30 DEG C, heats up and keeps the temperature 3min, using 620nm as excitation wavelength, surveys
Fixed solution fluorescence intensity at 700nm wavelength, is denoted as F0；It is 1.0 × 10 to add in concentration into above-mentioned cuvette again-5G/ml's
When temperature is 30 DEG C, mixed once solution is surveyed in 700nm wavelength from 1~11min every 1min by 5 μ l of ascorbic acid solution
Fluorescence intensity is denoted as F.
Measurement result is shown in Fig. 4, as shown in Figure 4：After ascorbic acid is added in, reaction system started steady at the 3rd minute
Fixed, i.e., optimum time of measuring is 3 minutes.
Embodiment 5：
The present embodiment provides a kind of fluorescence intensities by detecting tetranitro aluminium phthalocyanine, establish fluorescence intensity difference and Vitamin C
The method of linear relationship between acid concentration, it is specific as follows：
Based on Examples 1 to 4, the B-R buffer solution 0.25ml that pH is 7.0 are added in 1 5.0ml colorimetric cylinder, are pipetted
30 μ l concentration are 1.0 × 10-3Then the tetranitro aluminium phthalocyanine solution of g/ml is settled to 5.0ml, with shifting into colorimetric cylinder with DMF
Liquid pipe takes the above-mentioned tetranitro aluminium phthalocyanine standard solution of 2.00ml to start luminoscope, temperature is set as 30 in luminoscope cuvette
DEG C, it heats up and keeps the temperature 3min, using 620nm as excitation wavelength, measure solution fluorescence intensity at 700nm wavelength, be denoted as F0；Again
Be separately added into above-mentioned cuvette 5 μ l, 10 μ l, 15 μ l, 20 μ l, 25 μ l, 30 μ l, 35 μ l, 40 μ l, 45 μ l, 50 μ l, 55 μ l and
60 μ l concentration are 1.0 × 10-5The ascorbic acid solution of g/ml, since the amount of the ascorbic acid of addition is fewer, the volume of solution
Variation is ignored, i.e. the concentration of ascorbic acid solution (AA) is followed successively by 2.50 × 10-8G/ml, 5.00 × 10-8G/ml, 7.50
×10-8G/ml, 1.00 × 10-7G/ml, 1.25 × 10-7G/ml, 1.50 × 10-7G/ml, 1.75 × 10-7G/ml, 2.00 × 10- 7G/ml, 2.25 × 10-7G/ml, 2.50 × 10-7G/ml, 2.75 × 10-7G/ml and 3.00 × 10-7G/ml is 30 DEG C in temperature
When, mixed solution is measured during 3min in the fluorescence intensity of 700nm wavelength, is denoted as F.
Measurement result is shown in Fig. 5, with CAAFor abscissa, using △ F as ordinate, △ F are F0- F, draws linear graph, and wherein B is
Obtained linear graph, Data 1B are the standard curve obtained after being fitted.As shown in Figure 5：When ascorbic acid concentration for 2.5 ×
10-8~3.0 × 10-7During g/ml, in good linear relationship, equation of linear regression is the concentration of △ F and ascorbic acid：△ F=
42.095×107CAA+ 36.855, correlation coefficient r=0.9996, wherein CAAUnit be 1.0 × 10-7g/ml；Detection is limited to
7.6×10-9g/ml。
Embodiment 6：
Ascorbic acid content and mark-on in sample are detected the present embodiment provides a kind of tetranitro aluminium phthalocyanine near-infrared fluorescent to return
The method of yield, it is specific as follows：
(1) 3,0.1g vitamin Cs tablet is taken, is ground, mixing weighs three parts, and is dissolved in 20ml deionized waters, obtains
Vitamin C dispersion liquid after 10000r/min centrifuges 20min, through 0.22um membrane filtrations, collects supernatant, is transferred to 250ml capacity
Constant volume in bottle, takes 1.0ml to be diluted to scale with distilled water in 100ml volumetric flasks and shakes up, and it is spare that testing sample solution is made.
Above-mentioned vitamin C tablet is the Wei Fujia board vitamin Cs selected from Zhejiang Rui Xin medicine companies limited company.
(2) pure three parts of ascorbic acid analysis is weighed, and concentration is made into as 1.0 × 10 with redistilled water-5G/ml's is anti-bad
Hematic acid analyzes pure solution for standby.
(3) 3 10ml colorimetric cylinders are taken, it is 1.0 × 10 to be separately added into the B-R buffer solutions 0.25ml of pH=7.0 and concentration- 3The 30 μ l of tetranitro aluminium phthalocyanine solution of g/ml, 5.0ml is settled to DMF；It pipettes respectively molten in above-mentioned 3 colorimetric cylinders of 2.0ml
For liquid in cuvette, temperature is set as 30 DEG C, heats up and keeps the temperature 3min, using 620nm as excitation wavelength, measures the solution in 700nm
Fluorescence intensity at wavelength, is denoted as F0。
(4) testing sample solution prepared in 10 μ l steps (1), temperature are separately added into 3 cuvettes in step (3)
Degree is set as 30 DEG C, heats up and keeps the temperature 3min, using 620nm as excitation wavelength, it is strong to measure solution fluorescence at 700nm wavelength
Degree, is denoted as F1。
(5) it is 1.0 × 10 that the concentration prepared in 10 μ l steps (2) is separately added into 3 cuvettes in step (4)- 5The ascorbic acid of g/ml analyzes pure solution, and temperature is set as 30 DEG C, heats up and keep the temperature 3min, using 620nm as excitation wavelength, surveys
Fixed solution fluorescence intensity at 700nm wavelength, is denoted as F2。
Pass through the linear relationship determined in embodiment 5, it can be deduced that the content of ascorbic acid in sample.Measurement result is shown in Table
3, as shown in Table 3：Ascorbic acid mass fraction in institute's sample VITAMIN C TABLET is 83.60%, the rate of recovery for 97%~
102%.
Table 3 measures ascorbic acid content and recovery of standard addition in sample
The technical program is fluorescence probe detection ascorbic acid content with tetranitro aluminium phthalocyanine, as seen from Figure 1：Four nitre
The emission spectrum of base aluminum phthalocyanine is located near infrared region, can be reduced by the use of tetranitro aluminum phthalocyanine as fluorescence probe in detection process
Interference and raising sensitivity.The technical program directly establishes the fluorescence quenching of tetranitro aluminium phthalocyanine using ascorbic acid glimmering
Linear relationship between light intensity difference and ascorbic acid concentrations, i.e. △ F=42.095 × 107CAA+ 36.855, and it is linear to pass through this
The method that relation determines ascorbic acid content in sample to be tested, it is easy to operate；The concentration of ascorbic acid is 2.5 × 10-8~3.0 ×
10-7G/ml, detection are limited to 7.6 × 10-9G/ml has wider detection range and relatively low detection limit, improves in detection process
Sensitivity；And the 3min after ascorbic acid is added in can be detected, detection time is short；From Examples 1 to 6, examining
The optium concentration of tetranitro aluminium phthalocyanine is 6.0 × 10 during survey-6G/ml, optimal pH 7.0, measurement fluorescence intensity are most preferably warm
It is 3min to spend for 30 DEG C and optimal measuring time.
Finally, method of the invention is only preferable embodiment, is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modifications, equivalent replacements and improvements are made should be included in the protection of the present invention
Within the scope of.
Claims (8)
- A kind of 1. method of tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid, which is characterized in that comprise the following steps：S1. linear relationship is established：Prepare the identical tetranitro aluminium phthalocyanine standard solution of more parts of concentration, the fluorescence of bioassay standard solution Intensity is F0, into each part standard solution, the ascorbic acid solution of addition various concentration, the fluorescence for measuring each part mixed solution are strong It spends for Fi, establish F0-FiAnd CiBetween linear relationship, wherein CiFor the concentration of ascorbic acid solution；S2. detect：The standard solution in step S1 is taken, ascorbic acid solution to be measured is added in into the standard solution, is measured mixed The fluorescence intensity for closing solution is Fx, the linear relationship according to step S1 determines ascorbic acid content in solution to be measured.
- 2. the method for tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid according to claim 1, which is characterized in that The pH value of the step S1 and step S2 Plays solution is 5.5~9.5, and tetranitro aluminium phthalocyanine concentration is 3 × 10-6~9 × 10-6g/ml。
- 3. the method for tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid according to claim 2, which is characterized in that The pH value of the step S1 and step S2 Plays solution is 7.0, and tetranitro aluminium phthalocyanine concentration is 6 × 10-6g/ml。
- 4. the method for tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid according to claim 1, which is characterized in that Measuring temperature is 21~45 DEG C when fluorescence intensity is measured in the step S1, and minute is 1~11min.
- 5. the method for tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid according to claim 4, which is characterized in that Measuring temperature is 30 DEG C when fluorescence intensity is measured in the step S1, minute 3min.
- 6. the method for tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid according to claim 1, which is characterized in that In the step S1 and S2, fluoroscopic examination excitation wavelength is 620nm, measures solution to be measured fluorescence intensity at 700nm wavelength.
- 7. the method for tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid according to claim 1, which is characterized in that The F0-FiAnd CiBetween linear relationship be F0-Fi=42.095 × 107Ci+ 36.855, wherein CiFor 2.5 × 10-8~3.0 ×10-7g/ml。
- 8. the method for tetranitro aluminium phthalocyanine near-infrared fluorescent detection ascorbic acid according to claim 1, which is characterized in that Tetranitro aluminium phthalocyanine is limited to 7.6 × 10 to the detection of ascorbic acid concentrations in solution to be measured-9g/ml。
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| CN114113018A (en) * | 2021-11-30 | 2022-03-01 | 厦门大学 | Fluorescence detection method for determining zinc ions by taking tetranitrophthalocyanine as reagent |
| CN114113018B (en) * | 2021-11-30 | 2023-10-24 | 厦门大学 | Fluorescent detection method for measuring zinc ions by taking tetranitrophthalocyanine as reagent |
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