CN108051277B - Reaction device capable of improving cell staining efficiency - Google Patents
Reaction device capable of improving cell staining efficiency Download PDFInfo
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- CN108051277B CN108051277B CN201711221644.3A CN201711221644A CN108051277B CN 108051277 B CN108051277 B CN 108051277B CN 201711221644 A CN201711221644 A CN 201711221644A CN 108051277 B CN108051277 B CN 108051277B
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- 238000006243 chemical reaction Methods 0.000 title claims abstract description 19
- 238000010186 staining Methods 0.000 title claims abstract description 12
- 238000010438 heat treatment Methods 0.000 claims description 7
- 230000000087 stabilizing effect Effects 0.000 claims description 6
- 230000008878 coupling Effects 0.000 claims 1
- 238000010168 coupling process Methods 0.000 claims 1
- 238000005859 coupling reaction Methods 0.000 claims 1
- 238000004043 dyeing Methods 0.000 abstract description 33
- 238000000034 method Methods 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 14
- 238000002474 experimental method Methods 0.000 abstract description 9
- 239000003086 colorant Substances 0.000 abstract description 5
- 230000008595 infiltration Effects 0.000 abstract description 4
- 238000001764 infiltration Methods 0.000 abstract description 4
- 210000004027 cell Anatomy 0.000 description 48
- 238000012360 testing method Methods 0.000 description 17
- 239000000975 dye Substances 0.000 description 7
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 108010077544 Chromatin Proteins 0.000 description 3
- 241000270295 Serpentes Species 0.000 description 3
- 210000003483 chromatin Anatomy 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 210000000633 nuclear envelope Anatomy 0.000 description 3
- 210000004940 nucleus Anatomy 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 210000002472 endoplasmic reticulum Anatomy 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000003793 centrosome Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 210000002288 golgi apparatus Anatomy 0.000 description 1
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- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
Abstract
The invention discloses a reaction device capable of improving cell staining efficiency, which comprises a base, wherein supporting legs are fixedly connected to the top of the base, a fixing plate is fixedly connected to the top ends of the supporting legs, supporting blocks are fixedly connected to the back of the fixing plate, a vibration block is rotatably connected to the front of each supporting block, a fixing device is fixedly connected to the front of the vibration block, the fixing device comprises a fixing block and a connecting block, a rotating rod is rotatably connected to the inside of the fixing block, a moving block is in threaded connection with the surface of the rotating rod, and a limiting rod is slidably connected to the surface of the moving block. This can improve reaction unit of cell dyeing efficiency has changed the in-process of cell dyeing because the inside of coloring agent infiltration cell is too slow, leads to the problem that the cell dyeing time is long, has improved the efficiency of cell dyeing and the effect of cell dyeing greatly, has guaranteed the progress and the experimental result of experiment.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a reaction device capable of improving cell staining efficiency.
Background
The biotechnology means that people adopt advanced scientific and technological means on the basis of modern life science and by combining with scientific principles of other basic sciences, according to the design of the prior art, the biological raw material is reformed or processed to produce the required product for human or achieve a certain purpose, the biotechnology is that the human uses microorganisms, animals and plants to process the material raw material, the technology for providing products to serve the society mainly comprises fermentation technology and modern biotechnology, therefore, biotechnology is an emerging and comprehensive subject, and modern biotechnology integrates multiple disciplines technologies such as genetic engineering, molecular biology, biochemistry, genetics, cell biology, embryology, immunology, organic chemistry, inorganic chemistry, physical chemistry, physics, informatics, computer science and the like, and can be used for researching the rules of life activities, providing products for social services and the like.
Like other systems, cells are also bordered, have several components that cooperate in division, and have information centers that regulate the metabolism and inheritance of cells. The structure of the cell is complex and exquisite, and various structural components are coordinated, so that the life activities can be self-regulated and highly orderly carried out in a changing environment, and the organelles of the biological cell comprise: endoplasmic reticulum, mitochondria, golgi apparatus, ribosomes, lysosomes, centrosomes, most cells have only one nucleus, and some cells contain two or more nuclei, such as myocytes, hepatocytes, and the like. The nucleus can be divided into four parts, namely nuclear membrane, chromatin, nuclear fluid and nucleolus. The nuclear membrane is communicated with endoplasmic reticulum, the chromatin is located between the nuclear membrane and the nucleolus, and the chromatin is mainly composed of protein and DNA.
In the biomedical experiment or some physical and chemical experiments, reasonable coloring agents are usually selected for better observing the experimental effect, and the expected experimental phenomenon or result can be well observed after the proper coloring agents are selected. The dyeing agent can be divided into natural dyeing agent and artificial synthesis dyeing agent according to the source, different dyeing agents are selected in different experiments, the same dyeing agent can be prepared by different formulas, but in the cell dyeing process, the cell dyeing time is long due to the fact that the dyeing agent penetrates into the cell too slowly, the cell dyeing efficiency is slow, the cell dyeing effect is reduced, and the experiment progress and the experiment result are influenced.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a reaction device capable of improving the cell staining efficiency, and solves the problem that the cell staining time is long due to the fact that a staining agent permeates into the inside of a cell too slowly in the cell staining process.
(II) technical scheme
In order to achieve the purpose, the invention is realized by the following technical scheme: a reaction device capable of improving cell staining efficiency comprises a base, wherein supporting legs are fixedly connected to the top of the base, a fixing plate is fixedly connected to the top ends of the supporting legs, supporting blocks are fixedly connected to the back face of the fixing plate, a vibration block is rotatably connected to the front face of each supporting block, a fixing device is fixedly connected to the front face of each vibration block, each fixing device comprises a fixing block and a connecting block, a rotating rod is rotatably connected to the inside of each fixing block, a movable block is in threaded connection with the surface of each rotating rod, a limiting rod is slidably connected to the surface of each movable block, two ends of each limiting rod are fixedly connected to the surface of each fixing block, a sliding groove is formed in the front face of each connecting block, a sliding block is slidably connected to the inner wall of each sliding groove, a connecting rod is, the front of slider fixedly connected with fixture block to the equal fixedly connected with rubber pad in the relative one side of two fixture blocks, the equal fixedly connected with turning block in both ends of dwang.
Preferably, the surface of the vibration block is fixedly connected with a heating wire, and the bottom of the base is fixedly connected with a fixing leg.
Preferably, the top of the base is fixedly connected with a motor, an output shaft of the motor is fixedly connected with a turntable through a coupler, and the surface of the turntable is provided with a moving groove.
Preferably, the top of the base is fixedly connected with a stabilizing block, the surface of the stabilizing block is fixedly connected with a shaft block, and the surface of the shaft block is slidably connected with a movable rod.
Preferably, a fixing groove is formed on the surface of the moving rod, and the inner wall of the fixing groove is slidably connected with the surface of the shaft block.
Preferably, a contact block is fixedly connected to the surface of the moving rod, and a contact rod is fixedly connected to the surface of the contact block, and the surface of the contact rod is slidably connected to the inner wall of the moving groove.
Preferably, a driving block is fixedly connected to the surface of the moving rod, a shaft rod is slidably connected to the inside of the driving block, and one end of the shaft rod is fixedly connected to the surface of the vibrating block.
(III) advantageous effects
The invention provides a reaction device capable of improving cell staining efficiency. The method has the following beneficial effects:
(1) the reaction device capable of improving the cell dyeing efficiency comprises a rotating block, a rotating rod, a connecting block, a sliding groove, a sliding block, a connecting block, a vibrating block and a motor, wherein a test tube which is sealed and filled with a reagent is placed between two clamping blocks, the rotating block is rotated to drive the rotating rod to rotate, opposite threads are arranged on the surface of the rotating rod to drive two moving blocks to move relatively, the test tube is clamped and fixed under the action of the connecting block, the motor drives the rotating disk to rotate, the surface of the rotating disk is provided with a moving groove, the surface of a contact rod is in sliding connection with the inner wall of the moving groove, the moving rod is made to do reciprocating motion, the moving rod drives a driving block and a shaft rod to make the vibrating block rotate in a reciprocating manner to drive the test tube to vibrate repeatedly, the dyeing of cells is accelerated, the problem that, the experimental progress and the experimental result are ensured.
(2) This can improve reaction unit of cell dyeing efficiency heats the test tube through heating wire and improves the temperature, makes the boa snake effect of cell better, has improved dyeing efficiency, and rethread supporting leg, fixed plate and supporting shoe make the structure of device more stable, have changed the single structure of the device on the market, greatly increased its functionality, whole operation process is simple and convenient.
Drawings
FIG. 1 is a drawing of the present invention;
FIG. 2 is a back view of the structure of the present invention;
FIG. 3 is a schematic view of a fixing device according to the present invention;
fig. 4 is a partial enlarged view of the invention taken at a in fig. 3.
In the figure, 1 base, 2 supporting legs, 3 fixing plates, 4 supporting blocks, 5 vibration blocks, 6 fixing devices, 61 fixing blocks, 62 connecting blocks, 63 rotating rods, 64 moving blocks, 65 limiting rods, 66 sliding grooves, 67 sliding blocks, 68 connecting rods, 69 clamping blocks, 610 rubber pads, 611 rotating blocks, 7 heating wires, 8 fixing legs, 9 motors, 10 rotating discs, 11 moving grooves, 12 shaft rods, 13 stabilizing blocks, 14 shaft blocks, 15 moving rods, 16 fixing grooves, 17 contact blocks, 18 contact rods and 19 driving blocks.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Referring to fig. 1-4, an embodiment of the present invention provides a technical solution: a reaction device capable of improving cell staining efficiency comprises a base 1, wherein supporting legs 2 are fixedly connected to the top of the base 1, fixed plates 3 are fixedly connected to the top ends of the supporting legs 2, supporting blocks 4 are fixedly connected to the back of the fixed plates 3, vibrating blocks 5 are rotatably connected to the front of the supporting blocks 4, fixing devices 6 are fixedly connected to the front of the vibrating blocks 5, each fixing device 6 comprises a fixed block 61 and a connecting block 62, a rotating rod 63 is rotatably connected to the inside of the fixed block 61, a moving block 64 is in threaded connection with the surface of the rotating rod 63, a limiting rod 65 is in sliding connection with the surface of the moving block 64, two ends of the limiting rod 65 are fixedly connected with the surface of the fixed block 61, a sliding groove 66 is formed in the front of the connecting block 62, a sliding block 67 is in sliding connection with the inner wall of the sliding groove 66, a connecting rod 68, the front of the slide block 67 is fixedly connected with a fixture block 69, a test tube which is sealed and is filled with a reagent is placed between the two fixture blocks 69, the rotating block 611 is rotated to drive the rotating rod 63 to rotate, the surface of the rotating rod 63 is provided with opposite threads to drive the two moving blocks 64 to move relatively, the test tube is clamped and fixed under the action of the connecting block 62, the sliding groove 66, the slide block 67 and the connecting block 68, a rubber pad 610 is fixedly connected to one side of the two fixture blocks 69 which is opposite to each other to prevent the test tube from being broken, the rotating block 611 is fixedly connected to both ends of the rotating rod (3), the heating wire 7 is fixedly connected to the surface of the vibrating block 5, the test tube is heated by the heating wire 7 to improve the temperature, the snake effect of cells is better, the dyeing efficiency is improved, and the structure of the device is more stable through the supporting legs, the single, the whole operation process is simple and convenient, the bottom of the base 1 is fixedly connected with a fixed leg 8, the top of the base 1 is fixedly connected with a motor 9, the output shaft of the motor 9 is fixedly connected with a turntable 10 through a coupler, the surface of the turntable 10 is provided with a moving groove 11, the top of the base 1 is fixedly connected with a stabilizing block 13, the surface of the stabilizing block 13 is fixedly connected with a shaft block 14, the surface of the shaft block 14 is slidably connected with a moving rod 15, the surface of the moving rod 15 is provided with a fixed groove 16, the inner wall of the fixed groove 16 is slidably connected with the surface of the shaft block 14, the motor 9 drives the turntable 10 to rotate, the surface of the turntable 10 is provided with a moving groove 11, the surface of a contact rod 18 is slidably connected with the inner wall of the moving groove 11, the moving rod 15 is made to do reciprocating motion, the moving rod 15 drives a driving block 19 and a shaft lever, accelerate the dyeing of cell, the in-process that has changed the cell dyeing is because the inside of coloring agent infiltration cell is too slow, lead to the long problem of cell dyeing time, the efficiency of cell dyeing and the effect of cell dyeing have been improved, the progress and the experimental result of experiment have been guaranteed, the fixed surface of carriage release lever 15 is connected with contact block 17, and the fixed surface of contact block 17 is connected with contact lever 18, the surface of contact lever 18 and the inner wall sliding connection of shifting chute 11, the fixed surface of carriage release lever 15 is connected with drive block 19, and the inside sliding connection of drive block 19 has axostylus axostyle 12, the one end of axostylus axostyle 12 is connected with the fixed surface of vibrations piece 5.
When the device is used, a user puts a test tube which is sealed and filled with a reagent between the two clamping blocks 69, the rotating block 611 is rotated to drive the rotating rod 63 to rotate, opposite threads are arranged on the surface of the rotating rod 63 to drive the two moving blocks 64 to move relatively, the test tube is clamped and fixed under the action of the connecting block 62, the sliding groove 66, the sliding block 67 and the connecting block 68, a rubber pad 610 is fixedly connected to one side, opposite to the two clamping blocks 69, to prevent the test tube from being broken, the test tube is heated by the heating wire 7 to improve the temperature, the bobble snake effect of cells is better, the dyeing efficiency is improved, and the structure of the device is more stable through the supporting legs, the fixing plates and the supporting blocks, the single structure of the device in the market is changed, the functionality is greatly increased, the whole operation process is simple and convenient, the rotating disc 10 is, the surface of contact lever 18 and the inner wall sliding connection of moving bath 11, make the carriage release lever 15 be reciprocating motion, carriage release lever 15 drives drive block 19 and axostylus axostyle 12, make 5 reciprocating rotation of vibrations piece, drive the vibration that the test tube relapse, accelerate the dyeing of cell, the in-process that has changed cell dyeing is too slow because the inside of coloring agent infiltration cell, lead to the problem that cell dyeing time is long, the efficiency of cell dyeing and the effect of cell dyeing have been improved, the progress and the experimental result of experiment have been guaranteed, just so the dyeing of cell has been accomplished
To sum up the above
This can improve reaction unit of cell dyeing efficiency, through putting the sealed test tube that is equipped with the reagent between two fixture blocks 69, it drives dwang 63 to rotate turning block 611, the surface of dwang 63 is equipped with opposite screw thread, drive two movable blocks 64 relative motion, make the test tube chucking fixed under the effect of connecting block 62, spout 66, slider 67 and connecting block 68, heat the test tube through heater strip 7 and improve the temperature, make the bobble snake effect of cell better, improve dyeing efficiency, rethread supporting leg, fixed plate and supporting shoe, make the structure of device more stable, the single structure of the device in the market has been changed, its functionality has been greatly increased, the whole operation process is simple and convenient, it rotates to drive carousel 10 through motor 9 again, shifting chute 11 has been seted up on the surface of carousel 10, the surface of contact bar 18 and the inner wall sliding connection of shifting chute 11, make the carriage release lever 15 be reciprocating motion, carriage release lever 15 drives drive block 19 and axostylus axostyle 12, makes 5 reciprocating rotation of vibrations piece, drives the shock that the test tube is repeated for the dyeing of cell has changed the in-process of cell dyeing because the inside of dyeing agent infiltration cell is too slow, leads to the problem that cell dyeing time is long, has improved the efficiency of cell dyeing and the effect of cell dyeing, has guaranteed the progress and the experimental result of experiment.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation. The use of the phrase "comprising one of the elements does not exclude the presence of other like elements in the process, method, article, or apparatus that comprises the element.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (7)
1. The utility model provides an improve reaction unit of cell staining efficiency, includes base (1), its characterized in that: the base is characterized in that the top of the base (1) is fixedly connected with supporting legs (2), the top ends of the supporting legs (2) are fixedly connected with a fixing plate (3), the back face of the fixing plate (3) is fixedly connected with a supporting block (4), the front face of the supporting block (4) is rotatably connected with a vibration block (5), the front face of the vibration block (5) is fixedly connected with a fixing device (6), the fixing device (6) comprises a fixing block (61) and a connecting block (62), the inside of the fixing block (61) is rotatably connected with a rotating rod (63), the surface of the rotating rod (63) is in threaded connection with a moving block (64), the surface of the moving block (64) is in sliding connection with a limiting rod (65), two ends of the limiting rod (65) are fixedly connected with the surface of the fixing block (61), the front face of the connecting block (62) is provided with a sliding groove, the front face of the sliding block (67) is rotatably connected with a connecting rod (68), one end of the connecting rod (68) is rotatably connected with the front face of the moving block (64), the front face of the sliding block (67) is fixedly connected with a clamping block (69), one side, opposite to the two clamping blocks (69), of the connecting rod is fixedly connected with a rubber pad (610), and two ends of the rotating rod (63) are fixedly connected with rotating blocks (611).
2. The reaction device of claim 1, wherein: the surface of the vibration block (5) is fixedly connected with a heating wire (7), and the bottom of the base (1) is fixedly connected with a fixing leg (8).
3. The reaction device of claim 1, wherein: the top fixedly connected with motor (9) of base (1) to the output shaft of motor (9) passes through shaft coupling fixedly connected with carousel (10), shifting chute (11) have been seted up on the surface of carousel (10).
4. The reaction device of claim 1, wherein: the top fixedly connected with of base (1) stabilizes piece (13) to the fixed surface of stabilizing piece (13) is connected with axle piece (14), the surperficial sliding connection of axle piece (14) has carriage release lever (15).
5. The reaction device of claim 4, wherein: the surface of the moving rod (15) is provided with a fixing groove (16), and the inner wall of the fixing groove (16) is connected with the surface of the shaft block (14) in a sliding manner.
6. The reaction device of claim 4, wherein: the surface of the moving rod (15) is fixedly connected with a contact block (17), the surface of the contact block (17) is fixedly connected with a contact rod (18), and the surface of the contact rod (18) is in sliding connection with the inner wall of the moving groove (11).
7. The reaction device of claim 4, wherein: the surface of the moving rod (15) is fixedly connected with a driving block (19), the inside of the driving block (19) is connected with a shaft rod (12) in a sliding mode, and one end of the shaft rod (12) is fixedly connected with the surface of the vibration block (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711221644.3A CN108051277B (en) | 2017-11-25 | 2017-11-25 | Reaction device capable of improving cell staining efficiency |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711221644.3A CN108051277B (en) | 2017-11-25 | 2017-11-25 | Reaction device capable of improving cell staining efficiency |
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| CN108051277A CN108051277A (en) | 2018-05-18 |
| CN108051277B true CN108051277B (en) | 2020-03-27 |
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| CN201711221644.3A Expired - Fee Related CN108051277B (en) | 2017-11-25 | 2017-11-25 | Reaction device capable of improving cell staining efficiency |
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2017
- 2017-11-25 CN CN201711221644.3A patent/CN108051277B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
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| US5610069A (en) * | 1992-03-27 | 1997-03-11 | Abbott Laboratories | Apparatus and method for washing clinical apparatus |
| KR20070046822A (en) * | 2004-06-23 | 2007-05-03 | 베르뗑 떼끄놀로지 에스.아. | Device for guiding a shaft in an oscillating movement |
| WO2010038852A1 (en) * | 2008-10-03 | 2010-04-08 | アークレイ株式会社 | Sample stirring device, liquid chromatography device using same, and sample container stand |
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| CN108051277A (en) | 2018-05-18 |
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