CN108047318A - A kind of active components of glossy ganoderma peptidoglycan, peptidoglycan reference substance and peptidoglycan isolation and purification method - Google Patents
A kind of active components of glossy ganoderma peptidoglycan, peptidoglycan reference substance and peptidoglycan isolation and purification method Download PDFInfo
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Abstract
The invention discloses a kind of active components of glossy ganoderma peptidoglycan, peptidoglycan reference substance and peptidoglycan isolation and purification methods, ganoderma lucidum is crushed, water soak extraction obtains ganoderma lucidum aqueous extract, pass through UF membrane of the purified treatment through molecular level, the high molecular weight permeate retained using 10KD dialysis membranes, it is separated through ethanol gradient, 10 pillar layer separation of Bio GL, P, purifying obtains active material peptidoglycan reference substance, and Components identification is carried out under the conditions of high efficiency chromatography and taste differentiates and assay.The present invention using molecular weight membrane separating method low molecule part bitter taste separate and traditional ethanol extraction comparison, risk is small, safe, has no toxic side effect, and is easy to the method for industrialized production.
Description
Technical field
The present invention relates to a kind of active components of glossy ganoderma isolation and purification method, particularly a kind of active components of glossy ganoderma peptidoglycan,
Peptidoglycan reference substance and peptidoglycan isolation and purification method.
Background technology
Domestic and foreign scholars' research shows：The chemical active ingredient of composition ganoderma lucidum has polysaccharide（Peptide）, triterpene, alkaloid, sterol
Etc. there is tens kinds of ingredients, for high score sub-portion peptidoglycan and low molecule triterpenes group isolation and purification, such as He person of outstanding talent, Chen Yan,
The experimental study of the multiple index evaluations such as Du Meng micro emulsion extraction ganoderma lucidum active principle, Chinese patent drug, 2013,35（3）：479~482.Mesh
Before, industrialized producing technology mainly uses water boiling and extraction polysaccharide and polysaccharide peptide composition.Such as：Li Yan, Zhao Haiyan, Lv Jianning spirits
Sesame polysaccharide extracting process research Chinese patent drugs, 2006,28（7）：1050-1054.Such as Lin Shuqian, Wang Saizhen, Lin Zhibin waits grass to plant
Ganoderma polysaccharide peptide extraction conditions research edible mushroom journals, 2003.10（1）：33~36
From 1979, ganoderma lucidum paper 1087 was recorded in American National medical book shop Pubmed websites so far, wherein in November, 2008
Ganoderma lucidum paper 520, same interim National IP Network are just recorded in December, 2012（CNKI）Medicine and agricultural science and technology record ganoderma lucidum and grind
Study carefully paper 2020.Large, the doctor's thesis being the theme with ganoderma lucidum is recorded more than 300, more than numerous studies paper and state
Inside and outside patent document has carried out substantial amounts of research and development to ganoderma lucidum purification, but still can not solve the complicated ingredient of existing ganoderma lucidum
In the bitterness problem that contains.
The content of the invention
It is an object of the invention to overcome the deficiencies in the prior art part, and provide bitter taste low molecule in a kind of removing ganoderma lucidum
Complicated ingredient and its impurity.
The present invention, which also resides in, provides a kind of active components of glossy ganoderma peptidoglycan reference substance.
The present invention, which also resides in, provides a kind of active components of glossy ganoderma peptidoglycan separation and purification process.
A kind of active components of glossy ganoderma peptidoglycan, the ganoderma active polysaccharide peptide peak molecular weight MPFor 1.0 × 104Da with
On ganoderma polysaccharide peptide high-molecular compound.
The ganoderma polysaccharide peptide high-molecular compound of gained, by two or more different monose and 17 kinds of Amino acid profiles.
Log glossy ganoderma peptidoglycan is made of glucose and mannose；Ganoderma polysaccharide peptide is made of glucose and mannose,
Molar ratio is glucose: mannose=31.85: 1.00.
Ganoderma polysaccharide peptide high-molecular compound is classified alcohol precipitation by a kind of active components of glossy ganoderma peptidoglycan reference substance through GL-PP,
Bio-Gl, P-10 pillar layer separation obtain ganoderma active polysaccharide peptide GL-PPSQ sterlings, and purity is more than 97%, and a point position molecular weight is
6.25×104 Da。
A kind of ganoderma active polysaccharide peptide isolation and purification method, by purified lucidum extracting liquid using 10KD UF membranes or thoroughly
Analyse bag, retention peak molecular weight MP1.0×104More than Da, gained are ganoderma polysaccharide peptide high-molecular compound.
By purified lucidum extracting liquid using 10KD UF membranes column or bag filter, retention 10KD molecular weight MP1.0 × 104
Above ingredient is ganoderma polysaccharide peptide high-molecular compound, and due to not containing low molecular compound, and low molecular compound is spirit
The source of sesame bitter taste, therefore the ganoderma polysaccharide peptide high-molecular compound that the isolation and purification method of the present invention is retained does not contain hardship
Taste, change in the prior art ganoderma active composition because of the situation for causing actually to find no sale containing bitter taste.
In summary, present invention advantage following compared with prior art：
Low molecule bitter taste is separated using molecular weight UF membrane by the present invention and traditional ethanol extracts, and risk is small, safe, nothing
Toxic side effect is easy to the method for industrialized production.
The present invention solves Ganodenna Lucidum P.E complicated ingredient in the prior art and is not sufficiently separated and causes Ganodenna Lucidum P.E
It can not be acceptable to the market containing bitter taste, separate molecular weight new technology by the modern times, functional component is retained from molecular level, remove
The method of non-active ingredient is that current art technology is leading.The present invention compensates for existing ganoderma lucidum complexity Multiple components and mouthfeel
It is difficult to separated technical barrier.
The water-soluble active components of glossy ganoderma of molecular level of the present invention is clear and definite, quality controllable, can be applied to product development.It is produced
Product have ganoderma polysaccharide peptide pulvis, oral liquid etc..
Description of the drawings
Fig. 1 of Fig. 1 present invention is the HPLC figures of ganoderma polysaccharide peptide in log glossy ganoderma extracting solution.
The HPLC figures of ganoderma polysaccharide peptide in Fig. 2 JUNCAO Ganoderma lucidum extracting solutions.
Fig. 3 JUNCAO Ganoderma lucidum extract molecular weight determinations（Molecular weight is more than more than 10KD）.
Fig. 4 log glossy ganoderma extract molecular weight determinations（Molecular weight is more than more than 10KD）.
Fig. 5 JUNCAO Ganoderma lucidums fine powder dialysis retention high score sub-portion.
Fig. 6 ganoderma polysaccharide peptide GL-PPSQ amino acid collection of illustrative plates.
Fig. 7 ganoderma polysaccharide peptides GLPPSQ1H-NMR collection of illustrative plates.
Fig. 8 ganoderma polysaccharide peptides GLPPSQ13C-NMR collection of illustrative plates.
Fig. 9 heteronuclear Multiple-quantum Correlated Spectroscopy CHMQC collection of illustrative plates.
Figure 10 HSQC-TOCSY（Hydrocarbon correlation figure-total correlation figure）：
The COSY collection of illustrative plates of Figure 11 GLPPSQ（Same core two-dimensional spectrum).
The HMBC collection of illustrative plates of Figure 12 GLPPSQ.
The NOESY collection of illustrative plates of Figure 13 GLPPSQ（The related figure of space hydrogen hydrogen）.
Figure 14 log glossy ganoderma peptidoglycans monose forms（Molecular weight ranges ＞ 10KD）Figure.
Figure 15 JUNCAO Ganoderma lucidum peptidoglycans monose forms（Molecular weight ranges ＞ 10KD）Figure.
Figure 16 ganoderma polysaccharide peptide content detection canonical plottings.
Figure 17 is that the step of oral liquid is made in ganoderma active polysaccharide peptide product is schemed.
Specific embodiment
The present invention is described in more detail with reference to embodiment.
Embodiment 1
A kind of Ganodenna Lucidum P.E stoste TuPu method, under the conditions of specific collection of illustrative plates, Ganodenna Lucidum P.E is more with a variety of macromolecules
Glycopeptide, retention time is 11.875 ' -15.297 ', peak molecular weight MP1.5×104 -4.8×105 Da (see Fig. 1 and Fig. 2).
A kind of ganoderma active polysaccharide peptide, the ganoderma polysaccharide peptide, peak molecular weight is in MP1.0×104The spirit of more than Da
Sesame peptidoglycan high-molecular compound.
Log glossy ganoderma extracting solution is under specific chromatographic condition, and retention time 8.093 is divided, Mp2.8 × 104 Da, Mn1.3 ×
104 Da, Mw2.0 × 104Da, area 94.91%（See Fig. 3）For log glossy ganoderma macromolecule polysaccharide peptide.
Peptidoglycan retention time 8.731 is divided in JUNCAO Ganoderma lucidum extract, Mp1.0 × 104 Da Mn1.1×104Da, Mw1.5
×104Da, area 63.98%（See Fig. 4）For macromolecule JUNCAO Ganoderma lucidum peptidoglycan.
JUNCAO Ganoderma lucidum extract（Fine powder）, collected by 10KD under equal chromatographic condition, appearance retention time 8.693
Point, Mp1.1 × 104Da, Mn1.1 × 104Da, Mw1.6 × 104Da, area 56.50%（See Fig. 5）, it is also the same to obtain spirit
Sesame peptidoglycan.
The chemical constitution of ganoderma active polysaccharide peptide GL-PPSQ：Its structure feature, main chain is by glucose group into -4) Glcp
(1-, -3) Glcp(1- and -6) Glcp(main chain that 1- is formed, -6) Glcp(the 4 Wei Shangyou branches of 1-.In structure on peptide chain
Serine or the monose that is connected of hydroxyl of threonine be O-glycosides key connection.GLPPSQ is a kind of active polysaccharide peptide, and yield is
The 0.53% of ganoderma lucidum fruitbody.Its purity more than 97%, containing polysaccharide 88.18% and 17 kind of Amino acid profile, total amino acid content is
4.03%（See Fig. 6）.
Active components of glossy ganoderma peptidoglycan GLPPSQ Structure Deduction foundations, pass through1H（Fig. 7）、13C（Fig. 8）With a variety of two-dimensional nucleus
Magnetic COSY, HSQC, HSQC-TOCSY, NOESY and HMBC（Fig. 9-13）Spectrogram is measured, is methylated point in combination with GC-MS
The glycosyl of analysis and GLPPSQ form.
According to gas phase-mass spectrographic result：To speculate, monose puts in order in polysaccharide, main chain, branch and the tie point of polysaccharide.
Experiment condition：GC-MS systems（Thermo Finnigans company, model：Trace2000/MS）, chromatographic column：DB-5MS（30m ×
0.25 mm, 0.25 μm, 0.2 mm film thickness）（It see the table below 1）.
The analysis result of glucose residue in the methylation analysis of 1 polysaccharide GLPPSQ of table
| Retention time（min） | Methylate fragment | The type of glycosidic bond | Molar ratio |
| 12.794 | 2,3,4,6-Me4-Glc p | Glc p-(1→ | 5.32 |
| 13.999 | 2,4,6-Me3-Glc p | →3)-Glc p-(1→ | 2.85 |
| 14.133 | 2,3,6-Me3-Glc p | →4)-Glc p-(1→ | 3.79 |
| 14.461 | 2,3,4-Me3-Glcp | →6)-Glc p-(1→ | 1.54 |
| 15.853 | 2,3-Me2-Glc p | →4,6-Glc p-(1→ | 0.41 |
| 15.973 | 2,4-Me2-Glc p | →3,6-Glc p-(1→ | 1.00 |
Glucose residue chemical shift full ownership in 2 polysaccharide GLPPSQ of table
The HMBC spectrum analysis results of glucose residue in 3 polysaccharide GLPPSQ of table
It is connected from residue A shown in table 3 with the C-6 of residue E with C-4；Residue B is connected with the C-3 of residue B/C；Residue C and residue B
C-3 be connected；Residue D is connected with the C-6 of the C-4 and residue F of residue D；Residue E is connected with the C-4 of residue D；Residue F and residual
The C-6 of the C-6 and residue C of base F are connected.
The NOESY spectrum analysis results of glucose residue in 4 polysaccharide GLPPSQ of table
It is connected from residue A shown in table 4 with the C-6 of residue E with C-4；Residue D is connected with the C-6 of the C-4 and residue F of residue D；
Residue E is connected with the C-4 of residue D；Residue F is connected with the C-6 of the C-6 and residue C of residue F, this result is further verified
The analysis result of HMBC.
Active components of glossy ganoderma is inferred according to above-mentioned experimental result（GL-PPSQ）The repetitive unit of structure is：
Synthetic nucleus magnetic chart spectrum analysis, monose composition and methylation analysis, it is known that polysaccharide GLPPSQ is based on glucan.
Ganoderma polysaccharide peptide as described above：It is characterized in that content 24.58% of the log glossy ganoderma peptidoglycan by glucose, sweet dew
Sugar is 2.51%（See Figure 14）；The ganoderma lucidum monose of JUNCAO Ganoderma lucidum peptidoglycan or other Bag Materials is by the content 14.27% of glucose, sweet dew
Sugared content 2.91%, arabinose content 5.82% and Xylose Content 6.63% form（See Figure 15）.
Above-mentioned ganoderma active polysaccharide peptide isolation and purification method；（1）Ganodenna Lucidum P.E preparation solution and purification：Fructification is cut into slices
Or it is broken into filiform；2.0~4.0h of soak at room temperature, material and water ratio are 1: 10~20 times, and boiling water boiling 2 times is respectively every time
And filtrates are closed in 1.5h and 1.0h, filtering.Vacuum concentration is placed in, 65~68 DEG C of temperature, vacuum -0.09~-0.1MPa is concentrated under reduced pressure
It is lucidum extracting liquid to 10 times or so of fructification quality.
Above-mentioned preparation solution carries out purified treatment in the following manner：Such as centrifugation（Speed is 4000~6000r.min-1It is or high
Fast centrifuge（10000~12000r.min-1）, 10~15 points of time, removing coarse impurities；Then respectively with 5 μm of aperture, 1 μm,
0.45 μm, 0.1 μm of filtering with microporous membrane or use 750KD hollow fiber column ultrafilters.
（2）Ganoderma polysaccharide peptide extracts：The above-mentioned ganoderma lucidum scavenging solution after micro-filtration, using 10KD films or 10KD hollow fiber columns
Ultrafiltration retains the ultrafiltrate of more than 10KD molecular masses, and ultrasiltrated rate controls 1~10L.h-1Or 20~25L.h-1, operating temperature
For room temperature, 0.1~0.2Mpa of operating pressure, pump：Variable ratio frequency changer is at a high speed.Film bag surface or hollow fibre are continued to flow through with certain speed
Column is tieed up, is macromolecule ganoderma active polysaccharide peptide lucidum extracting liquid.
The present invention is implemented using Centramate ultrafiltration systems：Retention 1.0 × 104More than Da molecular weight is ganoderma lucidum polysaccharide
Peptide, since cultivating ganoderma raw material is different, actually molecular cut off slightly has difference, such as log glossy ganoderma extract, ganoderma lucidum polysaccharide
Peptide molecular weight is 1.4 × 104More than Da, JUNCAO Ganoderma lucidum extract, ganoderma lucidum polysaccharide molecular weight is 1.0 × 104More than Da.
The extraction of ganoderma polysaccharide peptide active site：It is characterized in that ganoderma lucidum aqueous extract（Embodiment 2 operates）Add in triploid
95% ethanol precipitation of product, is further purified, and is freeze-dried up to ganoderma polysaccharide peptide active component（GL-PP）Semifinished product yield 0.76%
（n=3）.
（3）The extraction of oral agents ganoderma polysaccharide peptide raw material：Using slipstream（TFF）Molecular cut off technology, concrete operations
It is as follows：Lucidum extracting liquid is passed through into micro-filtration（MF）, the aperture of film is at 0.1 micron（μm）To between 10 μm, sample introduction pressure（Control
0.68Mpa）：Temperature is room temperature, preliminary clearning, micro-filtration processing.Slipstream molecular cut off technical parameter：1. match the hollow fibres of 10KD
Column is tieed up, molecular cut off is 1.0 × 104More than Da, column specification：100~1400cm2；2. internal diameter of the pipeline, specification：3.2~
6.4mm；3. inlet pressure（Pf）, specification：0.00~2000bar；4. inlet velocity, 100~6000ml.min-1@4bar；5.
Return pressure（Pr）, 0.01~10.00bar；6. convey flow velocity, 20~1000ml.min-1@1bar；7. flow velocity is filtered out, 20~
1000ml.min-1@1bar；8.TMP, 0.3~4.0bar.By above-mentioned technical parameter, retention relative molecular mass is collected respectively
For 1.0 × 104The macromolecular position of more than Da is ganoderma polysaccharide peptide.By vacuum spray dehydrator, control inlet temperature 175~
180 DEG C, outlet temperature is 90~95 DEG C, and deep processing is made pale yellow powder shape extract or by fluid bed low temperature spray drying
Into pale yellow powder shape extract, it is characterized in that the ganoderma polysaccharide peptide semifinished product without apparent bitter taste.
（4）Ganoderma polysaccharide peptide detection method of content：It is detected with HPLC-ELSD methods, chromatographic condition is as follows：
1515 types of instrument Waters, evaporative light detector condition：Photodetector is evaporated using 2424 types of Waters；Drift tube temperature
55 DEG C of degree.It sprays as heating mode；Gas pressure is 45psi；Chromatographic column TSK G4000PWXLColumn；Mobile phase：Methanol: water=20:
80；Flow velocity：1ml/min ；Column temperature：35℃；Sample size 10ul；
Operating procedure：
1. Specification Curve of Increasing
Precision weighs dry peptidoglycan reference substance 0.0500g（Purity>95%, national Juncao Industry Technology Center）Hold in 10mL
In measuring bottle, with water dissolution and constant volume, concentration is obtained as 5.00mg.mL-1Peptidoglycan standard reserving solution；Before use, 100 μ are taken respectively
L, 200 μ L, 300 μ L, 400 μ L, 500 μ L, in 5mL volumetric flasks, be diluted with water to scale, obtain concentration be respectively 100.0,
200.0、300.0、400.0、500.0μg.mL-1Standard working solution, with reference substance concentration（μg /ml）Logarithm（X）For horizontal seat
Mark, peak area logarithm（Y）For ordinate, standard curve is drawn（See Figure 16）.
2. the preparation of sample solution
Ganoderma lucidum product 0.2g is weighed, water 8mL is added to extract 30min after 60 DEG C of water-baths, cooling is settled to 10mL；Filtering, takes filtrate
1mL adds 16mL absolute ethyl alcohols, mixing.15min is centrifuged in 10000rpm, precipitation mobile phase 2ml dissolves, and 0.22 μm has machine filter
It is spare to obtain test sample filtrate for membrane filtration.
2. analysis result represents:
The not described part of the present embodiment is same as the prior art.
Embodiment 2
The step of ganoderma active polysaccharide peptide product of the present invention is made oral liquid sees Figure 17.
The HPLC figures of ganoderma polysaccharide peptide in Fig. 1 log glossy ganoderma extracting solutions.Chromatographic condition：Instrument：High performance liquid chromatograph
Waters 1515；2424 evaporation photodetectors, sample introduction：10ul, column temperature：35 DEG C, TSK G5000PWXLWith TSK G3000PWXL
Column string takes detection.High-molecular compound main peak content 18.38%, area 49.23%；Low molecular compound main peak content 44.79%,
Area 50.77% adds up to area 100%.
The HPLC figures of ganoderma polysaccharide peptide in Fig. 2 JUNCAO Ganoderma lucidum extracting solutions.Chromatographic condition：Instrument：High performance liquid chromatograph Waters
1515；2424 evaporation photodetectors, sample introduction：10ul, column temperature：35 DEG C, TSK G5000PWXLWith TSK G3000PWXLColumn string takes inspection
It surveys.High-molecular compound main peak content 22.77%, area 59.68%；Low molecular compound main peak content 27.82%, area
40.33%, add up to area 100.01%.
Fig. 3 is JUNCAO Ganoderma lucidum extract molecular weight determination（Molecular weight is more than more than 10KD）.Chromatographic condition：Instrument：Efficiently
Liquid chromatograph Waters, 2414 Composition distributions, sample introduction：10ul, column temperature：35 DEG C, column：TSK G4000PWXL.Retention time
8.093 points, Mp2.8 × 104, area 94.91%.
Fig. 4 log glossy ganoderma extract molecular weight determinations（Molecular weight is more than more than 10KD）.Chromatographic condition：Instrument：Efficient liquid
Chromatography Waters, 2414 Composition distributions, sample introduction：10ul, column temperature：35 DEG C, column：TSK G4000PWXL.Retention time
7.958 points, Mp2.6 × 104, area 76.12%.
Fig. 5 JUNCAO Ganoderma lucidums fine powder dialysis retention high score sub-portion.Chromatographic condition：Instrument：High performance liquid chromatograph Waters,
2414 Composition distributions, sample introduction：10ul, column temperature：35 DEG C, column：TSK G4000PWXL.Retention time 8.693 is divided, and Mp1.1 ×
104, area 56.50%.
Fig. 6 ganoderma polysaccharide peptide GL-PPSQ amino acid collection of illustrative plates.Sample is on L-8900 Hitachis automatic amino acid analyzer
Measurement result.
Fig. 7 ganoderma polysaccharide peptides GLPPSQ1H-NMR collection of illustrative plates：1H NMR mainly solve the glucoside key that monose forms in polysaccharide structures
Configuration problem, the signal majority of polysaccharide is in δ 4.0~5.5ppm scopes, general α types pyranose C1The δ values of upper proton are more than
5.0ppm, and β-type is less than 5.0ppm.
Fig. 8 ganoderma polysaccharide peptides GLPPSQ13C-NMR collection of illustrative plates：13Up to 200ppm, it can not only be true for the chemical shift of C NMR
The position of fixed various carbon, and the type and conformation of monose can also be distinguished.
According to ganoderma polysaccharide peptide GL-PPSQ1H-NMR and13C-NMR collection of illustrative plates, the contrast table that reads up the literature GL-PPSQ recurring units are
Main chain is by glucose group into -4) Glcp(1-, -3) Glcp(1- and -6) Glcp(main chain that 1- is formed, -6) Glcp(4 of 1-
Shang You branches connect an Xylp。
Fig. 9 heteronuclear Multiple-quantum Correlated Spectroscopy CHMQC collection of illustrative plates：Hydrocarbon correlation figure-total correlation figure and same core two-dimensional spectrum（COSY）
Figure.
Figure 10 HSQC-TOCSY（Hydrocarbon correlation figure-total correlation figure）：It can be looked in the HSQC collection of illustrative plates of polysaccharide GLPPSQ samples
Go out four stronger anomer hydrogen carbon phase OFF signal peak 4.53/105.21,4.53/105.21,5.00/100.45 and
4.79/105.21 ppm shows that polysaccharide at least there are four types of the residue of various configuration, is denoted as A, B, D and E.
The COSY collection of illustrative plates of Figure 11 GLPPSQ（Same core two-dimensional spectrum) (1H-1H）：COSY can find out matter adjacent in saccharide residue
Son, and HSQC-TOCSY can also further determine that the hydrocarbon signal in identical saccharide residue.
The HMBC collection of illustrative plates of Figure 12 GL-PPSQ（The long-range related collection of illustrative plates of hydrogen and carbon）：HMBC can obtain the connection position of glycosidic bond
It puts.
The NOESY collection of illustrative plates of Figure 13 GL-PPSQ（The related figure of space hydrogen hydrogen）：It can be obtained by space HH correlations NOESY spectrums
The link position of glycosidic bond
Figure 14 log glossy ganoderma peptidoglycans monose forms（Molecular weight ranges ＞ 10KD）.Chromatographic condition：High performance liquid chromatograph (
1515 infusion pumps of waters, 2424 evaporation photodetectors, 2414 Composition distributions), waters chromatographic work stations, chromatographic column:
TSK G4000PWXL；4.6 × 250mm of ACQUITY BEH Amide chromatographic columns.
Figure 15 JUNCAO Ganoderma lucidum peptidoglycans monose forms（Molecular weight ranges ＞ 10KD）.Chromatographic condition：High performance liquid chromatograph
(1515 infusion pumps of waters, 2424 evaporation photodetectors, 2414 Composition distributions), waters chromatographic work stations, chromatographic column:
TSK G4000PWXL；4.6 × 250mm of ACQUITY BEH Amide chromatographic columns.
Figure 16 ganoderma polysaccharide peptide content detection canonical plottings.
Figure 17 ganoderma polysaccharide peptide oral liquid process flow charts.
Claims (6)
1. a kind of active components of glossy ganoderma peptidoglycan, it is characterised in that：The peak molecular weight M of the ganoderma active polysaccharide peptidePFor
1.0×104The ganoderma polysaccharide peptide high-molecular compound of more than Da.
2. active components of glossy ganoderma peptidoglycan according to claim 1, it is characterised in that：The ganoderma polysaccharide peptide macromolecule of gained
Compound is by two or more different monose and 17 kinds of Amino acid profiles.
3. active components of glossy ganoderma peptidoglycan according to claim 2, it is characterised in that：Ganoderma polysaccharide peptide is by glucose and sweet
Dew sugar composition；Its molar ratio 31.85: 1.00.
4. active components of glossy ganoderma peptidoglycan according to claim 3, it is characterised in that：The ganoderma polysaccharide peptide macromolecule
Compound based on glucan, the structural repeat unit main chain of dextran portion by
。
5. a kind of active components of glossy ganoderma peptidoglycan reference substance, it is characterised in that：By the ganoderma polysaccharide peptide macromolecule of claim 1
Compound is classified alcohol precipitation through GL-PP, and Bio-Gl, P-10 pillar layer separations obtain ganoderma active polysaccharide peptide GL-PPSQ reference substances, pure
It spends for more than 97%, it is 6.25 × 10 to divide position molecular weight4 Da。
6. a kind of production method of active components of glossy ganoderma peptidoglycan described in claim 1, it is characterised in that：By purified spirit
Sesame extracting solution uses 10KD UF membranes or bag filter, molecular cut off MP1.0×104More than Da, gained are without bitter taste ganoderma lucidum
Peptidoglycan high-molecular compound.
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| CN111671764A (en) * | 2020-05-06 | 2020-09-18 | 广东华夏友美生物科技有限公司 | Application of ganoderic acid polysaccharide in preparation of skin histamine inhibitor |
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