CN108041173A - A kind of pure Semen Coicis acidified milk - Google Patents

A kind of pure Semen Coicis acidified milk Download PDF

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Publication number
CN108041173A
CN108041173A CN201810003088.0A CN201810003088A CN108041173A CN 108041173 A CN108041173 A CN 108041173A CN 201810003088 A CN201810003088 A CN 201810003088A CN 108041173 A CN108041173 A CN 108041173A
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China
Prior art keywords
semen coicis
lactobacillus
dextrin
seed liquor
hours
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Pending
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CN201810003088.0A
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Chinese (zh)
Inventor
丁筑红
张洪礼
柳飞
彭邦远
刘敏
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Guizhou University
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Guizhou University
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Priority to CN201810003088.0A priority Critical patent/CN108041173A/en
Publication of CN108041173A publication Critical patent/CN108041173A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C11/00Milk substitutes, e.g. coffee whitener compositions
    • A23C11/02Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins
    • A23C11/10Milk substitutes, e.g. coffee whitener compositions containing at least one non-milk component as source of fats or proteins containing or not lactose but no other milk components as source of fats, carbohydrates or proteins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/125Casei
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/167Pentosus

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A kind of pure Semen Coicis acidified milk, comprises the following steps:1)Semen Coicis feedstock processing;2)The preparation of Semen Coicis dextrin:Semen Coicis breast is prepared with 9% coixlacrymajobi powder, 7% sucrose, is gelatinized in boiling water bath;3)The preparation of Rhizopus oryzae seed liquor:The disposable bacteria leaven spore that feeds intake of access 1% in seed culture medium, constant-temperature shaking culture for 24 hours, obtains Rhizopus oryzae seed liquor in 30 DEG C, 120 r/min shaking tables;4)The preparation of lactobacillus solution:By Lactobacillus casei and Lactobacillus pentosus respectively with 3%(V/V)Inoculum concentration be inoculated into MRS culture mediums, the seed liquor of Lactobacillus casei and Lactobacillus pentosus is made;5)Semen Coicis dextrin ferments:Rhizopus oryzae seed liquor is added to 2% in gelatinization Semen Coicis liquid, ferment 48h at 35 DEG C, adds in 2% immediately(V/V)Lactobacillus pentosus and 2%(V/V)Lactobacillus casei, in 35 DEG C fermentation for 24 hours, obtain fermentation Semen Coicis breast.The nutrition of raw material and product and function quality can not only be improved, be also obviously improved the flavor quality of product.

Description

A kind of pure Semen Coicis acidified milk
Technical field
The invention belongs to food processing field, more particularly to a kind of pure Semen Coicis acidified milk and its processing method.
Background technology
Semen Coicis is one of important rain fed crops in China, full of nutrition, ingredient containing plurality kinds of health care and anticancer active constituent, Semen Coicis has hypoglycemic, antitumor, inhibition obesity, improves immunity, easing pain and diminishing inflammation and inhibit osteoporosis and other effects, is curing It is widely used in medicine, health food and ordinary food.Since Semen Coicis flavor is flat, grease easily occurs in processing and storage It aoxidizes and causes product deterioration, content of starch is higher and not easy to be processed and the problems such as easily sending out aging farinose, causes product product The bad Shelf-life Quality of matter is unstable, makes Semen Coicis product Related product less, mainly with Semen Coicis raw material, anticancer active constituent(Health The heart of a lotus seed coix seed oil of Lai Te)And based on normal conventional food etc., Semen Coicis industry development is constrained.
Macromolecular substances in Semen Coicis can be carried out by effective decomposition and inversion by fermented foodstuff, have and promote product quality wind The function of taste, but Semen Coicis acidified milk method mainly uses lactic acid bacteria, monascus specie at present, with Semen Coicis combination cow's milk or other auxiliary materials Mixed fermentation is carried out, fermenting microbe is more single, and flavor quality is not good enough, and Semen Coicis raw material availability and quality heterosis fail abundant table It is existing.
The content of the invention
It is an object of the invention to provide a kind of pure Semen Coicis acidified milk and its processing methods, it uses pure Semen Coicis as raw material, It is sufficiently reserved and using Semen Coicis nutritional ingredient, using Rhizopus oryzae combination Lactobacillus casei and Lactobacillus pentosus, passes through step fermentation. This method is compared compared with currently available technology, can not only be improved the nutrition of raw material and product and function quality, has also been obviously improved production The flavor quality of product.At present, same or similar patent and technology have not been reported.
The purpose of the present invention and its technical problem underlying is solved using following technical scheme to realize:The present invention relates to A kind of pure Semen Coicis acidified milk, it is process by following processing step:
1)Semen Coicis feedstock processing:Selection is crushed without rotten and impurity myotonin and crosses 100 mesh sieves, it is standby that coixlacrymajobi powder is made With;
2)The preparation of Semen Coicis dextrin:Semen Coicis whey solution is prepared with 9% coixlacrymajobi powder, 7% sucrose, 30min is gelatinized in boiling water bath, it is cold But to room temperature, Semen Coicis dextrin is obtained;
3)The preparation of Rhizopus oryzae seed liquor:The disposable bacteria leaven spore that feeds intake of access 1% in seed culture medium, 30 DEG C, constant-temperature shaking culture for 24 hours, obtains Rhizopus oryzae seed liquor in 120 r/min shaking tables;The seed culture medium is the potato of 20 % Diffusion juice and 2 % glucose;
4)The preparation of lactobacillus solution:By Lactobacillus casei and Lactobacillus pentosus respectively using volume ratio V/V as 3% inoculum concentration MRS culture mediums are inoculated into, condition of culture is 36 ± 1 DEG C, reaches exponential phase i.e. viable count > 10 with interior for 24 hours6During cfu/mL Stop;By the Lactobacillus casei activated and Lactobacillus pentosus respectively in Semen Coicis dextrin and MRS culture volumes than 0:10,1: 9,2:8,3:7,4:6,5:5,6:4,7:3,8:2,9:1,10:It is tamed successively in 0 mixed culture medium, makes bacterial strain in 100% sample liquid It can be good at growth until viable count > 106cfu/mL;Lactobacillus casei by domestication is with Lactobacillus pentosus respectively with 5% It ferments in the Semen Coicis dextrin of addition for 24 hours, the seed liquor of Lactobacillus casei and Lactobacillus pentosus is made;
5)Semen Coicis dextrin ferments:Rhizopus oryzae seed liquor is added to 2% in gelatinization Semen Coicis liquid, ferment 48h at 35 DEG C, with The Lactobacillus pentosus that volume ratio V/V is 2% and the Lactobacillus casei that volume ratio V/V is 2% are added in, in 35 DEG C of fermentations for 24 hours, is obtained To fermentation Semen Coicis breast.
A kind of above-mentioned pure Semen Coicis acidified milk, step 5)The sterilization of seasoning bottling afterwards:The Semen Coicis fermented breast is added in 7% Sucrose, dissolving bottling sealing, 30min is sterilized at 75 DEG C, and must ferment Semen Coicis breast.
Patent of the present invention has clear advantage and advantageous effect compared with prior art.From above technical scheme, The present invention use pure Semen Coicis as raw material, is sufficiently reserved and using Semen Coicis nutritional ingredient, using Rhizopus oryzae combination Lactobacillus casei with Lactobacillus pentosus by step fermentation, can not only improve the nutrition of raw material and product and function quality, also be obviously improved product Flavor quality expanded the application function of strain used simultaneously.
Description of the drawings
Fig. 1 is the process flow chart of the present invention.
Specific embodiment
Below in conjunction with attached drawing and preferred embodiment, to according to a kind of pure Semen Coicis acidified milk specific embodiment party proposed by the present invention Formula, feature and its effect are described in detail as after.
Referring to Fig. 1, a kind of pure Semen Coicis acidified milk, it is process by following processing step:
1)Semen Coicis feedstock processing:Selection is crushed without rotten and impurity myotonin and crosses 100 mesh sieves, it is standby that coixlacrymajobi powder is made With;
2)The preparation of Semen Coicis dextrin:Semen Coicis whey solution is prepared with 9% coixlacrymajobi powder, 7% sucrose, 30min is gelatinized in boiling water bath, it is cold But to room temperature, Semen Coicis dextrin is obtained;
3)The preparation of Rhizopus oryzae seed liquor:The disposable bacteria leaven spore that feeds intake of access 1% in seed culture medium, 30 DEG C, constant-temperature shaking culture for 24 hours, obtains Rhizopus oryzae seed liquor in 120 r/min shaking tables;The seed culture medium is the potato of 20 % Diffusion juice and 2 % glucose;
4)The preparation of lactobacillus solution:By Lactobacillus casei and Lactobacillus pentosus respectively using volume ratio V/V as 3% inoculum concentration MRS culture mediums are inoculated into, condition of culture is 36 ± 1 DEG C, reaches exponential phase i.e. viable count > 10 with interior for 24 hours6During cfu/mL Stop;By the Lactobacillus casei activated and Lactobacillus pentosus respectively in Semen Coicis dextrin and MRS culture volumes than 0:10,1: 9,2:8,3:7,4:6,5:5,6:4,7:3,8:2,9:1,10:It is tamed successively in 0 mixed culture medium, makes bacterial strain in 100% sample liquid It can be good at growth until viable count > 106cfu/mL;Lactobacillus casei by domestication is with Lactobacillus pentosus respectively with 5% It ferments in the Semen Coicis dextrin of addition for 24 hours, the seed liquor of Lactobacillus casei and Lactobacillus pentosus is made;
5)Semen Coicis dextrin ferments:Rhizopus oryzae seed liquor is added to 2% in gelatinization Semen Coicis liquid, ferment 48h at 35 DEG C, with The Lactobacillus pentosus that volume ratio V/V is 2% and the Lactobacillus casei that volume ratio V/V is 2% are added in, in 35 DEG C of fermentations for 24 hours, is obtained To fermentation Semen Coicis breast.
A kind of above-mentioned pure Semen Coicis acidified milk, step 5)The sterilization of seasoning bottling afterwards:The Semen Coicis fermented breast is added in 7% Sucrose, dissolving bottling sealing, 30min is sterilized at 75 DEG C, and must ferment Semen Coicis breast.
Beyond above-mentioned percent composition, not do the component that illustrates be water.
Product of the present invention carries out quality evaluation with related process product:To using not fermenting, Rhizopus oryzae fermentation, lactic acid bacteria sends out The product of ferment, one-step fermentation and the present invention carry out analysis comparison, and the result is shown in tables 1.
As seen from Table 1:
1st, the flavor of different technologies product and flavour difference are larger, and with the product special flavour performance optimal that the present invention is fermented, sense organ is commented Valency result is optimal, the lactic fermentation feature flavour for having strong fermentation fragrant and sweet and mellow.
2nd, the more other technology product quality of product nutritional ingredient of the present invention are compared, and are obviously improved, wherein having Machine acid is improved to be promoted beneficial to flavor and nutritional quality, and particularly Semen Coicis feature functionality factor glycerol trioleate is than other technologies work Skill product is increased up to 12.5 times, and amino-acid nitrogen, triterpene and total phenol content promote nearly 2 times than other handicraft products, POV values Show that oxidation stability is good.
3rd, Semen Coicis breast using the present invention not only has strong ferment local-flavor and quality stability, further increases battalion Healthcare function component content is supported, the nutrition and health care quality characteristic of product is promoted while flavor is improved.Effectively enhancing Semen Coicis money The processing quality in source enriches fermentation Semen Coicis product variety, improves its market competition advantage, while has expanded answering for strain used Use function.By verifying repeatedly, determine that the technical application is feasible, more existing relative technological products comparative advantages are apparent, and Semen Coicis Resource, which is processed and utilized into one, provides Technology origin.
The above is only the preferred embodiment of patent of the present invention, and not patent of the present invention is made in any form Limitation, any without departing from art solutions content of the present invention, the technical spirit according to patent of the present invention is to above example Any simple modification, equivalent change and modification made, in the range of still falling within art solutions of the present invention.

Claims (2)

1. a kind of pure Semen Coicis acidified milk, it is characterised in that:It is process by following processing step:
1)Semen Coicis feedstock processing:Selection is crushed without rotten and impurity myotonin and crosses 100 mesh sieves, it is standby that coixlacrymajobi powder is made With;
2)The preparation of Semen Coicis dextrin:Semen Coicis whey solution is prepared with 9% coixlacrymajobi powder, 7% sucrose, 30min is gelatinized in boiling water bath, it is cold But to room temperature, Semen Coicis dextrin is obtained;
3)The preparation of Rhizopus oryzae seed liquor:The disposable bacteria leaven spore that feeds intake of access 1% in seed culture medium, 30 DEG C, constant-temperature shaking culture for 24 hours, obtains Rhizopus oryzae seed liquor in 120 r/min shaking tables;The seed culture medium is the potato of 20 % Diffusion juice and 2 % glucose;
4)The preparation of lactobacillus solution:By Lactobacillus casei and Lactobacillus pentosus respectively using volume ratio V/V as 3% inoculum concentration MRS culture mediums are inoculated into, condition of culture is 36 ± 1 DEG C, reaches exponential phase i.e. viable count > 10 with interior for 24 hours6During cfu/mL Stop;By the Lactobacillus casei activated and Lactobacillus pentosus respectively in Semen Coicis dextrin and MRS culture volumes than 0:10,1: 9,2:8,3:7,4:6,5:5,6:4,7:3,8:2,9:1,10:It is tamed successively in 0 mixed culture medium, makes bacterial strain in 100% sample liquid It can be good at growth until viable count > 106cfu/mL;Lactobacillus casei by domestication is with Lactobacillus pentosus respectively with 5% It ferments in the Semen Coicis dextrin of addition for 24 hours, the seed liquor of Lactobacillus casei and Lactobacillus pentosus is made;
5)Semen Coicis dextrin ferments:Rhizopus oryzae seed liquor is added to 2% in gelatinization Semen Coicis liquid, ferment 48h at 35 DEG C, with The Lactobacillus pentosus that volume ratio V/V is 2% and the Lactobacillus casei that volume ratio V/V is 2% are added in, in 35 DEG C of fermentations for 24 hours, is obtained To fermentation Semen Coicis breast.
2. a kind of pure Semen Coicis acidified milk as described in claim 1, it is characterised in that:Step 5)The sterilization of seasoning bottling afterwards:It will The Semen Coicis breast fermented adds in 7% sucrose, and dissolving bottling sealing sterilizes 30min at 75 DEG C, and must ferment Semen Coicis breast.
CN201810003088.0A 2018-01-02 2018-01-02 A kind of pure Semen Coicis acidified milk Pending CN108041173A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111493146A (en) * 2020-04-22 2020-08-07 广西壮族自治区农业科学院 Preparation method of jasmine-flavored persimmon fermented milk
CN115053955A (en) * 2022-06-07 2022-09-16 聚慧食品科技(重庆)有限公司 Corn fermented sauce based on composite bacteria segmented fermentation and preparation method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101828603A (en) * 2010-05-19 2010-09-15 白城师范学院 Pure plant-fermented yogurt
CN102894443A (en) * 2012-09-19 2013-01-30 暨南大学 Coix seed beverage and preparation method thereof
CN103416755A (en) * 2013-08-28 2013-12-04 贵州大学 Walnut meal composite nutrition powder and preparation technology thereof
CN104170957A (en) * 2014-08-14 2014-12-03 安徽燕之坊食品有限公司 Coix seed fermented beverage and preparation method thereof
CN104304450A (en) * 2014-10-09 2015-01-28 哈尔滨工业大学 Semen coicis lactic acid bacteria beverage fermentation process

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101828603A (en) * 2010-05-19 2010-09-15 白城师范学院 Pure plant-fermented yogurt
CN102894443A (en) * 2012-09-19 2013-01-30 暨南大学 Coix seed beverage and preparation method thereof
CN103416755A (en) * 2013-08-28 2013-12-04 贵州大学 Walnut meal composite nutrition powder and preparation technology thereof
CN104170957A (en) * 2014-08-14 2014-12-03 安徽燕之坊食品有限公司 Coix seed fermented beverage and preparation method thereof
CN104304450A (en) * 2014-10-09 2015-01-28 哈尔滨工业大学 Semen coicis lactic acid bacteria beverage fermentation process

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111493146A (en) * 2020-04-22 2020-08-07 广西壮族自治区农业科学院 Preparation method of jasmine-flavored persimmon fermented milk
CN115053955A (en) * 2022-06-07 2022-09-16 聚慧食品科技(重庆)有限公司 Corn fermented sauce based on composite bacteria segmented fermentation and preparation method thereof

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Application publication date: 20180518