CN108034611A - One bacillus amyloliquefaciens and its application - Google Patents
One bacillus amyloliquefaciens and its application Download PDFInfo
- Publication number
- CN108034611A CN108034611A CN201810029297.2A CN201810029297A CN108034611A CN 108034611 A CN108034611 A CN 108034611A CN 201810029297 A CN201810029297 A CN 201810029297A CN 108034611 A CN108034611 A CN 108034611A
- Authority
- CN
- China
- Prior art keywords
- bacillus amyloliquefaciens
- asparagus
- application
- fungal diseases
- plants
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Dentistry (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- General Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to biological technical field, and in particular to a bacillus amyloliquefaciens and its application.Adopt the technical scheme that：Screening isolates one plant of bacillus amyloliquefaciens MY001,2017 with chitin degrading ability and was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, strain name July 26 from the soil of plantation asparagus:Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MY001., deposit number are：CGMCC NO.14460.The bacterial strain is prevented available for Asparagus Stem Blight, and specifically used method is：It is 1 × 10 by concentration7The bacillus amyloliquefaciens MY001 microbial inoculums of cfu/mL carry out 500~1000 times of dilutions, sprayed respectively in sprout early period, asparagus pumping phase and normal growth phase of asparagus, frequency is sprayed to be spaced 5~10 days 1 time, is sprayed altogether 5~10 times, spraying time is no more than 60 days.The bacterial strain is of great significance for prevention Asparagus Stem Blight.
Description
Technical field
The invention belongs to biological technical field, and in particular to a bacillus amyloliquefaciens and its application.
Background technology
" cancer " of Asparagus Stem Blight (the Asparagus stem blight) asparagus that is otherwise known as, its disease symptom are main
Stem, side shoot or leaf are concentrated on, has the characteristics that rapid onset, infectivity, loss are serious, once there is asparagus infection, it is easy to big face
Product is propagated, and causes full wafer Asparagus Plants to turn yellow suddenly withered, it is impossible to throughput asparagus product.Its principal causative pathogen is day
Door winter Phomopsis (Phomopsis asparagi).At present to the prevention of Asparagus Stem Blight, mainly using carbendazim, Bravo
Deng chemical pesticide, but long-time service such chemical pesticide, being easy to cause germ develops immunity to drugs, can not be constantly withered to asparagus stem
Disease is effectively prevented.Therefore, it is the feasible method for preventing Asparagus Stem Blight to develop effective biological pesticide.
Bacillus is distributed widely in nature, including bacillus subtilis, bacillus licheniformis, bacillus cereus
Deng these bacterial strains are strong to extraneous injurious factor resistance.A variety of bacillus, which have, suppresses pathogen of crop flourish
Effect, be good biocontrol bacteria.
Bacillus amyloliquefaciens (B.amyloliquefaciens) belong to gram-positive bacteria, it has been found that, solve starch bud
Spore bacillus has phytopathogen broad-spectrum antibacterial, but its antibacterial validity still has extremely strong specificity, for difference
Pathogen need to filter out special biocontrol bacterial strain and can be only achieved optimal fungistatic effect.And bacillus amyloliquefaciens are applied to
The research of fungal diseases of plants prevention, has focused largely on the crops such as strawberry, tomato, peach, potato, and specificity prevention asparagus stem is withered
There is not been reported for the bacillus amyloliquefaciens of disease.
The content of the invention
The object of the present invention is to provide a bacillus amyloliquefaciens and its application.
For achieving the above object, the technical solution adopted in the present invention is：One bacillus amyloliquefaciens, 2017
It was preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on July 26, preservation address is BeiJing, China, bacterium
Kind title:Bacillus amyloliquefaciens, Bacillus amyloliquefaciens, MY001., deposit number are：CGMCC
NO.14460。
Correspondingly, its 16SrDNA sequencing sequence is as shown in SEQ ID NO 1, its gyrA sequencing sequence such as SEQ ID NO 2
Shown, its gyrB sequencing sequence is as shown in SEQ ID NO 3.
Correspondingly, application of the bacillus amyloliquefaciens in fungal diseases of plants prevention.
Preferably, application of the bacillus amyloliquefaciens in fungal diseases of plants prevention, the fungi include：Tianmen
Winter Phomopsis bacterium, intend Pestalotia bacterium, Curvularia bacterium.
Preferably, application of the bacillus amyloliquefaciens in Asparagus Stem Blight.
Preferably, the bacillus amyloliquefaciens are for the application method for preventing Asparagus Stem Blight：Starch gemma will be solved
Bacillus MY001 microbial inoculums dilute 500~1000 times, are sprayed respectively in sprout early period, asparagus pumping phase and normal growth phase of asparagus
Apply, spray frequency to be spaced 5~10 days 1 time, spray altogether 5~10 times, whole spraying time is no more than 60 days.
Correspondingly, application of the bacillus amyloliquefaciens in fungal diseases of plants prevention, including by the solution starch
Bacillus prevents for active ingredient applied to fungal diseases of plants, also includes being applied to plant using its metabolite as active ingredient
Thing fungal disease is prevented.
The invention has the advantages that：One plant of new solution starch bud with chitin degrading ability is isolated in screening
The fungies such as spore bacillus, MY001, pathogen lucid asparagus Phomopsis bacterium of the bacterial strain to causing Asparagus Stem Blight have excellent
Inhibitory action, for prevention Asparagus Stem Blight have important practical significance.
Brief description of the drawings
Fig. 1 is the hydrolysis that aimed strain is formed；
Fig. 2 is the systematic evolution tree that aimed strain is compared with the 16SrDNA of bacillus type strain；
Fig. 3 is the systematic evolution tree that the gyrA genes of sequencing result and type strain are carried out to Multiple Sequence Alignment；
Fig. 4 is the systematic evolution tree that the gyrB genes of sequencing result and type strain are carried out to Multiple Sequence Alignment；
Fig. 5 is inhibition comparison diagram of the aimed strain to lucid asparagus Phomopsis bacterium；
Fig. 6 is inhibition comparison diagram of the aimed strain to plan Pestalotia bacterial strain；
Fig. 7 is inhibition comparison diagram of the aimed strain to Curvularia bacterial strain.
Embodiment
Embodiment one：The screening of bacterial strain
1st, collecting soil sample：Soil sample is gathered from Mianyang planting asparagus area.
2nd, culture medium is prepared：
(1) chitosan enzyme screening and culturing medium (g/L)：Colloid chitosan 2.0, K2HPO40.7, KH2PO40.3, (NH4)2SO4 5,MgSO4·7H2O 0.5, FeSO4·7H2O 0.01, agar 12.0, pH=7.0.
(2) broth bouillon (g/L)：Peptone 10.0, beef extract 3.0, NaCl 5.0, pH=7.0.
3rd, reagent preparation：
Prepare colloid chitosan:Fine powder chitosan 10g is weighed, adds appropriate concentrated hydrochloric acid, and 4 are stirred on magnetic stirring apparatus
~6h makes it fully dissolve, then cleans centrifugation (4000r/min) repeatedly with deionized water, eventually forms jelly, then on one side
Stirring adjusts pH to neutrality while being slowly added to NaOH, that is, obtains colloid chitosan, saved backup in 4 DEG C.
3rd, the primary dcreening operation of bacterial strain
(1) 5g soil samples are weighed, are put into the conical flask (250mL) for filling 45mL distilled water, stirring and dissolving, boils 30min,
Then supernatant is taken to carry out gradient dilution (10-1,10-2,10-3,10-4,10-5Totally 5 concentration), respectively take 100ul to be coated on shell and gather
In carbohydrase screening and culturing medium (each gradient setting 2 is parallel), 2~4d of culture in 37 DEG C of constant incubators is placed in.
(2) picking on selection culture plate growth and around have the bacterium colony of obvious hydrolysis, streak inoculation is in new
Chitosan enzyme screening and culturing medium on, be placed in 37 DEG C of constant incubators and cultivate 2d, then choose bacterium colony streak inoculation, so carry out 3
Generation screening, obtains purebred bacterial strain, is aimed strain.
(3) the aimed strain single bacterium that picking step (2) obtains is fallen within broth bouillon, carries out Liquid Culture.Then take
5ul nutrient solutions are placed on the filter paper of diameter 6mm, and filter paper is placed on chitosan enzyme screening and culturing medium, 37 DEG C of constant temperature incubation 2d,
Measure the size of hydrolysis.The hydrolysis of formation is shown in Fig. 1, and the hydrolysis size that aimed strain produces is shown in Table 1.
The hydrolysis size that 1 aimed strain of table produces
(4) aimed strain is purified, conservation, is stored under the conditions of -80 DEG C.
Embodiment two：Bacterial strain is identified
1st, 16SrDNA sequencing identifications
The genomic DNA of aimed strain is extracted, with the universal primer (27F of bacterial 16 S rDNA:AGA GTT TGA TCC
TGG CTC AG；1492R:TAC GGT TAC CTT GTT ACG ACT T) 16SrDNA is cloned from genomic DNA.PCR
As a result the sequencing of Hai Sheng works biotech firm is served, by sequencing result in Gene Bank website (http://www.nicbi.nlm.nih
.gov/ BLAST contrasts) are carried out, finds and downloads the gene order of reference culture, different bacterium are calculated using 7.0 softwares of MEGA
Genetic distance between strain, draws the phylogenetic tree of bacterium.16SrDNA sequencing results are as shown in SEQ ID NO 1.
Aimed strain is compared with the 16SrDNA of bacillus type strain, using MEGA7.0 build system into
Change tree, as shown in figure 3, wherein bacterial strain d1 and bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and withered grass bud
The genetic distance of spore bacillus (Bacillus subtilis) is nearest, is respectively:0.003 and 0.009.
2nd, gyrA and gyrB sequencing identifications
The A subunits and B subunits of gyrase, as special albumen, are guarded, every about 1,000,000 years gene evolutions in evolution
0.7~0.8%, 50,000,000 years more every than 16SrDNA evolve 1% speed faster, so it be usually used in identify affiliation closer to,
The bacterial strain that can not be distinguished with 16SrDNA.Using the genomic DNA of aimed strain as template, gyrase A and the B base of bacterium is expanded
Cause.
(1) gyrA is universal primer, gyrA-F：5’-CAG TCA GGA AAT GCG TAC GTC CTT-3’；gyrA-
R：5’-CAA GGT AAT GCT CCA GGC ATT GCT-3’.
Amplified production is sequenced, gyrA sequencing results are as shown in SEQ ID NO 2.By sequencing result and type strain
GyrA genes carry out Multiple Sequence Alignment, using MEGA7.0 build systematic evolution tree, as shown in Figure 4.Wherein strain to be tested with
The genetic distance of Bacillus amyloliquefaciens is nearest, be 0.053 (with the genetic distances of other bacterial strains 0.2 with
On).
(2) according to 16SrDNA qualification results, according to the conserved region of the gyrB sequences of several sibling specieses, drawing for gyrB is designed
Thing, i.e. gyrB-F：5’-AAC AGC AAA GGC CTT CAC CA-3’； gyrB-R：5’-GCA GAG TCA CCC TCT
ACG ATA TA-3’。
Amplified production is sequenced, gyrB sequencing results are as shown in SEQ ID NO 3.By sequencing result and type strain
GyrB genes carry out Multiple Sequence Alignment, using MEGA7.0 structure systematic evolution tree it is as shown in Figure 5.Wherein d1 and Bacillus
The genetic distance of amyloliquefaciens is nearest, is 0.045 (with the genetic distances of other bacterial strains more than 0.2).
3rd, based on the above results, it is bacillus amyloliquefaciens (Bacillus amyloliquefacien to determine aimed strain
s)。
The bacterial strain is on July 26th, 2017, in China Committee for Culture Collection of Microorganisms's common micro-organisms center
Preservation, strain name:Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) MY001., deposit number：CGMCC
NO.14460。
Embodiment three：Suppress the laboratory test of Asparagus Stem Blight pathogen
1st, pathogen is prepared：Isolated lucid asparagus Phomopsis, plan Pestalotia from Asparagus Stem Blight diseased plant
(Pestalotiopsis) bacterial strain, Curvularia (Curvularia) bacterial strain.
2nd, PDA culture medium (g/L) is prepared:200g peeled potatoes are cut into small pieces, boil 30min, are filtered off with gauze residual
Slag, adds glucose 20.0g/L, and agar 15.0g/L, pH are natural.
3rd, above-mentioned three classes pathogenic strains are inoculated in PDA culture medium center, are cultivated respectively in 28 DEG C of constant incubator
To a diameter of 3~4cm of fungus colony, then respectively bacterium colony four direction and be respectively labeled as at colony edge 0.5cm
1,2,3,4.Wherein 1,3 sites are control, are inoculated with 168 bacterial strain of bacillus subtilis, Bacillus subtilis 168
Strain, purchased from German Culture Collection Center DSMZ, strain number DSM 23778；2,4 sites are inoculated with bacillus amyloliquefaciens
(Bacillus amyloliquefaciens)MY001.Handle well be placed in 28 DEG C of constant incubator be inverted culture 3~
4 days, observe growth of pathogenic bacteria situation.
4th, antibacterial result is respectively as shown in Fig. 5~7.In inoculation the 3rd~4 day, control strain was covered by fungi, and
MY001 bacterial strains have then been respectively formed obvious inhibition zone to above-mentioned three classes pathogen.Show bacillus amyloliquefaciens MY001 to reed
Bamboo shoot stem wilt pathogen has extremely strong inhibitory action, the prevention available for Asparagus Stem Blight disease.
Example IV：Suppress the field trial of asparagus fungal pathogens
Field trial sets 3 processing altogether:A. bacillus amyloliquefaciens MY001 suspensions, effective bacterial content are 1 × 107cfu/
mL；B.50% carbendazol wettable powder；C. clear water compares.
Selection is 14m according to every piece of field area with having infected the planting asparagus of Asparagus Stem Blight2, amount to 9 pieces of fields.By institute
State 9 pieces of fields and be divided into 3 processing, each processing is repeated 3 times, and isolation strip is set between each processing.By the 1 × 10 of 1000mL per acre7cfu/
ML bacillus amyloliquefaciens agent MY001,100 grams of 50% carbendazol wettable powder, carries out 500 times respectively and is watered per acre,
Sprayed respectively in sprout early period, asparagus pumping phase and normal growth phase of asparagus, spray frequency as interval 7~10 days 1 time,
Spray altogether 6~9 times, blank control group spray clear water, whole spraying time is no more than 60 days.Later growth counts everywhere respectively
The disease severity of reason, and anti-efficiency is calculated, it the results are shown in Table 4.
4 field test results of table
| Processing | Degree of disease (%) | Control rate (%) |
| Bacillus amyloliquefaciens MY001 | 28.23 | 69.37 |
| Carbendazim | 22.64 | 74.25 |
| Control group | 90.72 | / |
Table of field test results is bright, and bacillus amyloliquefaciens MY001 can effectively prevent the withered stem disease of asparagus, its effective control rate
It is suitable with the carbendazol wettable powder of chemical agent 50%, embody good prevention effect compared with clear water compares CK.
Sequence table
<110>Chengdu Inst. of Biology, Chinese Academy of Sciences
<120>One bacillus amyloliquefaciens and its application
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1452
<212> DNA
<213>Bacillus amyloliquefaciens (Bacillus amyloliquefaciens)
<400> 1
gagggcgggg tgctatacat gcaagtcgag cggacagatg ggagcttgct ccctgatgtt 60
agcggcggac gggtgagtaa cacgtgggta acctgcctgt aagactggga taactccggg 120
aaaccggggc taataccgga tggttgtctg aaccgcatgg ttcagacata aaaggtggct 180
tcggctacca cttacagatg gacccgcggc gcattagcta gttggtgagg taacggctca 240
ccaaggcgac gatgcgtagc cgacctgaga gggtgatcgg ccacactggg actgagacac 300
ggcccagact cctacgggag gcagcagtag ggaatcttcc gcaatggacg aaagtctgac 360
ggagcaacgc cgcgtgagtg atgaaggttt tcggatcgta aagctctgtt gttagggaag 420
aacaagtgcc gttcaaatag ggcggcacct tgacggtacc taaccagaaa gccacggcta 480
actacgtgcc agcagccgcg gtaatacgta ggtggcaagc gttgtccgga attattgggc 540
gtaaagggct cgcaggcggt ttcttaagtc tgatgtgaaa gcccccggct caaccgggga 600
gggtcattgg aaactgggga acttgagtgc agaagaggag agtggaattc cacgtgtagc 660
ggtgaaatgc gtagagatgt ggaggaacac cagtggcgaa ggcgactctc tggtctgtaa 720
ctgacgctga ggagcgaaag cgtggggagc gaacaggatt agataccctg gtagtccacg 780
ccgtaaacga tgagtgctaa gtgttagggg gtttccgccc cttagtgctg cagctaacgc 840
attaagcact ccgcctgggg agtacggtcg caagactgaa actcaaagga attgacgggg 900
gcccgcacaa gcggtggagc atgtggttta attcgaagca acgcgaagaa ccttaccagg 960
tcttgacatc ctctgacaat cctagagata ggacgtcccc ttcgggggca gagtgacagg 1020
tggtgcatgg ttgtcgtcag ctcgtgtcgt gagatgttgg gttaagtccc gcaacgagcg 1080
caacccttga tcttagttgc cagcattcag ttgggcactc taaggtgact gccggtgaca 1140
aaccggagga aggtggggat gacgtcaaat catcatgccc cttatgacct gggctacaca 1200
cgtgctacaa tggacagaac aaagggcagc gaaaccgcga ggttaagcca atcccacaaa 1260
tctgttctca gttcggatcg cagtctgcaa ctcgactgcg tgaagctgga atcgctagta 1320
atcgcggatc agcatgccgc ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac 1380
accacgagag tttgtaacac ccgaagtcgg tgaggtaacc tttatggagc cagccgccga 1440
agtgacagat gg 1452
<210> 2
<211> 973
<212> DNA
<213>Bacillus amyloliquefaciens (Bacillus amyloliquefaciens)
<400> 2
tcagggtctg tctgcagcgt tttcgttcta ccgggcgctc acggatgtgc gtgacggtct 60
gaagccggtt cacaggcgga ttttgtacgc aatgaatgat ttaggcatga ccagtgacaa 120
accatataaa aaatctgccc gtatcgtcgg tgaagttatc ggtaagtacc acccgcacgg 180
tgactcagcg gtttacgaat caatggtcag aatggcgcag gattttaact accgctacat 240
gcttgttgac ggacacggca acttcggttc ggttgacggc gactcagcgg ccgcgatgcg 300
ttacacagaa gcgagaatgt caaaaatcgc aatggaaatc ctccgggata ttacgaaaga 360
tacgattgat tatcaagata actatgacgg cgcagaaaga gaacctgtcg tcatgccttc 420
gagatttccg aatctgctcg taaacggagc tgccggtatt gcggtcggaa tggcgacaaa 480
tattcctccg catcagcttg gggaagtcat tgaaggcgtg cttgccgtaa gcgagaatcc 540
tgagattaca aaccaggagc tgatggaata catcccgggc ccggattttc cgactgcagg 600
tcagattttg ggccggagcg gcatccgcaa ggcatatgaa tccggacggg gatcaatcac 660
gatccgggct aaggctgaaa tcgaagagac atcatcggga aaagaaagaa ttattgtcac 720
agaacttcct tatcaggtga acaaagcgag attaattgaa aaaatcgcag atcttgtccg 780
ggacaaaaaa atcgaaggaa ttacggatct gcgagacgaa tccgaccgta acggaatgag 840
aatcgtcatt gagatccgcc gtgacgccaa tgctcacgtc attttgaata acctgtacaa 900
acaaacggcc ctgcagacgt ctttcggaat caacctgctg gcgctcgtga cggacagcca 960
aagtcctagc aac 973
<210> 3
<211> 1143
<212> DNA
<213>Bacillus amyloliquefaciens (Bacillus amyloliquefaciens)
<400> 3
ctacgtgtgc acgtcgaaca gtattgacga agccctggcc ggttattgta cagatattaa 60
catcgagatt gaaaaagata acagcattac cgttaaggac aacggacgcg gaattccggt 120
cggtatccag gagaagatgg gccgccctgc ggttgaagtc atcatgaccg ttctccacgc 180
cggcggtaaa tttgacggaa gcggatataa agtatccggc ggtcttcacg gtgtaggggc 240
gtctgtcgta aacgccttgt cgaccactct tgacgttacg gttcatcgtg acggaaaaat 300
ccactatcag gcgtacgagc gcggtgtacc tgtggccgat cttgaagtga tcggtgatac 360
tgataagacc ggaacgatta cgcacttcgt tccggatccg gaaattttca aagaaacaac 420
cgtatacgac tatgacctgc tttcaaaccg tgtccgggaa ttggccttcc tgacaaaagg 480
cgtaaacatc acgattgaag acaaacgtga aggacaagaa cggaaaaacg agtaccacta 540
cgaaggcgga atcaaaagct atgttgagta cttaaaccgt tccaaagaag tcgttcatga 600
agagccgatt tatatcgaag gcgagaaaga cggcataacg gttgaagttg cgttgcaata 660
caacgacagc tatacaagca acatttattc tttcacaaat aacatcaaca catacgaagg 720
cgggacgcac gaagccggat ttaaaaccgg tctgacccgt gtcataaacg actatgcaag 780
aagaaaaggg attttcaaag aaaatgatcc gaatttaagc ggggatgatg tgagagaagg 840
gctgactgcc attatttcaa ttaagcaccc tgatccgcaa ttcgaagggc agacgaaaac 900
gaagctcggc aactccgaag cgagaacgat cactgatacg ctgttttctt ctgcgctgga 960
aacattcctt cttgaaaatc cggactcagc ccgcaaaatc gttgaaaaag gtttaatggc 1020
cgcaagagcg cggatggcag cgaaaaaagc acgggaattg acccggcgca aaagtgcgct 1080
tgagatttcc aatctgccgg gcaaactggc ggactgtctc taaagatccg agcatccggt 1140
caa 1143
Claims (7)
- A 1. bacillus amyloliquefaciens, it is characterised in that：On July 26th, 2017 is preserved in Chinese microorganism strain preservation management Committee's common micro-organisms center, strain name:Bacillus amyloliquefaciens, Bacillus amyloliquefaciens, MY001., deposit number is：CGMCC NO.14460.
- 2. a bacillus amyloliquefaciens, Bacillus amyloliquefaciens, it is characterised in that：Its 16SrDNA is sequenced Sequence is as shown in SEQ ID NO 1, its gyrA sequencing sequence is as shown in SEQ ID NO 2, its gyrB sequencing sequence such as SEQ ID Shown in NO 3.
- 3. application of the bacillus amyloliquefaciens in fungal diseases of plants prevention described in claim 1 or 2.
- 4. application of the bacillus amyloliquefaciens as claimed in claim 3 in fungal diseases of plants prevention, it is characterised in that：Institute Stating fungi includes：Lucid asparagus Phomopsis bacterium, intend Pestalotia bacterium, Curvularia bacterium.
- 5. application of the bacillus amyloliquefaciens as claimed in claim 3 in fungal diseases of plants prevention, it is characterised in that：Institute State application of the bacillus amyloliquefaciens in Asparagus Stem Blight.
- 6. application of the bacillus amyloliquefaciens as claimed in claim 5 in fungal diseases of plants prevention, it is characterised in that：Solution Bacillus amyloliquefaciens are used to prevent the application method of Asparagus Stem Blight：It is 1 × 10 by concentration7The solution starch gemma bar of cfu/mL Bacterium MY001 bacteria suspensions, carry out 500~1000 times dilution, respectively asparagus sprout early period, asparagus pumping phase and normal growth phase into Row sprays, and sprays frequency to be spaced 5~10 days 1 time, sprays altogether 5~10 times, whole spraying time is no more than 60 days.
- 7. application of such as bacillus amyloliquefaciens of claim 3~5 in fungal diseases of plants prevention, it is characterised in that：Institute State using including preventing applied to fungal diseases of plants using the bacillus amyloliquefaciens as active ingredient, also include being metabolized with it Product is prevented for active ingredient applied to fungal diseases of plants.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810029297.2A CN108034611A (en) | 2018-01-12 | 2018-01-12 | One bacillus amyloliquefaciens and its application |
| CN201810434828.6A CN108570432B (en) | 2018-01-12 | 2018-05-09 | Bacillus amyloliquefaciens and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810029297.2A CN108034611A (en) | 2018-01-12 | 2018-01-12 | One bacillus amyloliquefaciens and its application |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108034611A true CN108034611A (en) | 2018-05-15 |
Family
ID=62099292
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810029297.2A Pending CN108034611A (en) | 2018-01-12 | 2018-01-12 | One bacillus amyloliquefaciens and its application |
| CN201810434828.6A Active CN108570432B (en) | 2018-01-12 | 2018-05-09 | Bacillus amyloliquefaciens and application thereof |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810434828.6A Active CN108570432B (en) | 2018-01-12 | 2018-05-09 | Bacillus amyloliquefaciens and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN108034611A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111575210A (en) * | 2020-05-26 | 2020-08-25 | 浙江工业大学 | Bacillus amyloliquefaciens ZJB19161 and application thereof |
| CN113966747A (en) * | 2021-06-30 | 2022-01-25 | 中国热带农业科学院环境与植物保护研究所 | Nano-silver composite bacillus agent for preventing and treating syzygium samarangense fruit rot and anthracnose of syzygium samarangense fruit and preparation method thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109929782B (en) * | 2019-04-12 | 2022-10-11 | 江西省科学院微生物研究所 | Bacillus subtilis for inhibiting phomopsis and application thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20060021162A (en) * | 2004-09-02 | 2006-03-07 | 김진호 | Antifungal plant growth promoting rhizobacterium, bacillus amyloliquefaciens mj-3 and microbial bed soil using hereof |
| CN102154186B (en) * | 2011-04-14 | 2012-09-05 | 中国农业科学院烟草研究所 | Bacillus subtilis and use thereof in prevention and control of fungus disease |
| CN103087951B (en) * | 2013-01-14 | 2014-08-20 | 北京市农林科学院 | Bacillus amyloliquefaciens and application thereof |
| CN103087960A (en) * | 2013-01-29 | 2013-05-08 | 江苏省农业科学院 | Bacillus amyloliquefaciens FQS38 and application thereof |
| CN103642727B (en) * | 2013-12-02 | 2016-03-30 | 北京市农林科学院 | The bacillus amyloliquefaciens of one strain Antifungi and application thereof |
| CN106883996B (en) * | 2015-12-16 | 2020-04-28 | 中国食品发酵工业研究院有限公司 | Biocontrol strain LS01 for preventing and treating stem blight of asparagus and biocontrol microbial inoculum thereof |
-
2018
- 2018-01-12 CN CN201810029297.2A patent/CN108034611A/en active Pending
- 2018-05-09 CN CN201810434828.6A patent/CN108570432B/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111575210A (en) * | 2020-05-26 | 2020-08-25 | 浙江工业大学 | Bacillus amyloliquefaciens ZJB19161 and application thereof |
| CN113966747A (en) * | 2021-06-30 | 2022-01-25 | 中国热带农业科学院环境与植物保护研究所 | Nano-silver composite bacillus agent for preventing and treating syzygium samarangense fruit rot and anthracnose of syzygium samarangense fruit and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108570432B (en) | 2021-01-01 |
| CN108570432A (en) | 2018-09-25 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111500501B (en) | Streptomyces misonii strain and application thereof in preventing and treating wheat root rot and stem basal rot | |
| CN110205273B (en) | Bacillus amyloliquefaciens with growth promoting and disease resisting effects and application thereof | |
| CN110172428B (en) | Bacillus subtilis with disease resistance and fruit quality improvement functions and application thereof | |
| CN113717901B (en) | Bacillus bailii and application thereof in preventing and controlling various vegetable diseases | |
| CN105441366B (en) | Methylotrophic bacillus ZBL-1 is applied in preventing cotton verticillium wilt | |
| CN113755381A (en) | Bacillus licheniformis for preventing and treating plant diseases and application thereof | |
| CN112359000B (en) | Efficient biocontrol pseudomonas and application thereof in prevention and control of rice diseases | |
| CN106591157A (en) | Aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and application of aspergillus tubingensis metabolites | |
| CN110982725B (en) | Bacillus for antagonizing fusarium wilt and promoting growth and application thereof | |
| CN108034611A (en) | One bacillus amyloliquefaciens and its application | |
| CN105349455B (en) | One plant of Malaysian streptomycete and its application | |
| CN112795496A (en) | Paenibacillus polymyxa and application thereof in preventing and treating stem basal rot of Chinese cabbage | |
| CN107075459A (en) | Novel bacterial of bacillus and application thereof | |
| CN110982724A (en) | Bacillus for antagonizing phytopathogen and promoting rooting and application thereof | |
| CN110964654A (en) | Bacillus antagonistic to fusarium wilt and application thereof | |
| CN104726384A (en) | Bacillus subtilis for prohibiting rhizoctonia solani of potatoes | |
| CN114806928A (en) | Pepper endophytic Bacillus beleisi PEB23 and application thereof | |
| CN114276957A (en) | Biocontrol strain with effect of preventing and treating various plant pathogenic fungi and application thereof | |
| CN113801807A (en) | Growth-promoting microbial inoculum for inducing systemic resistance of processed tomatoes and application thereof | |
| CN108998395B (en) | Bacillus amyloliquefaciens and application thereof | |
| CN116445354A (en) | Pseudomonas friedrisburgh and application thereof in disease prevention | |
| CN108587969B (en) | Preparation and application of verticillium dahliae strain HCX-01 capable of preventing and treating cotton verticillium wilt | |
| CN113817642B (en) | Bacillus bailii YJ02, microbial preparation and application thereof | |
| CN111363691A (en) | Paenibacillus polymyxa and application thereof | |
| CN109504640A (en) | Bacillus subtilis GU MT323 and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180515 |
|
| WD01 | Invention patent application deemed withdrawn after publication |