CN108030766A - Placenta Subaerial blue green algae gel preparation with long preservation period and preparation method - Google Patents
Placenta Subaerial blue green algae gel preparation with long preservation period and preparation method Download PDFInfo
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- CN108030766A CN108030766A CN201711386091.7A CN201711386091A CN108030766A CN 108030766 A CN108030766 A CN 108030766A CN 201711386091 A CN201711386091 A CN 201711386091A CN 108030766 A CN108030766 A CN 108030766A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/06—Ointments; Bases therefor; Other semi-solid forms, e.g. creams, sticks, gels
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/48—Reproductive organs
- A61K35/50—Placenta; Placental stem cells; Amniotic fluid; Amnion; Amniotic stem cells
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/24—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite containing atoms other than carbon, hydrogen, oxygen, halogen, nitrogen or sulfur, e.g. cyclomethicone or phospholipids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
Abstract
The present invention provides a kind of placenta Subaerial blue green algae gel preparation with long preservation period, which is made of placenta Subaerial blue green algae and gel-type vehicle, and 0.5 × 10 is contained in gel-type vehicle described in per g6‑2×106A placenta Subaerial blue green algae, the gel-type vehicle is mainly made of deionized water, stearic acid sodium lactate, sodium alginate micro-capsule and glycerine, this adds sodium alginate micro-capsule in gel-type vehicle can improve the stability of gel preparation, it can be used for preserving placenta Subaerial blue green algae for a long time so that placenta Subaerial blue green algae has very high survival rate.
Description
Technical field
The invention belongs to gel preparation technical field, more particularly to a kind of placenta Subaerial blue green algae with long preservation period coagulates
Glue preparation and preparation method.
Background technology
Placenta Subaerial blue green algae is derived from family's Subaerial blue green algae of neonate's placenta tissue, it is in the stage of development
Approached with embryonic stem cell, but also with many biological properties of multipotential stem cell, using distribution type is not required to, quantity is enriched, differentiation
Ability is strong, and orientable to be divided into mescenchymal stem cell, vascular stem cell, epithelial stem cell, neural stem cell, liver stem cells etc. more
Kind stem cell, it is with anti-aging, the figure that gets well, diseases of cardiovascular and cerebrovascular systems, diabetes, lesions of liver and kidney, brain and spinal cord
Neurotrosis, autoimmune disease, graft versus host disease(GVH disease) and candidate stem cell co-transplantation treatment blood disease, ischemic blood
Pipe disease, lung and other histoorgan fibrosis.
Gel preparation is a kind of novel pharmaceutical formulation of domestic rising in recent years, means that medicine is made uniformly with suitable auxiliary material
Or the transparent or semitransparent semisolid preparation being suspended.Preparing the common matrix of gel includes hypromellose, acrylic acid
Resin etc., gel preparation can use substantial amounts of medical high-molecular additive in preparation process, it seriously affects the matter of gel preparation
Amount and each performance.
The content of the invention
In order to solve the above technical problem, the present invention provides a kind of placenta Subaerial blue green algae gel system with long preservation period
Agent, the jelly gel preparation have good stability and other every performances.
Concrete technical scheme of the present invention is as follows：
The present invention provides a kind of placenta Subaerial blue green algae gel preparation with long preservation period, and the gel preparation is sub- by placenta
Myeloid-lymphoid stem cell and gel-type vehicle form, and 0.5 × 10 is contained in gel-type vehicle described in per g6-2×106A placenta Asia all can be done carefully
Born of the same parents, the gel-type vehicle are mainly made of the component of following parts by weight：
Further to improve, the sodium alginate micro-capsule is prepared by following parts by weight component：
Sodium alginate 4-6
Sodium Glycinate 0.7-1.4
Sodium starch glycol 0.3-0.6.
By adding sodium alginate micro-capsule one side in gel preparation, it can play the role of gel-type vehicle to the present invention,
On the other hand the usage amount of gel-type vehicle can be reduced, and hypromellose, third are not used in whole gel preparation
Gel preparation can be made in the medical high-molecular additives such as olefin(e) acid resin, and the stability of the gel preparation is good, available for growing
Time preserves placenta Subaerial blue green algae so that the placenta Subaerial blue green algae of preservation has very high survival rate.
Further to improve, which is arriving of being prepared via a method which：By Sodium Glycinate and 2/3 weight
The sodium alginate of amount part, which is placed in 75 DEG C of water-baths, to be heated, and adds the sodium starch glycol of 1/3 parts by weight, rotating speed 5000 while hot
Turn/min, stir 5min, add the deionized water of 1.05 times of parts by weight of Sodium Glycinate, rotating speed is 1000 turns/min, stirring
15min, adds the sodium alginate of 1/3 parts by weight, and rotating speed is 4200 turns/min, stirs 6min, mixture is made, into mixture
The sodium starch glycol of 1/3 parts by weight is added, continues to stir, rotating speed is 6000 turns/min, stirs 8min, obtains uniform thing, to
Cold petroleum ether is added in uniform thing, continues to stir, solidifies uniform thing, is filtered, is spontaneously dried, it is 25- that particle size range, which is made,
50 μm of sodium alginate micro-capsule.
The preparation process of stringent control sodium alginate micro-capsule, can improve the ratio of briquetting of sodium alginate micro-capsule, improve seaweed
The dispersion rate of sour sodium micro-capsule in water, and then the solubility property of the sodium alginate in water is improved, ensure that it can be played very well
Ground keeps the performance of placenta Subaerial blue green algae motility rate.
Further to improve, it is compound that the gel-type vehicle further includes the phosphatidyl-ethanolamine that parts by weight are 1.8-3.8 parts
Thing.
Further to improve, the phosphatidyl ethanol amine compound is prepared by the component of following parts by weight：
Phosphatidyl-ethanolamine 1.2-2.5
Maltitol 0.4-0.8
Sodium chloride 0.2-0.5.
For the present invention by adding phosphatidyl ethanol amine compound in gel-type vehicle, it can significantly improve gel preparation
Transdermal absorption performance, can also improve dispersiveness of the placenta Subaerial blue green algae in gel-type vehicle, and the phosphatidyl added
Ethanol amine compound will not produce interference and suction-operated to placenta Subaerial blue green algae, not interfere with placenta Subaerial blue green algae
Activity, its can be formed around placenta Subaerial blue green algae one folder medicine film so that placenta Subaerial blue green algae can
The effect of sustained release is played, Quadratic Stability can also be played the role of.
Further to improve, the gel-type vehicle further includes the octadecyldimethyl that parts by weight are 0.06-0.12 parts
Glycine betaine and 0.1-0.3 parts of meglumine.
The present invention can significantly improve gel by adding octodecyl betaine and meglumine in gel-type vehicle
The caking property of preparation and skin, can significantly reduce irritation of the gel preparation to skin.
Further to improve, the gel preparation is mainly made of the component of following parts by weight：
Deionized water 87
Stearic acid sodium lactate 1.5
Sodium alginate micro-capsule
Phosphatidyl ethanol amine compound
Another aspect of the present invention provides a kind of preparation method of placenta Subaerial blue green algae gel preparation with long preservation period,
This method comprises the following steps：
S1：Prepare sodium alginate micro-capsule；
S2：Prepare phosphatidyl ethanol amine compound；
S3：Sodium alginate micro-capsule is dissolved in deionized water, stood liquid, adds glycerine and stearic acid sodium lactate, stirring
10min, 3500 turns/min of rotating speed, add phosphatidyl ethanol amine compound, octodecyl betaine and meglumine, stirring
10min, 1200 turns/min of rotating speed, are made gel-type vehicle, to contain 0.5 × 10 in gel-type vehicle described in every g6-2×106A placenta
The amount of Subaerial blue green algae adds placenta Subaerial blue green algae, stirs evenly to obtain the final product.
Further to improve, the preparation method of the sodium alginate micro-capsule is：The preparation method of the sodium alginate micro-capsule
For：The sodium alginate of Sodium Glycinate and 2/3 parts by weight is placed in 75 DEG C of water-baths and is heated, adds the starch of 1/3 parts by weight while hot
Sodium glycolate, rotating speed are 5000 turns/min, stir 5min, add the deionized water of 1.05 times of parts by weight of Sodium Glycinate, and rotating speed is
1000 turns/min, 15min is stirred, adds the sodium alginate of 1/3 parts by weight, rotating speed is 4200 turns/min, stirs 6min, is made mixed
Compound, the sodium starch glycol of 2/3 parts by weight is added into mixture, continues to stir, and rotating speed is 6000 turns/min, stirring
8min, obtains uniform thing, and cold petroleum ether is added into uniform thing, continues to stir, and solidifies uniform thing, filters, and spontaneously dries,
The sodium alginate micro-capsule that particle size range is 25-50 μm is made.
Further to improve, the preparation method of the phosphatidyl ethanol amine compound is：By phosphatidyl-ethanolamine and malt
Sugar alcohol is dissolved in ethanol, and the weight part ratio of phosphatidyl-ethanolamine, the total weight parts of maltitol and ethanol is 1:1.1, in rotation
Film forming is evaporated under reduced pressure on evaporimeter, sodium chloride is soluble in water, and the weight part ratio of sodium chloride and water is 1:0.9, aqueous solution is made,
By into film be dissolved in aqueous solution, after aquation, filtering, is made phosphatidyl ethanol amine compound.
Gel preparation prepared by the present invention has very high stability.
Placenta Subaerial blue green algae gel preparation with long preservation period provided by the invention, adds seaweed in gel-type vehicle
Sour sodium micro-capsule can improve the stability of gel preparation, can be used for preserving placenta Subaerial blue green algae for a long time so that placenta
Subaerial blue green algae has very high survival rate.
Embodiment
A kind of 1 gel preparation of embodiment
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, contains 0.5 in gel-type vehicle described in per g
×106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 82g
Stearic acid sodium lactate 1.1g
Sodium alginate micro-capsule
A kind of 2 gel preparation of embodiment
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, contains 1.5 in gel-type vehicle described in per g
×106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 87g
Stearic acid sodium lactate 1.7g
Sodium alginate micro-capsule
Sodium alginate micro-capsule is prepared via a method which to obtain:The sodium alginate of 1.2g Sodium Glycinates and 3.8g is put
Heated in 75 DEG C of water-baths, add the sodium starch glycol of 0.1g while hot, rotating speed is 5000 turns/min, stirs 5min, is added sweet
The deionized water of propylhomoserin sodium 1.26g, rotating speed are 1000 turns/min, stir 15min, add the sodium alginate of 1.9g, and rotating speed is
4200 turns/min, 6min is stirred, mixture is made, the sodium starch glycol of 0.2g is added into mixture, continues to stir, rotating speed
For 6000 turns/min, 8min is stirred, uniform thing is obtained, cold petroleum ether is added into uniform thing, continue to stir, coagulate uniform thing
Gu filtration, spontaneously dries, the sodium alginate micro-capsule that particle size range is 25 μm is made.
A kind of 3 gel preparation of embodiment
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 86g
Stearic acid sodium lactate 1.4g
Sodium alginate micro-capsule
The preparation method of the gel preparation is：
S1：Prepare sodium alginate micro-capsule：With embodiment 2；
S2：Sodium alginate micro-capsule is dissolved in 86g deionized waters, stood liquid, adds 1.8g glycerine and 1.4g stearic acid
Sodium lactate, stirs 5min, 2000 turns/min of rotating speed, and gel-type vehicle is made, to contain 1 × 10 in gel-type vehicle described in every g6A tire
The amount of disk Subaerial blue green algae adds placenta Subaerial blue green algae, stirs evenly to obtain the final product.
A kind of 4 gel preparation of embodiment
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 85g
Stearic acid sodium lactate 1.5g
Sodium alginate micro-capsule
The preparation method of the gel preparation is the same as embodiment 3.
A kind of 5 gel preparation of embodiment
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 85g
Stearic acid sodium lactate 1.6g
Sodium alginate micro-capsule
Phosphatidyl ethanol amine compound
Phosphatidyl-ethanolamine 1.5g
Maltitol 0.6g
Sodium chloride 0.4g
Sodium alginate micro-capsule is prepared via a method which to obtain:The sodium alginate of 1.3g Sodium Glycinates and 3g is placed in
Heated in 75 DEG C of water-baths, add the sodium starch glycol of 0.15g while hot, rotating speed is 5000 turns/min, stirs 5min, adds sweet ammonia
The deionized water of sour sodium 1.365g, rotating speed are 1000 turns/min, stir 15min, add the sodium alginate of 1.5g, rotating speed 4200
Turn/min, stir 6min, mixture is made, the sodium starch glycol of 0.3g is added into mixture, continues to stir, rotating speed is
6000 turns/min, 8min is stirred, uniform thing is obtained, cold petroleum ether is added into uniform thing, continue to stir, coagulate uniform thing
Gu filtration, spontaneously dries, the sodium alginate micro-capsule that particle size range is 50 μm is made；
Phosphatidyl ethanol amine compound is prepared via a method which to obtain：By 1.5g phosphatidyl-ethanolamines and 0.6g wheats
Bud sugar alcohol is dissolved in 2.31g ethanol, and in film forming is evaporated under reduced pressure on Rotary Evaporators, 0.4g sodium chloride is dissolved in 0.36g water, will
Into film be dissolved in aqueous solution, after aquation, filtering, is made phosphatidyl ethanol amine compound.
A kind of 6 gel preparation of embodiment
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 84g
Stearic acid sodium lactate 1.5g
Sodium alginate micro-capsule
Phosphatidyl ethanol amine compound
Phosphatidyl-ethanolamine 1.7g
Maltitol 0.7g
Sodium chloride 0.3g
The preparation method of the gel preparation is：
S1：Prepare sodium alginate micro-capsule：With embodiment 5；
S2：Prepare phosphatidyl ethanol amine compound：With embodiment 5；
S3：Sodium alginate micro-capsule is dissolved in 84g deionized waters, stood liquid, adds 2.2g glycerine and 1.5g stearic acid
Sodium lactate, stirring 5min, 2000 turns/min of rotating speed, addition phosphatidyl ethanol amine compound, stirring 10min, 1200 turns of rotating speed/
Min, is made gel-type vehicle, to contain 1 × 10 in gel-type vehicle described in every g6The amount of a placenta Subaerial blue green algae adds placenta
Subaerial blue green algae, stirs evenly to obtain the final product.
A kind of 7 gel preparation of embodiment
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 85g
Stearic acid sodium lactate 1.5g
Sodium alginate micro-capsule
Phosphatidyl ethanol amine compound
Phosphatidyl-ethanolamine 2.2g
Maltitol 0.5g
Sodium chloride 0.4g
The preparation method of the gel preparation is the same as embodiment 6.
A kind of 8 gel preparation of embodiment
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 87g
Stearic acid sodium lactate 1.5g
Sodium alginate micro-capsule
Phosphatidyl ethanol amine compound
The preparation method of the gel preparation is：
S1：Prepare sodium alginate micro-capsule：With embodiment 5；
S2：Prepare phosphatidyl ethanol amine compound：With embodiment 5；
S3：Sodium alginate micro-capsule is dissolved in 87g deionized waters, stood liquid, adds 2g glycerine and 1.5g tristearin yogurts
Sour sodium, stirs 5min, 2000 turns/min of rotating speed, adds phosphatidyl ethanol amine compound, octodecyl betaine and Portugal
Methylamine, stirs 10min, 1200 turns/min of rotating speed, and gel-type vehicle is made, to contain 1 × 10 in gel-type vehicle described in every g6A tire
The amount of disk Subaerial blue green algae adds placenta Subaerial blue green algae, stirs evenly to obtain the final product.
A kind of 9 gel preparation of embodiment
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 85g
Stearic acid sodium lactate 1.3g
Sodium alginate micro-capsule
Phosphatidyl ethanol amine compound
The preparation method of the gel preparation is the same as embodiment 8.
A kind of 10 gel preparation of embodiment
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 90g
Stearic acid sodium lactate 1.8g
Sodium alginate micro-capsule
Phosphatidyl ethanol amine compound
The preparation method of the gel preparation is the same as embodiment 8.
A kind of 1 gel preparation of reference examples
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, contains 1.5 in gel-type vehicle described in per g
×106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 87g
Stearic acid sodium lactate 1.7g
Sodium alginate micro-capsule
Sodium alginate micro-capsule is prepared via a method which to obtain:By the sodium alginate of 1.2g Sodium Glycinates and 5.7g and
0.3g sodium starch glycols are placed in 75 DEG C of water-baths and heat, and rotating speed is 5000 turns/min, stir 20min, obtain uniform thing, Xiang Jun
Cold petroleum ether is added in even thing, continues to stir, solidifies uniform thing, is filtered, is spontaneously dried, it is 25 μm that particle size range, which is made,
Sodium alginate micro-capsule.
A kind of 2 gel preparation of reference examples
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, contains 1.5 in gel-type vehicle described in per g
×106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
It is prepared by conventional method.
A kind of 3 gel preparation of reference examples
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 86g
Odium stearate 1.4g
Acrylic resin micro-capsule
Sodium alginate is cached acrylic resin, Sodium Glycinate is cached by the preparation method of the gel preparation with embodiment 3
Sodium glutamate.
A kind of 4 gel preparation of reference examples
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 86g
Stearic acid sodium lactate 1.4g
Sodium alginate micro-capsule
The preparation method preparation method of the gel preparation changes sodium starch glycol into dodecyl sulphate with embodiment 3
Sodium.
A kind of 5 gel preparation of reference examples
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 85g
Stearic acid sodium lactate 1.6g
Sodium alginate micro-capsule
Phosphatidylserine complex
Phosphatidylserine 1.5g
Maltitol 0.6g
Sodium alginate micro-capsule is prepared via a method which to obtain:The sodium alginate of 1.3g Sodium Glycinates and 3g is placed in
Heated in 75 DEG C of water-baths, add the sodium starch glycol of 0.15g while hot, rotating speed is 5000 turns/min, stirs 5min, adds sweet ammonia
The deionized water of sour sodium 1.365g, rotating speed are 1000 turns/min, stir 15min, add the sodium alginate of 1.5g, rotating speed 4200
Turn/min, stir 6min, mixture is made, the sodium starch glycol of 0.3g is added into mixture, continues to stir, rotating speed is
6000 turns/min, 8min is stirred, uniform thing is obtained, cold petroleum ether is added into uniform thing, continue to stir, coagulate uniform thing
Gu filtration, spontaneously dries, the sodium alginate micro-capsule that particle size range is 50 μm is made；
Phosphatidylserine complex is prepared via a method which to obtain：By 1.5g phosphatidylserines and 0.6g wheats
Bud sugar alcohol is dissolved in 2.31g ethanol, on Rotary Evaporators be evaporated under reduced pressure film forming, by into film it is soluble in water, after aquation, mistake
Filter, is made serine compound.
A kind of 6 gel preparation of reference examples
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 84g
Stearic acid sodium lactate 1.5g
Sodium alginate micro-capsule
The preparation method of the gel preparation is：
S1：Prepare sodium alginate micro-capsule：With embodiment 5；
S2：Sodium alginate micro-capsule is dissolved in 84g deionized waters, stood liquid, adds 2.2g glycerine and 1.5g stearic acid
Sodium lactate, stirs 5min, 2000 turns/min of rotating speed, adds 1.7g phosphatidyl-ethanolamines, 0.7g maltitols and 0.3g sodium chloride,
10min, 1200 turns/min of rotating speed are stirred, gel-type vehicle is made, to contain 1 × 10 in gel-type vehicle described in every g6A placenta is sub- complete
The amount of energy stem cell adds placenta Subaerial blue green algae, stirs evenly to obtain the final product.
A kind of 7 gel preparation of reference examples
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 84g
Stearic acid sodium lactate 1.5g
Sodium alginate micro-capsule
Phosphatidyl ethanol amine compound
Phosphatidyl-ethanolamine 1.7g
Mannitol 0.7g
Sodium chloride 0.3g
The preparation method of the gel preparation is：With embodiment 6, maltitol is changed into mannitol.
A kind of 8 gel preparation of reference examples
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 87g
Stearic acid sodium lactate 1.5g
Sodium alginate micro-capsule
Phosphatidyl ethanol amine compound
The preparation method of the gel preparation is：Octodecyl betaine is changed into dodecyl sulphur with embodiment 8
Sour sodium.
A kind of 9 gel preparation of reference examples
The gel preparation is made of placenta Subaerial blue green algae and gel-type vehicle, per gel-type vehicle described in g in containing 1 ×
106A placenta Subaerial blue green algae, the gel-type vehicle are mainly made of the component of following weight：
Deionized water 87g
Stearic acid sodium lactate 1.5g
Sodium alginate micro-capsule
Phosphatidyl ethanol amine compound
The preparation method of the gel preparation is：Meglumine is changed into glucan with embodiment 8.
1 gel preparation of experimental example freezes the detection of rear placenta Subaerial blue green algae vigor
Take the gel preparation of 2-4 of the embodiment of the present invention and reference examples 1-4, be placed in liquid nitrogen, respectively freeze 1 month, 2
Take out within a month, 3 months, 6 months, 9 months and 12 months, be placed in 37 DEG C of water-bath, recovery 1-2min, is expected with platform from liquid nitrogen
Blue decoration method carries out cell count, calculates placenta Subaerial blue green algae survival rate, and testing result is shown in Table 1.
The testing result of 1 each embodiment of table and reference examples gel preparation placenta Subaerial blue green algae motility rate
As can be seen from the table, gel preparation property provided by the invention is stablized, and it is all-round can to preserve placenta Asia for a long time
Stem cell, after preserving 12 months, cell survival rate shows that gel preparation provided by the invention can be used for growing more than 90.8%
Time preserves mescenchymal stem cell, and the cell survival rate preserved from the gel preparation of reference examples can be seen that when to gel preparation
In each component be replaced, delete or change micro-capsule preparation method can influence cell viability that gel preparation freezes and
The time frozen.
2 vitro skin absorption test of experimental example
Experimental method：Adult male rats are taken, puts to death and peels off skin of abdomen, carrying out depilation processing, be placed in physiological saline
In it is spare, using Franz diffusion cells, effective infiltrating area is 1cm2, reception medium is physiological saline, and acceptance pool volume is 5mL.
Rat skin after processing is uniformly divided into 5 pieces of homalographic, is divided into 1 group of experiment, 2 groups of experiment, control 1-3 groups,
The gel preparation of 1 group of the skin chunk coating embodiment of the present invention 4 is tested, the skin chunk coating embodiment of the present invention 6 that 2 groups of test example
Gel preparation, the gel preparation of reference examples 5-7 is respectively coated in the skin chunk of control 1-3 groups；Every piece of skin coats 0.5g and coagulates
Glue preparation, the skin chunk for coating gel preparation is placed in supply pool and carries out penetrating absorption in 37 DEG C, 500r/min, respectively at
0.5th, 1,2,4,6,8,10,12h removes whole acceptable solutions (while supplementing normal saline), 8000r/min centrifugations
10min, takes supernatant, and the number of placenta Subaerial blue green algae, unit of account area accumulation warp are calculated using trypan blue staining
Skin infiltration capacity Qn, with QnMap to time t, and to the straight line portion linear regression in curve, gained straight slope, that is, steady-state permeation
Speed Js (a/cm2H), 2 be the results are shown in Table.
2 Transdermal Absorption result of the test (n=3) of table
As can be seen from the table, phosphatidyl ethanol amine compound is added in gel-type vehicle and is remarkably improved gel preparation
Percutaneous permeability.
3 vitro skin irritant experiment of experimental example
Adult healthy rabbit 8 is taken, half male and half female, weight 1.5kg, family's rabbit back backbone diamond wool is taken off, face of losing hair or feathers
Product is about 8cm per side2.4 groups are randomly divided into, 2 groups of complete 1 group of experiment, 2 groups of experiment, 1 group of control and control, test 1 group of rabbit and apply
The gel preparation of embodiment 4 is covered, tests the gel preparation of 2 groups of rabbit coating embodiments 8, compares the gel of 1 group of coating reference examples 8
Preparation, compares the gel preparation of 2 groups of coating reference examples 9, continuous coating 7 days, whether there is at 1,24,48, the observation coating of 72h results
The symptom such as erythema and oedema, scores according to table 3, the results are shown in Table 4.
3 skin wound repair standards of grading of table
4 gel preparation irritation appraisal result of table
Show that the present invention adds octodecyl betaine and the mixture of meglumine in gel-type vehicle and can drop
Irritation of the low gel preparation to skin.
Claims (10)
1. a kind of placenta Subaerial blue green algae gel preparation with long preservation period, it is characterised in that the gel preparation is by placenta
Subaerial blue green algae and gel-type vehicle form, and 0.5 × 10 is contained in gel-type vehicle described in per g6-2×106A placenta Asia all can be done
Cell, the gel-type vehicle are mainly made of the component of following parts by weight：
2. placenta Subaerial blue green algae gel preparation with long preservation period as claimed in claim 1, it is characterised in that the sea
Mosanom micro-capsule is prepared by following parts by weight component：
Sodium alginate 4-6
Sodium Glycinate 0.7-1.4
Sodium starch glycol 0.3-0.6.
3. placenta Subaerial blue green algae gel preparation with long preservation period as claimed in claim 2, it is characterised in that the sea
Mosanom micro-capsule is arriving of being prepared via a method which：The sodium alginate of Sodium Glycinate and 2/3 parts by weight is placed in 75 DEG C of water
Heated in bath, add the sodium starch glycol of 1/3 parts by weight while hot, rotating speed is 5000 turns/min, stirs 5min, adds glycine
The deionized water of 1.05 times of parts by weight of sodium, rotating speed are 1000 turns/min, stir 15min, add the sodium alginate of 1/3 parts by weight,
Rotating speed is 4200 turns/min, stirs 6min, and mixture is made, and the sodium starch glycol of 1/3 parts by weight is added into mixture, after
Continuous stirring, rotating speed is 6000 turns/min, stirs 8min, obtains uniform thing, cold petroleum ether is added into uniform thing, continue to stir
Mix, solidify uniform thing, filter, spontaneously dry, the sodium alginate micro-capsule that particle size range is 25-50 μm is made.
4. placenta Subaerial blue green algae gel preparation with long preservation period as claimed in claim 1, it is characterised in that described solidifying
Gel matrix further includes the phosphatidyl ethanol amine compound that parts by weight are 1.8-3.8 parts.
5. placenta Subaerial blue green algae gel preparation with long preservation period as claimed in claim 4, it is characterised in that the phosphorus
Acyl ethanol amine compound is prepared by the component of following parts by weight：
Phosphatidyl-ethanolamine 1.2-2.5
Maltitol 0.4-0.8
Sodium chloride 0.2-0.5.
6. placenta Subaerial blue green algae gel preparation with long preservation period as claimed in claim 5, it is characterised in that described solidifying
Gel matrix further includes the octodecyl betaine and 0.1-0.3 parts of meglumine that parts by weight are 0.06-0.12 parts.
7. placenta Subaerial blue green algae gel preparation with long preservation period as claimed in claim 6, it is characterised in that described solidifying
Gel matrix is mainly made of the component of following parts by weight：
Deionized water 87
Stearic acid sodium lactate 1.5
Sodium alginate micro-capsule
Phosphatidyl ethanol amine compound
8. a kind of preparation method of the placenta Subaerial blue green algae gel preparation with long preservation period described in claim 7, it is special
Sign is that described method includes following steps：
S1：Prepare sodium alginate micro-capsule；
S2：Prepare phosphatidyl ethanol amine compound；
S3：Sodium alginate micro-capsule is dissolved in deionized water, stood liquid, adds glycerine and stearic acid sodium lactate, stirs 5min,
2000 turns/min of rotating speed, adds phosphatidyl ethanol amine compound, octodecyl betaine and meglumine, stirs 10min,
1200 turns/min of rotating speed, is made gel-type vehicle, to contain 0.5 × 10 in gel-type vehicle described in every g6-2×106A placenta Asia is all-round
The amount of stem cell adds placenta Subaerial blue green algae, stirs evenly to obtain the final product.
9. preparation method as claimed in claim 8, it is characterised in that the preparation method of the sodium alginate micro-capsule is：Will be sweet
Propylhomoserin sodium and the sodium alginate of 2/3 parts by weight are placed in 75 DEG C of water-baths and heat, and add the starch glycolate acid of 1/3 parts by weight while hot
Sodium, rotating speed are 5000 turns/min, stir 5min, add 1.05 times of parts by weight of Sodium Glycinate deionized water, rotating speed for 1000 turns/
Min, stirs 15min, adds the sodium alginate of 1/3 parts by weight, and rotating speed is 4200 turns/min, stirs 6min, and mixture is made, to
The sodium starch glycol of 2/3 parts by weight is added in mixture, continues to stir, rotating speed is 6000 turns/min, stirs 8min, is obtained equal
Even thing, cold petroleum ether is added into uniform thing, continues to stir, and solidifies uniform thing, is filtered, and is spontaneously dried, and particle diameter model is made
Enclose the sodium alginate micro-capsule for 25-50 μm.
10. preparation method as claimed in claim 8, it is characterised in that the preparation method of the phosphatidyl ethanol amine compound
For：Phosphatidyl-ethanolamine and maltitol are dissolved in ethanol, phosphatidyl-ethanolamine, the total weight parts of maltitol and ethanol
Weight part ratio is 1:1.1, form a film in being evaporated under reduced pressure on Rotary Evaporators, sodium chloride is soluble in water, the weight of sodium chloride and water
Part is than being 1:0.9, aqueous solution is made, by into film be dissolved in aqueous solution, after aquation, filtering, obtained phosphatidyl-ethanolamine answers
Compound.
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