CN108025019A

CN108025019A - Blood glucose value raises inhibitor and the oral composition containing it

Info

Publication number: CN108025019A
Application number: CN201680053826.1A
Authority: CN
Inventors: 定清刚; 石田有希; 渡边光
Original assignee: Hayashibara Biochemical Laboratories Co Ltd
Current assignee: Hayashibara Co Ltd; Hayashibara Seibutsu Kagaku Kenkyujo KK
Priority date: 2015-09-18
Filing date: 2016-09-15
Publication date: 2018-05-11
Also published as: KR20180053361A; WO2017047706A1; JP7217089B2; CN115475177A; JPWO2017047706A1

Abstract

The present invention is intended to provide the people of the people containing health and care about blood glucose value can be daily easy and safely, and the blood glucose value rise inhibitor after the monosaccharide intake constantly absorbed and the oral composition containing it.The present invention is by providing (A) using glucose as being formed sugar, (B) have through α 1, key connection beyond 4 keys is to positioned at through α 1, the branched structure of the glucose polymerization degree more than 1 of the non-reducing end glucose residue of one end of the straight-chain glucan of the glucose polymerization degree more than 3 of 4 key connections, the blood glucose value that (C) contains the branch's alpha-glucan mixture for digesting generation isomaltose by isomaltose glucanase raise inhibitor and together raise the oral composition of inhibitor containing above-mentioned blood glucose value with monosaccharide and solve.

Description

Blood glucose value raises inhibitor and the oral composition containing it

【Technical field】

The present invention relates to blood glucose value to raise inhibitor, in particular to the liter for suppressing the blood glucose value after monosaccharide absorbs High blood glucose value rise inhibitor and, containing its oral composition.

【Background technology】

In advanced state, the increase of diabetes becomes big social concern, its prevention becomes big problem.In order to prevent or Improve diabetes, recommend the aerobic exercise of control, walking or the running of the amount of having dinner etc., but these are grown under suitable guidance Period, daily carry out being difficult.

Generally, it is believed that the state for maintaining blood glucose value high easily becomes diabetes, particularly, suppress just feeding Blood glucose value afterwards, the rise of so-called postprandial plasma glucose level are important for diabetes mellitus prevention.But in recent years, make as monosaccharide Isomerized sugar obtained from a kind of glucose isomerization (mixture of glucose and fructose) becomes as sweetening material in various foods Widely use in thing, especially taken the photograph compared with cold drink etc. in large quantities in use, although being unaware that through the intake of beverage The chance of monosaccharide is taken to become more.The monosaccharide of glucose or fructose etc. is since intake is absorbed with being easy for and causes blood glucose value Raise, the blood glucose value rise after food of the suppression intake containing these monosaccharides is also important from the viewpoint of prevention diabetes.

Under such situation, the elevated method as the blood glucose value after easily controlling monosaccharide to absorb, it is proposed that utilize The method that the elevated material for then suppressing blood glucose value is together absorbed with monosaccharide.For example, Patent Document 1 discloses from big Blood glucose value rise of the wheat germ breast when obtained β -1,3-1,4- glucans suppress glucose load.But such as in non-patent text Reported in offering 1, β -1,3-1,4- glucans are in impaired glucose tolerance, are had only on the difficult people reduced of blood glucose value, so-called " diabetes side Blood glucose value when glucose load is played in the case of the people in battery limit (BL) domain " raises the limitation of inhibition.In addition, in patent document Disclosed in 2, rat together absorbs the D-Psicose of high-purity with glucose, then blood glucose value rise is suppressed.But D- Ah Lip river ketose is since itself is pleasantly sweet, not only can not be accessible to flavor influence when being applied in combination with pleasantly sweet monose Ground utilizes, and is also unsuitable for it is expected the cooperation such as the tasteless tea beverage for taking agent or sugar-free, approximate water, as there is blood glucose value rise The dry beverage of inhibition, in the embodiment that can not be applied to utilize before and after the food containing monosaccharide is absorbed Point, utilization scope has boundary.

On the one hand, indigestible dextrins, add a small amount of inorganic acid after, process obtained from the roasting dextrin of heating A kind of indigestible dextrins (non-patent literature 2) as water-soluble dietary fiber have suppression due to itself being low sweet taste The rise of postprandial plasma glucose level, makes the stable effect of the secretion of insulin, coordinates with more food, utilizes, but the indigestible Dextrin such as reports that inhibition is not shown in the rise of the blood glucose value after being absorbed for monosaccharide in non-patent literature 3 and 4.

In this way, although the monosaccharide of isomerized sugar etc. is under the situation used as sweetening material in various beverages, mesh Before, there is suppression to be not only " people of diabetes borderline region ", also including other people people absorb isomerized sugar etc. monosaccharide it The elevated effect of blood glucose value afterwards, can be daily easy and safely while itself being low sweet taste~tasteless, Er Qieji The blood glucose value of continuous intake raises inhibitor according to known to the present inventor, has not been reported.

【Prior art literature】

【Patent document】

Patent document 1：Special open 2009-263655 publications

Patent document 2：Special open 2005-213227 publications

【Non-patent literature】

Non-patent literature 1：8th time big wheat food forum, on October 23rd, 2012

Non-patent literature 2：" starch chemistry ", volume 37, No. 2, the 107-114 pages (1990)

Non-patent literature 3：If woods etc., Japanese nutrition-grain association will, volume 46, No. 2, the 131-137 pages (1993)

Non-patent literature 4：If woods etc., Japanese food fiber research can will, volume 3, No. 1, the 13-19 pages (1999)

【The content of the invention】

【The invention technical task to be solved】

The present invention is intended to provide it is not limited to " people of diabetes borderline region " and has the blood of the people after suppressing monosaccharide intake The elevated effect of sugar value, itself is extensive to tasteless utilization scope with low sweet taste, can be daily easy and safely continues The blood glucose value rise inhibitor of intake and the oral composition containing it.

【Solve the technical solution of problem】

For solve the above subject repeat it is that sharp study is made great efforts as a result, the inventors discovered that, the applicant is previously in state Border discloses branch's alpha-glucans mixture disclosed in WO2008/136331 separate editions, specifically, using glucose as Sugar is formed, in the non-reducing end glucose residue of the straight-chain glucan of the glucose polymerization degree more than 3 through α-Isosorbide-5-Nitrae key connection On have the branched structure of the glucose polymerization degree more than 1 through the key connection beyond α-Isosorbide-5-Nitrae key, digested by isomaltose glucanase Branch's alpha-glucans mixture of isomaltose is generated, unexpectedly, not only suppresses the rise of postprandial plasma glucose level, also suppresses single The rise of blood glucose value after carbohydrate intake.Such opinion is complete unexpected opinion.The reason is that, due to above-mentioned branched alpha- Glucan mixture is the polymer for the glucose for having branch, in the point of display water-soluble dietary fiber is pasted with indigestible The mixture of the similar starch source of essence, in the same manner as the indigestible dextrins reported in non-patent literature 3 and 4, is considered pair Blood glucose value rise after monosaccharide intake does not show inhibition.However, people is by experimental verification, above-mentioned branch according to the present invention Alpha-glucans mixture, if people is absorbed, is not limited to " people of diabetes borderline region ", the blood glucose after monosaccharide intake The rise of value is significantly suppressed.Based on this new opinion, the present inventor establishes, itself is with low sweet taste to tasteless utilization scope Widely, " people of diabetes borderline region " effect is not limited to, the elevated blood for having the blood glucose value after suppressing monosaccharide intake Sugar value rise inhibitor and the oral composition containing it, thereby completing the present invention.

That is, the present invention is by providing to there is branch's alpha-glucans mixture of the feature of following (A) or even (C) as effectively Blood glucose value after the monosaccharide intake of component raises inhibitor and solves above-mentioned problem.

(A) using glucose as being formed sugar,

(B) have through the key connection beyond α -1,4 keys to positioned at the straight of the glucose polymerization degree more than 3 through α -1,4 key connections The branched structure of the glucose polymerization degree more than 1 of the non-reducing end glucose residue of one end of chain glucan,

(C) generation isomaltose is digested by isomaltose glucanase.

The branch's alpha-glucans mixture for having features described above is not only the safe edibility raw material of worry without side-effects, Low sweet taste~tasteless, when together being absorbed with monosaccharide, there is the elevated effect for the blood glucose value for suppressing people.So as to there is features described above (A) so (C) branch's alpha-glucans mixture as monosaccharide absorb after blood glucose value rise inhibitor active ingredient pole It is useful.

Furthermore by providing, together oral composition containing monosaccharide solves the present invention with above-mentioned blood glucose value rise inhibitor Certainly above-mentioned problem.

【Invention effect】

For the blood glucose value rise inhibitor of the present invention due to itself being low sweet taste~tasteless, utilization scope is extensive, can be unlimited Suppress the blood glucose value rise after monosaccharide intake in " people of diabetes borderline region ".In addition, the oral composition of the present invention Since the blood glucose value containing the present invention raises inhibitor, it easy and safely can effectively suppress intake monosaccharide by absorbing it The rise of blood glucose value afterwards.

【Brief description of the drawings】

【Fig. 1】Be show make subject intake be only made of glucose control sample when, and intake with glucose together During test specimens containing branch's alpha-glucans mixture, the average value of the increment of the blood glucose value in subject through time-varying The figure of change.

【Fig. 2】It has been displayed as the figure of the summary of the device made using the glucose absorption experiment of reversion small intestine.

【Embodiment】

The present invention is to be related to using the branch's alpha-glucans mixture for having the feature of following (A) or even (C) to be used as active ingredient Blood glucose value rise inhibitor invention.

(A) using glucose as being formed sugar,

(B) have through the key beyond α -1,4 keys and be connected to and be located at the glucose polymerization degree more than 3 through α -1,4 key connections The branched structure of the glucose polymerization degree more than 1 of the non-reducing end glucose residue of one end of straight-chain glucan,

(C) generation isomaltose is digested by isomaltose glucanase.

Described blood glucose value rise inhibitor refers to suppress the liter of the blood glucose value after the monosaccharide intake of people in this specification High agent.

Furthermore described intake refers to that people is taken into vivo in this specification, containing the intake by peroral route and by through pipe way Both the intake in footpath.

The blood glucose value rise inhibitor of the present invention preferably absorbs at the same time or before and after phase with monosaccharide.

The present invention blood glucose value rise inhibitor be as active ingredient contain above-mentioned branch's alpha-glucans mixture (with Under, it is known as " this branch alpha-glucans mixture ") form.This branch alpha-glucans mixture, as described later, can be by various systems The method of making obtains, and obtained this branch alpha-glucans mixture has been generally in various branched structures and glucose and has gathered The form of the mixture of most branch's alpha-glucanses of right (molecular weight), in existing technology, is divided one by one Branch alpha-glucans isolated is quantitatively impossible.Therefore, although can not be determined with the per molecule of branch's alpha-glucans every kind of The structure of branch's alpha-glucans, i.e., as the bonding pattern of the glucose residue of Component units and bonding order, but this branched alpha- The structure of glucan mixture can be made by the method for the method of the various physics generally used in this area, chemical method or enzyme For mixture general token.

Specifically, the structure of this branch alpha-glucans mixture, it is overall as mixture, by above-mentioned (A) or even (C) Characteristic present.That is, this branch alpha-glucans mixture is using glucose as the glucan (feature (A)) for being formed sugar, have through α- Key beyond 1,4 keys and the one end for being connected to the straight-chain glucan positioned at the glucose polymerization degree more than 3 through α -1,4 key connections Non-reducing end glucose residue glucose polymerization degree more than 1 branched structure (feature (B)).Furthermore institute in feature (B) Among " the non-reducing end glucose residue " said refers to the dextran chain through α-Isosorbide-5-Nitrae key connection, positioned at the end for not showing reproducibility The glucose residue at end, " key beyond α-Isosorbide-5-Nitrae key " as its name suggests, refer to the key beyond α-Isosorbide-5-Nitrae key.

Furthermore this branch alpha-glucans mixture digests generation isomaltose (feature (C)) by isomaltose glucanase. The digestion of described isomaltose glucanase is to instigate isomaltose glucanase to act on this branch alpha-glucans in feature (C) Mixture and hydrolyze.Isomaltose glucanase is to confer to the enzyme of enzyme number (EC) 3.2.1.94, even being to have to gather with α-Portugal Any bonding sample of α -1,2, α -1,3, α -1,4 and α -1,6 keys that the reduction end side of isomaltose structure in sugar abuts The enzyme for the feature that formula hydrolyzes.Preferably, using the different wheat in Arthrobacter globiformis (Arthrobacter globiformis) source Bud sugar dextranase (see, for example, Sawai et al., Agricultural and Biological Chemistry, volume 52, No. 2, page 495 page -501 (1988)).

Represented by the ratio of the isomaltose of every solids of the digest of isomaltose glucanase digestion generation in structure Into the different malt that can be hydrolyzed by isomaltose glucanase in the structure of branch's alpha-glucans of branch's alpha-glucans mixture The ratio of sugared structure, can be by feature (C), using the structure of this branch alpha-glucans mixture as mixture entirety, by the side of enzyme Method characterizes.

This branch alpha-glucans mixture is digested by isomaltose glucanase, with every solids of digest, in general, 5 Below 70 mass % of more than quality %, are preferably, below 60 mass % of more than 10 mass %, more preferably more than 20 mass % 50 Below quality % generates isomaltose, and the blood glucose value rise inhibition after being absorbed by monosaccharide is considered excellent, so as to be fitted Preferably use.

I.e., as described later, it is believed that this branch alpha-glucans mixture has generates different wheat by isomaltose glucanase digestion The feature of the structure of bud sugar and the rise inhibition of the blood glucose value after the monosaccharide intake by this branch alpha-glucans mixture It is deep related.That is, branch alpha-glucans of the isomaltose growing amount less than 5 mass % in isomaltose glucanase digestion Mixture is as the feature for having the structure close to the few maltodextrin of branched structure, on the contrary, in isomaltose glucan Isomaltose growing amount in enzymic digestion surpass 70 mass % branch's alpha-glucans mixture become have close to as with α -1,6 The feature of the structure of the glucan of the glucose polymer of key connection, due to being tied in branch specified in above-mentioned feature (B) Structure tails off, and the rise for being considered the blood glucose value after being absorbed with monosaccharide in the case of whole suppresses the feature of relevant structure gradually Weak, there are optimum range for the amount of the isomaltose digested by isomaltose glucanase.

In addition, the embodiment preferably as this branch alpha-glucans mixture, can enumerate by efficient liquid phase The water-soluble dietary fiber content that chromatograph (enzyme-HPLC methods) is obtained is feature (D) more than 40 mass %.

Obtain water-soluble dietary fiber content " high-performance liquid chromatograph method (enzyme-HPLC methods) " (hreinafter referred to as " enzyme- HPLC methods ".) refer to that the nutrition in May, 1996 Health and human services department bulletin the 146th represents the benchmark, " analysis method of nutritional ingredient etc. Deng (nutrition represents the method shown in benchmark other the 1st the 3rd column of table) " in the 8th, in the method described in " food fiber ", it is right Its outline illustrates then as follows.That is, by by sample by utilizing Thermostable aamylase, protease and glucoamylase A series of enzymatic treatment resolution process, by ion exchange resin from treatment fluid remove protein, organic acid, inorganic salts and modulate The sample solution of gel permeation chromatography.Next, for gel permeation chromatography, obtain in chromatogram do not digest glucan and The peak area of glucose, respective peak area, and separately, by conventional method, use the examination obtained by glucose-oxidase method Glucose amount in sample solution and calculate the water-soluble dietary fiber content of sample.It is furthermore in this specification in the whole text, " water-soluble Food fiber content ", as long as without special instruction, just refers to be contained by the water-soluble dietary fiber that above-mentioned " enzyme-HPLC methods " is obtained Amount.

Water-soluble dietary fiber content represents the content for the alpha-glucans not decomposed by alpha-amylase and glucoamylase, Feature (D) be by the structure of this branch alpha-glucans mixture, it is overall as mixture, the index characterized by the method for enzyme it One.

Furthermore as noted above it is believed that by isomaltose glucanase digest generation isomaltose structure feature with by The rise inhibition of blood glucose value after the monosaccharide intake of this branch alpha-glucans mixture is deeply related, the knot of this characteristic Structure part, certainly, the water-soluble dietary fiber content of this branch alpha-glucans mixture are higher, in other words, not by alpha-amylase It is just more and the content of branch's alpha-glucans of glucoamylase decomposition is more, it is not digested and reaches small intestine, is considered showing The rise inhibitory action of blood glucose value.So as to this branched alpha-Portugal as the active ingredient of the blood glucose value rise inhibitor of the present invention Oligosaccharide mixture, water-soluble dietary fiber content is more high the more preferred, and suitable water-soluble dietary fiber content is typically 40 matter More than % is measured, is further preferably more than 75 mass % more preferably more than 60 mass %.Suitable water-soluble dietary fiber content it is upper Limit is not particularly limited, if technically may, it is the higher the better, be preferably below 100 mass % or less than 100 mass %.

Furthermore as an embodiment preferably for this branch alpha-glucans mixture, there are following characteristics (E) and (F) Branch's alpha-glucans mixture, the feature can obtain by methylation analysis.

(E) ratio of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is in 1:0.6~1:4 scope,

(F) the total of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys accounts for the 55% of full glucose residue More than.

Methylation analysis, such as it is well known that referring in polysaccharide or oligosaccharides, the bonding sample as definite its monose of composition The method of formula generally widely used method (Ciucanu et al., Carbohydrate Research, volume 131, No. 2, 209-217 pages (1984)).When methylation analysis to be applied to the analysis of bonding pattern of the glucose in glucan, first, make All free hydroxymethylation in the glucose residue for forming glucan, next, the Portugal of hydrolysis exhaustive methylation gathers Sugar.Next, the glucose that methylates hydrolyzed by also reason is and the glucitol that methylates as the different head dummy of cancellation, then Person, free acetylating hydroxyl groups in the glucitol that makes to methylate at this and obtain partial methylation glucitol acetic acid esters (again Person, sometimes by " partial methylation glucitol acetic acid esters " simply collectively referred to as " part methyl compound ").Pass through the part that will be obtained Methide gas chromatographic analysis, is bonded style source in the various parts of each different glucose residues in glucan The percentage (%) of the peak area for all parts methide that methide can be accounted in gas chromatogram with peak area represents.Into And can be determined from this peak area % in the glucan bonding pattern different glucose residues there are ratio, i.e., each Portugal Polyglycoside key there are ratio.Refer to the peak face in the gas chromatogram of methylation analysis relative to " ratio " of part methyl compound Long-pending " ratio ", " the area % " in the gas chromatogram of methylation analysis is referred to relative to " % " of part methyl compound.

" glucose residue of α -1,4 keys " in above-mentioned (E) and (F) refers to only through the carbon atom bonding with 1 and 4 Hydroxyl and the glucose residue that is bonded with other glucose residues, in methylation analysis, as 2,3,6- trimethyls-Isosorbide-5-Nitrae, 5- triacetyl glucitols are detected.In addition, " α -1, the glucose residue of 6 keys " in above-mentioned (E) and (F) refer to only pass through with The hydroxyl of the carbon atom bonding of 1 and 6 and the glucose residue being bonded with other glucose residues, in methylation analysis, It is detected as 2,3,4- trimethyl -1,5,6- triacetyl glucitols.

The glucose residue and α -1 of the α obtained by methylation analysis-Isosorbide-5-Nitrae key, the ratio of the glucose residue of 6 keys and, Relative to the glucose residue and α -1 of α-Isosorbide-5-Nitrae key, the ratio of the full glucose residue of the glucose residue of 6 keys can be by this branch The structure of alpha-glucans mixture is overall as mixture, is used as one of index characterized by chemical method.

Above-mentioned (E) " ratio of the glucose residue of α -1,4 keys and the glucose residue of α -1,6 keys be in 1:0.6~1:4 Scope " regulation refer to this branch alpha-glucans mixture for methylation analysis when, the 2 of detection, 3,6- trimethyls- The ratio of 1,4,5- triacetyls glucitol and 2,3,4- trimethyl -1,5,6- triacetyl glucitols is in 1:0.6~1:4 model Enclose.In addition, above-mentioned (F) " glucose residue and α -1 of α-Isosorbide-5-Nitrae key, to account for full glucose residual by the glucose residue of 6 keys total The regulation of more than the 55% of base " refers to this branch alpha-glucans mixture, in methylation analysis, 2,3,6- trimethyls-Isosorbide-5-Nitrae, 5- triacetyls glucitol and the total of 2,3,4- trimethyl -1,5,6- triacetyl glucitols account for partial methylation glucitol More than the 55% of acetic acid esters.In general, starch is without only in the glucose residue of 1 and 6 bonding, and the glucose of α-Isosorbide-5-Nitrae key Residue account for it is more than half in full glucose residue, so the important document of above-mentioned (E) and (F) mean that this branch alpha-glucans mixture has From the complete different structure of starch.

Such as provided in the feature of above-mentioned (E) and (F), this branch alpha-glucans mixture, in a preferable embodiment In, it is typically that certain degree has " α -1, the glucose residue of 6 keys " being not present in starch, but with high blood glucose value rise Inhibition because have a more complicated branched structure can expect high blood glucose value rise inhibition, is removed as if necessary α-Isosorbide-5-Nitrae key and α -1, outside 6 keys, preferably also α -1,3 keys and α -1,3,6 keys.Wherein, " α -1,3,6 keys " refer to " with 1, 3 positions of the hydroxyl of 3 and 6 are bonded (α -1,3,6 bondings) glucose residue with other glucose ".This branch alpha-glucans Mixture, contains α -1,3 bondings and α -1,3,6 bondings are in bonding in order to there is more complicated branched structure with arbitrary ratio Can, for example, α -1, the glucose residues of 3 bondings are preferably more than the 0.5% of full glucose residue less than 10%.It is in addition, well-behaved Branch alpha-glucans mixture, α -1, the glucose residue of 3,6 bondings are preferably more than the 0.5% of full glucose residue.

It is above-mentioned " α -1,3 be bonded glucose residue be more than the 0.5% of full glucose residue can pass through less than 10% " by When this branch alpha-glucans mixture is for methylation analysis, 2,4,6- trimethyls -1,3,5- triacetyl glucitols are with portion More than the 0.5% of the glucitol acetic acid esters that methylates is divided to exist less than 10% and confirm.In addition, above-mentioned " α -1, the Portugal of 3,6 bondings Grape saccharide residue is more than the 0.5% of full glucose residue " can by this branch alpha-glucans mixture, in methylation analysis, Four acetyl glucitols of 2,4- dimethyl -1,3,5,6- are with more than 0.5% deficiency of partial methylation glucitol acetic acid esters 10% exists and confirms.

This branch alpha-glucans mixture also can by weight average molecular weight (Mw) and weight average molecular weight (Mw) divided by Value (Mw/Mn) characterization of number average molecular weight (Mn).Weight average molecular weight (Mw) and number average molecular weight (Mn), for example, can Obtained using size exclusion chromatography etc..In addition, calculate structure cost branch α-Portugal because of weight average molecular weight (Mw) can be based on The average glucose degree of polymerization of branch's alpha-glucans of oligosaccharide mixture, this branch alpha-glucans mixture also can be by average grapes Sugared degree of polymerization characterization.The average glucose degree of polymerization can subtract 18 from weight average molecular weight (Mw), divided by be used as glucose residue amount 162 and obtain.This branch alpha-glucans mixture that active ingredient as blood glucose value rise inhibitor uses, its average Portugal Grape sugar polymerization is typically 8~500, be preferably 15~400, more preferably 20~300 be suitable.Furthermore branch's alpha-glucans Mixture, the average glucose degree of polymerization is bigger, viscosity increase, the point that the average glucose degree of polymerization is smaller, viscosity more diminishes, shows Show the property same with common glucan.Therefore, can according to the present invention blood glucose value rise inhibitor embodiment, suitably Selection has this branch alpha-glucans mixture of the average glucose degree of polymerization for the viscosity for being suitable for requirement and uses.

As the Mw/Mn of weight average molecular weight (Mw) divided by the value of number average molecular weight (Mn), mean closer to 1 The deviation of the glucose polymerization degree of branched alpha-dextran molecule of the branch's alpha-glucans mixture formed is small.As blood glucose This branch alpha-glucans mixture that the active ingredient of value rise inhibitor uses, although as long as Mw/Mn usually less than 20, It can use without problems, but preferably less than 10, more preferably less than 5 be suitable.Furthermore requiring to provide specific Portugal During branch's alpha-glucans mixture of the grape sugar degree of polymerization, for Mw/Mn close to 1, the deviation of glucose polymerization degree is smaller the more preferred.

As long as this branch alpha-glucans mixture has the feature of above-mentioned (A) or even (C), any method system just can be also used Make.For example, make the non-reducing end grape of the straight-chain glucan of the oriented glucose polymerization degree more than 3 through α-Isosorbide-5-Nitrae key connection Saccharide residue imports the enzyme effect of the effect of the branched structure of the glucose polymerization degree more than 1 connected through α -1,6 keys in starchiness Obtained from branch's alpha-glucans mixture can suitably utilize in an embodiment of the present invention, as an example preferably, can lift Phlorose based transferase disclosed in International Publication No. of sening as an envoy to WO2008/136331 separate editions acts on starchiness and obtains Branch's alpha-glucans mixture.In addition, in addition to above-mentioned phlorose based transferase, since combination maltotetraose generation is formed sediment The amylase of powder enzyme (EC3.2.1.60) etc., or the starch debranching enzymes of isoamylase (EC 3.2.1.68) etc., Ze Keshiben branches Alpha-glucans mixture is degraded, molecular weight, glucose polymerization degree etc. can be adjusted to desired scope.Furthermore pass through connection With cyclomaltodextrin glucanotransferase (CGTASE) (EC2.4.1.19), or Q-enzyrne (EC 2.4.1.18), in special open 2014- Having disclosed in No. 054221 publication makes the α -1,4 glucans α -1,6 of the degree of polymerization more than 2 be transferred to the grape of amylaceous inside The active enzyme of saccharide residue, can also make branch's alpha-glucans of structure cost branch alpha-glucans mixture further hyperbranched, Improve the water-soluble dietary fiber content of this branch alpha-glucans mixture.Again make the saccharic hydrolase of glucoamylase etc. For the branch's alpha-glucans mixture so obtained, gather as the branched alpha-Portugal for further improving water-soluble dietary fiber content Sugared mixture is also random, by making glycosyl trehalose generate enzyme (EC 5.4.99.15) effect, is gathered to branched alpha-Portugal is formed The reduction end of branch's alpha-glucans of sugared mixture imports trehalose structure, or also can reduce branch's alpha-glucans by hydrogen addition Reduction end grade and reduce the reducing power of branch's alpha-glucans mixture, in addition, by carry out by size exclusion chromatography Deng classification separation, it is also random that obtaining, which has branch's alpha-glucans mixture of desired molecular weight,.

As long as the amount of this branch alpha-glucans mixture in making to be contained in the blood glucose value rise inhibitor of the present invention with Monosaccharide plays desired blood glucose value rise inhibitory action at the same time or when the front and rear intake of phase, is just not particularly limited, by this branch Alpha-glucans mixture with 1~100 mass %, be preferably that 3~100 mass %, the more preferably scope of 5~100 mass % contain Have.In addition, the blood glucose value rise inhibitor of the present invention, also can be according to need in addition to this branch alpha-glucans mixture Will, by selected from water, mineral, the one kind or two or more component for spices, stabilization agent, excipient, extender, pH regulators etc. With 0.01~50 mass %, it is preferably that the ratio of 0.1~40 mass % suitably coordinates and utilizes.

As long as the amount intake that the blood glucose value rise inhibitor of the present invention plays the action effect of blood glucose value rise inhibitor is Can, intake is without particular limitation, for example, the intake of this branch alpha-glucans mixture as active ingredient, usually with As adult (weight 60kg) every 1 time, the scope of 0.5~100g, be preferably the scope of 1~50g, more preferably 1.5~10g Scope, further preferably be 3~8g scope mode, by the present invention blood glucose value raise inhibitor, directly, alternatively, be dissolved in water, The beverage of tea, coffee etc. and absorb, or be added in Foods or drinks and absorb.Alternatively, active ingredient can also be used as This branch alpha-glucans mixture intake relative to monosaccharide total intake as 0.5~30 mass % scope, The mode of the more preferably scope of 5~15 mass % absorbs the blood glucose value rise inhibitor of the present invention.Furthermore certainly also can be by this Blood glucose value rise inhibitor intake before and after the intake of Foods or drinks of invention.

The blood glucose value rise inhibitor of the present invention can be used as powdered, granular, graininess, liquid, paste, white shape, sheet, Capsule shape, caplet shape, software capsule shape, lozenge shape, bar-shaped, tabular, bulk, pill shape, solid-like, gelatinous, glue, natural gum The suitable form of shape, wafer-like, biscuit shape, maltosemalt sugar shape, masticatory pattern shape, syrupy shape, strip etc..In addition, the blood glucose value of the present invention Rise inhibitor be not only medicine or quasi drug, can with specific health food, feature represent food, dietary supplement, Or the food absorbed for the purpose of the habit disease that prevents or make the life better of healthy food etc. coordinates.Food as cooperation Concrete example, can enumerate soda, milk beverage, fruit syrup, sports drink, vinegar beverage, soymilk, the beverage containing iron, The food of the beverage of sour milk beverage, green tea, black tea, cocoa, coffee etc., rice, congee, bread, noodles, soup, sauce, yogurt etc. Product, soft sweets, hard candy, natural gum, jelly, biscuit, soft biscuit, thin pancake, graupel cake, the candied cake of popped rice, asks fertilizer, cake class, fern cake, steamed stuffed bun, outer youth Cake, filling class, red bean jelly, water red bean jelly, bright and beautiful jade, jelly, pectin jelly, the main chastity of family are good, biscuit, pretzel, group, pudding, cream, custard Frost, cream foam dessert, Waffle, steamed sponge cake, pancake, muffin, doughnut, chocolate, Gan Na are permitted, cereal bar, mouth are fragrant Dessert, ice cream, sherbet, the Gelato of sugar, caramel, nougat, flower cream, peanut cream, fruit cream, jam, marmalade etc. Furthermore Deng the freezing point heart, soy sauce, sauce powder, sauce, powder sauce, fermented glutinour rice, eggplant cucumber sauce, seaweed salt, mayonnaise, baste, Vinegar, three glasss of vinegar, powder sushi vinegar, the element of China, tempura dip, noodles flavoring are sauce, tomato sauce, catsup, roasting The sauce of meat, the sauce of roast chicken, dry deep-frying powder, tempura powder, curried soup, stewed element, the element of soup, the element of meat soup, compound seasoner, The various flavorings or conditioning processed goods of Japanese sweetener wine, new Japanese sweetener wine, table sugar, coffee candy etc..Furthermore blood of the invention Sugared value raise inhibitor also can with the liquor for preventing or improving (treatment) lifestyle disease, syrup, through pipe nutrition The medicament of the form of agent, lozenge, capsule, lozenge, sublingual dose, granule, powder, pulvis, emulsion, spray etc. coordinates.Again Person, blood glucose value of the invention rise inhibitor can also coordinate with pet food or feed, the bait of the animal consumption beyond people.

In addition, the blood glucose value rise inhibitor of the invention containing this branch alpha-glucans mixture serves not only as direct blood Sugar value rise inhibitor, in addition to the blood glucose value rise inhibitor of the present invention, can add monosaccharide again and be used as oral combination Thing uses.The oral composition of the present invention containing blood glucose value due to raising inhibitor, due to containing monose in oral composition Class, also can inhibit the rise of the blood glucose value after intake, even there is the elevated people of care about blood glucose value, can also not mind containing monose The benefit absorbed to class.

For in passing, as the monosaccharide used in the oral composition of the present invention, can enumerate makes monose in people Class intake after the elevated effect of blood glucose value monose, particularly, with food coordinate monose, for example, selected from glucose, fruit Sugar, more than a kind of galactolipin, be exactly wherein, using as the different of the mixture of glucose, fructose and glucose and fructose Structureization sugar, be particularly, when glucose is together coupled to the oral composition of the present invention with blood glucose value rise inhibitor, more appropriately Play by the present invention blood glucose value rise inhibitor blood glucose value rise inhibition and it is preferred that.

In the oral composition of the present invention, the blood glucose value rise inhibitor of the present invention is raised using playing as blood glucose value The amount of the action effect of inhibitor coordinates, use level is without particular limitation, for example, relative to containing in oral composition The total amount of monosaccharide, this branch alpha-glucans mixture as active ingredient is preferably with the model for synthesizing 0.5~30 mass % Enclose, be more preferably the scope of 5~15 mass %.Furthermore this branch alpha-glucans mixture as active ingredient can match somebody with somebody synthesis To be grown up (weight 60kg) every 1 time, in general, the scope of 0.5~100g, be preferably 1~50g scope, more preferably 1.5~ The scope of 10g, further preferably for the scope of 3~8g, to absorb be suitable.

, can also be relative to the total amount of oral composition in addition to mentioned component in the oral composition of the present invention And 0.01~50 mass %, it is preferably that the ratio of 0.1~40 mass % suitably coordinates selected from water, physiological saline, sweetening material, egg White matter, peptide, polyphenol, mineral, antibacterial material, enzyme, the polysaccharide of indigestible, colouring matter, spices, thickener, stabilization agent, tax Shape agent, extender, pH regulators etc. it is one kind or two or more.

The oral composition of the present invention can be used as powdered, granular, graininess, liquid, paste, white shape, sheet, capsule Shape, caplet shape, software capsule shape, lozenge shape, bar-shaped, tabular, bulk, pill shape, solid-like, gelatinous, glue, gumminess, The suitable form of wafer-like, biscuit shape, maltosemalt sugar shape, masticatory pattern shape, syrupy shape, strip etc..

The oral composition of the present invention also can be as needed, from the non-oral administration method applied through pipe etc. to stomach or digestion Pipe is applied.

Hereinafter, the present invention is described in more detail based on experiment.

In following experiment 1, recorded using according to the embodiment 5 of International Publication No. WO2008/136331 separate editions Method manufacture branch's alpha-glucans mixture.That is, the method recorded according to above-described embodiment 5, to 27.1 mass % corns In addition liquefied starch (percent hydrolysis 3.6%) plus sodium hydrogensulfite add calcium chloride to becoming to 0.3 mass % of ultimate density is become After ultimate density 1mM, 50 DEG C are cooled to, adds 11.1 units by International Publication No. WO2008/136331 per 1g solids to it Bacillus circulans (Bacillus circulans) PP710 (FERM of method modulation described in the embodiment 1 of number separate edition BP-10771) the concentration crude enzyme liquid of the phlorose based transferase in source, furthermore, 50 DEG C, pH6.0 effect 68 it is small when.Should Reaction solution is after 80 DEG C are kept for 60 minutes, cooling, by the filtrate being obtained by filtration according to conventional methods, with activated carbon decolorizing, by H Type and OH type ion exchange resin desalinations and purify, then concentrate, branch's alpha-glucans mixture for being spray-dried and manufacturing is following Used in experiment 1.Furthermore by obtained branch's alpha-glucans mixture by International Publication No. WO2008/136331 separate editions Paragraph 0079,0080 described in isomaltose glucanase digestion trial method, alpha-glucosidase and glucoamylase When methylation assay described in digestion trial method, paragraph 0076~0078 is analyzed, there is the feature of following (a) or even (c).

(a) using glucose as being formed sugar,

(c) digested by isomaltose glucanase, the solids per digest generates 35 mass % isomaltoses.

In addition, when obtained branch's alpha-glucans mixture is analyzed by above-mentioned enzyme-HPLC methods, above-mentioned branch's alpha-glucans Mixture, with the exception of the features described above, there is the feature of following (d), furthermore, from the analysis result by above-mentioned methylation assay, Confirmation has the feature of following (e) or even (h).

(d) water-soluble dietary fiber content is 82.9 mass %,

(e) ratio of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is 1:2.1

(f) the total of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is full glucose residue 73.8%.

(g) glucose residue that α -1,3 is bonded is the 2.1% of full glucose residue.

(h) glucose residue that α -1,3,6 is bonded is the 5.6% of full glucose residue.

Furthermore the paragraph by branch's alpha-glucans mixture by International Publication No. WO2008/136331 separate editions During the molecular weight distribution analysis of the gel filtration HPLC described in 0081, its weight average molecular weight (Mw) is 5,000 dalton (be scaled the average glucose degree of polymerization then about 30), Mw/Mn are 2.1.

As described above, the branch's alpha-glucans mixture used in this experiment is using glucose as sugar is formed, have through Key beyond α -1,4 keys and be connected to the straight-chain glucan of the glucose polymerization degree more than 3 connected through α -1,4 keys it is non-also The branched structure of the glucose polymerization degree more than 1 of former terminal glucose saccharide residue, has different by isomaltose glucanase digestion generation The feature of above-mentioned (A) or even (C) of maltose.In addition, the branch alpha-glucans mixture used in this experiment meet by Isomaltose glucanase digests, and isomaltose is generated with below the 70 mass % of more than 5 mass % of every solids of digest Feature, water-soluble dietary fiber content be more than 40 mass % above-mentioned (D) feature and, the glucose residue of α-Isosorbide-5-Nitrae key 1 is in the ratio of the glucose residue of α -1,6 keys:0.6~1:4 scope, the glucose residue and α -1 of α-Isosorbide-5-Nitrae key, 6 keys Total more than 55% above-mentioned (E), feature of (F) for accounting for full glucose residue of glucose residue.

Furthermore above-mentioned branch's alpha-glucans mixture is α -1, the glucose residue of 3 bondings is in full glucose residue More than 0.5% scope less than 10%, α -1, the glucose residue of 3,6 bondings are in more than the 0.5% of full glucose residue Scope.

In following experiment, in order to study shadow of the branch's alpha-glucans mixture in glucose uptake to blood glucose value Ring, implement glucose load experiment.

To the blood glucose value on an empty stomach in addition to the person of the abnormal experience of the glucose metabolism energy for having diabetes etc. (before intake Blood glucose value) be general 80~110mg/dL healthy 13 people of men and women subject to the control sample that will be only made of glucose 50g is dissolved in water (with reference to table 1) as the aqueous solution of 200mL, is absorbed within 5 minutes.Before intake, intake after 30,45,60, 90th, the meter of 120 minutes 6 times, take a blood sample from the finger tip of subject, use ph test paper ph " Accu-ChekAvivaStripF " (Roche Diagnostics Co., Ltd. sells) and own blood glucose analyzer " Accu-ChekAvivaNano " (Roche Diagnostics Co., Ltd. sells) and measure blood glucose value.Furthermore as glucose, use commercially available powder glucose (business The name of an article " Fuji Crystar " plus the sale of rattan Chemical Co., Ltd.).

Next, from above-mentioned experiment after more than one week, for the subject of above-mentioned 13 people, taken the photograph with control sample Situation about taking similarly absorb by with test specimens 55g (with reference to table 1) of the glucose together containing branch's alpha-glucans mixture Water is dissolved in as the aqueous solution of 200mL, after intake, is similarly taken a blood sample, the change to blood glucose value is measured.

【Table 1】

* glucose and branch's alpha-glucans mixture are dissolved in water, 200mL is used as using full dose.

For above-mentioned 13 people of subject control sample absorb when and test specimens absorb when, be each made relative to intake Afterwards before elapsed time plotting intake, 30 after intake, the coordinate of the average value of the increment of the blood glucose value of 45,60,90,120 minutes Figure, is shown in Fig. 1.In addition, calculating the area under the curve (AUC) of the average value of the increment of blood glucose value per sample, table 2 is shown in.Furthermore AUC when AUC divided by control sample when the influence of branch alpha-glucans mixture intake is absorbed by test specimens are absorbed and obtain The AUC ratios evaluation arrived, as a result in table 2 and remembers.

【Table 2】

*：(test specimens AUC/ control sample AUC) × 100

As shown in Figure 1, (symbol zero of Fig. 1) compares when being absorbed with control sample, (the symbol of Fig. 1 when test specimens absorb ●) rise of the increment of blood glucose value is suppressed.In addition, as shown in table 2, AUC when control sample absorbs is 99.0, with this phase Right, AUC when test specimens absorb down to 86.6, AUC ratios is 87.4%.Confirmed from result, branch's alpha-glucans mixing If thing is together absorbed with the glucose as monose, with only absorbing glucose when ratio, significantly suppress the rise of blood glucose value.

For in passing, this branch alpha-glucans mixture with Starch Fraction decomposition product (maltodextrin) when together absorbing Suppress the rise of blood glucose value in the International Publication No. WO2008/136331 separate editions by the applicant identical with the applicant It is open.Above-mentioned experimental result represents, branch's alpha-glucans mixture, when being not only intake Starch Fraction decomposition product, even in taking the photograph Take the rise for also suppressing blood glucose value during monosaccharide.

On the one hand, as previously mentioned, in the art as the widely used indigestibility of water-soluble dietary fiber raw material Property dextrin, the rise for reporting the blood glucose value after the intake for glucose and fructose as monose do not show inhibition (with reference to non-patent literature 3 and 4).This branch alpha-glucans mixture and indigestible dextrins, although being the grape that has branch The polymer of sugar, is that the point of the mixture for the starch source for having water-soluble dietary fiber is common, after glucose uptake The elevated influence of blood glucose value is not both the opinion for the extremely interest depth for overturning conventional common technical knowledge, this branch alpha-glucans mixes Compound, the point of the extraction of the glucose in organism is at least significantly suppressed, it is believed that its action effect and indigestible dextrins There is notable difference.

This branch alpha-glucans mixture is due to playing the rise suppressed as the blood glucose value after the glucose uptake of monose Action effect, even worrying to cause the rise of blood glucose value all the time, the food containing monosaccharide, for example, containing grape Various foods of the monosaccharide of sugar, fructose, isomerized sugar etc. as sweetening material, as long as this branch alpha-glucans mixture is matched somebody with somebody Close, can expect to can inhibit the rise of the blood glucose value after absorbing.If using this branch alpha-glucans mixture, can expand can be without blood The scope of the food absorbed to the elevated mystery of sugared value, this branch alpha-glucans mixture, inhibitor is raised as blood glucose value, It is more favourable than very widely used today indigestible dextrins in the field of food containing monosaccharide, especially field of beverage Ground utilizes.

In following experiment 2, press down as the blood glucose value after the bright monose by this branch alpha-glucans mixture absorbs is solved One means of the mechanism of making, by influence of this branch alpha-glucans mixture to the glucose absorption in small intestine by using big Mouse invert small intestine verification experimental verification, and with the results contrast of the indigestible dextrins on progress.

In order to verify the mechanism of the blood glucose value of this branch alpha-glucans mixture in people rise inhibitory action, using big Mouse inverts small intestine and inquires into the influence to the glucose absorption in small intestine.As this branch alpha-glucans mixture, using with The identical standard items used in experiment 1, as indigestible dextrins, use commercially available indigestible dextrins (trade name " Fibersol2 ", SongGu Chemical Industrial Co., Ltd sell).

For in passing, (it see, for example, positive man of virtue and ability of earth house etc., the Portugal on rat reversion small intestine using the experiment of reversion small intestine The research that grape sugar absorbs：Through when observation exploitation Japan digester disease association magazine, volume 80,1138-1143 pages (1983 Year)) refer to study for the transmission mechanism of the specific part to enteron aisle, extract its part and invert, ligation both ends, be allowed to The method that the transmission of material is inquired into as cryptomere, such as buffer solution is put among capsule, while containing object material (this Glucose in experiment) experimental liquid in lead to 95%O₂- 5%CO₂Gas, while being incubated to capsule.To in the experimental liquid Object material is absorbed from mucous membrane of small intestine, is migrated to the amount of the serosa side of intracapsular side and is measured, and evaluation absorbs.Invert enteron aisle and Make mucous membrane lateral outer, since epithelial cell requires substantial amounts of oxygen, make its supply easily by as the first reason.In this experiment In, in vitro through when measure by small intestine glucose absorption.

Wistar rats (hero, 7 week old, the sale of CLEA Co., Ltd. of Japan) are bought, were raised at 1~3 week to standard feed Educate and tame.Next, make the rat go on a hunger strike 4 it is small when after, by under anaesthetized with pentobarbital midsection opening abdomen and Small intestine is gathered, (stage casing of small intestine is in the Treitz ligaments of the rat small intestine than collection, small intestine is tied to the wall of side in the back of the body The structure of the ligament sample of (posterior abdominal wall)) lower side (ileum side) cut out about 16cm to 24cm position length about 8cm part Small intestine, uses in test.

Glucose absorption experiment using rat reversion small intestine is the experimental rig shown in Fig. 2 to be made, according to following Order is implemented.

Hereinafter, for the summary of experimental rig, illustrated based on Fig. 2.Injection tube (the symbol d) of Fig. 2 is held with plastics 20mL Used as container, gas exhaust aperture is set on the top of injection tube d, and (the symbol b) of Fig. 2, is installed in the lower end of injection tube d Gas backstreaming pipe (the symbol h) of Fig. 2.In rubber bolt (the central portion perforate of the symbol a) of Fig. 2, the plastics of insertion cut-out tip 1mL chips processed (the symbol c) of Fig. 2, in the tip of the chip c, the reversion small intestine of installation ligation one end (the symbol e) of Fig. 2 and Ligation.It is inserted into from the upper end of injection tube d, as experimental rig.

(1) will only glucose, glucose and branch's alpha-glucans mixture or, glucose and indigestible dextrins are each From Krebs-Ringer bicarbonate buffers (pH7.4) (hreinafter referred to as " KRB buffer solutions ") are dissolved in, following 3 kinds are modulated Given the test agent solution.

Control group：Only glucose (180mg/dL, 10mM)；

Branch's alpha-glucans mixture addition group：To the alpha-glucans mixing of glucose (180mg/dL, 10mM) addition branch Thing (60mg/dL)；

Indigestible dextrins addition group：To glucose (180mg/dL, 10mM) addition indigestible dextrins (60mg/dL)；

(2) injection tube (symbol d) injection 20mL (the above-mentioned 3 kinds of test samples of symbol g) of Fig. 2 of Fig. 2 are held to plastics 20mL Any one of product solution, is put into 37 DEG C of Water Tank with Temp.-controlled and (the symbol h) of Fig. 2, is passed through 95%O from gas backstreaming pipe₂- 5% CO₂Gas, carries out gas backstreaming.

(3) by the inside of the rat small intestine obtained in 2-1 is tested with being passed through 95%O₂- 5%CO₂The cold KRB bufferings of gas Liquid cleans.

(4) reversion rod (not shown) is inserted into a little to the peri position end of enteron aisle, while it is pressed into reversion rod to small enteral, one Side inverts, as reversion small intestine.

(5) by the way that to the reversion small intestine taken off from reversion with rod, (the plastics 1mL of tip is cut off in the symbol e) insertions of Fig. 2 Chip (symbol c)-ligation of Fig. 2 and install, ligature from peri position end about at 5cm.

(6) the chip c for installing reversion small intestine e is arranged on to the inside of injection tube d, with micropipettor from the upper of chip c KRB buffer solutions 0.5mL (the symbol f) of Fig. 2 inject to reversion tunica serosa intestini tenuis side (inner side of reversion small intestine c) in portion.Make in this time point Start for experiment (incubation).Furthermore before experiment and in experiment, by 95%O₂- 5%CO₂Gas is passed through mucous membrane side (reversion small intestine c Outside) given the test agent solution g.

(7) mucous membrane side, serosa side are incubating the time point of 0,30,60,90,120 minute by the micro shiftings of each 5 μ L of solution Liquid device samples, and uses glucose quantitation kit (trade name " glucose CII tests WAKO ", Wako Pure Chemical Industries, Ltd. Sale) and quantifying glucose.

(8) each test sample group is each tested 6 times, and the inspection of data, is compared with Tukey-Kramer methods.With danger Dangerous rate is used as " there were significant differences " less than 0.05.

Using reversion small intestine glucose absorption experiment in, through when measure serosa side concentration of glucose the results are shown in Table 3.

【Table 3】

*：Average value ± standard deviation, n=6, in different intersymbols there were significant differences (p<0.05)

As shown in table 3, learn in control group (only glucose), the concentration of glucose of serosa side divides in on-test 30 Clock, 60 minutes, 90 minutes, the time point of 120 minutes, each up to 31.5 ± 10.9mg/dL, 96.2 ± 17.8mg/dL, 155.2 ± 23.0mg/dL, 202.8 ± 27.5mg/dL, concentration of glucose rise with time going by, inverts intestinal absorption mucous membrane The glucose of side, moves to serosa side.On the one hand, in branch's alpha-glucans mixture addition group, the glucose of serosa side is dense Degree at the time point of 30 minutes on-tests, 60 minutes, 90 minutes, 120 minutes, be respectively 13.3 ± 6.6mg/dL, 40.4 ± 26.5mg/dL, 71.7 ± 40.6mg/dL, 102.7 ± 58.3mg/dL are significantly lower compared to the control group in each time showing Value.In addition, indigestible dextrins addition group is shown and result of the control group without big difference.Result more than learns, branched alpha-Portugal Oligosaccharide mixture plays the role of suppressing the glucose absorption in mucous membrane of small intestine, and on the other hand, indigestible dextrins press down without absorption Make and use.

Further, since the enteral amount of buffer of reversion after incubating 120 minutes often tests not necessarily certain, experiment knot Serosa side amount of buffer during beam is multiplied by concentration of glucose and calculates the full glucose amount of reversion tunica serosa intestini tenuis side.Control group, divide Branch alpha-glucans mixture addition group and, the enteral full glucose amount of the reversion of indigestible dextrins addition group is individually 1.15 ± 0.27mg, 0.47 ± 0.34mg and, 0.93 ± 0.33mg, in the same manner as the situation of the concentration of glucose shown in table 3, It is significantly fewer than the glucose amount of absorption with control group in branch's alpha-glucans mixture addition group.On the one hand, in absorption In full glucose amount, indigestible dextrins addition group and control group are also without big difference.

As understood from the above, branch's alpha-glucans mixture addition group is with compareing (only glucose) group and indigestible Dextrin addition group ratio, the uptake of glucose is significantly few when 90~120 minutes after sugar loads.On the one hand, indigestible is pasted Smart addition group is with control group without big difference.Result more than judges that branch's alpha-glucans mixture has the grape in small intestine The absorption inhibitory action of sugar, on the one hand, absorption inhibitory action of the indigestible dextrins without glucose.For in passing, indigestible Dextrin does not show in the glucose absorption experiment using reversion small intestine absorbs inhibitory action in document (if woods is luxuriant, " indigestible is pasted Influence of the essence to sugar tolerance ", day endocrine can will, volume 68,623-635 pages (1992)) in also have report.

From the result confirmation of experiment 2, this branch alpha-glucans mixture suppresses the extraction of the glucose in small intestine.Therefore Confirm, the blood glucose value rise in the glucose uptake of the people by this branch alpha-glucans mixture suppresses to be by small intestine The absorption of glucose suppresses.Learnt by this experiment, monosaccharide of the indigestible dextrins in organism does not show blood glucose when absorbing Value rise inhibitory action, on the other hand, this branch alpha-glucans mixture show the one of blood glucose value rise inhibitory action because being pair The difference of the effect of glucose absorption in small intestine.

How this branch alpha-glucans mixture acts on and to suppress extraction of the glucose in small intestine to blood unknown, but Have through the key connection beyond α-Isosorbide-5-Nitrae key to this branch alpha-glucans mixture, the glucose polymerization degree connected through α-Isosorbide-5-Nitrae key The branched structure of the glucose polymerization degree more than 1 of the non-reducing end glucose residue of more than 3 straight-chain glucan, has by different The feature of the structure of maltose dextranase digestion generation isomaltose, is more preferably digested by isomaltose glucanase, had The feature of the structure of the solids generation 70 following isomaltoses of mass % of more than 5 mass % per digest is estimated to be for hair Wave function necessity.

Furthermore due to branched alpha of the isomaltose growing amount in being digested in isomaltose glucanase less than 5 mass %- Glucan mixture is close to the structure of the few maltodextrin of branched structure, is estimated to be for the glucose in small intestine Extraction influence it is small.On the one hand, the isomaltose growing amount in isomaltose glucanase digestion surpasses point of 70 mass % Branch alpha-glucans mixture becomes close to as with α -1, the structure of the glucan of the glucose polymer of 6 key connections, on the contrary, Since branched structure becomes dull on the contrary, the influence for being estimated to be the extraction to the glucose in small intestine diminishes.In addition, by this The water-soluble dietary fiber content that high-performance liquid chromatograph method (enzyme-HPLC methods) is obtained among branch's alpha-glucans mixture is 40 More than quality % due to itself in small intestine indigestibility absorb and be estimated to be more preferably.

On the one hand, indigestible dextrins do not suppress the extraction of the glucose in small intestine.Although indigestible dextrins for Inhibition is shown in the rise of the blood glucose values after saccharic intake more than disaccharides, as the blood glucose value after being absorbed for monosaccharide Rise does not show the mechanism of action of inhibition, is estimated to be since indigestible dextrins suppress what is interlocked with disaccharides catabolic enzyme The extraction of glucose.

Hereinafter, the present invention is described in more detail based on embodiment.But the present invention limits from these embodiments.

【Embodiment】

【Embodiment 1】

According to the method described in the embodiment 5 of International Publication No. WO2008/136331 separate editions, branched alpha-Portugal is modulated Oligosaccharide mixture powder.Furthermore obtained branch's alpha-glucans mix powder has the feature of following (a) or even (g).

(a) using glucose as being formed sugar,

(c) digested by isomaltose glucanase, isomaltose generated with the 35 mass % of every solids of digest,

(d) water-soluble dietary fiber content is 80.8 mass %,

(e) ratio of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is 1:2.2

(f) the total of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is full glucose residue 72.9%,

(g) the average glucose degree of polymerization is that 31, Mw/Mn is 2.0.

The active ingredient that this product can raise inhibitor as blood glucose value utilizes.In addition, by absorbing this product, it can suppress people's The rise of blood glucose value after monosaccharide intake.This product is usually to be grown up (weight 60kg) every 1 time, the scope of about 0.5~about 100g, This product is direct, alternatively, being dissolved in the beverage of water, tea, coffee etc. and absorbing, or it is added in Foods or drinks and absorbs i.e. Can.Certainly also can be by this product intake before and after the intake of Foods or drinks.This product itself is tasteless, no foreign odor, in room Also non-hygroscopic under temperature, discoloration, stablized through more than 1 year.

【Embodiment 2】

According to the method described in the experiment 2-2 of International Publication No. WO2008/136331 separate editions, solid constituent is modulated Branch's alpha-glucans mixture solution of 30 mass % of concentration, thereafter, is spray-dried and obtains branched alpha-Portugal according to conventional methods Oligosaccharide mixture powder.Furthermore obtained branch's alpha-glucans mix powder has the feature of following (a) or even (g).

(a) using glucose as being formed sugar,

(c) digested by isomaltose glucanase, isomaltose generated with the 27.2 mass % of every solids of digest,

(d) water-soluble dietary fiber content is 41.8 mass %,

(e) ratio of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is 1:0.6,

(f) the total of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is full glucose residue 83.0%,

(g) the average glucose degree of polymerization is that 405, Mw/Mn is 16.2.

【Embodiment 3】

According to the method described in the embodiment 6 of International Publication No. WO2008/136331 separate editions, branched alpha-Portugal is modulated Oligosaccharide mixture powder.Furthermore obtained branch's alpha-glucans mix powder has the feature of (a) or even (g).

(a) using glucose as being formed sugar,

(c) digested by isomaltose glucanase, isomaltose generated with the 40.6 mass % of every solids of digest,

(d) water-soluble dietary fiber content is 77.0 mass %,

(e) ratio of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is 1:4,

(f) the total of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is full glucose residue 67.9%,

(g) the average glucose degree of polymerization is that 18, Mw/Mn is 2.0.

【Embodiment 4】

In addition to 2 unit maltotetraoses generation amylase is added per 1g solids again to cornstarch liquefier, root According to the method described in the embodiment 5 of International Publication No. WO2008/136331 separate editions, modulation branch alpha-glucans mixture Powder.Furthermore obtained branch's alpha-glucans mix powder has the feature of (a) or even (g).

(a) using glucose as being formed sugar,

(c) digested by isomaltose glucanase, isomaltose generated with the 41.9 mass % of every solids of digest,

(d) water-soluble dietary fiber content is 69.1 mass %,

(e) ratio of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is 1:2.4

(f) the total of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is full glucose residue 64.2%,

(g) the average glucose degree of polymerization is that 13, Mw/Mn is 2.0.

【Embodiment 5】

Make amyloglucosidase (glucoamylase) act on the branched alpha that is obtained with the method described in embodiment 1- Glucan mixture, undecomposed component is taken using gel permeation chromatography point.Thereafter, purify and be spray-dried according to conventional methods And modulate branch's alpha-glucans mix powder.Furthermore obtained branch's alpha-glucans mixture has the feature of (a) or even (g).

(a) using glucose as being formed sugar,

(c) digested by isomaltose glucanase, isomaltose generated with the 21 mass % of every solids of digest,

(d) water-soluble dietary fiber content is 94.4 mass %,

(e) ratio of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is 1:1.9

(f) the total of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is full glucose residue 64%,

(g) glucose polymerization degree is that 22, Mw/Mn is 1.7.

【Embodiment 6】

For 33 mass parts of orange fruit juice powder by spray drying manufacture, by what is obtained with the method described in embodiment 5 10 mass parts of branch's alpha-glucans mix powder, 20 mass parts of glucose, 20 mass parts of anhydrous crystalline maltitol, anhydrous lemon 0.65 mass parts of lemon acid, 0.1 mass parts of malic acid, 2-O- be alpha-glucose-based -0.2 mass parts of L-AA, citric acid soda The appropriate good mixing of 0.1 mass parts and powder perfume, crushing form micropowder, it are fed intake to fluidized bed pelletizer, As 40 DEG C of temperature of outgoing air, the branch's alpha-glucans powder that will be obtained with the method for embodiment 1 of spraying in right amount as adhesive End is dissolved in solution obtained from water, is granulated within 30 minutes, metering, packs and obtain product.This product is to contain fruit juice containing monosaccharide The powder juice for having rate about 30%.This product is due to coordinate branch's alpha-glucans as the blood glucose value rise inhibitor containing monosaccharide Mixture, the elevated powder juice of blood glucose value when can inhibit intake.In addition, this product is free from extraneous odour, foreign odor, as juice commodity valency Value is high.

【Embodiment 7】

By 100 mass parts of cornstarch, the branch's alpha-glucans mix powder obtained with the method described in embodiment 4 30 mass parts, 70 mass parts of water crystallization containing trehalose, 40 mass parts of glucose and 1 mass parts of salt are sufficiently mixed, and add chicken 280 mass parts of egg and stir, slowly add the cow's milk 1 of boiling to it, 000 mass parts, then the roasting lasting stirring of fire, in cornstarch Fully be gelatinized and it is overall become translucent when only fire, it is cooled down, adds suitable chinese cymbidium spices, metering, filling, bag Fill and obtain product.This product is that the custard containing monosaccharide, disaccharides, polysaccharide is white.This product is due to as containing monosaccharide, two Carbohydrate, the blood glucose value rise inhibitor of polysaccharide coordinate branch alpha-glucans mixture, the liter of blood glucose value when can inhibit intake High custard frost.In addition, this product is that have smooth gloss, the custard frost of excellent flavor and high-quality.

【Embodiment 8】

With glucose 247g, the branch alpha-glucans mix powder 217g obtained used in the method described in embodiment 3, Mixture 15g, ascorbic acid before ferric pyrophosphate suspension (sun Chemical Corporation trade name SunActiveFeM) 8g, vitamin Sodium 3g, Zn-contained yeast 0.5g, chromium yeast 0.3g, sucralose 0.2g put into prilling granulator as former end is granulated.On the one hand, exist It is granulated adjustment dissolving coffee extract powder 15g, magnesium sulfate 10g in water 100mL.Make when granulation original is last to be mixed in the device from spray Little by little mist projection granulating adjusts liquid and granulates for the tip of mouth, and nitrogen is filled into aluminium bag to as 1 packaging 5.15g or 1 packaging 10.3g.This product is the dietary supplement containing monosaccharide.This product is due to match somebody with somebody as the blood glucose value rise inhibitor containing monosaccharide Branch alpha-glucans mixture is closed, the elevated dietary supplement of blood glucose value when can inhibit intake.In addition, this product is to be no different Taste, foreign odor are high as dietary supplement commodity value.

【Embodiment 9】

Manufacture using the branch's alpha-glucans mix powder obtained used in the method described in embodiment 1 and be used as this hair The black tea of bright oral composition.Relative to tealeaves 15g plus boiled water 1L, filtering tea and obtain black tea extracting solution 1L.With Add 60g isomerized sugars again to 1L black tea extracting solutions with the black tea of the addition of weight ratio 2%, 3%, 4% branch alpha-glucans mixture As respective black tea drinks A, B, C of the invention.In addition, in addition to being not added with the point of branch's alpha-glucans mixture, to use The black tea drinks that method similar to the above obtains are as control.When carrying out sensory evaluation by 20~men and women 10 over fifty years old, Learn the effect for covering the distinctive bitter taste of polyphenol contained in black tea drinks or astringent taste.Furthermore learn, black tea of the invention Even if beverage A, B, C are stored at room temperature, also with compare and bloom phenomenon (by the phenomenon of black tea slowly cold then gonorrhoea) It is suppressed.

This product is the black tea drinks containing monosaccharide.This product is due to coordinate as the blood glucose value rise inhibitor containing monosaccharide The elevated black tea drinks of branch's alpha-glucans mixture and blood glucose value when can inhibit intake.In addition, this product be free from extraneous odour, it is different It is smelly, it is high as black tea drinks commodity value.【Industrial applicibility】

It is as described above, suppression is raised by the blood glucose value of the invention using this branch alpha-glucans mixture as active ingredient Preparation, since itself low sweet taste of this branch alpha-glucans mixture as active ingredient is to tasteless, can obtain utilization scope The elevated benefit of blood glucose value that is wide, being not limited to after " people of diabetes borderline region " suppression monosaccharide intake.It is in addition, of the invention Oral composition due to monosaccharide together containing the present invention blood glucose value raise inhibitor, have also unnecessary even if being absorbed The elevated benefit of care about blood glucose value.The present invention makes this area huge contribution, meaningful invention really.

【The explanation of symbol】

In Fig. 1,

○：The average value (Δ blood glucose) of the increment of blood glucose value during the control sample that intake is only made of glucose

●：Intake and the increment of blood glucose value during test specimens of the glucose together containing branch's alpha-glucans mixture Average value (Δ blood glucose)

In fig. 2,

a：Rubber bolt

b：Exhaust aperture

c：Cut off the plastics 1mL chips of tip

d：Plastics 20mL holds injection tube

e：Invert small intestine

f：Serosa side buffer solution

g：Mucous membrane side buffer solution

h：95%O₂- 5%CO₂Gas backstreaming is managed

Claims

1. suppress people monosaccharide intake after blood glucose value elevated blood glucose value rise inhibitor, its with have following (A) or even (C) branch's alpha-glucans mixture of feature is as active ingredient：

(A) using glucose as being formed sugar,

(B) have through the key connection beyond α -1,4 keys to the straight-chain positioned at the glucose polymerization degree more than 3 through α -1,4 key connections The branched structure of the glucose polymerization degree more than 1 of the non-reducing end glucose residue of one end of glucan,

(C) generation isomaltose is digested by isomaltose glucanase.

2. the blood glucose value rise inhibitor described in claim 1, it is characterised in that above-mentioned branch's alpha-glucans mixture is by different Maltose dextranase digests, and isomaltose is generated with below the 70 mass % of more than 5 mass % of every solids of digest Branch's alpha-glucans mixture.

3. described in claim 1 or 2 blood glucose value rise inhibitor, wherein above-mentioned branch's alpha-glucans mixture be have it is following (D) branch's alpha-glucans mixture of feature：

(D) the water-soluble dietary fiber content obtained by high-performance liquid chromatograph method (enzyme-HPLC methods) is more than 40 mass %.

4. any one of them blood glucose value rise inhibitor of claims 1 to 3, wherein above-mentioned branch's alpha-glucans mixture is There is branch's alpha-glucans mixture of the feature of following (E) and (F)：

(E) ratio of the glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys is in 1:0.6~1:4 scope；And

(F) glucose residue of the glucose residue of α -1,4 keys and α -1,6 keys it is total account for the 55% of full glucose residue with On.

5. any one of them blood glucose value rise inhibitor of Claims 1 to 4, it is characterised in that above-mentioned branch's alpha-glucans The average glucose degree of polymerization of mixture is 8~500.

6. oral composition, it is characterised in that containing Claims 1 to 5 any one of them blood glucose value rise inhibitor and Monosaccharide.

7. the oral composition described in claim 6, it is characterised in that monosaccharide is at least one kind of selected from glucose and fructose.