CN108003202A - A kind of method that Sialic acid is extracted from bird's nest - Google Patents
A kind of method that Sialic acid is extracted from bird's nest Download PDFInfo
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- CN108003202A CN108003202A CN201711210022.0A CN201711210022A CN108003202A CN 108003202 A CN108003202 A CN 108003202A CN 201711210022 A CN201711210022 A CN 201711210022A CN 108003202 A CN108003202 A CN 108003202A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H13/00—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids
- C07H13/02—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids
- C07H13/04—Compounds containing saccharide radicals esterified by carbonic acid or derivatives thereof, or by organic acids, e.g. phosphonic acids by carboxylic acids having the esterifying carboxyl radicals attached to acyclic carbon atoms
- C07H13/06—Fatty acids
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
- C07H1/08—Separation; Purification from natural products
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Abstract
The invention belongs to bird's nest product deep process technology field, more particularly to a kind of method that Sialic acid is extracted from bird's nest.Bird's nest is first crushed to by 80 mesh by the method for the present invention, 3 4h are soaked with 50 60 DEG C of hot deionized waters, make the abundant water swelling of bird's nest powder, starched with colloid mill circular grinding by the bird's nest of 120 mesh, with microwave function by bird's nest breaking-wall cell, Sialic acid is set to be discharged from glycoprotein and mucus protein, the characteristics of foundation Sialic acid molecular weight is small, macromolecular substances are removed with the micro-filtration of membrane technology and 2 grades of ultrafiltration, the material of 150 500Da is retained with NF membrane again, by the trapped fluid of 150 500Da it is concentrated, freezing, microwave vacuum be dried under reduced pressure, obtain Sialic acid.For the bird's nest acid content that the method for the present invention obtains more than 80%, the rate of recovery of Sialic acid reaches 90%.
Description
Technical field
The invention belongs to bird's nest product deep process technology field, more particularly to a kind of side that Sialic acid is extracted from bird's nest
Method.
Background technology
Traditional Chinese medicine thinks：Bird's nest, it is sweet in flavor, it is mild-natured, it is nontoxic.Enter lung, stomach, kidney three to pass through.Function：Nourishing yin to moisten dryness, invigorating middle warmer and replenishing QI,
Preventing phlegm from forming and stopping coughing.Cure mainly：Prolonged illness is deficient, pulmonary tuberculosis cough, phlegm asthma, spits blood, and spits blood, protracted dysentery, chronic malaria, dysphagia and regurgitation, and weak seminal emission is small
Just frequency etc..Medication is avoided：Warm phlegm is stagnated and has the careful clothes of table heresy person.Therefore, for a long time, bird's nest is as gourmet food and bear's paw
Shark's fin is eponymous and makees strong invigorant and cosmetics and takes.Bird's nest is used as medicine starts from Ming Dynasty's middle and later periods according to textual criticism, away from the present existing 500
For many years.
It is reported that modern pharmacological research, bird's nest not only has profuse nutritional ingredient, also containing multiple biological activities thing
Matter, such as Sialic acid, bird's nest cell division hormone, epidermal growth factor, these biological active matter confrontation human bodies have weight
The biology adjustment effect wanted, but traditional eating method is pressed, these bioactive substances in bird's nest and the sugared egg in bird's nest
White and mucoprotein is combined closely, and is difficult to degrade and absorbed in vivo, the physiological action effect that they have cannot give full play to
Effect.In addition, these bioactive substances, it is being subject to acid, alkali, temperature, the enzyme can be by certain when factor is in subcritical range
Destruction and reduce its activity, or even lose activity.Therefore, compared with being isolated under temperate condition after extraction using can maximum journey
Its activity of degree protection.
In the application study of bird's nest, it is Sialic acid that one of achievement more and that acquirement is more is handed in research, and Sialic acid is called saliva
Liquid acid (sialic acid) is 9 carbon monosaccharide derivatives, its molecular formula is C11H19NO9.Relative molecular weight is 309.2689, knot
Structure formula is shown in Fig. 1.
Sialic acid is N or O acyl neuraminic acid, and neuraminic acid is, people sugared by 9 carbon of epichitosamine and acetone derivation
The sialic acid of body is N-acetyl-neuraminate (NeuAc), and N-acetyl-neuraminate is the composition portion of lipoprotein and gangliosides
Point, be naturally occurring carbohydrate, usually exist with oligosaccharide, glycolipid or glycoprotein forms, the sialic acid in bird's nest with
The form of glycoprotein and mucoprotein exists.Sialic acid is separated from the salivary gland mucoprotein of animal, so
The name of saliva, but found that the sialic acid that contains was all more than any material in bird's nest later, up to the 15% of bird's nest dry product, therefore
This, also known as Sialic acid in most cases.
Show according to the research both at home and abroad to Sialic acid, its physiological regulatory action is many, is also being furtherd investigate now
In, obtained highly recognition has following main function：
1) intellectual development of infant is improved, improves adult man memory and brain response ability.
2) absorbability of the enteron aisle to various nutritional ingredients is improved.
3) promote enteron aisle antibacterial toxin expelling, prevent pathologic coli-infection, adjust the half-life period of blood protein.
4) immunocompetence of body, particularly children are improved.
5) there are protection and stabilization to body cell, so as to reach the effect of promoting longevity.
It is reported that the research of sialic acid starts from American scientist BLIX in nineteen fifty-seven, divide first from the glandula mucus of ox
Sialic acid is separated out, and system research is carried out to its chemical property, molecular structure, biosynthesis etc., finds it with many important
Biological function.Hereafter, many scientists follow this further investigation, obtain many achievements.
The research that Sialic acid influences brain is relatively abundant, and numerous studies find that the content of mankind's brain cell film Sialic acid is
2-4 times of other animal brain cell membranes, is 15 times of organ such as liver, lung etc. in same body.Australian scholar's research
It was found that the Sialic acid in the colostrum of breast milk reaches 5.04 ± 0.21 mM/ls of concentration, hereafter gradually decrease to baby and go out
Decline about 80% after 3 months raw, its concentration only has the 1/5 of colostrum.In economically developed country, the child of dairy baby food is eaten
Son, it is fewer than the child with breast-feeding absorb at least 20% Sialic acid.One group of scientist of New Zealand is verification Sialic acid to intelligence
The influence of power development, to 1025 tested children, the follow-up study graduated from the high school from birth by 18 years old, the results showed that：Breast milk is fed
Foster baby, tests in IQ testing standards, and the aspects such as the general performance and fraction in evaluation of teacher and middle school's period is superior to use
Dairy products fed infant, the reason is that the Sialic acid of the latter's intake is than with few the 20% of breast-feeding.The time of breast-feeding
Longer, the integrated intelligence development level of children is higher.Sialic acid is that Sialic acid passes through brain cell film to the Influencing Mechanism of brain
Yu the interaction of Tu Touch, the reaction speed of brain nervous cell Tu Touch is improved, so as to improve memory and reaction fast.
The method that Sialic acid is extracted from bird's nest, disclosed document has not seen, is briefly mentioned also few.It is reported that Chen Ling
The bird's nest containing Sialic acid 13.4% is digested Deng with papain, is 1 in solid-to-liquid ratio：30, pH value 8.0, temperature is 45 DEG C,
Under conditions of when enzymolysis time 2.4 is small, the recovery rate of Sialic acid is 9.98%, higher by 9.91% than with water extraction.Martin reports,
Carried by water, 3 steps such as ultrafiltration and ion exchange, the Sialic acid that purity reaches gram level can be obtained.
The information for being disclosed in the background section is merely intended to understanding of the increase to the general background of the present invention, without answering
It has been the prior art well known to persons skilled in the art when being considered as recognizing or implying the information structure in any form.
The content of the invention
For overcome the deficiencies in the prior art, the present invention provides a kind of method that Sialic acid is extracted from bird's nest, and this method is led to
Cross fine grinding, microwave frequency measurment, membrane technology purification ＆ isolation, microwave drying obtain product, after testing, the Sialic acid of gained bird's nest acid product
Content is up to more than 80%, and bird's nest acid recovering rate is up to more than 90%.
Technical solution provided by the invention is as follows：
A kind of method that Sialic acid is extracted from bird's nest, includes the following steps：
(1) bird's nest is first dried into 2-3h in electric dry oven, bird's nest dehydration is become fragile；
(2) bird's nest after drying is crushed with pulverizer again, obtains bird's nest powder；
(3) bird's nest powder 3-4h is soaked with deionized water, makes to fully absorb moisture and be expanded to 5-6 times, into translucent micelle shape；
(4) soaked bird's nest powder is ground together with soaking water with colloid mill, circular grinding 8-10min, is ground into 120 purposes
Bird's nest is starched, and addition deionized water makes solid-to-liquid ratio up to 1：30, then with dilute NaOH solution adjusting slurry pH value to 10.6, ground by colloid mill
Mix；
(5) slurries that will be mixed up, are placed in the microwave tank that microwave frequency is 2450MHz, 60 DEG C of temperature control, microwave treatment time
25-30min, after microwave treatment, 50min is kept the temperature at 50-60 DEG C；Material liquid pH value is detected, it is cold etc. feed liquid as pH value ＞ 8.4
But extremely<At 45 DEG C, then microwave treatment 15min, keep the temperature 15min；Work as pH value<When 8.4, terminate microwave treatment process, when discharge is expected
Liquid cools down<At 45 DEG C, with the pH value of acetic acid flavouring liquid to 6.5-7.0；
(6) the bird's nest slurries of adjusting slurry pH value to 6.5-7.0 are first subjected to micro-filtration with hollow-fibre membrane, dope is collected and treated
With permeate uses ultrafiltration membrance filter again；
(7) micro-filtration permeate 10000Da and 20000Da two-stage ultrafiltration, 10000Da control pump pressure 0.1-0.2Mpa, film
Surface current amount 1-1.5m/s, cycles of concentration 4-5；
(8) 20000Da ultrafiltration permeate is filtered with two-stage nanofiltration, the 1st grade with 500Da, the 2nd grade with 200Da, operating parameter
It is pumped 0.6-0.8MPa, circular flow 1.5-2m3/ h, cycles of concentration 6-8；
(9) 500Da permeate and 200Da are intercepted into liquid through being concentrated under reduced pressure into Be25 degree, after discharge is cooled to room temperature, are placed in
In the refrigerator-freezer or freezer of energy cryogenic refrigeration, -37 DEG C are refrigerated to, and keep 1-2h；
(10) -37 DEG C will be refrigerated to and keep the ice cube of 1-2h to take out, fritter is broken into, is placed in microwave vacuum dryer
Dehydration and drying, when drying low to product moisture content 6%, terminates drying, obtains the bird's nest acid product of loose shape；
(11) again by micro-filtration dope, the dope of 2 grades of ultrafiltration and the dope of 500Da nanofiltrations merge, then by above-mentioned steps (9) and
(10) operating method in, obtained bird's nest powder product.
Preferably, the temperature of electric dry oven is 50 DEG C in step (1).
Preferably, the fineness of bird's nest powder is 80 mesh in step (2).
Preferably, the temperature of deionized water is 50-60 DEG C in step (3).
Preferably, the vacuum of microwave vacuum dryer is 0.04-0.08MPa in step (10), temperature is 58 DEG C.
Preferably, the cryogenic temperature of concentrate is -35 DEG C in step (11).
Compared with prior art, the present invention has the advantages that：
(1) method of the invention obtains product, gained by fine grinding, microwave frequency measurment, membrane technology purification ＆ isolation, microwave drying
Product bird's nest acid content is up to more than 80%, and bird's nest acid recovering rate is up to more than 90%.
(2) method technique of the invention is simple, and equipment is simple, easily operated control.
Brief description of the drawings
Fig. 1 is the molecular structural formula of Sialic acid；
Fig. 2 is the process flow chart that Sialic acid is extracted in the slave bird's nest of the present invention.
Embodiment
Following embodiments do not limit the present invention to be better understood from the present invention.Experimental method in following embodiments,
Unless otherwise specified, it is conventional method.
Embodiment 1：
A kind of method that Sialic acid is extracted from bird's nest, includes the following steps：
(1) bird's nest is first dried into 2h in electric dry oven, bird's nest dehydration is become fragile；The temperature of the electric dry oven is 50 DEG C；
(2) bird's nest after drying is crushed with pulverizer again, obtains bird's nest powder；The fineness of the bird's nest powder is 80 mesh；
(3) bird's nest powder 3h is soaked with deionized water, makes to fully absorb moisture and be expanded to 5 times, into translucent micelle shape；It is described
The temperature of deionized water is 50 DEG C；
(4) soaked bird's nest powder is ground together with soaking water with colloid mill, circular grinding 8min, is ground into the swallows of 120 mesh
Lodgment of stock, addition deionized water make solid-to-liquid ratio up to 1：30, then with dilute NaOH solution adjusting slurry pH value to 10.6, ground by colloid mill mixed
It is even；
(5) slurries that will be mixed up, are placed in the microwave tank that microwave frequency is 2450MHz, 60 DEG C of temperature control, microwave treatment time
25min, after microwave treatment, 50min is kept the temperature at 50 DEG C；Material liquid pH value is detected, as pH value ＞ 8.4, is cooled to etc. feed liquid<45
DEG C when, then microwave treatment 15min, keeps the temperature 15min；Work as pH value<When 8.4, terminate microwave treatment process, when discharge feed liquid cooling<
At 45 DEG C, with the pH value of acetic acid flavouring liquid to 6.5；
(6) the bird's nest slurries of adjusting slurry pH value to 6.5 are first subjected to micro-filtration with hollow-fibre membrane, dope collection is stand-by, thoroughly
Cross liquid and use ultrafiltration membrance filter again；
(7) micro-filtration permeate 10000Da and 20000Da two-stage ultrafiltration, 10000Da control pump pressure 0.1Mpa, film surface stream
Measure 1m/s, cycles of concentration 4；
(8) 20000Da ultrafiltration permeate is filtered with two-stage nanofiltration, the 1st grade with 500Da, the 2nd grade with 200Da, operating parameter
It is pumped 0.6MPa, circular flow 1.5m3/ h, cycles of concentration 6；
(9) 500Da permeate and 200Da are intercepted into liquid through being concentrated under reduced pressure into Be25 degree, after discharge is cooled to room temperature, are placed in
In the refrigerator-freezer or freezer of energy cryogenic refrigeration, -37 DEG C are refrigerated to, and keep 1h；
(10) -37 DEG C will be refrigerated to and keep the ice cube of 1h to take out, be broken into fritter, be placed in microwave vacuum dryer and take off
Water is dried, and when drying low to product moisture content 6%, is terminated drying, is obtained the bird's nest acid product of loose shape；The ripple vacuum desiccator
Vacuum be 0.04MPa, temperature is 58 DEG C；
(11) again by micro-filtration dope, the dope of 2 grades of ultrafiltration and the dope of 500Da nanofiltrations merge, then by above-mentioned steps (9) and
(10) operating method in, obtained bird's nest powder product；The cryogenic temperature of concentrate is -35 DEG C.
Embodiment 2：
A kind of method that Sialic acid is extracted from bird's nest, includes the following steps：
(1) bird's nest is first dried into 3h in electric dry oven, bird's nest dehydration is become fragile；The temperature of the electric dry oven is 50 DEG C；
(2) bird's nest after drying is crushed with pulverizer again, obtains bird's nest powder；The fineness of the bird's nest powder is 80 mesh；
(3) bird's nest powder 4h is soaked with deionized water, makes to fully absorb moisture and be expanded to 6 times, into translucent micelle shape；It is described
The temperature of deionized water is 60 DEG C；
(4) soaked bird's nest powder is ground together with soaking water with colloid mill, circular grinding 10min, is ground into the swallows of 120 mesh
Lodgment of stock, addition deionized water make solid-to-liquid ratio up to 1：30, then with dilute NaOH solution adjusting slurry pH value to 10.6, ground by colloid mill mixed
It is even；
(5) slurries that will be mixed up, are placed in the microwave tank that microwave frequency is 2450MHz, 60 DEG C of temperature control, microwave treatment time
30min, after microwave treatment, 50min is kept the temperature at 60 DEG C；Material liquid pH value is detected, as pH value ＞ 8.4, is cooled to etc. feed liquid<45
DEG C when, then microwave treatment 15min, keeps the temperature 15min；Work as pH value<When 8.4, terminate microwave treatment process, when discharge feed liquid cooling<
At 45 DEG C, with the pH value of acetic acid flavouring liquid to 7.0；
(6) the bird's nest slurries of adjusting slurry pH value to 7.0 are first subjected to micro-filtration with hollow-fibre membrane, dope collection is stand-by, thoroughly
Cross liquid and use ultrafiltration membrance filter again；
(7) micro-filtration permeate 10000Da and 20000Da two-stage ultrafiltration, 10000Da control pump pressure 0.2Mpa, film surface stream
Measure 1.5m/s, cycles of concentration 5；
(8) 20000Da ultrafiltration permeate is filtered with two-stage nanofiltration, the 1st grade with 500Da, the 2nd grade with 200Da, operating parameter
It is pumped 0.8MPa, circular flow 2m3/ h, cycles of concentration 8；
(9) 500Da permeate and 200Da are intercepted into liquid through being concentrated under reduced pressure into Be25 degree, after discharge is cooled to room temperature, are placed in
In the refrigerator-freezer or freezer of energy cryogenic refrigeration, -37 DEG C are refrigerated to, and keep 2h；
(10) -37 DEG C will be refrigerated to and keep the ice cube of 2h to take out, be broken into fritter, be placed in microwave vacuum dryer and take off
Water is dried, and when drying low to product moisture content 6%, is terminated drying, is obtained the bird's nest acid product of loose shape；The ripple vacuum desiccator
Vacuum be 0.08MPa, temperature is 58 DEG C；
(11) again by micro-filtration dope, the dope of 2 grades of ultrafiltration and the dope of 500Da nanofiltrations merge, then by above-mentioned steps (9) and
(10) operating method in, obtained bird's nest powder product；The cryogenic temperature of concentrate is -35 DEG C.
Embodiment 3：
A kind of method that Sialic acid is extracted from bird's nest, includes the following steps：
(1) bird's nest is first dried into 2.5h in electric dry oven, bird's nest dehydration is become fragile；The temperature of the electric dry oven is 50
℃；
(2) bird's nest after drying is crushed with pulverizer again, obtains bird's nest powder；The fineness of the bird's nest powder is 80 mesh；
(3) bird's nest powder 3.5h is soaked with deionized water, makes to fully absorb moisture and be expanded to 5 times, into translucent micelle shape；Institute
The temperature for stating deionized water is 55 DEG C；
(4) soaked bird's nest powder is ground together with soaking water with colloid mill, circular grinding 9min, is ground into the swallows of 120 mesh
Lodgment of stock, addition deionized water make solid-to-liquid ratio up to 1：30, then with dilute NaOH solution adjusting slurry pH value to 10.6, ground by colloid mill mixed
It is even；
(5) slurries that will be mixed up, are placed in the microwave tank that microwave frequency is 2450MHz, 60 DEG C of temperature control, microwave treatment time
28min, after microwave treatment, 50min is kept the temperature at 55 DEG C；Material liquid pH value is detected, as pH value ＞ 8.4, is cooled to etc. feed liquid<45
DEG C when, then microwave treatment 15min, keeps the temperature 15min；Work as pH value<When 8.4, terminate microwave treatment process, when discharge feed liquid cooling<
At 45 DEG C, with the pH value of acetic acid flavouring liquid to 6.8；
(6) the bird's nest slurries of adjusting slurry pH value to 6.8 are first subjected to micro-filtration with hollow-fibre membrane, dope collection is stand-by, thoroughly
Cross liquid and use ultrafiltration membrance filter again；
(7) micro-filtration permeate 10000Da and 20000Da two-stage ultrafiltration, 10000Da control pump pressure 0.15Mpa, film surface stream
Measure 1.2m/s, cycles of concentration 4.5；
(8) 20000Da ultrafiltration permeate is filtered with two-stage nanofiltration, the 1st grade with 500Da, the 2nd grade with 200Da, operating parameter
It is pumped 0.7MPa, circular flow 1.8m3/ h, cycles of concentration 7；
(9) 500Da permeate and 200Da are intercepted into liquid through being concentrated under reduced pressure into Be25 degree, after discharge is cooled to room temperature, are placed in
In the refrigerator-freezer or freezer of energy cryogenic refrigeration, -37 DEG C are refrigerated to, and keep 1.5h；
(10) -37 DEG C will be refrigerated to and keep the ice cube of 1.5h to take out, fritter is broken into, is placed in microwave vacuum dryer
Dehydration and drying, when drying low to product moisture content 6%, terminates drying, obtains the bird's nest acid product of loose shape；The ripple vacuum drying
The vacuum of device is 0.06MPa, and temperature is 58 DEG C；
(11) again by micro-filtration dope, the dope of 2 grades of ultrafiltration and the dope of 500Da nanofiltrations merge, then by above-mentioned steps (9) and
(10) operating method in, obtained bird's nest powder product；The cryogenic temperature of concentrate is -35 DEG C.
The bird's nest acid product and bird's nest acid recovering rate that are obtained using method in embodiment 1-3 are measured, the results are shown in Table
1。
The testing result of bird's nest acid product is prepared in the method for the present invention of table 1
| Group | Bird's nest acid content (%) | Bird's nest acid recovering rate (%) |
| Embodiment 1 | 83 | 95 |
| Embodiment 2 | 82 | 96 |
| Embodiment 3 | 85 | 98 |
As shown in Table 1, the product that method of the invention is prepared contains Sialic acid more than 80%, the rate of recovery of Sialic acid
Reach 90%.
It is foregoing to the present invention specific exemplary embodiment description be in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to the above instruction, can be much changed
And change.The purpose of selecting and describing the exemplary embodiment is that explain that the certain principles of the present invention and its reality should
With so that those skilled in the art can realize and utilize the present invention a variety of exemplaries and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (6)
- A kind of 1. method that Sialic acid is extracted from bird's nest, it is characterised in that include the following steps：(1) bird's nest is first dried into 2-3h in electric dry oven, bird's nest dehydration is become fragile；(2) bird's nest after drying is crushed with pulverizer again, obtains bird's nest powder；(3) bird's nest powder 3-4h is soaked with deionized water, makes to fully absorb moisture and be expanded to 5-6 times, into translucent micelle shape；(4) soaked bird's nest powder is ground together with soaking water with colloid mill, circular grinding 8-10min, is ground into the bird's nests of 120 mesh Slurry, addition deionized water make solid-to-liquid ratio up to 1：30, then with dilute NaOH solution adjusting slurry pH value to 10.6, ground by colloid mill mixed It is even；(5) slurries that will be mixed up, are placed in the microwave tank that microwave frequency is 2450MHz, 60 DEG C of temperature control, microwave treatment time 25- 30min, after microwave treatment, 50min is kept the temperature at 50-60 DEG C；Material liquid pH value is detected, as pH value ＞ 8.4, is cooled to etc. feed liquid <At 45 DEG C, then microwave treatment 15min, keep the temperature 15min；Work as pH value<When 8.4, terminate microwave treatment process, when discharge feed liquid is cold But<At 45 DEG C, with the pH value of acetic acid flavouring liquid to 6.5-7.0；(6) the bird's nest slurries of adjusting slurry pH value to 6.5-7.0 are first subjected to micro-filtration with hollow-fibre membrane, dope collection is stand-by, thoroughly Cross liquid and use ultrafiltration membrance filter again；(7) micro-filtration permeate 10000Da and 20000Da two-stage ultrafiltration, 10000Da control pump pressure 0.1-0.2Mpa, film surface stream Measure 1-1.5m/s, cycles of concentration 4-5；(8) 20000Da ultrafiltration permeate is filtered with two-stage nanofiltration, the 1st grade with 500Da, the 2nd grade with 200Da, operating parameter pump pressure 0.6-0.8MPa, circular flow 1.5-2m3/ h, cycles of concentration 6-8；(9) 500Da permeate and 200Da are intercepted into liquid through being concentrated under reduced pressure into Be25 degree, after discharge is cooled to room temperature, being placed in can be deep Spend in the refrigerator-freezer or freezer of freezing, be refrigerated to -37 DEG C, and keep 1-2h；(10) -37 DEG C will be refrigerated to and keep the ice cube of 1-2h to take out, fritter is broken into, is placed in microwave vacuum dryer and is dehydrated Dry, when drying low to product moisture content 6%, terminate drying, obtain the bird's nest acid product of loose shape；(11) again by micro-filtration dope, the dope of 2 grades of ultrafiltration and the dope of 500Da nanofiltrations merge, then by above-mentioned steps (9) and (10) Middle operating method, obtained bird's nest powder product.
- 2. the method according to claim 1 that Sialic acid is extracted from bird's nest, it is characterised in that electric dry oven in step (1) Temperature be 50 DEG C.
- 3. the method according to claim 1 that Sialic acid is extracted from bird's nest, it is characterised in that bird's nest powder in step (2) Fineness be 80 mesh.
- 4. the method according to claim 1 that Sialic acid is extracted from bird's nest, it is characterised in that deionization in step (3) The temperature of water is 50-60 DEG C.
- 5. the method according to claim 1 that Sialic acid is extracted from bird's nest, it is characterised in that microwave is true in step (10) The vacuum of empty drier is 0.04-0.08MPa, and temperature is 58 DEG C.
- 6. the method according to claim 1 that Sialic acid is extracted from bird's nest, it is characterised in that concentrate in step (11) Cryogenic temperature be -35 DEG C.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902941A (en) * | 2018-06-28 | 2018-11-30 | 燕生（福建）生物工程有限公司 | A method of extracting Sialic acid from bird's nest |
| CN108936612A (en) * | 2018-06-28 | 2018-12-07 | 燕生（福建）生物工程有限公司 | A method of preparing the bird's nest extracting solution with strong antioxidant action |
| CN108970167A (en) * | 2018-06-29 | 2018-12-11 | 燕生（福建）生物工程有限公司 | A kind of extract equipment of Sialic acid |
| CN108997450A (en) * | 2018-06-28 | 2018-12-14 | 燕生（福建）生物工程有限公司 | A kind of method that crystallization mode extracts Sialic acid from bird's nest |
| CN109770163A (en) * | 2019-01-07 | 2019-05-21 | 塔尔普(北京)制药技术有限公司 | A method of endogenous hormone in removal bird's nest albumen source |
| CN110140955A (en) * | 2019-06-20 | 2019-08-20 | 华东川峰(厦门)生物科技有限公司 | A method of it reducing sialic acid in bird's nest treatment process and is lost |
| CN110283219A (en) * | 2019-07-02 | 2019-09-27 | 嘉必优生物技术（武汉）股份有限公司 | A kind of preparation method of saliva acid crystal |
| KR20210131039A (en) * | 2020-04-23 | 2021-11-02 | 주식회사 케이제이엠바이오 | Composition of Nutrient Delivery System for Improving Brain Function |
| CN114891051A (en) * | 2022-06-21 | 2022-08-12 | 小仙炖霸州食品有限公司 | Method for extracting cubilose acid by ultrasonic wave combined ball milling crushing method |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902941A (en) * | 2018-06-28 | 2018-11-30 | 燕生（福建）生物工程有限公司 | A method of extracting Sialic acid from bird's nest |
| CN108936612A (en) * | 2018-06-28 | 2018-12-07 | 燕生（福建）生物工程有限公司 | A method of preparing the bird's nest extracting solution with strong antioxidant action |
| CN108997450A (en) * | 2018-06-28 | 2018-12-14 | 燕生（福建）生物工程有限公司 | A kind of method that crystallization mode extracts Sialic acid from bird's nest |
| CN108970167A (en) * | 2018-06-29 | 2018-12-11 | 燕生（福建）生物工程有限公司 | A kind of extract equipment of Sialic acid |
| CN109770163A (en) * | 2019-01-07 | 2019-05-21 | 塔尔普(北京)制药技术有限公司 | A method of endogenous hormone in removal bird's nest albumen source |
| CN110140955A (en) * | 2019-06-20 | 2019-08-20 | 华东川峰(厦门)生物科技有限公司 | A method of it reducing sialic acid in bird's nest treatment process and is lost |
| CN110283219A (en) * | 2019-07-02 | 2019-09-27 | 嘉必优生物技术（武汉）股份有限公司 | A kind of preparation method of saliva acid crystal |
| CN110283219B (en) * | 2019-07-02 | 2023-03-24 | 嘉必优生物技术（武汉）股份有限公司 | Preparation method of sialic acid crystal |
| KR20210131039A (en) * | 2020-04-23 | 2021-11-02 | 주식회사 케이제이엠바이오 | Composition of Nutrient Delivery System for Improving Brain Function |
| KR102439576B1 (en) * | 2020-04-23 | 2022-09-02 | 주식회사 케이제이엠바이오 | Composition of Nutrient Delivery System for Improving Brain Function |
| CN114891051A (en) * | 2022-06-21 | 2022-08-12 | 小仙炖霸州食品有限公司 | Method for extracting cubilose acid by ultrasonic wave combined ball milling crushing method |
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