CN107998167A - A kind of method that oxidation-resistant active ingredient is extracted from cauliflower - Google Patents
A kind of method that oxidation-resistant active ingredient is extracted from cauliflower Download PDFInfo
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- CN107998167A CN107998167A CN201711333462.5A CN201711333462A CN107998167A CN 107998167 A CN107998167 A CN 107998167A CN 201711333462 A CN201711333462 A CN 201711333462A CN 107998167 A CN107998167 A CN 107998167A
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- cauliflower
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- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 title claims abstract description 47
- 240000003259 Brassica oleracea var. botrytis Species 0.000 title claims abstract description 47
- 239000004480 active ingredient Substances 0.000 title claims abstract description 12
- 238000000034 method Methods 0.000 title claims abstract description 6
- 230000003647 oxidation Effects 0.000 title claims description 8
- 238000007254 oxidation reaction Methods 0.000 title claims description 8
- 230000003064 anti-oxidating effect Effects 0.000 claims abstract description 18
- 230000000694 effects Effects 0.000 claims abstract description 13
- 238000000926 separation method Methods 0.000 claims abstract description 10
- -1 hydroxyl radical free radical Chemical class 0.000 claims abstract description 8
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 8
- 239000001301 oxygen Substances 0.000 claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 42
- 239000000706 filtrate Substances 0.000 claims description 24
- 238000000108 ultra-filtration Methods 0.000 claims description 13
- 239000003963 antioxidant agent Substances 0.000 claims description 11
- 230000003078 antioxidant effect Effects 0.000 claims description 11
- 235000006708 antioxidants Nutrition 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 11
- 239000012528 membrane Substances 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 7
- 238000002137 ultrasound extraction Methods 0.000 claims description 7
- 238000002474 experimental method Methods 0.000 claims description 6
- 230000002000 scavenging effect Effects 0.000 claims description 5
- 238000001914 filtration Methods 0.000 claims description 4
- 238000012795 verification Methods 0.000 claims description 4
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 3
- 244000060011 Cocos nucifera Species 0.000 claims 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 abstract description 6
- 230000036541 health Effects 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- 239000003814 drug Substances 0.000 abstract description 4
- 241000196324 Embryophyta Species 0.000 abstract description 3
- 239000003960 organic solvent Substances 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract 1
- 239000000047 product Substances 0.000 description 8
- 150000003254 radicals Chemical class 0.000 description 7
- 238000003786 synthesis reaction Methods 0.000 description 4
- 235000013311 vegetables Nutrition 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 241000737241 Cocos Species 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 229930003935 flavonoid Natural products 0.000 description 2
- 150000002215 flavonoids Chemical class 0.000 description 2
- 235000017173 flavonoids Nutrition 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 206010006187 Breast cancer Diseases 0.000 description 1
- 208000026310 Breast neoplasm Diseases 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 238000001311 chemical methods and process Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000012459 cleaning agent Substances 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 235000012041 food component Nutrition 0.000 description 1
- 239000012458 free base Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000000638 solvent extraction Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 230000002087 whitening effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/31—Brassicaceae or Cruciferae (Mustard family), e.g. broccoli, cabbage or kohlrabi
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/17—Preparation or pretreatment of starting material involving drying, e.g. sun-drying or wilting
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The present invention provides a kind of method that the active ingredient with anti-oxidation efficacy is isolated from cauliflower.The cauliflower isolate prepared according to the method for the present invention has good antioxidation activity, when isolate mass concentration reaches 0.4mg/ml, can effectively remove DPPH, hydroxyl radical free radical and ultra-oxygen anion free radical, clearance rate reaches more than 70%.The isolate comes from natural plants, safe, is expected to be applied in the products such as medicine, health products, skin care item and other daily chemicals.Separation method provided by the present invention is simple and practicable, of low cost, and separation process is not related to harmful organic solvent, and non-environmental-pollution, is adapted to industrialized production.
Description
Technical field
The present invention relates to a kind of method of the extraction with oxidation-resistant active ingredient from cauliflower, belong to plant component extraction
Separation field.
Background technology
Cauliflower is annual herb plant, contains multivitamin and trace element, rich in nutrition content.Cauliflower is
Containing one of most food of flavonoids, flavonoids is except can prevent from infecting, or best blood vessel cleaning agent, can prevent
Cholesterol oxidation, prevents that coagulating platelets are blocking, thus reduces the danger of heart disease and apoplexy.Studied according to U.S. nutritionist,
There are a variety of indole derivativeses in cauliflower, this compound plays the role of reducing human body inner estrogen level, can prevent breast cancer
Generation.In addition also contain dithiol thioketones in cauliflower, the enzyme to form melanin can be reduced and prevent skin pigment spot
Formed, it is often edible lubricious to whet the appetite, there is good whitening effect to skin.
Cauliflower have the function of multiple nutritional components and, there are many benefits to human body, to the component of cauliflower into
Row analysis, and these advantageous components are converted into finished product and are widely used in the products such as food, medicine, health products, daily chemicals
In, the prevention of the daily disease of guiding people's progress will be efficiently contributed to and to own health by developing all multi-products of cauliflower
Safeguard.
Antioxidant is that one kind can help to capture and neutralize free radical, so as to dispel one kind that free radical damages human body
Material.The antioxidant of human body may be from itself synthesis, also can be by food supply.Scientific investigations showed that cancer, aging or its
Its disease is mostly relevant with the generation of excessive free radicals.Study it is anti-oxidant can effectively overcome harm caused by its, so
It is anti-oxidant to be classified as one of R＆D direction by medicine, health products, skin care item enterprise and other daily chemical enterprises, and market is most heavy
One of feature demand wanted.Antioxidant such as BHA, BHT due to the synthesis of conventionally employed chemical technology etc. have toxic side effect,
Thus its additive amount in the product is strictly controlled by various countries.The natural antioxidant come from animal vegetable tissue will be effective
Solve the problems, such as the toxic side effect of chemical synthesis antioxidant.
Cauliflower is one of important vegetable crop in China, and one of main foreign exchange earning vegetables in China, it is planted
Region almost covers all parts of the country, this causes cauliflower to derive from a wealth of sources, of low cost, and the utilization to its active ingredient will have
There is very vast market prospect.It is contemplated that found from this vegetables of low cost of cauliflower with antioxidation activity
Component, to substitute traditional chemical technique synthesis antioxidant.
The content of the invention
In order to extract the antioxidant content in cauliflower in a manner of simple, of low cost, safe and efficient, the present invention
Using ethanol as the active ingredient in solvent extraction cauliflower, and cauliflower component is carried out slightly by the way of centrifugal ultrafiltration
Separation, the antioxidation activity for the different component that the ultrafiltration membrane by studying different pore size size is retained down, finds in cauliflower
Component of the molecular weight less than 1KDa has very strong antioxidation activity.
Embodiment of the present invention is specific as follows.
Step 1：Cauliflower is taken, in 50 DEG C~60 DEG C of baking oven after dry 20h~24h, is carried out using micronizer
Crush.Powder after crushing continues in 50 DEG C~60 DEG C of baking oven and dries 7h~10h.
Step 2：Dried cauliflower powder ultrasound in 60%~75% ethanol is taken to extract 6h~8h, ethanol volume is
10~20 times of cauliflower quality, filtering；Filter residue is continued in 60%~75% ethanol and filtered after ultrasound extraction 6h~8h, continuously
3 times, merge 3 filtrates.
Step 3：Filtrate is carried out rotation to be evaporated, vapors away ethanol, until filtrate does not have alcohol taste.
Step 4：Filtered after step 3 gained filtrate is diluted one times using 0.22 μm of filter membrane, collect filtrate, filtrate is adopted
With 1KDa ultrafiltration membranes, in 1 DEG C~5 DEG C, centrifugal ultrafiltration collects the component that molecular weight is less than 1KDa under conditions of 3000g~5000g,
The component that antioxidation activity is most strong in cauliflower is obtained, after being freezed to the component, is configured to the solution of various concentrations gradient, is carried out
DPPH radicals scavengings are tested, and hydroxyl radical free radical removes experiment and its anti-oxidant work of ultra-oxygen anion free radical removing experimental verification
Property.
Preferably, the drying temperature of cauliflower is 60 DEG C in step 1 of the present invention, drying time 20h.To flower coconut palm
After dish carries out ultramicro grinding, the optimum temperature of powder drying is 60 DEG C, drying time 8h.
Preferably, the concentration of alcohol employed in step 2 of the present invention is 60%, and it is flower coconut palm to use ethanol volume
15 times of dish quality.
Preferably, the centrifugal force employed in step 4 of the present invention is 3000g.
Extract provided by the present invention to the clearance rate of DPPH, hydroxyl radical free radical and ultra-oxygen anion free radical with
The increase of extract concentrations and increase, when mass concentration reaches 0.4mg/ml, more than 70% can be reached to three's clearance rate,
Show good antioxidation activity.
A kind of oxidation-resistant active ingredient isolated from cauliflower provided by the invention is to DPPH free radicals, hydroxyl free
Base and ultra-oxygen anion free radical have good scavenging action, and separating technology is simple, easily operated, of low cost, separation
Process is not related to the use of harmful organic solvent, is suitably applied the exploitation and application of health products, medicine, daily chemical products.
Embodiment
The present invention is described in further detail with reference to embodiments.
Embodiment 1.
Step 1：Cauliflower is taken, in 60 DEG C of baking oven after dry 20h, is crushed using micronizer.After crushing
Powder continue at dry 8h in 60 DEG C of baking oven.
Step 2：Dried cauliflower powder ultrasound extraction 8h in 60% ethanol is taken, ethanol volume is cauliflower matter
15 times of amount, filtering；Filter residue is continued in 60% ethanol and filtered after ultrasound extraction 8h, continuous 3 times, merges 3 filtrates.
Step 3：Filtrate is carried out rotation to be evaporated, vapors away ethanol, until filtrate does not have alcohol taste.
Step 4：Filtered after step 3 gained filtrate is diluted one times using 0.22 μm of filter membrane, collect filtrate, filtrate is adopted
With 1KDa ultrafiltration membranes, in 4 DEG C, centrifugal ultrafiltration collects the component that molecular weight is less than 1KDa under conditions of 3000g, obtains in cauliflower
The most strong component of antioxidation activity, after being freezed to the component, is configured to the solution of various concentrations gradient, it is clear to carry out DPPH free radicals
Except experiment, hydroxyl radical free radical removes experiment and its antioxidation activity of ultra-oxygen anion free radical removing experimental verification, the result is shown under
Table.
Embodiment 2.
Step 1：Cauliflower is taken, in 50 DEG C of baking oven after dry 24h, is crushed using micronizer.After crushing
Powder continue at dry 10h in 50 DEG C of baking oven.
Step 2：Dried cauliflower powder ultrasound extraction 6h in 70% ethanol is taken, ethanol volume is cauliflower matter
20 times of amount, filtering；Filter residue is continued in 70% ethanol and filtered after ultrasound extraction 6h, continuous 3 times, merges 3 filtrates.
Step 3：Filtrate is carried out rotation to be evaporated, vapors away ethanol, until filtrate does not have alcohol taste.
Step 4：Filtered after step 3 gained filtrate is diluted one times using 0.22 μm of filter membrane, collect filtrate, filtrate is adopted
With 1KDa ultrafiltration membranes, in 2 DEG C, centrifugal ultrafiltration collects the component that molecular weight is less than 1KDa under conditions of 5000g, obtains in cauliflower
The most strong component of antioxidation activity, after being freezed to the component, is configured to the solution of various concentrations gradient, it is clear to carry out DPPH free radicals
Except experiment, hydroxyl radical free radical removes experiment and its antioxidation activity of ultra-oxygen anion free radical removing experimental verification, the result is shown under
Table.
The two embodiment results more than, the molecular weight extracted from cauliflower is less than the component of 1KDa, to DPPH, hydroxyl
Base free radical and ultra-oxygen anion free radical all have relatively good Scavenging activity, when extract quality concentration reaches 0.4mg/ml
When, more than 70% can be reached to three's Scavenging activity, and its oxidation resistance increases with the increase of extract quality concentration
Add.
Claims (5)
1. the present invention provides a kind of method that oxidation-resistant active ingredient is extracted from cauliflower, include the following steps：
Step 1：Cauliflower is taken, in 50 DEG C~60 DEG C of baking oven after dry 20h~24h, is crushed using micronizer,
Powder after crushing continues in 50 DEG C~60 DEG C of baking oven and dries 7h~10h；
Step 2：Dried cauliflower powder ultrasound extraction 6h~8h in 60%~75% ethanol is taken, ethanol volume is flower coconut palm
10~20 times of dish quality, filtering；Filter residue is continued in 60%~75% ethanol and filtered after ultrasound extraction 6h~8h, continuous 3 times,
Merge 3 filtrates；
Step 3：Filtrate is carried out rotation to be evaporated, vapors away ethanol, until filtrate does not have alcohol taste；
Step 4：Filtered after step 3 gained filtrate is diluted one times using 0.22 μm of filter membrane, collect filtrate, filtrate uses
1KDa ultrafiltration membranes, in 1 DEG C~5 DEG C, centrifugal ultrafiltration collects the component that molecular weight is less than 1KDa under conditions of 3000g~5000g, obtains
The component that antioxidation activity is most strong in cauliflower is obtained, after being freezed to the component, is configured to the solution of various concentrations gradient, is carried out
DPPH radicals scavengings are tested, and hydroxyl radical free radical removes experiment and its anti-oxidant work of ultra-oxygen anion free radical removing experimental verification
Property.
2. the separation according to claim 1 from cauliflower prepares the active ingredient with anti-oxidation efficacy, its feature exists
In step 1, the cauliflower drying temperature is 60 DEG C, drying time 20h；Ultramicro-powder is carried out to dried cauliflower
After broken, it is 60 DEG C to continue drying temperature, time 8h.
3. the separation according to claim 1 from cauliflower prepares the active ingredient with anti-oxidation efficacy, its feature exists
In step 2, concentration of alcohol used is 60%, and volume is 15 times of cauliflower quality.
4. the separation according to claim 1 from cauliflower prepares the active ingredient with anti-oxidation efficacy, its feature exists
In step 4, by the way of centrifugal ultrafiltration, using 1KDa ultrafiltration membranes, in 1 DEG C~5 DEG C, centrifugal ultrafiltration under conditions of 3000g
Collect the component with anti-oxidation efficacy that molecular weight is less than 1KDa.
5. the separation according to claim 1 from cauliflower prepares the active ingredient with anti-oxidation efficacy, its feature exists
In, in step 4, by from cauliflower separation prepare the component with anti-oxidation efficacy freeze, obtain in cauliflower have resist
The freeze-dried powder of the isolate of oxidation efficiency.
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| CN201711333462.5A CN107998167A (en) | 2017-12-14 | 2017-12-14 | A kind of method that oxidation-resistant active ingredient is extracted from cauliflower |
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| CN201711333462.5A CN107998167A (en) | 2017-12-14 | 2017-12-14 | A kind of method that oxidation-resistant active ingredient is extracted from cauliflower |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110559224A (en) * | 2019-08-30 | 2019-12-13 | 广东丸美生物技术股份有限公司 | Broccoli extract and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102295667A (en) * | 2011-06-28 | 2011-12-28 | 浙江省农业科学院 | Method for extracting glucoraphanin compound from broccoli and cauliflower |
| CN103709211A (en) * | 2013-12-13 | 2014-04-09 | 大兴安岭嘉迪欧营养原料有限公司 | Method for preparing glucoraphanin from broccoli |
-
2017
- 2017-12-14 CN CN201711333462.5A patent/CN107998167A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102295667A (en) * | 2011-06-28 | 2011-12-28 | 浙江省农业科学院 | Method for extracting glucoraphanin compound from broccoli and cauliflower |
| CN103709211A (en) * | 2013-12-13 | 2014-04-09 | 大兴安岭嘉迪欧营养原料有限公司 | Method for preparing glucoraphanin from broccoli |
Non-Patent Citations (1)
| Title |
|---|
| 尹鹏等: "花椰菜总黄酮提取工艺优化", 《南方农业学报》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110559224A (en) * | 2019-08-30 | 2019-12-13 | 广东丸美生物技术股份有限公司 | Broccoli extract and preparation method and application thereof |
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