CN107996411A - A kind of method of root induction in Chinese Gooseberry culture - Google Patents
A kind of method of root induction in Chinese Gooseberry culture Download PDFInfo
- Publication number
- CN107996411A CN107996411A CN201810083013.8A CN201810083013A CN107996411A CN 107996411 A CN107996411 A CN 107996411A CN 201810083013 A CN201810083013 A CN 201810083013A CN 107996411 A CN107996411 A CN 107996411A
- Authority
- CN
- China
- Prior art keywords
- culture
- root
- root induction
- plant
- chinese gooseberry
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The present invention relates to a kind of method of root induction in Chinese Gooseberry culture, propagation after reach more than 3cm, in the matrix of plant insertion the inside with more than 3 cotyledons, the matrix is MS+ vermiculites, light application time 16h, 1500 2000LX, 25 ± 2 DEG C of temperature, gas concentration lwevel 950ppm, humidity 80%, more than 1000 grades of culturing room's cleanliness factor.Culture forms the Kiwi berry intact plant of well developed root system after 20 days.The present invention is using MS and vermiculite as the environment of culture medium, preferably simulation Kiwi growth, stringent control light application time and intensity, while controls humidity and the concentration of carbon dioxide, and the plant Rapid Rooting after breeding for Kiwi berry provides excellent growing environment.Technical scheme breaches the problem of woody tissue-cultured seedling quality of rooting difference, and tissue-cultured seedling well developed root system, completely, survival rate are high, without hardening.
Description
Technical field
The present invention relates to field of plant tissue culture technique, root induction in more particularly to a kind of Chinese Gooseberry culture
Method.
Background technology
Kiwi berry (scientific name：Actinidia chinensis Planch), it is perennial bejuco, Kiwi berry
Soft texture, sweet mouthfeel.Taste is described as the mixing of strawberry, banana, pineapple three.Kiwi berry remove containing actinidine,
The organic matters such as proteolytic enzyme, tannin pectin and carbohydrate, and 17 kinds of the trace element such as calcium, potassium, selenium, zinc, germanium and needed by human body
Outside amino acid, also containing abundant vitamin C, grape acid, fructose, citric acid, malic acid, fat.
The propagation method of traditional Kiwi berry has sowing, cuttage, press strip and grafting etc..These method relative efficiencies are relatively low, mesh
Before, there are many methods using tissue cultures to carry out Kiwi berry breeding, such as：Patent CN102177847A discloses one kind
The factorial seedling-culturing method of tara vine, including the preparation of culture medium, the selection of explant and disinfection, inoculation, squamous subculture,
Sand bed transplants five steps.But this method needs to configure complicated component and the basic culture of culture medium configuration process requirement strictly
Base, inducing culture and subculture medium, operating process are cumbersome, it is necessary to more manpower and equipment cost be put into, without wide
General applicability.But in the method for existing tissue cultures.There are many shortcomings, are all to need to contain particularly in the stage of taking root
Sugar culture-medium, but sugar is pollution sources, the pollution rate in stage easy to increase of taking root.
Plant hormone would generally be added used by traditional root induction in culture medium, such as IBA, IAA, NAA, some are
Hestening rooting, can add 0.3% activated carbon, use is fluid nutrient medium mostly in the medium.It is common conviction that training
The generation that some inorganic salts ingredients in base are unfavorable for root is supported, suitably to reduce the differentiation that inorganic salt concentration is just conducive to root, because
This, a great number of elements will drop to 1/2 to 1/4 or so in using MS as the root media of minimal medium.Traditional method is taken root
When can only obtain 3-5 main root, and do not have root hair, as shown in Fig. 2, needing later stage hardening culture.
The content of the invention
For problems of the prior art, the object of the present invention is to provide life is induced in a kind of Chinese Gooseberry culture
The method of root.
In order to solve the above-mentioned technical problem, the present invention is achieved by the following scheme：
A kind of method of root induction in Chinese Gooseberry culture,
More than 3cm is reached after propagation, there is the matrix inside the special equipment of taking root of plant insertion of more than 3 cotyledons
On, which is MS+ vermiculites, light application time 16h, 1500-2000LX, 25 ± 2 DEG C of temperature, gas concentration lwevel 950ppm, wet
Degree 80%, more than 1000 grades of culturing room's cleanliness factor.Culture forms the Kiwi berry intact plant of well developed root system after 20 days.
MS+ vermiculites add 800g vermiculites for every liter of MS solution.
The present invention is used as the environment of culture medium, preferably simulation Kiwi growth, stringent control illumination using MS and vermiculite
Time and intensity, while humidity and the concentration of carbon dioxide are controlled, the plant Rapid Rooting after breeding for Kiwi berry provides excellent
Good growing environment.Technical scheme breaches the problem of woody tissue-cultured seedling quality of rooting difference, tissue-cultured seedling well developed root system,
Completely, survival rate is high, without hardening.The present invention is taken root device, it will thus provide the lamp of illumination is arranged inside device, makes light source from plant
Close, the illumination utilization rate higher of strain, and each device is all independent, easy to observe and control.
Brief description of the drawings：
Fig. 1 is：State after the completion of Kiwi berry root induction in embodiment 1；
Fig. 2 is：State after the completion of traditional tissue cultures root induction；
Fig. 3：The dimensional structure diagram of embodiment 3；
Fig. 4：The cross-sectional view of embodiment 3；
Fig. 5：The overlooking the structure diagram of root bed described in embodiment 3.
Embodiment
Below in conjunction with preferred embodiment, to according to embodiment provided by the invention, details are as follows：
Embodiment 1
Using Xu Xiang kiwi fruit leafs as group training material, tissue cultures are carried out using the method for the present invention.
1. emerald green, thick and solid in the healthy and strong kiwi fruit plant of selection, the blade closer from stem apex does explant material, and clear water is washed
Totally；
With 0.1% mercuric chloride solution sterilize 10min, then with 75% alcohol sterilize 5min, sterile water wash 3-5 times；
Blade after processing is cut into the square of length of side 1.5cm sizes, is put on inducing culture,
Illumination 800LX, daily 10h, 25 ± 2 DEG C of temperature, humidity 70%, cultivates 50 days or so and forms Kiwi berry callus group
Knit.
Inducing culture is：MS culture mediums+sucrose 20g/L+ agar 6g/L+6-BA0.8mg/L+NAA (methyl α-naphthyl acetate)
The culture medium of 0.2mg/L+2,4-D (2,4 dichlorophenoxyacetic acid) 0.5mg/L；
2. induce Multiple Buds
Callus is divided into the fritter of diameter 1cm or so, disposes dead leaf tissue, be partly embedded into MS+
In the culture medium of sucrose 30g/L+ agar 6g/L+6-BA5mg/L+NAA0.5mg/L+IAA0.2mg/L, illumination 2000-2500LX,
Time 14h, 25 ± 2 DEG C of temperature, humidity 70%, callus surface forms adventitious bud after cultivating 50 days, obtains primary.
3. squamous subculture (Multiplying culture) is the adventitious bud insertion MS+ sucrose 30g/L+ agar 6g/L formed on callus
On the culture medium of+6-BA1.2mg/L+IAA0.8mg/L, illumination 2000-3000LX, 14h, 25 ± 2 DEG C of temperature, humidity 70%, is trained
Support 25 days, 3-5 times of appreciation rate.
4. more than 3cm is reached after propagation, the plant with more than 3 cotyledons is inserted into special equipment of taking root for root induction
In matrix inside (embodiment 3), which is MS+ vermiculites, light application time 16h, 1500-2000LX, 25 ± 2 DEG C of temperature, two
Aoxidize concentration of carbon 950ppm, humidity 80%, more than 1000 grades of culturing room's cleanliness factor.Culture forms the Mi of well developed root system after 20 days
Monkey peach intact plant.As shown in Figure 1.
5. a Kiwi berry intact plant is transplanted to inside degraded bag, warm canopy is transplanted to, 15-30 DEG C of temperature, humidity 85%, one
Crop field, survival rate more than 99% can be moved on to after week.
Embodiment 2
Using Xu Xiang kiwi fruit leafs as group training material, tissue cultures are carried out using different methods.
Experimental group：Use the method for 1 step 4 root induction of the embodiment of the present invention；
Compare 1 group：Culture medium is used by root induction step：0.7mg·L-1IBA、30mg·L-1Sucrose, 7mg
L-1The 1/2MS culture mediums of agar, other are identical with experimental group.
Compare 2 groups：Culture medium is used by root induction step：In 1/2MS+NAA0.2mgL-1, other and experiment
Group is identical.
Compare 3 groups：Light application time 10h used by root induction step, 25 ± 2 DEG C of temperature, humidity 85%, other and reality
It is identical to test group.
Compare 4 groups：Gas concentration lwevel 1200ppm used by root induction step, other are identical with experimental group.
| Take root pollution rate | Transplanting survival rate | Take root situation | Whether hardening | |
| Experimental group | 0.5% | 99% | It is good | It is no |
| Compare 1 group | 5% | 86% | Generally | It is |
| Compare 2 groups | 6% | 91% | Generally | It is |
| Compare 3 groups | 1% | 95% | Preferably | It is no |
| Compare 4 groups | 1% | 93% | Generally | It is |
Embodiment 3
A kind of special equipment of taking root of tissue cultures, as shown in Figures 3 to 5, including：Box body 1, box lid 2 and root bed 3, it is described
Box lid 2 is connected by hinge 26 with box body 1, can be overturn with respect to box body 1；Described bed 3 is by some orthogonal partition plate groups
Into described bed 3 has the root bed region 30 for being used to house culture medium of several mutually independent groined types, the box lid 2
Bottom surface on be provided with LED light 20, some ventilation holes 10 are offered in the side wall of the box body 1, residing for the ventilation hole 10
Position is higher than the top surface of root bed 3.The root that macaque peach seedling is grown can all be separated by root bed 3, after the completion of avoiding training seedling, it
Warped roots in together, time-consuming and laborious when they are separated and easy damaged root system.
The above described is only a preferred embodiment of the present invention, being not the limitation for making other forms to the present invention, appoint
What those skilled in the art changed or be modified as possibly also with the technology contents of the disclosure above equivalent variations etc.
Imitate embodiment.But it is every without departing from technical solution of the present invention content, the technical spirit according to the present invention is to above example institute
Any simple modification, equivalent variations and the remodeling made, still fall within the protection domain of technical solution of the present invention.
Claims (4)
1. a kind of method of root induction in Chinese Gooseberry culture, it is characterised in that
Reach more than 3cm after breeding in tissue cultures, there is the plant of more than 3 cotyledons, be inserted into matrix, which is MS
+ vermiculite；Light application time 16h is controlled, 1500-2000LX, 25 ± 2 DEG C, gas concentration lwevel 950ppm of temperature, humidity 80%, is trained
Support room cleanliness factor more than 1000 grades；Culture forms the Kiwi berry intact plant of well developed root system after 20 days.
2. the method for root induction in Chinese Gooseberry culture according to claim 1, it is characterised in that MS+ vermiculites are
Every liter of MS solution adds 800g vermiculites.
3. the method for root induction in Chinese Gooseberry culture according to claim 1 or 2, it is characterised in that this method
Available for root induction in the tissue cultures of any xylophyta.
4. the method for root induction in Chinese Gooseberry culture according to claim 3, it is characterised in that this method can use
In Kiwi berry, apple, cherry tissue cultures in root induction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810083013.8A CN107996411A (en) | 2018-03-02 | 2018-03-02 | A kind of method of root induction in Chinese Gooseberry culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810083013.8A CN107996411A (en) | 2018-03-02 | 2018-03-02 | A kind of method of root induction in Chinese Gooseberry culture |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107996411A true CN107996411A (en) | 2018-05-08 |
Family
ID=62066549
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810083013.8A Pending CN107996411A (en) | 2018-03-02 | 2018-03-02 | A kind of method of root induction in Chinese Gooseberry culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107996411A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114766367A (en) * | 2022-05-18 | 2022-07-22 | 西北农林科技大学 | Kiwi fruit tissue culture method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845313A (en) * | 2012-10-19 | 2013-01-02 | 沈阳农业大学 | Method for quickly in-vitro actinidia kolomikta propagating |
| CN107996410A (en) * | 2018-01-29 | 2018-05-08 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using Kiwi berry blade |
-
2018
- 2018-03-02 CN CN201810083013.8A patent/CN107996411A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102845313A (en) * | 2012-10-19 | 2013-01-02 | 沈阳农业大学 | Method for quickly in-vitro actinidia kolomikta propagating |
| CN107996410A (en) * | 2018-01-29 | 2018-05-08 | 宝鸡松良农业科技有限公司 | A kind of method that tissue cultures are carried out using Kiwi berry blade |
Non-Patent Citations (3)
| Title |
|---|
| 朱道圩等: "猕猴桃实生苗组织培养体系建立的研究 ", 《落叶果树》 * |
| 王碧琴: "不同固化物对中华猕猴桃胚乳试管苗生根的影响 ", 《江西林业科技》 * |
| 陈玉霞: "猕猴桃试管苗培养基的改进效果研究 ", 《湖北农业科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114766367A (en) * | 2022-05-18 | 2022-07-22 | 西北农林科技大学 | Kiwi fruit tissue culture method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101258835B (en) | Fast reproducing method for high quality seedling of dendrobium officinale | |
| CN103931492B (en) | The tissue culture fast seedling-cultivating method of apple rootstock M9 | |
| CN101647393B (en) | Fast tissue culture reproducing method of actinidia eriantha | |
| CN101491215B (en) | Chinese toon tissue-culture quick propagation technique | |
| CN101926287B (en) | Method for culturing tissue of 'Zhongzhen No.1' | |
| CN102150624A (en) | Tissue culture and rapid propagation method of pinellia genus plant | |
| CN102217539A (en) | Isolated rooting culture method for fir clone | |
| CN108271691A (en) | A method of carrying out tissue cultures using Kiwi berry stem | |
| CN111616052A (en) | Rapid propagation and sugar-free rooting culture method and application of apple rootstock catalpa bungei | |
| CN106212274A (en) | The method for tissue culture of special beautiful Caulis et folium euphorbiae milii | |
| CN1799344A (en) | Artificial quick propagation method for hemsley rockvine root | |
| CN108770695B (en) | Method for in-vitro tissue culture and rapid seedling establishment of red-heart pitaya | |
| CN107996410A (en) | A kind of method that tissue cultures are carried out using Kiwi berry blade | |
| CN102907326B (en) | Tissue culture propagation method for Medicagao Sativa L. | |
| CN103988776A (en) | Nantong xiaofang persimmon tissue culture rapid propagation method | |
| CN103947548A (en) | Method for establishing agapanthus high-frequency regeneration system | |
| CN106106178A (en) | A kind of method for tissue culture of confection Rhizoma Iridis Tectori | |
| CN101015280B (en) | Tissue culture method for fast propagation of primula denticulata ssp.sino-denticulata | |
| CN103155869A (en) | Sweet cherry rootstock Colt tissue culture method | |
| CN108124773A (en) | A kind of method that tissue cultures are carried out using apple stem section | |
| CN107996411A (en) | A kind of method of root induction in Chinese Gooseberry culture | |
| CN101015279B (en) | Tissue culture method for fast propagation of primula poissonii | |
| CN101658133A (en) | Method for quickly and directly establishing albizzia regeneration plant system | |
| CN106857265B (en) | A kind of tissue culture enrichment procedure of white Cowhells wood | |
| CN104285816A (en) | Rapid propagation method for xanthoceras sorbifolia bunge tissue during culturing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20200731 Address after: 722300 Baoji County, Shaanxi Province Meixian (state) kiwifruit Industrial Park testing center two floor Applicant after: BAOJI SONGLIANG AGRICULTURAL TECHNOLOGY Co.,Ltd. Applicant after: Shaanxi Pufeng Modern Agriculture Co., Ltd Address before: 722300 Baoji County, Shaanxi Province Meixian (state) kiwifruit Industrial Park testing center two floor Applicant before: BAOJI SONGLIANG AGRICULTURAL TECHNOLOGY Co.,Ltd. |
|
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180508 |