CN107991480A - A kind of kit of chemiluminescence detection food security and preparation method thereof - Google Patents
A kind of kit of chemiluminescence detection food security and preparation method thereof Download PDFInfo
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- CN107991480A CN107991480A CN201810091919.4A CN201810091919A CN107991480A CN 107991480 A CN107991480 A CN 107991480A CN 201810091919 A CN201810091919 A CN 201810091919A CN 107991480 A CN107991480 A CN 107991480A
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- famoxate
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
- G01N33/54326—Magnetic particles
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Abstract
The invention discloses a kind of with kit of chemiluminescence detection food security and preparation method thereof.The kit includes：Famoxate antigen or antibody, the antibody or antigen, Famoxate serial standards solution, chemiluminescence preexciting liquid A, chemiluminescence exciting liquid B, cleaning solution of magnetic particle coupling of acridinium ester label.Chemiluminescence is combined by kit of the present invention with immune magnetic particle, there is provided a kind of close to homogeneous reaction system.Compared with prior art, kit of the present invention has the advantages that high sensitivity, high specificity, reaction time are short.
Description
Technical field
The invention belongs to field of detection of food safety, is specifically a kind of Famoxate in chemiluminescence detection food security
Remaining kit and preparation method thereof.
Background technology
Famoxate, outer literary fame Famoxadone, chemical formula is C22H18N2O4.Famoxate is new and effective, wide spectrum
Sterilization pesticide.Suitable for crop such as wheat, barley, pea, beet, rape, grape, potato, pawl class, capsicum, tomato etc..Mainly
For preventing the important disease in Ascomycetes, Basidiomycetes, oomycetes subclass such as powdery mildew, rust, glume blight, net blotch, frost
Mildew, late blight etc..It is mixed with Flusilazole more preferable to prevention wheat Ying blight, net blotch, powdery mildew, rust effect, have and protect
Protect, treat, rooting out, permeating, systemic activity.Mechanism of action is inhibitor of energy, i.e. Mitochondrial electron transmission suppresses system.With parent
Lipid, after spraying on crop leaf, easily adhesion, not by rain drop erosion special efficacy.But Famoxate pesticide residue is most in food
There are clear and definite relevant regulations in big residue limits various countries, for ensuring food safety.Famoxate residual quantity in soil
Measure also has beneficial effect.
The method of Famoxate detection at present has：Microbial method, enzyme-linked immunization, high performance liquid chromatography, colloidal gold method,
Chemoluminescence method.Microbial method poor specificity, cumbersome, detection cycle is longer, and resultant error is larger.Using enzyme linked immunological
The shortcomings that method is detected is that peroxidase or alkaline phosphatase label easy in inactivation, chromogenic substrate are shown in that light easily decomposes, and sensitivity is low,
The compound similar to structure has certain cross reaction, causes test result inaccurate, and be only applicable to micro substance
Detection and identification.The shortcomings that using high performance liquid chromatography is complicated for sample pre-treatments, and laborious time-consuming, testing cost is high.Adopt
The shortcomings that being detected with colloidal gold method is that last testing result is characterized with macroscopic color, and error is larger, and
And it is cumbersome, flow is more, is more easy to error occur, sensitivity is low.The present invention uses acridinium ester label, has the following advantages that：1
Background luminescence is low, and signal-to-noise ratio is high, and luminescence-producing reaction disturbing factor is few；2 flash types shine, light release is quickly concentrated, luminous efficiency is high,
Luminous intensity is big；3 acridinium ester molecular weight are small, avoid masking antibody combining site, improve system overall sensitivity；4 are easy to and egg
Photon yield is not reduced after white matter is coupled and is coupled；5 labels are stablized, can at 2-8 DEG C from the influence of ambient oxidant
Preserve the several months long.Therefore acridine substituent is a kind of very effective chemiluminescent labels.
The content of the invention
There is Famoxate pesticide residue in higher sensitivity and specific detection food the present invention provides a kind of
Kit.
To achieve the above object, the present invention provides following technical solution：
A kind of kit of chemiluminescence detection food security and preparation method thereof, chemiluminescence provided by the present invention
Method detects the kit of Famoxate pesticide residue, can use Famoxate antibody coupling magnetic particle, acridinium ester label oxazole
Bacterium ketone, can also use Famoxate antigen coupling magnetic particle, acridinium ester label Famoxate antibody.Kit further includes oxazole
Bacterium ketone calibration object, chemiluminescence preexciting liquid A, chemiluminescence exciting liquid B and cleaning solution；Wherein described Famoxate antigen is
Artificial synthetic polypeptide antigen, its amino acid sequence are Glu Lys Lys Gly Ile Gln Gly Ser Thr Ala Lys
Ser。。
Preferably, the chemiluminescent labels are acridinium ester, such as NSP-DMAE, NSP-SA-NHS.
Preferably, the acridinium ester label is Famoxate antigen or Famoxate antibody.
Preferably, the mark buffer solution of the acridinium ester label Famoxate antigen or antibody is ph 9.0-11.0, dense
Spend sodium carbonate-ammonium hydrogen carbonate for 0.1mol/L, preferably ph 10.0.
Preferably, the molar ratio of the acridinium ester and antigen or antibody is 5-100:1, preferred molar ratio 7.4:
1。
Preferably, the acridinium ester is dissolved in DMSO or DMF, and the ultimate density of prepared solution is 0.1-
100mmol/L, preferred concentration 5.5mmol/L.
Preferably, the magnetic particle can be directly coupled with Famoxate antibody or antigen, also can be by magnetic particle and chain
Mould Avidin coupling, while use biotin labeling Famoxate antibody or antigen.
Preferably, the surface modification group of the magnetic bead is carboxyl, amino, Streptavidin-biotin or to toluene
Sulfonyl.Magnetic bead uses the heroic accuracy of endless bulk deposition meeting of preceding solid phase, therefore should select good dispersion during selection, puts for a long time
It is few to put magnetic bead number of uniting, the slow magnetic bead of sinking speed.
Preferably, the calibration object is with containing 0.5-5.0% bovine serum albumin(BSA)s and 0.1-0.5%PC300
Tromethamine buffer solution is matrix, adds Famoxate sterling and is formulated, calibration object form is liquid.
Preferably, the Famoxate calibration object solution concentration is respectively：0μg/L、0.4μg/L、2 μg/L、10μ
g/L、50μg/L、250μg/L。
Preferably, chemiluminescence preexciting liquid A of the present invention is the mixed liquor of hydrogen peroxide and nitric acid, wherein dioxygen
Water quality fraction is 0.05-5%, concentration of nitric acid 0.05-2.5mol/L.
Preferably, chemiluminescence preexciting liquid B of the present invention is polyethylene glycol octyl group methyl phenyl ethers anisole and sodium hydroxide
Mixed liquor, the wherein concentration of polyethylene glycol octyl group methyl phenyl ethers anisole is 0.05-2.0mol/L, and the concentration of sodium hydroxide is 0.05-
1.0mol/L。
Preferably, the cleaning solution includes following components：Ph values 7.0-9.0, concentration are 5.0-50.0mmol/L's
Tromethamine solution, wherein the sodium chloride and mass fraction that are 0.05-0.50mmol/L containing concentration are spitting for 0.01-0.25%
Temperature 20.
The principle of the present invention is to be combined the high degree of specificity of antibody-antigene reaction with the high sensitivity that acridinium ester shines
Get up, the photon detection production concentration produced using acridinium ester catching reaction.
Beneficial effects of the present invention：Competition law joint magnetic microparticle chemiluminescence technology is used to measure the oxazole bacterium in food
The content of ketone pesticide residue, and Famoxate antigen is artificial synthetic polypeptide antigen.Direct chemistry of the acridinium ester as label
Shine and have a clear superiority, be mainly manifested in：Catalyst is not required in reaction, as long as alkaline environment can carry out, is swift in response, can
The photon produced with complete catching reaction, background luminescence is low, and signal-to-noise ratio is high, and disturbing factor is few, and reagent stability is good, system letter
Single, exciting liquid cost is low.
Embodiment
The present invention provides a kit with chemiluminescence detection food security, to make the object of the invention, technical side
Case and effect are clearer, clear and definite, and the present invention is described in detail below.
The present invention provides a kind of kit of chemiluminescence detection food security and preparation method thereof, wherein, the present invention
The kit of the chemiluminescence method detection Famoxate pesticide residue provided, can take Famoxate antibody coupling magnetic micro-
Grain, acridinium ester label Famoxate can also use Famoxate antigen coupling magnetic particle, and acridinium ester label Famoxate resists
Body.Kit further includes Famoxate calibration object, chemiluminescence preexciting liquid A, chemiluminescence exciting liquid B and cleaning solution.Dislike
Cycloheximide triazole antigen is artificial synthetic polypeptide antigen, its amino acid sequence is Glu Lys Lys Gly Ile Gln Gly Ser
Thr Ala Lys Ser。
Specifically, the present invention is during magnetic particle coupling is prepared, the buffer solution of the coupled antigen is ph values 6.0,
Concentration is the buffer solution of 0.1mol/L MES；The buffer solution of the coupled antibody is ph values 6.5, concentration is 0.1mol/L MES
Buffer solution.
Specifically, for the present invention during magnetic particle coupled antigen or antibody is prepared, the Block buffer is containing 1%
The buffer solution of bovine serum albumin(BSA).
Specifically, calibration object of the present invention is with the ammonia fourth three containing 1% bovine serum albumin(BSA) and 0.1%PC300
Alcohol buffer solution is matrix, adds Famoxate sterling and is formulated, calibration object form is liquid.
Specifically, chemiluminescence preexciting liquid A of the present invention is the mixed liquor of hydrogen peroxide and nitric acid, wherein hydrogen peroxide
Mass fraction is 1.5%, concentration of nitric acid 0.1mol/L.
Specifically, chemiluminescence exciting liquid B of the present invention is the mixed of polyethylene glycol octyl group methyl phenyl ethers anisole and sodium hydroxide
Liquid is closed, the wherein concentration of polyethylene glycol octyl group methyl phenyl ethers anisole is 0.1mol/L, and the concentration of sodium hydroxide is 0.35mol/L.
Specifically, the tromethamine solution that cleaning solution of the present invention is ph values 7.2, concentration is 25mmol/L, wherein containing
There are the sodium chloride that concentration is 0.15mmol/L and the polysorbas20 that mass fraction is 0.05%.
Below by embodiment, the present invention is described in detail.
Embodiment 1：A kind of establishment and preparation method 1. kit 1 of kit 1 with chemiluminescence detection food security
Establishment
The Famoxate antigen of acridinium ester label；
The Famoxate antibody of magnetic particle coupling；
Famoxate series of calibration product solution, concentration are respectively：0μg/L、0.4μg/L、2μg/L、10 μg/L、50μg/L、
250μg/L；
Chemiluminescence preexciting liquid A and chemiluminescence exciting liquid B；
Cleaning solution, including following components：Ph values 7.2, the tromethamine solution that concentration is 25mmol/L, wherein containing concentration
The polysorbas20 that sodium chloride and mass fraction for 0.15mmol/L are 0.05%.
2. it is coupled the preparation of the magnetic particle suspension of Famoxate antibody
(1) 1mg carboxyls magnetic particle is taken in 0.5mL centrifuge tubes, adds the MES buffer solutions of a certain amount of 0.1mol/L, is vortexed
Mix, be placed on magnetic frame, standing 5min makes magnetic particle be separated with liquid, and supernatant discarding, washs 3 times, add a certain amount of MES
(ph 6.0) buffer solution, is vortexed.
(2) 15 μ L (15 μ g) Famoxate antibody is added, is vortexed, revolving reaction pipe, is incubated at room temperature 17min.
(3) add the coupling reagent EDC that 10 μ L concentration are 10mg/mL to be vortexed, revolving reaction pipe, is incubated at room temperature 2h.
(4) supernatant is removed, adds the cleaning buffer solution (TBS+0.05% polysorbas20s) of 200 μ L, is washed 3 times.
(5) closed, closed 4 times repeatedly, each 10min with containing 1% bovine serum albumin(BSA) buffer solution.By the magnetic particle
Suspension is placed in 2-8 DEG C of preservation.
3. the preparation of acridinium ester label Famoxate antigen
(1) Famoxate antigen is purified：A certain amount of Famoxate antigen is placed in bag filter to (Famoxate antigen is
Artificial synthetic polypeptide antigen, its amino acid sequence are Glu Lys Lys Gly Ile Gln Gly Ser Thr Ala Lys
Ser), and bag filter is placed in the mark buffer solution not less than 1L and dialysed, during which buffer solution at least replaces 3 times, successively finally
Dialysed overnight, the sodium carbonate-bicarbonate buffer solution that mark buffer solution is ph10.1, concentration is 0.1mol/L.
(2) the acridinium ester NSP-DMAE-NHS of 1.6mg is weighed, is dissolved in anhydrous dimethyl formamide DMF, is made into
The NSP-DMAE-NHS DMF solutions of 6.5mmol/L.
(3) the Famoxate antibody after dialysis is placed in 1.5mL centrifuge tubes (lucifuge reaction), adds 200 μ L
Ph10.0, the sodium carbonate-bicarbonate buffer solution that concentration is 0.1mol/L, add the NSP-DMAE- of 195 μ L, 6.5mmol/L
NHS DMF solutions, react 0.5h at room temperature, add 100 μ L of 10g/L lysines, the reaction was continued 15min, make mark reaction terminating.
(4) label NSP-DMAE-NHS-Ag and free NSP-DMAE-NHS by Sephadex G-50 columns (1 ×
25cm) separate, the PBS for being 0.1mol/L with purification buffer ph 6.3, concentration is balanced and eluted chromatographic column.
(5) protein peak is detected with chromatograph in separation process, measures the chemiluminescence intensity and 280nm of efflux respectively
Absorbance.
(6) eluent that shading value is high and absorbance is big is collected, adds 1% bovine serum albumin(BSA) (volume) packing frost afterwards
Preserve.
4. the preparation of Famoxate series of calibration product solution
With being matrix containing the tromethamine buffer solution containing 1% bovine serum albumin(BSA) and 0.1%PC300, oxazole is added
Bacterium ketone sterling is made into sign concentration：0μg/L、0.4μg/L、2μg/L、10μg/L、 50μg/L、250μg/L.
5. the preparation of shine exciting liquid A, B
(1) chemiluminescence preexciting liquid A is made of the mixed liquor of hydrogen peroxide and nitric acid.Wherein hydrogen peroxide mass fraction is
1.5%, concentration of nitric acid 0.1mol/L, are distributed into 20mL/ branch, 2-8 DEG C saves backup with brown bottle.
(2) chemiluminescence exciting liquid B is made of the mixed liquor of polyethylene glycol octyl group methyl phenyl ethers anisole and sodium hydroxide.Wherein poly- second
The concentration of glycol octyl group methyl phenyl ethers anisole is 0.1mol/L, and the concentration of sodium hydroxide is 0.35mol/L, and 20mL/ is distributed into brown bottle
Branch, 2-8 DEG C saves backup.
Embodiment 2：A kind of establishment of kit 1 with chemiluminescence detection food security and preparation method
1. the establishment of kit 2
The Famoxate antigen of acridinium ester label；
The Famoxate antibody of magnetic particle coupling；
Famoxate series of calibration product solution, concentration are respectively：0μg/L、0.4μg/L、2μg/L、10 μg/L、50μg/L、
250μg/L；
Chemiluminescence preexciting liquid A and chemiluminescence exciting liquid B；
Cleaning solution, including following components：Ph values 7.2, the tromethamine solution that concentration is 25mmol/L, wherein containing concentration
The polysorbas20 that sodium chloride and mass fraction for 0.15mmol/L are 0.05%.
2. it is coupled the preparation of the magnetic particle suspension of Famoxate antibody
(1) 1mg carboxyls magnetic particle is taken in 0.5mL centrifuge tubes, adds the MES buffer solutions of a certain amount of 0.1mol/L, is vortexed
Mix, be placed on magnetic frame, standing 5min makes magnetic particle be separated with liquid, and supernatant discarding, washs 3 times, add a certain amount of MES
(ph 6.0) buffer solution, is vortexed.
(2) 25 μ L (25 μ g) Famoxate antibody is added, is vortexed, revolving reaction pipe, is incubated at room temperature 17min.
(3) add the coupling reagent EDC that 10 μ L concentration are 10mg/mL to be vortexed, revolving reaction pipe, is incubated at room temperature 2h.
(4) supernatant is removed, adds the cleaning buffer solution (TBS+0.05% polysorbas20s) of 200 μ L, is washed 3 times.
(5) closed, closed 4 times repeatedly, each 10min with containing 1% bovine serum albumin(BSA) buffer solution.By the magnetic particle
Suspension is placed in 2-8 DEG C of preservation.
3. the preparation of acridinium ester label Famoxate antigen
(1) Famoxate antigen is purified：A certain amount of Famoxate antigen is placed in bag filter, and bag filter is placed in
Dialyse in mark buffer solution not less than 1L, during which buffer solution at least replaces 3 times, finally dialysed overnight successively, marks buffer solution
It is ph10.1, the sodium carbonate-bicarbonate buffer solution that concentration is 0.1mol/L.
(2) the acridinium ester NSP-DMAE-NHS of 1.6mg is weighed, is dissolved in anhydrous dimethyl formamide DMF, is made into
The NSP-DMAE-NHS DMF solutions of 6.5mmol/L.
(3) the Famoxate antibody after dialysis is placed in 1.5mL centrifuge tubes (lucifuge reaction), adds 200 μ L
Ph10.0, the sodium carbonate-bicarbonate buffer solution that concentration is 0.1mol/L, add the NSP-DMAE- of 195 μ L, 6.5mmol/L
NHS DMF solutions, react 0.5h at room temperature, add 100 μ L of 10g/L lysines, the reaction was continued 15min, make mark reaction terminating.
(4) label NSP-DMAE-NHS-Ag and free NSP-DMAE-NHS by Sephadex G-50 columns (1 ×
25cm) separate, the PBS for being 0.1mol/L with purification buffer ph 6.3, concentration is balanced and eluted chromatographic column.
(5) protein peak is detected with chromatograph in separation process, measures the chemiluminescence intensity and 280nm of efflux respectively
Absorbance.
(6) eluent that shading value is high and absorbance is big is collected, adds 1% bovine serum albumin(BSA) (volume) packing frost afterwards
Preserve.
4. the preparation of Famoxate series of calibration product solution
It is matrix with the tromethamine buffer solution containing 1% bovine serum albumin(BSA) and 0.1%PC300, adds Famoxate
Sterling is made into sign concentration：0μg/L、0.4μg/L、2μg/L、10μg/L、50 μg/L、250μg/L.
5. the preparation of shine exciting liquid A, B
(1) chemiluminescence preexciting liquid A is made of the mixed liquor of hydrogen peroxide and nitric acid.Wherein hydrogen peroxide mass fraction is
1.5%, concentration of nitric acid 0.1mol/L, are distributed into 20mL/ branch, 2-8 DEG C saves backup with brown bottle.
(2) chemiluminescence exciting liquid B is made of the mixed liquor of polyethylene glycol octyl group methyl phenyl ethers anisole and sodium hydroxide.Wherein poly- second
The concentration of glycol octyl group methyl phenyl ethers anisole is 0.1mol/L, and the concentration of sodium hydroxide is 0.35mol/L, and 20mL/ is distributed into brown bottle
Branch, 2-8 DEG C saves backup.
Embodiment 3：The detection of Famoxate residual quantity in sample
A. the processing of honey sample
Take honey 1.0g to add in 25mL centrifuge tubes, add 2mL and be vibrated to honey all dissolvings with vibrator, in 55-75
DEG C hot water in warm several minutes, add the sodium hydroxide that 5mL concentration is 0.2mol/L, it is 4-6 to adjust ph values scope, is added
The ethyl acetate of 8mL, vibration mix.4mL upper organic phases are taken to be blown into glass tube clean 10mL under 50 DEG C of nitrogen streams
Dry, it is 0.02 phosphate buffer that 0.5mL concentration, which is added dropwise, is mixed, to be checked.
B. the processing of milk sample
150 μ L fresh milks are taken in 500 μ L centrifuge tubes, 4 DEG C of centrifugation 10min (3000rpm), discard upper-layer fat.Move
Take the 25 μ L of milk sample after centrifugation to be placed in clean teat glass, add 950 μ L concentration and delay for 0.02mol/L phosphate
Fliud flushing is diluted.
Embodiment 4：With kit detection and interpretation of result
(1) by 1000 μ L of sample to be tested, 150 μ L of coupling magnetic particle suspension, 150 μ L of acridinium ester label, sequentially add
In reaction tube, vibration mixes, 37 DEG C of incubation 15min.
(2) separation cleaning 5 times.
(3) fully vibrating the reaction vessel after washing makes magnetic particle scatter.
(4) 100 μ L chemiluminescence exciting liquid B are added after adding 100 μ L chemiluminescence preexciting liquid A, 1s, measure its phase
To luminous intensity, the content of Famoxate luminous intensity proportion relation corresponding thereto in sample.
Embodiment 5：The test limit of kit
20 retests are carried out to zero standard solution, the standard deviation for taking the average value that zero standard solution measures to add 3 times
Difference, is the sensitivity of this kit.Sensitivity of this kit to Famoxate is 0.05 μ g/L.
Above example is only several embodiments of the present invention, but the present invention is not limited to this.Based in the present invention
Embodiment, those of ordinary skill in the art's obtained every other reality on the premise of any creative work is not made
Mode is applied, belongs to the scope that the present invention is protected.
Sequence table
<110>Li Xiumei
<120>A kind of kit of chemiluminescence detection food security and preparation method thereof
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 12
<212> PRT
<213>Artificial sequence (Artificial Sequence)
<400> 1
Glu Lys Lys Gly Ile Gln Gly Ser Thr Ala Lys Ser
1 5 10
Claims (6)
- A kind of 1. kit with chemiluminescence detection food security, it is characterised in that：Composition includes the evil of acridinium ester label Cycloheximide triazole antigen, the antibody of magnetic particle coupling, Famoxate serial standards solution, chemiluminescence preexciting liquid A, chemiluminescence Exciting liquid B, cleaning solution；Or composition includes the Famoxate antibody of acridinium ester label, is coupled the magnetic for having Famoxate antigen Particle, Famoxate serial standards solution, chemiluminescence preexciting liquid A, chemiluminescence exciting liquid B, cleaning solution；The oxazole Bacterium ketone antigen is artificial synthetic polypeptide antigen, its amino acid sequence is Glu Lys Lys Gly Ile Gln Gly Ser Thr Ala Lys Ser。
- 2. the kit according to claim 1 with chemiluminescence detection food security, it is characterised in that：The chemistry hair Signal thing is acridinium ester, such as NSP-DMAE, NSP-SA-NHS.
- A kind of 3. kit with chemiluminescence detection food security according to claim 1, it is characterised in that：Described Acridinium ester label is Famoxate antigen.
- A kind of 4. kit with chemiluminescence detection food security according to claim 1, it is characterised in that：Described Magnetic particle and Streptavidin or can be coupled, while use biotin labeling by magnetic particle directly with Famoxate antibody coupling Famoxate antibody.
- A kind of 5. kit with chemiluminescence detection food security according to claim 1, it is characterised in that：Described Calibration object is using the tromethamine buffer solution containing 0.5-5.0% bovine serum albumin(BSA)s and 0.1-0.5%PC300 as matrix, is added Enter the calibration object solution for the series concentration gradient that Famoxate sterling is formulated.
- A kind of 6. kit with chemiluminescence detection food security according to claim 1, it is characterised in that：Described Chemiluminescence preexciting liquid A is made of the mixed liquor of hydrogen peroxide and nitric acid, and chemiluminescence exciting liquid B is by polyethylene glycol octyl group benzene first The mixed liquor of ether and sodium hydroxide forms.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810091919.4A CN107991480A (en) | 2018-01-30 | 2018-01-30 | A kind of kit of chemiluminescence detection food security and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810091919.4A CN107991480A (en) | 2018-01-30 | 2018-01-30 | A kind of kit of chemiluminescence detection food security and preparation method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN107991480A true CN107991480A (en) | 2018-05-04 |
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ID=62039960
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810091919.4A Withdrawn CN107991480A (en) | 2018-01-30 | 2018-01-30 | A kind of kit of chemiluminescence detection food security and preparation method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116376847A (en) * | 2023-05-15 | 2023-07-04 | 江南大学 | Hybridoma cell strain secreting oxazolidone monoclonal antibody and application thereof |
| CN118091145A (en) * | 2024-04-28 | 2024-05-28 | 江西赛基生物技术有限公司 | Acridinium ester conjugate concentrated solution, working solution and application thereof |
-
2018
- 2018-01-30 CN CN201810091919.4A patent/CN107991480A/en not_active Withdrawn
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116376847A (en) * | 2023-05-15 | 2023-07-04 | 江南大学 | Hybridoma cell strain secreting oxazolidone monoclonal antibody and application thereof |
| CN116376847B (en) * | 2023-05-15 | 2024-05-24 | 江南大学 | Hybridoma cell strain secreting famoxadone monoclonal antibody and application thereof |
| CN118091145A (en) * | 2024-04-28 | 2024-05-28 | 江西赛基生物技术有限公司 | Acridinium ester conjugate concentrated solution, working solution and application thereof |
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Application publication date: 20180504 |