CN107988282A - A kind of method that glutamic acid is extracted from corn protein powder - Google Patents
A kind of method that glutamic acid is extracted from corn protein powder Download PDFInfo
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- CN107988282A CN107988282A CN201711323865.1A CN201711323865A CN107988282A CN 107988282 A CN107988282 A CN 107988282A CN 201711323865 A CN201711323865 A CN 201711323865A CN 107988282 A CN107988282 A CN 107988282A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/14—Glutamic acid; Glutamine
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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Abstract
The present invention provides a kind of method that glutamic acid is extracted from corn protein powder, is related to chemical technology field, comprises the following steps：The pretreatment of corn protein powder；The fermentation process of corn protein powder；The extraction purification of glutamic acid.The present invention provides a kind of method that glutamic acid is extracted from corn protein powder, is cleaned, deodorized, degerming to ultra-fine corn protein powder using supercritical carbon dioxide extraction method, improves the quality of corn protein powder；By the use of isopropanol as precipitating reagent, under low-temperature vacuum environment, promote the precipitation of glutamic acid, improve the preparation efficiency of glutamic acid, and isopropanol is reusable, reduces cost；By the use of corn protein powder as raw materials for production, price is cheaper, substantially reduces production cost, while product purity made from the method for the present invention is very high, and the process conditions of production are relatively simple, are more suitable for use of large-scale production.
Description
Technical field
The present invention relates to chemical technology field, more particularly to a kind of method that glutamic acid is extracted from corn protein powder.
Background technology
Glutamic acid, is a kind of acidic amino acid.Molecule includes two carboxyls, and chemical name is alpha-amido glutaric acid.Paddy ammonia
Acid is that inner Suo Xun has found for 1856, is clear crystal, there is delicate flavour, is slightly soluble in water, and is dissolved in hydrochloric acid solution, isoelectric point 3.22.
Largely it is present in grain protein, content is also more in animal brain.During the protein metabolism of glutamic acid in vivo
Critical role is accounted for, participates in many important chemical reactions in animal, plant and microorganism.
Glutamic acid is the highest acidic amino acid of content in mammal brain, participates in protein, the conjunction of polypeptide and aliphatic acid
Into；With adjusting internal ammonia level together with glutamine.
Important neurotransmitter of the glutamic acid as most synaptic excitations in mediating central nervous system, is related to intracerebral
Many important physiological functions.
It is all more complicated to be presently used for the manufacturing condition of extraction glutamic acid, reaction time length, production cost is high, right
It is low in the utilization rate of raw material.At the same time on being had not been reported using the technique of corn protein powder extraction glutamic acid.
The content of the invention
It is an object of the invention to provide a kind of method that glutamic acid is extracted from corn protein powder, to solve above-mentioned technology
Problem.
The present invention using following technical scheme in order to solve the above technical problems, realized：
A kind of method that glutamic acid is extracted from corn protein powder, comprises the following steps：
Step 1: the pretreatment of corn protein powder；
Step 2: the fermentation process of corn protein powder；
Step 3: the extraction purification of glutamic acid.
Preferably, the pretreatment of the corn protein powder of the step 1 includes：
（1）, remove corn protein powder in impurity, screening；
（2）, under the action of supersonic airstream head-on collision impact corn protein powder is ground into ultra-fine corn protein powder；
（3）, ultra-fine corn protein powder after being pulverized add dilute sulfuric acid and dilute hydrochloric acid solution, kept the temperature under conditions of 60-70 DEG C
5-8min is reacted, is put into baking oven, is dried to moisture less than 13%；
（4）, by after drying ultra-fine corn protein powder carry out supercritical carbon dioxide extracting removal of impurities, carry out secondary removal of impurities, extract
Condition is pressure 10MPa, 37 DEG C of extraction temperature, carbon dioxide flow 30L/h, and dynamic extraction 30min, carries out removal of impurities processing, is taken off
Go out peculiar smell and remaining chemical reagent and microorganism；
（5）, under 2-8 DEG C, vacuum condition, be dried, corn protein powder moisture be dried to less than 8%, obtains pre- place
Corn protein powder after reason.
Preferably, the fermentation process of the step 2 corn protein powder includes：It is 1.0 × 10 by viable count5-1.0×
106The zymocyte liquid access water content of cfu/mL is in the corn protein powder solution of 60-65%, is 37 DEG C in temperature, anaerobic condition
Lower fermentation 36-72h, obtains fermentation glutamic acid.
Preferably, the strain that the zymocyte liquid includes is：In Tianjin brevibacterium, Corynebacterium crenatum, Beijing corynebacterium
It is one or more.
Preferably, the extraction purification of the step 3 glutamic acid includes：
1）, by the fermentation glutamic acid obtained in step 2 carry out nanometer film for the first time filtering and supermembrane secondary filter, separation of solid and liquid；
2）, by the liquid isolated under conditions of 150-170 DEG C, be concentrated by evaporation, obtain glutamic acid particle；The bacterium that will be isolated
Body solid is ground, and the hydrochloric acid for adding 3moL/L carries out acidolysis, and the acidolysis 5-7h under conditions of 60-70 DEG C, then uses hydrogen-oxygen
Change sodium and be neutralized to pH equal to 7；
3）, into the solid isolated add solid masses 1% sodium chloride and solid masses 150% isopropanol, shaking
Bed rotating speed reacts 15-30min under conditions of being 500r/min, is maintained at 2-8 DEG C, in vacuum environment, glutamic acid is gradually analysed
Go out.
Preferably, the temperature of the cryogenic conditions is 2-8 DEG C.
The beneficial effects of the invention are as follows：
The present invention provides a kind of method that glutamic acid is extracted from corn protein powder, utilizes supercritical carbon dioxide extraction method pair
Ultra-fine corn protein powder is cleaned, is deodorized, is degerming, improves the quality of corn protein powder；By the use of isopropanol as precipitating reagent,
Under low-temperature vacuum environment, promote the precipitation of glutamic acid, improve the preparation efficiency of glutamic acid, and isopropanol is reusable, drop
Low cost；By the use of corn protein powder as raw materials for production, price is cheaper, substantially reduces production cost, while the present invention
Product purity is very high made from method, and the process conditions of production are relatively simple, are more suitable for use of large-scale production.
Embodiment
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention easy to understand, tie below
Specific embodiment is closed, the present invention is further explained, but following embodiments are only the preferred embodiment of the present invention, and not all.
Based on the embodiment in embodiment, those skilled in the art obtain other realities on the premise of creative work is not made
Example is applied, belongs to protection scope of the present invention.Experimental method in following embodiments, is conventional method unless otherwise specified,
The materials, reagents and the like used in the following examples, is commercially available unless otherwise specified.
Embodiment 1
A kind of method that glutamic acid is extracted from corn protein powder, comprises the following steps：
Step 1: the pretreatment of corn protein powder：
（1）, remove corn protein powder in impurity, screening；
（2）, under the action of supersonic airstream head-on collision impact corn protein powder is ground into ultra-fine corn protein powder；
（3）, ultra-fine corn protein powder after being pulverized add dilute sulfuric acid and dilute hydrochloric acid solution, the insulation reaction under conditions of 60 DEG C
5min, is put into baking oven, is dried to moisture less than 13%；
（4）, by after drying ultra-fine corn protein powder carry out supercritical carbon dioxide extracting removal of impurities, carry out secondary removal of impurities, extract
Condition is pressure 10MPa, 37 DEG C of extraction temperature, carbon dioxide flow 30L/h, and dynamic extraction 30min, carries out removal of impurities processing, is taken off
Go out peculiar smell and remaining chemical reagent and microorganism；
（5）, under 2-8 DEG C, vacuum condition, be dried, corn protein powder moisture be dried to less than 8%, obtains pre- place
Corn protein powder after reason.
Step 2: the fermentation process of corn protein powder：
It is 1.0 × 10 by Corynebacterium crenatum viable count5The corn protein powder that the zymocyte liquid access water content of cfu/mL is 60% is molten
It it is 37 DEG C in temperature in liquid, ferment 36h under anaerobic condition, obtains fermentation glutamic acid；
Step 3: the extraction purification of glutamic acid：
1）, by the fermentation glutamic acid obtained in step 2 carry out nanometer film for the first time filtering and supermembrane secondary filter, separation of solid and liquid；
2）, by the liquid isolated under conditions of 150-170 DEG C, be concentrated by evaporation, obtain glutamic acid particle；The bacterium that will be isolated
Body solid is ground, add 3moL/L hydrochloric acid carry out acidolysis, the acidolysis 5h under conditions of 60 DEG C, then with sodium hydroxide into
Row is neutralized to pH equal to 7；
3）, into the solid isolated add solid masses 1% sodium chloride and solid masses 150% isopropanol, shaking
Bed rotating speed reacts 15-30min under conditions of being 500r/min, is maintained at 2-8 DEG C, in vacuum environment, glutamic acid is gradually analysed
Go out.
Embodiment 2
A kind of method that glutamic acid is extracted from corn protein powder, comprises the following steps：
Step 1: the pretreatment of corn protein powder：
（1）, remove corn protein powder in impurity, screening；
（2）, under the action of supersonic airstream head-on collision impact corn protein powder is ground into ultra-fine corn protein powder；
（3）, ultra-fine corn protein powder after being pulverized add dilute sulfuric acid and dilute hydrochloric acid solution, the insulation reaction under conditions of 70 DEG C
8min, is put into baking oven, is dried to moisture less than 13%；
（4）, under 2-8 DEG C, vacuum condition, be dried, corn protein powder moisture be dried to less than 8%, obtains pre- place
Corn protein powder after reason.
Step 2: the fermentation process of corn protein powder：
It is 1.0 × 10 by Corynebacterium crenatum viable count6The zein that the zymocyte liquid access water content of cfu/mL is 60-65%
It it is 37 DEG C in temperature in powder solution, ferment 72h under anaerobic condition, obtains fermentation glutamic acid；
Step 3: the extraction purification of glutamic acid：
1）, by the fermentation glutamic acid obtained in step 2 carry out nanometer film for the first time filtering and supermembrane secondary filter, separation of solid and liquid；
2）, by the liquid isolated under conditions of 150-170 DEG C, be concentrated by evaporation, obtain glutamic acid particle；The bacterium that will be isolated
Body solid is ground, add 3moL/L hydrochloric acid carry out acidolysis, the acidolysis 7h under conditions of 70 DEG C, then with sodium hydroxide into
Row is neutralized to pH equal to 7；
3）, wiring solution-forming in the solid isolated centrifuged, take supernatant, be concentrated and dried, obtain the paddy of glutamic acid
Propylhomoserin finished product.
Embodiment 1 and 2 obtained result of embodiment are tested：
The corn protein powder of phase homogenous quantities produces glutamic acid, and the glutamic acid output quality of embodiment 1 is produced than the glutamic acid of embodiment 2
Mass more 10.2%；Glutamic acid purity prepared by embodiment 1 is higher by 5.7% than glutamic acid purity prepared by embodiment 2.
Basic principle, main feature and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
For personnel it should be appreciated that the present invention is not limited to the above embodiments, described in the above embodiment and specification is only the present invention
Preference, be not intended to limit the present invention, without departing from the spirit and scope of the present invention, the present invention also have it is various
Changes and improvements, these changes and improvements all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by institute
Attached claims and its equivalent thereof.
Claims (6)
- A kind of 1. method that glutamic acid is extracted from corn protein powder, it is characterised in that comprise the following steps：Step 1: the pretreatment of corn protein powder；Step 2: the fermentation process of corn protein powder；Step 3: the extraction of glutamic acid Purifying.
- 2. the method according to claim 1 that glutamic acid is extracted from corn protein powder, it is characterised in that：The step 1 The pretreatment of corn protein powder include：, remove corn protein powder in impurity, screening；, under the action of supersonic airstream head-on collision impact corn protein powder is ground into ultra-fine corn protein powder；, ultra-fine corn protein powder after being pulverized add dilute sulfuric acid and dilute hydrochloric acid solution, the insulation reaction under conditions of 60-70 DEG C 5-8min, is put into baking oven, is dried to moisture less than 13%；, by after drying ultra-fine corn protein powder carry out supercritical carbon dioxide extracting removal of impurities, carry out secondary removal of impurities, extraction conditions For pressure 10MPa, 37 DEG C of extraction temperature, carbon dioxide flow 30L/h, dynamic extraction 30min, removal of impurities processing is carried out, abjection is different Taste and remaining chemical reagent and microorganism；, under 2-8 DEG C, vacuum condition, be dried, corn protein powder moisture be dried to less than 8%, is pre-processed Corn protein powder afterwards.
- 3. the method according to claim 1 that glutamic acid is extracted from corn protein powder, it is characterised in that：The step 2 The fermentation process of corn protein powder includes：It is 1.0 × 10 by viable count5-1.0×106The zymocyte liquid access water content of cfu/mL To be 37 DEG C in temperature in the corn protein powder solution of 60-65%, ferment 36-72h under anaerobic condition, obtains fermentation glutamic acid.
- 4. the method according to claim 3 that glutamic acid is extracted from corn protein powder, it is characterised in that：The zymophyte The strain that liquid includes is：One or more in Tianjin brevibacterium, Corynebacterium crenatum, Beijing corynebacterium.
- 5. the method according to claim 1 that glutamic acid is extracted from corn protein powder, it is characterised in that：The step 3 The extraction purification of glutamic acid includes：1）, by the fermentation glutamic acid obtained in step 2 carry out nanometer film for the first time filtering and supermembrane secondary filter, separation of solid and liquid；2）, by the liquid isolated under conditions of 150-170 DEG C, be concentrated by evaporation, obtain glutamic acid particle；The bacterium that will be isolated Body solid is ground, and the hydrochloric acid for adding 3moL/L carries out acidolysis, and the acidolysis 5-7h under conditions of 60-70 DEG C, then uses hydrogen-oxygen Change sodium and be neutralized to pH equal to 7；3）, into the solid isolated add solid masses 1% sodium chloride and solid masses 150% isopropanol, shaking Bed rotating speed reacts 15-30min under conditions of being 500r/min, is maintained at 2-8 DEG C, in vacuum environment, glutamic acid is gradually analysed Go out.
- 6. the method according to claim 5 that glutamic acid is extracted from corn protein powder, it is characterised in that：The low temperature bar The temperature of part is 2-8 DEG C.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105950676A (en) * | 2016-07-16 | 2016-09-21 | 内蒙古阜丰生物科技有限公司 | Technology for preparing, separating and purifying polyglutamic acid |
| WO2017019125A1 (en) * | 2015-07-24 | 2017-02-02 | Synthetic Genomics, Inc. | A protein rich food ingredient from biomass and methods of production |
| CN106868068A (en) * | 2017-03-01 | 2017-06-20 | 中粮生化能源（龙江）有限公司 | A kind of utilization corn protein powder hydrolyzate substitutes the temperature sensitive type aminoglutaric acid fermentation production method of part soybean meal hydrolysate |
-
2017
- 2017-12-13 CN CN201711323865.1A patent/CN107988282A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017019125A1 (en) * | 2015-07-24 | 2017-02-02 | Synthetic Genomics, Inc. | A protein rich food ingredient from biomass and methods of production |
| CN105950676A (en) * | 2016-07-16 | 2016-09-21 | 内蒙古阜丰生物科技有限公司 | Technology for preparing, separating and purifying polyglutamic acid |
| CN106868068A (en) * | 2017-03-01 | 2017-06-20 | 中粮生化能源（龙江）有限公司 | A kind of utilization corn protein powder hydrolyzate substitutes the temperature sensitive type aminoglutaric acid fermentation production method of part soybean meal hydrolysate |
Non-Patent Citations (4)
| Title |
|---|
| 吕小妹等: "玉米综合深加工应用的研究进展", 《农产品加工》 * |
| 周清涛: "玉米蛋白粉改性技术及其理化特性的研究", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 李维锋等: "玉米蛋白粉二次开发及应用", 《掖食与油脂》 * |
| 鞠蕾等: "γ－聚谷氨酸的提取方法改进", 《现代化工》 * |
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Application publication date: 20180504 |