CN107988066A - Control system and control method - Google Patents
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- CN107988066A CN107988066A CN201610958047.8A CN201610958047A CN107988066A CN 107988066 A CN107988066 A CN 107988066A CN 201610958047 A CN201610958047 A CN 201610958047A CN 107988066 A CN107988066 A CN 107988066A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/34—Measuring or testing with condition measuring or sensing means, e.g. colony counters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M1/00—Apparatus for enzymology or microbiology
- C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q3/00—Condition responsive control processes
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05B—CONTROL OR REGULATING SYSTEMS IN GENERAL; FUNCTIONAL ELEMENTS OF SUCH SYSTEMS; MONITORING OR TESTING ARRANGEMENTS FOR SUCH SYSTEMS OR ELEMENTS
- G05B19/00—Programme-control systems
- G05B19/02—Programme-control systems electric
- G05B19/04—Programme control other than numerical control, i.e. in sequence controllers or logic controllers
Abstract
The present invention discloses a kind of control system and control method, for controlling imaging and Image Acquisition.Control system includes host computer, slave computer, driving platform, image collecting device and light source for carrying sample.Host computer is used to send initialization directive.Slave computer is used to send driving instruction according to initialization directive.Platform is driven to be used for the setting position for calculating each mobile example according to initialization directive, and according to driving instruction and setting position mobile example.When driving platform mobile example to setting position, source emissioning light is controlled to sample using slave computer so that sample excitation goes out detection light, and control image collecting device acquisition testing light to form view data.Image collecting device is used to view data being directly transferred to host computer.Above-mentioned control system, can reduce the data transmission times of host computer and slave computer, while view data is directly transferred to host computer, so can further shorten the response time between each component of control system, realize quick sequencing.
Description
Technical field
The present invention relates to gene sequencing field, more particularly to a kind of control system for being used to control imaging and Image Acquisition and
Control method.
Background technology
What is be currently known includes the instrument of imaging system, such as gene sequencer, and often single part passes through string respectively
Mouth and host computer compunlcation, are given a response by host computer computer according to motion state, and this process consumes substantial amounts of communication
Shake hands time and data transmission period, cause the low of imaging and data acquisition efficiency, and then influence the efficiency of gene sequencing.
The content of the invention
Embodiment of the present invention is intended at least solve one of technical problem existing in the prior art.For this reason, the present invention is real
The mode of applying provides a kind of control system and control method.
The control system of embodiment of the present invention, for controlling imaging and Image Acquisition, such as in gene sequencer
Imaging process communication control.The control system includes host computer, slave computer, the driving platform for carrying sample, figure
As harvester and light source；The host computer is used to send initialization directive；The slave computer is used to be referred to according to the initialization
Order sends driving instruction；The driving platform is used to calculate the setting position for moving the sample every time according to the initialization directive
Put, and the sample is moved according to the driving instruction and the setting position；Arrived when the driving platform moves the sample
During the setting position, the source emissioning light is controlled to the sample using the slave computer so that the sample excitation goes out inspection
Light is surveyed, and control described image harvester gathers the detection light to form view data.Described image harvester is used
In described image data are directly transferred to the host computer.
In this way, above-mentioned control system, the data transmission times of host computer and slave computer can be reduced, while view data is direct
Host computer is transmitted to, so can further shorten the response time between each component of control system, realizes quick sequencing.
In some embodiments, described image harvester is used to receive the initialization directive and according to described initial
Change instruction to open.
In some embodiments, the host computer by method wirelessly or non-wirelessly by the initialization directive send to
Described image harvester and the described image data for receiving the transmission of described image harvester.
In some embodiments, the slave computer includes input/output end port, and the input/output end port is used for defeated
Go out the first transistor-transistor logic level signal to control the source emissioning light, and control described image harvester to adopt
Collect the detection light.
In some embodiments, when controlling described image harvester to gather the detection light, when reaching the figure
As harvester the setting time for exposure after, the slave computer is used to control the light source to close.
In some embodiments, the slave computer includes input/output end port, and the input/output end port is used for defeated
Go out second transistor-transistor logic level signal to control the light source to close.
In some embodiments, after light source closing, the slave computer is used to send the driving instruction again
To the driving platform so that the sample is moved to next setting position to complete all institutes by the driving platform
State the collection of the corresponding described image data in setting position；Complete the corresponding described image data in all setting positions
During collection, the slave computer be used for send END instruction to the host computer with complete sequencing Image Acquisition.
In some embodiments, the driving instruction is pulse signal.
In some embodiments, described image harvester includes chasing after burnt module and object lens, the host computer be used for
It is described to chase after burnt module and send the initialization directive, it is described to chase after burnt module and be used for according to the initialization directive by control
Object lens movement is with when described image harvester gathers the detection light, it with the sample distance is constant to keep the object lens
's.
A kind of control method of embodiment of the present invention, for controlling imaging and Image Acquisition, such as controlling gene
The communication of the imaging process of sequenator, the control method include step：Host computer sends initialization directive；Slave computer is according to institute
State initialization directive and send driving instruction；Driving platform calculates the setting for moving the sample every time according to the initialization directive
Position, and the sample is moved according to the driving instruction and the setting position；When the driving platform moves the sample
During to the setting position, source emissioning light is controlled to the sample using the slave computer so that the sample excitation goes out detection
Light, and control detection light described in image acquisition device to form view data；Described image harvester is by the figure
As data are directly transferred to the host computer.
In this way, above-mentioned control method, the data transmission times of host computer and slave computer can be reduced, while view data is direct
Host computer is transmitted to, so can further shorten the response time in control method between each component, realizes quick sequencing.
In some embodiments, the control method further includes step：Described image harvester receives described initial
Change instruction and opened according to the initialization directive.
In some embodiments, the host computer by method wirelessly or non-wirelessly by the initialization directive send to
Described image harvester and the described image data for receiving the transmission of described image harvester.
In some embodiments, the slave computer includes input/output end port, and the control method includes step：Institute
Input/output end port output the first transistor-transistor logic level signal is stated to control the source emissioning light, and control institute
State detection light described in image acquisition device.
In some embodiments, the control method further includes step：In control described image harvester collection institute
When stating detection light, after the setting time for exposure of described image harvester is reached, the slave computer controls the light source to close.
In some embodiments, the slave computer includes input/output end port, and the slave computer controls the light source to close
The step of closing, including step：The input/output end port exports second transistor-transistor logic level signal to control
State light source closing.
In some embodiments, the control method further includes step：After light source closing, the slave computer is again
It is secondary to send the driving instruction to the driving platform so that the sample is moved to next described set by the driving platform
Positioning is put to complete the collection of the corresponding described image data in all setting positions；Complete all setting positions pair
During the collection for the described image data answered, the slave computer sends END instruction and is adopted to the host computer with completing the image of sequencing
Collection.
In some embodiments, described image harvester includes chasing after burnt module and object lens, and the control method is also wrapped
Include step：The host computer chases after burnt module and sends the initialization directive to described, described to chase after burnt module according to the initialization
Instruct by controlling the object lens movement when described image harvester gathers the detection light, to keep the object lens and institute
It is constant to state sample distance.
The additional aspect and advantage of embodiment of the present invention will be set forth in part in the description, partly by from following
Become obvious in description, or recognize by the practice of embodiment of the present invention.
Brief description of the drawings
The above-mentioned and/or additional aspect and advantage of embodiment of the present invention retouch embodiment from reference to accompanying drawings below
It will be apparent and be readily appreciated that in stating, wherein：
Fig. 1 is the module diagram for the control system that embodiment of the present invention provides；
Fig. 2 is the module diagram for the control system that embodiment of the present invention provides；
Fig. 3 is the flow diagram for the control method that embodiment of the present invention provides.
Main element symbol description：
Control system 10, host computer 12, slave computer 14, drives platform 16, image collecting device 17, camera 172, chases after burnt mould
Block 174, object lens 176, light source 18.
Embodiment
Embodiments of the present invention are described below in detail, the example of the embodiment is shown in the drawings, wherein from beginning
Same or similar element is represented to same or similar label eventually or there is same or like element.Below by ginseng
The embodiment for examining attached drawing description is exemplary, and is only used for explaining the present invention, and is not considered as limiting the invention.
In the description of the present invention, it is to be understood that term " first ", " second " are only used for description purpose, and cannot
It is interpreted as indicating or implies relative importance or imply the quantity of the technical characteristic indicated by indicating.Thus, define " the
One ", one or more feature can be expressed or be implicitly included to the feature of " second ".In description of the invention
In, " multiple " are meant that two or more, unless otherwise specifically defined.
In the description of the present invention, it is necessary to which explanation, unless otherwise clearly defined and limited, term " connection " should be done
It broadly understood, for example, it may be being fixedly connected or being detachably connected, or be integrally connected；Can mechanically connect,
Can also be electrically connected or can be in communication with each other；It can be directly connected, can also be indirectly connected by intermediary, Ke Yishi
The interaction relationship of connection or two elements inside two elements.For the ordinary skill in the art, can be with
The concrete meaning of above-mentioned term in the present invention is understood as the case may be.
Following disclosure provides many different embodiments or example is used for realizing the different structure of the present invention.In order to
Simplify disclosure of the invention, hereinafter to the components of specific examples and be set for describing.Certainly, they are merely examples, and
And it is not intended to limit the present invention.In addition, the present invention can in different examples repeat reference numerals and/or reference letter,
This repetition is for purposes of simplicity and clarity, itself not indicate between discussed various embodiments and/or setting
Relation.In addition, the present invention provides various specific techniques and material examples, but those of ordinary skill in the art can be with
Recognize the application of other techniques and/or the use of other materials.
Referring to Fig. 1, the control system 10 of embodiment of the present invention, for control imaging and Image Acquisition, such as
The communication control of imaging process in gene sequencer.Control system 10 includes host computer 12, slave computer 14, for carrying sample
Driving platform 16, image collecting device 17 and light source 18.
Host computer 12 is used to send initialization directive.Slave computer 14 is used to send driving instruction according to initialization directive.Drive
Moving platform 16 is used for the setting position that each mobile example is calculated according to initialization directive, and according to driving instruction and setting position
Mobile example.When driving 16 mobile example of platform to setting position, light source 18 is controlled to emit light into sample using slave computer 14
So that sample excitation goes out detection light, and control 17 acquisition testing light of image collecting device to form view data.Image Acquisition
Device 17 is used to view data being directly transferred to host computer 12.
In this way, above-mentioned control system 10, the data transmission times of host computer 12 and slave computer 14, while picture number can be reduced
It according to host computer 12 is directly transferred to, so can further shorten the response time between 10 each component of control system, realize quick
Sequencing.
Specifically, driving platform 16 carries sample, and driving platform 16 controls movement of the sample in gene sequencer, drives
Moving platform 16 includes position calculation unit, and the setting position of each mobile example is calculated with gene sequencing according to initialization directive
Process mobile example.Such as in high-flux sequence, once sequencing need to gather the sample image data of multiple setting positions, driving is flat
Platform 16 calculates the setting position of driving sample every time then according to initialization directive, with when receiving driving instruction, according to each
Sample is moved to image collecting device 17 by setting position can gather the region of image.It is preferred that driving platform 16 can realize XYZ
The movement of three direction of principal axis is with mobile example to setting position.
In embodiments of the present invention, image collecting device 17 includes camera 172 to convert optical signal into electric signal.
In one example, image collecting device 17 includes light path module and camera 172, and sample is positioned on driving platform 16, positioned at light
The thing side of road module, camera 172 are located at the image side of light path module.Light path module can be microscope.
In some embodiments, image collecting device 17 is used to receive initialization directive and be opened according to initialization directive
Open.
In this way, image collecting device 17 is in opening upon initialization, make the collection inspection of image collecting device 17
Survey the speed of light faster.
In some embodiments, initialization directive is sent to image and adopted by host computer 12 by method wirelessly or non-wirelessly
Acquisition means 17 and the view data for receiving the transmission of image collecting device 17.
In this way, realize the data transfer between host computer 12 and image collecting device 17.
Specifically, the data transfer mode between host computer 12 and image collecting device 17 can be that WLAN passes
Defeated or Bluetooth transmission, can also be universal serial bus transmission.Certainly, in other embodiments, it is not limited to above-mentioned
Transmission mode, can select suitable transmission mode according to the actual requirements.
In some embodiments, slave computer 14 includes input/output end port, and input/output end port is used to export first
Transistor-transistor logic level signal (ttl signal) with control light source 18 launch light, and control image collecting device 17 gather
Detection light.
In this way, slave computer 14 controls light source 18 and image collector by the first transistor-transistor logic level signal
17 are put, reduces the call duration time between slave computer 14 and light source 18 and image collecting device 17, it is further quick to obtain image,
Realize quick sequencing.
Specifically, in one example, light source 18 sends the laser of specific wavelength, and irradiating sample, makes the nucleosides in sample
The label that acid carries sends fluorescence, which gathers the fluorescence to form picture number as detection light, image collecting device 17
According to.
Further, transistor-transistor logic level signal transmission rate is Microsecond grade, is compared in correlation technique
Communicated by serial ports, transistor-transistor logic level signal makes slave computer 14 and light source 18 and image collecting device 17
Between realize high-speed traffic, reduce the call duration time between slave computer 14 and all parts, be advantageously implemented quick sequencing, and
The control system 10 of embodiment of the present invention completes a wheel sequencing, can complete a collection for setting position image, repeatedly weight
The shortening of accumulative call duration time is more notable after multiple.
In some embodiments, when controlling 17 acquisition testing light of image collecting device, when reaching image collecting device
After 17 setting time for exposure, slave computer 14 is used to control light source 18 to close.
In this way, slave computer 14 controls light source 18 to shine within the time for exposure of image collecting device 17, after end exposure
Close so that the image that image collecting device 17 gathers is apparent, and has saved the energy.
Specifically, in some embodiments, the time for exposure can be set in several ways, such as according to circumstances into pedestrian
To set, analogue exposure process is either carried out before sequencing and obtains the optimum time for exposure or conjunction is calculated by algorithm
Suitable exposure time values.Certainly, in other embodiments, the above method setting time for exposure is not limited to, can be in practical applications
The appropriate method setting time for exposure is selected according to actual conditions.
In some embodiments, slave computer 14 includes input/output end port, and input/output end port is used to export second
Transistor-transistor logic level signal is to control light source 18 to close.
In this way, slave computer 14 exports second transistor-transistor logic level signal by input/output end port closes light
Source 18, reduces the call duration time between slave computer 14 and light source 18, is advantageously implemented quick sequencing.
In some embodiments, after the closing of light source 18, it is flat to driving that slave computer 14 is used for transmission driving instruction again
Platform 16 is so that sample is moved to next setting position to complete the corresponding view data in all setting positions by driving platform 16
Collection；
When completing the collection of the corresponding view data in all setting positions, slave computer 14 is supreme for sending END instruction
Position machine 12 is with the Image Acquisition of completion sequencing.
Scheme in this way, the control system 10 of embodiment of the present invention carries out collection one by one to each setting position of mobile example
Picture, realizes the high sequencing of high throughput.
Specifically, the control system 10 of embodiment of the present invention, image collecting device 17 are connected with host computer 12, and image is adopted
Acquisition means 17 often collect the view data of a setting position, view data are transmitted to host computer 12, slave computer 14 is in light
After source 18 is closed, sending driving instruction to driving platform 16 makes driving platform 16 that sample is moved to next setting position, under
Position machine 14 is transmitted without waiting for view data and the time is sequenced with further shorten.
Further, in one example, in a control system 10, alleged driving platform 16 includes objective table
(stage), objective table, which includes, carries the chip of sample, and chip has multiple passages (Channel), gene sequencer into
, it is necessary to obtain the sample image data on one or more passages in picture and image acquisition process, and the sample on each passage
View data is made of multiple visuals field (Field of View, FOV).Usually, obtaining the time needed for a FOV includes：
Stage traveling times, stage call duration times, 172 time for exposure of camera, synchronous obtain 172 data of camera (i.e. camera 172 gather
The view data arrived) 172 call duration time of time and camera.And carried out into using the control system 10 of embodiment of the present invention
As the communication control of Image Acquisition, the acquisition for each FOV, can omit stage call duration times, 172 call duration time of camera with
And the synchronous time for obtaining 172 data of camera.
Further, in one example, it is intended to take pictures to the sample on multiple Channel on chip, sets
Each Channel obtains 300 FOV, sets stage shift positions to be controlled according to the FOV numbers of setting.Stage communicates
Time is arranged to 20ms, and 172 time for exposure of camera may be configured as 100ms, if being using the synchronous mode for obtaining 172 data of camera
Each 172 data transfer of camera needs 40ms every time to PC ends (referring to computer end)；And utilize any of the present invention or embodiment
Embodiment, stage call duration times are 0, and by the way of asynchronous acquisition 172 data of camera, it is only necessary to wait the passage
Last FOV completes to transmit again, i.e., transmission time is 40ms, you can omits 172 call duration time of camera and synchronous acquisition phase
The time of 172 data of machine.
In some embodiments, driving instruction is pulse signal.
In this way, slave computer 14 sends driving instruction to platform 16 is driven by the form of pulse signal, reduce slave computer
Call duration time between 14 and driving platform 16, is advantageously implemented quick sequencing.
Referring to Fig. 2, in some embodiments, image collecting device 17 includes chasing after burnt module 174 and object lens 176, on
Position machine 12 is used to, to the burnt transmission of module 174 initialization directive is chased after, chase after burnt module 174 and be used to pass through object lens according to initialization directive
176 movements are constant in 17 acquisition testing light of image collecting device, to keep object lens 176 and the distance of sample.
In this way, the setting position for needing to gather image as each on sample is when on same X/Y plane, by chasing after
Burnt module 174 adjusts the distance between object lens 176 and sample, image collecting device 17 is collected different X/Y plane setting positions
On picture rich in detail.
Specifically, in embodiment of the present invention, object lens 176 and the distance of sample are object distance.Host computer 12 refers to initialization
Order is sent to burnt module 174 is chased after, and is made to chase after burnt module 174 to start and is chased after burnt function automatically.
Burnt module 174 is chased after according to initialization directive, can control object lens 176 relative to the movement of sample so that 172 energy of camera
Enough into clearly sample image.The burnt function of lock is performed into burnt module 174 after clearly sample image, is chased after in definite camera 172,
That is when the sample position of required collection changes and causes object lens 176 and the distance of sample to change, burnt mould is chased after
Block 174 compensates variable quantity by controlling the movement of object lens 176 so that sample image formed by camera 172 remains clear.
Referring to Fig. 3, a kind of control method, for controlling imaging and Image Acquisition, such as in gene sequencer
The communication control of imaging process, control method include step：
S1：Host computer 12 sends initialization directive；
S2：Slave computer 14 sends driving instruction according to initialization directive；
S3：Platform 16 is driven to calculate the setting position of each mobile example according to initialization directive, and according to driving instruction
And setting position mobile example；
When driving 16 mobile example of platform to setting position, S4：Light source 18 is controlled to emit light into sample using slave computer 14
Product are so that sample excitation goes out detection light, and control 17 acquisition testing light of image collecting device to form view data；
S5：View data is directly transferred to host computer 12 by image collecting device 17.
In this way, above-mentioned control method, the data transmission times of host computer 12 and slave computer 14, while view data can be reduced
Host computer 12 is directly transferred to, so can further shorten the response time in control method between each component, realizes quick survey
Sequence.
It should be noted that the explanation of the above-mentioned embodiment to control system 10 is also applied for embodiment party of the present invention
The control method of formula, to avoid redundancy, is no longer developed in details herein.
In some embodiments, control method further includes step：Image collecting device 17 receives initialization directive and root
Opened according to initialization directive.
In this way, image collecting device 17 is in opening upon initialization, make the collection inspection of image collecting device 17
Survey the speed of light faster.
It should be noted that the explanation of the above-mentioned embodiment to control system 10 is also applied for embodiment party of the present invention
The control method of formula, to avoid redundancy, is no longer developed in details herein.
In some embodiments, initialization directive is sent to image and adopted by host computer 12 by method wirelessly or non-wirelessly
Acquisition means 17 and the view data for receiving the transmission of image collecting device 17.
In this way, realize the data transfer between host computer 12 and image collecting device 17.
It should be noted that the explanation of the above-mentioned embodiment to control system 10 is also applied for embodiment party of the present invention
The control method of formula, to avoid redundancy, is no longer developed in details herein.
In some embodiments, slave computer 14 includes input/output end port, and control method includes step：
Input/output end port exports the first transistor-transistor logic level signal (TTL signal) to control light source 18 to send out
Penetrate light, and control 17 acquisition testing light of image collecting device.
In this way, slave computer 14 controls light source 18 and image collector by the first transistor-transistor logic level signal
17 are put, reduces the call duration time between slave computer 14 and light source 18 and image collecting device 17, it is further quick to obtain image,
Realize quick sequencing.
It should be noted that the explanation of the above-mentioned embodiment to control system 10 is also applied for embodiment party of the present invention
The control method of formula, to avoid redundancy, is no longer developed in details herein.
In some embodiments, control method further includes step：
When controlling 17 acquisition testing light of image collecting device, when the setting time for exposure for reaching image collecting device 17
Afterwards, slave computer 14 controls light source 18 to close.
In this way, slave computer 14 controls light source 18 to shine within the time for exposure of image collecting device 17, after end exposure
Close so that the image that image collecting device 17 gathers is apparent, and has saved the energy.
It should be noted that the explanation of the above-mentioned embodiment to control system 10 is also applied for embodiment party of the present invention
The control method of formula, to avoid redundancy, is no longer developed in details herein.
In some embodiments, slave computer 14 includes input/output end port, and slave computer 14 controls the step that light source 18 is closed
Including step suddenly,：
Input/output end port exports second transistor-transistor logic level signal to control light source 18 to close.
In this way, slave computer 14 exports second transistor-transistor logic level signal by input/output end port closes light
Source 18, reduces the call duration time between slave computer 14 and light source 18, is advantageously implemented quick sequencing.
It should be noted that the explanation of the above-mentioned embodiment to control system 10 is also applied for embodiment party of the present invention
The control method of formula, to avoid redundancy, is no longer developed in details herein.
In some embodiments, control method further includes step：
After the closing of light source 18, S7：Slave computer 14 sends driving instruction to platform 16 is driven again, so that driving platform 16
Sample is moved to next setting position to complete the collection of the corresponding view data in all setting positions；
When completing the collection of the corresponding view data in all setting positions, S8：It is supreme that slave computer 14 sends END instruction
Position machine 12, to complete the Image Acquisition of sequencing.
Scheme in this way, the control system 10 of embodiment of the present invention carries out collection one by one to each setting position of mobile example
Picture, realizes the high sequencing of high throughput.
It should be noted that the explanation of the above-mentioned embodiment to control system 10 is also applied for embodiment party of the present invention
The control method of formula, to avoid redundancy, is no longer developed in details herein.
In some embodiments, image collecting device 17 includes chasing after burnt module 174 and object lens 176, and control method is also wrapped
Include step：
S6：Host computer 12 sends initialization directive to burnt module 174 is chased after, and chases after burnt module 174 and is passed through according to initialization directive
It is constant that control object lens 176, which are moved in 17 acquisition testing light of image collecting device, to keep object lens 176 and sample distance,.
In this way, the setting position for needing to gather image as each on sample is when on same X/Y plane, by chasing after
Burnt module 174 adjusts the distance between object lens 176 and sample, image collecting device 17 is collected different X/Y plane setting positions
On picture rich in detail.
It should be noted that the explanation of the above-mentioned embodiment to control system 10 is also applied for embodiment party of the present invention
The control method of formula, to avoid redundancy, is no longer developed in details herein.
In the description of this specification, reference term " embodiment ", " certain embodiments ", " schematically implementation
The description of mode ", " example ", " specific example " or " some examples " etc. means to combine embodiment or example describe it is specific
Feature, structure, material or feature are contained at least one embodiment or example of the present invention.In the present specification, it is right
The schematic representation of above-mentioned term is not necessarily referring to identical embodiment or example.Moreover, the specific features of description, knot
Structure, material or feature can combine in an appropriate manner in any one or more embodiments or example.
Although embodiments of the present invention have been shown and described above, it is to be understood that the above embodiment is
Exemplary, it is impossible to limitation of the present invention is interpreted as, those of ordinary skill in the art within the scope of the invention can be right
The above embodiment is changed, changes, replacing and modification.
Claims (8)
- A kind of 1. control system, for controlling imaging and Image Acquisition, it is characterised in that the control system includes：Host computer, the host computer are used to send initialization directive；Slave computer, the slave computer are used to send driving instruction according to the initialization directive；Platform is driven, the driving platform is used to carry sample, and the driving platform is used to calculate according to the initialization directive The setting position of the sample is moved every time, and the sample is moved according to the driving instruction and the setting position；Image collecting device and light source, when the driving platform moves the sample to the setting position, under described Position machine controls the source emissioning light to the sample so that the sample excitation goes out detection light, and control described image collection Device gathers the detection light to form view data；Described image harvester is used to described image data being directly transferred to the host computer.
- 2. control system as claimed in claim 1, it is characterised in that described image harvester is used to receive the initialization Instruct and opened according to the initialization directive.
- 3. control system as claimed in claim 2, it is characterised in that the host computer is by method wirelessly or non-wirelessly by institute State the described image data that initialization directive sent to described image harvester and received the transmission of described image harvester.
- 4. such as claim 1-3 any one of them control systems, it is characterised in that the slave computer includes input/output terminal Mouthful, the input/output end port is used to export the first transistor-transistor logic level signal to control the light source to launch Light, and control described image harvester gather the detection light；It is optional, when controlling described image harvester to gather the detection light, when reaching setting for described image harvester After determining the time for exposure, the slave computer is used to control the light source to close；Optional, the slave computer includes input/output end port, and the input/output end port is used to export second transistor-crystalline substance Body pipe logic level signal is to control the light source to close；Optional, after light source closing, the slave computer is used to send the driving instruction again to the driving platform Corresponded to so that the sample is moved to next setting position by the driving platform with completing all setting positions Described image data collection；When completing the collection of the corresponding described image data in all setting positions, the slave computer refers to for sending end Make to the host computer to complete the Image Acquisition of sequencing；Optional, the driving instruction is pulse signal；Optional, described image harvester includes chasing after burnt module and object lens, and the host computer is used to chase after burnt module hair to described Send the initialization directive, it is described to chase after burnt module and be used for according to the initialization directive by controlling the object lens movement with institute When stating detection light described in image acquisition device, the distance for keeping the object lens and the sample is constant.
- 5. a kind of control method, for gene sequencer, it is characterised in that the control method includes step：Host computer sends initialization directive；Slave computer sends driving instruction according to the initialization directive；Driving platform calculates the setting position for moving the sample every time according to the initialization directive, and is referred to according to the driving The sample is moved in order and the setting position；When the driving platform moves the sample to the setting position, using slave computer control source emissioning light extremely The sample is so that the sample excitation goes out detection light, and detection light described in control image acquisition device to form image Data；Described image data are directly transferred to the host computer by described image harvester.
- 6. control method as claimed in claim 5, it is characterised in that further include step：Described image harvester receives institute State initialization directive and opened according to the initialization directive.
- 7. control method as claimed in claim 6, it is characterised in that the host computer is by method wirelessly or non-wirelessly by institute State the described image data that initialization directive sent to described image harvester and received the transmission of described image harvester.
- 8. such as claim 5-7 any one of them control methods, it is characterised in that the slave computer includes input/output terminal Mouthful, the control method includes step：The input/output end port exports the first transistor-transistor logic level signal to control the source emissioning light, and Described image harvester is controlled to gather the detection light；Optional, the control method, further includes step：When controlling described image harvester to gather the detection light, when the setting for reaching described image harvester exposes Between after, the slave computer controls the light source to close；It is optional, the step of slave computer includes input/output end port, and the slave computer controls the light source closing, including Step：The input/output end port exports second transistor-transistor logic level signal to control the light source to close；Optional, the control method further includes step：After light source closing, the slave computer sends the driving instruction to the driving platform so that the driving again The sample is moved to next setting position to complete the corresponding described image number in all setting positions by platform According to collection；When completing the collection of the corresponding described image data in all setting positions, the slave computer sends END instruction extremely The host computer with complete sequencing Image Acquisition；Optional, described image harvester includes chasing after burnt module and object lens, and the control method further includes step：The host computer chases after burnt module and sends the initialization directive to described, described to chase after burnt module according to the initialization directive By control the object lens movement with described image harvester gather the detection light when, keep the object lens and the sample The distance of product is constant.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610958047.8A CN107988066A (en) | 2016-10-27 | 2016-10-27 | Control system and control method |
| PCT/CN2017/085179 WO2018076670A1 (en) | 2016-10-27 | 2017-05-19 | Control system and control method |
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| CN109239071A (en) * | 2018-11-20 | 2019-01-18 | 帝麦克斯(苏州)医疗科技有限公司 | Pathological section scanner control method and pathological section scanner |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111912993A (en) * | 2019-05-07 | 2020-11-10 | 旭月(北京)科技有限公司 | High-throughput automatic rapid non-damage micrometering system and method |
| CN114462004B (en) * | 2022-02-08 | 2023-08-22 | 无锡盛景微电子股份有限公司 | Method and system for automatically detecting lower-level machine body information in master-slave network |
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| CN105629780A (en) * | 2014-12-01 | 2016-06-01 | 深圳华大基因研究院 | Gene sequencing instrument, control device and method of gene sequencing instrument |
| CN105861293A (en) * | 2016-04-06 | 2016-08-17 | 深圳市瀚海基因生物科技有限公司 | Single-molecule gene sequencer |
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| CN102703314B (en) * | 2012-05-24 | 2013-09-04 | 中国科学院北京基因组研究所 | Control system for DNA (Deoxyribose Nucleic Acid) sequencer |
| US10851414B2 (en) * | 2013-10-18 | 2020-12-01 | Good Start Genetics, Inc. | Methods for determining carrier status |
| CN104297230A (en) * | 2014-09-16 | 2015-01-21 | 北京九州泰康生物科技有限责任公司 | Portable type biochip detection device |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105629780A (en) * | 2014-12-01 | 2016-06-01 | 深圳华大基因研究院 | Gene sequencing instrument, control device and method of gene sequencing instrument |
| CN105861293A (en) * | 2016-04-06 | 2016-08-17 | 深圳市瀚海基因生物科技有限公司 | Single-molecule gene sequencer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109239071A (en) * | 2018-11-20 | 2019-01-18 | 帝麦克斯(苏州)医疗科技有限公司 | Pathological section scanner control method and pathological section scanner |
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