CN107988059B - Self-sterilization type microorganism bacterium culture, reproduction and inoculation instrument - Google Patents

Self-sterilization type microorganism bacterium culture, reproduction and inoculation instrument Download PDF

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Publication number
CN107988059B
CN107988059B CN201810057759.1A CN201810057759A CN107988059B CN 107988059 B CN107988059 B CN 107988059B CN 201810057759 A CN201810057759 A CN 201810057759A CN 107988059 B CN107988059 B CN 107988059B
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inoculation
shell
rod
positive
module
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CN107988059A (en
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李长寿
吴良科
董志武
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Zhongnong Fude Testing Technology (Wuhan) Co.,Ltd.
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Zhongnong Fude Testing Technology Wuhan Co ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
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  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
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  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Abstract

The invention belongs to the technical field of microbial culture, and particularly relates to a self-sterilization microbial bacteria culture propagation inoculation instrument which comprises a shell, an inoculation rod, a rotary module, a sterilization module and a power supply module, wherein the inoculation rod is arranged on the shell; one end of the shell is provided with a notch; the inside of the notch is rotatably provided with an inoculation rod; both ends of the inoculation rod can be inoculated; the inoculation rod is rotated through the rotation module, and different end heads are switched for inoculation; the rotary module comprises a rotating shaft, a ratchet wheel, a pressing rod, a pawl and a return spring; the sterilization module comprises a positive contact switch, a positive wiring terminal, a negative contact switch, a negative wiring terminal and a heating wire; the power supply module supplies electric energy for the cooperation sterilization module; the inoculation rod is rotated through the rotation module, and different end heads are switched for inoculation; the end of the inoculation rod transferred into the shell is communicated with a circuit, and the power supply module is used for heating the sterilization module to realize bacterial killing. The invention can inoculate various bacteria, kill residual bacteria in time and improve the experimental efficiency.

Description

Self-sterilization type microorganism bacterium culture, reproduction and inoculation instrument
Technical Field
The invention belongs to the technical field of microbial culture, and particularly relates to a self-sterilization microbial bacteria culture propagation inoculation instrument.
Background
At present, in an analysis experiment of microorganisms such as a clinical bacterial analysis, it is necessary to inoculate a bacterial solution on the surface of a culture dish and then perform a scientific experiment using observation and analysis equipment.
In microbial research, a culture medium inoculator is an essential tool. However, there are two types, one being an inoculating loop: the existing inoculating loop comprises a nickel-chromium wire, a metal handle and an insulating handle, wherein a screw opening is formed in the nickel-chromium wire; the other is a glass spatula (also called a coating rod), which is a triangular coating rod regardless of glass materials or metal materials, and comprises a triangular spatula area and a handle, wherein the included angle between the handle and the plane of the triangular spatula area is 120-140 degrees. The prior inoculating loop and coating rod for inoculation have the following disadvantages: after inoculation, a new inoculation tool needs to be replaced to prevent cross contamination, multiple times of inoculation cannot be carried out by using one tool, and the efficiency is low; residual bacteria inoculated last time need to be killed before different bacteria are inoculated, and additional sterilization operation is needed, so that the working efficiency is influenced.
Disclosure of Invention
In order to make up the defects of the prior art, the self-sterilization microbial bacteria culture and propagation inoculating instrument provided by the invention can be used for inoculating various bacteria, killing residual bacteria in time and improving the experimental efficiency.
The invention relates to a self-sterilization microbial bacteria culture propagation inoculation instrument, which comprises a shell, an inoculation rod, a rotation module, a sterilization module and a power supply module, wherein the inoculation rod is arranged on the shell; one end of the shell is provided with a notch; the inoculation rod is rotatably arranged in the notch; both ends of the inoculation rod can be inoculated; the inoculation rod is rotated through the rotation module, and different end heads are switched for inoculation; the rotary module is arranged in the shell; the sterilization module is arranged in one end of the shell; the power supply module supplies electric energy to the sterilization module and is arranged in one end of the shell; the inoculation rod is rotated through the rotation module, and different end heads are switched for inoculation; the end of the inoculation rod transferred into the shell is communicated with a circuit, and the power supply module is used for heating the sterilization module to realize bacterial killing.
The rotary module comprises a rotating shaft, a ratchet wheel, a pressing rod, a pawl and a return spring; the rotating shaft is rotatably arranged in the middle of the shell and is fixedly connected to the middle of the inoculating rod, and one end of the rotating shaft is fixedly connected with the ratchet wheel; the ratchet wheel is rotatably arranged in the shell; the pressing rod is arranged inside the shell; the ratchet wheel is controlled to rotate by a pawl arranged on the pressing rod; one end of the pressing rod extends out of the shell, and the end head of the pressing rod is provided with a pressing block; the middle part of the cavity of the shell body provided with the pressing rod is provided with a step; a boss is arranged in the middle of the pressing plate; one end of the boss is provided with a return spring; the reset spring is used for resetting after being pressed by the pressing rod; a pawl arranged on the pressing rod drives a ratchet wheel to rotate for 180 degrees; the inoculation rod fixedly connected with the ratchet wheel rotates for a certain degree, so that the inoculation end head is switched.
The power supply module comprises a switch, a storage battery, a positive plate and a negative plate; the switch is arranged on one side of the shell; the storage battery is arranged at one end inside the shell; the storage battery is connected with the positive plate and the negative plate; the positive plate is arranged on one side of the notch of the shell; the negative plate is arranged on the other side of the notch of the shell.
Preferably, the sterilization module comprises a positive contact switch, a positive terminal, a negative contact switch, a negative terminal and a heating wire; notches are formed in the two ends of the inoculation rod; a positive electrode contact switch is arranged on one side of the inoculation rod; the positive contact switch is connected with a positive post; the positive terminal is arranged in the wiring rod and is positioned in the column on one side of the notch; the negative contact switch is connected with the negative post; the negative terminal is arranged in the wiring rod and is positioned in the column on the other side of the notch; a heating wire is connected between the positive terminal and the negative terminal, and after the inoculation rod rotates, the positive contact switch contacts the positive plate; the negative contact switch contacts the negative plate and is communicated with the circuit, so that the heating of the heating wire is realized, and the killing of bacteria is realized.
Preferably, liquid storage tanks are arranged at the joints of the positive terminal, the negative terminal and the heating wire; heating plates are arranged on two sides of the liquid storage tank, after the inoculation rod rotates, a part of liquid remaining at the end head flows into the liquid storage tank, and bacteria are killed through the arranged heating plates.
Preferably, the heating wire is a tungsten wire, and has high melting point, high resistivity and good strength.
Preferably, the positive contact switch and the negative contact switch both comprise a plastic base piece, a contact block, an upper limit stud, a lower limit stud, a spring and an elastic conducting ring; plastic seat parts are arranged on two sides of the inoculation rod; the plastic seat piece is internally matched and connected with the contact block; the contact block is used for contacting and connecting the positive plate or the negative plate; an upper limiting stud is arranged at one end of the contact block; the bottom of the plastic seat part is provided with a lower limiting stud; a spring is screwed between the upper limit stud and the lower limit stud; the periphery of the spring is sleeved with an elastic conductive ring; one side of the elastic conducting ring penetrates through the plastic seat piece and is connected to the positive terminal or the negative terminal, and after the inoculation rod rotates for a circle, the contact block of the positive contact switch or the negative contact switch is extruded and then contacts the positive plate or the negative plate; the positive terminal, the negative terminal and the heating wire are communicated through the elastic conducting ring; the function of heating sterilization is realized.
The invention has the following beneficial effects:
1. according to the self-sterilization type microbial bacteria culture propagation inoculation instrument, the rotation of the inoculation rod is controlled by the rotation module, different end heads are switched for inoculation, and the experiment efficiency is improved; and utilize the sterilization module after switching, realize the sterilization effect of inoculation end, need not additionally to carry out sterilization work, reduce experimenter work load.
2. According to the self-sterilization type microbial bacteria culture propagation inoculation instrument, the rotation of the inoculation rod is realized by utilizing the pawl and the ratchet wheel arranged on the rotary module, the switching of the inoculation end is realized, a new inoculation tool is not required to be replaced, and the working efficiency of experimenters is improved.
3. According to the self-sterilization microbial bacteria culture propagation inoculation instrument, after an inoculation rod rotates for one circle, a positive electrode contact switch is communicated with a positive plate, and a negative electrode contact switch is communicated with a negative plate; the heating of the electric heating wire is controlled through the switch, so that the heating and sterilizing functions are realized, and the device is simple and efficient.
4. According to the self-sterilization microbial bacteria culture propagation inoculation instrument, the heating wires are made of tungsten wires, so that the self-sterilization microbial bacteria culture propagation inoculation instrument is high in melting point, large in resistivity, good in strength, quick in heating and capable of improving sterilization efficiency.
5. According to the self-sterilization type microbial bacteria culture propagation inoculation instrument, the liquid storage tank and the heating plate are arranged, so that the backflow of the attached bacterial liquid is prevented, the sterilization is realized in all directions, and the sterilization is more thorough.
Drawings
The invention will be further explained with reference to the drawings.
FIG. 1 is a front view of the present invention;
FIG. 2 is a cross-sectional view A-A of FIG. 1;
FIG. 3 is a cross-sectional view C-C of FIG. 1;
FIG. 4 is a cross-sectional view B-B of FIG. 1;
in the figure: the device comprises a shell 1, an inoculation rod 2, a liquid storage tank 21, a heating plate 22, a rotary module 3, a rotating shaft 31, a ratchet wheel 32, a pressing rod 33, a boss 331, a pawl 34, a return spring 35, a sterilization module 4, a positive contact switch 41, a plastic seat piece 411, a contact block 412, an upper limiting stud 413, a lower limiting stud 414, a spring 415, an elastic conducting ring 416, a positive terminal 42, a negative contact switch 43, a negative terminal 44, a heating wire 45, a power supply module 5, a switch 51, a storage battery 52, a positive plate 53 and a negative plate 54.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
As shown in fig. 1 to 4, the self-sterilizing microbial bacteria culture propagation inoculator comprises a housing 1, an inoculating rod 2, a rotary module 3, a sterilizing module 4 and a power supply module 5; one end of the shell 1 is provided with a notch; the inoculation rod 2 is rotatably arranged in the notch; both ends of the inoculation rod 2 can be inoculated; the inoculation rod 2 is rotated through the rotation module 3, and different end heads are switched for inoculation; the rotary module 3 is arranged inside the shell 1; the sterilization module 4 is arranged inside one end of the shell 1; the power supply module 5 supplies electric energy to the sterilization module 4 and is arranged in one end of the shell 1; the inoculation rod 2 is rotated through the rotation module 3, and different end heads are switched for inoculation; the end of the inoculation rod 2 transferred into the shell 1 is communicated with a circuit, and the power supply module 5 is used for heating the sterilization module to realize the sterilization of bacteria.
The rotation module 3 comprises a rotating shaft 31, a ratchet 32, a pressing rod 33, a pawl 34 and a return spring 35; the rotating shaft 31 is rotatably arranged in the middle of the shell 1 and is fixedly connected to the middle of the inoculation rod 2, and one end of the rotating shaft 31 is fixedly connected with the ratchet wheel 32; the ratchet wheel 32 is rotatably arranged inside the shell 1; the pressing rod 33 is installed inside the shell; the ratchet wheel 32 is controlled to rotate by a pawl 34 arranged on a pressing rod 33; one end of the pressing rod 33 extends out of the shell 1, and the end head of the pressing rod is provided with a pressing block; the middle part of the cavity of the shell 1 for installing the pressing rod 33 is provided with a step; a boss 331 is arranged in the middle of the pressing plate 33; one end of the boss 331 is provided with a return spring 35; the return spring 35 is used for returning after being pressed by the pressing rod 33; the ratchet 32 is driven to rotate 180 degrees by the pawl 34 arranged on the pressing rod 33; the inoculation rod 2 fixedly connected with the ratchet wheel 32 rotates 180 degrees, and the inoculation end head is switched.
The power supply module 5 comprises a switch 51, a storage battery 52, a positive plate 53 and a negative plate 54; the switch 51 is arranged on one side of the shell 1; the storage battery 52 is arranged at one end inside the shell 1; the battery 52 is connected with a positive plate 53 and a negative plate 54; the positive plate 53 is arranged on one side of the notch of the shell 1; the negative plate 54 is disposed on the other side of the notch of the case 1.
As an embodiment of the present invention, the sterilization module 4 includes a positive contact switch 41, a positive post 42, a negative contact switch 43, a negative post 44, and a heating wire 45; notches are formed in the two ends of the inoculation rod 2; one side of the inoculation rod 2 is provided with an anode contact switch 41; the positive contact switch 41 is connected with a positive terminal 42; the positive terminal 42 is arranged in the wiring rod 2 and is positioned in the column on one side of the notch; the negative contact switch 43 is connected with a negative terminal 44; the negative terminal 44 is arranged inside the junction pole 2 and is positioned in the column on the other side of the notch; a heating wire 45 is connected between the positive terminal 42 and the negative terminal 44, and after the inoculation rod 2 rotates, the positive contact switch 41 contacts the positive plate 53; the negative contact switch 43 contacts the negative plate 54 to connect the circuit, so that the heating wire 45 is heated, and bacteria are killed.
As an embodiment of the invention, a liquid storage tank 21 is arranged at the joint of the positive terminal 42, the negative terminal 44 and the heating wire 45; heating plates 22 are arranged on two sides of the liquid storage tank 21, and after the inoculation rod 2 rotates, a part of liquid remaining at the end head flows into the liquid storage tank 21, and bacteria are killed through the arranged heating plates 22.
In one embodiment of the present invention, the heating wire 45 is a tungsten wire, which has a high melting point, a high resistivity, and a high strength.
As an embodiment of the present invention, each of the positive contact switch 41 and the negative contact switch 43 includes a plastic seat 411, a contact block 412, an upper limit stud 413, a lower limit stud 414, a spring 415, and an elastic conductive ring 416; the two sides of the inoculation rod 2 are provided with plastic seat parts 411; the plastic seat 411 is internally matched and connected with a contact block 412; the contact block 412 is used for contacting and connecting the positive plate 53 or the negative plate 54; an upper limit stud 413 is arranged at one end of the contact block 412; the bottom of the plastic seat piece 411 is provided with a lower limit stud 414; a spring 415 is screwed between the upper limit stud 413 and the lower limit stud 414; the elastic conductive ring 416 is sleeved on the outer periphery of the spring 415; one side of the elastic conducting ring 416 penetrates through the plastic seat piece 411 and is connected to the positive terminal 42 or the negative terminal 44, and after the inoculation rod 2 rotates for one circle, the contact block 412 of the positive contact switch 41 or the negative contact switch 43 is pressed to contact the positive plate 53 or the negative plate 54; the positive terminal 42, the negative terminal 44 and the heating wire 45 are communicated through the elastic conducting ring 416; the function of heating sterilization is realized.
When the device works, an operator firstly utilizes the end of the inoculation rod 2 for inoculation of bacteria; after one kind of inoculation is completed, the inoculation end needs to be replaced, at the moment, the rotary module 3 can be used for rotating the inoculation rod 2, namely, the pawl 34 arranged on the pressing rod 33 is used for driving the ratchet wheel 32 to rotate 180 degrees; the inoculation rod 2 fixedly connected with the ratchet 32 rotates 180 degrees, so that the inoculation end head is switched; if the inoculated end needs to be sterilized, the contact block 412 of the positive contact switch 41 or the negative contact switch 43 is pressed to contact the positive plate 53 or the negative plate 54 when the inoculation rod 2 rotates; the positive terminal 42, the negative terminal 44 and the heating wire 45 are communicated through the elastic conducting ring 416, and the switch 51 is only needed to be pressed to communicate the circuit, so that the heating wire 45 generates high temperature, and bacteria are killed; the rotary module 3 rotates the inoculation rod 2 to realize the circular operation, and the inoculation efficiency is improved.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims (1)

1. The utility model provides a self-sterilizer microorganism bacterial culture breeds inoculation appearance which characterized in that: comprises a shell (1), an inoculation rod (2), a rotary module (3), a sterilization module (4) and a power supply module (5); one end of the shell (1) is provided with a notch; the inoculation rod (2) is rotatably arranged in the notch; the inoculation rod (2) is rotated through the rotary module (3); the rotary module (3) is arranged in the shell (1); the sterilization module (4) is arranged in one end of the shell (1); the power supply module (5) provides electric energy for the sterilization module (4) and is arranged in one end of the shell (1); wherein the content of the first and second substances,
the rotary module (3) comprises a rotating shaft (31), a ratchet wheel (32), a pressing rod (33), a pawl (34) and a return spring (35); the rotating shaft (31) is rotatably arranged in the middle of the shell (1) and is fixedly connected to the middle of the inoculation rod (2), and one end of the rotating shaft (31) is fixedly connected with the ratchet wheel (32); the ratchet wheel (32) is rotatably arranged in the shell (1); the pressing rod (33) is arranged inside the shell; the ratchet wheel (32) is controlled to rotate by a pawl (34) arranged on a pressing rod (33); one end of the pressing rod (33) extends out of the shell (1), and the end head of the pressing rod is provided with a pressing block; a step is arranged in the middle of a cavity of the shell (1) for installing the pressing rod (33); a boss (331) is arranged in the middle of the pressing plate (33); one end of the boss (331) is provided with a return spring (35); the return spring (35) is used for returning after being pressed by the pressing rod (33);
the power supply module (5) comprises a switch (51), a storage battery (52), a positive plate (53) and a negative plate (54); the switch (51) is arranged on one side of the shell (1); the storage battery (52) is arranged at one end inside the shell (1); the storage battery (52) is connected with a positive plate (53) and a negative plate (54); the positive plate (53) is arranged on one side of the notch of the shell (1); the negative plate (54) is arranged on the other side of the notch of the shell (1);
the sterilization module (4) comprises a positive contact switch (41), a positive wiring terminal (42), a negative contact switch (43), a negative wiring terminal (44) and a heating wire (45); notches are formed in the two ends of the inoculation rod (2); a positive contact switch (41) is arranged on one side of the inoculation rod (2); the positive contact switch (41) is connected with a positive terminal (42); the positive terminal (42) is arranged in the wiring rod (2) and is positioned in the column on one side of the notch; the negative contact switch (43) is connected with a negative terminal (44); the negative terminal (44) is arranged in the wiring rod (2) and is positioned in the column on the other side of the notch; a heating wire (45) is connected between the positive terminal (42) and the negative terminal (44);
liquid storage tanks (21) are arranged at the joints of the positive terminal (42), the negative terminal (44) and the heating wire (45); heating plates (22) are arranged on two sides of the liquid storage tank (21);
the heating wire (45) is a tungsten wire;
the positive contact switch (41) and the negative contact switch (43) respectively comprise a plastic seat piece (411), a contact block (412), an upper limiting stud (413), a lower limiting stud (414), a spring (415) and an elastic conducting ring (416); plastic seat parts (411) are arranged on two sides of the inoculation rod (2); the plastic seat piece (411) is internally matched and connected with a contact block (412); the contact block (412) is used for being in contact connection with the positive plate (53) or the negative plate (54); an upper limiting stud (413) is arranged at one end of the contact block (412); the bottom of the plastic seat piece (411) is provided with a lower limiting stud (414); a spring (415) is screwed between the upper limit stud (413) and the lower limit stud (414); the outer periphery of the spring (415) is sleeved with an elastic conductive ring (416); one side of the elastic conducting ring (416) penetrates through the plastic seat piece (411) and is connected to the positive terminal (42) or the negative terminal (44).
CN201810057759.1A 2018-01-22 2018-01-22 Self-sterilization type microorganism bacterium culture, reproduction and inoculation instrument Active CN107988059B (en)

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Publication number Priority date Publication date Assignee Title
CN108165477B (en) * 2018-03-08 2023-09-26 湖南水口山有色金属集团有限公司 Quick sterilization device of microorganism inoculation instrument
CN114766360A (en) * 2022-04-22 2022-07-22 南通大学 Plant stem segment explant inoculation device

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CN2768177Y (en) * 2005-02-02 2006-03-29 梁林坤 Rotary switch
CN201124667Y (en) * 2007-12-03 2008-10-01 华东师范大学附属杨行中学 Multifunctional pen
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