CN107988059A - It is a kind of to breed inoculation instrument from the culture of disinfection type microbial bacterial - Google Patents

It is a kind of to breed inoculation instrument from the culture of disinfection type microbial bacterial Download PDF

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Publication number
CN107988059A
CN107988059A CN201810057759.1A CN201810057759A CN107988059A CN 107988059 A CN107988059 A CN 107988059A CN 201810057759 A CN201810057759 A CN 201810057759A CN 107988059 A CN107988059 A CN 107988059A
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China
Prior art keywords
inoculation
module
housing
bar
cathode contact
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CN201810057759.1A
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CN107988059B (en
Inventor
李长寿
吴良科
董志武
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Zhongnong Fude Testing Technology Wuhan Co ltd
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Individual
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Apparatus For Disinfection Or Sterilisation (AREA)

Abstract

It is specifically a kind of to breed inoculation instrument, including housing, inoculation bar, revolution module, sterilization module and power supply module from the culture of disinfection type microbial bacterial the invention belongs to field of microbial culture technology;Shell one end is equipped with notch;Notch inner rotation is installed with inoculation bar;Inoculation bar both ends can be inoculated with;Inoculation bar realizes rotation by turning round module, switches different termination inoculations;Revolution module includes shaft, ratchet, pressing lever, pawl and resetting spring;Sterilization module includes cathode contact-making switch, positive terminal, cathode contact switch, negative terminal and heater strip;Power supply module provides electric energy for cooperation sterilization module;Inoculation bar realizes rotation by turning round module, switches different termination inoculations;The inoculation rod end head of enclosure interior is transferred to, connection circuit, using power supply module heat sterilization module, realizes killing bacteria.The present invention can carry out various bacteria inoculation, and can kill remaining bacteria in time, improve conventional efficient.

Description

It is a kind of to breed inoculation instrument from the culture of disinfection type microbial bacterial
Technical field
It is specifically a kind of to be bred from the culture of disinfection type microbial bacterial the invention belongs to field of microbial culture technology It is inoculated with instrument.
Background technology
At present, it is necessary to which bacterium solution is seeded in culture dish in the analysis experiment to microorganism such as clinical bacterial analysis On surface, scientific experiment then is carried out to it with observation, analytical equipment etc..
In microbe research, culture medium microbionation device is essential instrument.But have two kinds at present, Yi Zhongshi Oese:Existing oese includes nickel filament, metal handle, insulation handle three parts, has a screw socket on nickel filament;Another It is glass spatula (being called spreading rod), whether the either metal material of glass material is triangle spreading rod, including Angle between plane where triangle spatula area and handle, handle and triangle spatula area is 120 ° -140 °.It is existing to be used to be inoculated with Oese and spreading rod have the disadvantages that:Need to need replacing new inoculating tool after inoculation, with anti-cross-contamination, it is impossible to Repeatedly it is inoculated with using an instrument, its is less efficient;Need the residual for killing last time inoculation thin before different bacterium inoculation Bacterium influences work efficiency, it is necessary to additionally carry out disinfecting action.
The content of the invention
In order to make up for the deficiencies of the prior art, a kind of bred from the culture of disinfection type microbial bacterial proposed by the present invention is inoculated with Instrument, can carry out various bacteria inoculation, and can kill remaining bacteria in time, improve conventional efficient.
A kind of bred from the culture of disinfection type microbial bacterial of the present invention is inoculated with instrument, including housing, inoculation bar, revolution Module, sterilization module and power supply module;The shell one end is equipped with notch;The notch inner rotation is installed with inoculation bar;Institute Stating inoculation bar both ends can be inoculated with;The inoculation bar realizes rotation by turning round module, switches different termination inoculations;Described time Revolving die block is installed inside housings;The sterilization module is installed on inside shell one end;The power supply module carries for sterilization module For electric energy, and inside shell one end;The inoculation bar realizes rotation by turning round module, switches different terminations and connects Kind;The inoculation rod end head of enclosure interior is transferred to, connection circuit, using power supply module heat sterilization module, realizes killing bacteria.
The revolution module includes shaft, ratchet, pressing lever, pawl and resetting spring;The shaft is rotatably installed in shell In the middle part of body, and it is fixedly connected in the middle part of inoculation bar, described shaft one end is fixedly connected ratchet;The pawl wheel is installed on shell Internal portion;The pressing lever installation is inside housings;The ratchet controls rotation by the pawl set on pressing lever;It is described to press Housing is stretched out in compression bar one end, and is equipped with briquetting in termination;Step is equipped with the middle part of the cavity of the housing installation pressing lever;It is described Boss is equipped with the middle part of pressing plate;Described boss one end is equipped with resetting spring;The resetting spring resets after being used for pressing lever pressing; The pawl driving pawl set using pressing lever rotates 180 °;The inoculation bar that ratchet is fixedly connected realizes rotation ° rotation, realizes It is inoculated with termination switching.
The power supply module includes switch, storage battery, positive plate and negative plate;The switch is installed on housing side;Institute State storage battery installation one end inside housings;The storage battery is connected to positive plate and negative plate;The positive plate is located at housing Notch side;The negative plate is located at housing notch opposite side.
Preferably, the sterilization module includes cathode contact-making switch, positive terminal, cathode contact switch, negative terminal Column and heater strip;The inoculation bar both ends are equipped with notch;The inoculation bar side is equipped with cathode contact-making switch;The cathode connects Tactile switch is connected to positive terminal;The positive terminal is installed on inside junction pole, and in the cylinder of notch side;Institute State cathode contact switch and be connected to negative terminal;The negative terminal is installed on inside junction pole, and another positioned at notch In lateral column body;Heater strip is connected between the positive terminal and negative terminal, after inoculation bar rotation, cathode contact-making switch Contact positive plate;Cathode contact switch contact negative plate, connection circuit, realizes that heater strip heats, realizes the killing of bacterium.
Preferably, the positive terminal, negative terminal and heater strip junction are equipped with reservoir;The reservoir Both sides are equipped with heating plate, and after inoculation bar rotation, a residual liquid part in termination is flowed into reservoir, passes through the heating plate of setting Kill bacterium.
Preferably, the heater strip is tungsten filament, its fusing point is high, resistivity is big, intensity is good.
Preferably, cathode contact-making switch and the cathode contact switch includes plastics seat piece, contact block, upper limit spiral shell Column, lower limit stud, spring and elastic conduction ring;The inoculation bar both sides are equipped with plastics seat piece;Match somebody with somebody in the plastics seat piece Conjunction is connected to contact block;The contact block is used to connect positive plate or negative plate;Described contact block one end is equipped with upper limit Stud;The plastics seat piece bottom is equipped with lower limit stud;Be screwed on spring between the upper limit stud and lower limit stud; The spring periphery covers then elastic conduction ring;The elastic conduction ring side is pierced by plastics seat piece, and is connected to positive terminal On column or negative terminal, after inoculation bar rotation is turned around, the contact block extruding of cathode contact-making switch or cathode contact switch is followed by Contact positive plate or negative plate;And positive terminal, negative terminal and heater strip are connected by elastic conduction ring;Realize heating The function of sterilizing.
Beneficial effects of the present invention are as follows:
1. a kind of bred from the culture of disinfection type microbial bacterial of the present invention is inoculated with instrument, connect by turning round module control Kind bar rotation, switches different termination inoculations, improves conventional efficient;And sterilization module is utilized later in switching, realize inoculation end The sterilization functions of head, carry out sterilizing works without extra, reduce experimenter's workload.
2. it is of the present invention it is a kind of breed inoculation instrument from the culture of disinfection type microbial bacterial, using turning round module setting Pawl-and-gearing, realizes inoculation bar rotation, realizes the switching of inoculation termination, without the inoculating tool with renewing, improve experimenter Work efficiency.
3. a kind of bred from the culture of disinfection type microbial bacterial of the present invention is inoculated with instrument, after inoculation bar rotation is turned around, Cathode contact-making switch connects positive plate, cathode contact switch connection negative plate;Electric-heating-wire-heating is controlled by switching, realizes heating The function of sterilizing, it is simple efficient.
4. a kind of bred from the culture of disinfection type microbial bacterial of the present invention is inoculated with instrument, heater strip uses tungsten filament system Into fusing point is high, resistivity is big, intensity is good, and fever is fast, improves the efficiency of sterilizing.
5. a kind of bred from the culture of disinfection type microbial bacterial of the present invention is inoculated with instrument, by setting reservoir and adding Hot plate, prevents from being stained with the bacterium solution reflux stayed, realizes comprehensive sterilize so that sterilizing is more thorough.
Brief description of the drawings
The invention will be further described below in conjunction with the accompanying drawings.
Fig. 1 is the front view of the present invention;
Fig. 2 is A-A sectional views in Fig. 1;
Fig. 3 is C-C sectional views in Fig. 1;
Fig. 4 is B-B sectional views in Fig. 1;
In figure:Housing 1, inoculation bar 2, reservoir 21, heating plate 22, revolution module 3, shaft 31, ratchet 32, pressing lever 33rd, boss 331, pawl 34, resetting spring 35, sterilization module 4, cathode contact-making switch 41, plastics seat piece 411, contact block 412, Upper limit stud 413, lower limit stud 414, spring 415, elastic conduction ring 416, positive terminal 42, cathode contact switch 43rd, negative terminal 44, heater strip 45, power supply module 5, switch 51, storage battery 52, positive plate 53, negative plate 54.
Embodiment
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention easy to understand, tie below Embodiment is closed, the present invention is further explained.
As shown in Figures 1 to 4, a kind of bred from the culture of disinfection type microbial bacterial of the present invention is inoculated with instrument, including Housing 1, inoculation bar 2, revolution module 3, sterilization module 4 and power supply module 5;Described 1 one end of housing is equipped with notch;In the notch Portion is rotatablely installed inoculation bar 2;2 both ends of the inoculation bar can be inoculated with;The inoculation bar 2 realizes rotation by turning round module 3, Switch different termination inoculations;The revolution module 3 is installed on inside housing 1;The sterilization module 4 is installed on 1 one end of housing It is internal;The power supply module 5 provides electric energy for sterilization module 4, and inside 1 one end of housing;The inoculation bar 2 is by returning Revolving die block 3 realizes rotation, switches different termination inoculations;2 termination of inoculation bar being transferred to inside housing 1, connection circuit, utilizes confession Electric 5 heat sterilization module of module, realizes killing bacteria.
The revolution module 3 includes shaft 31, ratchet 32, pressing lever 33, pawl 34 and resetting spring 35;The shaft 31 The middle part of housing 1 is rotatably installed in, and is fixedly connected on 2 middle part of inoculation bar, described 31 one end of shaft is fixedly connected ratchet 32;Institute Ratchet 32 is stated to be rotatably installed in inside housing 1;The pressing lever 33 is installed inside housings;The ratchet 32 passes through pressing lever 33 The control rotation of pawl 34 of upper setting;Housing 1 is stretched out in described 33 one end of pressing lever, and is equipped with briquetting in termination;The housing 1 Install and be equipped with step in the middle part of the cavity of pressing lever 33;The middle part of pressing plate 33 is equipped with boss 331;Described 331 one end of boss is set There is resetting spring 35;The resetting spring 35 is used to reset after pressing lever 33 presses;The pawl 34 set using pressing lever 33 is driven Dynamic ratchet 32 rotates 180 °;The inoculation bar 2 that ratchet 32 is fixedly connected realizes 180 ° of rotations of rotation, realizes the switching of inoculation termination.
The power supply module 5 includes switch 51, storage battery 52, positive plate 53 and negative plate 54;The switch 51 is installed on 1 side of housing;The storage battery 52 is installed on 1 inside one end of housing;The storage battery 52 is connected to positive plate 53 and negative plate 54;The positive plate 53 is located at 1 notch side of housing;The negative plate 54 is located at 1 notch opposite side of housing.
As one embodiment of the present invention, the sterilization module 4 includes cathode contact-making switch 41, positive terminal 42nd, cathode contact switch 43, negative terminal 44 and heater strip 45;2 both ends of the inoculation bar are equipped with notch;The inoculation bar 2 sides are equipped with cathode contact-making switch 41;The cathode contact-making switch 41 is connected to positive terminal 42;The positive terminal 42 Inside junction pole 2, and in the cylinder of notch side;The cathode contact switch 43 is connected to negative terminal 44; The negative terminal 44 is installed on inside junction pole 2, and in notch opposite side cylinder;The positive terminal 42 and negative Heater strip 45 is connected between pole binding post 44, after inoculation bar 2 rotates, cathode contact-making switch 41 contacts positive plate 53;Anode connects The contact negative plate 54 of switch 43 is touched, connection circuit, realizes that heater strip 45 heats, realize the killing of bacterium.
As one embodiment of the present invention, the positive terminal 42, negative terminal 44 and heater strip 45 connect Place is equipped with reservoir 21;21 both sides of reservoir are equipped with heating plate 22, after inoculation bar 2 rotates, termination residual liquid one Part is flowed into reservoir 21, and bacterium is killed by the heating plate 22 of setting.
As one embodiment of the present invention, the heater strip 45 is tungsten filament, its fusing point is high, resistivity is big, intensity is good.
As one embodiment of the present invention, cathode contact-making switch 41 and the cathode contact switch 43 includes plastics Seat piece 411, contact block 412, upper limit stud 413, lower limit stud 414, spring 415 and elastic conduction ring 416;The inoculation 2 both sides of bar are equipped with plastics seat piece 411;Be connected contact block 412 in the plastics seat piece 411;The contact block 412 is used In contact connectio positive plate 53 or negative plate 54;Described 412 one end of contact block is equipped with upper limit stud 413;The plastics seat piece 411 bottoms are equipped with lower limit stud 414;Be screwed on spring 415 between the upper limit stud 413 and lower limit stud 414;Institute State 415 periphery of spring set then elastic conduction ring 416;416 side of elastic conduction ring is pierced by plastics seat piece 411, and connects On positive terminal 42 or negative terminal 44, after the inoculation rotation of bar 2 is turned around, cathode contact-making switch 41 or cathode contact switch 43 contact block 412 touches positive plate 53 or negative plate 54 after extruding;And positive terminal is connected by elastic conduction ring 416 42nd, negative terminal 44 and heater strip 45;Realize the function of heat sterilization.
During work, operator is used for the inoculation of bacterium first with the termination of inoculation bar 2;Complete after a kind of inoculation, it is necessary to Inoculation termination is replaced, can utilize turn round the rotation inoculation bar 2 of module 3 at this time, i.e., the pawl 34 set using pressing lever 33 is driven Ratchet 32 rotates 180 °;The inoculation bar 2 that ratchet 32 is fixedly connected realizes 180 ° of rotations of rotation, realizes the switching of inoculation termination;It is right In termination inoculated before, sterilize if desired, when rotation at this time due to inoculation bar 2, cathode contact-making switch 41 Or touch positive plate 53 or negative plate 54 after the extruding of contact block 412 of cathode contact switch 43;And pass through elastic conduction ring 416 Positive terminal 42, negative terminal 44 and heater strip 45 are connected, then is only needed by the 51 i.e. connection circuit that compresses switch so that heating Silk 45 produces high temperature, so as to kill bacterium;Above-mentioned circulate operation is realized by turning round the rotation inoculation bar 2 of module 3, improves inoculation Efficiency.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe the original of the present invention Reason, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes and improvements It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle It is fixed.

Claims (5)

1. a kind of breed inoculation instrument from the culture of disinfection type microbial bacterial, it is characterised in that:Including housing (1), inoculation bar (2), Turn round module (3), sterilization module (4) and power supply module (5);Described housing (1) one end is equipped with notch;The notch inner rotation Inoculation bar (2) is installed;The inoculation bar (2) realizes rotation by turning round module (3);The revolution module (3) is installed on shell Body (1) is internal;The sterilization module (4) is installed on inside housing (1) one end;The power supply module (5) carries for sterilization module (4) For electric energy, and inside housing (1) one end;Wherein,
The revolution module (3) includes shaft (31), ratchet (32), pressing lever (33), pawl (34) and resetting spring (35);Institute State shaft (31) to be rotatably installed in the middle part of housing (1), and be fixedly connected in the middle part of inoculation bar (2), described shaft (31) one end is solid Surely it is connected to ratchet (32);It is internal that the ratchet (32) is rotatably installed in housing (1);The pressing lever (33) is installed in housing Portion;The ratchet (32) controls rotation by the pawl (34) set on pressing lever (33);Described pressing lever (33) one end is stretched out Housing (1), and it is equipped with briquetting in termination;Step is equipped with the middle part of the cavity of housing (1) the installation pressing lever (33);It is described to press Boss (331) is equipped with the middle part of pressing plate (33);Described boss (331) one end is equipped with resetting spring (35);The resetting spring (35) Resetted after being pressed for pressing lever (33);
The power supply module (5) includes switch (51), storage battery (52), positive plate (53) and negative plate (54);The switch (51) it is installed on housing (1) side;The storage battery (52) is installed on the internal one end of housing (1);Storage battery (52) connection Positive plate (53) and negative plate (54);The positive plate (53) is located at housing (1) notch side;The negative plate (54) is located at Housing (1) notch opposite side.
2. a kind of bred from the culture of disinfection type microbial bacterial according to claim 1 is inoculated with instrument, it is characterised in that:It is described Sterilization module (4) includes cathode contact-making switch (41), positive terminal (42), cathode contact switch (43), negative terminal (44) and heater strip (45);Described inoculation bar (2) both ends are equipped with notch;Described inoculation bar (2) side is equipped with cathode contact and opens Close (41);The cathode contact-making switch (41) is connected to positive terminal (42);The positive terminal (42) is installed on wiring Bar (2) is internal, and in the cylinder of notch side;The cathode contact switch (43) is connected to negative terminal (44);It is described Negative terminal (44) is installed on junction pole (2) inside, and in notch opposite side cylinder;The positive terminal (42) and Heater strip (45) is connected between negative terminal (44).
3. a kind of bred from the culture of disinfection type microbial bacterial according to claim 2 is inoculated with instrument, it is characterised in that:It is described Positive terminal (42), negative terminal (44) and heater strip (45) junction are equipped with reservoir (21);The reservoir (21) both sides are equipped with heating plate (22).
4. a kind of bred from the culture of disinfection type microbial bacterial according to claim 2 is inoculated with instrument, it is characterised in that:It is described Heater strip (45) is tungsten filament.
5. a kind of bred from the culture of disinfection type microbial bacterial according to claim 2 is inoculated with instrument, it is characterised in that:It is described Cathode contact-making switch (41) and cathode contact switch (43) include plastics seat piece (411), contact block (412), upper limit stud (413), lower limit stud (414), spring (415) and elastic conduction ring (416);Described inoculation bar (2) both sides are equipped with plastics Seat piece (411);Be connected contact block (412) in the plastics seat piece (411);The contact block (412) is used for the company of contact Connect positive plate (53) or negative plate (54);Described contact block (412) one end is equipped with upper limit stud (413);The plastics seat piece (411) bottom is equipped with lower limit stud (414);Be screwed on bullet between the upper limit stud (413) and lower limit stud (414) Spring (415);Spring (415) periphery covers then elastic conduction ring (416);Elastic conduction ring (416) side is pierced by modeling Expect seat piece (411), and be connected on positive terminal (42) or negative terminal (44).
CN201810057759.1A 2018-01-22 2018-01-22 Self-sterilization type microorganism bacterium culture, reproduction and inoculation instrument Active CN107988059B (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108165477A (en) * 2018-03-08 2018-06-15 湖南水口山有色金属集团有限公司 A kind of Quick sterilizing device of microbial inoculant tool
CN114766360A (en) * 2022-04-22 2022-07-22 南通大学 Plant stem segment explant inoculation device

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US4242462A (en) * 1979-03-16 1980-12-30 Thomas Richard E Electrically powered self-heating inoculating loop
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CN202022935U (en) * 2011-04-12 2011-11-02 黑龙江八一农垦大学 Portable self-disinfecting inoculating device
CN102410432A (en) * 2011-11-21 2012-04-11 河北科技大学 Lubricating oil supply device based on self power of mobile part
CN206143208U (en) * 2016-11-09 2017-05-03 齐齐哈尔大学 Microorganism is with inoculation pen
CN206672241U (en) * 2017-04-11 2017-11-24 刘翘楚 One kind takes egg device automatically
CN107475095A (en) * 2017-06-30 2017-12-15 柳州市全品贸易有限公司 Inoculation ring stick with disinfection function

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Publication number Priority date Publication date Assignee Title
US4242462A (en) * 1979-03-16 1980-12-30 Thomas Richard E Electrically powered self-heating inoculating loop
CN2768177Y (en) * 2005-02-02 2006-03-29 梁林坤 Rotary switch
CN201124667Y (en) * 2007-12-03 2008-10-01 华东师范大学附属杨行中学 Multifunctional pen
CN202022935U (en) * 2011-04-12 2011-11-02 黑龙江八一农垦大学 Portable self-disinfecting inoculating device
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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108165477A (en) * 2018-03-08 2018-06-15 湖南水口山有色金属集团有限公司 A kind of Quick sterilizing device of microbial inoculant tool
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CN114766360A (en) * 2022-04-22 2022-07-22 南通大学 Plant stem segment explant inoculation device

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